Research Topics
| C UngermannSummaryAffiliation: University of Heidelberg Country: Germany Publications
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Detail Information
Publications
A new role for a SNARE protein as a regulator of the Ypt7/Rab-dependent stage of dockingC Ungermann
Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, 3 OG, 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 97:8889-91. 2000..Vam7p provides a functional link between the priming step, which releases it from the cis-SNARE complex, and docking...
Functions of SNAREs in intracellular membrane fusion and lipid bilayer mixingChristian Ungermann
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Cell Sci 118:3819-28. 2005..Recent work indicates that the fusion process itself may comprise two stages and proceed via a hemifusion intermediate...- Control of eukaryotic membrane fusion by N-terminal domains of SNARE proteinsLars E P Dietrich
Biochemie Zentrum Heidelberg BZH, University of Heidelberg, Im Neuenheimer Feld 328, 69120, Heidelberg, Germany
Biochim Biophys Acta 1641:111-9. 2003..Biochemical and genetic experiments as well as the resolution of several NMR and crystal structures of SNARE NTDs shed light on their diverse function... - The N-terminal domain of the t-SNARE Vam3p coordinates priming and docking in yeast vacuole fusionR Laage
University of Heidelberg, Biochemie Zentrum Heidelberg, 69120 Heidelberg, Germany
Mol Biol Cell 12:3375-85. 2001..We conclude that the N-terminus of Vam3p is required for coordination of priming and docking during homotypic vacuole fusion... - The SNARE Ykt6 is released from yeast vacuoles during an early stage of fusionLars E P Dietrich
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
EMBO Rep 6:245-50. 2005..On the basis of these and other findings, we speculate that Ykt6 is released from membranes by depalmitoylation. Such a mechanism could enable recycling of this lipid-anchored SNARE from the vacuole independent of retrograde transport... - The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complexTracy J LaGrassa
Biochemie Zentrum der Universitat Heidelberg, 69120 Heidelberg, Germany
J Cell Biol 168:401-14. 2005..Because Yck3 also promotes efficient vacuole inheritance, we propose that tethering complex phosphorylation is a part of a general, switch-like mechanism for driving changes in organelle architecture... - The SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusionLars E P Dietrich
Biochemie Zentrum Heidelberg BZH, University of Heidelberg, Heidelberg, Germany
EMBO J 23:45-53. 2004..The conservation of Ykt6 and its localization to several organelles suggest that its acyltransferase activity may also be required in other intracellular fusion events... - The transmembrane domain of Vam3 affects the composition of cis- and trans-SNARE complexes to promote homotypic vacuole fusionJan Rohde
Interdisziplinäres Zentrum für Neurowissenschaften, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany
J Biol Chem 278:1656-62. 2003..Finally, palmitoylation of Vac8, a reaction that also occurs early during priming is reduced by almost one-half. Since palmitoylated Vac8 is required beyond trans-SNARE complex formation, this may partially explain the fusion deficiency... - A cycle of Vam7p release from and PtdIns 3-P-dependent rebinding to the yeast vacuole is required for homotypic vacuole fusionChristine Boeddinghaus
Biochemie-Zentrum Heidelberg, University of Heidelberg, 69120 Heidelberg, Germany
J Cell Biol 157:79-89. 2002..We propose that the Vam7p cycle is linked to the availability of a lipid domain on yeast vacuoles, which is essential for coordinating the fusion reaction prior to and beyond docking... - Palmitoylation determines the function of Vac8 at the yeast vacuoleKanagaraj Subramanian
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Cell Sci 119:2477-85. 2006..Our data suggest that palmitoylation is not a mere hydrophobic anchor required solely for localization, but influences the protein function(s)... - The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8Haitong Hou
Biochemie Zentrum der Universitat Heidelberg, Germany
Proc Natl Acad Sci U S A 102:17366-71. 2005..Our data indicate that Pfa3 has an important but not exclusive function for Vac8 localization to the vacuole... - ATP-independent control of Vac8 palmitoylation by a SNARE subcomplex on yeast vacuolesLars E P Dietrich
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Biol Chem 280:15348-55. 2005..We speculate that during vacuole fusion, Nyv1 is the classical R-SNARE, whereas the Ykt6-containing complex has a novel function in Vac8 palmitoylation... - On the mechanism of protein palmitoylationLars E P Dietrich
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
EMBO Rep 5:1053-7. 2004..In this review, we discuss these observations in the context of a potential general mechanism of acylation... - Expanding dynamin: from fission to fusionKarolina Peplowska
Nat Cell Biol 7:103-4. 2005 - Longins and their longin domains: regulated SNAREs and multifunctional SNARE regulatorsValeria Rossi
Molecular Biology and Bioinformatics Unit MOLBINFO, Department of Biology, University of Padua, 35131 Padua, Italy
Trends Biochem Sci 29:682-8. 2004..In mammals, TI-VAMP and Ykt6 are crucial for neuronal function, and defects in longin structure or function might underlie some human neurological pathologies... - Self-interaction of a SNARE transmembrane domain promotes the hemifusion-to-fusion transitionMathias W Hofmann
Lehrstuhl Chemie der Biopolymere, Technische Universitat Munchen, Weihenstephaner Berg 3, 85354 Freising, Germany
J Mol Biol 364:1048-60. 2006..Since covalent dimerisation of this mutant recovered wild-type behaviour, homodimerisation of a SNARE transmembrane domain appears to control the transition of a hemifusion intermediate to complete lipid mixing... 
The vacuolar V1/V0-ATPase is involved in the release of the HOPS subunit Vps41 from vacuoles, vacuole fragmentation and fusionKozue Takeda
Department of Biology, University of Osnabruck, Barbarastrasse 13, 49076 Osnabruck, Germany
FEBS Lett 582:1558-63. 2008..Our data suggest a connection between vacuole biogenesis and membrane fusion...- Farnesylation of the SNARE protein Ykt6 increases its stability and helical foldingOlena Pylypenko
Abteilung Physikalische Biochemie, Max Planck Institut fur molekulare Physiologie, Otto Hahn Str 11, 44227 Dortmund, Germany
J Mol Biol 377:1334-45. 2008..Together, these observations support a previously suggested closed conformation of cytosolic Ykt6, where the C-terminal farnesyl moiety folds onto a hydrophobic groove in the N-terminal longin domain... - Yeast vacuole fusion: a model system for eukaryotic endomembrane dynamicsClemens W Ostrowicz
Department of Biology, Biochemistry Section, University of Osnabruck, Osnabruck, Germany
Autophagy 4:5-19. 2008..A number of fusion factors were identified and characterized over the last several years, and placed into the fusion cascade. Within this review, we will present and discuss the current state of our knowledge on vacuole fusion... - The CORVET tethering complex interacts with the yeast Rab5 homolog Vps21 and is involved in endo-lysosomal biogenesisKarolina Peplowska
University of Osnabruck, Department of Biology, Biochemistry Section, Barbarastrasse 13, 49076 Osnabruck, Germany
Dev Cell 12:739-50. 2007..Our data suggest that modular assembled tethering complexes define organelle biogenesis in the endocytic pathway... - Rab cascades and tethering factors in the endomembrane systemDaniel F Markgraf
University of Osnabruck, Department of Biology, Biochemistry Section, Barbarastrasse 13, 49076 Osnabruck, Germany
FEBS Lett 581:2125-30. 2007..Recent studies suggest that Rabs are linked to each other via their effectors, thus coordinating protein transport in the endomembrane system. Within this review, we will focus on selected examples that highlight these issues... - Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiaeNils J Faergeman
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
Biochem J 380:907-18. 2004..We suggest that the reduced ceramide synthesis in Acb1p-depleted cells leads to severely altered vacuole morphology, perturbed vacuole assembly and strong inhibition of homotypic vacuole fusion... - UVRAG reveals its second natureKarolina Peplowska
Department of Biology, University of Osnabruck, Barbarastrasse 13, 49076 Osnabruck, Germany
Nat Cell Biol 10:759-61. 2008..UVRAG, a known regulator of autophagosome formation, also promotes autophagosome maturation by recruiting the fusion machinery of the late endosome...