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Genomes and Genes | Olaf PongsSummaryAffiliation: University of Hamburg Country: Germany Publications
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Regulation of excitability by potassium channelsO Pongs
Institute for Neural Signal Conduction, Center for Molecular Neurobiology, University Hospital Hamburg Eppendorf, Martinistr 52, 20246 Hamburg, Germany
Results Probl Cell Differ 44:145-61. 2008..It is likely that these Kv channels play a prominent role in both propagating and integrating dendritic signaling, as well as axonal action-potential firing and propagation...
Ins and outs of cardiac voltage-gated potassium channelsOlaf Pongs
Institut für Neurale Signalverarbeitung, Centre for Molecular Neurobiology, University Hospital Eppendorf, Martinistr 52, 20246 Hamburg, Germany
Curr Opin Pharmacol 9:311-5. 2009....- Ancillary subunits associated with voltage-dependent K+ channelsOlaf Pongs
Institut für Neurale Signalverarbeitung, Zentrum fur Molekulare Neurobiologie Hamburg, Universitatsklinikum Hamburg Eppendorf, Universitat Hamburg, Hamburg, Germany
Physiol Rev 90:755-96. 2010..We critically review present knowledge on the physiological role of ancillary Kv channel subunits and their effects on Kv channel properties... - Ion Channel Target--Informa conferenceOlaf Pongs
University Medical Center, Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany
IDrugs 11:318-21. 2008 - Coexpression of the KCNA3B gene product with Kv1.5 leads to a novel A-type potassium channelT Leicher
Institut für Neurale Signalverarbeitung, Zentrum fur Molekulare Neurobiologie Hamburg, Martinistrasse 52, 20246 Hamburg, Germany
J Biol Chem 273:35095-101. 1998..Thus, the expression of Kvbeta3.1 subunits potentially extends the possibilities to express diverse A-type Kv channels in the human brain... - Characterization of human Kv4.2 mediating a rapidly-inactivating transient voltage-sensitive K+ currentX R Zhu
Institut für Neurale Signalverarbeitung, Zentrum fur Molekulare Neurobiologie Hamburg, Germany
Receptors Channels 6:387-400. 1999..In contrast we did not detect Kv4.2. but solely Kv4.3 mRNA in human heart RNA preparations. This may suggest that Kv4.2 subunits do not contribute to the rapid transient outward K+ current of atrial and ventricular myocytes in humans... - Modulation of Kv4.2 channels by a peptide isolated from the venom of the giant bird-eating tarantula Theraphosa leblondiJan Ebbinghaus
, , Martinistrasse 52, Hamburg 20246, Germany
Toxicon 43:923-32. 2004..1 channel was replaced by the corresponding Kv4.2 domain, were sensitive to TLTx1. Apparently, TLTx1 can act as a gating modifier of Kv4.2 channels... - Structural and functional characterization of Kv6.2 a new gamma-subunit of voltage-gated potassium channelX R Zhu
Institut für Neurale Signalverarbeitung, Zentrum fur Molekulare Neurobiologie Hamburg, Germany
Receptors Channels 6:337-50. 1999..1/Kv6.2 channels was their submicromolar sensitivity to the antiarrhythmic drug propafenone. The data suggest that delayed-rectifier type channels containing Kv6.2 subunits may contribute to cardiac action potential repolarization... 
Functional expression of a rat homologue of the voltage gated either á go-go potassium channel reveals differences in selectivity and activation kinetics between the Drosophila channel and its mammalian counterpartJ Ludwig
ZMNH, Institut für Neurale Signalverarbeitung, Hamburg, Germany
EMBO J 13:4451-8. 1994..This novel K channel property may have important implications in neural signal transduction allowing neurons to tune their repolarizing properties in response to membrane hyperpolarization...- Conserved Kv4 N-terminal domain critical for effects of Kv channel-interacting protein 2.2 on channel expression and gatingR Bähring
Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Universitat Hamburg, Martinistrasse 52, 20246 Hamburg, Germany
J Biol Chem 276:23888-94. 2001..We propose that an efficient transport of Kv4 channels to the cell surface depends on KChIP binding to the Kv4 N-terminal domain. Our data suggest that the binding is necessary, but not sufficient, for the functional activity of KChIPs... - C-terminal HERG (LQT2) mutations disrupt IKr channel regulation through 14-3-3epsilonChi Un Choe
Institute for Neural Signal Transduction, ZMNH, Department of Pediatrics, University Hospital Hamburg Eppendorf, Hamburg, Germany
Hum Mol Genet 15:2888-902. 2006..In summary, the attenuated functional effects of 14-3-3epsilon on C-terminally truncated HERG channels demonstrate the physiological importance of coupling beta-adrenergic stimulation and HERG channel activity... - RERG is a molecular correlate of the inward-rectifying K current in clonal rat pituitary cellsC K Bauer
Physiologisches Instiut, Universitats Krankenhaus Eppendorf, Hamburg, Germany
Receptors Channels 6:19-29. 1998..The voltage- and time-dependent properties of both currents were found to be almost identical. These results strongly suggest that RERG channels mediate IK, IR in GH3/B6 cells... - Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunitJ Rettig
Zentrum fur Molekulare Neurobiologie Hamburg, Institut für Neurale Signalverarbeitung, Germany
Nature 369:289-94. 1994..This effect is mediated by an inactivating ball domain in the Kv beta 1 amino terminus... - Cloning and functional expression of rat eag2, a new member of the ether-à-go-go family of potassium channels and comparison of its distribution with that of eag1J Ludwig
Institut für Neurale Signalverarbeitung, Zentrum fur Molekulare Neurobiologie Hamburg, Martinistrasse 52, Hamburg, D 20246, Germany
Mol Cell Neurosci 16:59-70. 2000..Thus, in neurones, this current is likely to impart a modulation in membrane conductance, which is sensitively responsive to resting internal calcium, and levels of electrical activity... - Impaired endocytosis of the ion channel TRPM4 is associated with human progressive familial heart block type IMartin Kruse
Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Universitat Hamburg, Hamburg, Germany
J Clin Invest 119:2737-44. 2009..Our data therefore revealed a gain-of-function mechanism underlying this type of familial heart block... - N-type inactivation features of Kv4.2 channel gatingManuel Gebauer
, , 20246 Hamburg, Germany
Biophys J 86:210-23. 2004..2/KChIP2.1 patch currents. However, double mutant cycle analysis of N-terminal inactivating and pore domains indicated differences in the energetics and structural determinants between Kv4.2 and Shaker N-type inactivation... - Conditional transgenic suppression of M channels in mouse brain reveals functions in neuronal excitability, resonance and behaviorH Christian Peters
Institut für Neurale Signalverarbeitung, Zentrum fur Molekulare Neurobiologie Hamburg, Martinistrasse 52, 20246 Hamburg, Germany
Nat Neurosci 8:51-60. 2005..M channels are thus critical determinants of cellular and neuronal network excitability, postnatal brain development and cognitive performance... - hKCNMB3 and hKCNMB4, cloning and characterization of two members of the large-conductance calcium-activated potassium channel beta subunit familyR Behrens
Institut für Neurale Signalverarbeitung, ZMNH, Universitat Hamburg, Martinistr 52, D 20246, Hamburg, Germany
FEBS Lett 474:99-106. 2000..BKalpha/beta4 channels were not blocked by 100 nM charybdotoxin or iberiotoxin, and were activated by 17beta-estradiol... - Cloning and characterization of a human delayed rectifier potassium channel geneB Albrecht
Zentrum für Molekulare Neurobiologie ZMNH, Institut für Neurale Signalverarbeitung, UKE Haus 42, Hamburg, Germany
Receptors Channels 1:99-110. 1993..Obviously, these domains of Kv 2.1 channels influence biophysical K+ channel properties, which are thought to be determined solely by the membrane spanning core domain of potassium channels... - A recessive C-terminal Jervell and Lange-Nielsen mutation of the KCNQ1 channel impairs subunit assemblyN Schmitt
Institut fuer Neurale Signalverarbeitung, ZMNH, Martinistrasse 52, 20246 Hamburg, Germany
EMBO J 19:332-40. 2000..The results provide a molecular basis for the clinical observation that heterozygous JLN carriers show slight cardiac dysfunctions and that the severe JLNS phenotype is characterized by the absence of KvLQT1 channel... - Characterization of a voltage-activated K-channel gene cluster on human chromosome 12p13B Albrecht
Instut für neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Hamburg, Germany
Receptors Channels 3:213-20. 1995..Northern blot analyses of the KCNA1, KCNA5 and KCNA6 mRNAs in different human brain areas show that the genes are distinctly expressed. Therefore, they may be transcribed independently of each other... - Identification and functional characterization of a novel KCNE2 (MiRP1) mutation that alters HERG channel kineticsDirk Isbrandt
Institute for Neural Signal Transduction, Centre for Molecular Neurobiology Hamburg, University of Hamburg, Martinistrasse 52, 20246 Hamburg, Germany
J Mol Med (Berl) 80:524-32. 2002.... - Voltage-gated potassium channels: from hyperexcitability to excitementO Pongs
ZMNH, Institut für Neurale Signalverarbeitung, Hamburg, Germany
FEBS Lett 452:31-5. 1999..Most likely, more voltage-activated potassium channel genes will be linked to related disorders in the near future... - Functional and molecular aspects of voltage-gated K+ channel beta subunitsO Pongs
Institut für Neurale Signalverarbeitung Center for Molecular Neurobiology, Hamburg, Germany
Ann N Y Acad Sci 868:344-55. 1999..1 subunits leads to a reduction of A-type Kv channel activity in hippocampal and striatal neurons of knock-out mice. This reduction may be correlated with altered cognition and motor control in the knock-out mice... - Kinetic analysis of open- and closed-state inactivation transitions in human Kv4.2 A-type potassium channelsR Bähring
Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie der Universität Hamburg, 20246 Hamburg, Germany
J Physiol 535:65-81. 2001..2 channels accumulate in the closed-inactivated state(s), from which they directly recover, bypassing the open state... - Coupling of voltage-dependent potassium channel inactivation and oxidoreductase active site of Kvbeta subunitsR Bähring
Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie der Universität Hamburg, Hamburg, Germany
J Biol Chem 276:22923-9. 2001..1 to confer rapid inactivation to Kv1.5 channels in Xenopus oocytes. We propose that Kvbeta oxidoreductase activity couples Kv channel inactivation to cellular redox regulation... - Gene structures and expression profiles of three human KCND (Kv4) potassium channels mediating A-type currents I(TO) and I(SA)D Isbrandt
Institute of Neural Signal Transduction, Centre for Molecular Neurobiology, Martinistrasse 52, Hamburg, 20246, Germany
Genomics 64:144-54. 2000..The KCND3 gene contains an additional exon of 57 bp, which is not present in the other two KCND genes and gives rise to the C-terminal splice KCND3L variant with an insertion of 19 amino acids... - Ultrastructural localization of Shaker-related potassium channel subunits and synapse-associated protein 90 to septate-like junctions in rat cerebellar PinceauxG Laube
Zentrum für Molekulare Neurobiologie, Universitat Hamburg, Germany
Brain Res Mol Brain Res 42:51-61. 1996..The results suggest that Kv1.1 and Kv1.2 form heterooligomeric delayed rectifier type Kv channels, being colocalized to septate-like junctions by interaction with SAP90... - Toxin binding to chimeric K+ channels immobilised on a solid nitrocellulose supportChristian Legros
ZMNH, Universitat Hamburg, Zentrum für Molekulare Neurobiologie, Institut für Neurale Signalverarbeitung, Falkenried 94, 20251 Hamburg, Germany
Biochem Biophys Res Commun 353:1086-90. 2007..Their behaviour in solution was compared with that when spotted on a nitrocellulose-supported film and their responses to selective high affinity ligands, including polypeptide toxins and TEA, were studied... - Cloning and functional expression of rat ether-à-go-go-like K+ channel genesB Engeland
Zentrum für Molekulare Neurobiologie, Institut für Neurale Signalverarbeitung, Martinistrasse 52, D 20246 Hamburg, Germany
J Physiol 513:647-54. 1998..5. RELK2 channels produced currents with a fast inactivation component and HERG-like tail currents. RELK2 currents were not sensitive to the HERG channel blocker E4031... - TRPM4 cation channel mediates axonal and neuronal degeneration in experimental autoimmune encephalomyelitis and multiple sclerosisBenjamin Schattling
Forschergruppe Neuroimmunologie, Zentrum für Molekulare Neurobiologie, Universitatsklinikum Hamburg Eppendorf, Hamburg, Germany
Nat Med 18:1805-11. 2012..Electrophysiological recordings revealed TRPM4-dependent neuronal ion influx and oncotic cell swelling upon excitotoxic stimulation. Therefore, interference with TRPM4 could translate into a new neuroprotective treatment strategy... - Structural and functional characterization of human potassium channel subunit beta 1 (KCNA1B)T Leicher
Zentrum für Molekulare Neurobiologie, Institut für Neurale Signalverarbeitung, Hamburg, Germany
Neuropharmacology 35:787-95. 1996..It is also shown that the amino terminal Kv beta 1.1 and Kv 1.4 alpha inactivating domains compete with each other, probably for the binding to the same receptor site(s) on Kv 1 alpha-subunits... - Effects of fluoroquinolones on HERG currentsU Bischoff
GENION Forschungsgesellschaft, Abteistrasse 57, 20149, Hamburg, Germany
Eur J Pharmacol 406:341-3. 2000..5+/-0.8, 41. 2+/-2.0 and 37.5+/-3.3 microg/ml, respectively. Current inhibitions were reversible after washout of the compounds. By contrast, ciprofloxacin at concentrations of up to 100 microg/ml did not effect HERG outward currents... - Presynaptic potassium channelsJ Roeper
Zentrum für Molekulare Neurobiologie, Institut für Neurale Signalverarbeitung, Martinistrasse 52, Haus 42, D 20246 Hamburg, Germany
Curr Opin Neurobiol 6:338-41. 1996.... - Mice with disrupted BK channel beta1 subunit gene feature abnormal Ca(2+) spark/STOC coupling and elevated blood pressureS Plüger
Institut für Neurale Signalverarbeitung, ZMNH, Universitat Hamburg, Hamburg, Germany
Circ Res 87:E53-60. 2000..We propose that the elevated blood pressure in BKbeta1 -/- mice serves to normalize Ca(2+) spark/STOC coupling for regulating myogenic tone. The full text of this article is available at http://www.circresaha.org... - Differential modulation of Kv1 channel-mediated currents by co-expression of Kvbeta3 subunit in a mammalian cell-lineRobert Bähring
Institut für Neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie der Universität Hamburg, Germany
Mol Membr Biol 21:19-25. 2004..This suggests that the presence of an inactivating domain and a receptor in a channel pore, although necessary, may not be sufficient for an effective rapid N-type inactivation of Kv1 channels in heterologous expression systems... - Engineering-specific pharmacological binding sites for peptidyl inhibitors of potassium channels into KcsAChristian Legros
Institut für Neurale Signalverarbeitung, ZMNH, Universitat Hamburg, Martinistrasse 52, D 20246 Hamburg, Germany
Biochemistry 41:15369-75. 2002..X chimeras containing the subregion I of the corresponding mammalian Kv1.X channels. This innovative approach may facilitate the high-throughput screening of ligand libraries aimed at the discovery of novel potassium channel modulators... - Carboxy-terminal domain mediates assembly of the voltage-gated rat ether-à-go-go potassium channelJ Ludwig
Zentrum für Molekulare Neurobiologie, Universitat Hamburg, Germany
EMBO J 16:6337-45. 1997.... - Calmodulin is essential for cardiac IKS channel gating and assembly: impaired function in long-QT mutationsLiora Shamgar
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
Circ Res 98:1055-63. 2006.... - Contribution of N- and C-terminal Kv4.2 channel domains to KChIP interaction [corrected]Britta Callsen
, , Germany
J Physiol 568:397-412. 2005..Less coincidence of binding and functional modulation indicates a more loose 'anchoring' at T1- and C-terminal interaction sites. Our results refine and extend previously proposed structural models for Kv4.2/KChIP complex formation... - Effects of Kv1.2 intracellular regions on activation of Kv2.1 channelsAnnette Scholle
, , 07740 Jena, Germany
Biophys J 87:873-82. 2004..It is concluded that interactions between N-terminus, S4-S5 linker, and/or C-terminus modulate the activation time course of Kv2.1 channels and that part of these interactions is voltage dependent... - Novel gene hKCNE4 slows the activation of the KCNQ1 channelSiyong Teng
Sino German Laboratory for Molecular Medicine and Center for Molecular Cardiology, Fuwai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 167 Beilishilu, Beijing 100037, China
Biochem Biophys Res Commun 303:808-13. 2003..Electrophysiological study showed that hKCNE4 modulates the activation of the KCNQ1 channel... - Pacemaker channel dysfunction in a patient with sinus node diseaseEric Schulze-Bahr
Genetics of Arrhythmias, Molecular Cardiology Section, Institute for Arteriosclerosis Research, University of Munster, Munster, Germany
J Clin Invest 111:1537-45. 2003..Taken together, the clinical, genetic, and in vitro data provide a likely explanation for the patient's sinus bradycardia and the chronotropic incompetence... - Inhibition of human TREK-1 channels by bupivacaineMark A Punke
Department of Anesthesiology, University Hospital Hamburg-Eppendorf, Germany
Anesth Analg 96:1665-73, table of contents. 2003..Bupivacaine inhibited TREK-1 channels and depolarized the membrane potential of cells expressing TREK-1 channels in a concentration-dependent and reversible manner. Both effects may contribute to conductance block caused by bupivacaine... - The pore region of the Kv1.2alpha subunit is an important component of recombinant Kv1.2 channel oxygen sensitivityLaura Conforti
Division of Nephrology and Hypertension, Department of Internal Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267 0585, USA
Biochem Biophys Res Commun 306:450-6. 2003..Thus, the pore and its surrounding regions of Kv1.2 polypeptide confer its hypoxic inhibition. This response is independent on the redox modulation of methionine residues in this protein segment... - Characterization of a novel Long QT syndrome mutation G52R-KCNE1 in a Chinese familyLijuan Ma
Sino-German Laboratory for Molecular Medicine, Fuwai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 167 Beilishilu, Beijing 100037, China
Cardiovasc Res 59:612-9. 2003..This could underlie the molecular mechanism of ventricular arrhythmias and sudden death in those patients... - Clinical and electrophysiological characterization of a novel mutation R863X in HERG C-terminus associated with long QT syndromeSiyong Teng
Sino German Laboratory for Molecular Medicine and Center for Molecular Cardiology, Fuwai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 167 Beilishilu, 100037 Beijing, China
J Mol Med (Berl) 82:189-96. 2004..These findings provide new insights into the structure-function relationships of the HERG C-terminus... - Conservation of regulatory function in calcium-binding proteins: human frequenin (neuronal calcium sensor-1) associates productively with yeast phosphatidylinositol 4-kinase isoform, Pik1Thomas Strahl
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
J Biol Chem 278:49589-99. 2003..We propose, therefore, that the function of NCS-1 in mammals may closely resemble that of Frq1 in S. cerevisiae and, hence, that frequenins in general may serve as regulators of certain isoforms of phosphatidylinositol 4-kinase... - Isoform-specific regulation of mood behavior and pancreatic beta cell and cardiovascular function by L-type Ca 2+ channelsMartina J Sinnegger-Brauns
Abteilung Pharmakologie und Toxikologie, Institut fur Pharmazie, Universitat Innsbruck, Austria
J Clin Invest 113:1430-9. 2004..This animal model provides a useful tool to elucidate whether Ca(v)1.3-selective channel modulation represents a novel pharmacological approach to modify CNS function without major peripheral effects... - KCNQ1 channels sense small changes in cell volumeMorten Grunnet
Department of Medical Physiology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark
J Physiol 549:419-27. 2003..From our results we propose that KCNQ1 and KCNQ4 channels play an important role in cell volume control, e.g. during transepithelial transport of salt and water... - Toxin-induced conformational changes in a potassium channel revealed by solid-state NMRAdam Lange
Max Planck Institute for Biophysical Chemistry, Department of NMR-based Structural Biology, , Germany
Nature 440:959-62. 2006..We propose that structural flexibility of the K+ channel and the toxin represents an important determinant for the high specificity of toxin-K+ channel interactions... - A structural link between inactivation and block of a K+ channelChristian Ader
Max Planck Institute for Biophysical Chemistry, Department of NMR Based Structural Biology, Am Fassberg 11, 37077 Gottingen, Germany
Nat Struct Mol Biol 15:605-12. 2008..The results establish a structural link between inactivation and block of a K(+) channel in a membrane setting... - Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayersRobert Schneider
Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
J Am Chem Soc 130:7427-35. 2008.... - NADPH oxidase accounts for enhanced superoxide production and impaired endothelium-dependent smooth muscle relaxation in BKbeta1-/- miceMatthias Oelze
II.Medizinische Klinik, , Langenbeckstrasse 1, D-55131 Mainz, Germany
Arterioscler Thromb Vasc Biol 26:1753-9. 2006..CONCLUSIONS: The deletion of BKbeta1 causes endothelial dysfunction by increasing O2- formation via increasing activity and expression of the vascular NADPH oxidase... - Two-dimensional solid-state NMR applied to a chimeric potassium channelAdam Lange
Department for NMR-based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, , Germany
J Recept Signal Transduct Res 26:379-93. 2006..SsNMR experiments conducted at two different temperatures point to differential molecular dynamics of the channel... - The molecular mechanism of toxin-induced conformational changes in a potassium channel: relation to C-type inactivationUlrich Zachariae
Computational Biomolecular Dynamics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
Structure 16:747-54. 2008..Furthermore, our simulations indicate heterogeneity in the binding modes of Kaliotoxin, which might serve to enhance its affinity for KcsA-Kv1.3 further by entropic stabilization...