Gerhart U Ryffel

Summary

Affiliation: University of Essen
Country: Germany

Publications

  1. ncbi request reprint Mutations in the human genes encoding the transcription factors of the hepatocyte nuclear factor (HNF)1 and HNF4 families: functional and pathological consequences
    G U Ryffel
    Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122 Essen, Germany
    J Mol Endocrinol 27:11-29. 2001
  2. pmc Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus
    Gerhart U Ryffel
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Hufelandstrasse 55, D 45122 Essen, Germany
    Nucleic Acids Res 31:e44. 2003
  3. ncbi request reprint What can a frog tell us about human kidney development
    Gerhart U Ryffel
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Essen, Germany
    Nephron Exp Nephrol 94:e35-43. 2003
  4. ncbi request reprint Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus
    G U Ryffel
    Institut für Zellbiologie Tumorforschung Universitätsklinikum Essen, Hufelandstrasse 55, D 45122, Essen, Germany
    Mech Dev 90:65-75. 2000
  5. ncbi request reprint Distinct molecular and morphogenetic properties of mutations in the human HNF1beta gene that lead to defective kidney development
    Silvia Bohn
    Institut fur Zellbiologie, Universitatsklinikum Essen, Essen, Germany
    J Am Soc Nephrol 14:2033-41. 2003
  6. ncbi request reprint Tissue-specific transcription factor HNF4alpha inhibits cell proliferation and induces apoptosis in the pancreatic INS-1 beta-cell line
    Silke Erdmann
    Universitatsklinikum Essen, Institut fur Zellbiologie, D 45122 Essen, Germany
    Biol Chem 388:91-106. 2007
  7. doi request reprint Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis
    Magdalena Roose
    Institut für Zellbiologie Tumorforschung, Universitat Duisburg Essen, Hufelandstr 55, 45122, Essen, Germany
    Transgenic Res 18:595-605. 2009
  8. ncbi request reprint Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line
    Sabine Senkel
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, D 45122 Essen, Germany
    Biochim Biophys Acta 1731:179-90. 2005
  9. ncbi request reprint The HNF1beta transcription factor has several domains involved in nephrogenesis and partially rescues Pax8/lim1-induced kidney malformations
    Guizhi Wu
    Institut fur Zellbiologie, Universitatsklinikum Essen, Germany
    Eur J Biochem 271:3715-28. 2004
  10. doi request reprint Transcription factor HNF1beta and novel partners affect nephrogenesis
    Karin Dudziak
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Essen, Germany
    Kidney Int 74:210-7. 2008

Collaborators

  • Christoph Waldner
  • Coralie Bingham
  • Andrew T Hattersley
  • Heike Weber
  • Kazuhiro Sakamaki
  • Naoto Ueno
  • Bernhard Horsthemke
  • S Ellard
  • Timothy M Frayling
  • Sabine Senkel
  • Ludger Klein-Hitpass
  • Heike Thomas
  • Belén Lucas
  • Magdalena Roose
  • Kathrin Sauert
  • Silke Erdmann
  • Andrea Wirsing
  • S Senkel
  • Karen Grigo
  • Gülüzar Turan
  • Jörn Lausen
  • Christiane Drews
  • Anna Kosubek
  • Silvia Bohn
  • Karin Dudziak
  • B Holewa
  • E Pogge v Strandmann
  • Tanja Arndt
  • Guizhi Wu
  • E P Strandmann
  • André W Brändli
  • S Bogdan
  • H Thomas
  • E Pogge von Strandmann
  • Mazhar Gull
  • Stefan Kahnert
  • D Werdien
  • D Zapp
  • Katrin Rademacher
  • Natalie Solomentsew
  • Kallal Pramanik
  • T Drewes
  • Dagmar Werdien
  • Stefan Rosengarten
  • Nima Mottalebi
  • Emma L Edghill
  • H Nakhei
  • Ira Lemm
  • Michael Bulman
  • Britta Badenberg
  • Simone Roosen
  • Lucy Kind
  • S M Mitchell
  • M Bulman
  • C J Tack
  • K Jaschkowitz
  • F Esser
  • G Peiler
  • S Roosen
  • A Lingott-Frieg
  • E Pogge yon Strandmann
  • S Bartkowski
  • I Lemm
  • A Lingott
  • C Zoidl
  • P Lorenz
  • L Klein-Hitpass
  • G Eberle

Detail Information

Publications38

  1. ncbi request reprint Mutations in the human genes encoding the transcription factors of the hepatocyte nuclear factor (HNF)1 and HNF4 families: functional and pathological consequences
    G U Ryffel
    Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122 Essen, Germany
    J Mol Endocrinol 27:11-29. 2001
    ..This review summarizes the main features of these transcription factors and discusses potential events leading to the specific disease phenotypes...
  2. pmc Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus
    Gerhart U Ryffel
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Hufelandstrasse 55, D 45122 Essen, Germany
    Nucleic Acids Res 31:e44. 2003
    ..We detect a dramatic increase in transgene expression at the site of tissue removal in the tail stump. In the regenerated tail, yellow fluorescence is restricted to the myotomes thus excluding transdifferentiation of muscle cells...
  3. ncbi request reprint What can a frog tell us about human kidney development
    Gerhart U Ryffel
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Essen, Germany
    Nephron Exp Nephrol 94:e35-43. 2003
    ..Thus, Xenopus pronephros formation can be used to define nephrogenic regulators and to identify the morphogenetic potential of mutated factors associated with renal diseases in humans...
  4. ncbi request reprint Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus
    G U Ryffel
    Institut für Zellbiologie Tumorforschung Universitätsklinikum Essen, Hufelandstrasse 55, D 45122, Essen, Germany
    Mech Dev 90:65-75. 2000
    ..In conclusion, our data establish that the generation of transgenic Xenopus allows the functional dissection of promoters in the context of the entire organism...
  5. ncbi request reprint Distinct molecular and morphogenetic properties of mutations in the human HNF1beta gene that lead to defective kidney development
    Silvia Bohn
    Institut fur Zellbiologie, Universitatsklinikum Essen, Essen, Germany
    J Am Soc Nephrol 14:2033-41. 2003
    ..The distinct properties observed in the various HNF1beta mutants may guide the classification of the phenotypes observed in patients with a mutated HNF1beta gene...
  6. ncbi request reprint Tissue-specific transcription factor HNF4alpha inhibits cell proliferation and induces apoptosis in the pancreatic INS-1 beta-cell line
    Silke Erdmann
    Universitatsklinikum Essen, Institut fur Zellbiologie, D 45122 Essen, Germany
    Biol Chem 388:91-106. 2007
    ..We propose that the anti-proliferative and apoptotic properties of HNF4alpha may be an essential feature impaired in MODY1 and possibly also in type 2 diabetes...
  7. doi request reprint Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis
    Magdalena Roose
    Institut für Zellbiologie Tumorforschung, Universitat Duisburg Essen, Hufelandstr 55, 45122, Essen, Germany
    Transgenic Res 18:595-605. 2009
    ..Thus, we have established the first binary inducible gene expression system in Xenopus laevis that can be used to study organogenesis...
  8. ncbi request reprint Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line
    Sabine Senkel
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, D 45122 Essen, Germany
    Biochim Biophys Acta 1731:179-90. 2005
    ..Since nine of the potential HNF1beta target genes are deregulated in clear cell carcinoma of the ovary, we propose that HNF1beta overexpression in the ovarian cancer participates in the altered expression pattern...
  9. ncbi request reprint The HNF1beta transcription factor has several domains involved in nephrogenesis and partially rescues Pax8/lim1-induced kidney malformations
    Guizhi Wu
    Institut fur Zellbiologie, Universitatsklinikum Essen, Germany
    Eur J Biochem 271:3715-28. 2004
    ..We conclude that HNF1beta has some distinct morphogenetic properties during nephrogenesis...
  10. doi request reprint Transcription factor HNF1beta and novel partners affect nephrogenesis
    Karin Dudziak
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Essen, Germany
    Kidney Int 74:210-7. 2008
    ..We searched for mutations in the open reading frame of the ZFP36L1 gene in 58 patients with renal anomalies but found none...
  11. ncbi request reprint HNF4alpha reduces proliferation of kidney cells and affects genes deregulated in renal cell carcinoma
    Belén Lucas
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, D 45122 Essen, Germany
    Oncogene 24:6418-31. 2005
    ..These genes (ACY1, WT1, SELENBP1, COBL, EFHD1, AGXT2L1, ALDH5A1, THEM2, ABCB1, FLJ14146, CSPG2, TRIM9 and HEY1) are good candidates for genes whose activity is changed upon the decrease of HNF4alpha in RCC...
  12. doi request reprint HNF4 alpha orchestrates a set of 14 genes to down-regulate cell proliferation in kidney cells
    Karen Grigo
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, D 45122 Essen, Germany
    Biol Chem 389:179-87. 2008
    ..Therefore, we postulate that HNF4 alpha orchestrates, at least, these 14 genes to regulate cell proliferation in HEK293 cells and that down-regulation of HNF4 alpha could contribute to the progression of kidney cancer...
  13. pmc Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line
    Heike Thomas
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, D 45122 Essen, Germany
    Nucleic Acids Res 32:e150. 2004
    ..We show that the expression of the mutant P328L329del and A263insGG affects only very few transcripts and these are predominantly distinct from those induced by wild-type HNF1beta...
  14. ncbi request reprint Developmental expression of the maternal protein XDCoH, the dimerization cofactor of the homeoprotein LFB1 (HNF1)
    E Pogge yon Strandmann
    Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Germany
    Development 121:1217-26. 1995
    ..It seems likely that XDCoH interaction is not only essential for the function of LFB1 and LFB3 but also for certain other transcription factors...
  15. ncbi request reprint Transcriptional hierarchy in Xenopus embryogenesis: HNF4 a maternal factor involved in the developmental activation of the gene encoding the tissue specific transcription factor HNF1 alpha (LFB1)
    B Holewa
    Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Germany
    Mech Dev 54:45-57. 1996
    ....
  16. pmc Heat-shock mediated overexpression of HNF1β mutations has differential effects on gene expression in the Xenopus pronephric kidney
    Kathrin Sauert
    Institut fur Zellbiologie, Tumorforschung, Universitatsklinikum Essen, Essen, Germany
    PLoS ONE 7:e33522. 2012
    ....
  17. ncbi request reprint Transgenic Xenopus laevis strain expressing cre recombinase in muscle cells
    Christoph Waldner
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Essen, Germany
    Dev Dyn 235:2220-8. 2006
    ..This first Cre-expressing strain allows conditional activation of any gene of interest in muscle cells and, thus, opens up the use of recombinases as a new experimental strategy in Xenopus...
  18. pmc A systematic analysis of the 3'UTR of HNF4A mRNA reveals an interplay of regulatory elements including miRNA target sites
    Andrea Wirsing
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Essen, Germany
    PLoS ONE 6:e27438. 2011
    ..The identification of several regulatory elements within the HNF4A 3'UTR justifies the analysis of the 3'UTR sequence to explore the dysfunction of HNF4α in diabetes and RCC...
  19. ncbi request reprint Regulation and function of the tissue-specific transcription factor HNF1 alpha (LFB1) during Xenopus development
    H Weber
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum, Essen, Germany
    Int J Dev Biol 40:297-304. 1996
    ..As the HNF1 alpha promoter also contains functional binding sites for HNF4 and HNF1, we postulate that all of these transcription factors contribute to the cascade leading to proper embryonic activation of the HNF1 alpha gene...
  20. pmc HNF4beta, a new gene of the HNF4 family with distinct activation and expression profiles in oogenesis and embryogenesis of Xenopus laevis
    B Holewa
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Germany
    Mol Cell Biol 17:687-94. 1997
    ..All of these distinct characteristics of the newly identified HNF4 protein imply that the alpha and beta isoform have different functions in development and in adult tissues...
  21. pmc Red fluorescent Xenopus laevis: a new tool for grafting analysis
    Christoph Waldner
    Institut für Zellbiologie Tumorforschung, Universitat Duisburg Essen, D 45122 Essen, Germany
    BMC Dev Biol 9:37. 2009
    ..To track more than one event in the same animal, fluorescent markers that clearly differ in their emission spectrum are needed...
  22. pmc The nephrogenic potential of the transcription factors osr1, osr2, hnf1b, lhx1 and pax8 assessed in Xenopus animal caps
    Christiane Drews
    Institut für Zellbiologie Tumorforschung Universitätsklinikum Essen, Universitat Duisburg Essen, Hufelandstrasse 55, 45122 Essen, Germany
    BMC Dev Biol 11:5. 2011
    ..Treatment of the undifferentiated animal pole explant (animal cap) of Xenopus with activin A and retinoic acid induces pronephros formation providing a powerful tool to analyze key molecular events in nephrogenesis...
  23. pmc Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis
    S Bartkowski
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Germany
    Mol Cell Biol 13:421-31. 1993
    ..The early appearance of XLFB1 expression during embryogenesis suggests that the tissue-specific transcription factor XLFB1 is involved in the determination and/or differentiation of specific cell types during organogenesis...
  24. ncbi request reprint A distant upstream promoter of the HNF-4alpha gene connects the transcription factors involved in maturity-onset diabetes of the young
    H Thomas
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Hufelandstrasse 55, D 45122 Essen, Germany
    Hum Mol Genet 10:2089-97. 2001
    ..25). These data suggest a regulatory network of the four MODY transcription factors interconnected at the distant upstream P2 promoter of the HNF-4alpha gene...
  25. pmc An alternative splice variant of the tissue specific transcription factor HNF4alpha predominates in undifferentiated murine cell types
    H Nakhei
    Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122 Essen, Germany
    Nucleic Acids Res 26:497-504. 1998
    ..All these features suggest that the presence of distinct splice variants of HNF4alpha modulates the activity of HNF4alphain a cell type specific way...
  26. ncbi request reprint The bifunctional protein DCoH/PCD, a transcription factor with a cytoplasmic enzymatic activity, is a maternal factor in the rat egg and expressed tissue specifically during embryogenesis
    E P Strandmann
    Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Germany
    Int J Dev Biol 42:53-9. 1998
    ....
  27. ncbi request reprint Ectopic pigmentation in Xenopus in response to DCoH/PCD, the cofactor of HNF1 transcription factor/pterin-4alpha-carbinolamine dehydratase
    E Pogge v Strandmann
    Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122, Essen, Germany
    Mech Dev 91:53-60. 2000
    ....
  28. pmc Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes
    T Drewes
    Institut für Zelbiologie, Universitatsklinikum Essen, Germany
    Mol Cell Biol 16:925-31. 1996
    ..Therefore, the differential expression of distinct HNF4 proteins may play a key role in the differential transcriptional regulation of HNF4-dependent genes...
  29. ncbi request reprint Misexpression of Xsiah-2 induces a small eye phenotype in Xenopus
    S Bogdan
    Institute of Cell Biology, University of Essen, Hufelandstrasse 55, D 45122 Essen, Germany
    Mech Dev 103:61-9. 2001
    ..As this phenotype has been also described for flies expressing sina ectopically, the data demonstrate the functional and structural conservation of Xsiah-2 and sina in metazoan eye development...
  30. ncbi request reprint Genomic structure of the Xenopus laevis liver transcription factor LFB1
    D Zapp
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Germany
    Gene 134:251-6. 1993
    ..This is in agreement with the structure found in the rat gene and supports the notion that the LFB1 homeobox evolved separately from the other genes encoding homeodomain proteins...
  31. ncbi request reprint ERH (enhancer of rudimentary homologue), a conserved factor identical between frog and human, is a transcriptional repressor
    E Pogge von Strandmann
    , , Germany
    Biol Chem 382:1379-85. 2001
    ..Furthermore, the DCoH/PCD coactivation of a HNF1 responsive reporter is inhibited by XERH. We propose that XERH is a cell type-specific transcriptional repressor, probably interfering with HNF1-dependent gene regulation via DCoH/PCD...
  32. pmc Improved conditional expression systems resulting in physiological level of HNF4alpha expression confirm HNF4alpha induced apoptosis in the pancreatic beta-cell line INS-1
    Sabine Senkel
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat, Duisburg Essen, D 45122 Essen, Germany
    BMC Res Notes 2:210. 2009
    ..However, leakiness of basal transgene expression and artificially high expression level upon tetracycline addition may be disadvantageous...
  33. ncbi request reprint Marking transgenic Xenopus froglets with passive micro transponders
    Christoph Waldner
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Hufelandstrasse 55, Essen, Germany
    Transgenic Res 16:539-40. 2007
    ..This new technique makes the breeding of transgenic frogs most efficient, because the frogs do not have to be kept separately and they grow much faster when kept together in large containers...
  34. ncbi request reprint Evidence for haploinsufficiency of the human HNF1alpha gene revealed by functional characterization of MODY3-associated mutations
    Heike Thomas
    Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122 Essen, Germany
    Biol Chem 383:1691-700. 2002
    ..Our results suggest that haploinsufficiency of HNF1alpha is responsible for the pathogenesis of MODY3...
  35. pmc Aging of Xenopus tropicalis eggs leads to deadenylation of a specific set of maternal mRNAs and loss of developmental potential
    Anna Kosubek
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Essen, Germany
    PLoS ONE 5:e13532. 2010
    ..Based on this hypothesis the developmental consequences of downregulation of specific transcripts can be analyzed in future...
  36. pmc Double conditional human embryonic kidney cell line based on FLP and ΦC31 mediated transgene integration
    Christoph Waldner
    Institut für Zellbiologie Tumorforschung, Universitat Duisburg Essen, Hufelandstr, 55, D 45122 Essen, Germany
    BMC Res Notes 4:420. 2011
    ..abstract:..
  37. doi request reprint Orgenic plants: gene-manipulated plants compatible with organic farming
    Gerhart U Ryffel
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Essen, Germany
    Biotechnol J 7:1328-31. 2012
    ..These GM plants are likely to be acceptable to most skeptics of GM plants and facilitate the use of innovative new crops...
  38. pmc FLP and Cre recombinase function in Xenopus embryos
    D Werdien
    , , Hufelandstrasse 55, D-45122 Essen, Germany
    Nucleic Acids Res 29:E53-3. 2001
    ....