Genomes and Genes
Affiliation: University of Essen
- Carbohydrate metabolism in Thermoproteus tenax: in vivo utilization of the non-phosphorylative Entner-Doudoroff pathway and characterization of its first enzyme, glucose dehydrogenaseB Siebers
FB 9 Mikrobiologie, Universität GH Essen, Universitatsstrasse 5, D 45117 Essen, Germany
Arch Microbiol 168:120-7. 1997....
- Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax by use of genomic and biochemical dataBettina Siebers
Department of Microbiology, Universitat Duisburg Essen, 45117 Essen, Germany
J Bacteriol 186:2179-94. 2004..Almost all genes necessary for glycogen and trehalose metabolism were identified in the T. tenax genome...
- Archaeal fructose-1,6-bisphosphate aldolases constitute a new family of archaeal type class I aldolaseB Siebers
Department of Microbiology, Universitat Essen, 45117 Essen, Germany
J Biol Chem 276:28710-8. 2001..Because this new family shows no significant sequence similarity to classical Class I and II enzymes, a new name is proposed, archaeal type Class I FBP aldolases (FBP aldolase Class IA)...
- PPi-dependent phosphofructokinase from Thermoproteus tenax, an archaeal descendant of an ancient line in phosphofructokinase evolutionB Siebers
FB 9 Mikrobiologie, Universität GH Essen, Germany
J Bacteriol 180:2137-43. 1998..tenax and Amycolatopsis methanolica and the ATP-PFK from Streptomyces coelicolor. Comparative analyses of the pattern of conserved active-site residues strongly suggest that the group III PFKs originally bound PPi as a cosubstrate...
- Pyruvate kinase of the hyperthermophilic crenarchaeote Thermoproteus tenax: physiological role and phylogenetic aspectsA Schramm
Department of Microbiology, Universität GH Essen, D 45117 Essen, Germany
J Bacteriol 182:2001-9. 2000....
- NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase from Thermoproteus tenax. The first identified archaeal member of the aldehyde dehydrogenase superfamily is a glycolytic enzyme with unusual regulatory propertiesN A Brunner
Department of Microbiology, FB 9, Universit t GH Essen, Universit tsstrasse 5, 45117 Essen, Germany
J Biol Chem 273:6149-56. 1998..tenax. Its regulatory properties substitute for those lacking in the reversible nonregulated pyrophosphate-dependent phosphofructokinase in this variant of the Embden-Meyerhof-Parnas pathway...
- Embden-Meyerhof-Parnas and Entner-Doudoroff pathways in Thermoproteus tenax: metabolic parallelism or specific adaptation?H Ahmed
Department of Microbiology, University of Duisburg Essen, Universitatsstrasse 5, 45117 Essen, Germany
Biochem Soc Trans 32:303-4. 2004..An overview of the three different pathways is presented and the physiological function of the variants is discussed...
- Role of two different glyceraldehyde-3-phosphate dehydrogenases in controlling the reversible Embden-Meyerhof-Parnas pathway in Thermoproteus tenax: regulation on protein and transcript levelN A Brunner
Department of Microbiology, Universität GH Essen, Germany
Extremophiles 5:101-9. 2001..The different regulation of the two counteracting glyceraldehyde-3-phosphate dehydrogenases is discussed with respect to the flux control of the T. tenax-specific EMP variant...
- Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classesDaniel Kehrer
Department of Biology and Geography, Institute of Biology, Microbiology I, Universitat Duisburg Essen, Universitatsstr, 5, 45117 Essen, Germany
BMC Genomics 8:301. 2007..In the T. tenax genome, a gene homolog with similarity to putative hydroxypyruvate reductase/glycerate dehydrogenase and glycerate kinase was identified...
- DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenaxMelanie Zaparty
Department of Biology and Geography, Institute of Biology, Microbiology I, University of Duisburg Essen, Universitätsstr 5, 45117 Essen, Germany
J Bacteriol 190:2231-8. 2008..A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO2-H2 was performed...
- Crystal structure and stereochemical studies of KD(P)G aldolase from Thermoproteus tenaxAnuschka Pauluhn
Swiss Light Source, Paul Scherrer Institut, CH 5232 Villigen PSI, Switzerland
Proteins 72:35-43. 2008....
- A novel trehalose synthesizing pathway in the hyperthermophilic Crenarchaeon Thermoproteus tenax: the unidirectional TreT pathwayTheresa Kouril
Department of Chemistry, Biofilm Centre, Molecular Enzyme Technology and Biochemistry, University of Duisburg Essen, Lotharstr, MF Building, 47057 Duisburg, Germany
Arch Microbiol 190:355-69. 2008..Here we present a new pathway exclusively involved in trehalose synthesis--the unidirectional TreT pathway--and discuss its physiological role as well as its phylogenetic distribution...
- CC1, a novel crenarchaeal DNA binding proteinXiao Luo
Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Fife KY16 9ST, UK
J Bacteriol 189:403-9. 2007..tenax. This protein binds single- and double-stranded DNAs with similar affinities. These properties are consistent with a role for CC1 as a crenarchaeal chromatin protein...
- Unusual pathways and enzymes of central carbohydrate metabolism in ArchaeaBettina Siebers
, Campus Essen, FB Biologie und Geografie, Mikrobiologie, , D-45117 Essen, Germany
Curr Opin Microbiol 8:695-705. 2005..Recent major advances in deciphering the complexity of archaeal central carbohydrate metabolism were gained by combination of classical biochemical and genomic-based approaches...
- Mechanism of the Schiff base forming fructose-1,6-bisphosphate aldolase: structural analysis of reaction intermediatesEsben Lorentzen
European Molecular Biology Laboratory, Hamburg Outstation, Germany
Biochemistry 44:4222-9. 2005..The structure thus allows for the first time the catalytic mechanism of ring opening to be unraveled...
- Crystal structure of an archaeal class I aldolase and the evolution of (betaalpha)8 barrel proteinsEsben Lorentzen
European Molecular Biology Laboratory, Hamburg Outstation, Notkestrasse 85, D 22603 Hamburg, Germany
J Biol Chem 278:47253-60. 2003..Structural comparisons, furthermore, establish an evolutionary link to the triosephosphate isomerases, a superfamily hitherto considered independent from the superfamily of aldolases...
- The hexokinase of the hyperthermophile Thermoproteus tenax. ATP-dependent hexokinases and ADP-dependent glucokinases, teo alternatives for glucose phosphorylation in ArchaeaChristine Dörr
Department of Microbiology, Universitat Duisburg Essen, Essen 45117, Germany
J Biol Chem 278:18744-53. 2003..The distribution of the two types of sugar kinases, differing in their cosubstrate as well as substrate specificity, within Archaea is discussed on the basis of physiological constraints of the respective organisms...
- The central carbohydrate metabolism of the hyperthermophilic crenarchaeote Thermoproteus tenax: pathways and insights into their regulationMelanie Zaparty
Department of Chemistry, Biofilm Centre, Molecular Enzyme Technology and Biochemistry, University of Duisburg Essen, Lotharstrasse, 47057 Duisburg, Germany
Arch Microbiol 190:231-45. 2008..The CCM pathways in T. tenax and the current understanding of their regulation are presented...
- Phosphoenolpyruvate synthetase and pyruvate, phosphate dikinase of Thermoproteus tenax: key pieces in the puzzle of archaeal carbohydrate metabolismBritta Tjaden
Department of Microbiology, Universitat Duisburg Essen, 45117 Essen, Germany
Mol Microbiol 60:287-98. 2006..Comparative genomic analyses indicate the coexistence of PEPS, PPDK and PK in other Archaea as well, suggesting a similar regulation of the carbohydrate metabolism in these organisms...
- The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of Sulfolobus solfataricus: a key-enzyme of the semi-phosphorylative branch of the Entner-Doudoroff pathwayThijs J G Ettema
Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, Norbyvagen 18C, 752 36 Uppsala, Sweden
Extremophiles 12:75-88. 2008..This work suggests an important role of GAPN in the regulation of carbon degradation via modifications of the EMP and the branched ED pathway in hyperthermophilic Archaea...
- The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluationHatim Ahmed
Department of Microbiology, University Duisburg Essen, Campus Essen, Universitätsstr 5, 45117 Essen, Germany
Biochem J 390:529-40. 2005..tenax and S. solfataricus. The existence of this branched ED pathway is yet another example of the versatility and flexibility of the central carbohydrate metabolic pathways in the archaeal domain...