Brunschweiger A, Gebert L, Lucic M, PradÃ¨re U, Jahns H, Berk C, et al
. Site-specific conjugation of drug-like fragments to an antimiR scaffold as a strategy to target miRNAs inside RISC. Chem Commun (Camb). 2016;52:156-9 pubmed publisher
..At some sites selected fragments elevated cellular antimiR activity to that of an unmodified 23mer antimiR, whereas at others the same fragments abolished activity. The potency of the antimiRs correlated with uptake into miRISC. ..
Li M, Lightfoot H, Halloy F, Malinowska A, Berk C, Behera A, et al
. Synthesis and cellular activity of stereochemically-pure 2'-O-(2-methoxyethyl)-phosphorothioate oligonucleotides. Chem Commun (Camb). 2017;53:541-544 pubmed publisher
..In cells, a full Rp-PS-MOE splice-switching oligonucleotide targeting part of the ferrochelatase gene was more potent than its Sp-PS counterpart, but of similar potency to the stereorandom PS-parent sequence. ..
Lightfoot H, Hall J. Endogenous polyamine function--the RNA perspective. Nucleic Acids Res. 2014;42:11275-90 pubmed publisher
..Most notably, polyamine-mediated effects on RNA are frequently distinct from those of divalent cations (i.e. Mg2+) confirming their roles as independent molecular entities which help drive RNA-mediated processes. ..
Roos M, Rebhan M, Lucic M, Pavlicek D, Pradère U, Towbin H, et al
. Short loop-targeting oligoribonucleotides antagonize Lin28 and enable pre-let-7 processing and suppression of cell growth in let-7-deficient cancer cells. Nucleic Acids Res. 2015;43:e9 pubmed publisher
Menzi M, Pradère U, Wang Y, Fischer M, Baumann F, Bigatti M, et al
. Site-Specific Labeling of MicroRNA Precursors: A Structure-Activity Relationship Study. Chembiochem. 2016;17:2012-2017 pubmed publisher
..Here we describe a systematic investigation of site-specific labeling of pre-miRNAs to identify positions for the incorporation of functional groups, in order not to hinder their processing into active mature miRNAs. ..