Research Topics
| Sven PoppertSummaryAffiliation: University of Ulm Country: Germany Publications
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Detail Information
Publications
Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridizationSven Poppert
Department of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany
J Clin Microbiol 43:3390-7. 2005..FISH is less sensitive but is useful for the identification of CSF samples showing bacteria in the Gram stain. Based on our results, an approach for laboratory diagnosis of meningitis including PCR and FISH is discussed...
Evaluation of the Merlin MICRONAUT system for rapid direct susceptibility testing of gram-positive cocci and gram-negative bacilli from positive blood culturesNele Wellinghausen
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany
J Clin Microbiol 45:789-95. 2007..Thereby, it reduces the time to results of blood culture testing and may have a positive impact on patient care...
Identification of thermotolerant campylobacter species by fluorescence in situ hybridizationSven Poppert
Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany
J Clin Microbiol 46:2133-6. 2008..The assay showed high degrees of sensitivity for the identification of C. jejuni (90%), C. coli (97%), C. lari (81%), and C. upsaliensis (100%) to the species level...
Rapid detection of Brucella spp. in blood cultures by fluorescence in situ hybridizationNele Wellinghausen
Department of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Koch-Str. 8, 89081 Ulm, Germany
J Clin Microbiol 44:1828-30. 2006..We describe a new 16S rRNA-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of Brucella and that can be applied directly to positive blood cultures...
Superiority of molecular techniques for identification of gram-negative, oxidase-positive rods, including morphologically nontypical Pseudomonas aeruginosa, from patients with cystic fibrosisNele Wellinghausen
Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Str 8, D 89081 Ulm, Germany
J Clin Microbiol 43:4070-5. 2005..In addition, real-time PCR and FISH allowed identification of morphologically nontypical isolates of P. aeruginosa within a few hours...
Rapid identification of Fusobacterium nucleatum and Fusobacterium necrophorum by fluorescence in situ hybridizationAnja Sigge
Department of Medical Microbiology and Hygiene, University Hospital of Ulm, D 89081 Ulm, Germany
Diagn Microbiol Infect Dis 58:255-9. 2007..Preliminary results show that the method offers the perspective for direct detection of these pathogens in blood cultures and abscess aspirates...
Chlamydia pneumoniae in an ex vivo human artery culture modelSven Poppert
Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Str 8, 89081 Ulm, Germany
Atherosclerosis 187:50-6. 2006..The presence of chlamydial LPS in the absence of viable organisms within the artery vessel wall may explain the failure of antibiotic treatment strategies for atherosclerosis...
Rapid identification of yeast by fluorescence in situ hybridisation from broth and blood culturesHagen Frickmann
Department of Tropical Medicine, Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Hamburg, Germany
Mycoses 55:521-31. 2012..Given this good sensitivity and specificity, FISH may be applied for rapid identification of yeast in screening analyses, thus giving the opportunity for more precise targeting of antimycotic therapy...
Evaluation of new selective culture media and a rapid fluorescence in situ hybridization assay for identification of Clostridium difficile from stool samplesKathrin Bloedt
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany
J Med Microbiol 58:874-7. 2009..0, 42.6 and 90.4 %, respectively. In addition, a C. difficile-specific fluorescence in situ hybridization assay was developed, facilitating rapid and reliable identification of cultured isolates...
Evaluation of fluorescence in situ hybridisation (FISH) for the identification of Candida albicans in comparison with three phenotypic methodsAnna Lakner
Institute of Medical Microbiology and Hygiene, University of Ulm, Germany
Mycoses 55:e114-23. 2012..albicans isolates within 3 h with a sensitivity and specificity of 100%. FISH was additionally applied to 48 blood cultures showing yeasts in the Gram stain and correctly identified all 33 cases of C. albicans...
Detection and differentiation of chlamydiae by fluorescence in situ hybridizationSven Poppert
, , Ulm, Germany
Appl Environ Microbiol 68:4081-9. 2002..In contrast to previously established detection methods for chlamydiae, FISH was not susceptible to false-positive results and allows the detection of all recognized chlamydiae in one single step...
Fluorescence in situ hybridization for rapid identification of Achromobacter xylosoxidans and Alcaligenes faecalis recovered from cystic fibrosis patientsNele Wellinghausen
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert Koch Str 8, D 89081 Ulm, Germany
J Clin Microbiol 44:3415-7. 2006..Both assays showed high sensitivities and high specificities with a collection of 155 nonfermenters from CF patients...
Detection of mutations conferring resistance to linezolid in Enterococcus spp. by fluorescence in situ hybridizationGuido Werner
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert Kochstr 8, D 89081 Ulm, Germany
J Clin Microbiol 45:3421-3. 2007..The assay was evaluated with 106 Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify a single mutated allele in phenotypically linezolid-susceptible isolates...
Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridizationNele Wellinghausen
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert Koch Str 8, D 89081 Ulm, Germany
J Clin Microbiol 45:3424-6. 2007..faecium, E. gallinarum, the VanC-type resistance group) was developed and evaluated with 33 reference strains, 68 clinical isolates, and 58 positive blood cultures. All probes showed excellent sensitivities and specificities...
Recovery of an environmental Chlamydia strain from activated sludge by co-cultivation with Acanthamoeba spAstrid Collingro
Division of Microbial Ecology, Institute for Ecology and Conservation Biology, University of Vienna, Althanstr. 14, 1090 Vienna, Austria
Microbiology 151:301-9. 2005....
