Stefan Wagner

Summary

Country: Germany

Publications

  1. doi request reprint Non-peptidyl (18)F-labelled PET tracers as radioindicators for the noninvasive detection of cancer
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital Munster, Munster, Germany
    Recent Results Cancer Res 187:107-32. 2013
  2. ncbi request reprint Novel fluorinated derivatives of the broad-spectrum MMP inhibitors N-hydroxy-2(R)-[[(4-methoxyphenyl)sulfonyl](benzyl)- and (3-picolyl)-amino]-3-methyl-butanamide as potential tools for the molecular imaging of activated MMPs with PET
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital of the Westfälische Wilhelms Universität Münster, Germany
    J Med Chem 50:5752-64. 2007
  3. ncbi request reprint Molecular imaging of matrix metalloproteinases in vivo using small molecule inhibitors for SPECT and PET
    S Wagner
    Department of Nuclear Medicine, University Hospital Munster, Munster, Germany
    Curr Med Chem 13:2819-38. 2006
  4. doi request reprint A new 18F-labelled derivative of the MMP inhibitor CGS 27023A for PET: radiosynthesis and initial small-animal PET studies
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Str 33, D 48149 Munster, Germany
    Appl Radiat Isot 67:606-10. 2009
  5. doi request reprint The MMP inhibitor (R)-2-(N-benzyl-4-(2-[18F]fluoroethoxy)phenylsulphonamido)-N-hydroxy-3-methylbutanamide: Improved precursor synthesis and fully automated radiosynthesis
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital Munster, Germany
    Appl Radiat Isot 69:862-8. 2011
  6. ncbi request reprint Synthesis and first in vivo evaluation of new selective high affinity beta1-adrenoceptor radioligands for SPECT based on ICI 89,406
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Strasse 33, 48149 Munster, Germany
    Bioorg Med Chem 12:4117-32. 2004
  7. doi request reprint Radiofluorinated pyrimidine-2,4,6-triones as molecular probes for noninvasive MMP-targeted imaging
    Hans Jörg Breyholz
    Department of Nuclear Medicine, University of Munster, Germany
    ChemMedChem 5:777-89. 2010
  8. ncbi request reprint C-5-disubstituted barbiturates as potential molecular probes for noninvasive matrix metalloproteinase imaging
    Hans Jörg Breyholz
    Department of Nuclear Medicine, University Hospital of the Westfälische Wilhelms Universität, Munster, Germany
    J Med Chem 48:3400-9. 2005
  9. doi request reprint Synthesis and evaluation of a novel hydroxamate based fluorescent photoprobe for imaging of matrix metalloproteinases
    Andreas Faust
    Department of Clinical Radiology, Albert Schweitzer Strasse 33, University Hospital Munster, Munster, Germany
    Bioconjug Chem 20:904-12. 2009
  10. ncbi request reprint Scintigraphic imaging of matrix metalloproteinase activity in the arterial wall in vivo
    Michael Schäfers
    Department of Nuclear Medicine, Hospital of the Westfälische Wilhelms University, Munster, Germany
    Circulation 109:2554-9. 2004

Collaborators

Detail Information

Publications60

  1. doi request reprint Non-peptidyl (18)F-labelled PET tracers as radioindicators for the noninvasive detection of cancer
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital Munster, Munster, Germany
    Recent Results Cancer Res 187:107-32. 2013
    ..Furthermore, concrete clinical perspectives are given, that refer to highly potent and promising oncological radiotracers which are currently in preclinical development...
  2. ncbi request reprint Novel fluorinated derivatives of the broad-spectrum MMP inhibitors N-hydroxy-2(R)-[[(4-methoxyphenyl)sulfonyl](benzyl)- and (3-picolyl)-amino]-3-methyl-butanamide as potential tools for the molecular imaging of activated MMPs with PET
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital of the Westfälische Wilhelms Universität Münster, Germany
    J Med Chem 50:5752-64. 2007
    ..As expected, the biodistribution behavior of this novel compound and that of the more hydrophilic variant [18F]1j, also developed by our group, indicates that there was no tissue specific accumulation in wild-type (WT) mice...
  3. ncbi request reprint Molecular imaging of matrix metalloproteinases in vivo using small molecule inhibitors for SPECT and PET
    S Wagner
    Department of Nuclear Medicine, University Hospital Munster, Munster, Germany
    Curr Med Chem 13:2819-38. 2006
    ..Subsequently, the development of radiolabelled small molecule MMPIs, their synthesis and in vitro and in vivo evaluation is reviewed. Finally, an outlook on the clinical potential of labelled MMPIs in diagnostic algorithms is given...
  4. doi request reprint A new 18F-labelled derivative of the MMP inhibitor CGS 27023A for PET: radiosynthesis and initial small-animal PET studies
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Str 33, D 48149 Munster, Germany
    Appl Radiat Isot 67:606-10. 2009
    ..Initial small-animal PET studies in wild-type mice (C57/BL6) showed no unfavourable tissue accumulation of [(18)F]1a...
  5. doi request reprint The MMP inhibitor (R)-2-(N-benzyl-4-(2-[18F]fluoroethoxy)phenylsulphonamido)-N-hydroxy-3-methylbutanamide: Improved precursor synthesis and fully automated radiosynthesis
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital Munster, Germany
    Appl Radiat Isot 69:862-8. 2011
    ..3%. Furthermore, the target compound [(18)F]9 was prepared with a radiochemical yield of 14.8±3.9% (not corrected for decay)...
  6. ncbi request reprint Synthesis and first in vivo evaluation of new selective high affinity beta1-adrenoceptor radioligands for SPECT based on ICI 89,406
    Stefan Wagner
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Strasse 33, 48149 Munster, Germany
    Bioorg Med Chem 12:4117-32. 2004
    ..Therefore further studies in a different animal model will be carried out...
  7. doi request reprint Radiofluorinated pyrimidine-2,4,6-triones as molecular probes for noninvasive MMP-targeted imaging
    Hans Jörg Breyholz
    Department of Nuclear Medicine, University of Munster, Germany
    ChemMedChem 5:777-89. 2010
    ..The radiosynthesis and the in vivo biodistribution of the first (18)F-labeled prototype, MMP-targeted tracer [(18)F]26, suitable for molecular imaging by means of positron emission tomography (PET) were realized...
  8. ncbi request reprint C-5-disubstituted barbiturates as potential molecular probes for noninvasive matrix metalloproteinase imaging
    Hans Jörg Breyholz
    Department of Nuclear Medicine, University Hospital of the Westfälische Wilhelms Universität, Munster, Germany
    J Med Chem 48:3400-9. 2005
    ..We here describe a set of fine-tuned barbiturates as a new class of MMPI radiotracers for the noninvasive in vivo visualization of activated MMPs using scintigraphic techniques such as SPECT or PET...
  9. doi request reprint Synthesis and evaluation of a novel hydroxamate based fluorescent photoprobe for imaging of matrix metalloproteinases
    Andreas Faust
    Department of Clinical Radiology, Albert Schweitzer Strasse 33, University Hospital Munster, Munster, Germany
    Bioconjug Chem 20:904-12. 2009
    ..In conclusion, this new CGS-based MMP photoprobe has a high affinity for MMP-2 and -9 and is thus a promising candidate for sensitive imaging of MMP activity in various diseases in patients...
  10. ncbi request reprint Scintigraphic imaging of matrix metalloproteinase activity in the arterial wall in vivo
    Michael Schäfers
    Department of Nuclear Medicine, Hospital of the Westfälische Wilhelms University, Munster, Germany
    Circulation 109:2554-9. 2004
    ..Matrix metalloproteinases (MMPs) are enzymes involved in the proteolytic degradation of extracellular matrix. They play an important role in several disease processes, such as inflammation, cancer, and atherosclerosis...
  11. ncbi request reprint Synthesis and preliminary biological evaluation of new radioiodinated MMP inhibitors for imaging MMP activity in vivo
    Klaus Kopka
    Department of Nuclear Medicine, University Hospital of the Westfälische Wilhelms Universität, Muenster, Germany
    Nucl Med Biol 31:257-67. 2004
    ..The preliminary biological evaluation warrant further studies of these radioiodinated MMP inhibitors as potential new radiotracers for imaging MMP activity in vivo...
  12. doi request reprint A new class of highly potent matrix metalloproteinase inhibitors based on triazole-substituted hydroxamates: (radio)synthesis and in vitro and first in vivo evaluation
    Verena Hugenberg
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Campus 1, Building A1, D 48149 Munster, Germany
    J Med Chem 55:4714-27. 2012
    ..The biodistribution behavior of this novel compound was investigated with small animal PET...
  13. doi request reprint Inverse 1,2,3-triazole-1-yl-ethyl substituted hydroxamates as highly potent matrix metalloproteinase inhibitors: (radio)synthesis, in vitro and first in vivo evaluation
    Verena Hugenberg
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Campus 1, Building A1, D 48149 Munster, Germany
    J Med Chem 56:6858-70. 2013
    ..Pharmacokinetic properties and metabolic stability of an (18)F-labeled candidate in mice were investigated. ..
  14. doi request reprint A new generation of radiofluorinated pyrimidine-2,4,6-triones as MMP-targeted radiotracers for positron emission tomography
    Daniela Schrigten
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Campus 1, Building A1, D 48149 Munster, Germany
    J Med Chem 55:223-32. 2012
    ....
  15. ncbi request reprint 5-pyrrolidinylsulfonyl isatins as a potential tool for the molecular imaging of caspases in apoptosis
    Klaus Kopka
    Department of Nuclear Medicine, University Hospital of the Westfälische Wilhelms Universität, Munster, Germany
    J Med Chem 49:6704-15. 2006
    ..The 2-methoxymethylpyrrolidinyl versions of the isatins appear to possess superior caspase inhibition potencies in cellular apoptosis inhibition assays compared with the 2-phenoxymethylpyrrolidinyl inhibitors...
  16. ncbi request reprint Are [O-methyl-11C]derivatives of ICI 89,406 beta1-adrenoceptor selective radioligands suitable for PET?
    Marilyn P Law
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Str 33, 48149, Munster, Germany
    Eur J Nucl Med Mol Imaging 35:174-85. 2008
    ..The aim was to evaluate ICI 89,406, a beta(1)-AR-selective antagonist amenable to labelling with positron emitters, for PET...
  17. doi request reprint Synthesis and evaluation of a novel fluorescent photoprobe for imaging matrix metalloproteinases
    Andreas Faust
    Department of Clinical Radiology and Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Str 33, 48149 Munster, Germany
    Bioconjug Chem 19:1001-8. 2008
    ..Therefore, this new barbiturate-based MMP-probe has a high affinity and specificity toward MMP-2 and -9 and is thus a promising candidate for sensitive MMP detection in vivo...
  18. doi request reprint Development and evaluation of endothelin-A receptor (radio)ligands for positron emission tomography
    Kristin Michel
    Department of Nuclear Medicine, University Hospital Münster, Albert Schweitzer Strasse 33, D 48149 Münster, Germany
    J Med Chem 54:939-48. 2011
    ..Improved clearance properties and specific uptake in target organs have been confirmed by biodistribution studies and small animal PET imaging...
  19. doi request reprint PET-compatible endothelin receptor radioligands: synthesis and first in vitro and in vivo studies
    Carsten Höltke
    Department of Nuclear Medicine, Albert Schweitzer Str 33, University Hospital Munster, D 48149 Munster, Germany
    Bioorg Med Chem 17:7197-208. 2009
    ..The biodistribution studies showed a high accumulation of the tracer in bile and intestine. In vivo we were able to show that the visualization of the heart as a major target organ with high ET(A)R expression is possible...
  20. doi request reprint Molecular imaging of cardiac sympathetic innervation by 11C-mHED and PET: from man to mouse?
    Marilyn P Law
    Department of Nuclear Medicine, University Hospital, University of Munster, Munster, Germany
    J Nucl Med 51:1269-76. 2010
    ..The present study assesses the feasibility of using [N-methyl-(11)C]meta-hydroxyephedrine ((11)C-mHED) to measure norepinephrine reuptake in humans, to determine cardiac innervation in mice...
  21. ncbi request reprint Synthesis, in vitro pharmacology and biodistribution studies of new PD 156707-derived ET(A) receptor radioligands
    Carsten Höltke
    Department of Nuclear Medicine, Albert Schweitzer Str 33, University Hospital Munster, 48149 Munster, Germany
    Bioorg Med Chem 14:1910-7. 2006
    ..Biodistribution studies and scintigraphic imaging experiments in mice, however, showed no significant uptake of the [123I] derivative in the heart...
  22. doi request reprint Synthesis, 18F-radiolabeling, and in vivo biodistribution studies of N-fluorohydroxybutyl isatin sulfonamides using positron emission tomography
    Panupun Limpachayaporn
    Organisch Chemisches Institut, Westfalische Wilhelms Universitat Munster, Corrensstrasse 40, D 48149 Munster, Germany
    J Med Chem 56:4509-20. 2013
    ..In vivo biodistribution studies in wild-type mice using PET/computed tomography imaging proved fast clearance of the tracer after tail vein injection...
  23. doi request reprint Efficient synthesis of a fluorine-18 labeled biotin derivative
    Michael Claesener
    University Hospital Munster, Department of Nuclear Medicine, Albert Schweitzer Campus 1, Building A1, 48149 Munster, Germany
    Nucl Med Biol 39:1189-94. 2012
    ..We have synthesized a fluorine-18 labeled biotin derivative [(18)F]4 for a potential application in positron emission tomography (PET)...
  24. pmc Preclinical evaluation of an 18F-labelled beta1-adrenoceptor selective radioligand based on ICI 89,406
    Marilyn P Law
    Department of Nuclear Medicine, University Hospital Munster, D 48149 Munster, Germany
    Nucl Med Biol 37:517-26. 2010
    ..We have chosen ICI 89,406, a beta(1)-selective AR antagonist, as the lead structure to develop new beta(1)-AR radioligands for PET and have synthesised a fluoro-ethoxy derivative (F-ICI)...
  25. doi request reprint Fluorinated isatin derivatives. Part 3. New side-chain fluoro-functionalized pyrrolidinyl sulfonyl isatins as potent caspase-3 and -7 inhibitors
    Anil Kumar Podichetty
    Organisch Chemisches Institut, Westfalische Wilhelms Universitat, Corrensstraße 40, D 48149 Munster, Germany
    Future Med Chem 1:969-89. 2009
    ..Effector caspases such as caspases-3 and -7 get activated during the apoptotic signaling cascade and hence represent a biological target for the diagnosis and therapy of apoptosis-associated diseases...
  26. doi request reprint Fluorinated isatin derivatives. Part 2. New N-substituted 5-pyrrolidinylsulfonyl isatins as potential tools for molecular imaging of caspases in apoptosis
    Anil K Podichetty
    Organisch Chemisches Institut and International NRW Graduate School of Chemistry, Westfalische Wilhelms Universitat, Munster, Germany
    J Med Chem 52:3484-95. 2009
    ..The radiochemistry utilized in the (18)F-fluorination reverted to carrier-added [(18)F]Et(3)N.3HF, a new fluorine-18 source for radiolabeling...
  27. doi request reprint Fluorinated isatin derivatives. Part 1: synthesis of new N-substituted (S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatins as potent caspase-3 and -7 inhibitors
    Anil Kumar Podichetty
    Organisch Chemisches Institut and International NRW Graduate School of Chemistry, Westfalische Wilhelms Universitat, Corrensstrasse 40, D 48149 Munster, Germany
    Bioorg Med Chem 17:2680-8. 2009
    ..Fluorinated N-benzyl isatins as well as trifluoroalkyl and difluoroalkyl derivatives were moderate inhibitors. However, isatins bearing different alkylether groups at N-1 are very weak or not active as inhibitors of caspases-3 and -7...
  28. doi request reprint (18)F-labelled cardiac PET tracers: selected probes for the molecular imaging of transporters, receptors and proteases
    Klaus Kopka
    Dept of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Str 33, 48149, Munster, Germany
    Basic Res Cardiol 103:131-43. 2008
    ..e. beta-Adrenoceptors), transporters (i.e. NET and VMAT) and proteases (i.e. MMPs)...
  29. doi request reprint Radiosynthesis of a ⁶⁸Ga labeled matrix metalloproteinase inhibitor as a potential probe for PET imaging
    Michael Claesener
    University Hospital Munster, Department of Nuclear Medicine, Albert Schweitzer Campus 1, Building A1, 48149 Munster, Germany
    Appl Radiat Isot 70:1723-8. 2012
    ..But, in this case, the strain induced cycloaddition of the reported pre-labeled cyclooctyne-DOTA derivative (68)Ga-1 with azides was proven to be slow at room temperature and heating was necessary for acceptable reaction times...
  30. ncbi request reprint Design of new beta1-selective adrenoceptor ligands as potential radioligands for in vivo imaging
    Klaus Kopka
    Department of Nuclear Medicine, Albert Schweitzer Str 33, University Hospital Munster, 48149, Munster, Germany
    Bioorg Med Chem 11:3513-27. 2003
    ....
  31. doi request reprint Synthesis of geminal difluorides by oxidative desulfurization-difluorination of alkyl aryl thioethers with halonium electrophiles in the presence of fluorinating reagents and its application for 18F-radiolabeling
    Verena Hugenberg
    Organisch Chemisches Institut, Westfalische Wilhelms Universitat, Corrensstrasse 40, D 48149 Munster, Germany
    J Org Chem 75:6086-95. 2010
    ..Using the combination of DBH and carrier-added Py·9H[(18)F]F, an (18)F-labeled difluoride was synthesized from the corresponding α-fluoro thioether with a radiochemical yield of 9%...
  32. doi request reprint Encapsulating (111)In in nanocontainers for scintigraphic imaging: synthesis, characterization, and in vivo biodistribution
    Manuel M Tsotsalas
    Physikalisches Institut and NRW Graduate School of Chemistry, Westfalische Wilhelms Universitat Munster, Mendelstrasse 7, D 48149 Munster, Germany
    ACS Nano 4:342-8. 2010
    ..This strategy leads to a nanoimaging probe with a very high density of radioisotopes in a confined space, which is highly stable in physiological solution and could allow a large variety of functionalities on its external surface...
  33. ncbi request reprint Molecular imaging of apoptosis in vivo with scintigraphic and optical biomarkers--a status report
    Andreas Faust
    Department of Nuclear Medicine, University Hospital Munster, Albert Schweitzer Str 33, D 48149 Munster, Germany
    Anticancer Agents Med Chem 9:968-85. 2009
    ....
  34. ncbi request reprint Molecular cardiovascular imaging using scintigraphic methods
    Lars Stegger
    Department of Nuclear Medicine, University Hospital of Munster, Albert Schweitzer Str 33, 48149 Munster, Germany
    Eur Radiol 17:1422-32. 2007
    ..The focus lies on imaging of cardiac innervation, plaque instability, hypoxia and angiogenesis, gene expression and stem and progenitor cell migration and proliferation...
  35. ncbi request reprint A 18F-radiolabeled analogue of CGS 27023A as a potential agent for assessment of matrix-metalloproteinase activity in vivo
    H J Breyholz
    Department of Nuclear Medicine, University Hospital Munster, Munster, Germany
    Q J Nucl Med Mol Imaging 51:24-32. 2007
    ..A diagnostic tool for the non-invasive detection of MMP activity in vivo is based on MMP inhibitor (MMPI) radiotracers...
  36. ncbi request reprint Non-invasive approaches to visualize the endothelin axis in vivo using state-of-the-art molecular imaging modalities
    C Höltke
    Department of Nuclear Medicine, University Hospital Munster, Munster, Germany
    Mini Rev Med Chem 9:1580-95. 2009
    ..This review summarizes the work that has been published in the past ten years using labeled endothelin receptor ligands for the visualization of endothelin receptor expression in vivo...
  37. ncbi request reprint Non-invasive molecular imaging of beta-adrenoceptors in vivo: perspectives for PET-radioligands
    K Kopka
    Department of Nuclear Medicine, University Hospital of the Westfälische Wilhelms Universität, Munster, Germany
    Curr Med Chem 12:2057-74. 2005
    ..Non-invasive quantification of beta-ARs could facilitate the accurate choice and control of therapeutic interventions. Here we summarize the state-of-the-art of the radiochemistry of radioactive beta-AR radioligands...
  38. pmc Early assessment of the efficacy of temozolomide chemotherapy in experimental glioblastoma using [18F]FLT-PET imaging
    Thomas Viel
    European Institute for Molecular Imaging EIMI, Westfälische Wilhelms University WWU, Munster, Germany
    PLoS ONE 8:e67911. 2013
    ..Our aim was to monitor early effects of TMZ in a mouse model of GBM using positron emission tomography (PET) with 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT)...
  39. doi request reprint Diverse modifications of the 4-methylphenyl moiety of TAK-779 by late-stage Suzuki-Miyaura cross-coupling
    Anna Junker
    Institut für Pharmazeutische und Medizinische Chemie der Universität Münster, Corrensstr 48, D 48149 Munster, Germany
    Org Biomol Chem 12:177-86. 2014
    ..The Suzuki-Miyaura cross-coupling reactions allowed various modifications of the central amide building block 3 at the end of the synthesis leading to compounds 2f and 2h with a promising CCR5 binding affinity. ..
  40. ncbi request reprint The nonpeptidyl caspase binding radioligand (S)-1-(4-(2-[18F]Fluoroethoxy)-benzyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin ([18F]CbR) as potential positron emission tomography-compatible apoptosis imaging agent
    A Faust
    Department of Nuclear Medicine, University Hospital Muenster, Muenster, Germany
    Q J Nucl Med Mol Imaging 51:67-73. 2007
    ....
  41. ncbi request reprint Metastatic neuroblastoma demonstrated by whole-body PET-CT using 11C-HED
    C Franzius
    Department of Nuclear Medicine, University Hospital, Munster, Germany
    Nuklearmedizin 44:N4-5. 2005
  42. doi request reprint Beta1-adrenoceptors in rat anterior pituitary may be constitutively active. Inverse agonism of CGP 20712A on basal 3',5'-cyclic adenosine 5'-monophosphate levels
    Kristel Janssens
    Laboratory of Cell Pharmacology, University of Leuven, Medical School, Campus Gasthuisberg O and N, B 3000 Leuven, Belgium
    Endocrinology 149:2391-402. 2008
    ..CGP 20712A may act as an inverse agonist with approximately 50% negative intrinsic activity, suggesting that the beta(1)-AR significantly contributes to basal adenylate cyclase activity in the pituitary...
  43. doi request reprint Ranolazine improves diastolic dysfunction in isolated myocardium from failing human hearts--role of late sodium current and intracellular ion accumulation
    Samuel Sossalla
    Heart Center, Georg August University Gottingen, Germany
    J Mol Cell Cardiol 45:32-43. 2008
    ..These results suggest that ranolazine may be of therapeutic benefit in conditions of diastolic dysfunction due to elevated [Na(+)](i) and diastolic [Ca(2+)](i)...
  44. ncbi request reprint Inhibition of tumor cell growth by antibodies induced after vaccination with peptides derived from the extracellular domain of Her-2/neu
    Joanna Jasinska
    BioLife Science GmbH, Vienna, Austria
    Int J Cancer 107:976-83. 2003
    ..We conclude from our data that immunization with Her-2/neu peptides successfully induced humoral immune response with anti-tumor activity in an animal model...
  45. ncbi request reprint Induction of protein translation by ADAR1 within living cell nuclei is not dependent on RNA editing
    Alan Herbert
    Department of Neurology, E633, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
    Mol Cell 10:1235-46. 2002
    ..Point mutations within each domain are identified that reduce nuclear translation; those in the DRBM region are also known to diminish RNA binding. This report adds to the growing functionality ascribed to the nucleus...
  46. ncbi request reprint Selection of mimotopes of the cell surface adhesion molecule Mel-CAM from a random pVIII-28aa phage peptide library
    Christine Hafner
    Department of Dermatology, Division of General Dermatology, University of Vienna, Austria
    J Invest Dermatol 119:865-9. 2002
    ..Our results clearly indicate that these 28mer peptides are structural equivalents of the MAd18-5D7 epitope of Mel-CAM and that they will be useful tools for further in vitro and in vivo studies of Mel-CAM mediated cell-cell interaction...
  47. pmc The spatial targeting and nuclear matrix binding domains of SRm160
    Stefan Wagner
    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 100:3269-74. 2003
    ..Sequences from the N-terminal domain localized proteins to the nuclear lamina near sites where mRNA leaves the nucleus...
  48. ncbi request reprint Expression of the B subunit of the heat-labile enterotoxin of Escherichia coli in tobacco mosaic virus-infected Nicotiana benthamiana plants and its characterization as mucosal immunogen and adjuvant
    Birgit Wagner
    Department of Pathophysiology, Medical University of Vienna, General Hospital Vienna EBO 3 Q, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    J Immunol Methods 287:203-15. 2004
    ..In conclusion, these results indicate that the plant viral expression system is suitable for the rapid large-scale production of biologically active LTB with strong mucosal adjuvant capacity...
  49. ncbi request reprint Generation of functional cardiomyocytes from adult mouse spermatogonial stem cells
    Kaomei Guan
    Department of Cardiology and Pneumology, Georg August University of Gottingen, Gottingen, Germany
    Circ Res 100:1615-25. 2007
    ..No tumor formation was found up to 1 month after cell transplantation. Taken together, we believe that maGSCs provide a new source of distinct types of cardiomyocytes for basic research and potential therapeutic application...
  50. ncbi request reprint Excessive sarcoplasmic/endoplasmic reticulum Ca2+-ATPase expression causes increased sarcoplasmic reticulum Ca2+ uptake but decreases myocyte shortening
    Nils Teucher
    Department of Cardiology and Pneumology, University of Goettingen, Goettingen, Germany
    Circulation 110:3553-9. 2004
    ..We investigated the effects of different levels of SERCA1a expression on contractility and Ca2+ cycling. We tested whether increased SERCA1a expression levels enhance myocyte contractility in a gene-dose-dependent manner...
  51. ncbi request reprint Suppression of human melanoma tumor growth in SCID mice by a human high molecular weight-melanoma associated antigen (HMW-MAA) specific monoclonal antibody
    Christine Hafner
    Department of Dermatology, Division of General Dermatology, Medical University of Vienna, Vienna, Austria
    Int J Cancer 114:426-32. 2005
    ..28S. Our results suggest that anti-HMW-MAA mAbs may represent useful reagents to apply passive immunotherapy to patients with malignant melanoma...
  52. ncbi request reprint Enhancement of oxygen surface exchange kinetics of SrTiO(3) by alkaline earth metal oxides
    Christos Argirusis
    TU Clausthal, Institut für Metallurgie, Robert Koch Str 42, 38678, Clausthal Zell, Germany
    Phys Chem Chem Phys 7:3523-5. 2005
    ..The surface of the undoped SrTiO(3) single crystals was modified by alkaline earth metal compounds leading to a tremendous enhancement of the effective surface exchange rate for oxygen incorporation as compared to the uncoated surface...
  53. doi request reprint Allergens are distributed into few protein families and possess a restricted number of biochemical functions
    Christian Radauer
    Department of Pathophysiology, Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, Vienna, Austria
    J Allergy Clin Immunol 121:847-52.e7. 2008
    ..Existing allergen databases classify their entries by source and route of exposure, thus lacking an evolutionary, structural, and functional classification of allergens...
  54. ncbi request reprint Single-chain antibodies to the EWS NH(2) terminus structurally discriminate between intact and chimeric EWS in Ewing's sarcoma and interfere with the transcriptional activity of EWS in vivo
    Dave N T Aryee
    Children s Cancer Research Institute, Vienna, Austria
    Cancer Res 66:9862-9. 2006
    ..Thus, we have generated a tool that will prove useful to specifically differentiate between normal and rearranged EWS in functional studies...
  55. ncbi request reprint PTEN enters the nucleus by diffusion
    Fenghua Liu
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Cell Biochem 96:221-34. 2005
    ..We conclude that PTEN enters the nucleus by diffusion. In addition, sequestration of PTEN in the cytoplasm likely limits PTEN nuclear translocation...
  56. pmc Ca2+/calmodulin-dependent protein kinase II regulates cardiac Na+ channels
    Stefan Wagner
    Department of Cardiology and Pneumology, Georg August University Gottingen, Gottingen, Germany
    J Clin Invest 116:3127-38. 2006
    ..In mice, enhanced CaMKIIdelta(C) activity predisposed to VT. Thus, CaMKII-dependent regulation of Na(+) channel function may contribute to arrhythmogenesis in HF...
  57. pmc Persistent STAT3 activation in colon cancer is associated with enhanced cell proliferation and tumor growth
    Florian M Corvinus
    Institute of Biochemistry I, Friedrich Schiller University Jena Medical School, Jena, Germany
    Neoplasia 7:545-55. 2005
    ..Blockade of STAT3 activation in CRC-derived xenograft tumors slowed down their development, arguing for a contribution of STAT3 to colorectal tumor growth...
  58. ncbi request reprint Increased SR Ca2+ cycling contributes to improved contractile performance in SERCA2a-overexpressing transgenic rats
    Lars S Maier
    Abt Kardiologie and Pneumologie Herzzentrum, Georg August Universitat Gottingen, Germany
    Cardiovasc Res 67:636-46. 2005
    ..We reported that SERCA2a-overexpression in transgenic rat hearts (TG) results in improved contractility. However, it was not clear whether TG have improved contractility due to frequency-dependent improved SR Ca2+ handling...
  59. pmc Simulation of Ca-calmodulin-dependent protein kinase II on rabbit ventricular myocyte ion currents and action potentials
    Eleonora Grandi
    Biomedical Engineering Laboratory, Department of Electronics, Computer Science and Systems, University of Bologna, Bologna, Italy
    Biophys J 93:3835-47. 2007
    ..This provides a useful initial framework to consider pathways by which CaMKII may contribute to arrhythmogenesis...
  60. ncbi request reprint Pluripotency of spermatogonial stem cells from adult mouse testis
    Kaomei Guan
    Department of Cardiology and Pneumology, Heart Center, Georg August University of Gottingen, Robert Koch Str 40, 37075 Gottingen, Germany
    Nature 440:1199-203. 2006
    ..Furthermore, these cells may provide new opportunities to study genetic diseases in various cell lineages...