Research Topics
Genomes and GenesSpecies | Gerhart U RyffelSummaryAffiliation: University of Essen Country: Germany Publications
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Publications
What can a frog tell us about human kidney developmentGerhart U Ryffel
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Essen, Germany
Nephron Exp Nephrol 94:e35-43. 2003..Thus, Xenopus pronephros formation can be used to define nephrogenic regulators and to identify the morphogenetic potential of mutated factors associated with renal diseases in humans...
Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic XenopusGerhart U Ryffel
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Hufelandstrasse 55, D 45122 Essen, Germany
Nucleic Acids Res 31:e44. 2003..We detect a dramatic increase in transgene expression at the site of tissue removal in the tail stump. In the regenerated tail, yellow fluorescence is restricted to the myotomes thus excluding transdifferentiation of muscle cells...- Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic XenopusG U Ryffel
Institut für Zellbiologie Tumorforschung Universitätsklinikum Essen, Hufelandstrasse 55, D 45122, Essen, Germany
Mech Dev 90:65-75. 2000..In conclusion, our data establish that the generation of transgenic Xenopus allows the functional dissection of promoters in the context of the entire organism... - Mutations in the human genes encoding the transcription factors of the hepatocyte nuclear factor (HNF)1 and HNF4 families: functional and pathological consequencesG U Ryffel
Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122 Essen, Germany
J Mol Endocrinol 27:11-29. 2001..This review summarizes the main features of these transcription factors and discusses potential events leading to the specific disease phenotypes... - Tissue-specific transcription factor HNF4alpha inhibits cell proliferation and induces apoptosis in the pancreatic INS-1 beta-cell lineSilke Erdmann
Universitatsklinikum Essen, Institut fur Zellbiologie, D 45122 Essen, Germany
Biol Chem 388:91-106. 2007..We propose that the anti-proliferative and apoptotic properties of HNF4alpha may be an essential feature impaired in MODY1 and possibly also in type 2 diabetes... - Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevisMagdalena Roose
Institut für Zellbiologie Tumorforschung, Universitat Duisburg Essen, Hufelandstr 55, 45122, Essen, Germany
Transgenic Res 18:595-605. 2009..Thus, we have established the first binary inducible gene expression system in Xenopus laevis that can be used to study organogenesis... - Distinct molecular and morphogenetic properties of mutations in the human HNF1beta gene that lead to defective kidney developmentSilvia Bohn
, , Essen, Germany
J Am Soc Nephrol 14:2033-41. 2003..The distinct properties observed in the various HNF1beta mutants may guide the classification of the phenotypes observed in patients with a mutated HNF1beta gene... - Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell lineSabine Senkel
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, D 45122 Essen, Germany
Biochim Biophys Acta 1731:179-90. 2005..Since nine of the potential HNF1beta target genes are deregulated in clear cell carcinoma of the ovary, we propose that HNF1beta overexpression in the ovarian cancer participates in the altered expression pattern... - Transcription factor HNF1beta and novel partners affect nephrogenesisKarin Dudziak
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Essen, Germany
Kidney Int 74:210-7. 2008..We searched for mutations in the open reading frame of the ZFP36L1 gene in 58 patients with renal anomalies but found none... - The HNF1beta transcription factor has several domains involved in nephrogenesis and partially rescues Pax8/lim1-induced kidney malformationsGuizhi Wu
Institut fur Zellbiologie, Universitatsklinikum Essen, Germany
Eur J Biochem 271:3715-28. 2004..We conclude that HNF1beta has some distinct morphogenetic properties during nephrogenesis... - HNF4 alpha orchestrates a set of 14 genes to down-regulate cell proliferation in kidney cellsKaren Grigo
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, D 45122 Essen, Germany
Biol Chem 389:179-87. 2008..Therefore, we postulate that HNF4 alpha orchestrates, at least, these 14 genes to regulate cell proliferation in HEK293 cells and that down-regulation of HNF4 alpha could contribute to the progression of kidney cancer... - HNF4alpha reduces proliferation of kidney cells and affects genes deregulated in renal cell carcinomaBelén Lucas
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, D 45122 Essen, Germany
Oncogene 24:6418-31. 2005..These genes (ACY1, WT1, SELENBP1, COBL, EFHD1, AGXT2L1, ALDH5A1, THEM2, ABCB1, FLJ14146, CSPG2, TRIM9 and HEY1) are good candidates for genes whose activity is changed upon the decrease of HNF4alpha in RCC... - Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell lineHeike Thomas
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, D 45122 Essen, Germany
Nucleic Acids Res 32:e150. 2004..We show that the expression of the mutant P328L329del and A263insGG affects only very few transcripts and these are predominantly distinct from those induced by wild-type HNF1beta... - Developmental expression of the maternal protein XDCoH, the dimerization cofactor of the homeoprotein LFB1 (HNF1)E Pogge yon Strandmann
Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Germany
Development 121:1217-26. 1995..It seems likely that XDCoH interaction is not only essential for the function of LFB1 and LFB3 but also for certain other transcription factors... - Transcriptional hierarchy in Xenopus embryogenesis: HNF4 a maternal factor involved in the developmental activation of the gene encoding the tissue specific transcription factor HNF1 alpha (LFB1)B Holewa
Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Germany
Mech Dev 54:45-57. 1996.... - Transgenic Xenopus laevis strain expressing cre recombinase in muscle cellsChristoph Waldner
, , Essen, Germany
Dev Dyn 235:2220-8. 2006..This first Cre-expressing strain allows conditional activation of any gene of interest in muscle cells and, thus, opens up the use of recombinases as a new experimental strategy in Xenopus... - Heat-shock mediated overexpression of HNF1β mutations has differential effects on gene expression in the Xenopus pronephric kidneyKathrin Sauert
Institut fur Zellbiologie, Tumorforschung, Universitatsklinikum Essen, Essen, Germany
PLoS ONE 7:e33522. 2012.... - A systematic analysis of the 3'UTR of HNF4A mRNA reveals an interplay of regulatory elements including miRNA target sitesAndrea Wirsing
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Essen, Germany
PLoS ONE 6:e27438. 2011..The identification of several regulatory elements within the HNF4A 3'UTR justifies the analysis of the 3'UTR sequence to explore the dysfunction of HNF4α in diabetes and RCC... - Regulation and function of the tissue-specific transcription factor HNF1 alpha (LFB1) during Xenopus developmentH Weber
Institut für Zellbiologie Tumorforschung, Universitatsklinikum, Essen, Germany
Int J Dev Biol 40:297-304. 1996..As the HNF1 alpha promoter also contains functional binding sites for HNF4 and HNF1, we postulate that all of these transcription factors contribute to the cascade leading to proper embryonic activation of the HNF1 alpha gene... - HNF4beta, a new gene of the HNF4 family with distinct activation and expression profiles in oogenesis and embryogenesis of Xenopus laevisB Holewa
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Germany
Mol Cell Biol 17:687-94. 1997..All of these distinct characteristics of the newly identified HNF4 protein imply that the alpha and beta isoform have different functions in development and in adult tissues... - The nephrogenic potential of the transcription factors osr1, osr2, hnf1b, lhx1 and pax8 assessed in Xenopus animal capsChristiane Drews
Institut für Zellbiologie Tumorforschung Universitätsklinikum Essen, Universitat Duisburg Essen, Hufelandstrasse 55, 45122 Essen, Germany
BMC Dev Biol 11:5. 2011..Treatment of the undifferentiated animal pole explant (animal cap) of Xenopus with activin A and retinoic acid induces pronephros formation providing a powerful tool to analyze key molecular events in nephrogenesis... - Red fluorescent Xenopus laevis: a new tool for grafting analysisChristoph Waldner
Institut für Zellbiologie Tumorforschung, Universitat Duisburg Essen, D 45122 Essen, Germany
BMC Dev Biol 9:37. 2009..To track more than one event in the same animal, fluorescent markers that clearly differ in their emission spectrum are needed... - A distant upstream promoter of the HNF-4alpha gene connects the transcription factors involved in maturity-onset diabetes of the youngH Thomas
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Hufelandstrasse 55, D 45122 Essen, Germany
Hum Mol Genet 10:2089-97. 2001..25). These data suggest a regulatory network of the four MODY transcription factors interconnected at the distant upstream P2 promoter of the HNF-4alpha gene... - Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevisS Bartkowski
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Germany
Mol Cell Biol 13:421-31. 1993..The early appearance of XLFB1 expression during embryogenesis suggests that the tissue-specific transcription factor XLFB1 is involved in the determination and/or differentiation of specific cell types during organogenesis... - An alternative splice variant of the tissue specific transcription factor HNF4alpha predominates in undifferentiated murine cell typesH Nakhei
Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122 Essen, Germany
Nucleic Acids Res 26:497-504. 1998..All these features suggest that the presence of distinct splice variants of HNF4alpha modulates the activity of HNF4alphain a cell type specific way... - The bifunctional protein DCoH/PCD, a transcription factor with a cytoplasmic enzymatic activity, is a maternal factor in the rat egg and expressed tissue specifically during embryogenesisE P Strandmann
Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Germany
Int J Dev Biol 42:53-9. 1998.... - ERH (enhancer of rudimentary homologue), a conserved factor identical between frog and human, is a transcriptional repressorE Pogge von Strandmann
, , Germany
Biol Chem 382:1379-85. 2001..Furthermore, the DCoH/PCD coactivation of a HNF1 responsive reporter is inhibited by XERH. We propose that XERH is a cell type-specific transcriptional repressor, probably interfering with HNF1-dependent gene regulation via DCoH/PCD... - Ectopic pigmentation in Xenopus in response to DCoH/PCD, the cofactor of HNF1 transcription factor/pterin-4alpha-carbinolamine dehydrataseE Pogge v Strandmann
Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122, Essen, Germany
Mech Dev 91:53-60. 2000.... - Genomic structure of the Xenopus laevis liver transcription factor LFB1D Zapp
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Germany
Gene 134:251-6. 1993..This is in agreement with the structure found in the rat gene and supports the notion that the LFB1 homeobox evolved separately from the other genes encoding homeodomain proteins... - Misexpression of Xsiah-2 induces a small eye phenotype in XenopusS Bogdan
Institute of Cell Biology, University of Essen, Hufelandstrasse 55, D 45122 Essen, Germany
Mech Dev 103:61-9. 2001..As this phenotype has been also described for flies expressing sina ectopically, the data demonstrate the functional and structural conservation of Xsiah-2 and sina in metazoan eye development... - Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genesT Drewes
Institut für Zelbiologie, Universitatsklinikum Essen, Germany
Mol Cell Biol 16:925-31. 1996..Therefore, the differential expression of distinct HNF4 proteins may play a key role in the differential transcriptional regulation of HNF4-dependent genes... - Improved conditional expression systems resulting in physiological level of HNF4alpha expression confirm HNF4alpha induced apoptosis in the pancreatic beta-cell line INS-1Sabine Senkel
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat, Duisburg Essen, D 45122 Essen, Germany
BMC Res Notes 2:210. 2009..However, leakiness of basal transgene expression and artificially high expression level upon tetracycline addition may be disadvantageous... - Marking transgenic Xenopus froglets with passive micro transpondersChristoph Waldner
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Hufelandstrasse 55, Essen, Germany
Transgenic Res 16:539-40. 2007..This new technique makes the breeding of transgenic frogs most efficient, because the frogs do not have to be kept separately and they grow much faster when kept together in large containers... - Evidence for haploinsufficiency of the human HNF1alpha gene revealed by functional characterization of MODY3-associated mutationsHeike Thomas
Universitatsklinikum Essen, Institut für Zellbiologie Tumorforschung, Hufelandstrasse 55, D 45122 Essen, Germany
Biol Chem 383:1691-700. 2002..Our results suggest that haploinsufficiency of HNF1alpha is responsible for the pathogenesis of MODY3... - Aging of Xenopus tropicalis eggs leads to deadenylation of a specific set of maternal mRNAs and loss of developmental potentialAnna Kosubek
Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Universitat Duisburg Essen, Essen, Germany
PLoS ONE 5:e13532. 2010..Based on this hypothesis the developmental consequences of downregulation of specific transcripts can be analyzed in future... - Double conditional human embryonic kidney cell line based on FLP and ΦC31 mediated transgene integrationChristoph Waldner
Institut für Zellbiologie Tumorforschung, Universitat Duisburg Essen, Hufelandstr, 55, D 45122 Essen, Germany
BMC Res Notes 4:420. 2011..abstract:.. - FLP and Cre recombinase function in Xenopus embryosD Werdien
, , Hufelandstrasse 55, D-45122 Essen, Germany
Nucleic Acids Res 29:E53-3. 2001....