Affiliation: University of Freiburg
Wagner H, Wehrle S, Weiss E, Cavallari M, Weber W. A Two-Step Approach for the Design and Generation of Nanobodies. Int J Mol Sci. 2018;19: pubmed publisher
..We suggest that this strategy can complement existing immune, naïve, and synthetic library based methods, requiring neither animal experiments, nor large libraries, nor sophisticated selection protocols. ..
Baaske J, Gonschorek P, Engesser R, Dominguez Monedero A, Raute K, Fischbach P, et al
. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep. 2018;8:15024 pubmed publisher
..As exemplified here for the control of apoptosis in mammalian cell culture, the approach opens up novel perspectives in fundamental research and applications such as tissue engineering. ..
Beyer H, Thomas O, Riegel N, Zurbriggen M, Weber W, Hörner M. Generic and reversible opto-trapping of biomolecules. Acta Biomater. 2018;79:276-282 pubmed publisher
..This platform allows the use of red and far-red light to spatiotemporally control binding and release of arbitrary PIF-fused biomolecules from various material scaffolds. ..
MÃ¼ller K, Zurbriggen M, Weber W. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng. 2015;112:1483-7 pubmed publisher
..This approach resulted in an optogenetic upgrade of the popular Tet-OFF (TetR-VP64, tetO7 -PhCMVmin ) system that allows tunable, blue light-inducible transgene expression in HEK-293T cells. ..
Wagner H, Sprenger A, Rebmann B, Weber W. Upgrading biomaterials with synthetic biological modules for advanced medical applications. Adv Drug Deliv Rev. 2016;105:77-95 pubmed publisher
Fischer A, Weber W, Warscheid B, Radziwill G. AKT-dependent phosphorylation of the SAM domain induces oligomerization and activation of the scaffold protein CNK1. Biochim Biophys Acta Mol Cell Res. 2017;1864:89-100 pubmed publisher
..Our findings reveal SAM domain-dependent oligomerization by AKT as switch for CNK1 activation. ..
Kolar K, Weber W. Synthetic biological approaches to optogenetically control cell signaling. Curr Opin Biotechnol. 2017;47:112-119 pubmed publisher
..Besides, they hold a potential for further development into biomedical applications, from in vitro optogenetics-assisted drug candidate screenings to light-controlled gene therapy and tissue engineering. ..
Hörner M, Muller K, Weber W. Light-Responsive Promoters. Methods Mol Biol. 2017;1651:173-186 pubmed publisher
..Afterward, we give a detailed protocol for the implementation of a UVB light-inducible expression system in mammalian cells. ..
Hörner M, Chatelle C, Mühlhäuser W, Stocker D, Coats M, Weber W, et al
. Optogenetic control of focal adhesion kinase signaling. Cell Signal. 2018;42:176-183 pubmed publisher