Affiliation: University of Regensburg
- Development and immunological assessment of VLP-based immunogens exposing the membrane-proximal region of the HIV-1 gp41 proteinThomas D Benen
Molecular Microbiology and Gene Therapy Unit, Institute of Medical Microbiology and Hygiene, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, 93053, Germany
J Biomed Sci 21:79. 2014..Epitope focusing and stabilization present promising strategies to enhance the quality of immune responses to specific epitopes...
- CpG domains downstream of TSSs promote high levels of gene expressionSimone Krinner
Department of Molecular Microbiology and Gene Therapy, Institute of Medical Microbiology and Hygiene, University Hospital of Regensburg, Franz Josef Straus Allee 11, 93053 Regensburg, Germany and Department of Biochemistry III, Institute for Biochemistry, Genetics and Microbiology, University of Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
Nucleic Acids Res 42:3551-64. 2014..We suggest that these 5' CpG domains are required to distort the chromatin structure and to increase gene activity. ..
- Comprehensive analysis of interactions between the Src-associated protein in mitosis of 68 kDa and the human Src-homology 3 proteomeBenedikt Asbach
Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany
PLoS ONE 7:e38540. 2012..Thus, we propose, that Sam68 functions as a classical scaffold protein in this context, assembling components of an osteoclast-specific signalling pathway...
- Altering an artificial Gagpolnef polyprotein and mode of ENV co-administration affects the immunogenicity of a clade C HIV DNA vaccineKatharina Böckl
Institute of Medical Microbiology, University of Regensburg, Regensburg, Germany
PLoS ONE 7:e34723. 2012..These results are guiding the design of ongoing pre-clinical and clinical trials...
- The impact of intragenic CpG content on gene expressionAsli Petra Bauer
Institute of Medical Microbiology and Hygiene, Molecular Microbiology and Gene Therapy Unit, University of Regensburg, Regensburg, Germany
Nucleic Acids Res 38:3891-908. 2010..Increased high CpG transcription correlated with changed nucleosomal positions in vitro albeit histone density at the two genes did not change in vivo as monitored by ChIP...
- Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectorsSandra Siegert
Georg Speyer Haus, Institute for Biomedical Research, Paul Ehrlich Strasse 42 44, D 60596 Frankfurt am Main, Germany
AIDS Res Ther 2:7. 2005..Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry...
- Correlates of protection, antigen delivery and molecular epidemiology: basics for designing an HIV vaccineR Wagner
Institute of Medical Microbiology and Hygiene University of Regensburg, Germany
Vaccine 17:1706-10. 1999..A molecular clone of a representative virus strain is now available for the development of innovative antigen delivery systems aiming to be evaluated in future clinical vaccine trials throughout this area...
- Construction, expression, and immunogenicity of chimeric HIV-1 virus-like particlesR Wagner
Institute of Medical Microbiology, University of Regensburg, Germany
Virology 220:128-40. 1996..These results indicate ways for the rational design and optimal delivery of CTL-stimulating HIV candidate vaccines...
- Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene designD Kosovac
Molecular Microbiology and Gene Therapy Unit, Institute of Medical Microbiology, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, Germany
Gene Ther 18:189-98. 2011..5 μg were sufficient to trigger optimal long-term hematologic effects encouraging the use of sequence-optimized transgenes to further reduce effective pDNA amounts...
- Multiple effects of codon usage optimization on expression and immunogenicity of DNA candidate vaccines encoding the human immunodeficiency virus type 1 Gag proteinL Deml
Institute of Medical Microbiology, University of Regensburg, 93053 Regensburg, Germany
J Virol 75:10991-1001. 2001..These results suggest that increased Gag expression, rather than modulation of CpG-driven vector immunity, is responsible for the enhanced immunogenicity of the syngag DNA vaccine...
- Proline residues in the HIV-1 NH2-terminal capsid domain: structure determinants for proper core assembly and subsequent steps of early replicationT Fitzon
Institute of Medical Microbiology, Institute of Pathology, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, D 93053, Germany
Virology 268:294-307. 2000..These results suggest that proline residues in the NH(2)-terminal capsid domain represent critical structure determinants for proper formation of functional virion cores and subsequent stages of early replication...
- Inhibition of wild-type human immunodeficiency virus and reverse transcriptase inhibitor-resistant variants by Phyllanthus amarusF Notka
CMI Centers for Medical Innovation AG, Fraunhofer Str 15, 82152 Martinsried, Germany
Antiviral Res 58:175-86. 2003..8 to 14.6 microg/ml. The ability to inhibit replication of a variety of RT inhibitor-resistant HIV-1 strains points to the potential of P. amarus extracts, as natural products, in the chemotherapy of HIV infections...
- A new approach to an influenza live vaccine: modification of the cleavage site of hemagglutininJ Stech
Institut fuer Virologie, Klinikum der Philipps Universitaet Marburg, Postfach 2360, 35011 Marburg, Germany
Nat Med 11:683-9. 2005..At a dose of 10(5) plaque-forming units, it induced complete protection against lethal challenge. This approach allows the conversion of any epidemic strain into a genetically homologous attenuated virus...
- [Status and current strategies of HIV vaccine development]J Wild
Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Regensburg
Internist (Berl) 44:711-8. 2003..The problems in the development of an HIV-vaccine as well as former and current strategies to overcome these problems are presented here. The current status of human studies of different candidate vaccines is outlined...