Claudia Schoch

Summary

Affiliation: University of Munich
Country: Germany

Publications

  1. ncbi request reprint Detailed analysis of FLT3 expression levels in acute myeloid leukemia
    Florian Kuchenbauer
    Department of Internal Medicine III, Ludwig Maximilians University of Munich, University Hospital Grosshadern, Munich, Germany
    Haematologica 90:1617-25. 2005
  2. ncbi request reprint Patients with de novo acute myeloid leukaemia and complex karyotype aberrations show a poor prognosis despite intensive treatment: a study of 90 patients
    C Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Br J Haematol 112:118-26. 2001
  3. ncbi request reprint The influence of age on prognosis of de novo acute myeloid leukemia differs according to cytogenetic subgroups
    Claudia Schoch
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Haematologica 89:1082-90. 2004
  4. ncbi request reprint AML with 11q23/MLL abnormalities as defined by the WHO classification: incidence, partner chromosomes, FAB subtype, age distribution, and prognostic impact in an unselected series of 1897 cytogenetically analyzed AML cases
    Claudia Schoch
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University of Munich, Marchioninistr 15, 81377 Munchen, Germany
    Blood 102:2395-402. 2003
  5. ncbi request reprint Karyotype is an independent prognostic parameter in therapy-related acute myeloid leukemia (t-AML): an analysis of 93 patients with t-AML in comparison to 1091 patients with de novo AML
    C Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Leukemia 18:120-5. 2004
  6. ncbi request reprint Rapid diagnostic approach to PML-RARalpha-positive acute promyelocytic leukemia
    Claudia Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Hematol J 3:259-63. 2002
  7. ncbi request reprint Cytogenetics in acute myeloid leukemia
    Claudia Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistr 15, 81377 Munich, Germany
    Curr Oncol Rep 4:390-7. 2002
  8. ncbi request reprint Gain of an isochromosome 5p: a new recurrent chromosome abnormality in acute monoblastic leukemia
    C Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistrass 15, 81377 Munich, Germany
    Cancer Genet Cytogenet 127:85-8. 2001
  9. pmc Acute myeloid leukemias with reciprocal rearrangements can be distinguished by specific gene expression profiles
    Claudia Schoch
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, 81366 Munich, Germany
    Proc Natl Acad Sci U S A 99:10008-13. 2002
  10. ncbi request reprint Prognostic impact of early response to induction therapy as assessed by multiparameter flow cytometry in acute myeloid leukemia
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Dept of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, 81366 Muenchen, Germany
    Haematologica 89:528-40. 2004

Collaborators

Detail Information

Publications80

  1. ncbi request reprint Detailed analysis of FLT3 expression levels in acute myeloid leukemia
    Florian Kuchenbauer
    Department of Internal Medicine III, Ludwig Maximilians University of Munich, University Hospital Grosshadern, Munich, Germany
    Haematologica 90:1617-25. 2005
    ..Although new FLT3 mutations are being increasing by investigated, the role of FLT3 expression levels in wild type as well as in mutated FLT3 has only been infrequently addressed...
  2. ncbi request reprint Patients with de novo acute myeloid leukaemia and complex karyotype aberrations show a poor prognosis despite intensive treatment: a study of 90 patients
    C Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Br J Haematol 112:118-26. 2001
    ..However, long-term survival rates are low and alternative treatment strategies for remission induction and consolidation are urgently needed...
  3. ncbi request reprint The influence of age on prognosis of de novo acute myeloid leukemia differs according to cytogenetic subgroups
    Claudia Schoch
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Haematologica 89:1082-90. 2004
    ..The 1225 patients with de novo AML were separated according to age as follows: A1: 16 to 49 years (n=442), A2: 50 to 59 years (n=246), A3: 60-69 years (n=333), A4: 70 years and older (n=204)...
  4. ncbi request reprint AML with 11q23/MLL abnormalities as defined by the WHO classification: incidence, partner chromosomes, FAB subtype, age distribution, and prognostic impact in an unselected series of 1897 cytogenetically analyzed AML cases
    Claudia Schoch
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University of Munich, Marchioninistr 15, 81377 Munchen, Germany
    Blood 102:2395-402. 2003
    ..0 vs 8.9 months, P =.36). In conclusion, the category AML with 11q23/MLL abnormalities accounts for 2.8% of unselected AML, is closely associated with monocytic differentiation, and has a dismal prognosis. (..
  5. ncbi request reprint Karyotype is an independent prognostic parameter in therapy-related acute myeloid leukemia (t-AML): an analysis of 93 patients with t-AML in comparison to 1091 patients with de novo AML
    C Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Leukemia 18:120-5. 2004
    ..In conclusion, these data indicate that cytogenetics are an important prognostic parameter in t-AML. Furthermore, t-AML is an unfavorable factor independent of cytogenetics with respect to survival...
  6. ncbi request reprint Rapid diagnostic approach to PML-RARalpha-positive acute promyelocytic leukemia
    Claudia Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Hematol J 3:259-63. 2002
    ..The introduction of all trans retinoic acid (ATRA) into treatment protocols has improved the outcome of APL dramatically. Therefore, it is essential to establish the diagnosis of APL as quickly and as reliably as possible...
  7. ncbi request reprint Cytogenetics in acute myeloid leukemia
    Claudia Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistr 15, 81377 Munich, Germany
    Curr Oncol Rep 4:390-7. 2002
    ....
  8. ncbi request reprint Gain of an isochromosome 5p: a new recurrent chromosome abnormality in acute monoblastic leukemia
    C Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistrass 15, 81377 Munich, Germany
    Cancer Genet Cytogenet 127:85-8. 2001
    ..As an isochromosome 5p can be misinterpreted as a deletion 5q, which occurs frequently in AML, fluorescence in situ hybridization with loci specific probes is a helpful method to detect this rare abnormality...
  9. pmc Acute myeloid leukemias with reciprocal rearrangements can be distinguished by specific gene expression profiles
    Claudia Schoch
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, 81366 Munich, Germany
    Proc Natl Acad Sci U S A 99:10008-13. 2002
    ..By using two different strategies for microarray data analyses, this study revealed a unique correlation between AML-specific cytogenetic aberrations and gene expression profiles...
  10. ncbi request reprint Prognostic impact of early response to induction therapy as assessed by multiparameter flow cytometry in acute myeloid leukemia
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Dept of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, 81366 Muenchen, Germany
    Haematologica 89:528-40. 2004
    ..We improved the identification of this parameter by implementing multiparameter flow cytometry to quantify bone marrow cells carrying leukemia-associated immunophenotypes (LAIP)...
  11. ncbi request reprint Correlation of protein expression and gene expression in acute leukemia
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Ludwig Maximilians University, University Hospital Grosshadern, Department of Internal Medicine III, Munich, Germany
    Cytometry B Clin Cytom 55:29-36. 2003
    ..In the future, diagnostic procedures may include oligonucleotide microarray analysis (MA) to detect expression patterns of large numbers of specific genes...
  12. ncbi request reprint Impact of FLT3 mutations and promyelocytic leukaemia-breakpoint on clinical characteristics and prognosis in acute promyelocytic leukaemia
    Florian Kuchenbauer
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, Ludwig Maximilians University of Munich, University Hospital Grosshadern, Munich, Germany
    Br J Haematol 130:196-202. 2005
    ..6%) and associated with a significant lower overall survival (P = 0.0339). In addition, cases with bcr3 showed a tendency for a worse event-free survival (P = 0.0795) compared with the bcr1 group...
  13. ncbi request reprint Determination of relapse risk based on assessment of minimal residual disease during complete remission by multiparameter flow cytometry in unselected patients with acute myeloid leukemia
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Ludwig Maximilians University, University Hospital Grosshadern, Department of Internal Medicine III, 81366 Muenchen, Germany
    Blood 104:3078-85. 2004
    ..MFC-based quantification of MRD reveals important prognostic information in unselected patients with AML in addition to cytogenetics and should be further evaluated and used in clinical trials...
  14. ncbi request reprint A new prognostic score for patients with acute myeloid leukemia based on cytogenetics and early blast clearance in trials of the German AML Cooperative Group
    Torsten Haferlach
    Ludwig Maximilians University, University Hospital Grosshadern, Dept of Internal Medicine III, Munchen, Germany
    Haematologica 89:408-18. 2004
    ..To refine cytogenetically based risk-stratification in acute myeloid leukemia (AML)...
  15. ncbi request reprint Morphologic dysplasia in de novo acute myeloid leukemia (AML) is related to unfavorable cytogenetics but has no independent prognostic relevance under the conditions of intensive induction therapy: results of a multiparameter analysis from the German AML
    Torsten Haferlach
    Department of Medicine III, Ludwig Maximilians University, Grosshadern, Munich, Germany
    J Clin Oncol 21:256-65. 2003
    ..We also assessed the clinical significance of the recently introduced World Health Organization (WHO) classification for AML, which proposed dysplasia as a new parameter for classification...
  16. ncbi request reprint New score predicting for prognosis in PML-RARA+, AML1-ETO+, or CBFBMYH11+ acute myeloid leukemia based on quantification of fusion transcripts
    Susanne Schnittger
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistrasse 15, 81377 Munich, Germany
    Blood 102:2746-55. 2003
    ..By combining the transcription ratios at these 2 checkpoints, a new powerful prognostic score has been established...
  17. ncbi request reprint Early blast clearance by remission induction therapy is a major independent prognostic factor for both achievement of complete remission and long-term outcome in acute myeloid leukemia: data from the German AML Cooperative Group (AMLCG) 1992 Trial
    Wolfgang Kern
    Ludwig Maximilians University, University Hospital Grosshadern, Department of Internal Medicine III, Muenchen, Germany
    Blood 101:64-70. 2003
    ..0001), LDH (P <.0001), and day 16 blasts (P =.0359). The prognostic significance of day 16 blasts is independent of pretherapeutic parameters and predicts outcome even in patients achieving a CR...
  18. ncbi request reprint Loss of genetic material is more common than gain in acute myeloid leukemia with complex aberrant karyotype: a detailed analysis of 125 cases using conventional chromosome analysis and fluorescence in situ hybridization including 24-color FISH
    Claudia Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Genes Chromosomes Cancer 35:20-9. 2002
    ....
  19. ncbi request reprint Comparison of mRNA abundance quantified by gene expression profiling and percentage of positive cells using immunophenotyping for diagnostic antigens in acute and chronic leukemias
    Wolfgang Kern
    MLL Munich Leukemia Laboratory, Munich, Germany
    Cancer 107:2401-7. 2006
    ....
  20. ncbi request reprint Detection of minimal residual disease in unselected patients with acute myeloid leukemia using multiparameter flow cytometry for definition of leukemia-associated immunophenotypes and determination of their frequencies in normal bone marrow
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Ludwig Maximilians University, University Hospital Grosshadern, Dept of Internal Medicine III, Muenchen, Germany
    Haematologica 88:646-53. 2003
    ..The present analysis aimed at improving the applicability of this approach to more patients with AML...
  21. ncbi request reprint Molecular characterization of acute leukemias by use of microarray technology
    Alexander Kohlmann
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, Ludwig Maximilians University, Munich, Germany
    Genes Chromosomes Cancer 37:396-405. 2003
    ..These data support a possible future application of microarray technology for classification of the acute leukemias...
  22. ncbi request reprint Pattern robustness of diagnostic gene expression signatures in leukemia
    Alexander Kohlmann
    Laboratory for Leukemia Diagnostics, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Genes Chromosomes Cancer 42:299-307. 2005
    ....
  23. ncbi request reprint A comparison of donor lymphocyte infusions or imatinib mesylate for patients with chronic myelogenous leukemia who have relapsed after allogeneic stem cell transplantation
    Martin Weisser
    Department of Internal Medicine III, University of Munich, Klinikum Grosshadern, Munich, Germany
    Haematologica 91:663-6. 2006
    ..Imatinib therapy resulted in a higher incidence of relapse and inferior leukemia-free survival (p=0.006 and p=0.016, respectively). These data suggest that imatinib alone probably does not cure relapse after HSCT...
  24. ncbi request reprint Evaluation of complete disease remission in acute myeloid leukemia: a prospective study based on cytomorphology, interphase fluorescence in situ hybridization, and immunophenotyping during follow-up in patients with acute myeloid leukemia
    Ulrike Bacher
    Laboratory for Leukemia Diagnostics, Department for Internal Medicine III, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany
    Cancer 106:839-47. 2006
    ..The detection of minimal residual disease (MRD) for predicting prognosis and for therapeutic planning still are under discussion...
  25. ncbi request reprint Four-fold staining including CD45 gating improves the sensitivity of multiparameter flow cytometric assessment of minimal residual disease in patients with acute myeloid leukemia
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, Ludwig Maximilians University, University Hospital Grosshadern, 81366 Muenchen, Germany
    Hematol J 5:410-8. 2004
    ..08 log (range, 1.22-4.01) and 2.28 log (range, 1.12-3.34) with and without CD45 gating. CD45 gating improves the sensitivity of MFC-based MRD monitoring in AML by 1 log...
  26. ncbi request reprint Cytogenetic profile in de novo acute myeloid leukemia with FAB subtypes M0, M1, and M2: a study based on 652 cases analyzed with morphology, cytogenetics, and fluorescence in situ hybridization
    Mirjam Klaus
    Department of Internal Medicine III, Laboratory for Leukemia Diagnostics, Ludwig Maximilians University, Marchioninistrasse 15, 81377 Munich, Germany
    Cancer Genet Cytogenet 155:47-56. 2004
    ..In conclusion, t(8;21), +11, +13, and +14 are strongly associated with AML M0, M1, and M2. The FISH screening analyses identified abnormalities in an additional 3% in normal karyotypes...
  27. ncbi request reprint Implications of NRAS mutations in AML: a study of 2502 patients
    Ulrike Bacher
    Munich Leukemia Laboratory, Department of Internal Medicine III, University Hospital Munich, Ludwig Maximilians University, Max Lebsche Platz 31, 81377 Munich, Germany
    Blood 107:3847-53. 2006
    ..However, there was a trend to better survival in most subgroups, especially when other molecular markers (FLT3-LM, MLL-PTD, and NPM) were taken into account...
  28. ncbi request reprint Acute myeloid leukemia with a complex aberrant karyotype is a distinct biological entity characterized by genomic imbalances and a specific gene expression profile
    Claudia Schoch
    Laboratory for Leukemia Diagnostics, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Genes Chromosomes Cancer 43:227-38. 2005
    ..These data may be the basis for developing targeted therapeutic strategies to increase the cure rate in patients with AML and a complex aberrant karyotype...
  29. ncbi request reprint D324N single-nucleotide polymorphism in the FLT3 gene is associated with higher risk of myeloid leukemias
    Susanne Schnittger
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Munich, Germany
    Genes Chromosomes Cancer 45:332-7. 2006
    ..001). In addition, 21 of 234 CML (9.0%) and 7 of 155 ALL (4.5%) cases carried the FLT3 D324N. Our data suggest that the FLT3 D324N variant might be associated with a predisposition to different subtypes of leukemia...
  30. ncbi request reprint Stability of leukemia-associated aberrant immunophenotypes in patients with acute myeloid leukemia between diagnosis and relapse: comparison with cytomorphologic, cytogenetic, and molecular genetic findings
    Daniela Voskova
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Cytometry B Clin Cytom 62:25-38. 2004
    ..Changes in LAIPs during the course of the disease may be a limitation for this approach...
  31. ncbi request reprint Analysis of FLT3 length mutations in 1003 patients with acute myeloid leukemia: correlation to cytogenetics, FAB subtype, and prognosis in the AMLCG study and usefulness as a marker for the detection of minimal residual disease
    Susanne Schnittger
    Department of Internal Medicine III, University of Munich, Germany
    Blood 100:59-66. 2002
    ..6 months; P =.0072) because of a higher relapse rate. Besides the importance of FLT3-LM for biologic and clinical characterization of AML, we show its value as a marker for disease monitoring based on 120 follow-up samples of 34 patients...
  32. ncbi request reprint Role of gene expression profiling for diagnosing acute leukemias
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Munich, Germany
    Rev Clin Exp Hematol 9:E1. 2005
    ..Furthermore, it is anticipated that new biologically defined and clinically relevant subtypes of leukemia will be identified based on gene expression profiling. This method may therefore guide therapeutic decisions...
  33. ncbi request reprint Nucleophosmin gene mutations are predictors of favorable prognosis in acute myelogenous leukemia with a normal karyotype
    Susanne Schnittger
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilian s University, Munich, Germany
    Blood 106:3733-9. 2005
    ..In conclusion, this study demonstrates that NPM1+/FLT3-LM- mutations are an independent predictor for a favorable outcome in AML with normal karyotype...
  34. ncbi request reprint Gene expression profiling as a tool for the diagnosis of acute leukemias
    Torsten Haferlach
    Department of Internal Medicine III, University Hospital Grosshadern, Munich, Germany
    Semin Hematol 40:281-95. 2003
    ..Gene expression profiling should also lead to the detection of new biological and clinically relevant subtypes in leukemia and therefore guide therapeutic decisions...
  35. ncbi request reprint Acute myeloid leukemia (AML) with t(8;21)(q22;q22) relapsing as AML with t(3;21)(q26;q22)
    Ulrike Bacher
    Department of Clinical Chemistry, Ludwig Maximilians University of Munich, Marchioninistrasse 15, 81377 Munich, Germany
    Cancer Genet Cytogenet 168:172-4. 2006
    ..These data suggest that this patient developed a secondary therapy-related AML rather than a relapse...
  36. ncbi request reprint AML M3 and AML M3 variant each have a distinct gene expression signature but also share patterns different from other genetically defined AML subtypes
    Torsten Haferlach
    Laboratory for Leukemia Diagnostics, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Genes Chromosomes Cancer 43:113-27. 2005
    ..0001), may partly contribute to the different phenotypes. However, linear regression analysis demonstrated that genes differentially expressed between M3 and M3v did not correlate with FLT3-LM...
  37. ncbi request reprint Population-based age-specific incidences of cytogenetic subgroups of acute myeloid leukemia
    Ulrike Bacher
    Department for Internal Medicine III, Klinikum, Grosshadern, Ludwig Maximilians University, Munich, Germany
    Haematologica 90:1502-10. 2005
    ..However, detailed data on the population-based age-dependent incidences of distinct cytogenetic subtypes as well as of molecular mutations are lacking...
  38. ncbi request reprint Further correlations of morphology according to FAB and WHO classification to cytogenetics in de novo acute myeloid leukemia: a study on 2,235 patients
    Ulrike Bacher
    Laboratory for Leukemia Diagnostics, Department for Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistr 15, 81377, Munich, Germany
    Ann Hematol 84:785-91. 2005
    ..In conclusion, the central role of morphology as defined by the FAB and WHO classification in AML at diagnosis is still justified in combination with other techniques...
  39. ncbi request reprint KIT-D816 mutations in AML1-ETO-positive AML are associated with impaired event-free and overall survival
    Susanne Schnittger
    Laboratory of Leukemia Diagnostics and Clinical Cooperative Group Leukemia, Department of Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Blood 107:1791-9. 2006
    ..Patients with KIT-D816-positive/AML1-ETO-positive AML might benefit from early intensification of treatment or combination of conventional chemotherapy with KIT PTK inhibitors...
  40. ncbi request reprint Monitoring of minimal residual disease in acute myeloid leukemia
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Ludwig Maximilians University, University Hospital Grosshadern, Department of Internal Medicine III, 81366 Muenchen, Germany
    Crit Rev Oncol Hematol 56:283-309. 2005
    ..Thus, it is desirable to establish new molecular markers for PCR-based studies. Large clinical trials will determine the role and place of immunologic and PCR-based monitoring in the prognostic stratification of patients with AML...
  41. ncbi request reprint Global approach to the diagnosis of leukemia using gene expression profiling
    Torsten Haferlach
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, Ludwig Maximilians University, Marchioninistr 15, 81377 Munich
    Blood 106:1189-98. 2005
    ..Accordingly, cluster analysis completely separated all 13 subgroups analyzed. Gene expression profiling can predict all clinically relevant subentities of leukemia with high accuracy...
  42. ncbi request reprint Risk assessment by monitoring expression levels of partial tandem duplications in the MLL gene in acute myeloid leukemia during therapy
    Martin Weisser
    Laboratory for Leukemia Diagnostics, Medical Department III, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany
    Haematologica 90:881-9. 2005
    ....
  43. ncbi request reprint A new and recurrent activating length mutation in exon 20 of the FLT3 gene in acute myeloid leukemia
    Karsten Spiekermann
    Clinical Cooperative Group Leukemia, GSF, National Research Center for Environment and Health, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistrasse 15, 81377 Munich, Germany
    Blood 100:3423-5. 2002
    ..Our results show for the first time that in addition to known mutations in the JM and the catalytic domain, further activating length mutations exist in the FLT3 gene...
  44. ncbi request reprint Distinct genetic patterns can be identified in acute monoblastic and acute monocytic leukaemia (FAB AML M5a and M5b): a study of 124 patients
    Torsten Haferlach
    Department of Internal Medicine III, Laboratory for Leukaemia Diagnostics, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistrasse 15, 81377 Munich, Germany
    Br J Haematol 118:426-31. 2002
    ..In conclusion, we demonstrated genetic, i.e. biological, differences between AML M5a and AML M5b and all other AML. Therefore, AML M5 should further be categorized as two different groups, as proposed by the WHO classification...
  45. ncbi request reprint Impact of integrating clinical and genetic information
    Martin Dugas
    Department of Medical Informatics, Biometrics and Epidemiology IBE, University of Munich, Marchioninistr 15, D 81377 Munich, Germany
    In Silico Biol 2:383-91. 2002
    ....
  46. ncbi request reprint Gene expression profiling as a diagnostic tool in acute myeloid leukemia
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Munich, Germany
    Am J Pharmacogenomics 4:225-37. 2004
    ..Furthermore, gene expression profiling may also lead to the detection of new biologically defined and clinically relevant subtypes in leukemia and guide therapeutic decision-making in the future...
  47. ncbi request reprint Cytogenetics, fluorescence in situ hybridization, and reverse transcriptase polymerase chain reaction are necessary to clarify the various mechanisms leading to an MLL-AF10 fusion in acute myelocytic leukemia with 10;11 rearrangement
    Mirjam Klaus
    Department of Internal Medicine III, Laboratory for Leukemia Diagnostics, Ludwig Maximilians University, 81377 Munich, Germany
    Cancer Genet Cytogenet 144:36-43. 2003
    ..Compared to translocations involving MLL and other partner genes, complex rearrangements are unique for MLL-AF10 fusions. This may result from the opposite orientation of MLL and AF10...
  48. ncbi request reprint A combination of cytomorphology, cytogenetic analysis, fluorescence in situ hybridization and reverse transcriptase polymerase chain reaction for establishing clonality in cases of persisting hypereosinophilia
    Ulrike Bacher
    Department of Clinical Chemistry, Ludwig Maximilians University, Munich Marchioninistr 15, 81377 Munich
    Haematologica 91:817-20. 2006
    ..A FIP1L1-PDGFRA fusion gene was identified in four male patients by interphase FISH and RT-PCR. These methods in combination demonstrated clonality in 8/40 patients (20%) with a male predominance (6/8; 75%)...
  49. ncbi request reprint FLT3 length mutations as marker for follow-up studies in acute myeloid leukaemia
    Susanne Schnittger
    Laboratory for Leukaemia Diagnostics, Department of Internal Medicine III, Ludwig Maximilians University of Munich, University Hospital Grosshadern, Munich, Germany
    Acta Haematol 112:68-78. 2004
    ..Using conventional PCR it clearly could be shown that for most of the patients positive at presentation FLT3-LM is a reliable PCR marker for monitoring treatment response. Even an early detection of relapse was possible in some cases...
  50. ncbi request reprint The fusion protein AML1-ETO in acute myeloid leukemia with translocation t(8;21) induces c-jun protein expression via the proximal AP-1 site of the c-jun promoter in an indirect, JNK-dependent manner
    Annika Elsasser
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University Munich, Germany
    Oncogene 22:5646-57. 2003
    ..These data suggest a model in which AML1-ETO induces proto-oncogene c-jun expression via the proximal AP-1 site of the c-jun promoter in a JNK-dependent manner...
  51. ncbi request reprint Additional clonal abnormalities in Philadelphia-positive ALL and CML demonstrate a different cytogenetic pattern at diagnosis and follow different pathways at progression
    Ulrike Bacher
    Laboratory for Leukemia Diagnostics, Department for Internal Medicine III, Klinikum Grosshadern, Ludwig Maximilians University, Marchioninistrasse 15, D 81377 Munich, Germany
    Cancer Genet Cytogenet 157:53-61. 2005
    ..In conclusion, we were able to demonstrate that the cytogenetic patterns of Ph+ ALL and of CML are different at diagnosis and furthermore follow different pathways during progression or relapse...
  52. ncbi request reprint Immunostimulatory oligonucleotide-induced metaphase cytogenetics detect chromosomal aberrations in 80% of CLL patients: A study of 132 CLL cases with correlation to FISH, IgVH status, and CD38 expression
    Frank Dicker
    MLL Munich Leukemia Laboratory GmbH, Max Lebsche Platz 31, 81377 Munich, Germany
    Blood 108:3152-60. 2006
    ..We demonstrate that FISH analysis underestimates the complexity of chromosomal aberrations in CLL. Therefore, conventional cytogenetics may define subgroups of patients with high risk of progression...
  53. ncbi request reprint Blast count and cytogenetics correlate and are useful parameters for the evaluation of different phases in chronic myeloid leukemia
    Ulrike Bacher
    Laboratory for Leukemia Diagnostics, Department for Internal Medicine III, Klinikum Grossharden, Ludwig Maximilians University, Marchioninistr, Munich, Germany
    Leuk Lymphoma 46:357-66. 2005
    ..We therefore propose to focus staging systems of CML on the correlation of the percentage of bone marrow blasts and the cytogenetic results...
  54. ncbi request reprint Gain of 9p due to an unbalanced rearrangement der(9;18): a recurrent clonal abnormality in chronic myeloproliferative disorders
    Ulrike Bacher
    Department for Internal Medicine III, Klinikum Grosshadern, Ludwig Maximilians University, Marchioninistr 15, D 81377 Munich, Germany
    Cancer Genet Cytogenet 160:179-83. 2005
    ..The prognostic impact of this unbalanced aberration and of gains of 9p in chronic myeloproliferative disorders remains to be clarified...
  55. ncbi request reprint Modern diagnostics in acute leukemias
    Torsten Haferlach
    Laboratory for Leukemia Diagnostics, Medical Department III, University Hospital Grosshadern, Ludwig Maximilians University, Marchioninistreet 15, 81377 Munich, Germany
    Crit Rev Oncol Hematol 56:223-34. 2005
    ..The results serve as a mandatory prerequisite for individual treatment strategies and for the evaluation of treatment response using especially newly defined and highly specific MRD markers...
  56. ncbi request reprint Conventional cytogenetics of myeloproliferative diseases other than CML contribute valid information
    Ulrike Bacher
    Laboratory for Leukemia Diagnostics, Department for Internal Medicine III, Klinikum Grosshadern, Ludwig Maximilians University, Marchioninistrasse 15, 81377 Munich, Germany
    Ann Hematol 84:250-7. 2005
    ..In ET and in HES the aberration rate was only 3 and 7%, respectively. Thus, cytogenetics can be omitted. However, in some of these cases molecular procedures should be integrated into the routine diagnostic process...
  57. ncbi request reprint The myeloid master regulator transcription factor PU.1 is inactivated by AML1-ETO in t(8;21) myeloid leukemia
    Rajani K Vangala
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University Munich and GSF National Research Center for Environment and Health, Germany
    Blood 101:270-7. 2003
    ..1, however, differentiates AML1-ETO-expressing Kasumi-1 cells to the monocytic lineage. Thus, the function of PU.1 is down-regulated by AML1-ETO in t(8;21) myeloid leukemia, whereas overexpression of PU.1 restores normal differentiation...
  58. ncbi request reprint Functional analysis of apoptosis induction in acute myeloid leukaemia-relevance of karyotype and clinical treatment response
    Jan Braess
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Br J Haematol 126:338-47. 2004
    ..Our data indicate that the mechanism for a broad clinical resistance is not a dysfunctional apparatus per se but rather the consequence of anti-apoptotic regulation impeding otherwise functional apoptotic machinery...
  59. ncbi request reprint Detection of t(14;18)(q32;q21) in B-cell chronic lymphocytic leukemia
    Wolfgang Kern
    Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Muenchen, Germany
    Arch Pathol Lab Med 129:410-1. 2005
    ..Therapy is highly diverse between both diseases. We describe a case with cytomorphologically and immunologically proven B-cell chronic lymphocytic leukemia in which t(14;18)(q32;q21) was found...
  60. ncbi request reprint CD10- pre-B acute lymphoblastic leukemia (ALL) is a distinct high-risk subgroup of adult ALL associated with a high frequency of MLL aberrations: results of the German Multicenter Trials for Adult ALL (GMALL)
    Beate Gleissner
    Department of Internal Medicine III, Campus Benjamin Franklin, Charite Universitatsmedizin, Hindenburgdamm 30, 12200 Berlin, Germany
    Blood 106:4054-6. 2005
    ..Our data identify CD10- cytoplasmic immunoglobulin-positive pre-B ALL as a rare (2.2%) but distinct immuno-subtype of adult ALL that is characterized by a high MLL rearrangement rate and a worse outcome...
  61. ncbi request reprint CD40L stimulation enhances the ability of conventional metaphase cytogenetics to detect chromosome aberrations in B-cell chronic lymphocytic leukaemia cells
    Raymund Buhmann
    GSF, National Research Center for Environment and Health, Genzentrum and Medizinische Klinik III, Klinikum der Universitaet Muenchen Grosshadern, Germany
    Br J Haematol 118:968-75. 2002
    ..1), so far unreported in B-CLL, was detected. Taken together, the results of our study show the potential of CD40L-enhanced metaphase cytogenetics to detect more and new chromosome aberrations in B-CLL...
  62. ncbi request reprint Analysis of site-specific transgene integration following cotransduction with recombinant adeno-associated virus and a rep encodingplasmid
    Nadja A Huttner
    Gene Center of the Ludwig Maximilians University Munich, Feodor Lynen Str 25, 81377 Munich, Germany
    J Gene Med 5:120-9. 2003
    ..One of the most promising features is the ability of wild-type (wt) AAV to integrate site-specifically into human chromosome 19. However, this feature is lost in rAAV vectors due to the removal of the rep-coding sequences...
  63. ncbi request reprint Expression analyses identify MLL as a prominent target of 11q23 amplification and support an etiologic role for MLL gain of function in myeloid malignancies
    Bruce Poppe
    Centre for Medical Genetics, University Hospital Ghent, Belgium
    Blood 103:229-35. 2004
    ..In addition, our results indicate that the transcriptional program associated with MLL rearrangements and MLL overexpression displays significant similarities...
  64. ncbi request reprint 6-Thioguanine, cytarabine, and daunorubicin (TAD) and high-dose cytarabine and mitoxantrone (HAM) for induction, TAD for consolidation, and either prolonged maintenance by reduced monthly TAD or TAD-HAM-TAD and one course of intensive consolidation by seq
    Thomas Buchner
    University Medical Center, Department of Medicine, Hematology and Oncology, University of Muenster, Ulbert Schweitzer Str 33, D 48129 Muenster, Germany
    J Clin Oncol 21:4496-504. 2003
    ..To examine the efficacy of prolonged maintenance chemotherapy versus intensified consolidation therapy for patients with acute myeloid leukemia (AML)...
  65. ncbi request reprint Distinct sequences on 11q13.5 and 11q23-24 are frequently coamplified with MLL in complexly organized 11q amplicons in AML/MDS patients
    Andrea Zatkova
    Institut für Medizinische Biologie, Universitat Wien, Wien, Austria
    Genes Chromosomes Cancer 39:263-76. 2004
    ..Thus, we demonstrated that 11q amplicons in AML/MDS patients display a complex organization and have provided evidence for coamplification of two additional regions on the long arm of chromosome 11 that may harbor candidate target genes...
  66. ncbi request reprint Transient response to imatinib in a chronic eosinophilic leukemia associated with ins(9;4)(q33;q12q25) and a CDK5RAP2-PDGFRA fusion gene
    Christoph Walz
    III Medizinische Universitätsklinik, Fakultät für Klinische Medizin Mannheim der Universität Heidelberg, Mannheim, Germany
    Genes Chromosomes Cancer 45:950-6. 2006
    ....
  67. ncbi request reprint Impact of trisomy 8 on expression of genes located on chromosome 8 in different AML subgroups
    Claudia Schoch
    Munich Leukemia Laboratory MLL, Munich 81377, Germany
    Genes Chromosomes Cancer 45:1164-8. 2006
    ..Therefore, trisomy 8 in AML determines no specific disease characteristic but is a disease modulating secondary event...
  68. ncbi request reprint Increased risk for therapy-associated hematologic malignancies in patients with carcinoma of the breast and combined homozygous gene deletions of glutathione transferases M1 and T1
    Detlef Haase
    Department of Hematology and Oncology, Georg August University, Robert Koch Strasse 40, 37075 Gottingen, Germany
    Leuk Res 26:249-54. 2002
    ..An insufficient detoxification of cytostatic drugs such as cyclophosphamide is suggested to represent the underlying pathomechanism...
  69. ncbi request reprint Reverse transcriptase-polymerase chain reaction based quantification of the combined MDS-EVI1/EVI1 gene in acute myeloid leukemia
    Martin Weisser
    Leuk Lymphoma 47:2645-7. 2006
  70. ncbi request reprint Double induction containing either two courses or one course of high-dose cytarabine plus mitoxantrone and postremission therapy by either autologous stem-cell transplantation or by prolonged maintenance for acute myeloid leukemia
    Thomas Buchner
    Department of Medicine, Hematology Oncology, University of Muenster, Muenster, Germany
    J Clin Oncol 24:2480-9. 2006
    ..Whether additional intensification can add to this effect has not yet been determined...
  71. ncbi request reprint Prognosis in therapy-related acute myeloid leukemia and impact of karyotype
    Wolfgang Kern
    J Clin Oncol 22:2510-1. 2004
  72. ncbi request reprint Acute myeloid leukemia with recurring chromosome abnormalities as defined by the WHO-classification: incidence of subgroups, additional genetic abnormalities, FAB subtypes and age distribution in an unselected series of 1,897 patients with acute myeloid l
    Claudia Schoch
    Haematologica 88:351-2. 2003
  73. pmc Computer aided analysis of additional chromosome aberrations in Philadelphia chromosome positive acute lymphoblastic leukaemia using a simplified computer readable cytogenetic notation
    Jutta Bradtke
    Institute of Clinical Genetics, Philipps University, Bahnhofstrabetae 7, Marburg, 35037, Germany
    BMC Bioinformatics 4:4. 2003
    ..The patterns of the karyotype alterations may provide insights into the genetic pathways of disease progression...
  74. ncbi request reprint XIAP expression correlates with monocytic differentiation in adult de novo AML: impact on prognosis
    Ingo Tamm
    Department of Hematology and Oncology, Universitatsmedizin Berlin, Charite, Berlin, Germany
    Hematol J 5:489-95. 2004
    ..We conclude that XIAP but not other IAP family members is associated with monocytic differentiation in normal and malignant myelopoiesis, and may be of prognostic significance for overall survival in adult de novo AML...
  75. pmc Diagnostic tool for the identification of MLL rearrangements including unknown partner genes
    Claus Meyer
    Institute of Pharmaceutical Biology Center for Drug Research, Development and Safety ZAFES, Biocenter, University of Frankfurt, D 60439 Frankfurt Main, Germany
    Proc Natl Acad Sci U S A 102:449-54. 2005
    ..Furthermore, the determined patient-specific fusion sequences are useful for minimal residual disease monitoring of MLL associated acute leukemias...
  76. ncbi request reprint Distinct gene expression patterns associated with FLT3- and NRAS-activating mutations in acute myeloid leukemia with normal karyotype
    Kai Neben
    Division of Molecular Genetics B060, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Oncogene 24:1580-8. 2005
    ..In conclusion, we showed that unique gene expression patterns can be correlated with FLT3-ITD and FLT3-TKD. This might lead to the identification of further pathogenetic relevant candidate genes particularly in AML with normal karyotype...
  77. ncbi request reprint Response and resistance in 300 patients with BCR-ABL-positive leukemias treated with imatinib in a single center: a 4.5-year follow-up
    Tanja Lahaye
    III Medizinische Klinik Mannheim der Universität Heidelberg, Mannheim, Germany
    Cancer 103:1659-69. 2005
    ..The advent of imatinib has considerably changed the treatment of chronic myeloid leukemia (CML). Early studies demonstrated high rates of hematologic and cytogenetic responses in all phases of the disease after limited observation periods...
  78. ncbi request reprint Treatment of older patients with AML
    Thomas Buchner
    University Hospital, Department of Medicine, Hematology and Oncology, Albert Schweitzer Str 33, D 48129 Munster, Germany
    Crit Rev Oncol Hematol 56:247-59. 2005
    ..This finding did not vary by age under or above 60 years. Given the actual median age in this disease being more than 60 years the adequate management of older age AML remains as the major challenge...
  79. ncbi request reprint The t(8;9)(p22;p24) is a recurrent abnormality in chronic and acute leukemia that fuses PCM1 to JAK2
    Andreas Reiter
    III Medizinische Universitätsklinik, Fakultät für Klinische Medizin Mannheim der Universität Heidelberg, Mannheim, Germany
    Cancer Res 65:2662-7. 2005
    ..We conclude that human autoantigen pericentriolar material (PCM1)-JAK2 is a novel, recurrent fusion gene in hematologic malignancies. Patients with PCM1-JAK2 disease are attractive candidates for targeted signal transduction therapy...
  80. ncbi request reprint Effect of histone deacetylase inhibitor valproic acid on progenitor cells of acute myeloid leukemia
    Gesine Bug
    Medizinische Klinik II, Abt Hämatologie Onkologie, Klinikum der Johann Wolfgang Goethe Universitat Frankfurt, Theodor Stern Kai 7, Frankfurt, Germany
    Haematologica 92:542-5. 2007
    ..Our data suggest a differential effect of VPA on the small population of AML progenitor cells and the bulk of aberrantly differentiated blasts in the majority of AML samples tested...