Ulrike Holzgrabe

Summary

Affiliation: Urologische Klinik und Poliklinik sowie Medizinische Klinik
Country: Germany

Publications

  1. ncbi request reprint [Perfect drug targeting. Glufosfamide a new derivative]
    Ulrike Holzgrabe
    Institut f Pharmazie und LMC Universität Würzburg
    Pharm Unserer Zeit 35:158-9. 2006
  2. ncbi request reprint Analytical characterisation of crude extracts from an African Ancistrocladus species using high-performance liquid chromatography and capillary electrophoresis coupled to ion trap mass spectrometry
    Matthias Unger
    Institute of Pharmacy and Food Chemistry, Julius Maximilians University Würzburg, D 97074 Wurzburg, Germany
    Phytochem Anal 15:21-6. 2004
  3. ncbi request reprint Capillary zone electrophoresis as a tool for the quality control of goldenseal extracts
    Matthias Unger
    Institute of Pharmacy and Food Chemistry, Julius Maximilians University, Wurzburg, Germany
    Electrophoresis 26:2430-6. 2005
  4. ncbi request reprint Random chemistry as a new tool for the generation of small compound libraries: development of a new acetylcholinesterase inhibitor
    Petra Kapkova
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    J Med Chem 48:7496-9. 2005
  5. ncbi request reprint Interlaboratory study of a NACE method for the determination of R-timolol content in S-timolol maleate: assessment of uncertainty
    Roland Djang eing a Marini
    Laboratory of Analytical Chemistry, Institute of Pharmacy, University of Liege, Liege, Belgium
    Electrophoresis 27:2386-99. 2006
  6. ncbi request reprint Why not using capillary electrophoresis in drug analysis?
    Ulrike Holzgrabe
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 27:2283-92. 2006
  7. ncbi request reprint Quantitative liquid chromatography-mass spectrometry determination of isatin in urine using automated on-line extraction
    Matthias Unger
    Department of Biopharmaceutical Sciences, University of California, San Francisco 94143 0446, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 767:245-53. 2002
  8. ncbi request reprint Impurity profiling of atropine sulfate by microemulsion electrokinetic chromatography
    Yaser Bitar
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    J Pharm Biomed Anal 44:623-33. 2007
  9. ncbi request reprint Targeting acetylcholinesterase to treat neurodegeneration
    Ulrike Holzgrabe
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    Expert Opin Ther Targets 11:161-79. 2007
  10. ncbi request reprint Amino acids: aspects of impurity profiling by means of CE
    Susanne Kopec
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 28:2153-67. 2007

Collaborators

  • Matthias Unger
  • Gerhard Bringmann
  • Serge Rudaz
  • Roberto Gotti
  • Yaser Bitar
  • Verena Hoerr
  • Daniela Brinz
  • Nikolai Novatchev
  • Cornelia B Landersdorfer
  • Susanne Kopec
  • George L Drusano
  • Martina Kinzig-Schippers
  • Roland Djang eing a Marini
  • Fritz Sorgel
  • Petra Kapkova
  • Wolfgang A Krueger
  • Ulrich Schmitt
  • Ralph Deubner
  • Frank Wienen
  • Bilal Awadallah
  • Stefanie Laug
  • Maike Wedig
  • Jurgen B Bulitta
  • Wilma Ziebuhr
  • Svetlana Kozitskaya
  • Elena Katzowitsch
  • Carl M J Kirkpatrick
  • Pham Thi Thanh Ha
  • Julie Schappler
  • Jean Luc Veuthey
  • Jie Zhang
  • Attilio Ceccato
  • Jos Hoogmartens
  • Jalal Hawari
  • Govert W Somsen
  • Marianne Fillet
  • Willy Van Ael
  • Debby Mangelings
  • Yvan Vander Heyden
  • Matteo Pedrini
  • Gerhardus J de Jong
  • Bruno Boulanger
  • Roelof Mol
  • Jacques Crommen
  • Anne Catherine Servais
  • Ann Van Schepdael
  • Ilias Jimidar
  • Carl Groom
  • Philippe Hubert
  • Eric Rozet
  • François R Doucet
  • Willy Brione
  • Cornelia Landersdorfer
  • Kurt G Naber
  • Tanja Schirmeister
  • Jurgen Bulitta
  • Björn Degel
  • Gerd Folkers
  • Eberhard Heller
  • Mevlut Ertan
  • August Stich
  • Knut Baumann
  • Albrecht Schwab
  • Peter C Schmidt
  • Martin A Wahl
  • Stefan Just
  • Tina Worch

Detail Information

Publications27

  1. ncbi request reprint [Perfect drug targeting. Glufosfamide a new derivative]
    Ulrike Holzgrabe
    Institut f Pharmazie und LMC Universität Würzburg
    Pharm Unserer Zeit 35:158-9. 2006
  2. ncbi request reprint Analytical characterisation of crude extracts from an African Ancistrocladus species using high-performance liquid chromatography and capillary electrophoresis coupled to ion trap mass spectrometry
    Matthias Unger
    Institute of Pharmacy and Food Chemistry, Julius Maximilians University Würzburg, D 97074 Wurzburg, Germany
    Phytochem Anal 15:21-6. 2004
    ..By performing additional CE-MS/MS experiments, in combination with parallel isolation studies and structural elucidation using conventional methods, six alkaloidal substances present in the plant could be identified...
  3. ncbi request reprint Capillary zone electrophoresis as a tool for the quality control of goldenseal extracts
    Matthias Unger
    Institute of Pharmacy and Food Chemistry, Julius Maximilians University, Wurzburg, Germany
    Electrophoresis 26:2430-6. 2005
    ..0 microg/mL and the linearity was obtained between 2.5 and 500 microg/mL. Using our newly developed method, both the alkaloids could be analysed in herbal remedies containing goldenseal root extracts within 8 min...
  4. ncbi request reprint Random chemistry as a new tool for the generation of small compound libraries: development of a new acetylcholinesterase inhibitor
    Petra Kapkova
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    J Med Chem 48:7496-9. 2005
    ..The structure of the new highly potent AChE inhibitor, 9-amino-5,6,7,8-tetrahydroacridin-4yl)methanol (1), was elucidated by NMR spectroscopy and ESI (tandem) mass spectrometry...
  5. ncbi request reprint Interlaboratory study of a NACE method for the determination of R-timolol content in S-timolol maleate: assessment of uncertainty
    Roland Djang eing a Marini
    Laboratory of Analytical Chemistry, Institute of Pharmacy, University of Liege, Liege, Belgium
    Electrophoresis 27:2386-99. 2006
    ..The estimated uncertainty, derived from the precision estimates, seems to be concentration-dependent above a given threshold. This example of R-timolol illustrates how a laboratory can evaluate uncertainty in general...
  6. ncbi request reprint Why not using capillary electrophoresis in drug analysis?
    Ulrike Holzgrabe
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 27:2283-92. 2006
    ..Taken together CE should be more often applied in drug quality control...
  7. ncbi request reprint Quantitative liquid chromatography-mass spectrometry determination of isatin in urine using automated on-line extraction
    Matthias Unger
    Department of Biopharmaceutical Sciences, University of California, San Francisco 94143 0446, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 767:245-53. 2002
    ..The assay was linear from 5 to 5000 ng/ml (r2>0.99) and analytical recovery was >80%. Inter-assay precision for the quality control samples was less than 3% and inter-assay accuracy was within +/- 5%...
  8. ncbi request reprint Impurity profiling of atropine sulfate by microemulsion electrokinetic chromatography
    Yaser Bitar
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    J Pharm Biomed Anal 44:623-33. 2007
    ..Good agreement was obtained between the established MEEKC method and the traditional RP-HPLC method...
  9. ncbi request reprint Targeting acetylcholinesterase to treat neurodegeneration
    Ulrike Holzgrabe
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    Expert Opin Ther Targets 11:161-79. 2007
    ..This dual action might be more beneficial for treatment of Alzheimer s disease than simple inhibition of the acetylcholine hydrolysis. Thus, the new bivalent ligands are the focus of this review...
  10. ncbi request reprint Amino acids: aspects of impurity profiling by means of CE
    Susanne Kopec
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 28:2153-67. 2007
    ..7 and amino acid samples after derivatization with 9-fluorenylmethyl chloroformate (FMOC) and 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) may pave the way for impurity profiling of fermentatively produced API by means of CE...
  11. pmc Population pharmacokinetics at two dose levels and pharmacodynamic profiling of flucloxacillin
    Cornelia B Landersdorfer
    Institute for Biomedical and Pharmaceutical Research, Nürnberg Heroldsberg, Germany
    Antimicrob Agents Chemother 51:3290-7. 2007
    ..Prolonged infusion and continuous infusion are appealing options for the treatment of serious infections caused by sensitive staphylococci...
  12. ncbi request reprint Enantioseparation of chiral tropa alkaloids by means of cyclodextrin-modified microemulsion electrokinetic chromatography
    Yaser Bitar
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 28:2693-700. 2007
    ....
  13. ncbi request reprint Laser-induced fluorescence-capillary electrophoresis and fluorescence microplate reader measurement: two methods to quantify the effect of antibiotics
    Verena Hoerr
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Anal Chem 79:7510-8. 2007
    ..Thus, the tendency of bacterial chain formation and alterations in the live/dead ratio of the bacterial composition can be directly observed in the presence of different antibiotics...
  14. ncbi request reprint Development and validation of a separation method for the diastereomers and enantiomers of aziridine-type protease inhibitors
    Yaser Bitar
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 26:2313-9. 2005
    ..The corrected peak-area ratios, migration times, and resolutions were found to be robust with respect to small variations of voltage, buffer concentrations, pH, temperature, chiral selector concentration, and different lots...
  15. pmc Evaluation by monte carlo simulation of the pharmacokinetics of two doses of meropenem administered intermittently or as a continuous infusion in healthy volunteers
    Wolfgang A Krueger
    IBMP Institute for Biomedical and Pharmaceutical Research, Paul Ehrlich Str 19, D 90562 Nürnberg Heroldsberg, Germany
    Antimicrob Agents Chemother 49:1881-9. 2005
    ..We conclude that clinical trials for evaluation of the continuous infusions of meropenem in critically ill patients are warranted...
  16. ncbi request reprint The use of cyclodextrin-modified capillary electrophoresis in the determination of the enantiomeric purity
    Ulrike Holzgrabe
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Germany
    J Capill Electrophor Microchip Technol 7:23-9. 2002
    ..The poor peak shape found with anionic CDs could be improved by addition of methanol...
  17. ncbi request reprint Evaluation of amino sugar, low molecular peptide and amino acid impurities of biotechnologically produced amino acids by means of CE
    Nikolai Novatchev
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074, Wurzburg, Germany
    J Pharm Biomed Anal 28:475-86. 2002
    ..1%. Confidence intervals below +/-2.5% (lambda=0.05, n=9) of the target were found sufficient for purity tests...
  18. ncbi request reprint Evaluation of the impurity profile of alcuronium by means of capillary electrophoresis
    Maike Wedig
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074, Wurzburg, Germany
    J Pharm Biomed Anal 28:983-90. 2002
    ..In addition, the conversion of alcuronium to DAC, occurring under acidic condition, was monitored by means of the CE method developed...
  19. ncbi request reprint Determination of clotrimazole in mice plasma by capillary electrophoresis
    Frank Wienen
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, Würzburg 97074, Germany
    J Pharm Biomed Anal 30:1879-87. 2003
    ..3 microg ml(-1) (limit of detection), and to quantify clotrimazole down to concentrations of 0.5 microg ml(-1) (limit of quantification)...
  20. ncbi request reprint Inhibition of cytochrome P450 3A4 by extracts and kavalactones of Piper methysticum (Kava-Kava)
    Matthias Unger
    Institute of Pharmacy and Food Chemistry, Julius Maximilians University, Wurzburg, Germany
    Planta Med 68:1055-8. 2002
    ..Positive fractions were analysed with LC/MS using electrospray ionisation and kavapyrones could be identified as the main CYP3A4 inhibitory components of Piper methysticum...
  21. ncbi request reprint Quantitation of talinolol and other beta-blockers by capillary electrophoresis for in vitro drug absorption studies
    Bilal Awadallah
    Department of Pharmaceutical Technology, Eberhard Karls University of Tubingen, Auf der Morgenstelle 8, D 72076 Tubingen, Germany
    Electrophoresis 24:2627-32. 2003
    ..For determining talinolol, the method could be validated in terms of precision, accuracy and linearity, and was found to be suitable in determination of talinolol enantiomers in highly diluted samples obtained from in vitro experiments...
  22. ncbi request reprint Micellar electrokinetic capillary chromatography, high performance liquid chromatography and nuclear magnetic resonance--three orthogonal methods for characterization of critical drugs
    Ralph Deubner
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    J Pharm Biomed Anal 35:459-67. 2004
    ..In this study NMR spectroscopy was found to be a very good orthogonal and complementary method...
  23. ncbi request reprint Chiral capillary electrophoresis: facts and fiction on the reproducibility of resolution with randomly substituted cyclodextrins
    Ulrich Schmitt
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 25:2801-7. 2004
    ..Taken together, a validation of a chiral separation with "undefined" CD derivatives is almost impossible...
  24. ncbi request reprint Critical aspects of analysis of Micrococcus luteus, Neisseria cinerea, and Pseudomonas fluorescens by means of capillary electrophoresis
    Verena Hoerr
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 25:3132-8. 2004
    ..luteus) and Gram-negative (N. cinerea) cocci and on the other hand Gram-negative, rod-shaped bacteria (P. fluorescens), are probably responsible for the different pretreatment conditions...
  25. ncbi request reprint [A riddle solved--why valerian-hops extract makes you drowsy]
    Ulrike Holzgrabe
    Pharm Unserer Zeit 33:348. 2004
  26. ncbi request reprint Analysis of the glucosinolate pattern of Arabidopsis thaliana seeds by capillary zone electrophoresis coupled to electrospray ionization-mass spectrometry
    Gerhard Bringmann
    Institute of Organic Chemistry, University of Wurzburg, Wurzburg, Germany
    Electrophoresis 26:1513-22. 2005
    ..The sensitivity together with mass accuracy and true isotopic pattern of the TOF-MS allowed identification of a broad series of glucosinolates in Arabidopsis thaliana seeds...
  27. doi request reprint Analysis of the molecular size distribution of polyethylene glycols by CE-UV versus HPLC with evaporative light scattering detection
    Daniela Brinz
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, Wurzburg, Germany
    Electrophoresis 29:3605-11. 2008
    ..This method was successfully applied to the analysis of underivatized PEG 400, 1000 and 1500...