Research Topics
Genomes and Genes | U VogelSummaryCountry: Germany Publications
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Publications
Spatiotemporal analysis of invasive meningococcal disease, GermanyJohannes Elias
, , Germany
Emerg Infect Dis 12:1689-95. 2006..2%. The study exemplifies how the combination of molecular finetyping and spatiotemporal analysis can be used to assess an infectious disease in a large European country...
The use of vaccine antigen characterization, for example by MATS, to guide the introduction of meningococcus B vaccinesUlrich Vogel
Institute for Hygiene and Microbiology, National Reference Laboratory for Meningococci, University of Wurzburg, Germany
Vaccine 30:B73-7. 2012..The paper discusses the pre- and post-licensure application of MATS, the role of reference laboratories, concepts of sustained provision of the assay, external quality assessment, and laboratory twinning...- Ion torrent personal genome machine sequencing for genomic typing of Neisseria meningitidis for rapid determination of multiple layers of typing informationUlrich Vogel
Institute for Hygiene and Microbiology, University of Wurzburg, Wurzburg, Germany
J Clin Microbiol 50:1889-94. 2012..This will reduce workloads and rapidly provide information for laboratory surveillance, outbreak investigation, assessment of vaccine preventability, and antibiotic resistance gene monitoring... - European efforts to harmonize typing of meningococciUlrich Vogel
Institute for Hygiene and Microbiology, University of Wurzburg, Josef Schneider Str 2, Building E2, 97080 Wurzburg, Germany
Int J Med Microbiol 301:659-62. 2011..Although not yet feasible for routine application on hundreds of strains, novel database structures have been developed to accommodate deep sequencing data... - EpiScanGIS: an online geographic surveillance system for meningococcal diseaseMarkus Reinhardt
Computer Science II, University of Wurzburg, Germany
Int J Health Geogr 7:33. 2008..The integration of pathogen fine typing data in dynamic systems and visualization of spatio-temporal clusters are a technical challenge for system development... - Complement factor C3 deposition and serum resistance in isogenic capsule and lipooligosaccharide sialic acid mutants of serogroup B Neisseria meningitidisU Vogel
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
Infect Immun 65:4022-9. 1997.... 
Genetic lineages and their traits in Neisseria meningitidisUlrich Vogel
Institute for Hygiene and Microbiology, University of Wurzburg, Josef Schneider Strasse 2, D 97080 Wurzburg, Germany
Int J Med Microbiol 294:75-82. 2004..The oatC and oatWY genes proved to be the first genes identified to be responsible for O-acetylation of polysialic acid...- Bacteremia in an immunocompromised patient caused by a commensal Neisseria meningitidis strain harboring the capsule null locus (cnl)Ulrich Vogel
National Reference Laboratory for Meningococci, Institute for Hygiene and Microbiology, University of Wurzburg, Josef Schneider Str 2, 97080 Wurzburg, Germany
J Clin Microbiol 42:2898-901. 2004..The sequence type 845 strain was extensively typed and, furthermore, shown to be sensitive to serum bactericidal activity... - Vaccine development against Neisseria meningitidisUlrich Vogel
University of Wurzburg, Institute for Hygiene and Microbiology, Reference Laboratory for Meningococci, Germany
Microb Biotechnol 4:20-31. 2011..Research currently addresses the analysis of life vaccines, meningococcus B polysaccharide modifications and mimotopes, as well as the use of N. lactamica outer membrane vesicles... - Molecular epidemiology of Neisseria meningitidisUlrich Vogel
Institute for Hygiene and Microbiology, University of W rzburg, Germany
Front Biosci 8:e14-22. 2003.... - Meticillin-resistant Staphylococcus aureus (MRSA) contamination of ambulance cars after short term transport of MRSA-colonised patients is restricted to the stretcherS J Eibicht
University of Wurzburg, Institute for Hygiene and Microbiology, Wurzburg, Germany
J Hosp Infect 78:221-5. 2011..aureus was detected in 12 cars, predominantly at handles and headrests. This finding highlights the importance of disinfection of surfaces in the vicinity of patients irrespective of the MRSA status... - Molecular epidemiology of meningococci: application of DNA sequence typingUlrich Vogel
Institute for Hygiene and Microbiology, University of Wurzburg, Germany
Int J Med Microbiol 300:415-20. 2010..In Europe, a consensus for molecular typing has been achieved... - Functional characterization of an isogenic meningococcal alpha-2,3-sialyltransferase mutant: the role of lipooligosaccharide sialylation for serum resistance in serogroup B meningococciU Vogel
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
Med Microbiol Immunol 186:159-66. 1997.... - The evolution of human pathogens: examples and clinical implicationsU Vogel
Institut für Hygiene und Mikrobiologie, University of Wurzburg, Germany
Int J Med Microbiol 290:511-8. 2000..Furthermore, examples of the tremendous velocity of bacterial evolution under changing environmental conditions will be discussed... - Role of lipopolysaccharide sialylation in serum resistance of serogroup B and C meningococcal disease isolatesU Vogel
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, 97080 Wurzburg, Germany
Infect Immun 67:954-7. 1999..Lipopolysaccharide sialylation was found to be of minor importance for the resistance of serogroup B and C meningococcal disease isolates to complement attack... - Many carried meningococci lack the genes required for capsule synthesis and transportHeike Claus
Institute for Hygiene and Microbiology, , Josef-Schneider-Strasse 2, , Germany
Microbiology 148:1813-9. 2002.... - Differential distribution of novel restriction-modification systems in clonal lineages of Neisseria meningitidisH Claus
Institut für Hygiene und Mikrobiologie, University of Wurzburg, Wurzburg, Germany
J Bacteriol 182:1296-303. 2000.... - Nosocomial outbreak of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa associated with retrograde urographyJ Elias
Institute for Hygiene and Microbiology, University of Wuerzburg, Wuerzburg, Germany
Clin Microbiol Infect 16:1494-500. 2010..Department-specific infection control measures, including reinforced hygiene procedures during retrograde urography, quickly terminated the outbreak... - Genetic mechanisms for loss of encapsulation in polysialyltransferase-gene-positive meningococci isolated from healthy carriersMartin V R Weber
Institute for Hygiene and Microbiology, , Josef-Schneider-Strasse 2, , Germany
Int J Med Microbiol 296:475-84. 2006..These observations are consistent with the postulate that particular meningococcal clonal complexes are associated with possession of a capsule and that this association is important for transmission success... - Genetic analysis of meningococci carried by children and young adultsHeike Claus
Institute for Hygiene and Microbiology, , , Germany
J Infect Dis 191:1263-71. 2005..CONCLUSIONS: Serogroup C capsule expression during carriage may contribute to the invasive character of ST-11 complex meningococci and to the high efficacy of meningococcal serogroup C conjugate polysaccharide vaccine... - Carbohydrate composition of meningococcal lipopolysaccharide modulates the interaction of Neisseria meningitidis with human dendritic cellsOliver Kurzai
Institute of Hygiene and Microbiology, , , Germany
Cell Microbiol 7:1319-34. 2005..The use of truncated LPS isoforms in vaccine preparations can therefore not only result in attenuation but also in more efficient targeting of DC... - Genetic isolation of meningococci of the electrophoretic type 37 complexH Claus
Institute for Hygiene and Microbiology, University of Wurzburg, Wurzburg, Germany
J Bacteriol 183:2570-5. 2001.... - Mechanisms of neisserial serum resistanceU Vogel
Institut für Hygiene und Mikrobiologie, University of Wurzburg, Josef Schneider Str 2, 97080 Wurzburg, Germany
Mol Microbiol 32:1133-9. 1999..We review current concepts of neisserial serum resistance with emphasis on porins and polysaccharides exposed on the neisserial surface and their interaction with components of normal human serum... - Deletion of the meningococcal fetA gene used for antigen sequence typing of invasive and commensal isolates from Germany: frequencies and mechanismsHeike Claus
University of Wurzburg, Institute for Hygiene and Microbiology, National Reference Centre for Meningococci, Wurzburg, Germany
J Clin Microbiol 45:2960-4. 2007..ST-35 cc strains and the recently published ST-192 strains from Burkina Faso may benefit from loss of fetA, but their infrequent occurrence among invasive isolates currently does not affect fetA antigen ST... - Living in a changing environment: insights into host adaptation in Neisseria meningitidis from comparative genomicsChristoph Schoen
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Josef Schneider Str 2, Bau E1, D 97080 Wurzburg, Germany
Int J Med Microbiol 297:601-13. 2007..meningitidis an accidental pathogen. The overall properties of the meningococcal genome are characterized by genomic variability and instability, resulting in increased functional flexibility within this species... - Amino acid 310 determines the donor substrate specificity of serogroup W-135 and Y capsule polymerases of Neisseria meningitidisHeike Claus
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
Mol Microbiol 71:960-71. 2009..In silico analysis revealed a similar amino acid-based association in other members of the same glycosyltransferase family irrespective of the bacterial species... - Whole-genome comparison of disease and carriage strains provides insights into virulence evolution in Neisseria meningitidisChristoph Schoen
Institute for Hygiene and Microbiology, University of Wurzburg, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
Proc Natl Acad Sci U S A 105:3473-8. 2008..meningitidis has emerged as an unencapsulated human commensal from a common ancestor with Neisseria gonorrhoeae and Neisseria lactamica and consecutively acquired the genes responsible for capsule synthesis via horizontal gene transfer... - Genetics of capsule O-acetylation in serogroup C, W-135 and Y meningococciHeike Claus
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Josef Schneider Str 2, 97080 Wurzburg, Germany
Mol Microbiol 51:227-39. 2004..Our report is the first description of sequences of sialic acid O-acetyltransferase genes that have not been cloned from either other bacterial or mammalian organisms... - O-acetyltransferase gene neuO is segregated according to phylogenetic background and contributes to environmental desiccation resistance in Escherichia coli K1Ines L Mordhorst
Institute for Hygiene and Microbiology, University of Wurzburg, Wurzburg, Germany
Environ Microbiol 11:3154-65. 2009..In contrast, NeuO expression led to a reduced biofilm formation in biofilm positive E. coli K1 isolates. These findings suggest a delicate ecological balance of neuO'on'/'off' switching... - A dual role of extracellular DNA during biofilm formation of Neisseria meningitidisMartin Lappann
University of Wurzburg, Institute for Hygiene and Microbiology, Germany
Mol Microbiol 75:1355-71. 2010..On the contrary, spreaders (ST-11 and ST-8 cc) are unable to use eDNA for biofilm formation and might compensate for poor colonization properties by high transmission rates... - Vaccine preventability of meningococcal clone, Greater Aachen Region, GermanyJohannes Elias
University of Wuerzburg, Wuerzburg, Germany
Emerg Infect Dis 16:465-72. 2010..Because this globally distributed epidemic strain spreads slowly, vaccination efforts could possibly eliminate meningococcal disease in this area... - IS1301 fingerprint analysis of Neisseria meningitidis strains belonging to the ET-15 cloneJohannes Elias
Institute for Hygiene and Microbiology, University of Wurzburg, 97080 Wurzburg, Germany
J Clin Microbiol 45:159-67. 2007..A previously described insertion in nadA was present in more than two-thirds of the strains analyzed, suggesting that NadA is probably an unreliable vaccine candidate for the prevention of ET-15 disease... - Panton-Valentine leukocidin-positive Staphylococcus aureus infections in returning travelersDennis Tappe
Institute of Hygiene and Microbiology, University of Wurzburg, Germany
Am J Trop Med Hyg 83:748-50. 2010..Spa typing of the strains revealed a broad spectrum of variants, but some were clonally related. Methicillin-resistant Staphylococcus aureus (MRSA) was found in three cases... - Microbiological evaluation of the new VITEK 2 Neisseria-Haemophilus identification cardGiuseppe Valenza
Institute of Hygiene and Microbiology, University of Wurzburg, Josef Schneider Str 2 E1, 97080, Wurzburg, Germany
J Clin Microbiol 45:3493-7. 2007..Moreover, further reduction of the detection time would be desirable... - Molecular epidemiology of hospital-acquired vancomycin-resistant enterococciMarianne Abele-Horn
Institute for Hygiene and Microbiology, University of Wurzburg, Germany
J Clin Microbiol 44:4009-13. 2006..The combination of MLST and MLVA may be an alternative to PFGE in hospital epidemiology, providing the benefits of high accuracy, reproducibility, and portability... - Identification of a hotspot for transformation of Neisseria meningitidis by shuttle mutagenesis using signature-tagged transposonsH Claus
Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
Mol Gen Genet 259:363-71. 1998.... - Identification of the hypervirulent lineages of Neisseria meningitidis, the ST-8 and ST-11 complexes, by using monoclonal antibodies specific to NmeDIHeike Claus
Institute for Hygiene and Microbiology, , , Germany
J Clin Microbiol 41:3873-6. 2003..The gene encoding the putative restriction-modification system NmeDI is specific to these lineages. We report here a monoclonal antibody directed against the NmeDI endonuclease as a tool for their rapid and spe-cific identification... - Evaluation of custom-made DNA microarrays for multilocus sequence typing of Neisseria meningitidisHalina Swiderek
Institute for Hygiene and Microbiology, , Josef-Schneider-Str. 2, , Germany
Int J Med Microbiol 295:39-45. 2005..We assume that the high density of polymorphic sites and the extensive GC-content variations at N. meningitidis MLST loci hinder the successful implementation of MLST microarrays based on polymorphism-directed oligonucleotide design... - [Infections with Pneumococci, Menigococci, H. influenzae and Diphtheria in Germany: the RKI Reference Network for Invasive Bacterial Infections (IBI) at the 5th Würzburg Workshop on Epidemiology, Prevention and Therapy for Invasive Meningococcal Diseases T T Lam
Nationales Referenzzentrum für Meningokokken und Konsiliarlabor für H influenzae, Institut für Hygiene und Mikrobiologie, Universitat Wurzburg
Gesundheitswesen 74:747-53. 2012..pneumoniae infections. The epidemiological data were complemented by clinical aspects concerning the vaccination of immunocompromised children... - Biofilm formation by the human pathogen Neisseria meningitidisMartin Lappann
Institute for Hygiene and Microbiology, University of Wurzburg, Josef Schneider Str 2, Building E1, Wurzburg, Germany
Med Microbiol Immunol 199:173-83. 2010..The theory of sub-dividing the meningococcal population in settler and spreader lineages is discussed, which provides a mechanistic framework for the assumed balance of colonization efficacy and transmission frequency... - Streptococcus merionis sp. nov., isolated from Mongolian jirds (Meriones unguiculatus)Dennis Tappe
Institute of Hygiene and Microbiology, University of Wurzburg, Josef Schneider Strasse 2, D 97080 Wurzburg, Germany
Int J Syst Evol Microbiol 59:766-70. 2009..nov. is proposed. The type strain is WUE3771(T) (=DSM 19192(T)=CCUG 54871(T))... - Meningococcal biofilm formation: structure, development and phenotypes in a standardized continuous flow systemMartin Lappann
, Institute for Hygiene and Microbiology, Germany
Mol Microbiol 62:1292-309. 2006..PilX alleles differed in their propensity to support autoaggregation of cells in suspension, but not in their ability to support microcolony formation within biofilms in the continuous flow system... - Microarray analyses of meningococcal genome composition and gene regulation: a review of the recent literatureHeike Claus
Institute for Hygiene and Microbiology, University of Wurzburg, Wurzburg, Germany
FEMS Microbiol Rev 31:43-51. 2007..However, harmonization of protocols and tools, as well as central databases are needed to foster the comparability of studies and the integration of knowledge... - Evidence for indirect nosocomial transmission of Neisseria meningitidis resulting in two cases of invasive meningococcal diseaseJohannes Elias
National Reference Centre for Meningococci, Institute for Hygiene and Microbiology, , Germany
J Clin Microbiol 44:4276-8. 2006..The most likely epidemiological link was simultaneous hospitalization, resulting in indirect nosocomial transmission... - Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogensGerhard Hutter
Institute for Hygiene, , Germany
Microbiology 149:67-75. 2003.... - Microbial changes in periodontitis successfully treated by mechanical plaque removal and systemic amoxicillin and metronidazoleGiuseppe Valenza
Institute for Hygiene and Microbiology, University of Wurzburg, Josef Schneider Str 2 E1, 97080 Wurzburg, Germany
Int J Med Microbiol 299:427-38. 2009..In summary, despite a favorable clinical outcome, a transient effect on only few bacterial species was observed... - Analysis of the periodontal microbiota in childhood-type hypophosphatasiaGiuseppe Valenza
Institute for Hygiene and Microbiology, , Josef-Schneider-Strasse 2/E1, , Germany
Int J Med Microbiol 296:493-500. 2006.... - The meningococcal vaccine candidate GNA1870 binds the complement regulatory protein factor H and enhances serum resistanceGuillermo Madico
Section of Infectious Diseases, Boston University School of Medicine, Boston, MA 02118, USA
J Immunol 177:501-10. 2006..These data provide further support for inclusion of this molecule in a meningococcal vaccine. To reflect the critical function of this molecule, we suggest calling it fH-binding protein... - Interlaboratory comparison of PCR-based identification and genogrouping of Neisseria meningitidisMuhamed Kheir Taha
Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France
J Clin Microbiol 43:144-9. 2005..Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs... - O-Acetylation status of the capsular polysaccharides of serogroup Y and W135 meningococci isolated in the UKEmma Longworth
PHLS Meningococcal Reference Unit, Manchester Public Health Laborotory, Withington Hospital, Manchester M20 2LR, UK
FEMS Immunol Med Microbiol 32:119-23. 2002..Although the biological relevance of O-acetylation status is unknown for these serogroups, an understanding of O-acetylation status of the respective polysaccharides may provide useful insights into the optimal vaccine formulation... 
Biochemical characterization of a Neisseria meningitidis polysialyltransferase reveals novel functional motifs in bacterial sialyltransferasesFriedrich Freiberger
Abteilung Zelluläre Chemie, Medizinische Hochschule Hannover, Carl Neuberg Str 1, 30625 Hannover, Germany
Mol Microbiol 65:1258-75. 2007....- Standardized nonculture techniques recommended for European reference laboratoriesAndrew J Fox
Institute for Hygiene and Mikrobiologie, National Reference Centre for Meningococci, Wurzburg, Germany
FEMS Microbiol Rev 31:84-8. 2007..These approaches can be implemented either by individual reference laboratories or through collaboration and referral between centres... - Three cases of invasive meningococcal disease caused by a capsule null locus strain circulating among healthy carriers in Burkina FasoHelen Findlow
Vaccine Evaluation Unit, Health Protection Agency North West, Manchester Royal Infirmary, Manchester, M13 9WZ, UK
J Infect Dis 195:1071-7. 2007..The presence of cnl meningococci that can escape serum killing and cause invasive disease is of concern for future vaccination strategies and should promote rigorous surveillance of cnl meningococcal disease... - Biochemical characterization of the polysialic acid-specific O-acetyltransferase NeuO of Escherichia coli K1Anne K Bergfeld
Department of Cellular Chemistry, Medical School Hannover, Carl Neuberg Strasse 1, Hannover, Germany
J Biol Chem 282:22217-27. 2007.... - Workshop on vaccine pressure and Neisseria meningitidis, Annecy, France, 9-11 March 2005Jean Michel Alonso
Institut Pasteur, Paris, France
Vaccine 25:4125-9. 2007..The workshop concluded that there is no current evidence to suggest that serogroup C conjugate vaccine pressure has resulted in meningococcal serogroup switching or replacement... - Target gene sequencing to characterize the penicillin G susceptibility of Neisseria meningitidisMuhamed Kheir Taha
Neisseria Unit, Institut Pasteur, 28 rue du Dr Roux, Paris, France
Antimicrob Agents Chemother 51:2784-92. 2007..These data argue for the use of penA sequencing to identify isolates with reduced susceptibility to penicillin G and as a tool to improve typing of meningococcal isolates, as well as to analyze DNA exchange among Neisseria species... - Factor H binding and function in sialylated pathogenic neisseriae is influenced by gonococcal, but not meningococcal, porinGuillermo Madico
Evans Biomedical Research Center, Boston University Medical Center, Boston, MA 02118, USA
J Immunol 178:4489-97. 2007..Our data highlight the different mechanisms used by these two related species to evade complement... - Identification of osteopontin as the most consistently over-expressed gene in intrahepatic cholangiocarcinoma: detection by oligonucleotide microarray and real-time PCR analysisHolger G Hass
Department of Oncology, Hematology and Palliative Care, Marien Hospital, Boeheim Street 37, Stuttgart 70199, Germany
World J Gastroenterol 14:2501-10. 2008..To investigate the molecular pathways involved in human cholangiocarcinogenesis by gene expression profiling... - Defining targets for complement components C4b and C3b on the pathogenic neisseriaeLisa A Lewis
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Lazare Research Building, Room 370I, 364 Plantation Street, Worcester, MA 01605, USA
Infect Immun 76:339-50. 2008.... - Neisserial lipooligosaccharide is a target for complement component C4b. Inner core phosphoethanolamine residues define C4b linkage specificitySanjay Ram
Section of Infectious Diseases, Evans Biomedical Research Center, Boston University Medical Center, Boston, Massachusetts 02118, USA
J Biol Chem 278:50853-62. 2003.... - Evolution of bacteriophages infecting encapsulated bacteria: lessons from Escherichia coli K1-specific phagesKatharina Stummeyer
Abteilung Zelluläre Chemie, Zentrum Biochemie, Medizinische Hochschule Hannover, Carl Neuberg Street 1, 30625 Hannover, Germany
Mol Microbiol 60:1123-35. 2006..Thereby, the present study contributes not only to an improved understanding of phage evolution and host-range extension but may also facilitate the on purpose design of therapeutic phages based on well-characterized template phages... - DNA sequence-based tandem repeat analysis of the clfB gene is less discriminatory than spa typing for methicillin-resistant Staphylococcus aureusDag Harmsen
Institute for Hygiene, , , Germany
Int J Med Microbiol 294:525-8. 2005..Using epidemic MRSA isolates from two German university hospitals, we show that the clfB repeat region does not offer any additional discriminatory power... - Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database managementDag Harmsen
Institute for Hygiene, , , Germany
J Clin Microbiol 41:5442-8. 2003..spa typing was valuable for tracking of epidemic isolates. The data show that disproval of epidemiologically suggested transmissions of MRSA is one of the main objectives of spa typing in departments with a high incidence of MRSA...