Edzard Schwedhelm

Summary

Affiliation: University of Hamburg
Country: Germany

Publications

  1. pmc Plasma symmetric dimethylarginine reference limits from the Framingham offspring cohort
    Edzard Schwedhelm
    Institute of Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Clin Chem Lab Med 49:1907-10. 2011
  2. pmc A study of endothelial function and circulating asymmetric dimethylarginine levels in people with Type 1 diabetes without macrovascular disease or microalbuminuria
    Latika Sibal
    Newcastle University, Newcastle upon Tyne, UK
    Cardiovasc Diabetol 8:27. 2009
  3. pmc Pharmacokinetic and pharmacodynamic properties of oral L-citrulline and L-arginine: impact on nitric oxide metabolism
    Edzard Schwedhelm
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Centre Hamburg Eppendorf, Germany
    Br J Clin Pharmacol 65:51-9. 2008
  4. ncbi request reprint High-throughput liquid chromatographic-tandem mass spectrometric determination of arginine and dimethylated arginine derivatives in human and mouse plasma
    Edzard Schwedhelm
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 851:211-9. 2007
  5. ncbi request reprint Quantification of ADMA: analytical approaches
    Edzard Schwedhelm
    Institut fur Experimentelle und Klinische Pharmakologie, Universitatsklinikum Hamburg Eppendorf, Germany
    Vasc Med 10:S89-95. 2005
  6. ncbi request reprint Liquid chromatography-tandem mass spectrometry method for the analysis of asymmetric dimethylarginine in human plasma
    Edzard Schwedhelm
    Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Clin Chem 51:1268-71. 2005
  7. pmc Asymmetric dimethylarginine reference intervals determined with liquid chromatography-tandem mass spectrometry: results from the Framingham offspring cohort
    Edzard Schwedhelm
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Clin Chem 55:1539-45. 2009
  8. ncbi request reprint Urinary 8-iso-prostaglandin F2alpha as a risk marker in patients with coronary heart disease: a matched case-control study
    Edzard Schwedhelm
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Circulation 109:843-8. 2004
  9. ncbi request reprint Application of gas chromatography-mass spectrometry for analysis of isoprostanes: their role in cardiovascular disease
    Edzard Schwedhelm
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Clin Chem Lab Med 41:1552-61. 2003
  10. ncbi request reprint Telmisartan improves endothelial function in patients with essential hypertension
    Ralf A Benndorf
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Medical Center, Hamburg Eppendorf, Germany
    J Cardiovasc Pharmacol 50:367-71. 2007

Detail Information

Publications71

  1. pmc Plasma symmetric dimethylarginine reference limits from the Framingham offspring cohort
    Edzard Schwedhelm
    Institute of Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Clin Chem Lab Med 49:1907-10. 2011
    ..Furthermore, recent work implicates SDMA in the pathogenesis of cardiovascular disease. To date, reference limits for SDMA plasma concentrations in healthy individuals are lacking...
  2. pmc A study of endothelial function and circulating asymmetric dimethylarginine levels in people with Type 1 diabetes without macrovascular disease or microalbuminuria
    Latika Sibal
    Newcastle University, Newcastle upon Tyne, UK
    Cardiovasc Diabetol 8:27. 2009
    ..The aim of the present study was to measure circulating ADMA, and define its association with endothelial dysfunction and endothelial markers in people with Type 1 diabetes with low likelihood of macrovascular disease...
  3. pmc Pharmacokinetic and pharmacodynamic properties of oral L-citrulline and L-arginine: impact on nitric oxide metabolism
    Edzard Schwedhelm
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Centre Hamburg Eppendorf, Germany
    Br J Clin Pharmacol 65:51-9. 2008
    ..In contrast, L-citrulline is readily absorbed and at least in part converted to L-arginine. The aim of our study was to assess this metabolic conversion and its subsequent pharmacodynamic effects...
  4. ncbi request reprint High-throughput liquid chromatographic-tandem mass spectrometric determination of arginine and dimethylated arginine derivatives in human and mouse plasma
    Edzard Schwedhelm
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 851:211-9. 2007
    ..37+/-0.07 microM in healthy humans (n=85), respectively, and 44+/-14, 0.72+/-0.23, and 0.19+/-0.06 microM in C57BL/6 mice. Also, the molar ratios of arginine to ADMA were different in man and mice, i.e. 166+/-50 and 85+/-22, respectively...
  5. ncbi request reprint Quantification of ADMA: analytical approaches
    Edzard Schwedhelm
    Institut fur Experimentelle und Klinische Pharmakologie, Universitatsklinikum Hamburg Eppendorf, Germany
    Vasc Med 10:S89-95. 2005
    ..This GC-MS approach has been further improved by Albsmeier et al by introducing labelled ADMA as an internal standard. As an alternative to existing methods, a commercially available ELISA kit has recently been developed and validated...
  6. ncbi request reprint Liquid chromatography-tandem mass spectrometry method for the analysis of asymmetric dimethylarginine in human plasma
    Edzard Schwedhelm
    Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Clin Chem 51:1268-71. 2005
  7. pmc Asymmetric dimethylarginine reference intervals determined with liquid chromatography-tandem mass spectrometry: results from the Framingham offspring cohort
    Edzard Schwedhelm
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Clin Chem 55:1539-45. 2009
    ..Relatively small differences in ADMA concentrations between healthy individuals and those with disease underscore the need to formulate reference intervals that may aid risk stratification of individuals...
  8. ncbi request reprint Urinary 8-iso-prostaglandin F2alpha as a risk marker in patients with coronary heart disease: a matched case-control study
    Edzard Schwedhelm
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Circulation 109:843-8. 2004
    ..We tested the hypothesis that risk factors of CHD are associated with abundant systemic oxidative stress...
  9. ncbi request reprint Application of gas chromatography-mass spectrometry for analysis of isoprostanes: their role in cardiovascular disease
    Edzard Schwedhelm
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Clin Chem Lab Med 41:1552-61. 2003
    ..Results from several clinical studies are included indicating the validity of isoprostanes as surrogate parameters of oxidative stress in cardiovascular disease...
  10. ncbi request reprint Telmisartan improves endothelial function in patients with essential hypertension
    Ralf A Benndorf
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Medical Center, Hamburg Eppendorf, Germany
    J Cardiovasc Pharmacol 50:367-71. 2007
    ..We therefore investigated whether the new ARB telmisartan improves endothelial function and renal vascular resistance in patients with essential hypertension...
  11. pmc Reference intervals for plasma L-arginine and the L-arginine:asymmetric dimethylarginine ratio in the Framingham Offspring Cohort
    Nicole Lüneburg
    Institute of Clinical Pharmacology and Toxicology, University Medical Center, Hamburg Eppendorf, Germany
    J Nutr 141:2186-90. 2011
    ..We report reference levels for plasma L-arginine and for the L-arginine:ADMA ratio that may be helpful for evaluation of the effects of L-arginine supplementation in participants with an impaired L-arginine/NO pathway...
  12. ncbi request reprint Mobilization of putative high-proliferative-potential endothelial colony-forming cells during antihypertensive treatment in patients with essential hypertension
    Ralf A Benndorf
    Institute of Experimental and Clinical Pharmacology and Toxicology, Department of Clinical Pharmacology, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Stem Cells Dev 16:329-38. 2007
    ....
  13. ncbi request reprint Pharmacokinetics of oral doses of telmisartan and nisoldipine, given alone and in combination, in patients with essential hypertension
    Milica Bajcetic
    Institute of Experimental and Clinical Pharmacology and Toxicology, Clinical Pharmacology Unit, University Hospital Hamburg Eppendorf, Martinistrasse 521, D 20246 Hamburg
    J Clin Pharmacol 47:295-304. 2007
    ..Larger crossover trials will have to establish these observations and investigate whether interaction of both drugs affects telmisartan efficacy and tolerability in clinical use...
  14. ncbi request reprint Simultaneous assessment of endothelial function, nitric oxide synthase activity, nitric oxide-mediated signaling, and oxidative stress in individuals with and without hypercholesterolemia
    Renke Maas
    Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie, Universitatsklinikum Hamburg Eppendorf, Hamburg, Germany
    Clin Chem 54:292-300. 2008
    ..It was the aim of this study to directly estimate and compare whole-body NO production in normo- and hypercholesterolemia by applying a nonradioactive stable isotope dilution technique in vivo...
  15. pmc Dimethylarginine dimethylaminohydrolase1 is an organ-specific mediator of end organ damage in a murine model of hypertension
    Karsten Sydow
    Department of Interventional Cardiology, Hamburg University Heart Center, University Hospital Hamburg Eppendorf, Hamburg, Germany
    PLoS ONE 7:e48150. 2012
    ....
  16. ncbi request reprint Telmisartan improves insulin sensitivity in nondiabetic patients with essential hypertension
    Ralf A Benndorf
    Department of Clinical Pharmacology, Institute of Experimental and Clinical Pharmacology and Toxicology, University Hospital Hamburg Eppendorf, D 20246 Hamburg, Germany
    Metabolism 55:1159-64. 2006
    ..Future studies will demonstrate whether these telmisartan-induced effects may contribute to a blood pressure-independent reduction in cardiovascular morbidity...
  17. pmc Dimethylarginine dimethylaminohydrolase-1 transgenic mice are not protected from ischemic stroke
    Frank Leypoldt
    Department of Neurology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    PLoS ONE 4:e7337. 2009
    ..ADMA is a predictor of cardiovascular events and mortality in humans. It is eliminated primarily by enzymatic activity of dimethylarginine dimethylaminohydrolase (DDAH)...
  18. pmc Plasma asymmetric dimethylarginine and incidence of cardiovascular disease and death in the community
    Rainer H Boger
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Circulation 119:1592-600. 2009
    ..Although elevated ADMA has been associated with an increased risk of cardiovascular disease events and death in referral samples, the prognostic significance of ADMA in the community has not been adequately evaluated...
  19. ncbi request reprint Elevated plasma concentrations of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine predict adverse events in patients undergoing noncardiac surgery
    Renke Maas
    University Hospital Hamburg Eppendorf, Institute of Experimental and Clinical Pharmacology and Toxicology, Hamburg, Germany
    Crit Care Med 35:1876-81. 2007
    ..We aimed to investigate elevated plasma ADMA concentrations as a prospective risk marker for adverse events in patients undergoing noncardiac surgery...
  20. doi request reprint L-Arginine enhances the triglyceride-lowering effect of simvastatin in patients with elevated plasma triglycerides
    Friedrich Schulze
    Center for Experimental Medicine, Institute for Experimental and Clinical Pharmacology, University Medical Center Hamburg Eppendorf, Hamburg 20246, Germany
    Nutr Res 29:291-7. 2009
    ..We conclude from our data that L-arginine enhances the effects of simvastatin on lipid metabolism, but it has no triglyceride-lowering effects when given alone...
  21. doi request reprint Symmetric dimethylarginine predicts all-cause mortality following ischemic stroke
    Friedrich Schulze
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Atherosclerosis 208:518-23. 2010
    ..ADMA is a predictor of cardiovascular events and mortality in diverse populations...
  22. doi request reprint Extensive characterization of the human DDAH1 transgenic mice
    Edzard Schwedhelm
    Institute of Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Pharmacol Res 60:494-502. 2009
    ....
  23. ncbi request reprint Clopidogrel improves systemic endothelial nitric oxide bioavailability in patients with coronary artery disease: evidence for antioxidant and antiinflammatory effects
    Thomas Heitzer
    Universitäres Herzzentrum Hamburg, Universitatsklinikum Hamburg Eppendorf, Germany
    Arterioscler Thromb Vasc Biol 26:1648-52. 2006
    ....
  24. ncbi request reprint FK778 and tacrolimus prevent the development of obliterative airway disease after heterotopic rat tracheal transplantation
    Tobias Deuse
    Department of Cardiovascular Surgery, University Hospital Hamburg Eppendorf, Hamburg, Germany
    J Heart Lung Transplant 24:1844-54. 2005
    ..The effectiveness of the novel immunosuppressive agent FK778 and of tacrolimus to prevent the development of obliterative airway disease (OAD) was investigated in an animal model...
  25. doi request reprint Asymmetrical dimethylarginine is increased in plasma and decreased in cerebrospinal fluid of patients with Alzheimer's disease
    Sönke Arlt
    Department of Psychiatry and Psychotherapy, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Dement Geriatr Cogn Disord 26:58-64. 2008
    ..Concerning Alzheimer's disease (AD), there are reports on altered cerebral NO metabolism, but only few studies on ADMA concentrations in plasma and cerebrospinal fluid (CSF)...
  26. doi request reprint Nitroxyl exacerbates ischemic cerebral injury and oxidative neurotoxicity
    Chi Un Choe
    Department of Neurology, University Hospital Hamburg Eppendorf, Hamburg, Germany
    J Neurochem 110:1766-73. 2009
    ..Taken together, our data indicate that HNO might worsen cerebral ischemia-reperfusion injury by increasing oxidative stress and decreasing brain perfusion at concentrations shown to be cardioprotective in vivo...
  27. ncbi request reprint Hypercholesterolemia impairs basal nitric oxide synthase turnover rate: a study investigating the conversion of L-[guanidino-(15)N(2)]-arginine to (15)N-labeled nitrate by gas chromatography--mass spectrometry
    Rainer H Boger
    Institute of Clinical Pharmacology, Medical School, Hannover, Germany Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Nitric Oxide 11:1-8. 2004
    ....
  28. ncbi request reprint Acute effects of various fast-food meals on vascular function and cardiovascular disease risk markers: the Hamburg Burger Trial
    Tanja K Rudolph
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Am J Clin Nutr 86:334-40. 2007
    ..High-fat meals have negative effects on endothelial function, but vitamin-rich side orders may prevent these negative effects...
  29. ncbi request reprint Asymmetric dimethylarginine, smoking, and risk of coronary heart disease in apparently healthy men: prospective analysis from the population-based Monitoring of Trends and Determinants in Cardiovascular Disease/Kooperative Gesundheitsforschung in der Regi
    Renke Maas
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Clin Chem 53:693-701. 2007
    ..We investigated the association between plasma concentrations of ADMA and risk in initially healthy smoking and nonsmoking men...
  30. ncbi request reprint Asymmetrical dimethylarginine (ADMA) and coronary endothelial function in patients with coronary artery disease and mild hypercholesterolemia
    Renke Maas
    Clinical Pharmacology Unit, Institute for Experimental and Clinical Pharmacology and Toxicology, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Atherosclerosis 191:211-9. 2007
    ..To investigate the association of the endogenous nitric oxide synthase inhibitor asymmetrical dimethylarginine (ADMA) and coronary endothelial function...
  31. doi request reprint Isoprostanes inhibit vascular endothelial growth factor-induced endothelial cell migration, tube formation, and cardiac vessel sprouting in vitro, as well as angiogenesis in vivo via activation of the thromboxane A(2) receptor: a potential link between ox
    Ralf A Benndorf
    Clinical Pharmacology Unit, Department of Pharmacology, University Hospital Hamburg, Hamburg, Germany
    Circ Res 103:1037-46. 2008
    ..By this mechanism, isoprostanes may contribute directly to exacerbation of coronary heart disease and to capillary rarefaction in disease states of increased oxidative stress...
  32. ncbi request reprint Determination of asymmetric dimethylarginine (ADMA) using a novel ELISA assay
    Friedrich Schulze
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, Center for Experimental Medicine, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Clin Chem Lab Med 42:1377-83. 2004
    ..For human plasma we found a mean of 0.65 micromol/l in healthy subjects. In the plasma of mice and rats we found mean concentrations of 1.05 and 1.09 micromol/l, respectively...
  33. doi request reprint Symmetric dimethylarginine is a marker of detrimental outcome in the acute phase after ischaemic stroke: role of renal function
    Nicole Lüneburg
    Department of Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany
    Clin Sci (Lond) 122:105-11. 2012
    ..Our results strengthen the prognostic value of renal function in patients with stroke and confirm the hypothesis that SDMA is a promising marker for renal function...
  34. doi request reprint The L-Arginine-asymmetric dimethylarginine ratio is an independent predictor of mortality in dilated cardiomyopathy
    Maike Anderssohn
    Department of Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    J Card Fail 18:904-11. 2012
    ..Asymmetric dimethylarginine (ADMA) is associated with increased mortality in patients with chronic heart failure but it remains unclear if the etiology of heart failure influences the prognostic value of dimethylarginines...
  35. pmc Myeloperoxidase deficiency preserves vasomotor function in humans
    Tanja K Rudolph
    Cardiovascular Research Center and Department of General and Interventional Cardiology, University Heart Center Hamburg, Hamburg, Germany
    Eur Heart J 33:1625-34. 2012
    ..Observational studies have suggested a mechanistic link between the leucocyte-derived enzyme myeloperoxidase (MPO) and vasomotor function. Here, we tested whether MPO is systemically affecting vascular tone in humans...
  36. ncbi request reprint Asymmetric dimethylarginine determines the improvement of endothelium-dependent vasodilation by simvastatin: Effect of combination with oral L-arginine
    Gerhild I Böger
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, Hamburg, Germany
    J Am Coll Cardiol 49:2274-82. 2007
    ..We hypothesized that the level of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of endothelial nitric oxide (NO) synthase (eNOS), might determine the endothelial effects of statins...
  37. doi request reprint Polymorphisms in the promoter region of the dimethylarginine dimethylaminohydrolase 2 gene are associated with prevalence of hypertension
    Renke Maas
    Institute of Experimental and Clinical Pharmacology and Toxicology, Friedrich Alexander University, Erlangen Nuremberg, Germany
    Pharmacol Res 60:488-93. 2009
    ..The mechanisms of this association need further investigation...
  38. ncbi request reprint Elevation of asymmetrical dimethylarginine (ADMA) and coronary artery disease in men with erectile dysfunction
    Renke Maas
    Institut fur Experimentelle und Klinische Pharmakologie, Universitatsklinikum Hamburg Eppendorf, Germany
    Eur Urol 48:1004-11; discussion 1011-2. 2005
    ....
  39. ncbi request reprint Elevated levels of asymmetric dimethylarginine (ADMA) as a marker of cardiovascular disease and mortality
    Rainer H Boger
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Clin Chem Lab Med 43:1124-9. 2005
    ..Recently, advances have been made in the development of analytical methods that are reliable and fast enough to allow determination of ADMA in clinical routine...
  40. doi request reprint Aliskiren accumulation in the kidney: no major role for binding to renin or prorenin
    Sascha Lange
    Division of Nephrology, Department of Medicine, University Hospital Hamburg Eppendorf, Hamburg, Germany
    J Hypertens 31:713-9. 2013
    ..This may be attributable to drug accumulation in the kidney as recently shown in rats and mice. Since aliskiren binds to renin we examined in the present study whether this accumulation depends on the renin content of the kidney...
  41. doi request reprint Dimethylarginines, homocysteine metabolism, and cerebrospinal fluid markers for Alzheimer's disease
    Sönke Arlt
    Department of Psychiatry and Psychotherapy, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    J Alzheimers Dis 31:751-8. 2012
    ..Functional and interventional studies are needed to further proof this hypothesis...
  42. ncbi request reprint A stable-isotope based technique for the determination of dimethylarginine dimethylaminohydrolase (DDAH) activity in mouse tissue
    Renke Maas
    Institute of Experimental and Clinical Pharmacology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 851:220-8. 2007
    ..9% and 10% for mouse kidney and liver, respectively. The present DDAH activity assay allows for the first time to simultaneously determine DDAH activity and endogenous formation of ADMA, SDMA, and L-arginine in tissue...
  43. doi request reprint Pathogenic cycle between the endogenous nitric oxide synthase inhibitor asymmetrical dimethylarginine and the leukocyte-derived hemoprotein myeloperoxidase
    Eike Christin von Leitner
    Department of Interventional Cardiology, Hamburg University Heart Center, Hamburg, Germany
    Circulation 124:2735-45. 2011
    ..The aim of the study was to investigate a potential 2-way interaction between ADMA and MPO...
  44. ncbi request reprint Asymmetric dimethylarginine is an independent risk factor for coronary heart disease: results from the multicenter Coronary Artery Risk Determination investigating the Influence of ADMA Concentration (CARDIAC) study
    Friedrich Schulze
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, Center of Experimental Medicine, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Am Heart J 152:493.e1-8. 2006
    ..It has been shown that ADMA predicts cardiovascular mortality in patients who have coronary heart disease (CHD). However, the question whether ADMA is an independent risk factor for CHD still remains unresolved...
  45. ncbi request reprint ADMA and oxidative stress may relate to the progression of renal disease: rationale and design of the VIVALDI study
    Rainer H Boger
    Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    Vasc Med 10:S97-102. 2005
    ....
  46. doi request reprint Asymmetric dimethylarginine predicts outcome and time of stay in hospital in patients attending an internal medicine emergency room
    Friedrich Schulze
    University Hospital Hamburg Eppendorf, Institute for Experimental and Clinical Pharmacology and Toxicology, Hamburg, Germany
    Clin Chim Acta 401:20-4. 2009
    ..We investigated the predictive value of asymmetric dimethylarginine (ADMA) in unselected patients attending an internal medicine ER regarding outcome of the patients and duration of stay in the hospital...
  47. doi request reprint The role of nitric oxide synthase inhibition by asymmetric dimethylarginine in the pathophysiology of preeclampsia
    Rainer H Boger
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf Martinistrasse 52, DE 20246 Hamburg, Germany boeger uke uni hamburg de
    Gynecol Obstet Invest 69:1-13. 2010
    ..Recently, diagnostic assays for ADMA, e.g. by ELISA, have become available, which render ADMA a possible biomarker to identify pregnant women at risk of developing preeclampsia...
  48. doi request reprint Asymmetric dimethylarginine (ADMA) as a prospective marker of cardiovascular disease and mortality--an update on patient populations with a wide range of cardiovascular risk
    Rainer H Boger
    Institute of Experimental and Clinical Pharmacology, University Medical Center Hamburg Eppendorf, Germany
    Pharmacol Res 60:481-7. 2009
    ....
  49. ncbi request reprint Determination of NG,NG-dimethyl-L-arginine, an endogenous NO synthase inhibitor, by gas chromatography-mass spectrometry
    Jennifer Albsmeier
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 809:59-65. 2004
    ..This method is fast, rugged and an alternative to high performance liquid chromatography (HPLC) analysis of ADMA in cell culture supernatants and small volumes of human plasma...
  50. ncbi request reprint ADMA and oxidative stress are responsible for endothelial dysfunction in hyperhomocyst(e)inemia: effects of L-arginine and B vitamins
    Karsten Sydow
    Department of Cardiology, University Hospital Hamburg Eppendorf, Martinistr 52, 20246 Hamburg, Germany
    Cardiovasc Res 57:244-52. 2003
    ..We investigated whether oral treatment with B vitamins or L-arginine normalizes endothelium-dependent, flow-dependent vasodilation (FDD) in patients with peripheral arterial occlusive disease (PAOD) and hyperhomocyst(e)inemia...
  51. doi request reprint AT1 antagonism and renin inhibition in mice: pivotal role of targeting angiotensin II in chronic kidney disease
    Christoph Fraune
    Div of Nephrology, Dept of Medicine, Universitatsklinikum Hamburg Eppendorf, Martinistraβe 52, 20246 Hamburg, Germany
    Am J Physiol Renal Physiol 303:F1037-48. 2012
    ..These observations underscore the pivotal role of targeting ANG II to reduce renal injury...
  52. doi request reprint Asymmetric dimethylarginine as a mediator of vascular dysfunction and a marker of cardiovascular disease and mortality: an intriguing interaction with diabetes mellitus
    Maike Anderssohn
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Diab Vasc Dis Res 7:105-18. 2010
    ..Anticipated advances in clinical and experimental investigation will help us to better understand this complex interrelationship between diabetes, eNOS, DDAH and ADMA...
  53. pmc P2Y12 polymorphisms and antiplatelet effects of aspirin in patients with coronary artery disease
    Anneke Bierend
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Br J Clin Pharmacol 65:540-7. 2008
    ....
  54. ncbi request reprint Clinical pharmacokinetics of antioxidants and their impact on systemic oxidative stress
    Edzard Schwedhelm
    Institute of Experimental and Clinical Pharmacology, Clinical Pharmacology Unit, University Hospital of Hamburg Eppendorf, Hamburg, Germany
    Clin Pharmacokinet 42:437-59. 2003
    ..Catechins are degraded to several gamma-valerolactone derivatives and phase II conjugates have also been identified. Only limited clinical pharmacokinetic data for other polyphenols such as resveratrol have been reported to date...
  55. doi request reprint Stable isotope dilution assay for liquid chromatography-tandem mass spectrometric determination of L-homoarginine in human plasma
    Dorothee Atzler
    Department of Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 879:2294-8. 2011
    ..4 ± 4.5% (±SD) for 0.1-50 μmol/L and 7.5 ± 2.0% for 2 and 5 μmol/L, respectively. Applying this method, a mean plasma concentration of L-homoarginine of 2.5 ± 1.0 μmol/L was determined in 136 healthy humans...
  56. ncbi request reprint FK778 attenuates lymphocyte-endothelium interaction after cardiac transplantation: in vivo and in vitro studies
    Tobias Deuse
    Department of Cardiovascular Surgery, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Transplantation 78:71-7. 2004
    ..We investigated the effects of FK778 upon acute cardiac allograft rejection and upon adhesion molecule upregulation in experimental transplantation and by using in vitro cell culture...
  57. doi request reprint Angiotensin II type 2 receptor deficiency aggravates renal injury and reduces survival in chronic kidney disease in mice
    Ralf A Benndorf
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Toxicology and Pharmacology, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Kidney Int 75:1039-49. 2009
    ..Whether activation of the AT2 receptor will have therapeutic benefit in chronic kidney disease will require further study...
  58. ncbi request reprint Acetylsalicylic acid inhibits monocyte adhesion to endothelial cells by an antioxidative mechanism
    Guido Eisele
    Clinical Pharmacology Unit, Institute of Experimental and Clinical Pharmacology, University Hospital Hamburg Eppendorf, Germany
    J Cardiovasc Pharmacol 43:514-21. 2004
    ..For ASA, the observed inhibition of monocyte adhesion was accomplished with concentrations that can be reached after single oral doses of 500 mg of ASA...
  59. ncbi request reprint The pathophysiology of erectile dysfunction related to endothelial dysfunction and mediators of vascular function
    Renke Maas
    Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie, Universitatsklinikum Hamburg Eppendorf, Hamburg, Germany
    Vasc Med 7:213-25. 2002
    ..Likewise, the role of endothelium-derived factors mediating NO-independent pathways is evaluated...
  60. pmc Impact of atorvastatin treatment on platelet-activating factor acetylhydrolase and 15-F(2trans)-isoprostane in hypercholesterolaemic patients
    Ghainsom D Kom
    Institute of Experimental and Clinical Pharmacology and Toxicology, University Medical Centre Hamburg Eppendorf, Hamburg, Germany
    Br J Clin Pharmacol 63:672-9. 2007
    ..We investigated the effect of atorvastatin on PLA(2)s and PAF-AH activity and the urinary excretion of 15-F(2trans)-isoprostane (15-F(2t)-IsoP, 8-iso-PGF(2alpha), iPF(2alpha)-III)...
  61. doi request reprint The role of asymmetric and symmetric dimethylarginines in renal disease
    Edzard Schwedhelm
    Institute of Clinical Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Martinistrasse 52, 20246 Hamburg, Germany
    Nat Rev Nephrol 7:275-85. 2011
    ..The biochemical pathways that regulate ADMA and SDMA, and the pathways that transduce their biological function, could be targeted to treat renal disease in the future...
  62. ncbi request reprint Comparison of HPLC method and commercial ELISA assay for asymmetric dimethylarginine (ADMA) determination in human serum
    Friedrich Schulze
    J Chromatogr B Analyt Technol Biomed Life Sci 831:333. 2006
  63. ncbi request reprint AT1-receptor blockade with irbesartan improves peripheral but not coronary endothelial dysfunction in patients with stable coronary artery disease
    Ascan Warnholtz
    Department of Medicine II, Johannes Gutenberg University Mainz, Langenbeckstrasse 1, 55101 Mainz, Germany
    Atherosclerosis 194:439-45. 2007
    ..Since reduced FMD of the brachial artery has been shown to be associated with a high-cardiovascular event rate, improvement of FMD by IRB may lead to better prognosis of patients with CAD...
  64. ncbi request reprint Divergence in urinary 8-iso-PGF(2alpha) (iPF(2alpha)-III, 15-F(2t)-IsoP) levels from gas chromatography-tandem mass spectrometry quantification after thin-layer chromatography and immunoaffinity column chromatography reveals heterogeneity of 8-iso-PGF(2al
    Dimitrios Tsikas
    Institute of Clinical Pharmacology, Hannover Medical School, D 30625 Hannover, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 794:237-55. 2003
    ..We assume that ent-15(S)-8-iso-PGF(2alpha) is also contributing by approximately 50% to urinary 8-iso-PGF(2alpha). This finding may have methodological, mechanistic and clinical implications...
  65. ncbi request reprint Plasma concentrations of asymmetric dimethylarginine (ADMA) in metabolic syndrome
    Ronald G Garcia
    Int J Cardiol 122:176-8. 2007
    ..Ethnic-specific or environmental differences in the etiologic mechanisms of metabolic syndrome need to be elucidated in further studies...
  66. ncbi request reprint Comparative effects of AT1-antagonism and angiotensin-converting enzyme inhibition on markers of inflammation and platelet aggregation in patients with coronary artery disease
    Bernhard Schieffer
    Department of Cardiology and Angiology, Medizinische Hochschule Hannover, Germany
    J Am Coll Cardiol 44:362-8. 2004
    ..We evaluated whether renin-angiotensin system (RAS) blockade attenuates cardiovascular events...
  67. ncbi request reprint Postocclusive reactive hyperemia inversely correlates with urinary 15-F2t-isoprostane levels in systemic sclerosis
    Jean Luc Cracowski
    INSERM ESPRI HP2 Laboratory, EA 3745, Grenoble Medical School, Grenoble, France
    Free Radic Biol Med 40:1732-7. 2006
    ..Whether oxygen free radicals initiate the vascular dysfunction or whether there is an initial trigger that initiates both processes will need to be further clarified in future studies...
  68. ncbi request reprint Accurate quantification of basal plasma levels of 3-nitrotyrosine and 3-nitrotyrosinoalbumin by gas chromatography-tandem mass spectrometry
    Dimitrios Tsikas
    Institute of Clinical Pharmacology, Hannover Medical School, Carl Neuberg Strasse 1, 30625, Hannover, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 784:77-90. 2003
    ..9+/-3.1 for NO(2)Tyr and 1.2+/-0.2 for NO(2)TyrALB/TyrALB. The present GC-tandem MS method provides accurate values of NO(2)Tyr and NO(2)TyrALB in human plasma...
  69. ncbi request reprint Plasma concentrations of asymmetric dimethylarginine (ADMA) in Colombian women with pre-eclampsia
    Renke Maas
    JAMA 291:823-4. 2004
  70. ncbi request reprint Methodological considerations on the detection of 3-nitrotyrosine in the cardiovascular system
    Dimitrios Tsikas
    Circ Res 90:E70. 2002
  71. ncbi request reprint Unusual collision-induced dissociation of fluorated and non-fluorated alpha-nitrotoluene analogs in a gas chromatograph triple-stage quadrupole mass spectrometer under electron-capturing negative-ion chemical ionization conditions
    Dimitrios Tsikas
    Institute of Clinical Pharmacology, Hannover Medical School, Carl Neuberg Strasse 1, D 30623 Hannover, Germany
    J Chromatogr A 1067:337-45. 2005
    ..The unique CID behavior of C6F5CH2--NO2 can be utilized to unequivocally identify and accurately quantify nitrite in biological fluids by GC-tandem MS...