Research Topics
Genomes and Genes
| G SchmitzSummaryAffiliation: University of Regensburg Country: Germany Publications
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Detail Information
Publications
E-LDL and Ox-LDL differentially regulate ceramide and cholesterol raft microdomains in human MacrophagesMargot Grandl
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93042 Regensburg, Germany
Cytometry A 69:189-91. 2006..Formation of different rafts may have consequences for raft associated signaling in cholesterol homeostasis and apoptosis in human macrophages...
The surfactant protein C mutation A116D alters cellular processing, stress tolerance, surfactant lipid composition, and immune cell activationRalf Zarbock
Childrens Hospital of the Ludwig Maximilians University, Lindwurmstr, 4, 80337 Munich, Germany
BMC Pulm Med 12:15. 2012..In this study, we investigated the effects of the mutation A116D in the BRICHOS domain of SP-C on cellular homeostasis. We also evaluated the ability of drugs currently used in ILD therapy to counteract these effects...
Over-stimulation of insulin/IGF-1 signaling by western diet may promote diseases of civilization: lessons learnt from laron syndromeBodo C Melnik
Department of Dermatology, Environmental Medicine and Health Theory, University of Osnabruck, Sedanstrasse 115, D 49090 Osnabrück, Germany
Nutr Metab (Lond) 8:41. 2011....
A non-BRICHOS surfactant protein c mutation disrupts epithelial cell function and intercellular signalingMarkus Woischnik
Department of Pneumology, Dr von Hauner Children s Hospital, Ludwig Maximilians University, Lindwurmstr 4, Munich 80337, Germany
BMC Cell Biol 11:88. 2010..The most frequent SFTPC mutation in ILD patients leads to a threonine for isoleucine substitution at position 73 (I73T) of the SP-C preprotein (proSP-C), however little is known about the cellular consequences of SP-CI73T expression...
Analyzing M-CSF dependent monocyte/macrophage differentiation: expression modes and meta-modes derived from an independent component analysisDominik Lutter
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, D 93042 Regensburg, Germany
BMC Bioinformatics 9:100. 2008..In this study, we investigate the relevance of ICA in gaining new insights into well characterized regulatory mechanisms of M-CSF dependent macrophage differentiation...
Intracellular cholesterol and phospholipid trafficking: comparable mechanisms in macrophages and neuronal cellsG Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Neurochem Res 26:1045-68. 2001....
Role of ABCG1 and other ABCG family members in lipid metabolismG Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
J Lipid Res 42:1513-20. 2001..In addition, new implications for the transcriptional control, as well as the intracellular routing and localization, of these proteins are presented...
Drug evaluation: OGX-011, a clusterin-inhibiting antisense oligonucleotideGerd Schmitz
University of Regensburg, Institute of Clinical Chemistry and Laboratory Medicine, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
Curr Opin Mol Ther 8:547-54. 2006..Phase II clinical trials of OGX-011 in combination with chemotherapeutic drugs are underway in NSCLC, prostate and breast cancer...
Pharmacogenomics: implications for laboratory medicineG Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, D 93042 Regensburg, Germany
Clin Chim Acta 308:43-53. 2001..Therefore, this review focuses on the implications of pharmacogenomics on the clinical laboratory...
Role of redox regulation and lipid rafts in macrophages during Ox-LDL-mediated foam cell formationGerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
Antioxid Redox Signal 9:1499-518. 2007....
The opposing effects of n-3 and n-6 fatty acidsGerd Schmitz
Institute of Clinical Chemistry, University of Regensburg, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
Prog Lipid Res 47:147-55. 2008..In addition, the n-3/n-6 PUFA content of cell and organelle membranes, as well as membrane microdomains strongly influences membrane function and numerous cellular processes such as cell death and survival...
ABC transporters and cholesterol metabolismG Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93042 Regensburg, Germany
Front Biosci 6:D505-14. 2001..Expression of a large number of ABC transporters in monocytes/macrophages and their regulation by cholesterol flux render these transporter molecules potentially critical players in chronic inflammatory diseases such as atherosclerosis...
Genomic organization of the human cholesterol-responsive ABC transporter ABCA7: tandem linkage with the minor histocompatibility antigen HA-1 geneW E Kaminski
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, D 93042, Germany
Biochem Biophys Res Commun 278:782-9. 2000..3...
ABC transporters in cellular lipid traffickingG Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Curr Opin Lipidol 11:493-501. 2000....
Lipidomic strategies to study structural and functional defects of ABC-transporters in cellular lipid traffickingGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, D 93053, Germany
FEBS Lett 580:5597-610. 2006....
Metabolic learning in the intestine: adaptation to nutrition and luminal factorsG Schmitz
Institute of Clinical Chemistry, University of Regensburg, Regensburg, Germany
Horm Metab Res 38:452-4. 2006....
ABCA2: a candidate regulator of neural transmembrane lipid transportG Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Cell Mol Life Sci 59:1285-95. 2002..The unique expression profile together with available structural data suggest roles for this largest known ABC protein in neural transmembrane lipid export...
ATP-binding cassette transporters in macrophages: promising drug targets for treatment of cardiovascular diseaseGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Curr Opin Investig Drugs 3:853-8. 2002..Future studies clarifying the processes involved in the ABCA1 pathway at the cellular level are expected to identify new and possibly more specific pharmaceutical targets...
ABCA1: regulation, trafficking and association with heteromeric proteinsGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Ann Med 34:334-47. 2002..Distinct prebeta-HDL ligand-specific receptor-clusters are involved that may modulate specific signaling pathways with varying outcomes related to prebeta-HDL particle composition, the cell-type and the cellular response status...
Pharmacogenomics and pharmacogenetics of cholesterol-lowering therapyGerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Clin Chem Lab Med 41:581-9. 2003..This review summarizes current knowledge of the pharmacology and the pharmacogenomics of statin therapy...
Zinc finger protein ZNF202 structure and function in transcriptional control of HDL metabolismGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Curr Opin Lipidol 15:199-208. 2004..Here we summarize the structure, regulation and modulation of ZNF202 function by protein interactions...
Transcriptional regulatory networks in lipid metabolism control ABCA1 expressionGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
Biochim Biophys Acta 1735:1-19. 2005..Thus, a highly sophisticated transcriptional network controls the balanced expression of ABCA1...
High-density lipoproteins and ATP-binding cassette transporters as targets for cardiovascular drug therapyGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Curr Opin Investig Drugs 6:907-19. 2005....
ATP-binding cassette (ABC) transporters in atherosclerosisGerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, Germany
Curr Atheroscler Rep 4:243-51. 2002....
Pharmacogenomics of cholesterol-lowering therapyGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Straub Allee 11, 93042 Regensburg, Germany
Vascul Pharmacol 44:75-89. 2006..In the future, pharmacogenomic testing may allow risk stratification of patients to avoid serious side effects and enable clinicians to select lipid-lowering drugs with the highest efficacy resulting in the best response to therapy...
Molecular defects in the ABCA1 pathway affect platelet functionGerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Pathophysiol Haemost Thromb 35:166-74. 2006..It is concluded that the presence of ABCA1 and ABCG1 in the AP-3 pathway will have major impact for membrane phospholipid processing and HDL metabolism and their relation to disorders of lysosome-related organelles...
CD14 signalling in lipid rafts: new ligands and co-receptorsGerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, D 93053 Regensburg, Germany
Curr Opin Lipidol 13:513-21. 2002..The purpose of this review is to summarize the recent advances in our understanding of CD14-dependent receptor clustering and its relevance in atherogenesis...
Lipid homeostasis in macrophages - implications for atherosclerosisG Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
Rev Physiol Biochem Pharmacol 160:93-125. 2008....
Endolysosomal phospholipidosis and cytosolic lipid droplet storage and release in macrophagesGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, 93049 Regensburg, Germany
Biochim Biophys Acta 1791:524-39. 2009....
Recent advances in molecular genetics of cardiovascular disorders. Implications for atherosclerosis and diseases of cellular lipid metabolismG Schmitz
University of Regensburg, Institute for Clinical Chemistry and Laboratory Medicine, Regensburg, Germany
Pathol Oncol Res 4:152-60. 1998..This will reduce the overall costs of health care without reducing the quality...
The Danubian Biobank projectGerd Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Stud Health Technol Inform 134:143-59. 2008..In addition to local centers three regional DNA, plasma, and tissue banks in Regensburg, Vienna, and Budapest store samples and encrypted patient data for scientific purposes...
Metabolism and atherogenic disease association of lysophosphatidylcholineGerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Atherosclerosis 208:10-8. 2010....
The molecular mechanisms of HDL and associated vesicular trafficking mechanisms to mediate cellular lipid homeostasisGerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Arterioscler Thromb Vasc Biol 29:1718-22. 2009..This review summarizes the current knowledge of the molecular mechanisms of HDL and associated vesicular trafficking mechanisms to mediate cellular lipid homeostasis...
A bidirectional promoter connects the p14.5 gene to the gene for RNase P and RNase MRP protein subunit hPOP1G Schmiedeknecht
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, D 93042, Germany
Biochem Biophys Res Commun 241:59-67. 1997..These data indicate that the 102 bp region between p14.5 and hPOP1 can act as a bidirectional promoter. The p14.5-hPOP1-cluster was mapped to chromosome 8q22 using in situ hybridization technique...
Analytical capillary isotachophoresis of human serum lipoproteinsG Schmitz
Institut fur Klinische Chemie und Laboratoriumsmedizin, Universitat Regensburg, Germany
Electrophoresis 18:1807-13. 1997..With these characteristics analytical capillary isotachophoresis may be a helpful tool for a fast and reliable automated quantitation of lipoprotein subpopulations in the clinical laboratory...
Scavenging, signalling and adhesion coupling in macrophages: implications for atherogenesisG Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Curr Opin Lipidol 8:287-300. 1997....
Are circulating monocytes as microglia orthologues appropriate biomarker targets for neuronal diseases?Gerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University Hospital of Regensburg, Germany
Cent Nerv Syst Agents Med Chem 9:307-30. 2009..They are suitable biomarker targets for diagnosis and prognosis and maybe also therapy of central nervous system disease...
Update on lipid membrane microdomainsGerd Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Curr Opin Clin Nutr Metab Care 11:106-12. 2008..This review provides an update of membrane microdomain abnormalities...
ATP-binding cassette transporter A1 (ABCA1) in macrophages: a dual function in inflammation and lipid metabolism?G Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Pathobiology 67:236-40. 1999..Our results indicate a dual regulatory function for ABCA1 in macrophage lipid metabolism and inflammation...
Complete coding sequence, promoter region, and genomic structure of the human ABCA2 gene and evidence for sterol-dependent regulation in macrophagesW E Kaminski
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, 93042, Germany
Biochem Biophys Res Commun 281:249-58. 2001..Our results suggest a potential role for ABCA2 in macrophage lipid metabolism and neural development...
ABCG1 (ABC8), the human homolog of the Drosophila white gene, is a regulator of macrophage cholesterol and phospholipid transportJ Klucken
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93042 Regensburg, Germany
Proc Natl Acad Sci U S A 97:817-22. 2000..Our results demonstrating a regulator function for ABCG1 in cholesterol and phospholipid transport define a biologic activity for ABC transporters in macrophages...
ABCA6, a novel a subclass ABC transporterW E Kaminski
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, 93042, Germany
Biochem Biophys Res Commun 285:1295-301. 2001..2. Based on its structural features and its cholesterol-responsive regulation ABCA6 is potentially involved in macrophage lipid homeostasis...
Identification of regulatory elements in the human adipose most abundant gene transcript-1 ( apM-1) promoter: role of SP1/SP3 and TNF-alpha as regulatory pathwaysN Barth
Department of Internal Medicine I, University of Regensburg, 93042 Regensburg, Germany
Diabetologia 45:1425-33. 2002..Our aim was to characterize the tissue-specific regulation of the recently cloned apM-1 promoter and the mechanisms of TNF-alpha-induced downregulation of the apM-1 gene...
Structural organization and characterization of the promoter region of a human carboxylesterase geneT Langmann
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Biochim Biophys Acta 1350:65-74. 1997....
Molecular cloning of the human ATP-binding cassette transporter 1 (hABC1): evidence for sterol-dependent regulation in macrophagesT Langmann
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, 93042, Germany
Biochem Biophys Res Commun 257:29-33. 1999..Our data, demonstrating sterol-dependent regulation of hABC1 in human monocytes/macrophages, suggest a novel role for this transporter molecule in membrane lipid transport...
Regulation of scavenger receptor CD163 expression in human monocytes and macrophages by pro- and antiinflammatory stimuliC Buechler
Institut fur Klinische Chemie und Laboratoriumsmedizin, Klinikum der Universitat Regensburg, Germany
J Leukoc Biol 67:97-103. 2000..This expression pattern implies a functional role of CD 163 in the antiinflammatory response of monocytes...
Mutation analysis of the human adipocyte-specific apM-1 geneA Schaffler
Department of Internal Medicine I, Institute of Clinical Chemistry, University of Regensburg, Germany
Eur J Clin Invest 30:879-87. 2000..The aim of this study was to analyse the human adipocyte-specific apM-1 gene for sequence variations...
Transport of lipids from golgi to plasma membrane is defective in tangier disease patients and Abc1-deficient miceE Orso
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Nat Genet 24:192-6. 2000....
Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in raftsA Pfeiffer
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Eur J Immunol 31:3153-64. 2001..The different receptor complexes may be linked to ligand-specific cellular responses initiated by CD14...
Enzymatically degraded LDL preferentially binds to CD14(high) CD16(+) monocytes and induces foam cell formation mediated only in part by the class B scavenger-receptor CD36M Kapinsky
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Arterioscler Thromb Vasc Biol 21:1004-10. 2001....
Genetic and biochemical evidence that CESD and Wolman disease are distinguished by residual lysosomal acid lipase activityC Aslanidis
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, 93042, Germany
Genomics 33:85-93. 1996....
Pro12Ala polymorphism in the peroxisome proliferator-activated receptor-gamma2 (PPARgamma2) is associated with higher levels of total cholesterol and LDL-cholesterol in male caucasian type 2 diabetes patientsB Zietz
Department of Internal Medicine I, University of Regensburg, D 93042 Regensburg, Germany
Exp Clin Endocrinol Diabetes 110:60-6. 2002..Pro12Ala polymorphism of PPAR(gamma2) gene is not associated with diabetic retinopathy but is associated with dyslipidemia in male type 2 diabetic patients...
The zinc finger protein 202 (ZNF202) is a transcriptional repressor of ATP binding cassette transporter A1 (ABCA1) and ABCG1 gene expression and a modulator of cellular lipid effluxM Porsch-Ozcurumez
Institute for Clinical Chemistry, University of Regensburg, Germany
J Biol Chem 276:12427-33. 2001..7 cells stably expressing ZNF202m1. In conclusion, we have identified ABCA1 and ABCG1 as target genes for ZNF202-mediated repression and thus, provide evidence for a functional linkage between ZNF202 and hypoalphalipoproteinemia...
Genomic organization, promoter cloning, and chromosomal localization of the Dif-2 geneA Pietzsch
Institute for Clinical Chemistry, University of Regensburg, Germany
Biochem Biophys Res Commun 245:651-7. 1998..The Dif-2 gene was mapped to chromosome 6p21.3 using in situ hybridization technique...
Gly15Gly polymorphism within the human adipocyte-specific apM-1gene but not Tyr111His polymorphism is associated with higher levels of cholesterol and LDL-cholesterol in caucasian patients with type 2 diabetesB Zietz
Department of Internal Medicine I, University of Regensburg, D 93042 Regensburg, Germany
Exp Clin Endocrinol Diabetes 109:320-5. 2001..25.0% (TT); p = 0.010). No differences in serum levels of lipids were found in genotype-subgroups of the Tyr111His polymorphism. Thus, Gly15Gly polymorphism of apM-1 gene might play a role in dyslipidaemia in type 2 diabetic patients...
Identification of a novel human sterol-sensitive ATP-binding cassette transporter (ABCA7)W E Kaminski
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg, D 93042, Germany
Biochem Biophys Res Commun 273:532-8. 2000..In macrophages, both the ABCA7 mRNA and protein expression are upregulated in the presence of modified low density lipoprotein and downregulated by HDL(3). Our results suggest a role for ABCA7 in macrophage transmembrane lipid transport...
Gene variants of the bactericidal/permeability increasing protein and lipopolysaccharide binding protein in sepsis patients: gender-specific genetic predisposition to sepsisJ A Hubacek
Institute for Clinical Chemistry and Laboratory Medicine, The University of Regensburg, Germany
Crit Care Med 29:557-61. 2001....
Adipophilin is a sensitive marker for lipid loading in human blood monocytesC Buechler
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053, Regensburg, Germany
Biochim Biophys Acta 1532:97-104. 2001....
The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH)A Schaffler
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Biochem Biophys Res Commun 260:416-25. 1999..Exon- and intron-specific primer sequences are presented as a basis for mutation screening of patients affected with FCH...
Genomic organization and characterization of the promoter of the human ATP-binding cassette transporter-G1 (ABCG1) geneT Langmann
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, 93042, Regensburg, Germany
Biochim Biophys Acta 1494:175-80. 2000..The TATA-less proximal promoter contains multiple Sp1 binding sites and a consensus sequence for sterol regulatory element binding protein...
Altered mononuclear phagocyte differentiation associated with genetic defects of the lysosomal acid lipaseG Rothe
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Atherosclerosis 130:215-21. 1997..In conclusion, our data suggest that these monogenetic defects of lysosomal lipoprotein metabolism are associated with complex alterations of mononuclear phagocyte differentiation and extravasation...
Identification and characterization of a novel monocyte/macrophage differentiation-dependent gene that is responsive to lipopolysaccharide, ceramide, and lysophosphatidylcholineA Pietzsch
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Biochem Biophys Res Commun 235:4-9. 1997..Our results indicate that DIF-2 represents a gene which is regulated in differentiation processes and strongly responsive to lipopolysaccharide, ceramide and lysophosphatidylcholine...
ATP-binding cassette transporter A1 (ABCA1) affects total body sterol metabolismW Drobnik
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, D 93042 Regensburg, Germany
Gastroenterology 120:1203-11. 2001..Our aim was to further analyze the role of ABCA1 in cholesterol metabolism...
Nitrous oxide impairs the neutrophil oxidative responseD Frohlich
Department of Anesthesiology, University of Regensburg, Germany
Anesthesiology 88:1281-90. 1998..The choice of the stimulus, receptor agonists, or stimuli acting independent of receptors seems to determine whether nitrous oxide impairs the oxidative functions, suggesting an interference with the cytosolic signaling of neutrophils...
Post-hoc analysis on the CD14 C(-260)T promoter polymorphism and coronary heart diseaseM Porsch-Ozcurumez
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Physiol Res 56:727-33. 2007..Due to the small sample size and post-hoc character of the study large-scale prospective studies that monitor patients with proven CHD are needed to confirm these findings...
Acyl-CoA binding protein (ACBP) regulates acyl-CoA:cholesterol acyltransferase (ACAT) in human mononuclear phagocytesC Kerkhoff
Institut fur Klinische Chemie und Laboratoriumsmedizin, Klinikum der Universitat Regensburg, Germany
Biochim Biophys Acta 1346:163-72. 1997..This common binding site is suggested to be responsible for the transfer from ACBP-bound oleoyl-CoA to ACAT and, therefore, to be essential for the microsomal cholesterol esterification...
Rapid quantification of human ABCA1 mRNA in various cell types and tissues by real-time reverse transcription-PCRD Kielar
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93042 Regensburg, Germany
Clin Chem 47:2089-97. 2001..We developed a rapid, sensitive, specific, and reproducible real-time reverse transcription-PCR (RT-PCR) method for detection and quantification of ABCA1 transcripts in total RNA isolated from cultured human cells and tissues...
Apo AI/ABCA1-dependent and HDL3-mediated lipid efflux from compositionally distinct cholesterol-based microdomainsWolfgang Drobnik
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Traffic 3:268-78. 2002..In summary, we provide evidence for the existence of compositionally distinct membrane microdomains in human cells and their modulation by apo AI/ABCA1-dependent and HDL3-mediated lipid efflux...
Beta-amyloid (Abeta40, Abeta42) binding to modified LDL accelerates macrophage foam cell formationBerta Schulz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93042 Regensburg, Germany
Biochim Biophys Acta 1771:1335-44. 2007..In summary, our results suggest that Abeta42 and Abeta40 act as potent opsonins for LDL, E-LDL and Ox-LDL and enhance cellular cholesterol accumulation as well as Abeta-deposition in vessel wall macrophages...
HIF-1beta determines ABCA1 expression under hypoxia in human macrophagesPeter Ugocsai
Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Franz Josef Strauss Allee 11, Regensburg, Germany
Int J Biochem Cell Biol 42:241-52. 2010..These results demonstrate that HIF-1beta availability determines ABCA1 expression and cholesterol efflux in macrophages under hypoxia and may contribute to the interpersonal variability of atherosclerotic lesion progression...
ABCA10, a novel cholesterol-regulated ABCA6-like ABC transporterJürgen J Wenzel
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, Regensburg D 93042, Germany
Biochem Biophys Res Commun 306:1089-98. 2003..Our results identify ABCA10 as a novel member of the group of ABCA6-like transporters and suggest its involvement in macrophage lipid homeostasis...
Ezetimib influences the expression of raft-associated antigens in human monocytesEvelyn Orso
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Cytometry A 69:206-8. 2006..Ezetimib significantly decreased the surface expression of CD13, CD16, CD64, and CD36; furthermore, it induced a shift of CD13 from plasma membrane to intracellular vesicles, and thus it quite likely modulated monocytic raft-assembly...
Genomic organization and chromosomal localization of the human CD163 (M130) gene: a member of the scavenger receptor cysteine-rich superfamilyM Ritter
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, D 93042, Germany
Biochem Biophys Res Commun 260:466-74. 1999..In addition, we identified a L1 element located 1.4 kb upstream of the major transcription initiation site...
Loss of detoxification in inflammatory bowel disease: dysregulation of pregnane X receptor target genesThomas Langmann
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Gastroenterology 127:26-40. 2004..Thus, dysregulation of xenobiotic metabolism and PXR activity in the gut is likely to contribute to the pathophysiology of UC...
Changes in HDL-associated apolipoproteins relate to mortality in human sepsis and correlate to monocyte and platelet activationStefan Barlage
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93042, Regensburg, Germany
Intensive Care Med 35:1877-85. 2009..In this study, we analyzed whether plasma concentrations of lipoproteins and apolipoproteins in human sepsis are related to patient survival and the activation of blood monocytes and platelets...
Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophagesMargot Grandl
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Cytometry A 77:231-42. 2010..Therefore, fluorescent high-content imaging is a useful tool to discriminate between and quantify lipid storage compartments in macrophages also in response to drugs affecting cellular lipid metabolism...
Monocyte cholesterol homeostasis correlates with the presence of detergent resistant membrane microdomainsZsuzsanna Wolf
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Cytometry A 71:486-94. 2007..Lipid membrane microdomains are involved in the regulation of biological functions of monocyte membrane proteins. These microdomains show a relative resistance to non-ionic detergents providing an easy analytical tool to study them...
Isolation and characterization of a 14.5-kDa trichloroacetic-acid-soluble translational inhibitor protein from human monocytes that is upregulated upon cellular differentiationG Schmiedeknecht
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Eur J Biochem 242:339-51. 1996..g. a differentiation-dependent regulation of protein synthesis in hepatocytes, renal tubular epithelial cells, smooth muscle cells and MNP...
Interaction of CD163 with the regulatory subunit of casein kinase II (CKII) and dependence of CD163 signaling on CKII and protein kinase CM Ritter
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Eur J Immunol 31:999-1009. 2001..Inhibition studies using specific kinase inhibitors reveal that both CKII and PKC are involved in the CD163 signaling mechanism resulting in the secretion of proinflammatory cytokines...
Expression of the lysophospholipid receptor family and investigation of lysophospholipid-mediated responses in human macrophagesChinh Quoc Duong
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93042, Regensburg, Germany
Biochim Biophys Acta 1682:112-9. 2004..Altogether, these data give insights into the expression and regulation of lysophospholipid receptors in primary human monocytes, macrophages and foam cells...
High density lipoprotein modulates platelet functionStefan Barlage
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Cytometry A 69:196-9. 2006..CONCLUSION: Our results suggest that HDL modulates platelet reactivity by altering lipid raft associated receptor clustering...
Homozygosity for the 168His variant of the minor histocompatibility antigen HA-1 is associated with reduced risk of primary Sjögren's syndromeMariann Harangi
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, D 93042 Regensburg, Germany
Eur J Immunol 35:305-17. 2005..Our results suggest that the HA-1 168His variant is associated with reduced susceptibility to primary Sjögren's syndrome...
Gene expression profiling identifies retinoids as potent inducers of macrophage lipid effluxThomas Langmann
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93042 Regensburg, Germany
Biochim Biophys Acta 1740:155-61. 2005..We observed a strong induction of apoA-I-dependent lipid efflux in stimulated macrophages, implicating an important role for retinoids in cellular functions of macrophages...
Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cellsSalim Maa Bared
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93040 Regensburg, Germany
Mol Biol Cell 15:5399-407. 2004..It is concluded that ABCA1 forms a complex with syntaxin 13 and flotillin-1, residing at the plasma membrane and in phagosomes that are partially located in raft microdomains...
Molecular and functional interaction of the ATP-binding cassette transporter A1 with Fas-associated death domain proteinChrista Buechler
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
J Biol Chem 277:41307-10. 2002..The association of FADD with ABCA1 provides an unexpected link between high density lipoprotein metabolism and an adaptor molecule mainly described in death receptor signal transduction...
Novel sphingolipid derivatives promote keratinocyte differentiationGyorgy Paragh
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
Exp Dermatol 17:1004-16. 2008..Taken together, these results demonstrate the gene regulatory potential of sphingolipid species that could be valuable for dermatological or cosmetic applications...
Evaluation of a high-content screening fluorescence-based assay analyzing the pharmacological modulation of lipid homeostasis in human macrophagesTobias Werner
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Cytometry A 69:200-2. 2006....
Definition and characterization of relative hypo- and hyperleptinemia in a large Caucasian populationR Buettner
Department of Internal Medicine I, University of Regensburg, 93042 Regensburg, Germany
J Endocrinol 175:745-56. 2002..In conclusion, we report the first percentile curves for serum leptin by BMI in a large Caucasian population. Relatively low leptin values may be associated with a lower metabolic risk than relatively high serum leptin values...
Alterations in intestinal fatty acid metabolism in inflammatory bowel diseaseSusanne Heimerl
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93042 Regensburg, Germany
Biochim Biophys Acta 1762:341-50. 2006..Observed alterations in expression of genes of fatty acid metabolism may contribute to the pathophysiology of ulcerative colitis...
Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cellsSusanne Heimerl
Institute of Clinical Chemistry, University of Regensburg, Regensburg, Germany
Melanoma Res 17:265-73. 2007....
Mass spectrometric analysis of lipid species of human circulating blood cellsKatharina Leidl
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Biochim Biophys Acta 1781:655-64. 2008..This work could be a reference for studies in different patient cohorts directed towards discovery of novel lipid biomarkers in circulating blood cells...
Expression of tumor necrosis factor alpha and its receptors during cellular differentiationPetra Schling
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, 93053 Regensburg, Germany
Cytokine 33:239-45. 2006..Non-signaling TNF-receptors may protect chondrocytes and osteocytes from the anti-differentiation effects of local TNFalpha production...
Lipoprotein (a) downregulates lysosomal acid lipase and induces interleukin-6 in human blood monocytesChrista Buechler
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Straubeta Allee 11, D-93042 Regensburg, Germany
Biochim Biophys Acta 1642:25-31. 2003..In addition, increased levels of IL-6 also predict future cardiovascular events and IL-6 secretion was also induced by purified Lp(a)...
The carboxyterminus of the ATP-binding cassette transporter A1 interacts with a beta2-syntrophin/utrophin complexChrista Buechler
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, D 93053 Regensburg, Germany
Biochem Biophys Res Commun 293:759-65. 2002....
Molecular structure of a novel cholesterol-responsive A subclass ABC transporter, ABCA9Armin Piehler
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, D 93042, Regensburg, Germany
Biochem Biophys Res Commun 295:408-16. 2002..Based on this information, ABCA9 is likely involved in monocyte differentiation and macrophage lipid homeostasis...
Induction of fatty acid synthesis is a key requirement for phagocytic differentiation of human monocytesJosef Ecker
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93042 Regensburg, Germany
Proc Natl Acad Sci U S A 107:7817-22. 2010..Therefore induction of fatty acid synthesis is a key requirement for phagocyte development and function...
Effect of HMG-CoA-reductase inhibitors on survival in type 2 diabetes patients with end stage diabetic nephropathyA K Götz
Klinik und Poliklinik fur Innere Medizin II, University of Regensburg, Franz Josef Strauss Allee 11, D 93053 Regensburg, Germany
Eur J Med Res 10:155-60. 2005..Since the efficacy of CSE-I in dialysis patients is discussed controversially, we tested the hypothesis that only patients with LDL-cholesterol > 100 mg/dl benefit from CSE-I...
Identification and characterization of the human adipocyte apM-1 promoterA Schaffler
Institute for Clinical Chemistry and Laboratory Medicine, University Clinic of Regensburg, Germany
Biochim Biophys Acta 1399:187-97. 1998..In addition, an exon/intron boundary was mapped at the extreme 5'-end of the cDNA sequence. Genomic Southern blotting suggests that the human apM-1 gene is a single copy gene...
The genomic organization of the genes for human lipopolysaccharide binding protein (LBP) and bactericidal permeability increasing protein (BPI) is highly conservedJ A Hubacek
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
Biochem Biophys Res Commun 236:427-30. 1997..Our results indicate that the LBP, BPI, and PLTP genes, and probably the CETP gene, may have evolved from a common primordial gene and may share similar functional properties...
Influence of interleukin-1 gene polymorphism on periodontal regeneration in intrabony defectsM Christgau
Department of Operative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany
J Periodontal Res 38:20-7. 2003..In conclusion, these 12-month findings indicate that the IL-1 gene polymorphism has no influence on the clinical and radiographic regeneration results following GTR therapy...
Nitrous oxide impairs the signaling of neutrophils downstream of receptorsD Frohlich
Department of Anaesthesia, University of Regensburg, Germany
Toxicol Lett 100:121-7. 1998..4) No interference was found at the receptor sites, the linked G proteins, and the subsequent release of Ca2+, indicating a localization of the nitrous oxide interaction downstream of receptors and G proteins at or near to PKC...
