R Wagner

Summary

Affiliation: University of Regensburg
Country: Germany

Publications

  1. pmc CpG domains downstream of TSSs promote high levels of gene expression
    Simone Krinner
    Department of Molecular Microbiology and Gene Therapy, Institute of Medical Microbiology and Hygiene, University Hospital of Regensburg, Franz Josef Straus Allee 11, 93053 Regensburg, Germany and Department of Biochemistry III, Institute for Biochemistry, Genetics and Microbiology, University of Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Nucleic Acids Res 42:3551-64. 2014
  2. pmc Comprehensive analysis of interactions between the Src-associated protein in mitosis of 68 kDa and the human Src-homology 3 proteome
    Benedikt Asbach
    Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany
    PLoS ONE 7:e38540. 2012
  3. pmc Altering an artificial Gagpolnef polyprotein and mode of ENV co-administration affects the immunogenicity of a clade C HIV DNA vaccine
    Katharina Böckl
    Institute of Medical Microbiology, University of Regensburg, Regensburg, Germany
    PLoS ONE 7:e34723. 2012
  4. pmc The impact of intragenic CpG content on gene expression
    Asli Petra Bauer
    Institute of Medical Microbiology and Hygiene, Molecular Microbiology and Gene Therapy Unit, University of Regensburg, Regensburg, Germany
    Nucleic Acids Res 38:3891-908. 2010
  5. pmc Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
    Sandra Siegert
    Georg Speyer Haus, Institute for Biomedical Research, Paul Ehrlich Strasse 42 44, D 60596 Frankfurt am Main, Germany
    AIDS Res Ther 2:7. 2005
  6. ncbi request reprint Correlates of protection, antigen delivery and molecular epidemiology: basics for designing an HIV vaccine
    R Wagner
    Institute of Medical Microbiology and Hygiene University of Regensburg, Germany
    Vaccine 17:1706-10. 1999
  7. ncbi request reprint Construction, expression, and immunogenicity of chimeric HIV-1 virus-like particles
    R Wagner
    Institute of Medical Microbiology, University of Regensburg, Germany
    Virology 220:128-40. 1996
  8. doi request reprint Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design
    D Kosovac
    Molecular Microbiology and Gene Therapy Unit, Institute of Medical Microbiology, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, Germany
    Gene Ther 18:189-98. 2011
  9. pmc Multiple effects of codon usage optimization on expression and immunogenicity of DNA candidate vaccines encoding the human immunodeficiency virus type 1 Gag protein
    L Deml
    Institute of Medical Microbiology, University of Regensburg, 93053 Regensburg, Germany
    J Virol 75:10991-1001. 2001
  10. ncbi request reprint Proline residues in the HIV-1 NH2-terminal capsid domain: structure determinants for proper core assembly and subsequent steps of early replication
    T Fitzon
    Institute of Medical Microbiology, Institute of Pathology, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, D 93053, Germany
    Virology 268:294-307. 2000

Collaborators

  • R Schirmbeck
  • Kalle Saksela
  • M Niedrig
  • F Notka
  • Simone Krinner
  • J Wild
  • Gernot Längst
  • Benedikt Asbach
  • Katharina Böckl
  • D Kosovac
  • Christine Ludwig
  • Asli Petra Bauer
  • Sandra Siegert
  • J Stech
  • Asli P Heitzer
  • Ingrid Obermeier
  • Sarah D Diermeier
  • Jens Wild
  • Josef Kostler
  • L Deml
  • Simon Bredl
  • Kathrin Kindsmüller
  • S Meissner
  • C Ludwig
  • A P Bauer
  • H Wolf
  • Doris Leikam
  • T Fitzon
  • Barbara S Schnierle
  • Sonja Thaler
  • M Wegmann
  • H D Klenk
  • H Garn
  • S Steck
  • A Bojak
  • M Graf
  • C Paulus
  • K Bieler
  • B Leschonsky
  • J Schroder

Detail Information

Publications13

  1. pmc CpG domains downstream of TSSs promote high levels of gene expression
    Simone Krinner
    Department of Molecular Microbiology and Gene Therapy, Institute of Medical Microbiology and Hygiene, University Hospital of Regensburg, Franz Josef Straus Allee 11, 93053 Regensburg, Germany and Department of Biochemistry III, Institute for Biochemistry, Genetics and Microbiology, University of Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Nucleic Acids Res 42:3551-64. 2014
    ..We suggest that these 5' CpG domains are required to distort the chromatin structure and to increase gene activity. ..
  2. pmc Comprehensive analysis of interactions between the Src-associated protein in mitosis of 68 kDa and the human Src-homology 3 proteome
    Benedikt Asbach
    Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany
    PLoS ONE 7:e38540. 2012
    ..Thus, we propose, that Sam68 functions as a classical scaffold protein in this context, assembling components of an osteoclast-specific signalling pathway...
  3. pmc Altering an artificial Gagpolnef polyprotein and mode of ENV co-administration affects the immunogenicity of a clade C HIV DNA vaccine
    Katharina Böckl
    Institute of Medical Microbiology, University of Regensburg, Regensburg, Germany
    PLoS ONE 7:e34723. 2012
    ..These results are guiding the design of ongoing pre-clinical and clinical trials...
  4. pmc The impact of intragenic CpG content on gene expression
    Asli Petra Bauer
    Institute of Medical Microbiology and Hygiene, Molecular Microbiology and Gene Therapy Unit, University of Regensburg, Regensburg, Germany
    Nucleic Acids Res 38:3891-908. 2010
    ..Increased high CpG transcription correlated with changed nucleosomal positions in vitro albeit histone density at the two genes did not change in vivo as monitored by ChIP...
  5. pmc Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
    Sandra Siegert
    Georg Speyer Haus, Institute for Biomedical Research, Paul Ehrlich Strasse 42 44, D 60596 Frankfurt am Main, Germany
    AIDS Res Ther 2:7. 2005
    ..Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry...
  6. ncbi request reprint Correlates of protection, antigen delivery and molecular epidemiology: basics for designing an HIV vaccine
    R Wagner
    Institute of Medical Microbiology and Hygiene University of Regensburg, Germany
    Vaccine 17:1706-10. 1999
    ..A molecular clone of a representative virus strain is now available for the development of innovative antigen delivery systems aiming to be evaluated in future clinical vaccine trials throughout this area...
  7. ncbi request reprint Construction, expression, and immunogenicity of chimeric HIV-1 virus-like particles
    R Wagner
    Institute of Medical Microbiology, University of Regensburg, Germany
    Virology 220:128-40. 1996
    ..These results indicate ways for the rational design and optimal delivery of CTL-stimulating HIV candidate vaccines...
  8. doi request reprint Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design
    D Kosovac
    Molecular Microbiology and Gene Therapy Unit, Institute of Medical Microbiology, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, Germany
    Gene Ther 18:189-98. 2011
    ..5 μg were sufficient to trigger optimal long-term hematologic effects encouraging the use of sequence-optimized transgenes to further reduce effective pDNA amounts...
  9. pmc Multiple effects of codon usage optimization on expression and immunogenicity of DNA candidate vaccines encoding the human immunodeficiency virus type 1 Gag protein
    L Deml
    Institute of Medical Microbiology, University of Regensburg, 93053 Regensburg, Germany
    J Virol 75:10991-1001. 2001
    ..These results suggest that increased Gag expression, rather than modulation of CpG-driven vector immunity, is responsible for the enhanced immunogenicity of the syngag DNA vaccine...
  10. ncbi request reprint Proline residues in the HIV-1 NH2-terminal capsid domain: structure determinants for proper core assembly and subsequent steps of early replication
    T Fitzon
    Institute of Medical Microbiology, Institute of Pathology, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, D 93053, Germany
    Virology 268:294-307. 2000
    ..These results suggest that proline residues in the NH(2)-terminal capsid domain represent critical structure determinants for proper formation of functional virion cores and subsequent stages of early replication...
  11. ncbi request reprint Inhibition of wild-type human immunodeficiency virus and reverse transcriptase inhibitor-resistant variants by Phyllanthus amarus
    F Notka
    CMI Centers for Medical Innovation AG, Fraunhofer Str 15, 82152 Martinsried, Germany
    Antiviral Res 58:175-86. 2003
    ..8 to 14.6 microg/ml. The ability to inhibit replication of a variety of RT inhibitor-resistant HIV-1 strains points to the potential of P. amarus extracts, as natural products, in the chemotherapy of HIV infections...
  12. ncbi request reprint [Status and current strategies of HIV vaccine development]
    J Wild
    Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Regensburg
    Internist (Berl) 44:711-8. 2003
    ..The problems in the development of an HIV-vaccine as well as former and current strategies to overcome these problems are presented here. The current status of human studies of different candidate vaccines is outlined...
  13. ncbi request reprint A new approach to an influenza live vaccine: modification of the cleavage site of hemagglutinin
    J Stech
    Institut fuer Virologie, Klinikum der Philipps Universitaet Marburg, Postfach 2360, 35011 Marburg, Germany
    Nat Med 11:683-9. 2005
    ..At a dose of 10(5) plaque-forming units, it induced complete protection against lethal challenge. This approach allows the conversion of any epidemic strain into a genetically homologous attenuated virus...