Affiliation: University of Heidelberg
Kempf G, Wild K, Sinning I. Structure of the complete bacterial SRP Alu domain. Nucleic Acids Res. 2014;42:12284-94 pubmed publisher
..Our findings provide the structural basis for purely RNA-driven elongation arrest in prokaryotes, and give insights into the structural adaption of SRP RNA during evolution. ..
Kempf G, Stjepanovic G, Sloan J, Hendricks A, Lapouge K, Sinning I. The Escherichia coli SRP Receptor Forms a Homodimer at the Membrane. Structure. 2018;26:1440-1450.e5 pubmed publisher
..Our findings add to the current understanding of SRP GTPases and may challenge previous studies that did not consider homodimerization of FtsY. ..
Kharde S, CalviÃ±o F, Gumiero A, Wild K, Sinning I. The structure of Rpf2-Rrs1 explains its role in ribosome biogenesis. Nucleic Acids Res. 2015;43:7083-95 pubmed publisher
..Our data provide a structural model for 5S RNP recruitment to the pre-60S particle and explain why removal of Rpf2-Rrs1 is necessary for rearrangements to drive 60S maturation. ..
Wild K, Bange G, Motiejunas D, Kribelbauer J, Hendricks A, Segnitz B, et al
. Structural Basis for Conserved Regulation and Adaptation of the Signal Recognition Particle Targeting Complex. J Mol Biol. 2016;428:2880-97 pubmed publisher
..Structural analyses of the GDP-bound, post-hydrolysis states reveal a conserved, magnesium-sensitive switch within the I-box. Overall, we provide a general model for SRP cycle regulation by RNA. ..
Hackmann Y, Joedicke L, Panneels V, Sinning I. Expression of membrane proteins in the eyes of transgenic Drosophila melanogaster. Methods Enzymol. 2015;556:219-39 pubmed publisher
..The protocols provided here are robust and straightforward to follow even without prior experience in Drosophila work. ..
Jadhav B, Wild K, Pool M, Sinning I. Structure and Switch Cycle of SRÎ² as Ancestral Eukaryotic GTPase Associated with Secretory Membranes. Structure. 2015;23:1838-1847 pubmed publisher
..We define the GTPase switch cycle of SRÎ² and identify specific differences to the Arf and Sar1 families with implications for GTPase regulation. Our data allow a better integration of SRÎ² into the scheme of protein targeting. ..
Thoms M, Ahmed Y, Maddi K, Hurt E, Sinning I. Concerted removal of the Erb1-Ytm1 complex in ribosome biogenesis relies on an elaborate interface. Nucleic Acids Res. 2016;44:926-39 pubmed publisher
..Under these mutant conditions, it becomes clear that an intact Erb1-Ytm1 complex is required for 60S maturation and that loss of this stable interaction prevents ribosome production. ..
Becker M, Lapouge K, Segnitz B, Wild K, Sinning I. Structures of human SRP72 complexes provide insights into SRP RNA remodeling and ribosome interaction. Nucleic Acids Res. 2017;45:470-481 pubmed publisher
..Docking of the S domain structure into cryo-electron microscopy density maps reveals multiple contact sites between SRP68/72 and the ribosome, and explains the role of SRP72 in the SRP pathway. ..
van Noort V, Bradatsch B, Arumugam M, Amlacher S, Bange G, Creevey C, et al
. Consistent mutational paths predict eukaryotic thermostability. BMC Evol Biol. 2013;13:7 pubmed publisher
..The comparative analysis of these three genomes not only enhances our understanding of the evolution of thermophily, but also provides new ways to engineer protein stability. ..