Christof von Kalle

Summary

Country: Germany

Publications

  1. pmc Real-time definition of non-randomness in the distribution of genomic events
    Ulrich Abel
    Department of Translational Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg, Germany Department of Medical Biostatistics, Tumor Center Heidelberg Mannheim, Heidelberg, Germany
    PLoS ONE 2:e570. 2007
  2. pmc Prospective, randomized, double-blind, multi-center, Phase III clinical study on transarterial chemoembolization (TACE) combined with Sorafenib versus TACE plus placebo in patients with hepatocellular cancer before liver transplantation - HeiLivCa [ISRCTN
    K Hoffmann
    Department of Surgery, Ruprecht Karls University, Heidelberg, Germany
    BMC Cancer 8:349. 2008
  3. doi request reprint Stable long-term blood formation by stem cells in murine steady-state hematopoiesis
    Oksana Zavidij
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany
    Stem Cells 30:1961-70. 2012
  4. pmc Insertion sites in engrafted cells cluster within a limited repertoire of genomic areas after gammaretroviral vector gene therapy
    Annette Deichmann
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center DKFZ, Heidelberg, Germany
    Mol Ther 19:2031-9. 2011
  5. pmc Gammaretrovirus-mediated correction of SCID-X1 is associated with skewed vector integration site distribution in vivo
    Kerstin Schwarzwaelder
    National Center for Tumor Diseases, German Cancer Research Center, Heidelberg, Germany
    J Clin Invest 117:2241-9. 2007
  6. pmc Extensive methylation of promoter sequences silences lentiviral transgene expression during stem cell differentiation in vivo
    Friederike Herbst
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, Heidelberg, Germany
    Mol Ther 20:1014-21. 2012
  7. pmc Bioinformatic clonality analysis of next-generation sequencing-derived viral vector integration sites
    Anne Arens
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    Hum Gene Ther Methods 23:111-8. 2012
  8. doi request reprint Comprehensive genomic access to vector integration in clinical gene therapy
    Richard Gabriel
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany
    Nat Med 15:1431-6. 2009
  9. pmc Analyzing the number of common integration sites of viral vectors--new methods and computer programs
    Ulrich Abel
    Department of Medical Biometry, University of Heidelberg, Heidelberg, Germany
    PLoS ONE 6:e24247. 2011
  10. pmc Granulocyte-macrophage colony-stimulating factor-armed oncolytic measles virus is an effective therapeutic cancer vaccine
    Christian Grossardt
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, D 69120 Heidelberg, Germany
    Hum Gene Ther 24:644-54. 2013

Research Grants

  1. Vector Insertion and Mutagenesis in Human Hematopoiesis
    Christof von Kalle; Fiscal Year: 2004
  2. Vector Insertion and Mutagenesis in Human Hematopoiesis
    Christof von Kalle; Fiscal Year: 2005
  3. Vector Insertion and Mutagenesis in Human Hematopoiesis
    Christof von Kalle; Fiscal Year: 2006
  4. Vector Insertion and Mutagenesis in Human Hematopoiesis
    Christof von Kalle; Fiscal Year: 2007

Collaborators

Detail Information

Publications31

  1. pmc Real-time definition of non-randomness in the distribution of genomic events
    Ulrich Abel
    Department of Translational Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg, Germany Department of Medical Biostatistics, Tumor Center Heidelberg Mannheim, Heidelberg, Germany
    PLoS ONE 2:e570. 2007
    ..As an example, we show the effectivity and reliability of our mathematical approach in clinical retroviral vector integration site distribution...
  2. pmc Prospective, randomized, double-blind, multi-center, Phase III clinical study on transarterial chemoembolization (TACE) combined with Sorafenib versus TACE plus placebo in patients with hepatocellular cancer before liver transplantation - HeiLivCa [ISRCTN
    K Hoffmann
    Department of Surgery, Ruprecht Karls University, Heidelberg, Germany
    BMC Cancer 8:349. 2008
    ..The oral multikinase inhibitor sorafenib significantly increases overall survival and time-to-progression in patients with advanced hepatocellular cancer...
  3. doi request reprint Stable long-term blood formation by stem cells in murine steady-state hematopoiesis
    Oksana Zavidij
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany
    Stem Cells 30:1961-70. 2012
    ....
  4. pmc Insertion sites in engrafted cells cluster within a limited repertoire of genomic areas after gammaretroviral vector gene therapy
    Annette Deichmann
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center DKFZ, Heidelberg, Germany
    Mol Ther 19:2031-9. 2011
    ....
  5. pmc Gammaretrovirus-mediated correction of SCID-X1 is associated with skewed vector integration site distribution in vivo
    Kerstin Schwarzwaelder
    National Center for Tumor Diseases, German Cancer Research Center, Heidelberg, Germany
    J Clin Invest 117:2241-9. 2007
    ....
  6. pmc Extensive methylation of promoter sequences silences lentiviral transgene expression during stem cell differentiation in vivo
    Friederike Herbst
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, Heidelberg, Germany
    Mol Ther 20:1014-21. 2012
    ..Choosing the appropriate promoter is also crucial to allow stable transgene expression in clinical gene therapy...
  7. pmc Bioinformatic clonality analysis of next-generation sequencing-derived viral vector integration sites
    Anne Arens
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    Hum Gene Ther Methods 23:111-8. 2012
    ..The resulting output is summarized in tables within a convenient spreadsheet and can be further processed by researchers without profound bioinformatic knowledge...
  8. doi request reprint Comprehensive genomic access to vector integration in clinical gene therapy
    Richard Gabriel
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany
    Nat Med 15:1431-6. 2009
    ..We introduce a new nonrestrictive LAM-PCR approach that has superior capabilities for comprehensive unbiased integration site retrieval in preclinical and clinical samples independent of restriction motifs and amplification inefficiency...
  9. pmc Analyzing the number of common integration sites of viral vectors--new methods and computer programs
    Ulrich Abel
    Department of Medical Biometry, University of Heidelberg, Heidelberg, Germany
    PLoS ONE 6:e24247. 2011
    ....
  10. pmc Granulocyte-macrophage colony-stimulating factor-armed oncolytic measles virus is an effective therapeutic cancer vaccine
    Christian Grossardt
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, D 69120 Heidelberg, Germany
    Hum Gene Ther 24:644-54. 2013
    ..Thus, the treatment benefit of this combined immunovirotherapy approach has direct implications for future clinical trials...
  11. pmc Vector integration is nonrandom and clustered and influences the fate of lymphopoiesis in SCID-X1 gene therapy
    Annette Deichmann
    Institute for Molecular Medicine and Cell Research and Department of Internal Medicine I, German Cancer Research Center, University of Freiburg, Freiburg, Germany
    J Clin Invest 117:2225-32. 2007
    ..Beyond the observed cases of insertional mutagenesis in 3 patients, these data help to elucidate the relationship between vector insertion and long-term in vivo selection of transduced cells in human patients with SCID-X1...
  12. doi request reprint A largely random AAV integration profile after LPLD gene therapy
    Christine Kaeppel
    National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany
    Nat Med 19:889-91. 2013
    ..We conclude that AAV integration is potentially safe and that vector breakage and integration may occur from each position of the vector genome. Future viral integration-site analyses should include the mitochondrial genome. ..
  13. pmc Integration frequency and intermolecular recombination of rAAV vectors in non-human primate skeletal muscle and liver
    Ali Nowrouzi
    National Center for Tumor Diseases Heidelberg, Department of Translational Oncology, German Cancer Research Center, Heidelberg, Germany
    Mol Ther 20:1177-86. 2012
    ..This molecular analysis of rAAV persistence in NHP provides a promising basis for a reliable genotoxic risk assessment of rAAV in clinical trials...
  14. pmc Lentiviral vector integration profiles differ in rodent postmitotic tissues
    Cynthia C Bartholomae
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, Heidelberg, Germany
    Mol Ther 19:703-10. 2011
    ..These observations are highly relevant for the correct assessment of preclinical biosafety studies, indicating that lentiviral vectors are well suited for safe and effective clinical gene transfer into postmitotic tissues...
  15. doi request reprint Chemovirotherapy of malignant melanoma with a targeted and armed oncolytic measles virus
    Johanna K Kaufmann
    Helmholtz University Group Oncolytic Adenoviruses, German Cancer Research Center, Heidelberg, Germany
    J Invest Dermatol 133:1034-42. 2013
    ..The highly selective, entry-targeted and armed oncolytic virus MV-FCU1-αHMWMAA may become a potent building block of future melanoma therapies...
  16. pmc The fetal mouse is a sensitive genotoxicity model that exposes lentiviral-associated mutagenesis resulting in liver oncogenesis
    Ali Nowrouzi
    National Centre for Tumorigenesis, Heidelberg Technology park TP4, Heidelberg, Germany
    Mol Ther 21:324-37. 2013
    ..The fetal mouse model not only exposes the genotoxic potential of vectors intended for gene therapy but can also reveal genes associated with liver oncogenesis...
  17. pmc Retroviral vectors: post entry events and genomic alterations
    Ali Nowrouzi
    Department of Translational Oncology, German Cancer Research Center, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany
    Viruses 3:429-55. 2011
    ..We will also discuss examples of side effects occurring in ongoing human gene therapy trials and future prospectives in the field...
  18. ncbi request reprint Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing
    Anna Paruzynski
    Department of Translational Oncology, National Center for Tumor Diseases NCT, Heidelberg, Germany
    Nat Protoc 5:1379-95. 2010
    ..Thus, in combination with next-generation sequencing technologies, large-scale integrome analysis of > 4 x 10(5)-1 x 10(6) integration site sequences can be accomplished within a single week...
  19. ncbi request reprint Hematopoietic activity of human short-term repopulating cells in mobilized peripheral blood cell transplants is restricted to the first 5 months after transplantation
    Oksana Zavidij
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany
    Blood 115:5023-5. 2010
    ..This model enables to precisely determine early and late engraftment kinetics of defined human repopulating cell types and to preclinically assess the engraftment kinetics of engineered stem cell transplants...
  20. doi request reprint Detection of retroviral integration sites by linear amplification-mediated PCR and tracking of individual integration clones in different samples
    Manfred Schmidt
    Department of Translational Oncology, National Center of Tumor Diseases, Heidelberg, Germany
    Methods Mol Biol 506:363-72. 2009
    ..The recognition of the integration site sequence corresponding to a specific clone allows the tracking of an individual clone in various samples...
  21. doi request reprint Integration of retroviral vectors
    Richard Gabriel
    Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center, Im Neuenheimer Feld 460, 69120 Heidelberg, Germany
    Curr Opin Immunol 24:592-7. 2012
    ..A better knowledge of these mechanisms and interactions will allow further improving safety of retroviral vectors for gene therapy by providing an opportunity to retarget retroviral integration into non-harmful genomic positions...
  22. ncbi request reprint Targeted resequencing for analysis of clonal composition of recurrent gene mutations in chronic lymphocytic leukaemia
    Alexander Jethwa
    Department of Translational Oncology, National Centre for Tumour Diseases NCT and German Cancer Research Centre DKFZ, Heidelberg, Germany
    Br J Haematol 163:496-500. 2013
    ..We observed selection of subclones and found initial evidence for convergent mutations in CLL. Our data suggest that assessment of (sub)clonal structure may need to be integrated into analysis of the mutational profile in CLL...
  23. doi request reprint Distinct types of tumor-initiating cells form human colon cancer tumors and metastases
    Sebastian M Dieter
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, Heidelberg, Germany
    Cell Stem Cell 9:357-65. 2011
    ..We identify LT-TICs as a quantifiable target for therapies aimed toward eradication of self-renewing tumorigenic and metastatic colon cancer cells...
  24. doi request reprint An unbiased genome-wide analysis of zinc-finger nuclease specificity
    Richard Gabriel
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, Heidelberg, Germany
    Nat Biotechnol 29:816-23. 2011
    ..Comprehensive mapping of ZFN activity in vivo will facilitate the broad application of these reagents in translational research...
  25. doi request reprint Insertion site pattern: global approach by linear amplification-mediated PCR and mass sequencing
    Cynthia C Bartholomae
    Department of Translational Oncology, National Center of Tumor Diseases and German Cancer Research Center, Heidelberg, Germany
    Methods Mol Biol 859:255-65. 2012
    ..J Clin Invest 117:2225-2232, 2007). The combination of LAM-PCR and next-generation sequencing (NGS) platforms offers the opportunity to study the clonal inventory and pharmacokinetics in clinical gene therapy studies...
  26. ncbi request reprint High-resolution insertion-site analysis by linear amplification-mediated PCR (LAM-PCR)
    Manfred Schmidt
    Department of Translational Oncology, National Center for Tumor Diseases, German Cancer Research Center, Im Neuenheimer Feld 350, 69120 Heidelberg, Germany
    Nat Methods 4:1051-7. 2007
    ..LAM-PCR can be adjusted to all unknown DNA sequences adjacent to a known DNA sequence. Here we describe the use of LAM-PCR analyses to identify 5' long terminal repeat (LTR) retroviral vector adjacent genomic sequences...
  27. pmc MicroRNA-sensitive oncolytic measles viruses for cancer-specific vector tropism
    Mathias F Leber
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, Heidelberg, Germany
    Mol Ther 19:1097-106. 2011
    ..This is proof-of-concept that tropism restriction by tissue-specific microRNAs can be adapted to oncolytic MV to regulate viral replication and gene expression to maximize tumor specificity without compromising oncolytic efficacy...
  28. ncbi request reprint You can count on this: barcoded hematopoietic stem cells
    Hanno Glimm
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    Cell Stem Cell 9:390-2. 2011
    ..Recently in Nature Biotechnology, Lu et al. (2011) tagged HSCs with unique molecular barcodes and used high-throughput sequencing to track their progeny after transplantation...
  29. pmc Stable differentiation and clonality of murine long-term hematopoiesis after extended reduced-intensity selection for MGMT P140K transgene expression
    Claudia R Ball
    National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany
    Blood 110:1779-87. 2007
    ....
  30. ncbi request reprint Correction of X-linked chronic granulomatous disease by gene therapy, augmented by insertional activation of MDS1-EVI1, PRDM16 or SETBP1
    Marion G Ott
    Department of Hematology Oncology, University Hospital, German Cancer Research Center, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    Nat Med 12:401-9. 2006
    ....
  31. doi request reprint Next-generation sequencing of cancer consensus genes in lymphoma
    Jennifer Hüllein
    Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, Heidelberg, Germany
    Leuk Lymphoma 54:1831-5. 2013
    ..The application of this or similar techniques will help the development of genotype-specific treatment approaches in lymphoma...

Research Grants4

  1. Vector Insertion and Mutagenesis in Human Hematopoiesis
    Christof von Kalle; Fiscal Year: 2004
    ..Results from these studies of insertion sites in the human genome, and the role of LMO2 overexpression on the evolution of a lymphoproliferative phenotype will have great significance for the field. ..
  2. Vector Insertion and Mutagenesis in Human Hematopoiesis
    Christof von Kalle; Fiscal Year: 2005
    ..Results from these studies of insertion sites in the human genome, and the role of LMO2 overexpression on the evolution of a lymphoproliferative phenotype will have great significance for the field. ..
  3. Vector Insertion and Mutagenesis in Human Hematopoiesis
    Christof von Kalle; Fiscal Year: 2006
    ..Results from these studies of insertion sites in the human genome, and the role of LMO2 overexpression on the evolution of a lymphoproliferative phenotype will have great significance for the field. ..
  4. Vector Insertion and Mutagenesis in Human Hematopoiesis
    Christof von Kalle; Fiscal Year: 2007
    ..Results from these studies of insertion sites in the human genome, and the role of LMO2 overexpression on the evolution of a lymphoproliferative phenotype will have great significance for the field. ..