Evi S Lianidou
Affiliation: University of Athens
Lianidou E, Mavroudis D, Georgoulias V. Clinical challenges in the molecular characterization of circulating tumour cells in breast cancer. Br J Cancer. 2013;108:2426-32 pubmed publisher
..This review is mainly focused on the clinical potential of the molecular characterisation of CTC in BC. ..
Markou A, Farkona S, Schiza C, Efstathiou T, Kounelis S, Malamos N, et al
. PIK3CA mutational status in circulating tumor cells can change during disease recurrence or progression in patients with breast cancer. Clin Cancer Res. 2014;20:5823-34 pubmed publisher
..Evaluation of PIK3CA mutational status in CTCs is a strategy with potential clinical application. ..
Mastoraki S, Chimonidou M, Dimitrakopoulos L, Kounelis S, Malamos N, Georgoulias V, et al
. A rapid and accurate closed-tube Methylation-Sensitive High Resolution Melting Analysis assay for the semi-quantitative determination of SOX17 promoter methylation in clinical samples. Clin Chim Acta. 2015;444:303-9 pubmed publisher
..It gives comparable results to Real-Time MSP in less time, while it offers the advantage of additionally providing an estimation of SOX17 promoter methylation levels. ..
Wala S, Karamchandani J, Saleeb R, Evans A, Ding Q, Ibrahim R, et al
. An integrated genomic analysis of papillary renal cell carcinoma type 1 uncovers the role of focal adhesion and extracellular matrix pathways. Mol Oncol. 2015;9:1667-77 pubmed publisher
..This novel information can contribute to the development of effective targeted therapies for pRCC1, for which there is none currently available in the clinic. ..
Giannopoulou L, Mastoraki S, Buderath P, Strati A, Pavlakis K, Kasimir Bauer S, et al
. ESR1 methylation in primary tumors and paired circulating tumor DNA of patients with high-grade serous ovarian cancer. Gynecol Oncol. 2018;150:355-360 pubmed publisher
..The agreement between ESR1 methylation in primary tumors and paired ctDNA is statistically significant. Our results indicate a correlation between the presence of ESR1 methylation and a better clinical outcome in HGSC patients. ..
Zavridou M, Mastoraki S, Strati A, Tzanikou E, Chimonidou M, Lianidou E. Evaluation of Preanalytical Conditions and Implementation of Quality Control Steps for Reliable Gene Expression and DNA Methylation Analyses in Liquid Biopsies. Clin Chem. 2018;: pubmed publisher
..Standardization of preanalytical conditions and implementation of quality control steps is extremely important for reliable liquid biopsy analysis, and a prerequisite for routine applications in the clinic. ..
Lianidou E, Markou A, Strati A. The Role of CTCs as Tumor Biomarkers. Adv Exp Med Biol. 2015;867:341-67 pubmed publisher
..We also highlight the importance of performing comparison studies between these different methodologies and external quality control systems for establishing CTCs as tumor biomarkers in the routine clinical setting. ..
Lianidou E. Gene expression profiling and DNA methylation analyses of CTCs. Mol Oncol. 2016;10:431-42 pubmed publisher
..This review is mainly focused on gene expression and methylation studies in CTCs in various types of cancer. ..
Parisi C, Mastoraki S, Markou A, Strati A, Chimonidou M, Georgoulias V, et al
. Development and validation of a multiplex methylation specific PCR-coupled liquid bead array for liquid biopsy analysis. Clin Chim Acta. 2016;461:156-64 pubmed publisher
..The main principle of the developed methodology has the potential to be extended in a large number of gene-targets and be applied in many types of cancer. ..