Mehdi Shakibaei

Summary

Affiliation: University of Munich
Country: Germany

Publications

  1. ncbi request reprint Diverse roles of integrin receptors in articular cartilage
    M Shakibaei
    Institute of Anatomy, Musculoskeletal Research Group, Ludwig Maximilian University Munich, Munich, Germany
    Adv Anat Embryol Cell Biol 197:1-60. 2008
  2. doi request reprint Mesenchymal stem cells as a potential pool for cartilage tissue engineering
    C Csaki
    Ludwig Maximilians University Munich, Faculty of Medicine, Institute of Anatomy, Musculoskeletal Research Group, Pettenkoferstrasse 11, D 80336 Munich, Germany
    Ann Anat 190:395-412. 2008
  3. pmc Curcumin chemosensitizes 5-fluorouracil resistant MMR-deficient human colon cancer cells in high density cultures
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilian University Munich, Germany
    PLoS ONE 9:e85397. 2014
  4. pmc IGF-1 and PDGF-bb suppress IL-1β-induced cartilage degradation through down-regulation of NF-κB signaling: involvement of Src/PI-3K/AKT pathway
    Azadeh Montaseri
    Department of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
    PLoS ONE 6:e28663. 2011
  5. pmc Curcumin enhances the effect of chemotherapy against colorectal cancer cells by inhibition of NF-κB and Src protein kinase signaling pathways
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    PLoS ONE 8:e57218. 2013
  6. pmc Resveratrol mediated modulation of Sirt-1/Runx2 promotes osteogenic differentiation of mesenchymal stem cells: potential role of Runx2 deacetylation
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    PLoS ONE 7:e35712. 2012
  7. ncbi request reprint Resveratrol inhibits IL-1 beta-induced stimulation of caspase-3 and cleavage of PARP in human articular chondrocytes in vitro
    Mehdi Shakibaei
    Institute of Anatomy, Musculoskeletal Research Group, Ludwig Maximilian University Munich, Pettenkoferstrasse 11, D 80336 Munich
    Ann N Y Acad Sci 1095:554-63. 2007
  8. ncbi request reprint Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, D 80336 Munich, Germany
    Biochem Pharmacol 73:1434-45. 2007
  9. ncbi request reprint Curcumin protects human chondrocytes from IL-l1beta-induced inhibition of collagen type II and beta1-integrin expression and activation of caspase-3: an immunomorphological study
    Mehdi Shakibaei
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, 80336 Munich, Germany
    Ann Anat 187:487-97. 2005
  10. doi request reprint [Interaction between human chondrocytes and extracellular matrix in vitro: a contribution to autologous chondrocyte transplantation]
    M Shakibaei
    Forschungsgruppe Muskuloskelettales System, Institut fur Anatomie, Medizinische Fakultat, Ludwig Maximilians Universitat Munchen, Pettenkoferstrasse 11, 80336 Munchen
    Orthopade 37:440-7. 2008

Collaborators

Detail Information

Publications70

  1. ncbi request reprint Diverse roles of integrin receptors in articular cartilage
    M Shakibaei
    Institute of Anatomy, Musculoskeletal Research Group, Ludwig Maximilian University Munich, Munich, Germany
    Adv Anat Embryol Cell Biol 197:1-60. 2008
    ..This review focuses on the expression and function of beta1-integrins in articular chondrocytes, its role in the unique biology of these cells and its distribution in cartilage...
  2. doi request reprint Mesenchymal stem cells as a potential pool for cartilage tissue engineering
    C Csaki
    Ludwig Maximilians University Munich, Faculty of Medicine, Institute of Anatomy, Musculoskeletal Research Group, Pettenkoferstrasse 11, D 80336 Munich, Germany
    Ann Anat 190:395-412. 2008
    ....
  3. pmc Curcumin chemosensitizes 5-fluorouracil resistant MMR-deficient human colon cancer cells in high density cultures
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilian University Munich, Germany
    PLoS ONE 9:e85397. 2014
    ..Therefore, we investigated the effectiveness of 5-FU and plant polyphenol (curcumin) in context of DNA mismatch repair (MMR) status and CSC activity in 3D cultures of CRC cells...
  4. pmc IGF-1 and PDGF-bb suppress IL-1β-induced cartilage degradation through down-regulation of NF-κB signaling: involvement of Src/PI-3K/AKT pathway
    Azadeh Montaseri
    Department of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
    PLoS ONE 6:e28663. 2011
    ....
  5. pmc Curcumin enhances the effect of chemotherapy against colorectal cancer cells by inhibition of NF-κB and Src protein kinase signaling pathways
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    PLoS ONE 8:e57218. 2013
    ..Herein, we examined the effectiveness of a combination treatment regimen of 5-fluorouracil (5-FU) and curcumin in colorectal cancer (CRC) cells...
  6. pmc Resveratrol mediated modulation of Sirt-1/Runx2 promotes osteogenic differentiation of mesenchymal stem cells: potential role of Runx2 deacetylation
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    PLoS ONE 7:e35712. 2012
    ..This study determined whether activation of Sirt-1 (a NAD(+)-dependent histone deacetylase) by the phytoestrogen resveratrol affects osteogenic differentiation...
  7. ncbi request reprint Resveratrol inhibits IL-1 beta-induced stimulation of caspase-3 and cleavage of PARP in human articular chondrocytes in vitro
    Mehdi Shakibaei
    Institute of Anatomy, Musculoskeletal Research Group, Ludwig Maximilian University Munich, Pettenkoferstrasse 11, D 80336 Munich
    Ann N Y Acad Sci 1095:554-63. 2007
    ..These findings suggest that this dietary polyphenolic compound may have future applications in the nutraceutical-based therapy of human and animal OA...
  8. ncbi request reprint Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, D 80336 Munich, Germany
    Biochem Pharmacol 73:1434-45. 2007
    ..These results indicate that curcumin has nutritional potential as a naturally occurring anti-inflammatory agent for treating OA through suppression of NF-kappaB mediated IL-1beta/TNF-alpha catabolic signalling pathways in chondrocytes...
  9. ncbi request reprint Curcumin protects human chondrocytes from IL-l1beta-induced inhibition of collagen type II and beta1-integrin expression and activation of caspase-3: an immunomorphological study
    Mehdi Shakibaei
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, 80336 Munich, Germany
    Ann Anat 187:487-97. 2005
    ..Therefore curcumin may have novel therapeutic potential as an adjunct nutraceutical chondroprotective agent for treating OA and related osteoarticular disorders...
  10. doi request reprint [Interaction between human chondrocytes and extracellular matrix in vitro: a contribution to autologous chondrocyte transplantation]
    M Shakibaei
    Forschungsgruppe Muskuloskelettales System, Institut fur Anatomie, Medizinische Fakultat, Ludwig Maximilians Universitat Munchen, Pettenkoferstrasse 11, 80336 Munchen
    Orthopade 37:440-7. 2008
    ..A limiting factor for ACT is, however, the in vitro cultivation of chondrocytes because it leads to dedifferentiation. Therefore, the goal of this work was to optimize the monolayer culture of chondrocytes in vitro...
  11. doi request reprint Resveratrol suppresses interleukin-1beta-induced inflammatory signaling and apoptosis in human articular chondrocytes: potential for use as a novel nutraceutical for the treatment of osteoarthritis
    Mehdi Shakibaei
    Ludwig Maximilians University Munich, Faculty of Medicine, Institute of Anatomy, Musculoskeletal Research Group, Pettenkoferstrasse 11, D 80336 Munich, Germany
    Biochem Pharmacol 76:1426-39. 2008
    ..We propose that resveratrol should be explored further for the prophylactic treatment of osteoarthritis in humans and companion animals...
  12. pmc Resveratrol-mediated SIRT-1 interactions with p300 modulate receptor activator of NF-kappaB ligand (RANKL) activation of NF-kappaB signaling and inhibit osteoclastogenesis in bone-derived cells
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    J Biol Chem 286:11492-505. 2011
    ....
  13. pmc Curcumin synergizes with resveratrol to stimulate the MAPK signaling pathway in human articular chondrocytes in vitro
    Mehdi Shakibaei
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, 80336, Munich, Germany
    Genes Nutr 6:171-9. 2011
    ..These results suggest that combining these two natural compounds activates MEK/Erk signaling, a pathway that is involved in the maintenance of chondrocyte differentiation and survival...
  14. ncbi request reprint Anti-inflammatory and anti-catabolic effects of TENDOACTIVE® on human tenocytes in vitro
    M Shakibaei
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    Histol Histopathol 26:1173-85. 2011
    ..These results indicate that Tendoactive® has nutraceutical potential as an anti-inflammatory agent for treating tendinopathy through suppression of NF-κB mediated IL-1β catabolic signalling pathways in tenocytes...
  15. ncbi request reprint Igf-I extends the chondrogenic potential of human articular chondrocytes in vitro: molecular association between Sox9 and Erk1/2
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilians University, Pettenkoferstrasse 11, 80336 Munich, Germany
    Biochem Pharmacol 72:1382-95. 2006
    ..Treatment of chondrocyte cultures with Igf-I stabilizes chondrogenic potential, stimulates Sox9 and promotes molecular interactions between Erk and Sox9. These effects appear to be regulated by the integrin/MAPK signaling pathways...
  16. pmc Sirt-1 is required for the inhibition of apoptosis and inflammatory responses in human tenocytes
    Franziska Büsch
    Institute of Anatomy, Ludwig Maximilians University Munich, Munich 80336 Germany
    J Biol Chem 287:25770-81. 2012
    ..Overall, these results suggest for the first time that Sirt-1 can regulate p53 and NF-κB signaling via deacetylation, demonstrating a novel role for resveratrol in the treatment of tendinitis/tendinopathy...
  17. ncbi request reprint Effects of curcumin (diferuloylmethane) on nuclear factor kappaB signaling in interleukin-1beta-stimulated chondrocytes
    Gundula Schulze-Tanzil
    Charité University Medical School Berlin, Institute of Anatomy, Department of Cell and Neurobiology, Campus Benjamin Franklin, Königin Luise Strasse 15, D 14195 Berlin, Germany
    Ann N Y Acad Sci 1030:578-86. 2004
    ..Therefore, curcumin antagonizes crucial catabolic effects of IL-1beta signaling that are known to contribute to the pathogenesis of osteoarthritis...
  18. ncbi request reprint Loss of chondrogenic potential in dedifferentiated chondrocytes correlates with deficient Shc-Erk interaction and apoptosis
    Gundula Schulze-Tanzil
    Charite University Medical School, Campus Benjamin Franklin, Institute of Anatomy, Department of Cell and Neurobiology, Berlin, Germany
    Osteoarthritis Cartilage 12:448-58. 2004
    ..We also characterized the molecular traits of irreversibly dedifferentiated cells...
  19. pmc Resveratrol modulates interleukin-1β-induced phosphatidylinositol 3-kinase and nuclear factor κB signaling pathways in human tenocytes
    Franziska Büsch
    Institute of Anatomy, Ludwig Maximilian University Munich, 80336 Munich, Germany
    J Biol Chem 287:38050-63. 2012
    ..Overall, these results demonstrate that activated Sirt-1 plays an essential role in the anti-inflammatory effects of resveratrol and this may be mediated at least in part through inhibition/deacetylation of PI3K and NF-κB...
  20. doi request reprint Three-dimensional high-density co-culture with primary tenocytes induces tenogenic differentiation in mesenchymal stem cells
    Philipp R A Schneider
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilian University of Munich, Pettenkoferstrasse 11, 80336 Munich, Germany
    J Orthop Res 29:1351-60. 2011
    ..This approach may prove to be of practical benefit in future tissue engineering and tendon regenerative medicine research...
  21. pmc Curcumin modulates nuclear factor kappaB (NF-kappaB)-mediated inflammation in human tenocytes in vitro: role of the phosphatidylinositol 3-kinase/Akt pathway
    Constanze Buhrmann
    Institute of Anatomy, Ludwig Maximilian University Munich, 80336 Munich, Germany
    J Biol Chem 286:28556-66. 2011
    ....
  22. pmc Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1beta-induced NF-kappaB-mediated inflammation and apoptosis
    Constanze Csaki
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, 80336 Munich, Germany
    Arthritis Res Ther 11:R165. 2009
    ..Naturally occurring polyphenolic compounds, such as curcumin and resveratrol, are potent agents for modulating inflammation. Both compounds mediate their effects by targeting the NF-kappaB signalling pathway...
  23. pmc Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment
    Constanze Buhrmann
    Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, D 80336 Munich, Germany
    Arthritis Res Ther 12:R127. 2010
    ..Therefore, the aim of the present study was to evaluate the influence of curcumin on IL-1β-induced NF-κB signalling pathway in MSCs during chondrogenic differentiation...
  24. ncbi request reprint Anabolic actions of IGF-I and TGF-beta1 on Interleukin-1beta-treated human articular chondrocytes: evaluation in two and three dimensional cultures
    Claudia Seifarth
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    Histol Histopathol 24:1245-62. 2009
    ..The additive action of these growth factors on chondrocytes may find practical applications in the fields of OA and cartilage tissue engineering...
  25. ncbi request reprint [Effect of a Harpagophytum procumbens DC extract on matrix metalloproteinases in human chondrocytes in vitro]
    Gundula Schulze-Tanzil
    Institut fur Anatomie, Universitatsklinikum Benjamin Franklin, Charite Universitatsmedizin Berlin, Berlin, Germany
    Arzneimittelforschung 54:213-20. 2004
    ..In these in vitro experiments the JF-extract showed a higher efficacy than the JM-extract...
  26. doi request reprint Resveratrol addiction: to die or not to die
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    Mol Nutr Food Res 53:115-28. 2009
    ..These attributes of resveratrol are discussed in detail in this review...
  27. ncbi request reprint Regulation of inflammation signalling by resveratrol in human chondrocytes in vitro
    Constanze Csaki
    Ludwig Maximilians University Munich, Faculty of Medicine, Institute of Anatomy, Pettenkoferstrasse 11, Munich, Germany
    Biochem Pharmacol 75:677-87. 2008
    ..Further studies should be undertaken to define a possible implication of resveratrol in osteoarthritis therapy and cartilage tissue engineering...
  28. pmc TNF-alpha-induced mitochondrial alterations in human T cells requires FADD and caspase-8 activation but not RIP and caspase-3 activation
    Mehdi Shakibaei
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilian University Munich, Munich, Germany
    Antioxid Redox Signal 13:821-31. 2010
    ....
  29. ncbi request reprint Tissue engineering of autologous human heart valves using cryopreserved vascular umbilical cord cells
    Ralf Sodian
    Department of Cardiothoracic and Vascular Surgery, Deutsches Herzzentrum Berlin, Germany
    Ann Thorac Surg 81:2207-16. 2006
    ....
  30. doi request reprint Use of human umbilical cord blood-derived progenitor cells for tissue-engineered heart valves
    Ralf Sodian
    Department of Cardiovascular Surgery, Ludwig Maximilians University, Munich, Germany
    Ann Thorac Surg 89:819-28. 2010
    ..Here we describe the use of cryopreserved umbilical cord blood-derived CD133(+) cells as a single cell source for the tissue engineering of heart valves...
  31. pmc Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1β-Induced NF-κB Activation in Canine Articular Chondrocytes
    Mehdi Shakibaei
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilian University Munich, 80336 Munich, Germany
    Evid Based Complement Alternat Med 2012:509383. 2012
    ....
  32. ncbi request reprint Redox regulation of apoptosis by members of the TNF superfamily
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilians University Munich, Munich, Germany
    Antioxid Redox Signal 7:482-96. 2005
    ..Here we review the evidence linking reactive oxygen intermediates to cytokine-induced cell death mediated by TNF-alpha/beta, Fas, TRAIL, TNF-like weak inducer of apoptosis (TWEAK), and vascular endothelial cell growth inhibitor (VEGI)...
  33. ncbi request reprint Redifferentiation of dedifferentiated human chondrocytes in high-density cultures
    G Schulze-Tanzil
    Institute of Anatomy, Freie Universitat Berlin, Königin Luise Strasse 15, 14195 Berlin, Germany
    Cell Tissue Res 308:371-9. 2002
    ..Such high-density cultures might serve as a model system to initiate and promote the redifferentiation of chondrocytes and to provide sufficient quantities of differentiated chondrocytes for autologous chondrocyte transplantation...
  34. ncbi request reprint Inhibition of chondrogenesis by integrin antibody in vitro
    M Shakibaei
    Institute of Anatomy, Free University of Berlin, Germany
    Exp Cell Res 240:95-106. 1998
    ..These findings indicate that beta 1-integrins play a crucial role in early cartilage differentiation and point to a possible important cell-matrix interaction in the induction of chondrogenesis...
  35. ncbi request reprint Beta1-integrins in the cartilage matrix
    M Shakibaei
    Institut fur Anatomie, Freie Universitat Berlin, Königin Luise Strasse 15, D 14195 Berlin, Germany
    Cell Tissue Res 296:565-73. 1999
    ..The question as to whether they have a function in the cartilage matrix, such as interlinking, in the matrix organization or in the stabilization of matrix components remains to be elucidated...
  36. doi request reprint Midkine acts as proangiogenic cytokine in hypoxia-induced angiogenesis
    Ludwig T Weckbach
    Walter Brendel Centre of Experimental Medicine, Ludwig Maximilians University, Munich, Germany
    Am J Physiol Heart Circ Physiol 303:H429-38. 2012
    ..01). These unanticipated results identified endothelial cells as the source of soluble MK in the vascular system during hypoxia and defined MK as a pivotal player of angiogenesis during ischemia in nonmalignant tissue...
  37. ncbi request reprint Changes in integrin expression during chondrogenesis in vitro: an immunomorphological study
    M Shakibaei
    Institute of Anatomy, Free University of Berlin, Germany
    J Histochem Cytochem 43:1061-9. 1995
    ..Therefore, a strict correlation between the collagen type synthesized by the cells and the appropriate receptor presented by the cells is suggested...
  38. ncbi request reprint Expression of the VEGF receptor-3 in osteoarthritic chondrocytes: stimulation by interleukin-1 beta and association with beta 1-integrins
    M Shakibaei
    Institute of Anatomy, Freie Universitat Berlin, Königin Luise Strasse 15, 14195 Berlin, Germany
    Histochem Cell Biol 120:235-41. 2003
    ..Further work needs to be done to elucidate downstream signaling events activated by these receptors...
  39. doi request reprint Co-culture of canine mesenchymal stem cells with primary bone-derived osteoblasts promotes osteogenic differentiation
    C Csaki
    Musculoskeletal Research Group, Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, 80336, Munich, Germany
    Histochem Cell Biol 131:251-66. 2009
    ..This approach may prove to be of practical benefit in future tissue engineering and regenerative medicine research...
  40. ncbi request reprint Co-localization of integrins and matrix metalloproteinases in the extracellular matrix of chondrocyte cultures
    G Schulze-Tanzil
    Institute of Anatomy, , Germany
    Histol Histopathol 16:1081-9. 2001
    ..Further investigations are needed to elucidate the functional association between beta1-integrins and MMPs in chondrocytes...
  41. ncbi request reprint Beta1-integrins co-localize with Na, K-ATPase, epithelial sodium channels (ENaC) and voltage activated calcium channels (VACC) in mechanoreceptor complexes of mouse limb-bud chondrocytes
    M Shakibaei
    Institut fur Anatomie, Freie Universitat Berlin, Berlin, Germany
    Histol Histopathol 18:343-51. 2003
    ....
  42. ncbi request reprint Expression of the urokinase-type plasminogen activator receptor in human articular chondrocytes: association with caveolin and beta 1-integrin
    W Schwab
    Institute of Anatomy, Technical University Dresden, Medical Faculty Carl Gustav Carus, Fiedlerstrasse 42, 01307 Dresden, Germany
    Histochem Cell Biol 115:317-23. 2001
    ..The localization of uPAR in caveolae and its close association with caveolin and beta 1-integrin points to a significance of uPAR-mediated signaling pathways in human chondrocytes...
  43. ncbi request reprint Interleukin-1beta-induced expression of the urokinase-type plasminogen activator receptor and its co-localization with MMPs in human articular chondrocytes
    W Schwab
    Institute of Anatomy, Technical University Dresden, Germany
    Histol Histopathol 19:105-12. 2004
    ..Interfering with uPAR signaling may present a novel target in arthritis therapy to prevent excessive proteolytic degradation...
  44. ncbi request reprint Characterization of caveolins from human knee joint catilage: expression of caveolin-1, -2, and -3 in chondrocytes and association with integrin beta1
    W Schwab
    Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Germany
    Histochem Cell Biol 113:221-5. 2000
    ..The data stimulate further investigations on the role of the caveolin-integrin complex for integrin-mediated signaling pathways in chondrocytes...
  45. ncbi request reprint Effects of the antirheumatic remedy hox alpha--a new stinging nettle leaf extract--on matrix metalloproteinases in human chondrocytes in vitro
    G Schulze-Tanzil
    Institute of Anatomy, Freie Universitat Berlin, Germany
    Histol Histopathol 17:477-85. 2002
    ..These results suggest that the monosubstance 13-HOTrE is one of the more active antiinflammatory substances in Hox alpha and that Hox alpha may be a promising remedy for therapy of inflammatory joint diseases...
  46. ncbi request reprint Ultrastructural characterization of murine limb buds after in vitro exposure to grepafloxacin and other fluoroquinolones
    Mehdi Shakibaei
    Institute of Anatomy, Benjamin Franklin Medical Center, Freie Universitat Berlin, Germany
    Arch Toxicol 75:725-33. 2002
    ..Grepafloxacin showed a relatively high affinity for Ca2+ in the fluorescence assay, which was more pronounced than the affinities of six other fluoroquinolones tested before...
  47. ncbi request reprint Ultrastructural changes induced by the des-F(6)-quinolone garenoxacin (BMS-284756) and two fluoroquinolones in Achilles tendon from immature rats
    Mehdi Shakibaei
    Institute of Anatomy, Benjamin Franklin Medical Center, Freie Universitat Berlin, 14195 Berlin, Germany
    Arch Toxicol 77:521-6. 2003
    ....
  48. ncbi request reprint Molecular anatomy of the perivascular sheath in human placental stem villi: the contractile apparatus and its association to the extracellular matrix
    R Graf
    Institut fur Anatomie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Königin Luise Str 15, D 14195 Berlin, Germany
    Cell Tissue Res 290:601-7. 1997
    ....
  49. ncbi request reprint Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study
    C Csaki
    Institute of Anatomy, Musculoskeletal Research Group, Ludwig Maximilian University Munich, Pettenkoferstrasse 11, 80336, Munich, Germany
    Histochem Cell Biol 128:507-20. 2007
    ..These results demonstrate, at the biochemical, morphological and ultrastructural levels, the multipotency of cMSCs and thus highlight their potential therapeutic value for cell-based tissue engineering...
  50. ncbi request reprint Cultivation of human tenocytes in high-density culture
    G Schulze-Tanzil
    Campus Benjamin Franklin, Institute of Anatomy, Department of Cell and Neurobiology, Charite Medicine University Berlin, Königin Luise Strasse 15, 14195, Germany
    Histochem Cell Biol 122:219-28. 2004
    ....
  51. ncbi request reprint Inhibition of mitogen-activated protein kinase kinase induces apoptosis of human chondrocytes
    M Shakibaei
    Institute of Anatomy, Freie Universitat Berlin, Königin Luise Strasse 15, D 14195 Berlin, Germany
    J Biol Chem 276:13289-94. 2001
    ..These data stimulate further investigations on the role of mitogen-activated protein kinase pathways in human chondrocytes...
  52. pmc Signal transduction by beta1 integrin receptors in human chondrocytes in vitro: collaboration with the insulin-like growth factor-I receptor
    M Shakibaei
    Institute of Anatomy, Free University Berlin, Königin Luise Strasse 15, D 14195 Berlin, Germany
    Biochem J 342:615-23. 1999
    ..These events may play a role during chondrocyte differentiation...
  53. ncbi request reprint Apoptosis and the loss of chondrocyte survival signals contribute to articular cartilage degradation in osteoarthritis
    Robert Goggs
    Connective Tissue Research Group, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 7ZJ, UK
    Vet J 166:140-58. 2003
    ..Understanding of the interactions that promote chondrocyte apoptosis and cartilage hypocellularity is essential for developing appropriately targeted therapies for inhibition of chondrocyte apoptosis and the treatment of OA...
  54. ncbi request reprint Increase of fibronectin and osteopontin in porcine hearts following ischemia and reperfusion
    Peter Kossmehl
    Institute of Clinical Pharmacology and Toxicology, Benjamin Franklin Medical Center, Charite University Medical School, Garystr 5, 14195 Berlin, Germany
    J Mol Med (Berl) 83:626-37. 2005
    ..Moreover, OPN, fibronectin and TGF-beta1 protein and gene expression are elevated after ischemia and reperfusion in the ex vivo working hemoperfused porcine heart model...
  55. ncbi request reprint Weightlessness induced apoptosis in normal thyroid cells and papillary thyroid carcinoma cells via extrinsic and intrinsic pathways
    Peter Kossmehl
    Institute of Clinical Pharmacology and Toxicology, Benjamin Franklin Medical Center, Freie Universitat Berlin, 14195 Berlin, Germany
    Endocrinology 144:4172-9. 2003
    ..Gravitational unloading affects the mitochondria and thereby may trigger apoptosis in thyroid cells subjected to weightlessness by clinorotation...
  56. ncbi request reprint Short-term culture of human neonatal myofibroblasts seeded using a novel three-dimensional rotary seeding device
    Cora Lueders
    Department of Thoracic and Cardiovascular Surgery, Laboratory for Tissue Engineering, Berlin, Germany
    ASAIO J 52:310-4. 2006
    ..Our newly developed cell-seeding device enables sterile conditions and optimal cell distribution for the controlled fabrication of autologous tissue-engineered heart valve constructs...
  57. ncbi request reprint Intraluminal application of vascular endothelial growth factor enhances healing of microvascular anastomosis in a rat model
    Manfred Infanger
    Department of Trauma and Reconstructive Surgery, Charite University Medicine Berlin, Benjamin Franklin Campus, Hindenburgdamm 30, DE 12200 Berlin, Germany
    J Vasc Res 42:202-13. 2005
    ..We investigated the effect of vascular endothelial growth factor (VEGF) and its impact on the vascular remodeling process and reendothelialization after microsurgery...
  58. ncbi request reprint Effects of 2,3,7,8-tetrachloro-dibenzo-p-dioxin on the extracellular matrix of the thymus in juvenile marmosets (Callithrix jacchus)
    Christian Nottebrock
    Institute of Clinical Pharmacology and Toxicology, Charite Campus Benjamin Franklin, Garystrasse 5, 14195 Berlin, Germany
    Toxicology 226:197-207. 2006
    ..The extracellular matrix may play a more central role in mediating adverse effects of TCDD in organs than yet acknowledged...
  59. ncbi request reprint Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells
    Daniela Grimm
    Institute of Clinical Pharmacology and Toxicology, Benjamin Franklin Medical Center, Freie Universitat Berlin, Germany
    FASEB J 16:604-6. 2002
    ....
  60. ncbi request reprint Early onset of chondroitin sulfate and osteopontin expression in angiotensin II-dependent left ventricular hypertrophy
    Lars Rothermund
    Institut fur Klinische Pharmakologie und Toxikologie, Berlin, Germany
    Am J Hypertens 15:644-52. 2002
    ..The objective of this study was to analyze extracellular matrix protein (ECMP) expression in Ang II-dependent left ventricular (LV) hypertrophy (LVH), LV dysfunction, and to investigate right ventricular changes...
  61. ncbi request reprint Nutraceutical therapies for degenerative joint diseases: a critical review
    Robert Goggs
    Connective Tissue Research Group, Faculty of Veterinary Science, University of Liverpool, Liverpool, United Kingdom
    Crit Rev Food Sci Nutr 45:145-64. 2005
    ....
  62. ncbi request reprint A hypothesis for the origin and pathogenesis of rheumatoid diseases
    Wolfgang Lorenz
    Institute of Indoor Diagnostics, Marconistrasse 23, 40589 Duesseldorf, Germany
    Rheumatol Int 26:641-54. 2006
    ..The present study leads to the hypothesis that LPS may bind to procollagen, as they bind to scavenger receptors. This procollagen endotoxin complex may block tropocollagen synthesis...
  63. ncbi request reprint Fluoroquinolones cause changes in extracellular matrix, signalling proteins, metalloproteinases and caspase-3 in cultured human tendon cells
    Judith Sendzik
    Institute of Clinical Pharmacology and Toxicology, Department of Toxicology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Garystr 5, 14195 Berlin, Germany
    Toxicology 212:24-36. 2005
    ..Our results provide evidence that besides changes in receptor and signalling proteins apoptosis has to be considered as a final event in the pathogenesis of fluoroquinolone-induced tendopathies...
  64. ncbi request reprint Accelerated mitochondrial adenosine diphosphate/adenosine triphosphate transport improves hypertension-induced heart disease
    Thomas Walther
    Charite Universitatsmedizin, Campus Benjamin Franklin, Department of Cardiology and Pneumonology, Hindenburgdamm 30, 12200 Berlin, Germany
    Circulation 115:333-44. 2007
    ..An adequate exchange of ATP and ADP over the inner mitochondrial membrane by the adenine nucleotide translocase (ANT) is thereby essential to guarantee the cellular energy supply...
  65. ncbi request reprint Regulation of 2-deoxy-D-glucose transport, lactate metabolism, and MMP-2 secretion by the hypoxia mimetic cobalt chloride in articular chondrocytes
    Ali Mobasheri
    Molecular Pathogenesis and Connective Tissue Research, Department of Veterinary Preclinical Sciences, Faculty of Veterinary Science, University of Liverpool, Brownlow Hill and Crown Street, Liverpool, UK
    Ann N Y Acad Sci 1091:83-93. 2006
    ..Upregulation of MMP-2 and the build-up of lactic acid will have detrimental effects on the extracellular matrix and may contribute to the pathogenesis and progression of osteoarthritis (OA)...
  66. ncbi request reprint Simulated weightlessness changes the cytoskeleton and extracellular matrix proteins in papillary thyroid carcinoma cells
    Manfred Infanger
    Department of Trauma and Reconstructive Surgery, Charite University Medical School, Center of Space Medicine, Benjamin Franklin Medical Center, 12200, Berlin, Germany
    Cell Tissue Res 324:267-77. 2006
    ..The amount of E-cadherin was enhanced time-dependently. We suggest that simulated weightlessness rapidly affects the cytoskeleton of papillary thyroid carcinoma cells and increases the amount of ECM proteins in a time-dependent manner...
  67. ncbi request reprint Subcellular pathways of beta-endorphin synthesis, processing, and release from immunocytes in inflammatory pain
    Shaaban A Mousa
    Klinik fur Anaesthesiologie und operative Intensivmedizin, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, D 12200 Berlin, Germany
    Endocrinology 145:1331-41. 2004
    ..Taken together, our results indicate that immune cells express the entire machinery required for POMC processing into functionally active peptides such as END and are able to release these peptides from secretory granules...
  68. ncbi request reprint Electron microscopic findings in BAL of a fire-eater after petroleum aspiration
    Olaf Burkhardt
    Department of Chest and Infectious Diseases, Hospital Heckeshorn, Berlin, Germany
    Chest 124:398-400. 2003
    ..Despite a large and necrotizing infiltration of the right lower lobe, the clinical course was uneventful with complete recovery...
  69. pmc Effects of the Des-F(6)-quinolone garenoxacin (BMS-284756), in comparison to those of ciprofloxacin and ofloxacin, on joint cartilage in immature rats
    Eva Maria Kappel
    Institute of Clinical Pharmacology and Toxicology, Benjamin Franklin Medical Center, Freie Universitat Berlin, Germany
    Antimicrob Agents Chemother 46:3320-2. 2002
    ..5 mg/liter after administration of a dose of 600 mg/kg once daily for 5 days. Assuming that this model is predictive of human risk, BMS-284756 and ciprofloxacin should be more suitable for pediatric use than ofloxacin...
  70. ncbi request reprint Longterm conditions of mimicked weightlessness influences the cytoskeleton in thyroid cells
    Manfred Infanger
    Department of Trauma and Reconstructive Surgery, Charite University Medicine, Berlin, Germany
    J Gravit Physiol 11:P169-72. 2004
    ..Future studies to clarify the pathway that regulate the observed integrin activation and the mechanisms by which they function have to be performed...