Ralf Weiskirchen

Summary

Affiliation: University Hospital
Country: Germany

Publications

  1. ncbi request reprint Analysis of the transforming growth factor-beta1 (TGF-beta1) codon 25 gene polymorphism by LightCycler-analysis in patients with chronic hepatitis C infection
    Carmen G Tag
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Pauwelsstr 30, D 52074, Aachen, Germany
    Cytokine 24:173-81. 2003
  2. ncbi request reprint TGF-beta up-regulates serum response factor in activated hepatic stellate cells
    Jens Herrmann
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, D 52074, Germany
    Biochim Biophys Acta 1772:1250-7. 2007
  3. ncbi request reprint BMP-7 as antagonist of organ fibrosis
    Ralf Weiskirchen
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Germany
    Front Biosci (Landmark Ed) 14:4992-5012. 2009
  4. pmc Elevated circulating soluble interleukin-2 receptor in patients with chronic liver diseases is associated with non-classical monocytes
    Sebastian Seidler
    Department of Medicine III, University Hospital, RWTH Aachen, Germany
    BMC Gastroenterol 12:38. 2012
  5. ncbi request reprint BMP-7 counteracting TGF-beta1 activities in organ fibrosis
    Ralf Weiskirchen
    RWTH University Hospital Aachen, Institute of Clinical Chemistry and Pathobiochemistry, Aachen, Germany
    Front Biosci (Landmark Ed) 18:1407-34. 2013
  6. pmc Expression analysis of inflammasomes in experimental models of inflammatory and fibrotic liver disease
    Sorina Georgiana Boaru
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Pauwelsstr, 30, Aachen D 52074, Germany
    J Inflamm (Lond) 9:49. 2012
  7. pmc Deficiency in four and one half LIM domain protein 2 (FHL2) aggravates liver fibrosis in mice
    Sebastian Huss
    Institute of Pathology, University of Cologne, Cologne, Germany
    BMC Gastroenterol 13:8. 2013
  8. pmc Development and evaluation of an open source Delphi-based software for morphometric quantification of liver fibrosis
    Sebastian Huss
    Institute of Pathology, University of Bonn, Bonn, Germany
    Fibrogenesis Tissue Repair 3:10. 2010
  9. pmc Interleukin-27 acts on hepatic stellate cells and induces signal transducer and activator of transcription 1-dependent responses
    Caroline Schoenherr
    Department of Biochemistry, University Hospital RWTH Aachen, Pauwelsstrasse 30, D 52074 Aachen, Germany
    Cell Commun Signal 8:19. 2010
  10. ncbi request reprint The murine latent transforming growth factor-beta binding protein (Ltbp-1) is alternatively spliced, and maps to a region syntenic to human chromosome 2p21-22
    Ralf Weiskirchen
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Pauwelsstrasse 30, Aachen 52074, Germany
    Gene 308:43-52. 2003

Detail Information

Publications99

  1. ncbi request reprint Analysis of the transforming growth factor-beta1 (TGF-beta1) codon 25 gene polymorphism by LightCycler-analysis in patients with chronic hepatitis C infection
    Carmen G Tag
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Pauwelsstr 30, D 52074, Aachen, Germany
    Cytokine 24:173-81. 2003
    ..This suggests that the stage of hepatic fibrosis, rather than the grade (inflammation), is influenced by the presence of proline at codon 25 in patients with chronic hepatitis C...
  2. ncbi request reprint TGF-beta up-regulates serum response factor in activated hepatic stellate cells
    Jens Herrmann
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, D 52074, Germany
    Biochim Biophys Acta 1772:1250-7. 2007
    ..Therefore, we conclude that hepatic stellate cells retain differentiation capacity to evolve characteristics that are typical for cells of the cardiac and smooth muscle lineages...
  3. ncbi request reprint BMP-7 as antagonist of organ fibrosis
    Ralf Weiskirchen
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Germany
    Front Biosci (Landmark Ed) 14:4992-5012. 2009
    ....
  4. pmc Elevated circulating soluble interleukin-2 receptor in patients with chronic liver diseases is associated with non-classical monocytes
    Sebastian Seidler
    Department of Medicine III, University Hospital, RWTH Aachen, Germany
    BMC Gastroenterol 12:38. 2012
    ....
  5. ncbi request reprint BMP-7 counteracting TGF-beta1 activities in organ fibrosis
    Ralf Weiskirchen
    RWTH University Hospital Aachen, Institute of Clinical Chemistry and Pathobiochemistry, Aachen, Germany
    Front Biosci (Landmark Ed) 18:1407-34. 2013
    ..We here summarize recent knowledge of BMP-7 function and discuss attempts to use this cytokine as a drug to reverse TGF-beta-induced fibrogenesis...
  6. pmc Expression analysis of inflammasomes in experimental models of inflammatory and fibrotic liver disease
    Sorina Georgiana Boaru
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Pauwelsstr, 30, Aachen D 52074, Germany
    J Inflamm (Lond) 9:49. 2012
    ....
  7. pmc Deficiency in four and one half LIM domain protein 2 (FHL2) aggravates liver fibrosis in mice
    Sebastian Huss
    Institute of Pathology, University of Cologne, Cologne, Germany
    BMC Gastroenterol 13:8. 2013
    ..Moreover, hepatic FHL2 is involved in regulation pathways mediating proliferation and cell death machineries. We here investigated the role of FHL2 in the setting of experimental and clinical liver fibrosis...
  8. pmc Development and evaluation of an open source Delphi-based software for morphometric quantification of liver fibrosis
    Sebastian Huss
    Institute of Pathology, University of Bonn, Bonn, Germany
    Fibrogenesis Tissue Repair 3:10. 2010
    ..Formation of fibrosis was determined with the developed software at day 0, 3, 7, 10, 14, 20, 30 and 60. The results were compared to a semi-quantitative scoring system...
  9. pmc Interleukin-27 acts on hepatic stellate cells and induces signal transducer and activator of transcription 1-dependent responses
    Caroline Schoenherr
    Department of Biochemistry, University Hospital RWTH Aachen, Pauwelsstrasse 30, D 52074 Aachen, Germany
    Cell Commun Signal 8:19. 2010
    ..The aim of this study was to investigate whether IL-27 would also act on hepatic stellate cells (HSC), the second most abundant hepatic cell type, which would demonstrate a more general role of this cytokine in the liver...
  10. ncbi request reprint The murine latent transforming growth factor-beta binding protein (Ltbp-1) is alternatively spliced, and maps to a region syntenic to human chromosome 2p21-22
    Ralf Weiskirchen
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Pauwelsstrasse 30, Aachen 52074, Germany
    Gene 308:43-52. 2003
    ..The utilization of different promoters and their evolutionarily conservation further emphasize the complex regulation of Ltbp-1...
  11. ncbi request reprint Pitfalls in LightCycler diagnosis of the single-nucleotide polymorphism 13.9 kb upstream of the lactase gene that is associated with adult-type hypolactasia
    Ralf Weiskirchen
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    Clin Chim Acta 384:93-8. 2007
    ..Recently, several novel allelic variants have been identified in non-European populations. Three of these variants occur in close proximity to C-13910T, but their effect on the genetic test is unknown...
  12. ncbi request reprint The CRP/MLP/TLP family of LIM domain proteins: acting by connecting
    Ralf Weiskirchen
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    Bioessays 25:152-62. 2003
    ....
  13. ncbi request reprint CCN proteins in normal and injured liver
    Ralf Weiskirchen
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, D 52074 Aachen, Pauwelsstr 30, Germany
    Front Biosci (Landmark Ed) 16:1939-61. 2011
    ..In this review, the actual knowledge of biological activities and molecular involvement of CCN proteins in maintenance of liver health and in initiation and progression of hepatic diseases is summarized and discussed...
  14. doi request reprint Protective effects of lipocalin-2 (LCN2) in acute liver injury suggest a novel function in liver homeostasis
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH Aachen University Hospital, Germany
    Biochim Biophys Acta 1832:660-73. 2013
    ..LCN2 levels provide no correlation to the degree of liver fibrosis but show significant positive correlation to inflammation instead...
  15. pmc Antifibrotic effects of CXCL9 and its receptor CXCR3 in livers of mice and humans
    Hermann E Wasmuth
    Department of Medicine III, University Hospital Aachen, Aachen, Germany
    Gastroenterology 137:309-19, 319.e1-3. 2009
    ..We investigated the roles of the chemokine CXCL9 and its receptor CXCR3 in liver fibrosis...
  16. ncbi request reprint Endoglin differentially modulates antagonistic transforming growth factor-beta1 and BMP-7 signaling
    Olaf Scherner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    J Biol Chem 282:13934-43. 2007
    ..Finally, we found that the transient overexpression of endoglin, previously shown to inhibit TGF-beta1-induced ALK-5/Smad3 signaling, enhanced the BMP-7/Smad1/Smad5 pathway...
  17. ncbi request reprint The expression of CSRP2 encoding the LIM domain protein CRP2 is mediated by TGF-beta in smooth muscle and hepatic stellate cells
    Jens Herrmann
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Germany
    Biochem Biophys Res Commun 345:1526-35. 2006
    ....
  18. ncbi request reprint Identification of endoglin in rat hepatic stellate cells: new insights into transforming growth factor beta receptor signaling
    Steffen K Meurer
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    J Biol Chem 280:3078-87. 2005
    ..In conclusion, we hypothesize that TGF-beta signals in HSC and MFB are tuned by two different interconnected signaling pathways, as it was previously demonstrated for endothelial cells...
  19. ncbi request reprint Dominant-negative soluble PDGF-beta receptor inhibits hepatic stellate cell activation and attenuates liver fibrosis
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    Lab Invest 84:766-77. 2004
    ....
  20. pmc Targeted disruption of the mouse Csrp2 gene encoding the cysteine- and glycine-rich LIM domain protein CRP2 result in subtle alteration of cardiac ultrastructure
    Julia F Sagave
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Germany
    BMC Dev Biol 8:80. 2008
    ..In order to analyze the function of CRP2 in vivo, we disrupted the Csrp2 gene in mice and analysed the resulting phenotype...
  21. doi request reprint Adenoviral CCN3/NOV gene transfer fails to mitigate liver fibrosis in an experimental bile duct ligation model because of hepatocyte apoptosis
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Aachen, Germany
    Liver Int 32:1342-53. 2012
    ..Recent studies have linked overexpression of CCN3/NOV to mitigate kidney fibrosis. This study aims to investigate CCN3/NOV overexpression in liver fibrogenesis in vivo...
  22. pmc Overexpression of endoglin modulates TGF-β1-signalling pathways in a novel immortalized mouse hepatic stellate cell line
    Steffen K Meurer
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Aachen, Germany
    PLoS ONE 8:e56116. 2013
    ..Furthermore it allows investigating the impact of various regulatory proteins (e.g. endoglin) on profibrogenic signal transduction, differentiation and hepatic stellate cell biology...
  23. doi request reprint Chemokine Cxcl9 attenuates liver fibrosis-associated angiogenesis in mice
    Hacer Sahin
    Medical Department III, University Hospital Aachen, Aachen, Germany
    Hepatology 55:1610-9. 2012
    ..Accordingly, systemic administration of Cxcl9 led to a strong attenuation of neoangiogenesis and experimental liver fibrosis in vivo...
  24. doi request reprint Activation of TGF-beta within cultured hepatocytes and in liver injury leads to intracrine signaling with expression of connective tissue growth factor
    Olav A Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    J Cell Mol Med 12:2717-30. 2008
    ....
  25. doi request reprint Induction of lipocalin-2 expression in acute and chronic experimental liver injury moderated by pro-inflammatory cytokines interleukin-1β through nuclear factor-κB activation
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH Aachen University Hospital, Aachen, Germany
    Liver Int 31:656-65. 2011
    ..Aims: In this study, we wanted to analyse the expression and regulation of LCN2 in models of acute and chronic experimental liver injury...
  26. ncbi request reprint Inhibition of hepatic fibrogenesis by matrix metalloproteinase-9 mutants in mice
    Martin Roderfeld
    Department of Internal Medicine III, RWTH University Hospital Aachen, Aachen, Germany
    FASEB J 20:444-54. 2006
    ..Application of MMP-9 mutants as TIMP-1 scavengers may provide a new therapeutic strategy for hepatic fibrosis...
  27. doi request reprint A functional variation in CHI3L1 is associated with severity of liver fibrosis and YKL-40 serum levels in chronic hepatitis C infection
    Marie Luise Berres
    Department of Internal Medicine III, University Hospital Aachen, RWTH Aachen, Pauwelsstr 30, D 52057 Aachen, Germany
    J Hepatol 50:370-6. 2009
    ..We assessed whether a functional promoter polymorphism in CHI3L1, encoding YKL-40, is associated with HCV-induced liver fibrosis and influences YKL-40 serum concentrations...
  28. doi request reprint A novel, dual role of CCN3 in experimental glomerulonephritis: pro-angiogenic and antimesangioproliferative effects
    Claudia R C van Roeyen
    Division of Nephrology and Clinical Immunology, RWTH Aachen University, Aachen, Germany
    Am J Pathol 180:1979-90. 2012
    ..Manipulation of CCN3 may represent a novel approach to help repair glomerular endothelial damage and mesangioproliferative changes...
  29. pmc Functional contribution of elevated circulating and hepatic non-classical CD14CD16 monocytes to inflammation and human liver fibrosis
    Henning W Zimmermann
    Department of Medicine III, University Hospital Aachen, Aachen, Germany
    PLoS ONE 5:e11049. 2010
    ....
  30. doi request reprint Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Aachen, Germany
    Lab Invest 88:1090-100. 2008
    ....
  31. ncbi request reprint Variable expression of cystatin C in cultured trans-differentiating rat hepatic stellate cells
    Axel M Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH Aachen University, D 52074 Aachen, Germany
    World J Gastroenterol 12:731-8. 2006
    ....
  32. doi request reprint Expression and functional analysis of endoglin in isolated liver cells and its involvement in fibrogenic Smad signalling
    Steffen K Meurer
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    Cell Signal 23:683-99. 2011
    ..In supernatants of respective cultures, we could detect the ectodomain of endoglin suggesting that shedding is a further key process involved in the regulation of this surface receptor...
  33. pmc Cyclin E1 controls proliferation of hepatic stellate cells and is essential for liver fibrogenesis in mice
    Yulia A Nevzorova
    Department of Medicine III, University Hospital, RWTH Aachen, Aachen, Germany
    Hepatology 56:1140-9. 2012
    ..In contrast, CcnE2-deficient HSCs expressed an elevated level of CcnE1 and showed enhanced cell-cycle activity and proliferation, compared to WT cells...
  34. doi request reprint Factor VII activating protease (FSAP) exerts anti-inflammatory and anti-fibrotic effects in liver fibrosis in mice and men
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    J Hepatol 58:104-11. 2013
    ..FSAP is activated by damaged cells and its substrates include growth factors and hemostasis proteins...
  35. doi request reprint Proteomic profiling in Lipocalin 2 deficient mice under normal and inflammatory conditions
    Kirsten Labbus
    Institute of Pathology, RWTH University Hospital Aachen, Germany
    J Proteomics 78:188-96. 2013
    ....
  36. doi request reprint Proapoptotic effects of the chemokine, CXCL 10 are mediated by the noncognate receptor TLR4 in hepatocytes
    Hacer Sahin
    Medical Department III and University Hospital Aachen, Aachen, Germany
    Hepatology 57:797-805. 2013
    ..Accordingly, systemic application of CXCL10 led to TLR4-induced liver cell apoptosis in vivo...
  37. ncbi request reprint The fractalkine receptor CX3CR1 is involved in liver fibrosis due to chronic hepatitis C infection
    Hermann E Wasmuth
    Medical Department III, University Hospital Aachen, Pauwelsstrasse 30, D 52057 Aachen, Germany
    J Hepatol 48:208-15. 2008
    ..The chemokine receptor CX3CR1 and its specific ligand fractalkine (CX3CL1) are known to modulate inflammatory and fibroproliferative diseases. Here we investigate the role of CX3CR1/fractalkine in HCV-induced liver fibrosis...
  38. doi request reprint Intracrine signalling of activin A in hepatocytes upregulates connective tissue growth factor (CTGF/CCN2) expression
    Olav A Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    Liver Int 28:1207-16. 2008
    ....
  39. pmc Antagonism of the chemokine Ccl5 ameliorates experimental liver fibrosis in mice
    Marie Luise Berres
    Medical Department III, University Hospital Aachen, Aachen, Germany
    J Clin Invest 120:4129-40. 2010
    ..Our results define a successful therapeutic approach to reduce experimental liver fibrosis by antagonizing Ccl5 receptors...
  40. doi request reprint CXC chemokine ligand 4 (Cxcl4) is a platelet-derived mediator of experimental liver fibrosis
    Mirko Moreno Zaldivar
    Department of Medicine III, University Hospital Aachen, Aachen, Germany
    Hepatology 51:1345-53. 2010
    ..Conclusion: The results underscore an important role of platelets in chronic liver damage and imply a new target for antifibrotic therapies...
  41. pmc Human intestinal acyl-CoA synthetase 5 is sensitive to the inhibitor triacsin C
    Elke Kaemmerer
    Department of Pediatrics, RWTH Aachen University, Aachen, Germany
    World J Gastroenterol 17:4883-9. 2011
    ..To investigate whether human acyl-CoA synthetase 5 (ACSL5) is sensitive to the ACSL inhibitor triacsin C...
  42. pmc Analysis of polymorphic TGFB1 codons 10, 25, and 263 in a German patient group with non-syndromic cleft lip, alveolus, and palate compared with healthy adults
    Christian Stoll
    Department of Oral and Maxillofacial Surgery, University of Aachen, Pauwelsstrasse 30, D 52074 Aachen, Germany
    BMC Med Genet 5:15. 2004
    ..Leu10Pro and Arg25Pro polymorphisms in the precursor region and Thr263Ile polymorphism in the prodomain of the transforming growth factor beta1 (TGF-beta1) gene have proved to be crucial to predisposition of several disorders...
  43. ncbi request reprint Cryopreservation of hepatic stellate cells
    Svenja Neyzen
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Pauwelsstr 30, D 52074 Aachen, Germany
    J Hepatol 44:910-7. 2006
    ..Thus, there is a continuing need for developing methods allowing the exchange of HSC isolates between different laboratories. A practical solution to this problem is cryopreservation and banking of HSC...
  44. ncbi request reprint CSRP2, TIMP-1, and SM22alpha promoter fragments direct hepatic stellate cell-specific transgene expression in vitro, but not in vivo
    Jens Herrmann
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    Liver Int 24:69-79. 2004
    ..The corresponding regulatory elements of these genes have afforded us the opportunity to express transgenes with antifibrotic potential in a cell type- and/or transdifferentiation-dependent manner...
  45. doi request reprint Elevated asymmetric dimethylarginine levels predict short- and long-term mortality risk in critically ill patients
    Alexander Koch
    Department of Medicine III, RWTH University Hospital Aachen, Pauwelsstrasse 30, Aachen, Germany
    J Crit Care 28:947-53. 2013
    ..We aimed at investigating ADMA levels as a potential diagnostic or prognostic biomarker in critically ill patients...
  46. doi request reprint From proteomic multimarker profiling to interesting proteins: thymosin-β(4) and kininogen-1 as new potential biomarkers for inflammatory hepatic lesions
    Corinna Henkel
    Institute of Pathology, RWTH University Hospital Aachen, Aachen, Germany
    J Cell Mol Med 15:2176-88. 2011
    ..Based on the high throughput capability, the identified differential protein panel may serve as a diagnostic marker and warrants further validation in larger cohorts...
  47. doi request reprint Lipid-induced up-regulation of human acyl-CoA synthetase 5 promotes hepatocellular apoptosis
    Andrea Reinartz
    Institute of Pathology, RWTH Aachen University, Aachen, Germany
    Biochim Biophys Acta 1801:1025-35. 2010
    ..We propose that ACSL5 could play a role in promoting fatty acid-induced lipoapoptosis in hepatocytes as important mechanism in fatty liver-related disorders...
  48. doi request reprint The Marburg I variant (G534E) of the factor VII-activating protease determines liver fibrosis in hepatitis C infection by reduced proteolysis of platelet-derived growth factor BB
    Hermann E Wasmuth
    Department of Internal Medicine III, University Hospital Aachen, Aachen, Germany
    Hepatology 49:775-80. 2009
    ..FSAP G534E might be useful for risk stratification in patients with HCV infection...
  49. doi request reprint Pharmacological application of caffeine inhibits TGF-beta-stimulated connective tissue growth factor expression in hepatocytes via PPARgamma and SMAD2/3-dependent pathways
    Olav A Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, 52074 Aachen, Germany
    J Hepatol 49:758-67. 2008
    ..Epidemiological studies suggest that coffee drinking is inversely correlated with the risk of development of liver fibrosis but the molecular basis is unknown...
  50. doi request reprint Evaluation of a novel reverse-hybridization StripAssay for typing DNA variants useful in diagnosis of adult-type hypolactasia
    Carmen G Tag
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    Clin Chim Acta 392:58-62. 2008
    ..We have recently shown that several novel allelic variants located in close proximity to the C-13910T SNP interfere with the diagnostic accuracy of real-time PCR-based genotyping methods...
  51. doi request reprint Platelet-derived growth factor (PDGF)-C neutralization reveals differential roles of PDGF receptors in liver and kidney fibrosis
    Ina V Martin
    Division of Nephrology and Clinical Immunology, RWTH Aachen University, Aachen, Germany
    Am J Pathol 182:107-17. 2013
    ..PDGF-C antagonism, therefore, may not be effective to treat liver fibrosis...
  52. pmc Aberrant cell cycle progression and endoreplication in regenerating livers of mice that lack a single E-type cyclin
    Yulia A Nevzorova
    Department of Medicine III, University Hospital Aachen, RWTH Aachen University, Aachen, Germany
    Gastroenterology 137:691-703, 703.e1-6. 2009
    ..We investigated the relevance of E-cyclins in directing resting hepatocytes into the cell cycle after partial hepatectomy using CcnE1 and CcnE2 knockout mice...
  53. ncbi request reprint Differential effects of TGF-beta on connective tissue growth factor (CTGF/CCN2) expression in hepatic stellate cells and hepatocytes
    Olav A Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    J Hepatol 47:699-710. 2007
    ..Connective tissue growth factor (CTGF/CCN2) has been implicated in the pathogenesis of hepatic fibrosis and suggested as a downstream mediator of the fibrogenic master cytokine TGF-beta...
  54. ncbi request reprint N-acetyl-L-cysteine suppresses TGF-beta signaling at distinct molecular steps: the biochemical and biological efficacy of a multifunctional, antifibrotic drug
    Steffen K Meurer
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Pauwelsstr 30, D 52074 Aachen, Germany
    Biochem Pharmacol 70:1026-34. 2005
    ..In conclusion, these findings suggest that this non-toxic aminothiol downregulates TGF-beta signal transduction thereby mediating beneficial effects on experimental liver fibrosis characterized by TGF-beta hyperactivity...
  55. ncbi request reprint Disruption of the latent transforming growth factor-beta binding protein-1 gene causes alteration in facial structure and influences TGF-beta bioavailability
    Falko Drews
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Pauwelsstr 30, D 52074 Aachen, Germany
    Biochim Biophys Acta 1783:34-48. 2008
    ..Therefore, we conclude that LTBP-1 has essential functions in the control of TGF-beta activation...
  56. doi request reprint Analysis of antigen-presenting functionality of cultured rat hepatic stellate cells and transdifferentiated myofibroblasts
    Michael Bomble
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    Biochem Biophys Res Commun 396:342-7. 2010
    ..Based on our findings we conclude that HSC play a key role in hepatic immune function and that IFN-gamma treatment might mediate its beneficial therapeutic effects via activation of APC function in MFB...
  57. ncbi request reprint Complement factor 5 is a quantitative trait gene that modifies liver fibrogenesis in mice and humans
    Sonja Hillebrandt
    Department of Medicine III, University Hospital Aachen, Aachen University, Aachen, Germany
    Nat Genet 37:835-43. 2005
    ..Thus, the mouse quantitative trait gene led to the identification of an unknown gene underlying human susceptibility to liver fibrosis, supporting the idea that C5 has a causal role in fibrogenesis across species...
  58. doi request reprint BMP-7/TGF-β1 signalling in myoblasts: components involved in signalling and BMP-7-dependent blockage of TGF-β-mediated CTGF expression
    Steffen K Meurer
    The Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Pauwelsstr 30, D 52074 Aachen, Germany
    Eur J Cell Biol 91:450-63. 2012
    ..These findings point towards a regulatory role of BMP-7 that relies on modulation of Mitogen-activated protein kinases rather than mechanisms that are exclusively driven by differential Smad activation...
  59. pmc Recombinant expression, purification, and functional characterisation of connective tissue growth factor and nephroblastoma-overexpressed protein
    Wilhelm Bohr
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Aachen, Germany
    PLoS ONE 5:e16000. 2010
    ..We further demonstrate for the first time that both recombinant CCN proteins are N-glycosylated...
  60. ncbi request reprint Adenoviral delivery of an antisense RNA complementary to the 3' coding sequence of transforming growth factor-beta1 inhibits fibrogenic activities of hepatic stellate cells
    Monica Arias
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    Cell Growth Differ 13:265-73. 2002
    ....
  61. ncbi request reprint Disruption of intermolecular disulfide bonds in PDGF-BB dimers by N-acetyl-L-cysteine does not prevent PDGF signaling in cultured hepatic stellate cells
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Germany
    Biochem Biophys Res Commun 338:1711-8. 2005
    ..Therefore, we conclude that the PDGF monomer is still active. The described antifibrotic effects are therefore mainly attributable to the structural impairment of TGF-beta signaling components reported previously...
  62. ncbi request reprint Induction of cell death in activated hepatic stellate cells by targeted gene expression of the thymidine kinase/ganciclovir system
    Nora Janoschek
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    Biochem Biophys Res Commun 316:1107-15. 2004
    ..Altogether, these results indicate that induction of programmed cell death is a promising approach to eliminate fibrogenic HSC...
  63. pmc Adenoviral expression of a transforming growth factor-beta1 antisense mRNA is effective in preventing liver fibrosis in bile-duct ligated rats
    Monica Arias
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, D 52074, Germany
    BMC Gastroenterol 3:29. 2003
    ....
  64. pmc Circulating retinol binding protein 4 in critically ill patients before specific treatment: prognostic impact and correlation with organ function, metabolism and inflammation
    Alexander Koch
    Department of Medicine III, RWTH University Hospital Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany
    Crit Care 14:R179. 2010
    ..Retinol binding protein 4 (RBP4) promotes insulin resistance in mice and is systemically elevated in patients with obesity and type 2 diabetes. We investigated the potential role of RBP4 in critically ill patients...
  65. ncbi request reprint Isolation and culture of hepatic stellate cells
    Ralf Weiskirchen
    Institute of Clinical Chemistry and Pathobiochemistry, University Hospital Aachen, Aachen, Germany
    Methods Mol Med 117:99-113. 2005
    ..Further purification by centrifugal elutriation results in virtually pure HSC preparations ( >98%)...
  66. ncbi request reprint Pro-fibrogenic potential of PDGF-D in liver fibrosis
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, and Division of Nephrology, RWTH University Hospital Aachen, D 52074 Aachen, Germany
    J Hepatol 46:1064-74. 2007
    ..We analyzed the expression of platelet-derived growth factor D (PDGF-D) in an experimental bile duct-ligated (BDL) rat model and assessed its biological function in cultured hepatic stellate cells (HSC) and myofibroblasts (MFB)...
  67. ncbi request reprint Biomarkers of liver fibrosis: clinical translation of molecular pathogenesis or based on liver-dependent malfunction tests
    Olav A Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Central Laboratory, Pauwelsstr 30, 52074 Aachen, Germany
    Clin Chim Acta 381:107-13. 2007
    ....
  68. ncbi request reprint Insulin resistance in liver cirrhosis is not associated with circulating retinol-binding protein 4
    Eray Yagmur
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, 52074 Aachen, Germany
    Diabetes Care 30:1168-72. 2007
    ..As insulin resistance is present in nearly all patients with liver cirrhosis, we evaluated RBP4 in patients with chronic liver disease (CLD)...
  69. ncbi request reprint Antisense strategy against PDGF B-chain proves effective in preventing experimental liver fibrogenesis
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Germany
    Biochem Biophys Res Commun 321:413-23. 2004
    ..The asPDGF mRNA also attenuates experimental liver fibrogenesis indicated by reduced levels of alpha-SMA and collagen type I expression...
  70. doi request reprint Proteomic tissue profiling for the improvement of grading of noninvasive papillary urothelial neoplasia
    Rena F Oezdemir
    Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany
    Clin Biochem 45:7-11. 2012
    ..Matrix Assisted Laser Desorption/Ionization Time of Flight Imaging Mass Spectrometry (MALDI TOF IMS) was evaluated as an automatic and objective tool to assist grading of urothelial neoplasms and to facilitate accuracy...
  71. ncbi request reprint Inhibitory effect of soluble PDGF-beta receptor in culture-activated hepatic stellate cells
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen D 52074, Germany
    Biochem Biophys Res Commun 317:451-62. 2004
    ..In summary, sPDGFRbeta clearly shows effective inhibitory properties in early HSC activation, suggesting potential therapeutic impact for anti-PDGF intervention in liver fibrogenesis...
  72. doi request reprint Connective tissue growth factor is a Smad2 regulated amplifier of transforming growth factor beta actions in hepatocytes--but without modulating bone morphogenetic protein 7 signaling
    Olav A Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    Hepatology 49:2021-30. 2009
    ....
  73. doi request reprint Changing the pathogenetic roadmap of liver fibrosis? Where did it start; where will it go?
    Olav A Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany
    J Gastroenterol Hepatol 23:1024-35. 2008
    ..g. liver, lung, kidney), and offer innovative approaches for the development of non-invasive biomarkers and antifibrotic trials...
  74. doi request reprint Hepatic recruitment of the inflammatory Gr1+ monocyte subset upon liver injury promotes hepatic fibrosis
    Karlin Raja Karlmark
    Department of Medicine III, RWTH University Hospital Aachen, Aachen, Germany
    Hepatology 50:261-74. 2009
    ..Thus, they may represent an interesting novel target for antifibrotic strategies...
  75. ncbi request reprint Rapid detection of CSRP2 mRNA in mouse, rat, and human using LightCycler-based quantitative real-time polymerase chain reaction
    Kalle Günther
    Urological Clinic, RWTH University Hospital, Pauwelsstr 30, D 52074 Aachen, Germany
    Anal Biochem 314:144-8. 2003
  76. ncbi request reprint Adenoviral-mediated transfer of p53 or retinoblastoma protein blocks cell proliferation and induces apoptosis in culture-activated hepatic stellate cells
    Bärbel Abriss
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074, Aachen, Germany
    J Hepatol 38:169-78. 2003
    ..Thus, removal of activated HSC should be beneficial for the prognosis of hepatic fibrogenesis and preserve organ function...
  77. ncbi request reprint Immortal hepatic stellate cell lines: useful tools to study hepatic stellate cell biology and function?
    Jens Herrmann
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    J Cell Mol Med 11:704-22. 2007
    ..Here, we summarize the history and cellular characteristics of respective cell lines and discuss the differences of continuous HSC lines and their primary counterparts...
  78. doi request reprint Serum proteomic profiling in patients with bladder cancer
    Kristina Schwamborn
    Institute of Pathology, Department of Urology, RWTH Aachen University, Aachen, Germany
    Eur Urol 56:989-96. 2009
    ..Despite continuing research for accurate bladder cancer biomarkers, the analytes suffer from lack of sensitivity and specificity...
  79. doi request reprint Maternal factor V Leiden mutation is associated with HELLP syndrome in Caucasian women
    Sabine Muetze
    Department of Obstetrics and Gynecology, RWTH Aachen University Hospital, Aachen, Germany
    Acta Obstet Gynecol Scand 87:635-42. 2008
    ....
  80. pmc CCN3/NOV small interfering RNA enhances fibrogenic gene expression in primary hepatic stellate cells and cirrhotic fat storing cell line CFSC
    Erawan Borkham-Kamphorst
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, 52074, Aachen, Germany
    J Cell Commun Signal 6:11-25. 2012
    ..These results indicate the complexity of CCN actions that are far beyond the classic Yin/Yang interplay...
  81. ncbi request reprint Roles of TGF-beta in hepatic fibrosis
    Axel M Gressner
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    Front Biosci 7:d793-807. 2002
    ....
  82. doi request reprint Non-synonymous gene polymorphisms in the secretory signal peptide of human TGF-beta1 affect cellular synthesis but not secretion of TGF-beta1
    Simone Mohren
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, D 52074 Aachen, Germany
    Biochem Biophys Res Commun 379:1015-20. 2009
    ..Detailed RNA structure prediction analysis further suggests that the individual variants form different secondary structures...
  83. doi request reprint TAK1 suppresses a NEMO-dependent but NF-kappaB-independent pathway to liver cancer
    Kira Bettermann
    Department of Internal Medicine III, University Hospital RWTH Aachen, D 52074 Aachen, Germany
    Cancer Cell 17:481-96. 2010
    ..Therefore, TAK1 serves as a gatekeeper for a protumorigenic, NF-kappaB-independent function of NEMO in parenchymal liver cells...
  84. pmc Fatty acid binding receptors in intestinal physiology and pathophysiology
    Elke Kaemmerer
    Elke Kaemmerer, Angela Schippers, Norbert Wagner, Department of Pediatrics, RWTH Aachen University, Aachen 52074, Germany
    World J Gastrointest Pathophysiol 1:147-53. 2010
    ..In this review, an overview of intestinal fatty acid binding receptors and their role in intestinal physiology and pathophysiology is given...
  85. ncbi request reprint Occult celiac disease prevents penetrance of hemochromatosis
    Andreas Geier
    Department of Internal Medicine III, Aachen University, Pauwelsstrasse 30, D 52074 Aachen, Germany
    World J Gastroenterol 11:3323-6. 2005
    ..To report a patient with C282Y homozygocity, depleted body iron and intestinal atrophy caused by celiac disease (CD) who experienced resolution of the enteropathy with subsequent normalization of iron metabolism upon gluten-free diet...
  86. pmc Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders
    Pornwilard M-M
    Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital Aachen, Aachen, Germany
    PLoS ONE 8:e58702. 2013
    ..Established in situ techniques, such as EDX-ray spectroscopy, are not sensitive enough to analyze trace metal distribution and the quantification of metal images is difficult...
  87. ncbi request reprint Common heterozygous hemochromatosis gene mutations are risk factors for inflammation and fibrosis in chronic hepatitis C
    Andreas Geier
    Department of Medicine III, University Hospital Aachen, Aachen University RWTH, Germany
    Liver Int 24:285-94. 2004
    ..Therefore our aim was to assess associations between HFE mutations and hepatic inflammation and stage of fibrosis in German hepatitis C patients...
  88. ncbi request reprint Transforming growth factor-beta1 gene polymorphisms are associated with progression of liver fibrosis in Caucasians with chronic hepatitis C infection
    Hao Wang
    Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China
    World J Gastroenterol 11:1929-36. 2005
    ..For this reason, much emphasis has been placed on studies elucidating the impact of TGF-beta1 and its gene variations for the susceptibility and pathogenesis of these diseases. Unfortunately, some studies have serious limitations...
  89. ncbi request reprint Characterization of Sno expression in malignant melanoma
    Ina Poser
    University of Regensburg Medical School, D 93053 Regensburg, Germany
    Int J Oncol 26:1411-7. 2005
    ..In summary, constitutive Sno expression was identified as an important mechanism to shut off antiproliferative TGF-beta signaling in malignant melanoma...
  90. ncbi request reprint Atypical melting curve resulting from genetic variation in the 3' untranslated region at position 20218 in the prothrombin gene analyzed with the LightCycler factor II (prothrombin) G20210A assay
    Carmen G Tag
    Clin Chem 51:1560-1. 2005
  91. ncbi request reprint Transforming growth factor-beta gene polymorphisms in sarcoidosis patients with and without fibrosis
    Adrian Kruit
    Department of Pulmonology, St Antonius Hospital, Koekoekslaan 1, 3435 Nieuwegein, The Netherlands
    Chest 129:1584-91. 2006
    ..We determined whether single-nucleotide polymorphisms (SNPs) in the TGF-beta1, TGF-beta2, and TGF-beta3 genes might contribute to pulmonary fibrosis in sarcoidosis patients...
  92. ncbi request reprint A novel proximal -13914G>A base replacement in the vicinity of the common-13910T/C lactase gene variation results in an atypical LightCycler melting curve in testing with the MutaREAL Lactase test
    Carmen G Tag
    Clin Chem 53:146-8. 2007
  93. doi request reprint Transforming growth factor beta 1 gene variants increase transcription and are associated with liver cirrhosis in Chinese
    Hao Wang
    Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, China
    Cytokine 43:20-5. 2008
    ..Transforming growth factor beta1 (TGFbeta1) acts as an important profibrogenic cytokine in liver fibrogenesis. The aim of this study was to explore the association between TGFbeta1 gene polymorphisms and liver cirrhosis...
  94. ncbi request reprint Rapid genotyping of the G534E polymorphism (Marburg I) of the gene encoding the factor VII-activating protease (FSAP) by LightCycler PCR
    Carmen G Tag
    Clin Biochem 40:1063-4. 2007
  95. ncbi request reprint The tightrope of therapeutic suppression of active transforming growth factor-beta: high enough to fall deeply?
    Axel M Gressner
    J Hepatol 39:856-9. 2003
  96. ncbi request reprint Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases
    Obul Reddy Bandapalli
    Institute of Biology, Humboldt University Berlin, Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Int J Cancer 118:74-89. 2006
    ..In summary, our data demonstrate the feasibility of an interspecies differential gene expression approach on a genomewide scale...
  97. ncbi request reprint Changes of the hepatic proteome in murine models for toxically induced fibrogenesis and sclerosing cholangitis
    Corinna Henkel
    University Hospital Giessen and Marburg, Campus Giessen, Department of Medicine II, Gastroenterology, Giessen, Germany
    Proteomics 6:6538-48. 2006
    ..The only novel protein with regard to liver fibrosis depicting a unidirectional expression pattern in both animal models was Sbp2. An explicit protein function could not be clarified yet...
  98. doi request reprint Liver function critically determines serum retinol-binding protein 4 (RBP4) levels in patients with chronic liver disease and cirrhosis
    Frank Tacke
    Hepatology 48:1724-5; author reply 1725-6. 2008
  99. ncbi request reprint Ito cells are liver-resident antigen-presenting cells for activating T cell responses
    Florian Winau
    Department of Immunology, Max Planck Institute for Infection Biology, Schumannstrasse 21 22, 10117 Berlin, Germany
    Immunity 26:117-29. 2007
    ....