Stefan Weger

Summary

Country: Germany

Publications

  1. ncbi request reprint The DNA topoisomerase I binding protein topors as a novel cellular target for SUMO-1 modification: characterization of domains necessary for subcellular localization and sumolation
    Stefan Weger
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Exp Cell Res 290:13-27. 2003
  2. ncbi request reprint Topors acts as a SUMO-1 E3 ligase for p53 in vitro and in vivo
    Stefan Weger
    Institute of Infectious Diseases, Department of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    FEBS Lett 579:5007-12. 2005
  3. ncbi request reprint Topors, a p53 and topoisomerase I binding protein, interacts with the adeno-associated virus (AAV-2) Rep78/68 proteins and enhances AAV-2 gene expression
    Stefan Weger
    Institut für Infektionsmedizin, Abteilung Virologie, Freie Universitat Berlin, Hindenburgdamm 27, D 12203 Berlin, Germany
    J Gen Virol 83:511-6. 2002
  4. ncbi request reprint The E3 ligase Topors induces the accumulation of polysumoylated forms of DNA topoisomerase I in vitro and in vivo
    Eva Hammer
    Institute of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    FEBS Lett 581:5418-24. 2007
  5. ncbi request reprint Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2)
    Stefan Weger
    Institut fur Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Virology 362:192-206. 2007
  6. ncbi request reprint SUMO-1 modification regulates the protein stability of the large regulatory protein Rep78 of adeno associated virus type 2 (AAV-2)
    Stefan Weger
    Institut für Infektionsmedizin, Abteilung Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, 12203 Berlin, Germany
    Virology 330:284-94. 2004
  7. pmc Packaging of human chromosome 19-specific adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production
    Daniela Hüser
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, 12203 Berlin, Germany
    J Virol 77:4881-7. 2003
  8. pmc Role of the herpes simplex virus helicase-primase complex during adeno-associated virus DNA replication
    Heiko Slanina
    Institut fur Virologie, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 27, 12203 Berlin, Germany
    J Virol 80:5241-50. 2006
  9. pmc ssDNA-dependent colocalization of adeno-associated virus Rep and herpes simplex virus ICP8 in nuclear replication domains
    Regine Heilbronn
    Institut für Infektionsmedizin, Abt Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Germany
    Nucleic Acids Res 31:6206-13. 2003
  10. pmc Kinetics and frequency of adeno-associated virus site-specific integration into human chromosome 19 monitored by quantitative real-time PCR
    Daniela Hüser
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Berlin, Germany
    J Virol 76:7554-9. 2002

Collaborators

  • Regine Heilbronn
  • Markus Engstler
  • Frank J Kaiser
  • Henry Fechner
  • Daniela Hüser
  • Mario Mietzsch
  • Eva Hammer
  • Isaac Sipo
  • Heiko Slanina
  • Antonette Bennett
  • Nicholas Muzyczka
  • Sergei Zolotukhin
  • Sabrina Grasse
  • Mavis Agbandje-McKenna
  • Catherine Zurawski
  • Xiaomin Wang
  • Nigel D Stow
  • Wolfgang Poller
  • Iver Petersen
  • Annette Kuhrs
  • Almudena Hurtado Picó
  • Jürgen Eberle

Detail Information

Publications14

  1. ncbi request reprint The DNA topoisomerase I binding protein topors as a novel cellular target for SUMO-1 modification: characterization of domains necessary for subcellular localization and sumolation
    Stefan Weger
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Exp Cell Res 290:13-27. 2003
    ..One SUMO-1 acceptor site at lysine residue 560 could be identified within this region. However, sumolation-deficient Topors mutants showed that sumolation obviously is not required for localization to nuclear speckles...
  2. ncbi request reprint Topors acts as a SUMO-1 E3 ligase for p53 in vitro and in vivo
    Stefan Weger
    Institute of Infectious Diseases, Department of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    FEBS Lett 579:5007-12. 2005
    ..In HeLa cells, Topors induced p53 sumoylation was accompanied by an increase in endogenous p53 protein levels. Furthermore, Topors enhances the sumoylation of a variety of other, yet unidentified, cellular proteins...
  3. ncbi request reprint Topors, a p53 and topoisomerase I binding protein, interacts with the adeno-associated virus (AAV-2) Rep78/68 proteins and enhances AAV-2 gene expression
    Stefan Weger
    Institut für Infektionsmedizin, Abteilung Virologie, Freie Universitat Berlin, Hindenburgdamm 27, D 12203 Berlin, Germany
    J Gen Virol 83:511-6. 2002
    ..Overexpression of Topors stimulated AAV gene expression in the absence of helper virus, suggesting a function of Topors as a transcriptional regulator...
  4. ncbi request reprint The E3 ligase Topors induces the accumulation of polysumoylated forms of DNA topoisomerase I in vitro and in vivo
    Eva Hammer
    Institute of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    FEBS Lett 581:5418-24. 2007
    ..Experiments with TOP1 point mutants strongly suggest that the high-molecular weight conjugates represent SUMO-1 chains formed on a limited number of SUMO-1 acceptor sites...
  5. ncbi request reprint Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2)
    Stefan Weger
    Institut fur Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Virology 362:192-206. 2007
    ..Our study expands the panel of already identified nuclear Rep interaction partners to a cytoplasmic protein, which raises the awareness that important steps in the AAV life cycle may be regulated in this compartment...
  6. ncbi request reprint SUMO-1 modification regulates the protein stability of the large regulatory protein Rep78 of adeno associated virus type 2 (AAV-2)
    Stefan Weger
    Institut für Infektionsmedizin, Abteilung Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, 12203 Berlin, Germany
    Virology 330:284-94. 2004
    ..This finding implicates a role for sumolation in the regulation of Rep78 protein stability that is assumed to be critical for the establishment and maintenance of AAV latency...
  7. pmc Packaging of human chromosome 19-specific adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production
    Daniela Hüser
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, 12203 Berlin, Germany
    J Virol 77:4881-7. 2003
    ..Apparently, AAV latency established by site-specific integration and the helper virus-dependent, productive AAV cycle are more closely related than previously thought...
  8. pmc Role of the herpes simplex virus helicase-primase complex during adeno-associated virus DNA replication
    Heiko Slanina
    Institut fur Virologie, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 27, 12203 Berlin, Germany
    J Virol 80:5241-50. 2006
    ....
  9. pmc ssDNA-dependent colocalization of adeno-associated virus Rep and herpes simplex virus ICP8 in nuclear replication domains
    Regine Heilbronn
    Institut für Infektionsmedizin, Abt Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Germany
    Nucleic Acids Res 31:6206-13. 2003
    ..This novel mechanism for recruiting components of a functional replication complex directs AAV to subnuclear HSV replication compartments where the HSV replication complex can replicate the AAV genome...
  10. pmc Kinetics and frequency of adeno-associated virus site-specific integration into human chromosome 19 monitored by quantitative real-time PCR
    Daniela Hüser
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Berlin, Germany
    J Virol 76:7554-9. 2002
    ..Use of this quantitative real-time PCR will greatly facilitate the study of the early steps of wild-type and recombinant AAV vector integration...
  11. pmc OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy
    Mario Mietzsch
    1 Institute of Virology, Campus Benjamin Franklin, Charite Universitatsmedizin Berlin, 12203 Berlin, Germany
    Hum Gene Ther 25:212-22. 2014
    ..Thus, OneBac combines full AAV serotype options with the capacity for stable scale-up production, the current bottleneck for the transition of AAV from gene therapy trials to routine clinical treatment...
  12. ncbi request reprint A bidirectional Tet-dependent promotor construct regulating the expression of E1A for tight control of oncolytic adenovirus replication
    Henry Fechner
    Department of Cardiology and Pulmology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    J Biotechnol 127:560-74. 2007
    ..Future improvement of the Dox-dependent E1A regulation cassette should lead to the generation of an oAdV well suited to meet the demands for a highly regulated and efficient oncolytic virus for in vivo applications...
  13. ncbi request reprint SUMOylation modulates transcriptional repression by TRPS1
    Frank J Kaiser
    Institut fur Humangenetik, Universitatsklinikum Essen, D 45122 Essen, Germany
    Biol Chem 388:381-90. 2007
    ..These results identify SUMOylation at the TRPS1-RD as a major mechanism that regulates the function of TRPS1...
  14. ncbi request reprint An improved Tet-On regulatable FasL-adenovirus vector system for lung cancer therapy
    Isaac Sipo
    Department of Cardiology and Pneumology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin Germany
    J Mol Med (Berl) 84:215-25. 2006
    ..These data show that the novel TRE-Tight1 promoter has a high potential for closely controlled and efficient expression of cytotoxic genes in AdV-based anti-cancer approaches...