Günter Speit

Summary

Affiliation: University of Ulm
Country: Germany

Publications

  1. doi request reprint The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair
    Günter Speit
    Institut fur Humangenetik, Universitat Ulm, Ulm, Germany
    Methods Mol Biol 920:79-90. 2012
  2. ncbi request reprint The low molecular weight DNA diffusion assay as an indicator of cytotoxicity for the in vitro comet assay
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 29:267-77. 2014
  3. ncbi request reprint Investigations on potential co-mutagenic effects of formaldehyde
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, 89069 Ulm, Germany Electronic address
    Mutat Res Fundam Mol Mech Mutagen 760:48-56. 2014
  4. doi request reprint Genotoxic effects of exposure to radiofrequency electromagnetic fields (RF-EMF) in HL-60 cells are not reproducible
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 755:163-6. 2013
  5. doi request reprint Does the recommended lymphocyte cytokinesis-block micronucleus assay for human biomonitoring actually detect DNA damage induced by occupational and environmental exposure to genotoxic chemicals?
    Günter Speit
    Institut fur Humangenetik, Universitat Ulm, D 89069 Ulm, Germany
    Mutagenesis 28:375-80. 2013
  6. doi request reprint Hyperthermia-induced genotoxic effects in human A549 cells
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, 89069 Ulm, Germany
    Mutat Res 747:1-5. 2013
  7. doi request reprint Insensitivity of the in vitro cytokinesis-block micronucleus assay with human lymphocytes for the detection of DNA damage present at the start of the cell culture
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 27:743-7. 2012
  8. doi request reprint Further investigations on the modified comet assay for measuring aphidicolin-block nucleotide excision repair
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 28:145-51. 2013
  9. doi request reprint Re-evaluation of a reported increased micronucleus frequency in lymphocytes of workers occupationally exposed to formaldehyde
    Günter Speit
    Institut fur Humangenetik, Universitat Ulm, Ulm, Germany
    Mutat Res 744:161-6. 2012
  10. doi request reprint Inhalation of formaldehyde does not induce systemic genotoxic effects in rats
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 677:76-85. 2009

Collaborators

Detail Information

Publications67

  1. doi request reprint The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair
    Günter Speit
    Institut fur Humangenetik, Universitat Ulm, Ulm, Germany
    Methods Mol Biol 920:79-90. 2012
    ..This chapter describes a standard protocol of the alkaline comet assay and points to some useful modifications...
  2. ncbi request reprint The low molecular weight DNA diffusion assay as an indicator of cytotoxicity for the in vitro comet assay
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 29:267-77. 2014
    ..Measurements of cytotoxicity by these methods only at an early preparation time after exposure to genotoxic substances may lead to erroneous results. ..
  3. ncbi request reprint Investigations on potential co-mutagenic effects of formaldehyde
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, 89069 Ulm, Germany Electronic address
    Mutat Res Fundam Mol Mech Mutagen 760:48-56. 2014
    ..The results of these experiments do not support the assumption that low FA concentrations might interfere with the repair of DNA damage induced by other mutagens. ..
  4. doi request reprint Genotoxic effects of exposure to radiofrequency electromagnetic fields (RF-EMF) in HL-60 cells are not reproducible
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 755:163-6. 2013
    ..We could not find an explanation for the conflicting results. However, the negative repeat experiments suggest that the biological significance of genotoxic effects of RF-EMF reported by the REFLEX study should be re-assessed. ..
  5. doi request reprint Does the recommended lymphocyte cytokinesis-block micronucleus assay for human biomonitoring actually detect DNA damage induced by occupational and environmental exposure to genotoxic chemicals?
    Günter Speit
    Institut fur Humangenetik, Universitat Ulm, D 89069 Ulm, Germany
    Mutagenesis 28:375-80. 2013
    ..Thus, it seems worthwhile to reconsider the use of the CBMN assay as presently conducted for the detection of genotoxic exposure in human biomonitoring...
  6. doi request reprint Hyperthermia-induced genotoxic effects in human A549 cells
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, 89069 Ulm, Germany
    Mutat Res 747:1-5. 2013
    ..Cytotoxic effects occurred in parallel to the genotoxic effects but apoptosis was not significantly induced under these experimental conditions...
  7. doi request reprint Insensitivity of the in vitro cytokinesis-block micronucleus assay with human lymphocytes for the detection of DNA damage present at the start of the cell culture
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 27:743-7. 2012
    ..Potential consequences for the interpretation of human biomonitoring studies are discussed in this article...
  8. doi request reprint Further investigations on the modified comet assay for measuring aphidicolin-block nucleotide excision repair
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 28:145-51. 2013
    ..Altogether, our results suggest that there is still need for standardisation and validation of this assay before it can be reliably used in human biomonitoring studies...
  9. doi request reprint Re-evaluation of a reported increased micronucleus frequency in lymphocytes of workers occupationally exposed to formaldehyde
    Günter Speit
    Institut fur Humangenetik, Universitat Ulm, Ulm, Germany
    Mutat Res 744:161-6. 2012
    ..Possible implications for the use of the CBMNT in human biomonitoring studies are discussed...
  10. doi request reprint Inhalation of formaldehyde does not induce systemic genotoxic effects in rats
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 677:76-85. 2009
    ..It can be concluded that inhalation of FA in a 28-day study with FA concentrations up to 15 ppm does not lead to systemic genotoxic effects in the blood of rats...
  11. ncbi request reprint Local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated epithelial cells
    Günter Speit
    Universitat Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutat Res 613:1-9. 2006
    ..On the basis of the available data, it is not yet possible to assess the local genotoxicity of FA in humans and to draw meaningful conclusions with regard to a dose-effect relationship for risk estimation...
  12. ncbi request reprint Genotoxic effects of exposure to radiofrequency electromagnetic fields (RF-EMF) in cultured mammalian cells are not independently reproducible
    Günter Speit
    Universitat Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutat Res 626:42-7. 2007
    ..The reasons for the difference between the results reported by the REFLEX project and our experiments remain unclear...
  13. ncbi request reprint Assessment of local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated buccal mucosa cells
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89070 Ulm, Germany
    Mutat Res 627:129-35. 2007
    ..This study, which was performed under GLP-like conditions, clearly indicates that FA does not induce MN in buccal mucosa cells after peak exposures up to 1 ppm and a cumulative exposure of 13.5 ppm h over 2 weeks...
  14. ncbi request reprint Characterization of the genotoxic potential of formaldehyde in V79 cells
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 22:387-94. 2007
    ....
  15. ncbi request reprint The genotoxic potential of glutaraldehyde in mammalian cells in vitro in comparison with formaldehyde
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, Ulm, Germany
    Mutat Res 649:146-54. 2008
    ..However, the differences in genotoxicity cannot readily explain the different carcinogenic activities of the two compounds...
  16. doi request reprint The human lung cell line A549 does not develop adaptive protection against the DNA-damaging action of formaldehyde
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, Ulm, Germany
    Environ Mol Mutagen 51:130-7. 2010
    ..Neither metabolic inactivation of FA nor the repair of FA-induced DPX seems to be enhanced in cells pretreated with FA...
  17. doi request reprint Genotoxic effects of formaldehyde in the human lung cell line A549 and in primary human nasal epithelial cells
    Günter Speit
    Institut fur Humangenetik, Universitat Ulm, Ulm, Germany
    Environ Mol Mutagen 49:300-7. 2008
    ....
  18. doi request reprint How to assess the mutagenic potential of cosmetic products without animal tests?
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, Oberer Eselsberg M25, D 89069 Ulm, Germany
    Mutat Res 678:108-12. 2009
    ....
  19. doi request reprint Low sensitivity of the comet assay to detect acetaldehyde-induced genotoxicity
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 657:93-7. 2008
    ..2 and 0.5 mM, respectively). These results suggest that the comet assay has a low sensitivity for the detection of AA-induced DNA lesions leading to the induction of SCE and MN...
  20. doi request reprint The effect of inhibited replication on DNA migration in the comet assay in relation to cytotoxicity and clastogenicity
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 655:22-7. 2008
    ..Furthermore, the in vitro comet assay seems to be less sensitive towards this type of secondary DNA effects than the in vitro chromosome aberration test...
  21. doi request reprint The comet assay as an indicator test for germ cell genotoxicity
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 681:3-12. 2009
    ..However, studies to standardize and validate these methods are necessary before the comet assay can be usefully applied in risk assessment of germ cell mutagens...
  22. doi request reprint The in vivo or ex vivo origin of micronuclei measured in human biomonitoring studies
    Günter Speit
    Institut fur Humangenetik, Universitat Ulm, D 89069 Ulm, Germany
    Mutagenesis 26:107-10. 2011
    ....
  23. doi request reprint Does formaldehyde induce aneuploidy?
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 26:805-11. 2011
    ..Altogether, our results confirm the clastogenicity of FA in cultured mammalian cells but exclude a significant aneugenic activity...
  24. ncbi request reprint The implausibility of systemic genotoxic effects measured by the comet assay in rats exposed to formaldehyde
    Günter Speit
    Abteilung Humangenetik, Universitat Ulm, D 89069 Ulm, Germany
    J Proteome Res 5:2523-4. 2006
    ..These three aspects are critically discussed, and the plausibility of the results published by Im and colleagues is questioned...
  25. doi request reprint Analysis of micronuclei, histopathological changes and cell proliferation in nasal epithelium cells of rats after exposure to formaldehyde by inhalation
    Günter Speit
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 721:127-35. 2011
    ..Because only limited experience exists for the MNT with rat nasal epithelial cells, this result has to be interpreted with great care. The contribution of mutagenicity to FA's carcinogenicity in rat nasal epithelium remains unclear...
  26. doi request reprint Assessment of genotoxic effects and changes in gene expression in humans exposed to formaldehyde by inhalation under controlled conditions
    Jasmin Zeller
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutagenesis 26:555-61. 2011
    ..Inhalation of FA did also not cause alterations in the expression of genes in a microarray analysis with nasal biopsies and peripheral blood cells...
  27. doi request reprint Hemodynamic, metabolic, and organ function effects of pure oxygen ventilation during established fecal peritonitis-induced septic shock
    Balázs Hauser
    Sektion Anasthesiologische Pathophysiologie und Verfahrensentwicklung, Klinik fur Anasthesiologie, Universitatsklinikum, Ulm, Germany
    Crit Care Med 37:2465-9. 2009
    ..To test the hypothesis whether pure oxygen ventilation is equally safe and beneficial in fully developed fecal peritonitis-induced septic shock as hyperoxia initiated at the induction of sepsis...
  28. ncbi request reprint The parp-1 inhibitor ino-1001 facilitates hemodynamic stabilization without affecting DNA repair in porcine thoracic aortic cross-clamping-induced ischemia/reperfusion
    Balázs Hauser
    Sektion Anasthesiologische Pathophysiologie und Verfahrensentwicklung, Universitatsklinikum, Ulm, Germany
    Shock 25:633-40. 2006
    ..The reduced norepinephrine requirements during reperfusion suggest a positive inotropic effect of INO-1001, as demonstrated by other authors. In our model, INO-1001 proved to be safe with respect to DNA repair...
  29. ncbi request reprint Overexpression of heme oxygenase-1 (HO-1) in V79 cells results in increased resistance to hyperbaric oxygen (HBO)-induced DNA damage
    Andreas Rothfuss
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Environ Mol Mutagen 40:258-65. 2002
    ..Since the observed protective effects were abolished by cotreatment with the HO-1 inhibitor tin-mesoporphyrin, our study suggests that a low-level overexpression of HO-1 provides protection against oxidative DNA damage induced by HBO...
  30. doi request reprint Inhalation of formaldehyde does not induce genotoxic effects in broncho-alveolar lavage (BAL) cells of rats
    Simone Neuss
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 695:61-8. 2010
    ..The results of our inhalation study, which was performed under GLP conditions, call into question the biological significance of previously reported genotoxic effects in the lung of rats after FA inhalation...
  31. doi request reprint Effects of intravenous sulfide during porcine aortic occlusion-induced kidney ischemia/reperfusion injury
    Florian Simon
    Sektion Anasthesiologische Pathophysiologie und Verfahrensentwicklung, Klinik fur Anasthesiologie, Universitatsklinikum, Ulm, Germany
    Shock 35:156-63. 2011
    ..The higher nuclear transcription factor κB activation was probably due to the drop in temperature...
  32. ncbi request reprint Characterization of formaldehyde's genotoxic mode of action by gene expression analysis in TK6 cells
    Stefanie Kuehner
    Institut fur Humangenetik, Universitat Ulm, Albert Einstein Allee 11, 89081, Ulm, Germany
    Arch Toxicol 87:1999-2012. 2013
    ..Exposure of TK6 cells to FA led to a discrete gene expression pattern, and all toxicogenomics analyses revealed a closer relationship of FA with clastogens than with aneugens...
  33. ncbi request reprint Radiosensitivity of lymphoblastoid cell lines with a heterozygous BRCA1 mutation is not detected by the comet assay and pulsed field gel electrophoresis
    Kristina Trenz
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutagenesis 20:131-7. 2005
    ..However, these results do not generally exclude DNA-DSB repair deficiency in these cell lines because the methods applied have limited sensitivity and only measure the speed but not the fidelity of the repair process...
  34. doi request reprint Is individual nasal sensitivity related to cellular metabolism of formaldehyde and susceptibility towards formaldehyde-induced genotoxicity?
    Jasmin Zeller
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Mutat Res 723:11-7. 2011
    ..There was also no close correlation between the various indicators of cellular sensitivity towards FA-induced genotoxic effects and no subgroups were identified with particular mutagen sensitivity towards FA...
  35. ncbi request reprint Investigations on the mechanism of hyperbaric oxygen (HBO)-induced adaptive protection against oxidative stress
    Andreas Rothfuss
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutat Res 508:157-65. 2002
    ....
  36. doi request reprint Investigations of potential susceptibility toward formaldehyde-induced genotoxicity
    Jasmin Zeller
    Institut fur Humangenetik, Universitat Ulm, 89069 Ulm, Germany
    Arch Toxicol 86:1465-73. 2012
    ..These results suggest that a low scaling factor to address possible human inter-individual differences in FA-induced genotoxicity could be reasonable...
  37. ncbi request reprint Enhancement of genotoxic effects in the comet assay with human blood samples by aphidicolin
    Günter Speit
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Toxicol Lett 153:303-10. 2004
    ..However, for human biomonitoring, a sensitive protocol still has to be established...
  38. doi request reprint Gene expression changes in primary human nasal epithelial cells exposed to formaldehyde in vitro
    Simone Neuss
    Universitat Ulm, Institut fur Humangenetik, D 89069 Ulm, Germany
    Toxicol Lett 198:289-95. 2010
    ..Interestingly, altered expression of genes involved in the main pathways for FA detoxification and the repair of DPX were not specifically detected...
  39. ncbi request reprint Genotoxic effects induced by formaldehyde in human blood and implications for the interpretation of biomonitoring studies
    Oliver Schmid
    Institut fur Humangenetik, Universitat Ulm, D 89069 Ulm, Germany
    Mutagenesis 22:69-74. 2007
    ..Our results characterize the genotoxic potential of FA in cultured lymphocytes and lead to the conclusion that cytogenetic effects of FA are very unlikely to occur in blood cultures of FA-exposed subjects...
  40. doi request reprint Exposure of human nasal epithelial cells to formaldehyde does not lead to DNA damage in lymphocytes after co-cultivation
    Simone Neuss
    Institut fur Humangenetik, Universitat Ulm, Ulm, Germany
    Mutagenesis 25:359-64. 2010
    ....
  41. ncbi request reprint Assessment of DNA damage in peripheral blood of heavy smokers with the comet assay and the micronucleus test
    Heike Hoffmann
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutat Res 581:105-14. 2005
    ..Baseline and gamma radiation-induced micronucleus frequencies were determined. None of these approaches revealed a significant difference between heavy smokers and non-smokers with regard to a genotoxic effect in peripheral blood cells...
  42. ncbi request reprint Oxygenated water does not induce genotoxic effects in the comet assay
    Günter Speit
    Universitatsklinikum Ulm, Abteilung Humangenetik, Albert Einstein Allee 11, Germany
    Toxicol Lett 133:203-10. 2002
    ..However, both the in vivo and the in vitro studies with the comet assay in the absence and presence of FPG post-treatment did not provide evidence for a genotoxic effect of oxygenated water...
  43. doi request reprint Micronuclei in peripheral blood from patients after cytostatic therapy mainly arise ex vivo from persistent damage
    Nicole Sibel Arsoy
    Institut fur Humangenetik, Universitat Ulm, Germany
    Mutagenesis 24:351-7. 2009
    ....
  44. ncbi request reprint Enhanced sensitivity of peripheral blood lymphocytes from women carrying a BRCA1 mutation towards the mutagenic effects of various cytostatics
    Kristina Trenz
    Universitatsklinikum Ulm, Abteilung Humangenetik, Ulm D 89070, Germany
    Mutat Res 544:279-88. 2003
    ....
  45. doi request reprint The effect of superoxide dismutase overexpression on hepatic gluconeogenesis and whole-body glucose oxidation during resuscitated normotensive murine septic shock
    Vladislava Simkova
    Sektion Anasthesiologische Pathophysiologie und Verfahrensentwicklung, Universitatsklinikum, Ulm, Germany
    Shock 30:578-84. 2008
    ..v. norepinephrine...
  46. ncbi request reprint Mutagen sensitivity of peripheral blood from women carrying a BRCA1 or BRCA2 mutation
    Kristina Trenz
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutat Res 500:89-96. 2002
    ....
  47. ncbi request reprint The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair
    Günter Speit
    Abteilung Humangenetik, Universitatsklinikum Ulm, Germany
    Methods Mol Biol 314:275-86. 2006
    ..This chapter describes a standard protocol of the alkaline comet assay and points to some useful modifications...
  48. ncbi request reprint Genotoxic and protective effects of hyperbaric oxygen in A549 lung cells
    Günter Speit
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutagenesis 18:545-8. 2003
    ..Our study indicates that A549 cells are an appropriate cell culture system for the evaluation of genetic effects induced by HBO in lung cells...
  49. ncbi request reprint Investigations on the effect of cigarette smoking in the comet assay
    Günter Speit
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070, Ulm, Germany
    Mutat Res 542:33-42. 2003
    ..Although more data is needed for a final conclusion, this study indicates some limitations of the comet assay with regard to the detection of DNA damage induced by environmental mutagens in peripheral blood cells...
  50. ncbi request reprint Sensitivity of the FPG protein towards alkylation damage in the comet assay
    Günter Speit
    Abteilung Humangenetik, Universitatsklinikum Ulm, D 89070 Ulm, Germany
    Toxicol Lett 146:151-8. 2004
    ..Furthermore, reducing the pH leads to a considerable loss in sensitivity and should not be used in biomonitoring and other applications which require a sensitive protocol...
  51. doi request reprint Further characterization of the genotoxicity of formaldehyde in vitro by the sister chromatid exchange test and co-cultivation experiments
    Simone Neuss
    Institut fur Humangenetik, Universitat Ulm, D 89069 Ulm, Germany
    Mutagenesis 23:355-7. 2008
    ..However, FA that has entered a cell is not released and does not damage other cells. Possible implications for the mutagenicity of FA in vivo will be discussed...
  52. ncbi request reprint Genetic polymorphisms and the effect of cigarette smoking in the comet assay
    Heike Hoffmann
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutagenesis 20:359-64. 2005
    ..An independent second evaluation was carried out for all experiments. None of these approaches revealed a significant difference between nonsmokers and smokers...
  53. ncbi request reprint The effect of smoking on DNA effects in the comet assay: a meta-analysis
    Heike Hoffmann
    Universitat Ulm, Abteilung Humangenetik, Ulm, Germany
    Mutagenesis 20:455-66. 2005
    ....
  54. ncbi request reprint Mutagen sensitivity of human lymphoblastoid cells with a BRCA1 mutation
    Kristina Trenz
    Universitatsklinikum Ulm, Abteilung Humangenetik, Ulm, Germany
    Breast Cancer Res Treat 78:69-79. 2003
    ..Therefore, the use of LCL to study the mechanisms underlying mutagen sensitivity due to a heterozygous BRCA1 mutation seems to be limited...
  55. ncbi request reprint Genotoxicity of hyperbaric oxygen
    Günter Speit
    Abteilung Humangenetik, Universitatsklinikum Ulm, Albert Einstein Allee 11, D 89070, Ulm, Germany
    Mutat Res 512:111-9. 2002
    ..Furthermore, since an induction of anti-oxidative defense mechanisms has been determined after HBO exposure, a modified treatment regimen of HBO therapy is proposed which avoids genotoxic effects...
  56. ncbi request reprint Evaluation of mutagenic effects of formaldehyde in vitro: detection of crosslinks and mutations in mouse lymphoma cells
    Günter Speit
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutagenesis 17:183-7. 2002
    ..Our results indicate that small-scale chromosomal rearrangements (e.g. deletions or recombinations) are mainly involved in FA-induced mutagenesis in the MLA...
  57. ncbi request reprint Targeted gene correction of hprt mutations by 45 base single-stranded oligonucleotides
    Oliver Kenner
    Department of Human Genetics, University of Ulm, Albert Einstein Allee 11, D 89070 Ulm, Germany
    Biochem Biophys Res Commun 299:787-92. 2002
    ..The correction efficiency was very low but reproducible. We suggest that this system allows one to investigate targeted gene correction in dependence on the target sequence and the oligonucleotides used...
  58. ncbi request reprint The comet assay: a sensitive genotoxicity test for the detection of DNA damage
    Günter Speit
    Abteilung Humangenetik, Universitätklinikum Ulm, Germany
    Methods Mol Biol 291:85-95. 2005
    ..Applications of the comet assay for detecting DNA damage in individual cells are briefly reviewed...
  59. ncbi request reprint Appropriate in vitro test conditions for genotoxicity testing of fibers
    Günter Speit
    Universitatsklinikum Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Inhal Toxicol 14:79-90. 2002
    ..Further investigations are needed to shown whether this approach can be recommended for genotoxicity testing of fibers in general...
  60. doi request reprint Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury
    Florian Simon
    Sektion Anasthesiologische Pathophysiologie und Verfahrensentwicklung, Abteilung für Thorax und Gefäbetachirurgie, Universitatsklinikum, Ulm, Germany
    Shock 30:359-64. 2008
    ..Investigations are warranted, hence, whether it may also protect other organs after I/R injury...
  61. ncbi request reprint Effects of ventilation with 100% oxygen during early hyperdynamic porcine fecal peritonitis
    Eberhard Barth
    Sektion Anasthesiologische Pathophysiologie und Verfahrensentwicklung, Universitatsklinikum, Ulm, Germany
    Crit Care Med 36:495-503. 2008
    ..On the other hand, oxygen breathing improved survival in various shock models. Therefore, we hypothesized that hyperoxia may be beneficial during early septic shock...
  62. doi request reprint Genetic polymorphisms in the formaldehyde dehydrogenase gene and their biological significance
    Walter Just
    Universitat Ulm, Institut fur Humangenetik, Ulm, Germany
    Toxicol Lett 207:121-7. 2011
    ..Altogether, our study did not identify biologically relevant polymorphisms in transcribed regions of the FDH gene, which may lead to inter-individual differences in the metabolic inactivation of FA...
  63. ncbi request reprint Influence of an orally effective SOD on hyperbaric oxygen-related cell damage
    Claus M Muth
    Sektion Anasthesiologische Pathophysiologie und Verfahrensentwicklung, Universitatsklinikum Ulm, D 89073 Ulm, Germany
    Free Radic Res 38:927-32. 2004
    ..045). We conclude that an orally effective SOD-wheat gliadin mixture is able to protect against DNA damage, which coincided with reduced blood isoprostane levels, and may therefore be used as an antioxidant...
  64. ncbi request reprint Concurrent targeted exchange of three bases in mammalian hprt by oligonucleotides
    Oliver Kenner
    Department of Human Genetics, University of Ulm, D 89070 Ulm, Germany
    Biochem Biophys Res Commun 321:1017-23. 2004
    ..These results indicate that single-stranded oligonucleotides can generate two or three nucleotide exchanges in a mammalian chromosomal gene...
  65. doi request reprint Analysis of leukemia-specific aneuploidies in cultured myeloid progenitor cells in the absence and presence of formaldehyde exposure
    Stefanie Kuehner
    Institut fur Humangenetik, Universitat Ulm, D 89081 Ulm, Germany
    Toxicol Sci 128:72-8. 2012
    ..Our results do not support the assumption of a specific effect of FA on myeloid progenitor cells as a potential mechanism for the induction of leukemia...
  66. ncbi request reprint Effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping
    Jochen Kick
    Abteilung Thorax und Gefässchirurgie, Universitatsklinikum Ulm, Steinhovelstrasse 9, 89075, Ulm, Germany
    Intensive Care Med 33:694-702. 2007
    ....
  67. ncbi request reprint Review of the in vivo genotoxicity tests performed with styrene
    Günter Speit
    Abteilung Humangenetik, Universitatsklinikum Ulm, D 89070 Ulm, Germany
    Mutat Res 589:67-79. 2005
    ..However, there is no clear evidence that styrene induces mutagenic/clastogenic effects in vivo when tested under appropriate test conditions...