Comparative analysis of envelope proteomes in Escherichia coli B and K-12 strainsMee Jung Han
Department of Biomolecular and Chemical Engineering, Dongyang University, Gyeongbuk 750 711, Korea
J Microbiol Biotechnol 22:470-8. 2012
..Thus, these findings suggest that E. coli K-12 and its derivatives will be more favorable strains in certain biotechnological applications, such as cell surface display or membrane engineering studies...
Understanding and engineering of microbial cells based on proteomics and its conjunction with other omics studiesMee Jung Han
Department of Biomolecular and Chemical Engineering, Dongyang University, Gyeongbuk, Republic of Korea
Proteomics 11:721-43. 2011
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A systems biology analysis of metastatic melanoma using in-depth three-dimensional protein profilingMee Jung Han
The Wistar Institute, Philadelphia, PA 19104, USA
Proteomics 10:4450-62. 2010
..These results provide novel insights into cellular pathways implicated in melanoma metastasis...
- Extracellular proteome of Aspergillus terreus grown on different carbon sources
Mee Jung Han
Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Institute for the BioCentury, KAIST, Yuseong Gu, Daejeon, Republic of Korea
Curr Genet 56:369-82. 2010
..These proteomic data will be useful for studying protein secretion in further detail, and finding fusion partners for the extracellular production of homologous or heterologous proteins in A. terreus...
- Biotechnological applications of microbial proteomes
Mee Jung Han
Department of Chemical and Biomolecular Engineering, Dongyang University, 1 Gyochon dong, Punggi eup, Yeongju, Gyeongbuk 750 711, Republic of Korea
J Biotechnol 145:341-9. 2010
..Successful applications of proteomic information have proven to have enormous value for both scientific and practical applications...
- Genome-wide identification of the subcellular localization of the Escherichia coli B proteome using experimental and computational methods
Mee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, and Institute for the BioCentury, KAIST, Yuseong Gu, Daejeon, Republic of Korea
Proteomics 11:1213-27. 2011
..coli B, and will be useful as a reference for proteome profiling studies under various conditions. The complete proteome data are available online (http://ecolib.kaist.ac.kr)...
- Comparison of the extracellular proteomes of Escherichia coli B and K-12 strains during high cell density cultivation
Xiao Xia Xia
Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, Yuseong Gu, Daejeon, Republic of Korea
Proteomics 8:2089-103. 2008
..These findings summarized as reference proteome maps will be useful for studying protein release in further detail, and provide new strategies for enhanced excretory production of recombinant proteins...
- Enhanced display of lipase on the Escherichia coli cell surface, based on transcriptome analysis
Jong Hwan Baek
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Dongyang University, Yeongju, Gyeongbuk 750 711, Republic of Korea
Appl Environ Microbiol 76:971-3. 2010
..The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis...
- Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli
Mee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
Biotechnol Bioeng 88:426-36. 2004
..This strategy seems to be generally applicable as it was successfully employed for the enhanced cytosolic or secretory production of several other recombinant proteins in E. coli...
- Microscale solution IEF combined with 2-D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts
Mee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon, Republic of Korea
Electrophoresis 29:695-705. 2008
..Thus the 3-D DIGE strategy produces a powerful method for more comprehensive and more reliable quantitative comparisons of protein profiles of very complex proteomes...
- The Escherichia coli proteome: past, present, and future prospects
Mee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon 305 701, Republic of Korea
Microbiol Mol Biol Rev 70:362-439. 2006
..Here, we review the past development and current status of E. coli proteome research in terms of its biological, biotechnological, and methodological significance and suggest future prospects...
- Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteins
Mee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Daejeon, Republic of Korea
J Proteome Res 4:2429-34. 2005
..Therefore, the use of sHsps during 2-DE significantly improves proteome profiling by generally enabling the detection of many more protein spots that could not be seen previously...
- Microbial small heat shock proteins and their use in biotechnology
Mee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology KAIST, Yuseong Gu, Daejeon 305 701, Republic of Korea
Biotechnol Adv 26:591-609. 2008
..In this review, we discuss the properties and diversity of microbial sHsps, as well as their potential uses in the biotechnology industry...
- Transcriptome and proteome analyses of adaptive responses to methyl methanesulfonate in Escherichia coli K-12 and ada mutant strains
Jong Hwan Baek
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology and Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
BMC Microbiol 9:186. 2009
..Here we present time-dependent global gene and protein expression profiles following treatment with methyl methanesulfonate (MMS) in E. coli W3110 and its ada mutant strains...
- Microscale isoelectric focusing in solution: a method for comprehensive and quantitative proteome analysis using 1-D and 2-D DIGE combined with MicroSol IEF prefractionation
Mee Jung Han
The Wistar Institute, Philadelphia, Pennsylvania, USA
Methods Mol Biol 424:241-56. 2008
..The combination of DIGE and MicroSol IEF produces a powerful method for more comprehensive and quantitative comparison of protein profiles of complex proteomes...
