Roland Zengerle

Summary

Affiliation: University of Freiburg
Country: Germany

Publications

  1. doi request reprint Optical non-contact localization of liquid-gas interfaces on disk during rotation for measuring flow rates and viscosities
    Jochen Hoffmann
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, Freiburg, 79110, Germany
    Lab Chip 12:5231-6. 2012
  2. pmc Cyclic electrodeposition of PtCu alloy: facile fabrication of highly porous platinum electrodes
    Arne Kloke
    Laboratory for MEMS Applications, IMTEK Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany
    Adv Mater 24:2916-21. 2012
  3. doi request reprint Microstructuring of polymer films for sensitive genotyping by real-time PCR on a centrifugal microfluidic platform
    Maximilian Focke
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, 79110 Freiburg, Germany
    Lab Chip 10:2519-26. 2010
  4. doi request reprint Lab-on-a-Foil: microfluidics on thin and flexible films
    Maximilian Focke
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, 79110 Freiburg, Germany
    Lab Chip 10:1365-86. 2010
  5. doi request reprint Solid-phase PCR in a picowell array for immobilizing and arraying 100,000 PCR products to a microscope slide
    Jochen Hoffmann
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, Freiburg, 79110, Germany
    Lab Chip 12:3049-54. 2012
  6. doi request reprint Strategies to extend the lifetime of bioelectrochemical enzyme electrodes for biosensing and biofuel cell applications
    Stefanie Rubenwolf
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, 79110 Freiburg, Germany
    Appl Microbiol Biotechnol 89:1315-22. 2011
  7. doi request reprint Mediator Probe PCR: A Novel Approach for Detection of Real-Time PCR Based on Label-Free Primary Probes and Standardized Secondary Universal Fluorogenic Reporters
    Bernd Faltin
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Freiburg, Germany
    Clin Chem 58:1546-56. 2012
  8. doi request reprint Centrifugal gas-phase transition magnetophoresis (GTM)--a generic method for automation of magnetic bead based assays on the centrifugal microfluidic platform and application to DNA purification
    Oliver Strohmeier
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, 79110 Freiburg, Germany
    Lab Chip 13:146-55. 2013
  9. ncbi request reprint Strategies for the fabrication of porous platinum electrodes
    Arne Kloke
    Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, 79110 Freiburg, Germany
    Adv Mater 23:4976-5008. 2011
  10. ncbi request reprint Highly parallel dispensing of chemical and biological reagents
    Bas De Heij
    IMTEK, University of Freiburg, Georges KoehlerAllee 103, 79110 Freiburg, Germany
    Anal Bioanal Chem 378:119-22. 2004

Collaborators

  • Daniel Mark
  • Oliver Gutmann
  • Stefanie Rubenwolf
  • Jacques Schrenzel
  • Artur Tropmann
  • Sascha Lutz
  • Patrice Francois
  • Stefan Haeberle
  • Juergen Steigert
  • Felix von Stetten
  • Günter Roth
  • Jochen Hoffmann
  • Maximilian Focke
  • Arne Kloke
  • Bernd Faltin
  • Oliver Strohmeier
  • Nils Paust
  • Steffen Zehnle
  • Andreas Ernst
  • Sven Kerzenmacher
  • Simon Wadle
  • Fabian Stumpf
  • Holger Reinecke
  • Claas Müller
  • Peter Koltay
  • J D Jeyaprakash S Samuel
  • Bas De Heij
  • Alexander Emperle
  • Martin Trotter
  • Laurent Tanguy
  • Frank Schwemmer
  • Klaus Mutschler
  • Jens Ducree
  • Lutz Riegger
  • Christian Kohler
  • Frederik Bundgaard
  • Ramona Gerwig
  • Dylan Bamarni
  • Patrick Reith
  • Dominique Kosse
  • Reinhard Steger
  • Gerhard Birkle
  • Jürgen Rühe
  • Remigius Niekrawietz
  • Martina Daub
  • Hermann Sandmaier

Detail Information

Publications23

  1. doi request reprint Optical non-contact localization of liquid-gas interfaces on disk during rotation for measuring flow rates and viscosities
    Jochen Hoffmann
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, Freiburg, 79110, Germany
    Lab Chip 12:5231-6. 2012
    ..9%. Additionally, flow rates and viscosities (range: 1-12 mPa s, precision of 4.3%) can be sensed even during rotation at frequencies up to 30 Hz...
  2. pmc Cyclic electrodeposition of PtCu alloy: facile fabrication of highly porous platinum electrodes
    Arne Kloke
    Laboratory for MEMS Applications, IMTEK Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany
    Adv Mater 24:2916-21. 2012
    ..The underlying processes, resulting electrode structures, and their applicability to potentially implantable glucose fuel cells are discussed...
  3. doi request reprint Microstructuring of polymer films for sensitive genotyping by real-time PCR on a centrifugal microfluidic platform
    Maximilian Focke
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, 79110 Freiburg, Germany
    Lab Chip 10:2519-26. 2010
    ..The samples contained clinical samples of seven different genotypes of MRSA as well as positive and negative controls. The results are in excellent agreement with the reference in microtubes...
  4. doi request reprint Lab-on-a-Foil: microfluidics on thin and flexible films
    Maximilian Focke
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, 79110 Freiburg, Germany
    Lab Chip 10:1365-86. 2010
    ..We conclude that the Lab-on-a-Foil approach is very versatile and significantly expands the toolbox for the development of Lab-on-a-Chip solutions...
  5. doi request reprint Solid-phase PCR in a picowell array for immobilizing and arraying 100,000 PCR products to a microscope slide
    Jochen Hoffmann
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, Freiburg, 79110, Germany
    Lab Chip 12:3049-54. 2012
    ..This enables the subsequent use for massively parallel analysis similar to standard microarrays...
  6. doi request reprint Strategies to extend the lifetime of bioelectrochemical enzyme electrodes for biosensing and biofuel cell applications
    Stefanie Rubenwolf
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, 79110 Freiburg, Germany
    Appl Microbiol Biotechnol 89:1315-22. 2011
    ..Alternatively, integrated microorganisms can display the enzymes on their surface or secrete them to the electrolyte, allowing unattended power supply for long-term applications...
  7. doi request reprint Mediator Probe PCR: A Novel Approach for Detection of Real-Time PCR Based on Label-Free Primary Probes and Standardized Secondary Universal Fluorogenic Reporters
    Bernd Faltin
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Freiburg, Germany
    Clin Chem 58:1546-56. 2012
    ..Sequence-dependent universal detection techniques overcome this drawback but are prone to detection of unspecific amplification products. We developed the mediator probe PCR as a solution to these problems...
  8. doi request reprint Centrifugal gas-phase transition magnetophoresis (GTM)--a generic method for automation of magnetic bead based assays on the centrifugal microfluidic platform and application to DNA purification
    Oliver Strohmeier
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, 79110 Freiburg, Germany
    Lab Chip 13:146-55. 2013
    ..g. to immunoassays or protein affinity purification, solely requiring the adjustment of number and volumes of the fluidic chambers...
  9. ncbi request reprint Strategies for the fabrication of porous platinum electrodes
    Arne Kloke
    Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, 79110 Freiburg, Germany
    Adv Mater 23:4976-5008. 2011
    ..This way, fabrication methods not performable with platinum can be applied to the fabrication of platinum electrodes with the special benefit of low platinum consumption...
  10. ncbi request reprint Highly parallel dispensing of chemical and biological reagents
    Bas De Heij
    IMTEK, University of Freiburg, Georges KoehlerAllee 103, 79110 Freiburg, Germany
    Anal Bioanal Chem 378:119-22. 2004
    ..Based on our standard configuration we achieved droplets with 125- micro m in-flight diameter (1.2 nL) with a CV of <1%...
  11. ncbi request reprint A highly parallel nanoliter dispenser for microarray fabrication
    Oliver Gutmann
    IMTEK University of Freiburg, Laboratory for MEMS Applications, Georges Koehler Allee 103, D 79110 Freiburg, Germany
    Biomed Microdevices 6:131-7. 2004
    ..With it a full DNA hybridization experiment was performed. Clear positive signals one hand and no signals in the negative controls on the other hand showed that our system is suited for microarray production...
  12. doi request reprint A modular diffusion barrier based on phase separation for localized delivery of discrete drug volumes in aqueous environments
    Juergen Steigert
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, Albert Ludwigs University of Freiburg, Georges Koehler Allee 106, D 79110, Freiburg, Germany
    Lab Chip 9:1801-5. 2009
    ..For the phase-gap we have proven an excellent long-term stability of more than 30 hours against capillary priming...
  13. ncbi request reprint Centrifugal extraction of plasma from whole blood on a rotating disk
    Stefan Haeberle
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, D 79110 Freiburg, Germany
    Lab Chip 6:776-81. 2006
    ..The residual cell concentration in the purified plasma amounts to less than 0.11%, independent of the frequency of rotation. A capillary duct connects the extracted plasma to subsequent on-disk processing units...
  14. ncbi request reprint Fast and reliable protein microarray production by a new drop-in-drop technique
    Oliver Gutmann
    IMTEK University of Freiburg, Laboratory for MEMS Applications, Georges Koehler Allee 103, D 79110 Freiburg, Germany
    Lab Chip 5:675-81. 2005
    ..Additionally the very fast UV cross-linking was used to immobilize antibodies. The optimized system was used to produce antibody microarrays and with it microarray ELISA experiments were performed successfully...
  15. ncbi request reprint Non-contact production of oligonucleotide microarrays using the highly integrated TopSpot nanoliter dispenser
    Oliver Gutmann
    IMTEK University of Freiburg, Georges Koehler Allee 103, D 79110 Freiburg, Germany
    Analyst 129:835-40. 2004
    ..Furthermore, buffers and protocols were adapted to a host of different microarray slides used. With this system, prime critical points of microarray production are solved, leading to high quality high throughput microarray fabrication...
  16. doi request reprint Centrifugal microfluidic system for primary amplification and secondary real-time PCR
    Maximilian Focke
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, 79110, Freiburg, Germany
    Lab Chip 10:3210-2. 2010
    ....
  17. doi request reprint Microfluidic Apps for off-the-shelf instruments
    Daniel Mark
    HSG IMIT Institut für Mikro und Informationstechnik, Georges Koehler Allee 103, 79110 Freiburg, Germany
    Lab Chip 12:2464-8. 2012
    ..Microfluidic Apps could be considered as low-cost disposables which can easily be distributed globally via web-shops. Therefore they could be a door-opener for high-volume mass markets...
  18. doi request reprint Centrifugo-dynamic inward pumping of liquids on a centrifugal microfluidic platform
    Steffen Zehnle
    HSG IMIT Institut für Mikro und Informationstechnik, Georges Koehler Allee 103, 79110 Freiburg, Germany
    Lab Chip 12:5142-5. 2012
    ..By employing three pump cycles, water has been pumped radially inwards with an efficiency of >90%. The inward pumping requires centrifugation only, which is intrinsically available on every centrifugal microfluidic platform...
  19. pmc Numerical investigations on electric field characteristics with respect to capacitive detection of free-flying droplets
    Andreas Ernst
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, 79110 Freiburg, Germany
    Sensors (Basel) 12:10550-65. 2012
    ..The sensitivity of the focused capacitor geometry was evaluated to be S(i) = 0.3 fC/nL. The simulation results are validated by experiments which exhibit good agreement...
  20. doi request reprint Completely superhydrophobic PDMS surfaces for microfluidics
    Artur Tropmann
    Laboratory for MEMS Applications, IMTEK Department of Microsystems Engineering, University of Freiburg, Georges Koehler Allee 103, Freiburg im Breisgau, Germany 79110
    Langmuir 28:8292-5. 2012
    ..The best superhydrophobicity is observed for samples with a PTFE particle concentration of 15 wt %, which have advancing and receding contact angles of 159° ± 4° and 158° ± 3°, respectively...
  21. doi request reprint Carbon electrodes for direct electron transfer type laccase cathodes investigated by current density-cathode potential behavior
    Stefanie Rubenwolf
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 103, 79110 Freiburg, Germany
    Biosens Bioelectron 26:841-5. 2010
    ..In case of the latter, an improved dispersion of the nanotubes upon electrode fabrication may greatly enhance their performance...
  22. doi request reprint Pre-storage of liquid reagents in glass ampoules for DNA extraction on a fully integrated lab-on-a-chip cartridge
    Jochen Hoffmann
    Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges Koehler Allee 106, 79110 Freiburg, Germany
    Lab Chip 10:1480-4. 2010
    ..The presented approach allows the improvement of existing LoaC cartridges where pre-storage of liquid reagents was not implemented yet...
  23. ncbi request reprint Modification of micronozzle surfaces using fluorinated polymeric nanofilms for enhanced dispensing of polar and nonpolar fluids
    J D Jeyaprakash S Samuel
    Laboratory for MEMS Applications, Institute for Microsystems Technology, University of Freiburg, 79110 Freiburg, Germany
    Anal Chem 77:6469-74. 2005
    ..The modified nozzles have been tested with a variety of fluids having a wide range of surface tensions. The surface modification allows precise control of the dispensing of nanoliter droplets with a high degree of reproducibility...