U Brandt

Summary

Affiliation: Zentrum der Biologischen Chemie
Country: Germany

Publications

  1. ncbi request reprint Functional sulfurtransferase is associated with mitochondrial complex I from Yarrowia lipolytica, but is not required for assembly of its iron-sulfur clusters
    Albina Abdrakhmanova
    Ulrich Brandt, Universitat Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Theodor Stern Kai 7, Haus 26, 60590 Frankfurt am Main, Germany
    FEBS Lett 579:6781-5. 2005
  2. pmc Functional dissection of the proton pumping modules of mitochondrial complex I
    Stefan Dröse
    Molecular Bioenergetics Group, Medical School, Cluster of Excellence Frankfurt Macromolecular Complexes, Center for Membrane Proteomics, Johann Wolfgang Goethe Universitat, Frankfurt, Germany
    PLoS Biol 9:e1001128. 2011
  3. ncbi request reprint Proton pumping by NADH:ubiquinone oxidoreductase. A redox driven conformational change mechanism?
    Ulrich Brandt
    Universitat Frankfurt, Fachbereich Medizin, Institut fur Biochemie I, Theodor Stern Kai 7, Haus 25B, D 60590 Frankfurt am Main, Germany
    FEBS Lett 545:9-17. 2003
  4. ncbi request reprint Structure-function relationships in mitochondrial complex I of the strictly aerobic yeast Yarrowia lipolytica
    U Brandt
    Universitat Frankfurt, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Theodor Stern Kai 7, Haus 26, D 60590 Frankfurt am Main, Germany
    Biochem Soc Trans 33:840-4. 2005
  5. ncbi request reprint Energy converting NADH:quinone oxidoreductase (complex I)
    Ulrich Brandt
    Universitat Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, D 60590 Frankfurt am Main, Germany
    Annu Rev Biochem 75:69-92. 2006
  6. ncbi request reprint A two-state stabilization-change mechanism for proton-pumping complex I
    Ulrich Brandt
    Center for Membrane Proteomics, Goethe University, Frankfurt am Main, Germany
    Biochim Biophys Acta 1807:1364-9. 2011
  7. ncbi request reprint Exploring the catalytic core of complex I by Yarrowia lipolytica yeast genetics
    S Kerscher
    , , Zentrum der Biologischen Chemie, Frankfurt am Main, Germany
    J Bioenerg Biomembr 33:187-96. 2001
  8. ncbi request reprint Efficient large scale purification of his-tagged proton translocating NADH:ubiquinone oxidoreductase (complex I) from the strictly aerobic yeast Yarrowia lipolytica
    N Kashani-Poor
    , , ZBC, Theodor-Stern-Kai 7, Haus 25B, D-60590, Frankfurt am Main, Germany
    Biochim Biophys Acta 1504:363-70. 2001
  9. ncbi request reprint Control of ubiquinol oxidation at center P (Qo) of the cytochrome bc1 complex
    U Brandt
    Universitatsklinikum Frankfurt, Institut fur Biochemie I, Zentrum der Biologischen Chemie, Frankfurt am Main, Federal Republic of Germany
    J Bioenerg Biomembr 31:243-50. 1999
  10. ncbi request reprint Role of deprotonation events in ubihydroquinone:cytochrome c oxidoreductase from bovine heart and yeast mitochondria
    U Brandt
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt am Main, Federal Republic of Germany
    Biochemistry 36:11234-40. 1997

Collaborators

Detail Information

Publications75

  1. ncbi request reprint Functional sulfurtransferase is associated with mitochondrial complex I from Yarrowia lipolytica, but is not required for assembly of its iron-sulfur clusters
    Albina Abdrakhmanova
    Ulrich Brandt, Universitat Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Theodor Stern Kai 7, Haus 26, 60590 Frankfurt am Main, Germany
    FEBS Lett 579:6781-5. 2005
    ..In contrast to previous suggestions, this indicated that the sulfurtransferase associated with Y. lipolytica complex I is not required for assembly of its iron-sulfur clusters...
  2. pmc Functional dissection of the proton pumping modules of mitochondrial complex I
    Stefan Dröse
    Molecular Bioenergetics Group, Medical School, Cluster of Excellence Frankfurt Macromolecular Complexes, Center for Membrane Proteomics, Johann Wolfgang Goethe Universitat, Frankfurt, Germany
    PLoS Biol 9:e1001128. 2011
    ..Our results provide evidence that the membrane arm of complex I harbours two functionally distinct pump modules that are connected in series by the long helical transmission element recently identified by X-ray structural analysis...
  3. ncbi request reprint Proton pumping by NADH:ubiquinone oxidoreductase. A redox driven conformational change mechanism?
    Ulrich Brandt
    Universitat Frankfurt, Fachbereich Medizin, Institut fur Biochemie I, Theodor Stern Kai 7, Haus 25B, D 60590 Frankfurt am Main, Germany
    FEBS Lett 545:9-17. 2003
    ..These new insights into structure and function of complex I strongly suggest that proton or sodium pumping in complex I is achieved by conformational energy transfer rather than by a directly linked redox pump...
  4. ncbi request reprint Structure-function relationships in mitochondrial complex I of the strictly aerobic yeast Yarrowia lipolytica
    U Brandt
    Universitat Frankfurt, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Theodor Stern Kai 7, Haus 26, D 60590 Frankfurt am Main, Germany
    Biochem Soc Trans 33:840-4. 2005
    ..Overall, our findings suggest that proton pumping by complex I employs long-range conformational interactions and ubiquinone intermediates play a critical role in this mechanism...
  5. ncbi request reprint Energy converting NADH:quinone oxidoreductase (complex I)
    Ulrich Brandt
    Universitat Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, D 60590 Frankfurt am Main, Germany
    Annu Rev Biochem 75:69-92. 2006
    ..This evolutionary origin is reflected in three functional modules of complex I. The coupling mechanism of complex I most likely involves semiquinone intermediates that drive proton pumping through redox-linked conformational changes...
  6. ncbi request reprint A two-state stabilization-change mechanism for proton-pumping complex I
    Ulrich Brandt
    Center for Membrane Proteomics, Goethe University, Frankfurt am Main, Germany
    Biochim Biophys Acta 1807:1364-9. 2011
    ..This article is part of a Special Issue entitled Allosteric cooperativity in respiratory proteins...
  7. ncbi request reprint Exploring the catalytic core of complex I by Yarrowia lipolytica yeast genetics
    S Kerscher
    , , Zentrum der Biologischen Chemie, Frankfurt am Main, Germany
    J Bioenerg Biomembr 33:187-96. 2001
    ....
  8. ncbi request reprint Efficient large scale purification of his-tagged proton translocating NADH:ubiquinone oxidoreductase (complex I) from the strictly aerobic yeast Yarrowia lipolytica
    N Kashani-Poor
    , , ZBC, Theodor-Stern-Kai 7, Haus 25B, D-60590, Frankfurt am Main, Germany
    Biochim Biophys Acta 1504:363-70. 2001
    ....
  9. ncbi request reprint Control of ubiquinol oxidation at center P (Qo) of the cytochrome bc1 complex
    U Brandt
    Universitatsklinikum Frankfurt, Institut fur Biochemie I, Zentrum der Biologischen Chemie, Frankfurt am Main, Federal Republic of Germany
    J Bioenerg Biomembr 31:243-50. 1999
    ..It is outlined how other aspects of the center P mechanism like the proposed "prosthetic" ubiquinone and the moving domain of the "Rieske" protein could be incorporated in the kinetic framework...
  10. ncbi request reprint Role of deprotonation events in ubihydroquinone:cytochrome c oxidoreductase from bovine heart and yeast mitochondria
    U Brandt
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt am Main, Federal Republic of Germany
    Biochemistry 36:11234-40. 1997
    ..1996) FEBS Lett. 387, 1-6]. All results reported here can be rationalized in a straightforward way based on other aspects of the same hypothesis...
  11. ncbi request reprint Proton-translocation by membrane-bound NADH:ubiquinone-oxidoreductase (complex I) through redox-gated ligand conduction
    U Brandt
    Universitatsklinikum Frankfurt, Zentrum der Biologischen Chemie, Germany
    Biochim Biophys Acta 1318:79-91. 1997
    ..Thus, in the proposed mechanism proton translocation is driven by a direct linkage between redox dependent protonation of iron-sulfur cluster N-2 and the redox chemistry of ubiquinone...
  12. ncbi request reprint The chemistry and mechanics of ubihydroquinone oxidation at center P (Qo) of the cytochrome bc1 complex
    U Brandt
    Universitatsklinikum Frankfurt, Institut für Biochemie 1, Zentrum der Biologischen Chemie, Frankfurt am Main, Germany
    Biochim Biophys Acta 1365:261-8. 1998
    ..This provides strong support for the 'catalytic switch' model proposed earlier based on detailed analysis of inhibitor binding to cytochrome bc1 complex in different redox states...
  13. ncbi request reprint Binding of MOA-stilbene to the mitochondrial cytochrome bc1 complex is affected by the protonation state of a redox-Bohr group of the 'Rieske' iron-sulfur protein
    U Brandt
    Universitatsklinikum Frankfurt, Zentrum der Biologischen Chemie, Frankfurt am Main, Germany
    Biochim Biophys Acta 1321:238-42. 1997
    ..As this second binding site was abolished by the center P inhibitor stigmatellin, but not by antimycin, an inhibitor of ubiquinone reduction at center N, we conclude that it is also located at center P...
  14. ncbi request reprint Bifurcated ubihydroquinone oxidation in the cytochrome bc1 complex by proton-gated charge transfer
    U Brandt
    Universitatsklinikum Frankfurt, Zentrum der Bioligischen Chemie, Frankfurt am Main, Germany
    FEBS Lett 387:1-6. 1996
    ..In this proton-gated charge-transfer mechanism the reaction is controlled by the deprotonation of the substrate ubihydroquinone...
  15. ncbi request reprint The Redox-Bohr group associated with iron-sulfur cluster N2 of complex I
    Klaus Zwicker
    Universitat Frankfurt, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, D 60590 Frankfurt am Main, Germany
    J Biol Chem 281:23013-7. 2006
    ..This finding has significant implications on the discussion about possible proton pumping mechanism for complex I...
  16. ncbi request reprint Functional implications from an unexpected position of the 49-kDa subunit of NADH:ubiquinone oxidoreductase
    Volker Zickermann
    Universitat Frankfurt, Fachbereich Medizin, Institut fur Biochemie I, D 60590 Frankfurt am Main, Germany
    J Biol Chem 278:29072-8. 2003
    ..This contradicts all hypothetical mechanisms discussed in the field that link proton translocation directly to redox events and suggests an indirect mechanism of proton pumping by redox-driven conformational energy transfer...
  17. doi request reprint The mechanism of mitochondrial superoxide production by the cytochrome bc1 complex
    Stefan Dröse
    Molecular Bioenergetics Group, Cluster of Excellence Frankfurt Macromolecular Complexes, Medical School, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    J Biol Chem 283:21649-54. 2008
    ..The resulting mechanism of ROS production offers a straightforward explanation of how the redox state of the ubiquinone pool could play a central role in mitochondrial redox signaling...
  18. doi request reprint Two-dimensional native electrophoretic analysis of respiratory supercomplexes from Yarrowia lipolytica
    Esther Nübel
    Cluster of Excellence Macromolecular Complexes, Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Goethe Universitat Frankfurt, Frankfurt, Germany
    Proteomics 9:2408-18. 2009
    ....
  19. pmc Architecture of complex I and its implications for electron transfer and proton pumping
    Volker Zickermann
    Goethe Universitat, Fachbereich Medizin, Molekulare Bioenergetik, ZBC, Theodor Stern Kai 7, Haus 26, D 60590 Frankfurt am Main, Germany
    Biochim Biophys Acta 1787:574-83. 2009
    ..In this review we compile the available structural information on complex I and discuss implications for complex I function...
  20. doi request reprint Ambivalent effects of diazoxide on mitochondrial ROS production at respiratory chain complexes I and III
    Stefan Dröse
    Molecular Bioenergetics Group, Cluster of Excellence Frankfurt Macromolecular Complexes, Medical School, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    Biochim Biophys Acta 1790:558-65. 2009
    ....
  21. ncbi request reprint Proton translocation in the respiratory chain involving ubiquinone--a hypothetical semiquinone switch mechanism for complex I
    U Brandt
    Universitatsklinikum Frankfurt, Institut fur Biochemie I, Frankfurt am Main, Germany
    Biofactors 9:95-101. 1999
    ..Therefore, a "semiquinone switch" mechanism is proposed involving one tightly bound and one substrate quinone. It is based on the same principles as a Q-cycle, but is a localized rather than a ligand conduction type mechanism...
  22. ncbi request reprint External alternative NADH:ubiquinone oxidoreductase redirected to the internal face of the mitochondrial inner membrane rescues complex I deficiency in Yarrowia lipolytica
    S J Kerscher
    Universitatsklinikum Frankfurt, Institut fur Biochemie I, D 60590 Frankfurt am Main, Federal Republic of Germany
    J Cell Sci 114:3915-21. 2001
    ..Our findings also demonstrate that shuttle mechanisms for the transfer of redox equivalents from the matrix to the cytosolic side of the mitochondrial inner membrane are insufficient in Y. lipolytica...
  23. ncbi request reprint Cluster N1 of complex I from Yarrowia lipolytica studied by pulsed EPR spectroscopy
    T Maly
    Institut für Physikalische und Theoretische Chemie and Center for Biological Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, 60439, Frankfurt am Main, Germany
    J Biol Inorg Chem 11:343-50. 2006
    ....
  24. ncbi request reprint K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
    Stefan Dröse
    Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Universitatsklinikum Frankfurt, Haus 26, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany
    J Biol Chem 281:23733-9. 2006
    ....
  25. ncbi request reprint The proton pumping stoichiometry of purified mitochondrial complex I reconstituted into proteoliposomes
    Alexander Galkin
    Universitat Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Theodor Stern Kai 7, Haus 26, D 60590 Frankfurt am Main, Germany
    Biochim Biophys Acta 1757:1575-81. 2006
    ..6H(+)/2e . This is the first demonstration that the proton pump of complex I stayed fully functional after purification of the enzyme...
  26. ncbi request reprint Mechanism of thiazolidinedione-dependent cell death in Jurkat T cells
    Mathias Soller
    Department of Biochemistry I Pathobiochemistry, Johann Wolfgang Goethe University, Faculty of Medicine, Frankfurt, Main, Germany
    Mol Pharmacol 71:1535-44. 2007
    ..Our results demonstrate that despite their similar molecular structure, thiazolidinediones differently affect cell death, which might help to explain some adverse effects occurring during thiazolidinedione-based therapies...
  27. ncbi request reprint Processing of the 24 kDa subunit mitochondrial import signal is not required for assembly of functional complex I in Yarrowia lipolytica
    Stefan Kerscher
    Universitat Frankfurt, Fachbereich Medizin, Institut fur Biochemie I, Frankfurt am Main, Germany
    Eur J Biochem 271:3588-95. 2004
    ..Complete removal of the NUHM presequence resulted in the absence of complex I activity, strongly arguing against the presence of an internal mitochondrial targeting sequence within the 24 kDa protein...
  28. ncbi request reprint The three families of respiratory NADH dehydrogenases
    Stefan Kerscher
    Molecular Bioenergetics Group, Centre of Excellence Macromolecular Complexes, Johann Wolfgang Goethe Universitat, 60590, Frankfurt am Main, Germany
    Results Probl Cell Differ 45:185-222. 2008
    ..This review gives an overview of the origin, structural and functional properties and physiological significance of these three types of NADH dehydrogenase...
  29. doi request reprint Characterization of two different acyl carrier proteins in complex I from Yarrowia lipolytica
    Krzysztof Dobrynin
    Molecular Bioenergetics Group, Medical School, Cluster of Excellence Frankfurt Macromolecular Complexes, Centre for Membrane Proteomics, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    Biochim Biophys Acta 1797:152-9. 2010
    ..Hence we conclude that the ACPMs can serve all their possible functions in mitochondrial lipid metabolism and complex I assembly and stabilization as subunits bound to complex I...
  30. ncbi request reprint Superoxide radical formation by pure complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica
    Alexander Galkin
    Universitat Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, D 60590 Frankfurt am Main, Germany
    J Biol Chem 280:30129-35. 2005
    ..From the effect of different ubiquinone derivatives and pH on this side reaction of complex I we concluded that oxygen accepts electrons from FMNH2 or FMN semiquinone either directly or via more hydrophilic ubiquinone derivatives...
  31. ncbi request reprint Application of the yeast Yarrowia lipolytica as a model to analyse human pathogenic mutations in mitochondrial complex I (NADH:ubiquinone oxidoreductase)
    Stefan Kerscher
    Johann Wolfgang Goethe, Universitat Frankfurt, Fachbereich Medizin, Institut fur Biochemie I, ZBC, Molekulaire Bioenergetik, Theodor Stern Kai 7, Haus 25B, Frankfurt am Main, D 60590, Germany
    Biochim Biophys Acta 1659:197-205. 2004
    ..Thus, Y. lipolytica is a useful lower eucaryotic model that will help to understand how pathogenic mutations in complex I interfere with enzyme function...
  32. ncbi request reprint Subunit composition of mitochondrial complex I from the yeast Yarrowia lipolytica
    Albina Abdrakhmanova
    Fachbereich Medizin, Institut fur Biochemie I, ZBC, Universitat Frankfurt, Theodor Stern Kai 7, Haus 25B, D 60590 Frankfurt am Main, Germany
    Biochim Biophys Acta 1658:148-56. 2004
    ..Its position within the membrane arm was determined by electron microscopic single particle analysis of Y. lipolytica complex I decorated with a NUWM-specific monoclonal antibody...
  33. ncbi request reprint Two aspartic acid residues in the PSST-homologous NUKM subunit of complex I from Yarrowia lipolytica are essential for catalytic activity
    Aurelio Garofano
    Johann Wolfgang Goethe Universität Frankfurt am Main, Fachbereich Medizin, Gustav Embden Zentrum der Biologischen Chemie, Institut fur Biochemie I, Theodor Stern Kai 7, Haus 25B, D 60590 Frankfurt am Main, Germany
    J Biol Chem 278:42435-40. 2003
    ..The results are discussed in the light of the structural similarities to the homologous small subunit of water-soluble [NiFe] hydrogenases...
  34. ncbi request reprint Functional significance of conserved histidines and arginines in the 49-kDa subunit of mitochondrial complex I
    Ljuban Grgic
    Institut fur Biochemie I, Fachbereich Medizin, Universitat Frankfurt, Theodor Stern Kai 7, Haus 25B, D 60590 Frankfurt am Main, Germany
    J Biol Chem 279:21193-9. 2004
    ..Our results question some of the central mechanistic functions that have been put forward for iron-sulfur cluster N2...
  35. ncbi request reprint Significance of respirasomes for the assembly/stability of human respiratory chain complex I
    Hermann Schagger
    Institut fur Biochemie I, Zentrum der Biologischen Chemie, Fachbereich Medizin, Universitat Frankfurt, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany
    J Biol Chem 279:36349-53. 2004
    ..This dependence of complex I assembly/stability on respirasome formation has important implications for the diagnosis of mitochondrial respiratory chain disorders...
  36. ncbi request reprint Yarrowia lipolytica, a yeast genetic system to study mitochondrial complex I
    Stefan Kerscher
    Universitatsklinikum Frankfurt, Institut fur Biochemie I, Zentrum der Biologischen Chemie, Theodor Stern Kai 7, Haus 25 B, D 60590 Frankfurt am Main, Germany
    Biochim Biophys Acta 1555:83-91. 2002
    ..lipolytica complex I. Characterization of a number of mutations already allowed for the identification of several functionally important amino acids, demonstrating the usefulness of this approach...
  37. ncbi request reprint A single external enzyme confers alternative NADH:ubiquinone oxidoreductase activity in Yarrowia lipolytica
    S J Kerscher
    Institut fur Biochemie I, Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt am Main, Germany
    J Cell Sci 112:2347-54. 1999
    ..cerevisiae...
  38. ncbi request reprint Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules
    J Ludwig
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt, Germany
    J Biol Chem 273:10939-47. 1998
    ..It shows sequence and structural similarity to Vma21p, a yeast protein required for assembly of vacuolar ATPase. A second membrane sector-associated protein (M8-9) was identified and characterized by amino-terminal protein sequencing...
  39. doi request reprint Subunit mass fingerprinting of mitochondrial complex I
    Nina Morgner
    Institute of Physical and Theoretical Chemistry, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    Biochim Biophys Acta 1777:1384-91. 2008
    ..Thus, the LILBID subunit mass fingerprint method will be of great value for efficient proteomic analysis of complex I and its assembly intermediates, as well as of other water soluble and membrane bound multiprotein complexes...
  40. ncbi request reprint Cardiolipin stabilizes respiratory chain supercomplexes
    Kathy Pfeiffer
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt, Germany
    J Biol Chem 278:52873-80. 2003
    ..We show that a cardiolipin-deficient strain harbored almost inactive resting cytochrome c oxidase in the membrane. Transition to the fully active pulsed state occurred on a minute time scale...
  41. ncbi request reprint Exploring the inhibitor binding pocket of respiratory complex I
    Uta Fendel
    Johann Wolfgang Goethe Universitat, Fachbereich Medizin, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Cluster of Excellence Frankfurt Macromolecular Complexes, Frankfurt am Main, Germany
    Biochim Biophys Acta 1777:660-5. 2008
    ..Taken together, our data provide structure-based support for the presence of distinct but overlapping binding sites for hydrophobic inhibitors possibly extending into the ubiquinone reduction site of mitochondrial complex I...
  42. ncbi request reprint Small single transmembrane domain (STMD) proteins organize the hydrophobic subunits of large membrane protein complexes
    Volker Zickermann
    Goethe Universitat, Fachbereich Medizin, Molekulare Bioenergetik, Cluster of Excellence Frankfurt Macromolecular Complexes, Frankfurt am Main, Germany
    FEBS Lett 584:2516-25. 2010
    ..We hypothesize that the general function of STMD subunits is to organize the hydrophobic subunits of large membrane protein complexes in specialized environments like the inner mitochondrial membrane...
  43. ncbi request reprint Substrate-inducible versions of internal alternative NADH: ubiquinone oxidoreductase from Yarrowia lipolytica
    Aurelio Garofano
    Universitat Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, Theodor Stern Kai 7, Haus 26, 60590 Frankfurt am Main, Germany
    Yeast 23:1129-36. 2006
    ..In the presence of complex I inhibitors, this construct allowed growth on acetate, but not on glucose minimal media. A somewhat higher background was observed with the acyl-CoA oxidase 2 (pPOX2) promoter on glucose minimal media...
  44. ncbi request reprint Histidine 129 in the 75-kDa subunit of mitochondrial complex I from Yarrowia lipolytica is not a ligand for [Fe4S4] cluster N5 but is required for catalytic activity
    Antje Waletko
    Universitat Frankfurt, Fachbereich Medizin, Institut fur Biochemie I, F 60590 Frankfurt am Main, Germany
    J Biol Chem 280:5622-5. 2005
    ..Deamino-NADH: ubiquinone oxidoreductase activity was abolished completely by the mutation. However, EPR spectroscopic analysis of mutant complex I exhibited an unchanged cluster N5 signal, excluding histidine 129 as a cluster N5 ligand...
  45. ncbi request reprint Exploring the ubiquinone binding cavity of respiratory complex I
    Maja A Tocilescu
    Johann Wolfgang Goethe Universitat, Fachbereich Medizin, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Centre of Excellence Frankfurt Macromolecular Complexes, D 60590 Frankfurt am Main, Germany
    J Biol Chem 282:29514-20. 2007
    ..Finally, we were able to highlight a functionally critical structural motif in the active site that consisted of Tyr-144 in the 49-kDa subunit, surrounded by three conserved hydrophobic residues...
  46. pmc Identification of the mitochondrial ND3 subunit as a structural component involved in the active/deactive enzyme transition of respiratory complex I
    Alexander Galkin
    Molecular Bioenergetics Group, Cluster of Excellence Frankfurt Macromolecular Complexes, Medical School, Johann Wolfgang Goethe Universitat, Theodor Stern Kai 7, Frankfurt am Main, Germany
    J Biol Chem 283:20907-13. 2008
    ..In fact, mutations in three positions of the loop were previously reported to cause Leigh syndrome with and without dystonia or progressive mitochondrial disease...
  47. ncbi request reprint Energy conservation by bifurcated electron-transfer in the cytochrome-bc1 complex
    U Brandt
    Universitatsklinikum Frankfurt, Zentrum der Biologischen Chemie, Germany
    Biochim Biophys Acta 1275:41-6. 1996
    ..In this proton-gated charge-transfer mechanism the reaction is controlled by the deprotonation of the substrate ubihydroquinone and not, as proposed earlier, by the formation of a highly unstable semiquinone species...
  48. ncbi request reprint Ubiquinol-cytochrome-c reductase from human and bovine mitochondria
    H Schagger
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, Frankfurt am Main, Germany
    Methods Enzymol 260:82-96. 1995
  49. ncbi request reprint Proton pumping by complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica reconstituted into proteoliposomes
    Stefan Dröse
    Universitat Frankfurt, Fachbereich Medizin, Molekulare Bioenergetik, Theodor Stern Kai 7, Haus 26, D 60590 Frankfurt am Main, Germany
    Biochim Biophys Acta 1710:87-95. 2005
    ..While this study was not specifically designed to demonstrate possible additional sodium translocating properties of complex I, we did not find indications for primary or secondary Na+ translocation by Y. lipolytica complex I...
  50. doi request reprint Challenges in elucidating structure and mechanism of proton pumping NADH:ubiquinone oxidoreductase (complex I)
    Volker Zickermann
    Fachbereich Medizin, Molekulare Bioenergetik, Cluster of Excellence Frankfurt, Macromolecular Complexes, ZBC, Goethe Universitat, Frankfurt am Main, Germany
    J Bioenerg Biomembr 40:475-83. 2008
    ..The physiological role of this phenomenon is yet unclear but it could contribute to the regulation of complex I activity in-vivo...
  51. ncbi request reprint Full recovery of the NADH:ubiquinone activity of complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica by the addition of phospholipids
    Stefan Dröse
    Institut für Biochemie I Molekulare Bioenergetik, Fachbereich Medizin, Johann Wolfgang Goethe Universitat, Theodor Stern Kai 7, Haus 25B, D 60590, Frankfurt am Main, Germany
    Biochim Biophys Acta 1556:65-72. 2002
    ..As the purified enzyme was also found to contain only substoichiometric amounts of ubiquinone-9 (0.2-0.4 mol/mol), a functional requirement of this endogeneous ubiquinone could also be excluded...
  52. ncbi request reprint HDQ (1-hydroxy-2-dodecyl-4(1H)quinolone), a high affinity inhibitor for mitochondrial alternative NADH dehydrogenase: evidence for a ping-pong mechanism
    Andrea Eschemann
    Universitat Frankfurt, Fachbereich Medizin, Institut fur Biochemie I, Frankfurt am Main D 60490, Germany
    J Biol Chem 280:3138-42. 2005
    ..We found that the kinetics of alternative NADH dehydrogenase follow a ping-pong mechanism. This suggests that NADH and the ubiquinone headgroup interact with the same binding pocket in an alternating fashion...
  53. ncbi request reprint Tight binding of NADPH to the 39-kDa subunit of complex I is not required for catalytic activity but stabilizes the multiprotein complex
    Albina Abdrakhmanova
    Universitat Frankfurt, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Theodor Stern Kai 7, Haus 26, D 60590 Frankfurt am Main, Germany
    Biochim Biophys Acta 1757:1676-82. 2006
    ..We concluded that the 39-kDa subunit of Y. lipolytica plays a critical role in complex I assembly and stability and that the bound NADPH serves to stabilize the subunit and complex I as a whole rather than serving a catalytic function...
  54. doi request reprint Laser-induced liquid bead ion desorption-MS of protein complexes from blue-native gels, a sensitive top-down proteomic approach
    Lucie Sokolova
    Molecular Bioenergetics Group, Medical School, Cluster of Excellence Frankfurt Macromolecular Complexes, Centre for Membrane Proteomics, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    Proteomics 10:1401-7. 2010
    ..Starting from a subcellular fraction obtained by differential centrifugation, this allowed the purification and analysis of the constituents of a large multiprotein complex in a single LILBID spectrum...
  55. doi request reprint Chapter 26 Measurement of superoxide formation by mitochondrial complex I of Yarrowia lipolytica
    Stefan Dröse
    Molecular Bioenergetics Group, Cluster of Excellence Frankfurt Macromolecular Complexes, Medical School, Johann Wolfgang Goethe University, Frankfurt am Main, Germany
    Methods Enzymol 456:475-90. 2009
    ..ROS production by Yarrowia complex I in the "forward mode" is essentially independent of catalytic turnover, membrane potential, and the presence of inhibitors or the active/deactive transition...
  56. ncbi request reprint Characterization of a subcomplex of mitochondrial NADH:ubiquinone oxidoreductase (complex I) lacking the flavoprotein part of the N-module
    Volker Zickermann
    Johann Wolfgang Goethe Universitat, Fachbereich Medizin, Molekulare Bioenergetik, Centre of Excellence Frankfurt Macromolecular Complexes, D 60590 Frankfurt am Main, Germany
    Biochim Biophys Acta 1767:393-400. 2007
    ....
  57. ncbi request reprint Mitochondrial dysfunction, peroxidation damage and changes in glutathione metabolism in PARK6
    Hans Hermann Hoepken
    Section for Molecular Neurogenetics, Clinic for Neurology, University Hospital, Theodor Stern Kai 7, 60590 Frankfurt M, Germany
    Neurobiol Dis 25:401-11. 2007
    ..These data indicate that PINK1 function is critical to prevent oxidative damage and that peripheral cells may be useful for studies of progression and therapy of PARK6...
  58. doi request reprint A common mechanism links differently acting complex II inhibitors to cardioprotection: modulation of mitochondrial reactive oxygen species production
    Stefan Dröse
    Molecular Bioenergetics Group, Cluster of Excellence Frankfurt Macromolecular Complexes, Medical School, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    Mol Pharmacol 79:814-22. 2011
    ....
  59. doi request reprint Phospholipase A2-modified low density lipoprotein induces mitochondrial uncoupling and lowers reactive oxygen species in phagocytes
    Dmitry Namgaladze
    Goethe University, Faculty of Medicine, Institute of Biochemistry I ZAFES, Theodor Stern Kai 7, Frankfurt, Germany
    Atherosclerosis 208:142-7. 2010
    ..Thus, PLA-LDL-induced uncoupling attenuates reactive oxygen species generation, which may contribute to increased monocyte survival in atherosclerotic plaques and support pro-atherogenic effects of LDL modified by PLA(2)...
  60. ncbi request reprint Mitochondrial complex I is deficient in renal oncocytomas
    Helene Simonnet
    CGMC Center of Molecular and Cell Genetics, Unit 5534 of the CNRS and the University Lyon 1 Claude Bernard, Villeurbanne, France
    Carcinogenesis 24:1461-6. 2003
    ..This suggested that oncocytomas are the result of at least two serial modifications altering the mitochondrial respiratory chain...
  61. ncbi request reprint Analysis of dichlorodihydrofluorescein and dihydrocalcein as probes for the detection of intracellular reactive oxygen species
    Alexandra Keller
    Institut für Kardiovaskuläre Physiologie, Klinikum der JW Goethe Universität, Theodor Stern Kai 7, D 60590 Frankfurt am Main, Germany
    Free Radic Res 38:1257-67. 2004
    ..These observations indicate that H2-DCF is an indicator for intracellular ROS, whereas the oxidation of H2-calcein most likely occurs as a consequence of direct electron transfer to mitochondrial complex I...
  62. pmc K(ATP) channel-independent targets of diazoxide and 5-hydroxydecanoate in the heart
    Peter J Hanley
    Institut fur Normale und Pathologische Physiologie, Universitat Marburg, Deutschhausstrasse 2, 35037 Marburg, Germany
    J Physiol 542:735-41. 2002
    ..We propose that pharmacological preconditioning may be related to partial inhibition of respiratory chain complexes...
  63. pmc 5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for beta-oxidation of fatty acids
    Peter J Hanley
    Institut fur Normale und Pathologische Physiologie, Universitat Marburg, Deutschhausstrasse 2, 35037 Marburg, Germany
    J Physiol 562:307-18. 2005
    ..This creates a bottleneck for beta-oxidation of fatty acids. The complex metabolic effects of 5-HD invalidate the use of 5-HD as a blocker of mitochondrial K(ATP) channels in studies of preconditioning...
  64. pmc Direct localization of the 51 and 24 kDa subunits of mitochondrial complex I by three-dimensional difference imaging
    Todd Clason
    University of Vermont, College of Medicine, Department Molecular Physiology and Biophysics, Burlington, VT 05405, USA
    J Struct Biol 159:433-42. 2007
    ....
  65. ncbi request reprint ATR-FTIR redox difference spectroscopy of Yarrowia lipolytica and bovine complex I
    Douglas Marshall
    Glynn Laboratory of Bioenergetics, Department of Biology, University College London, UK
    Biochemistry 45:5458-67. 2006
    ..This is the first report of IR properties of ubiquinone in complex I, and the data could be used to estimate a stoichiometry of 0.2-0.4 per complex I...
  66. ncbi request reprint Synthesis and inhibitory action of novel acetogenin mimics with bovine heart mitochondrial complex I
    Takeshi Hamada
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Biochemistry 43:3651-8. 2004
    ..Therefore, Deltalac-acetogenins can be regarded as a novel type of inhibitor acting on the terminal electron-transfer step of complex I...
  67. ncbi request reprint Heterocyclic analogues of squamocin as inhibitors of mitochondrial complex I. On the role of the terminal lactone of annonaceous acetogenins
    Romain A Duval
    Laboratoire de Pharmacognosie BioCIS, UMR 8076 CNRS, Faculte de Pharmacie, University Paris Sud, rue J B Clement, 92296 Chatenay Malabry Cedex, France
    Biochemistry 45:2721-8. 2006
    ..This result supports the existence of a large cooperatively regulated inhibitor/ubiquinone-binding pocket located within the catalytic core of the enzyme, consisting of the association of the previously defined affinity sites A and B...
  68. ncbi request reprint Subcomplexes of human ATP synthase mark mitochondrial biosynthesis disorders
    Rosalba Carrozzo
    Unit of Molecular Medicine, Bambino Gesù Hospital and Research Institute, Rome, Italy
    Ann Neurol 59:265-75. 2006
    ..Methods: We describe biochemically and clinically relevant aspects of mitochondrial ATP synthase, the enzyme that supplies most ATP for the cells energy demand...
  69. pmc Halothane, isoflurane and sevoflurane inhibit NADH:ubiquinone oxidoreductase (complex I) of cardiac mitochondria
    Peter J Hanley
    Institut für Normale und Pathologische Physiologie der Universität Marburg, Deutschhausstrasse 2, Germany
    J Physiol 544:687-93. 2002
    ..These inhibitory effects are unlikely to compromise cardiac performance at usual clinical concentrations, but may contribute to the mechanism by which volatile anaesthetics induce pharmacological preconditioning...
  70. ncbi request reprint Relaxation filtered hyperfine (REFINE) spectroscopy: a novel tool for studying overlapping biological electron paramagnetic resonance signals applied to mitochondrial complex I
    Thorsten Maly
    Institut für Physikalische und Theoretische Chemie and Zentrum für Biomolekulare Magnetische Resonanz, Johann Wolfgang Goethe Universitat Frankfurt, D 60439 Frankfurt am Main, Germany
    Biochemistry 43:3969-78. 2004
    ..Finally, REFINE is used to assign the observed nitrogen modulation in complex I to an individual iron-sulfur cluster...
  71. pmc The iron-sulphur protein Ind1 is required for effective complex I assembly
    Katrine Bych
    Department of Plant Sciences, University of Cambridge, Cambridge, UK
    EMBO J 27:1736-46. 2008
    ..Our data suggest that Ind1 facilitates the assembly of Fe-S cofactors and subunits of complex I...
  72. ncbi request reprint Semisynthesis and screening of a small library of pro-apoptotic squamocin analogues: selection and study of a benzoquinone hybrid with an improved biological profile
    Séverine Derbré
    Laboratoire de Pharmacognosie Associé au CNRS BioCIS, UMR 8076, Universite Paris Sud 11, Centre d Etudes Pharmaceutiques, 5 Rue Jean Baptiste Clement, 92296 Chatenay Malabry Cedex, France
    ChemMedChem 1:118-29. 2006
    ..Squamoquinone, tenfold more potent than squamocin as an inducer of apoptosis, emerged as a promising compound, as it induces apoptosis through a mitochondrial caspase-dependent pathway...
  73. ncbi request reprint Proteomic and functional analyses reveal a mitochondrial dysfunction in P301L tau transgenic mice
    Della C David
    Division of Psychiatry Research, University of Zurich, 8008 Zurich, Switzerland
    J Biol Chem 280:23802-14. 2005
    ..Taken together, we conclude that tau pathology involves a mitochondrial and oxidative stress disorder possibly distinct from that caused by Abeta...
  74. pmc Secondary mitochondrial dysfunction in propionic aciduria: a pathogenic role for endogenous mitochondrial toxins
    Marina A Schwab
    Department of General Pediatrics, Division of Inborn Metabolic Diseases, University Children s Hospital Heidelberg, Im Neuenheimer Feld 150, D 69120 Heidelberg, Germany
    Biochem J 398:107-12. 2006
    ....
  75. ncbi request reprint Remarkable substituent effect: beta-aminosquamocin, a potent dual inhibitor of mitochondrial complexes I and III
    Romain A Duval
    Laboratoire de Pharmacognosie Associé au CNRS UMR 8076, BIOCIS, Centre d Etudes Pharmaceutiques, Universite Paris Sud 11, 92296 Chatenay Malabry Cedex, France
    Biochim Biophys Acta 1709:191-4. 2005
    ..The mechanism of action of beta-aminosquamocin 2, against these two respiratory targets, is studied and discussed in view of current structure-activity relationship knowledge in the acetogenin series...