Kurt Pfannkuche

Summary

Affiliation: University of Cologne
Country: Germany

Publications

  1. doi request reprint Induced pluripotent stem cells: a new approach for physiological research
    Kurt Pfannkuche
    Institute for Neurophysiology, University of Cologne, Germany
    Cell Physiol Biochem 26:105-24. 2010
  2. pmc Human pluripotent stem cell-derived cardiomyocytes: response to TTX and lidocain reveals strong cell to cell variability
    Xiaowu Sheng
    Institute for Neurophysiology, University of Cologne, Germany
    PLoS ONE 7:e45963. 2012
  3. pmc Initial colony morphology-based selection for iPS cells derived from adult fibroblasts is substantially improved by temporary UTF1-based selection
    Kurt Pfannkuche
    Center of Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Cologne, Germany
    PLoS ONE 5:e9580. 2010
  4. doi request reprint The high mobility group protein HMGA2: a co-regulator of chromatin structure and pluripotency in stem cells?
    Kurt Pfannkuche
    Institute for Neurophysiology, University of Cologne, Robert Koch Str 39, 50931, Cologne, Germany
    Stem Cell Rev 5:224-30. 2009
  5. doi request reprint Fibroblasts support functional integration of purified embryonic stem cell-derived cardiomyocytes into avital myocardial tissue
    Jiaoya Xi
    Institute for Neurophysiology, University of Cologne, Cologne, Germany
    Stem Cells Dev 20:821-30. 2011
  6. pmc The L-type Ca2+ channels blocker nifedipine represses mesodermal fate determination in murine embryonic stem cells
    Filomain Nguemo
    Institute of Neurophysiology, University of Cologne, Cologne, Germany
    PLoS ONE 8:e53407. 2013
  7. ncbi request reprint Physiological differences between transplanted and host tissue cause functional decoupling after in vitro transplantation of human embryonic stem cell-derived cardiomyocytes
    Frank Pillekamp
    Pediatric Cardiology, University of Cologne, Cologne, Germany
    Cell Physiol Biochem 23:65-74. 2009
  8. doi request reprint Comparison of contractile behavior of native murine ventricular tissue and cardiomyocytes derived from embryonic or induced pluripotent stem cells
    Jiaoya Xi
    Institute for Neurophysiology, University of Cologne, Cologne, Germany
    FASEB J 24:2739-51. 2010
  9. doi request reprint Cardiac myocytes derived from murine reprogrammed fibroblasts: intact hormonal regulation, cardiac ion channel expression and development of contractility
    Kurt Pfannkuche
    Institute for Neurophysiology, University of Cologne, Germany
    Cell Physiol Biochem 24:73-86. 2009
  10. ncbi request reprint Isolation and functional characterization of alpha-smooth muscle actin expressing cardiomyocytes from embryonic stem cells
    Shiva Prasad Potta
    Center of Physiology and Pathophysiology, Institute of Neurophysiology, and Center of Molecular Medicine, University of Cologne CMMC, Cologne, Germany
    Cell Physiol Biochem 25:595-604. 2010

Collaborators

Detail Information

Publications30

  1. doi request reprint Induced pluripotent stem cells: a new approach for physiological research
    Kurt Pfannkuche
    Institute for Neurophysiology, University of Cologne, Germany
    Cell Physiol Biochem 26:105-24. 2010
    ..We compare ES and iPS cells to answer the question whether these cells are identical, and we finish with an outlook on clinical research with iPS cells with a focus on cardiovascular medicine...
  2. pmc Human pluripotent stem cell-derived cardiomyocytes: response to TTX and lidocain reveals strong cell to cell variability
    Xiaowu Sheng
    Institute for Neurophysiology, University of Cologne, Germany
    PLoS ONE 7:e45963. 2012
    ..These data point to physiological differences that are not necessarily detected by genomics. We conclude that novel pharmacological screening-assays using hiPSC-CMs need to be applied with some caution...
  3. pmc Initial colony morphology-based selection for iPS cells derived from adult fibroblasts is substantially improved by temporary UTF1-based selection
    Kurt Pfannkuche
    Center of Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Cologne, Germany
    PLoS ONE 5:e9580. 2010
    ..Recently, selection of fully reprogrammed cells was achieved based on colony morphology reminiscent of embryonic stem (ES) cells. The maintenance of pluripotency was analysed...
  4. doi request reprint The high mobility group protein HMGA2: a co-regulator of chromatin structure and pluripotency in stem cells?
    Kurt Pfannkuche
    Institute for Neurophysiology, University of Cologne, Robert Koch Str 39, 50931, Cologne, Germany
    Stem Cell Rev 5:224-30. 2009
    ....
  5. doi request reprint Fibroblasts support functional integration of purified embryonic stem cell-derived cardiomyocytes into avital myocardial tissue
    Jiaoya Xi
    Institute for Neurophysiology, University of Cologne, Cologne, Germany
    Stem Cells Dev 20:821-30. 2011
    ..We conclude that noncardiomyocytes like MEFs support morphological integration and force transmission of purified ES-CMs by enabling adhesion and migration...
  6. pmc The L-type Ca2+ channels blocker nifedipine represses mesodermal fate determination in murine embryonic stem cells
    Filomain Nguemo
    Institute of Neurophysiology, University of Cologne, Cologne, Germany
    PLoS ONE 8:e53407. 2013
    ....
  7. ncbi request reprint Physiological differences between transplanted and host tissue cause functional decoupling after in vitro transplantation of human embryonic stem cell-derived cardiomyocytes
    Frank Pillekamp
    Pediatric Cardiology, University of Cologne, Cologne, Germany
    Cell Physiol Biochem 23:65-74. 2009
    ..Successful cellular replacement has to improve coupling but should also aim to transplant cardiomyocytes with similar electrophysiological properties as the host tissue...
  8. doi request reprint Comparison of contractile behavior of native murine ventricular tissue and cardiomyocytes derived from embryonic or induced pluripotent stem cells
    Jiaoya Xi
    Institute for Neurophysiology, University of Cologne, Cologne, Germany
    FASEB J 24:2739-51. 2010
    ..The results indicate immaturity of the sarcoplasmic reticulum and the beta-adrenergic response of iPS-CMs and ESC-CMs...
  9. doi request reprint Cardiac myocytes derived from murine reprogrammed fibroblasts: intact hormonal regulation, cardiac ion channel expression and development of contractility
    Kurt Pfannkuche
    Institute for Neurophysiology, University of Cologne, Germany
    Cell Physiol Biochem 24:73-86. 2009
    ..Here we aimed at generating iPS cell-derived cardiomyocytes (CMs) and comparing their molecular and functional characteristics with CMs derived from native murine ES cells...
  10. ncbi request reprint Isolation and functional characterization of alpha-smooth muscle actin expressing cardiomyocytes from embryonic stem cells
    Shiva Prasad Potta
    Center of Physiology and Pathophysiology, Institute of Neurophysiology, and Center of Molecular Medicine, University of Cologne CMMC, Cologne, Germany
    Cell Physiol Biochem 25:595-604. 2010
    ..The findings of the present study provide a novel approach for the identification and enrichment of Acta2-positive cardiomyocytes, especially of the ventricular phenotype under in vitro conditions...
  11. ncbi request reprint Neonatal murine heart slices. A robust model to study ventricular isometric contractions
    Frank Pillekamp
    Pediatric Cardiology, University of Cologne, Cologne, Germany
    Cell Physiol Biochem 20:837-46. 2007
    ..However, current multicellular preparations to investigate contractile properties have substantial technical and biological limitations and are especially difficult to apply to the developing murine heart...
  12. pmc Global transcriptional profiles of beating clusters derived from human induced pluripotent stem cells and embryonic stem cells are highly similar
    Manoj K Gupta
    Center for Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Robert Koch Str 39, 50931 Cologne, Germany
    BMC Dev Biol 10:98. 2010
    ..In this study we compared global transcriptomes of beating clusters (BCs) microdissected from differentiating human iPS cells and embryonic stem (ES) cells...
  13. doi request reprint A Cre-based double fluorescence indicator system for monitoring cell fusion events and selection of fused cells
    Kurt Pfannkuche
    Institute for Neurophysiology, University of Cologne, Cologne, Germany
    Biotechniques 48:113-20. 2010
    ..Here we have established a new methodology that not only allows the monitoring of the transport of cellular contents, but also enables the purification of fused cells using puromycin...
  14. pmc Evidence for a critical role of catecholamines for cardiomyocyte lineage commitment in murine embryonic stem cells
    Martin Lehmann
    Institute for Neurophysiology, University of Cologne, Cologne, Germany
    PLoS ONE 8:e70913. 2013
    ..Using several methodological approaches our data suggest that reserpine inhibits cardiac differentiation. Thus catecholamines play a critical role during development. ..
  15. doi request reprint Time-course of the electrophysiological maturation and integration of transplanted cardiomyocytes
    Marcel Halbach
    Department of Internal Medicine III, University of Cologne, Kerpener Str 62, 50937 Cologne, Germany
    J Mol Cell Cardiol 53:401-8. 2012
    ..The pace of maturation correlates with the quality of electrical integration. Transplanted cells embedded in cryoinjured tissue still possess immature electrophysiological properties after 12 days...
  16. doi request reprint Functional characterization of cardiomyocytes derived from murine induced pluripotent stem cells in vitro
    Alexey Kuzmenkin
    Institute for Neurophysiology, Medical Center, University of Cologne, Cologne, Germany
    FASEB J 23:4168-80. 2009
    ..This difference may reflect the incomplete reprogramming of iPS cells and should be critically considered in further studies to clarify the suitability of the iPS model for regenerative medicine of heart disorders...
  17. doi request reprint Functional characterization and transcriptome analysis of embryonic stem cell-derived contractile smooth muscle cells
    Shiva Prasad Potta
    University of Cologne, Center of Physiology and Pathophysiology, Institute of Neurophysiology, Robert Koch Str 39, Cologne, Germany
    Hypertension 53:196-204. 2009
    ....
  18. ncbi request reprint Electrophysiological maturation and integration of murine fetal cardiomyocytes after transplantation
    Marcel Halbach
    Department III of Internal Medicine, University of Cologne, Cologne, Germany
    Circ Res 101:484-92. 2007
    ..In conclusion, electrophysiological maturation and integration of transplanted FCMs depend on an embedment in viable host myocardium. FCMs surrounded by cryoinjured tissue maintain physiological but immature AP properties...
  19. doi request reprint Direct contact of umbilical cord blood endothelial progenitors with living cardiac tissue is a requirement for vascular tube-like structures formation
    Marilena Lupu
    Institute of Cellular Biology and Pathology Nicolae Simionescu, Bucharest, Romania Institute for Neurophysiology, University of Cologne, Cologne, Germany
    J Cell Mol Med 15:1914-26. 2011
    ..Understanding the cardiac niche and micro-environmental interactions that regulate EPCs integration and neovascularization are essential for applying these cells to cardiovascular regeneration...
  20. doi request reprint Fibroblasts facilitate the engraftment of embryonic stem cell-derived cardiomyocytes on three-dimensional collagen matrices and aggregation in hanging drops
    Kurt Pfannkuche
    Institute for Neurophysiology, University of Cologne, Cologne, Germany
    Stem Cells Dev 19:1589-99. 2010
    ..We conclude that fibroblasts promote cardiomyocyte engraftment and formation of functional 3-dimensional tissue in vitro. Elucidation of the mechanism of this phenomenon may help improve the integration of cardiomyocytes in vivo...
  21. ncbi request reprint Functional Characterization and Gene Expression Profiling of α-Smooth Muscle Actin Expressing Cardiomyocytes Derived from Murine Induced Pluripotent Stem Cells
    Shiva Prasad Potta
    Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Robert Koch Str 39, 50931, Cologne, Germany
    Stem Cell Rev 8:229-42. 2012
  22. doi request reprint In vitro model for assessing arrhythmogenic properties of drugs based on high-resolution impedance measurements
    Filomain Nguemo
    Institute of Neurophysiology, University of Cologne, Cologne, Germany
    Cell Physiol Biochem 29:819-32. 2012
    ..Moreover, methods for analyzing beating behavior of cardiomyocytes (CMs) in culture to diagnose arrhythmias are not well developed...
  23. ncbi request reprint Cell biological effects of mechanical stimulations generated by focused extracorporeal shock wave applications on cultured human bone marrow stromal cells
    Frank Suhr
    Institute of Cardiovascular Research and Sport Medicine, Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, Cologne, Germany
    Stem Cell Res 11:951-64. 2013
    ..Therefore, this approach opens the door for exploiting the full potential of these cells in regenerative medicine. ..
  24. ncbi request reprint The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes
    Carlos O Heras-Bautista
    Center for Physiology and Pathophysiology, Institute for Neurophysiology, University of Cologne, Cologne, Germany
    Biomaterials 35:7374-85. 2014
    ....
  25. pmc Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes
    Michael Xavier Doss
    Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Cologne, Germany
    Genome Biol 8:R56. 2007
    ..Characterization of gene expression signatures for cardiomyocytes derived from embryonic stem cells will help to define their early biologic processes...
  26. doi request reprint Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events
    Kurt Pfannkuche
    Institute for Neurophysiology, Robert Koch Str 39, 50931 Cologne, Germany
    Nat Protoc 3:1510-26. 2008
    ..Ultimately, this powerful methodology facilitates Cre/loxP-based applications such as cell lineage tracking or monitoring of cell fusion...
  27. pmc Mesenchymal stem cells and their conditioned medium improve integration of purified induced pluripotent stem cell-derived cardiomyocyte clusters into myocardial tissue
    Martin Rubach
    1 Department of Pediatric Cardiology, University of Cologne, Cologne, Germany
    Stem Cells Dev 23:643-53. 2014
    ..These data suggest that MSCs may increase the functional engraftment and therapeutic efficacy of transplanted iPS-CMs into infarcted myocardium. ..
  28. pmc Intense resistance exercise induces early and transient increases in ryanodine receptor 1 phosphorylation in human skeletal muscle
    Sebastian Gehlert
    Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, Germany
    PLoS ONE 7:e49326. 2012
    ..However, no data are available regarding the time-course and magnitude of early RyR1 and AMPK phosphorylation in human myofibers in response to acute resistance exercise...
  29. pmc Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: a tool for efficient genetic engineering of mammalian genomes
    Michael Peitz
    Institute for Genetics, University of Cologne, Weyertal 121, 50931 Cologne, Germany
    Proc Natl Acad Sci U S A 99:4489-94. 2002
    ..We expect that application of His-TAT-NLS-Cre, which can be produced readily in large quantities from a bacterial source, will expand our abilities to manipulate mammalian genomes...
  30. ncbi request reprint Force measurements of human embryonic stem cell-derived cardiomyocytes in an in vitro transplantation model
    Frank Pillekamp
    Department of Pediatric Cardiology, University of Cologne, Cologne, Germany
    Stem Cells 25:174-80. 2007
    ..Our data demonstrate that this novel in vitro transplantation model is well suited to assess the mechanical properties and functional integration of cells suggested for cardiac replacement strategies...