Research Topics
Genomes and Genes | Peter RehlingSummaryAffiliation: University of Freiburg Country: Germany Publications
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Publications
Insertion of hydrophobic membrane proteins into the inner mitochondrial membrane--a guided tourPeter Rehling
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Str 7, D 79104 Freiburg, Germany
J Mol Biol 326:639-57. 2003..The identification of components of these complexes and the analysis of the molecular mechanisms underlying their function are currently an exciting and fast developing field of molecular cell biology...- Mitochondrial import and the twin-pore translocasePeter Rehling
, , Hermann-Herder-Strasse 7, D-79104 Freiburg, Germany
Nat Rev Mol Cell Biol 5:519-30. 2004 
Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sortingAgnieszka Chacinska
Institut fur Biochemie und Molekularbiologie, ZBMZ, Stefan Meier Strasse 17, 79104 Freiburg, Germany
Mol Cell Biol 30:307-18. 2010..Depending on the signals of the preproteins, switches between the different forms of supercomplex and TIM23 are required for the completion of preprotein import...- Pam16 has an essential role in the mitochondrial protein import motorAnn E Frazier
Institut fur Biochemie und Molekularbiologie, und Fakultät für Biologie, Universitat Freiburg, D 79104 Freiburg, Germany
Nat Struct Mol Biol 11:226-33. 2004..Thus, Pam16 is a newly identified type of motor subunit and is required to promote a functional PAM reaction cycle, thereby driving preprotein import into the matrix... - Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17Agnieszka Chacinska
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
Cell 120:817-29. 2005..Thus, the presequence translocase is not a static complex but switches between TOM tethering and PAM binding in a reaction cycle involving Tim21 and Tim17... - Pam17 is required for architecture and translocation activity of the mitochondrial protein import motorMartin van der Laan
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Germany
Mol Cell Biol 25:7449-58. 2005..Our findings suggest that Pam17 is required for the correct organization of the Pam16-Pam18 complex and thus contributes to regulation of mtHsp70 activity at the inner membrane translocation site... - Coa3 and Cox14 are essential for negative feedback regulation of COX1 translation in mitochondriaDavid U Mick
Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, D 79104 Freiburg, Germany
J Cell Biol 191:141-54. 2010..Our data indicate that Coa1 binding to sequestered Mss51 in complex with Cox14, Coa3, and Cox1 is essential for full inactivation... - Mitochondrial protein import motor: differential role of Tim44 in the recruitment of Pam17 and J-complex to the presequence translocaseDana P Hutu
Institut fur Biochemie und Molekularbiologie, ZBMZ, Germany
Mol Biol Cell 19:2642-9. 2008..We suggest that Tim44 is not simply a scaffold for binding of motor subunits but plays a differential role in the recruitment of PAM modules to the inner membrane translocase... - A role for Tim21 in membrane-potential-dependent preprotein sorting in mitochondriaMartin van der Laan
Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
Curr Biol 16:2271-6. 2006..These findings suggest a new coupling mechanism in an energy-transducing membrane... - Shy1 couples Cox1 translational regulation to cytochrome c oxidase assemblyDavid U Mick
Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, Freiburg, Germany
EMBO J 26:4347-58. 2007..We suggest that Shy1 links Cox1 translational regulation to complex IV assembly and supercomplex formation... - The assembly pathway of the mitochondrial carrier translocase involves four preprotein translocasesKarina Wagner
Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, D 79104 Freiburg, Germany
Mol Cell Biol 28:4251-60. 2008.... - Assembly of the three small Tim proteins precedes docking to the mitochondrial carrier translocaseNatalia Gebert
Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Freiburg, Germany
EMBO Rep 9:548-54. 2008.... - Sorting switch of mitochondrial presequence translocase involves coupling of motor module to respiratory chainNils Wiedemann
Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Freiburg, Germany
J Cell Biol 179:1115-22. 2007..The motor does not assemble en bloc with the translocase but apparently in a step-wise manner with the Pam16/18 module before the Hsp70 core... - Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplexAgnieszka Chacinska
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
EMBO J 22:5370-81. 2003..Thus, Tim50 functions as a dynamic factor and the IMS domain of Tom22 represents a stabilizing element in formation of a productive translocation contact site supercomplex... - A J-protein is an essential subunit of the presequence translocase-associated protein import motor of mitochondriaKaye N Truscott
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
J Cell Biol 163:707-13. 2003..We conclude that the reaction cycle of the PAM of mitochondria involves an essential J-protein... - Motor-free mitochondrial presequence translocase drives membrane integration of preproteinsMartin van der Laan
Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
Nat Cell Biol 9:1152-9. 2007..Thus, the minimal system for preprotein integration into the mitochondrial inner membrane is the presequence translocase, a cardiolipin-rich membrane and a membrane potential... 
Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membraneNatalia Gebert
Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
Mol Cell 44:811-8. 2011..We conclude that the assembly of Sdh3 with different partner proteins, Sdh4 and Tim18, recruits it to two different mitochondrial membrane complexes with functions in bioenergetics and protein biogenesis, respectively...- Mitochondria use different mechanisms for transport of multispanning membrane proteins through the intermembrane spaceAnn E Frazier
, , D-79104 Freiburg, Germany
Mol Cell Biol 23:7818-28. 2003..We conclude that mitochondria employ different mechanisms for translocation of multispanning proteins across the aqueous intermembrane space... - Protein insertion into the mitochondrial inner membrane by a twin-pore translocasePeter Rehling
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
Science 299:1747-51. 2003..Thus, protein insertion was driven by the coordinated action of a twin-pore complex in two voltage-dependent steps... - Mdm38 interacts with ribosomes and is a component of the mitochondrial protein export machineryAnn E Frazier
, , D-79104 Freiburg, Germany
J Cell Biol 172:553-64. 2006.... - The carboxyl-terminal third of the dicarboxylate carrier is crucial for productive association with the inner membrane twin-pore translocaseKatrin Brandner
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
J Biol Chem 280:6215-21. 2005..We concluded that, in this case, a single structural repeat can drive inner membrane insertion, whereas all three related units contribute targeting information for outer membrane translocation... - The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channelAndreas Geissler
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, D 79104, Freiburg, Germany
Cell 111:507-18. 2002..Thus, Tim50 is crucial for guiding the precursors of matrix proteins to their insertion site in the inner membrane... - Dual role of mitofilin in mitochondrial membrane organization and protein biogenesisKarina von der Malsburg
Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
Dev Cell 21:694-707. 2011..Our findings indicate that mitofilin is a central component of MINOS and functions as a multifunctional regulator of mitochondrial architecture and protein biogenesis... - On the mechanism of preprotein import by the mitochondrial presequence translocaseMartin van der Laan
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
Biochim Biophys Acta 1803:732-9. 2010..In this review, we discuss recent advances in our understanding of the mechanisms of matrix translocation and membrane insertion by the TIM23 machinery... - Mitochondrial preprotein translocases as dynamic molecular machinesMartin van der Laan
, , Freiburg, Germany
FEMS Yeast Res 6:849-61. 2006..These two molecular machineries mediate the multistep import of preproteins with cleavable N-terminal signal sequences into the matrix or inner membrane of mitochondria... - The translocator maintenance protein Tam41 is required for mitochondrial cardiolipin biosynthesisStephan Kutik
Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, 79104 Freiburg, Germany
J Cell Biol 183:1213-21. 2008..The pleiotropic effects of the translocator maintenance protein on preprotein import and respiratory chain can be attributed to its role in biosynthesis of mitochondrial cardiolipin... - Mitochondrial F1Fo-ATP synthase: the small subunits e and g associate with monomeric complexes to trigger dimerizationKarina Wagner
Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
J Mol Biol 392:855-61. 2009..We conclude that association of Su e and Su g with monomeric F(1)F(o)-ATP synthase represents an initial step of oligomer formation... - Mitochondrial cardiolipin involved in outer-membrane protein biogenesis: implications for Barth syndromeNatalia Gebert
Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, 79104 Freiburg, Germany
Curr Biol 19:2133-9. 2009..Our findings reveal a new role for cardiolipin in protein sorting at the mitochondrial outer membrane and bear implications for the pathogenesis of Barth syndrome... - Protein transport machineries for precursor translocation across the inner mitochondrial membraneKarina Wagner
Institut fur Biochemie und Molekularbiologie, ZBMZ, Universitat Freiburg, D 79104 Freiburg, Germany
Biochim Biophys Acta 1793:52-9. 2009..Here we describe the organization of the TIM complexes and discuss the current models as to how they mediate the posttranslational import of proteins across and into the inner mitochondrial membrane... - Taz1, an outer mitochondrial membrane protein, affects stability and assembly of inner membrane protein complexes: implications for Barth SyndromeKatrin Brandner
Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
Mol Biol Cell 16:5202-14. 2005..We conclude that inactivation of Taz1 affects both assembly and stability of respiratory chain complexes in the inner membrane of mitochondria... - Import of precursor proteins into isolated yeast mitochondriaNils Wiedemann
, , Germany
Methods Mol Biol 313:373-83. 2006..The process of mitochondrial protein import can be reconstituted in vitro using isolated mitochondria and in vitro synthesized precursor proteins... - The presequence translocase-associated protein import motor of mitochondria. Pam16 functions in an antagonistic manner to Pam18Yanfeng Li
, , D-79104 Freiburg, Germany
J Biol Chem 279:38047-54. 2004..Pam16 represents a new type of cochaperone that controls the stimulatory effect of the J-protein Pam18 and regulates the interaction of mtHsp70 with precursor proteins during import into mitochondria... - Cooperation of stop-transfer and conservative sorting mechanisms in mitochondrial protein transportMaria Bohnert
Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung ZBMZ Fakultät für Biologie, Universitat Freiburg, 79104 Freiburg, Germany
Curr Biol 20:1227-32. 2010..We conclude that the multispanning protein is inserted in a modular manner by the coordinated action of two inner-membrane preprotein translocases... - Mitochondrial import of the ADP/ATP carrier: the essential TIM complex of the intermembrane space is required for precursor release from the TOM complexKaye N Truscott
, , D-79104 Freiburg, Germany
Mol Cell Biol 22:7780-9. 2002.... - Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complexMilena Vukotic
Department of Biochemistry II, University of Gottingen, D 37073 Gottingen, Germany
Cell Metab 15:336-47. 2012..Our analyses reveal that the cytochrome oxidase of mitochondria displays intrinsic heterogeneity with regard to its subunit composition and that distinct forms of respirasomes can be formed by complex variants... - Pex8p: an intraperoxisomal organizer of the peroxisomal import machineryBirgit Agne
Abteilung für Zellbiochemie, Ruhr Universitat Bochum, D 44780, Bochum, Germany
Mol Cell 11:635-46. 2003..We conclude that Pex8p organizes the formation of the larger import complex from the trans side of the peroxisomal membrane and thus might enable functional communication between both sides of the membrane... - Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channelPeter Kovermann
Biophysik, Universitat Osnabruck, FB Biologie Chemie, D 49034 Osnabrück, Germany
Mol Cell 9:363-73. 2002..Thus, a protein insertion complex can combine three essential functions, signal recognition, channel formation, and energy transduction, in one central component... - Mitochondrial biogenesis: is an old dog still teaching us new tricks? Meeting on the Assembly of the Mitochondrial Respiratory ChainRosemary A Stuart
Department of Biological Sciences, Marquette University, 530 N 15th Street, Milwaukee, WI 53233, USA
EMBO Rep 9:33-8. 2008 - The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranesReinhard Albrecht
Max Planck Institut fur Biochemie, Abteilung Membranbiochemie, Am Klopferspitz 18, D 82512 Martinsried, Germany
EMBO Rep 7:1233-8. 2006..We also identified a core sequence of Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid residues of Tom22 are important for binding to Tim21. Here we suggest a mechanism for the TOM-TIM interaction... - Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthaseJörg Reinders
Rudolf Virchow Center Deutsche Forschungsgemeinschaft Research Center for Experimental Biomedicine, Universitat Wurzburg, D 97078 Wurzburg, Germany
Mol Cell Proteomics 6:1896-906. 2007..The authentic phosphoproteome of yeast mitochondria will represent a rich source to uncover novel roles of reversible protein phosphorylation... - Tim50 maintains the permeability barrier of the mitochondrial inner membraneMichael Meinecke
Biophysik, Universitat Osnabruck, FB Biologie Chemie, D 49034 Osnabrück, Germany
Science 312:1523-6. 2006..Thus, the hydrophilic cis domain of Tim50 maintains the permeability barrier of mitochondria by closing the translocation pore in a presequence-regulated manner... - The proteome of Saccharomyces cerevisiae mitochondriaAlbert Sickmann
Rudolf-Virchow-Center for Experimental Biomedicine, , Versbacher Strasse 9, , Germany
Proc Natl Acad Sci U S A 100:13207-12. 2003..The mitochondrial proteome will provide an important database for the analysis of new mitochondrial and mitochondria-associated functions and the characterization of mitochondrial diseases...