Microbial metagenomics: beyond the genomeJack A Gilbert
Plymouth Marine Laboratory, Plymouth PL1 3DH, United Kingdom
Ann Rev Mar Sci 3:347-71. 2011
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Metagenomics - a guide from sampling to data analysisTorsten Thomas
School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio Innovation, The University of New South Wales, Sydney, NSW 2052, Australia
Microb Inform Exp 2:3. 2012
..As more metagenomic datasets are generated, the availability of standardized procedures and shared data storage and analysis becomes increasingly important to ensure that output of individual projects can be assessed and compared...
The importance of metagenomic surveys to microbial ecology: or why Darwin would have been a metagenomic scientistJack A Gilbert
Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA
Microb Inform Exp 1:5. 2011
..The fact that evolution remains a 'theory', at least to the general public, suggests that surveys for new data need to be taken to a new level...
Functional analysis of metagenomes and metatranscriptomes using SEED and KEGGSuparna Mitra
Center for Bioinformatics ZBIT, Tubingen University, Sand 14, 72076 Tubingen, Germany
BMC Bioinformatics 12:S21. 2011
..The program has subsequently been extended to support comparative analyses of multiple datasets...
The taxonomic and functional diversity of microbes at a temperate coastal site: a 'multi-omic' study of seasonal and diel temporal variationJack A Gilbert
Plymouth Marine Laboratory, Plymouth, United Kingdom
PLoS ONE 5:e15545. 2010
..Further, the presence of a substantial proportion of orphan sequences underscores the need to determine the gene products of sequences with currently unknown function...
The seasonal structure of microbial communities in the Western English ChannelJack A Gilbert
Plymouth Marine Laboratory, Prospect Place, Plymouth, PL1 3DH, UK
Environ Microbiol 11:3132-9. 2009
..There is also evidence for seasonal succession, primarily reflecting changes among dominant taxa. These changes in structure were significantly correlated to a combination of temperature, phosphate and silicate concentrations...
MetagenomicsJack A Gilbert
Plymouth Marine Laboratory, The Hoe, Plymouth, UK
Methods Mol Biol 733:173-83. 2011
..We hope that the information provided here will be useful to establish the techniques in most reasonably equipped molecular biology laboratories...
Potential for phosphonoacetate utilization by marine bacteria in temperate coastal watersJack A Gilbert
Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth PL1 3DH, UK
Environ Microbiol 11:111-25. 2009
..The fact that the capacity to utilize phosphonoacetate was evident in each of the three coastal environments suggests the potential for widespread utilization of this bioavailable P source...
Bias in assessments of marine microbial biodiversity in fosmid libraries as evaluated by pyrosequencingBen Temperton
Plymouth Marine Laboratory, Plymouth, UK
ISME J 3:792-6. 2009
..This study suggests that, although fosmid libraries can be extremely useful, caution must be taken when directly inferring community composition from metagenomic fosmid libraries...
Defining seasonal marine microbial community dynamicsJack A Gilbert
Plymouth Marine Laboratory, Prospect Place, Plymouth, UK
ISME J 6:298-308. 2012
..Interestingly, microbial association network analysis showed that correlations in abundance were stronger within bacterial taxa rather than between bacteria and eukaryotes, or between bacteria and environmental variables...
Bioturbation: impact on the marine nitrogen cycleBonnie Laverock
Plymouth Marine Laboratory, Prospect Place, Plymouth PL1 3DH, UK
Biochem Soc Trans 39:315-20. 2011
..In the present paper we discuss the potential for bioturbation to significantly affect marine nitrogen cycling, as well as the molecular techniques used to study microbial nitrogen cycling communities and directions for future study...
Detection of large numbers of novel sequences in the metatranscriptomes of complex marine microbial communitiesJack A Gilbert
Plymouth Marine Laboratory, Prospect Place, Plymouth, United Kingdom
PLoS ONE 3:e3042. 2008
..The application of high-throughput sequencing technology is now enabling access to both known and previously unknown transcripts in natural communities...
Draft genome sequence of Agrobacterium albertimagni strain AOL15William L Trimble
Institute for Genomics and Systems Biology, Argonne National Laboratory, Argonne, Illinois, USA
J Bacteriol 194:6986-7. 2012
..1 Mb in 55 contigs with 61.2% GC content and includes a 21-gene arsenic gene island. This is the first available genome for this species and the second Agrobacterium arsenic gene island...
Gene expression profiling: metatranscriptomicsJack A Gilbert
Plymouth Marine Laboratory, The Hoe, Plymouth, UK
Methods Mol Biol 733:195-205. 2011
..Also, a section is dedicated for outlining a bioinformatic procedure for the analysis of metatranscriptomic datasets...
Novel analysis of oceanic surface water metagenomes suggests importance of polyphosphate metabolism in oligotrophic environmentsBen Temperton
School of Biological Sciences, Queen s University Belfast, Belfast, United Kingdom
PLoS ONE 6:e16499. 2011
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Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sedimentsBonnie Laverock
Plymouth Marine Laboratory, Plymouth, UK
ISME J 4:1531-44. 2010
..In an area of high macrofaunal abundance, this could lead to alterations in the microbial transformations of important nutrients at the sediment-water interface...
Isolation and initial characterization of a novel type of Baeyer-Villiger monooxygenase activity from a marine microorganismAndrew Willetts
Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, UK
Microb Biotechnol 5:549-59. 2012
..Although low, abundance was detected in samples from many marine provinces, confirming the potential for biodiscovery in marine microorganisms...
Modeling microbial community structure and functional diversity across time and spacePeter E Larsen
Argonne National Laboratory, Lemont, IL, USA
FEMS Microbiol Lett 332:91-8. 2012
..We show that modeling can help to connect biogeochemical processes to specific microbial metabolic pathways...
Predicting bacterial community assemblages using an artificial neural network approachPeter E Larsen
Argonne National Laboratory Biosciences Division, Illinois, USA
Nat Methods 9:621-5. 2012
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Draft genome sequence of Achromobacter piechaudii strain HLEWilliam L Trimble
Institute for Genomics and Systems Biology, Argonne National Laboratory, Argonne, Illinois, USA
J Bacteriol 194:6355. 2012
..This draft genome of 6.89 Mb is the second available genome for this species in the opportunistic pathogen Alcaligenaceae family...
Predicted Relative Metabolomic Turnover (PRMT): determining metabolic turnover from a coastal marine metagenomic datasetPeter E Larsen
Argonne National Laboratory, 9700, S, Cass Ave, Argonne, Illinois, USA
Microb Inform Exp 1:4. 2011
..abstract:..
A rare SAR11 fosmid clone confirming genetic variability in the 'Candidatus Pelagibacter ubique' genomeJack A Gilbert
Plymouth Marine Laboratory, Prospect Place, Plymouth, UK
ISME J 2:790-3. 2008
..Variation in SAR11 genomes from different locations might explain why SAR11 is abundant in so many diverse marine provinces...