J Oldenburg

Summary

Affiliation: University of Bonn
Country: Germany

Publications

  1. doi request reprint Tr1 and naturally occurring regulatory T cells induce IgG4 in B cells through GITR/GITR-L interaction, IL-10 and TGF-beta
    Judith S Satoguina
    Institute of Medical Microbiology, Immunology and Parasitology, University Clinic Bonn, Bonn, Germany
    Eur J Immunol 38:3101-13. 2008
  2. ncbi request reprint Comparative genetics of warfarin resistance
    J Oldenburg
    Prof Dr Johannes Oldenburg, Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany, E mail Tel 49 0 228 287 51 75, Fax 49 0 228 287 51 76
    Hamostaseologie 34:143-59. 2014
  3. ncbi request reprint Haemophilia A mutations in patients with non-severe phenotype associated with a discrepancy between one-stage and chromogenic factor VIII activity assays
    Anna Pavlova
    Prof Dr Johannes Oldenburg, Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany, Tel 49 228 287 15175, Fax 49 228 287 14783, E mail
    Thromb Haemost 111:851-61. 2014
  4. doi request reprint Effect of F8 B domain gene variants on synthesis, secretion, activity and stability of factor VIII protein
    Saskia Pahl
    Prof Dr Johannes Oldenburg, Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, Germany, Tel 49 228 287 15175, Fax 49 228 287 14783, E mail
    Thromb Haemost 111:58-66. 2014
  5. pmc Insights into pathological mechanisms of missense mutations in C-terminal domains of von Willebrand factor causing qualitative or quantitative von Willebrand disease
    Hamideh Yadegari
    Institute of Experimental Haematology and Transfusion Medicine, University Clinics Bonn, Germany
    Haematologica 98:1315-23. 2013
  6. ncbi request reprint Mutation distribution in the von Willebrand factor gene related to the different von Willebrand disease (VWD) types in a cohort of VWD patients
    Hamideh Yadegari
    Institute of Experimental Haematology and Transfusion Medicine, University Clinics Bonn, Germany
    Thromb Haemost 108:662-71. 2012
  7. doi request reprint Deficiencies of antithrombin, protein C and protein S - practical experience in genetic analysis of a large patient cohort
    Michael Caspers
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Thromb Haemost 108:247-57. 2012
  8. pmc Prediction of phenprocoumon maintenance dose and phenprocoumon plasma concentration by genetic and non-genetic parameters
    Christof Geisen
    German Red Cross, Institute of Transfusion Medicine and Immunohaematology, University Hospital Frankfurt, Frankfurt, Germany
    Eur J Clin Pharmacol 67:371-81. 2011
  9. pmc Association of COMT genotypes with S-COMT promoter methylation in growth-discordant monozygotic twins and healthy adults
    Felix Schreiner
    Pediatric Endocrinology Division, Children s Hospital, University of Bonn, Adenauerallee 119, Bonn 53113, Germany
    BMC Med Genet 12:115. 2011
  10. pmc Novel mutations in the VKORC1 gene of wild rats and mice--a response to 50 years of selection pressure by warfarin?
    Simone Rost
    Department of Human Genetics, University of Wuerzburg, Wuerzburg, Germany
    BMC Genet 10:4. 2009

Detail Information

Publications140 found, 100 shown here

  1. doi request reprint Tr1 and naturally occurring regulatory T cells induce IgG4 in B cells through GITR/GITR-L interaction, IL-10 and TGF-beta
    Judith S Satoguina
    Institute of Medical Microbiology, Immunology and Parasitology, University Clinic Bonn, Bonn, Germany
    Eur J Immunol 38:3101-13. 2008
    ..Thus, the mechanism of IgG4 induction by regulatory cells involves GITR-GITR-L interactions, IL-10 and TGF-beta...
  2. ncbi request reprint Comparative genetics of warfarin resistance
    J Oldenburg
    Prof Dr Johannes Oldenburg, Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany, E mail Tel 49 0 228 287 51 75, Fax 49 0 228 287 51 76
    Hamostaseologie 34:143-59. 2014
    ....
  3. ncbi request reprint Haemophilia A mutations in patients with non-severe phenotype associated with a discrepancy between one-stage and chromogenic factor VIII activity assays
    Anna Pavlova
    Prof Dr Johannes Oldenburg, Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany, Tel 49 228 287 15175, Fax 49 228 287 14783, E mail
    Thromb Haemost 111:851-61. 2014
    ..Therefore, we recommend that initial diagnosis of non-severe HA phenotypes should be based on results of both FVIII:C1st and FVIII:Cchr assays. ..
  4. doi request reprint Effect of F8 B domain gene variants on synthesis, secretion, activity and stability of factor VIII protein
    Saskia Pahl
    Prof Dr Johannes Oldenburg, Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, Germany, Tel 49 228 287 15175, Fax 49 228 287 14783, E mail
    Thromb Haemost 111:58-66. 2014
    ..Nevertheless, the mutations p.Asp845Glu, p.Pro947Arg, p.Glu1057Lys, p.His1066Tyr, p.Arg1126Trp, p.Arg1329His, p.Leu1481Pro, and p.Ala1610Ser resulted in decreased FVIII:C values that may explain mild HA phenotypes. ..
  5. pmc Insights into pathological mechanisms of missense mutations in C-terminal domains of von Willebrand factor causing qualitative or quantitative von Willebrand disease
    Hamideh Yadegari
    Institute of Experimental Haematology and Transfusion Medicine, University Clinics Bonn, Germany
    Haematologica 98:1315-23. 2013
    ..The results highlight the importance of cysteine residues within the carboxyl-terminal of von Willebrand factor on structural conformation of the protein and consequently multimerization, storage, and secretion of von Willebrand factor. ..
  6. ncbi request reprint Mutation distribution in the von Willebrand factor gene related to the different von Willebrand disease (VWD) types in a cohort of VWD patients
    Hamideh Yadegari
    Institute of Experimental Haematology and Transfusion Medicine, University Clinics Bonn, Germany
    Thromb Haemost 108:662-71. 2012
    ..Genotyping in VWD is a helpful tool to further elucidate the pathogenesis of VWD and to establish the relationship between genotype and phenotype...
  7. doi request reprint Deficiencies of antithrombin, protein C and protein S - practical experience in genetic analysis of a large patient cohort
    Michael Caspers
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Thromb Haemost 108:247-57. 2012
    ..Based on our data, genetic analysis for patients with AT deficiency is indicated for all subnormal activities. In contrast, genotyping is not advisable for PC activities >70% and for PS activities >55%...
  8. pmc Prediction of phenprocoumon maintenance dose and phenprocoumon plasma concentration by genetic and non-genetic parameters
    Christof Geisen
    German Red Cross, Institute of Transfusion Medicine and Immunohaematology, University Hospital Frankfurt, Frankfurt, Germany
    Eur J Clin Pharmacol 67:371-81. 2011
    ..The impact of single nucleotide polymorphisms (SNPs) in several genes of enzymes involved in the vitamin K cycle on phenprocoumon dose variability and phenprocoumon plasma concentrations is still under investigation...
  9. pmc Association of COMT genotypes with S-COMT promoter methylation in growth-discordant monozygotic twins and healthy adults
    Felix Schreiner
    Pediatric Endocrinology Division, Children s Hospital, University of Bonn, Adenauerallee 119, Bonn 53113, Germany
    BMC Med Genet 12:115. 2011
    ..So far, studies investigating the epigenetic variability of the S-COMT (soluble COMT) promoter region mainly focused on phenotypical aspects, and results have been controversial...
  10. pmc Novel mutations in the VKORC1 gene of wild rats and mice--a response to 50 years of selection pressure by warfarin?
    Simone Rost
    Department of Human Genetics, University of Wuerzburg, Wuerzburg, Germany
    BMC Genet 10:4. 2009
    ..Resistance to these compounds has been reported for rodent populations from many countries around the world and poses a considerable problem for efficacy of pest control...
  11. ncbi request reprint VKORC1: molecular target of coumarins
    J Oldenburg
    Institute for Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    J Thromb Haemost 5:1-6. 2007
    ..Thus, co-expression of VKORC1 leads to a more efficient production of recombinant vitamin K-dependent coagulation factors such as FIX and FVII. This could improve production of recombinant factor concentrates in the future...
  12. ncbi request reprint Discrepancy between one-stage and chromogenic factor VIII activity assay results can lead to misdiagnosis of haemophilia A phenotype
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Hamostaseologie 30:207-11. 2010
    ..The current understanding of the mechanistic basis for how FVIII:C assay discrepancies arise are discussed...
  13. ncbi request reprint Genetic risk factors for inhibitors to factors VIII and IX
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 12:15-22. 2006
    ..Improved understanding of these complex interactions may lead to the development of preventive measures to minimize inhibitor formation...
  14. doi request reprint Postauthorization safety surveillance of ADVATE [antihaemophilic factor (recombinant), plasma/albumin-free method] demonstrates efficacy, safety and low-risk for immunogenicity in routine clinical practice
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 16:866-77. 2010
    ..The favourable benefit/risk profile of rAHF-PFM previously documented in prospective clinical trials has been extended to include a broader range of haemophilia patients, many of whom would have been ineligible for registration studies...
  15. doi request reprint Prophylaxis in bleeding disorders
    Johannes Oldenburg
    Institute for Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Thromb Res 127:S14-7. 2011
    ..Ongoing studies will help further define the role of coagulation factor concentrate prophylaxis in patients with bleeding disorders...
  16. ncbi request reprint Vitamin K epoxide reductase complex subunit 1 (VKORC1): the key protein of the vitamin K cycle
    Johannes Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Antioxid Redox Signal 8:347-53. 2006
    ....
  17. ncbi request reprint New insight into the molecular basis of hemophilia A
    Johannes Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, Bonn, Germany
    Int J Hematol 83:96-102. 2006
    ..Moreover, the proof of an absence of F8 messenger RNA (mRNA) in one patient points to either a defect in the expression of F8 mRNA or its rapid degradation, which may represent a novel mechanism leading to HA...
  18. ncbi request reprint [Blood coagulation and hemorrhagic diathesis]
    Johannes Oldenburg
    Institut für Experimentelle Hämatologie und Transfusionsmedizin des Universitätsklinikums Bonn
    Pharm Unserer Zeit 35:20-8. 2006
  19. ncbi request reprint Current pharmacogenetic developments in oral anticoagulation therapy: the influence of variant VKORC1 and CYP2C9 alleles
    Johannes Oldenburg
    Institute for Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, D 53105 Bonn, Germany
    Thromb Haemost 98:570-8. 2007
    ..The use of new pharmacogenetics-based dosing schemes and the concomitant application of low-dose vitamin K with coumarins will decidedly influence the current practice of oral anticoagulation and greatly improve coumarin drug safety...
  20. ncbi request reprint [Current and future prospects in haemostasis]
    J Oldenburg
    Institut für Experimentelle Hämatologie und Transfusionsmedizin, Universitätsklinik Bonn, Sigmund Freud Str 25, 53105 Bonn
    Hamostaseologie 26:13-21. 2006
    ..Patients will profit from these developments by individualised prevention and therapy regimens, including future approaches of regenerative medicine as stem cell and gene therapy...
  21. ncbi request reprint Molecular biology of blood coagulation
    J Oldenburg
    Institute for Experimental Hematology and Transfusion Services, University Clinics, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Semin Thromb Hemost 27:313-24. 2001
    ..The aim of this article is to illustrate this progress by reporting on the recent results in representative hereditary hemorrhagic and such thromboembolic conditions as hemophilia, von Willebrand disease, and thrombotic disorders...
  22. ncbi request reprint [Congenital coagulopathies and coagulation factor inhibitors]
    J Oldenburg
    Institut für Experimentelle Hämatologie und Transfusionsmedizin des Universitätsklinikums Bonn
    Hamostaseologie 28:335-47. 2008
    ..The Guidelines of the Federal Chamber of Physicians, the German recommendations for ITT and the consensus recommendations of an international working group provide an important lead for the conduction of immune tolerance therapy...
  23. doi request reprint Haemophilia care then, now and in the future
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 15:2-7. 2009
    ..To set the scene for the following papers dealing with haemophilia care from paediatrics to geriatrics, developments behind these improvements and some aspects of future research will be presented in this paper...
  24. doi request reprint Genetic markers in acquired haemophilia
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 16:41-5. 2010
    ..17). This observation was mainly because of a higher frequency of the CTLA-4 + 49 G allele in female patients. These findings suggest that immune response genes may contribute to the development of anti-factor VIII autoantibodies in AH...
  25. ncbi request reprint [Immune tolerance therapy for inhibitors in haemophilia A]
    J Oldenburg
    Institute of Experimental Haematology, and Transfusion Medicine University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Hamostaseologie 28:S23-5. 2008
    ....
  26. doi request reprint Thirteen novel VKORC1 mutations associated with oral anticoagulant resistance: insights into improved patient diagnosis and treatment
    M Watzka
    Institute of Experimental Hematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    J Thromb Haemost 9:109-18. 2011
    ..Vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1) is the molecular target of oral anticoagulants. Mutations in VKORC1 cause partial or total coumarin resistance...
  27. ncbi request reprint AHEAD. Advate in HaEmophilia A outcome Database
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic, Sigmund Freud Str 25, 53127 Bonn, Germany
    Hamostaseologie 30:S23-5. 2010
    ..The first patient was enrolled in the study in early June 2010; recruitment is planned to continue until the end of 2011. The Ethics Committee of the University of Bonn has given its favorable opinion...
  28. ncbi request reprint [New insight in therapeutic anticoagulation by Coumarin derivatives]
    J Oldenburg
    Institute of Experimental Haematology, and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Hamostaseologie 28:44-50. 2008
    ..The well established and oeconomic coumarin drugs will benefit from a pharmacogenetic and nutritive adjusted optimization of therapy...
  29. ncbi request reprint HLA genotype in patients with acquired haemophilia A
    A Pavlova
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 16:107-12. 2010
    ..It might be speculated that the presence or absence of the FVIII antigen and the various ability of HLA molecules to present the FVIII antigen to the T-cell receptor contribute to these findings...
  30. pmc Progression of liver fibrosis in HIV/HCV genotype 1 co-infected patients is related to the T allele of the rs12979860 polymorphism of the IL28B gene
    P Lutz
    Department of Internal Medicine I, University Hospital Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany
    Eur J Med Res 16:335-41. 2011
    ..Our aim was to clarify by non-invasive techniques if this polymorphism affects fibrosis progression in HIV/HCV co-infection...
  31. ncbi request reprint Mutations affecting disulphide bonds contribute to a fairly common prevalence of F13B gene defects: results of a genetic study in 14 families with factor XIII B deficiency
    V Ivaskevicius
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 16:675-82. 2010
    ..The present study reports on a fairly common prevalence of F13B gene defects in the German population. The regions in and around the cysteine disulphide bonds in the FXIII-B protein may be regions prone to frequent mutations...
  32. ncbi request reprint [Pseudo tumours in haemophilia patients]
    P Berdel
    Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, Sigmund Freud Strasse 25, 53127 Bonn
    Hamostaseologie 29:S74-6. 2009
    ..This results in bone resorption and destruction of surrounding muscular and soft tissue. Pseudo tumours develop slowly over many years. They occur primarily in adults and are largely unresponsive to conservative treatment...
  33. ncbi request reprint Impact of polymorphisms of the major histocompatibility complex class II, interleukin-10, tumor necrosis factor-alpha and cytotoxic T-lymphocyte antigen-4 genes on inhibitor development in severe hemophilia A
    A Pavlova
    Experimental Heamatology and Transfusion Medicine, University of Clinic Bonn, Sigmund Freud Str 25, Germany
    J Thromb Haemost 7:2006-2015. 2009
    ....
  34. doi request reprint Identification of a third rearrangement at Xq28 that causes severe hemophilia A as a result of homologous recombination between inverted repeats
    B Pezeshkpoor
    Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
    J Thromb Haemost 10:1600-8. 2012
    ....
  35. ncbi request reprint Analysis of mRNA in hemophilia A patients with undetectable mutations reveals normal splicing in the factor VIII gene
    O El-Maarri
    Institute of Experimental Haematology and Transfusion Medicine, Bonn, Germany
    J Thromb Haemost 3:332-9. 2005
    ....
  36. ncbi request reprint Heterozygous large deletions of Factor 8 gene in females identified by multiplex PCR-LC
    A Pavlova
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 14:599-606. 2008
    ..In conclusion, the multiplex PCR-LC technique represents a rapid, simple and reliable method for detection of heterozygous large deletions in female carriers...
  37. ncbi request reprint The relevance of the bleeding severity in the treatment of acquired haemophilia - an update of a single-centre experience with 67 patients
    H Zeitler
    Internal Medical Clinic I of the University of Bonn, Centre of Extracorporeal Therapy and Autoimmunity CETA, Bonn, Germany
    Haemophilia 16:95-101. 2010
    ..Treatment procedures should be adapted to bleeding severity and inhibitor titres. Under these conditions, AH is a potentially curable autoimmune disorder with an excellent prognosis...
  38. pmc Transient elastography discloses identical distribution of liver fibrosis in chronic hepatitis C between HIV-negative and HIV-positive patients on HAART
    F Grunhage
    Department of Internal Medicine I, University Hospital Bonn, Bonn, Germany
    Eur J Med Res 15:139-44. 2010
    ..Highly active antiretroviral therapy (HAART) efficiently restores and preserves immune functions and has recently been demonstrated to also result in reduced liver-related mortality in HIV/HCV-co-infected patients...
  39. ncbi request reprint Increased frequency of the CTLA-4 49 A/G polymorphism in patients with acquired haemophilia A compared to healthy controls
    A Pavlova
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 14:355-60. 2008
    ..In conclusion, on the background of the CTLA-4 gene polymorphism, further genetic and/or environmental factors might contribute to and finally trigger the clinical manifestation of AH...
  40. pmc Somatic mosaicism in hemophilia A: a fairly common event
    M Leuer
    Department of Clinical Biochemistry, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Am J Hum Genet 69:75-87. 2001
    ..As a consequence, risk assessment in genetic counseling should include consideration of the possibility of somatic mosaicism in families with apparently de novo mutations, especially families with the subtype of point mutations...
  41. ncbi request reprint [Upper ankle joint prostheses in haemophilia patients]
    P Berdel
    Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, Sigmund Freud Strasse 25, 53127 Bonn
    Hamostaseologie 29:S65-8. 2009
    ..In such clinical situations, implantation of a subtalar joint endoprosthesis is a viable alternative to arthrodesis...
  42. ncbi request reprint Methylation analysis of the promoter region and intron 1 of the factor VIII gene in haemophilia A patients
    M A Zimmermann
    Melanie Andrea Zimmermann, Department of Human Genetics, University of Wurzburg, Biocenter, Am Hubland, 97074 Wurzburg, Germany, Tel 49 0 931 318 12 78 Fax 49 0 931 318 40 69, E mail
    Hamostaseologie 33:S46-9. 2013
    ..The methylation patterns of haemophilia A patients did not differ from that of controls. In three patients, chromosomal aberrations were identified which could be associated with a defective FVIII synthesis. ..
  43. ncbi request reprint Factor 5 mutation profile in German patients with homozygous and heterozygous factor V deficiency
    D Delev
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 15:1143-53. 2009
    ..Identifying the molecular basis of mutations underlying this rare coagulation disorder will allow more insight into the mechanisms involved in the variable clinical phenotypes of patients with FV deficiency...
  44. doi request reprint Association between phenotype and genotype in carriers of haemophilia A
    W Miesbach
    Medical Clinic III, Institute of Transfusion Medicine, Goethe University, Frankfurt, Germany
    Haemophilia 17:246-51. 2011
    ..Incidence and intensity of bleeding symptoms of haemophilia A carriers are high and correlated with the phenotype of the male haemophilic relative and the underlying F8 gene mutation...
  45. ncbi request reprint Induction of immune tolerance in haemophilia A inhibitor patients by the 'Bonn Protocol': predictive parameter for therapy duration and outcome
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Bonn, Germany
    Vox Sang 77:49-54. 1999
    ..Furthermore our data suggest, that ITT should be started at low inhibitor titres preferably with a high factor VIII dosage protocol...
  46. ncbi request reprint Genetic predisposition to bleeding during oral anticoagulant therapy: evidence for common founder mutations (FIXVal-10 and FIXThr-10) and an independent CpG hotspot mutation (FIXThr-10)
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Germany
    Thromb Haemost 85:454-7. 2001
    ..This strategy will provide a cost-effective and safe procedure to identify patients that carry the FIX variants. Moreover, such a strategy accumulates data about the prevalence of these FIX mutations in a given population...
  47. ncbi request reprint Functional promoter polymorphism in the VKORC1 gene is no major genetic determinant for coronary heart disease in Northern Germans
    Matthias Watzka
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn Sigmund Freud Str 25, 53127 Bonn, Germany
    Thromb Haemost 97:998-1002. 2007
    ....
  48. ncbi request reprint Maternal alleles acquiring paternal methylation patterns in biparental complete hydatidiform moles
    Osman El-Maarri
    Institute of Experimental Haematology and Transfusion Medicine, Bonn 53105, Germany
    Hum Mol Genet 12:1405-13. 2003
    ..The defective 19q13.4 locus may have led to the development of variable degrees of 'faulty' paternal marks on the maternal chromosomes...
  49. doi request reprint Monitoring of plasma levels of activated protein C using a clinically applicable oligonucleotide-based enzyme capture assay
    J Muller
    Institute for Experimental Haematology and Transfusion Medicine Clinic for Orthopedic Surgery, University Clinic Bonn Life and Medical Sciences Institute, University of Bonn, Bonn, Germany
    J Thromb Haemost 10:390-8. 2012
    ..Human-activated protein C (APC) is a serine protease with anticoagulant, anti-inflammatory and cytoprotective functions. This feature renders APC to be a promising vascular-inflammatory biomarker...
  50. doi request reprint In vitro characterization of recombinant factor VIII concentrates reveals significant differences in protein content, activity and thrombin activation profile
    S Pahl
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 19:392-8. 2013
    ..chromogenic assay for B and C). The variation in thrombin activation profiles may arise from differences in cell line-dependent posttranslational modifications of the various recombinant proteins...
  51. doi request reprint Deep intronic 'mutations' cause hemophilia A: application of next generation sequencing in patients without detectable mutation in F8 cDNA
    B Pezeshkpoor
    Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
    J Thromb Haemost 11:1679-87. 2013
    ..In the current study, we performed a systematic screening of genetic and non-genetic parameters associated with reduced FVIII:C levels in a group of mostly mild HA (only one moderate) patients with no detectable mutations in F8 cDNA...
  52. ncbi request reprint Inhibitor development in correlation to factor VIII genotypes
    J Oldenburg
    Institute of Transfusion Medicine and Immune Haematology of the DRK Blood Donor Service Hessen, Frankfurt, Germany
    Haemophilia 8:23-9. 2002
    ..However, in severely affected haemophilia A patients, the influence of patient genetics on inhibitor titre and epitope specificity has yet to be elucidated...
  53. ncbi request reprint Assay discrepancy in mild haemophilia A due to a factor VIII missense mutation (Asn694Ile) in a large Danish family
    R Schwaab
    Institute for Experimental Haematology and Transfusion Medicine, Bonn, Germany
    Br J Haematol 109:523-8. 2000
    ..However, the current study rules out the co-existence of either von Willebrand's disease or the presence of the Normandy variant of von Willebrand factor (type 2N)...
  54. ncbi request reprint Molecular mechanisms underlying hemophilia A phenotype in seven females
    A Pavlova
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    J Thromb Haemost 7:976-82. 2009
    ..Hemophilia A (HA) in females is a rare observation. Here we describe various genetic mechanisms that result in phenotypic expression of HA in seven females...
  55. ncbi request reprint Frequency of contamination of coagulation factor concentrates with novel human parvovirus PARV4
    B Schneider
    Institute of Virology, University of Bonn Medical Centre, Bonn, Germany
    Haemophilia 14:978-86. 2008
    ..Further studies are needed to determine whether or not PARV4 contamination of coagulation factors causes harm to the product recipients...
  56. ncbi request reprint Patients with familial biparental hydatidiform moles have normal methylation at imprinted genes
    Osman El-Maarri
    Institute of Experimental Haematology and Transfusion Medicine, Bonn, Germany
    Eur J Hum Genet 13:486-90. 2005
    ....
  57. ncbi request reprint Primary and rescue immune tolerance induction in children and adults: a multicentre international study with a VWF-containing plasma-derived FVIII concentrate
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 20:83-91. 2014
    ....
  58. ncbi request reprint Molecular basis of haemophilia A
    J Oldenburg
    Institute of Transfusion Medicine and Immunohaematology, DRK Blood Donation Service, Baden, Württemberg Hessen, D 60528 Frankfurt, Germany
    Haemophilia 10:133-9. 2004
    ..These findings raise hope for novel recombinant FVIII molecules with prolonged half-life that may improve therapies for haemophlia A...
  59. doi request reprint Thrombin inhibition profiles in healthy individuals and thrombophilic patients
    Heiko Rühl
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Thromb Haemost 107:848-53. 2012
    ..In contrast, plasma levels of antithrombin falling below 80% of normal markedly prolong the thrombin half-life...
  60. ncbi request reprint Phenotype-genotype correlation in eight Polish patients with inherited Factor XIII deficiency: identification of three novel mutations
    V Ivaskevicius
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, 53127 Bonn, Germany
    Haemophilia 13:649-57. 2007
    ..In conclusion, the severe phenotype with high incidence of intracranial bleeding and haemarthrosis was in accordance with laboratory findings on FXIII and with severe molecular defects of the F13A gene...
  61. doi request reprint Kinetic parameters of monoclonal antibodies ESH2, ESH4, ESH5, and ESH8 on coagulation factor VIII and their influence on factor VIII activity
    C Egler
    Center of Advanced European Studies and Research, Bonn, Germany
    J Mol Recognit 22:301-6. 2009
    ..The systematic assessment of these results should support FVIII interaction studies, and can provide data to rationally test peptides/mimotopes to remove or neutralize inhibitors of FVIII activity...
  62. ncbi request reprint Gene therapy of hemophilia
    R Schwaab
    Institute for Experimental Hematology and Transfusion Medicine, University Clinics, Sigmund Freud Str 25, 53105 Bonn, Germany
    Semin Thromb Hemost 27:417-24. 2001
    ..Encouraged by the results of preliminary experiments using preponderant mouse and canine models, three clinical phase I studies on hemophilia A and B patients have been initiated, one of which has been preliminarily reported successful...
  63. ncbi request reprint [Muscular compartment syndrome of the forearm in a haemophilia inhibitor patient]
    P Berdel
    Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Hamostaseologie 28:S45-9. 2008
    ..In this case the immune system produces antibodies to factor VIII or IX during substitution therapy of haemophilia A or B. These antibodies are directed against both, the substituted and the endogenous factors...
  64. ncbi request reprint Risk factors for inhibitor development in hemophilia A
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Germany
    Haematologica 85:7-13; discussion 13-4. 2000
    ..Fixing the genetic predisposition by gene analysis will be one important tool in the future to assess further parameters that might influence inhibitor formation...
  65. doi request reprint Correlation of transient elastography with APRI and FIB-4 in a cohort of patients with congenital bleeding disorders and HCV or HIV/HCV coinfection
    N Vidovic
    Institute for Experimental Haemostasiology and Transfusion Medicine, University Hospital Bonn, Bonn, Germany
    Haemophilia 16:778-85. 2010
    ..Furthermore, our data support previous studies that observed a favourable outcome in patients with HIV/HCV and a preserved immune function in times of highly active antiretroviral therapy...
  66. ncbi request reprint Evaluation of DHPLC in the analysis of hemophilia A
    J Oldenburg
    Department of Human Genetics, University of Wurzburg, Biozentrum, Am Hubland, D 97074, Wurzburg, Germany
    J Biochem Biophys Methods 47:39-51. 2001
    ..4%), dHPLC facilitated the detection of the disease-associated nucleotide alterations. From these findings we conclude that the dHPLC technology is a highly sensitive method well suited to the molecular analysis of hemophilia A...
  67. ncbi request reprint Lithuanian haemophilia A and B registry comprising phenotypic and genotypic data
    V Ivaskevicius
    Klaipeda Seamen s Hospital, Haemophilia Centre, Klaipeda, Lithuania
    Br J Haematol 112:1062-70. 2001
    ..The information provided by this registry will be helpful for monitoring the treatment of Lithuanian haemophilia patients and also for reliable genetic counselling of the affected families in the future...
  68. ncbi request reprint [Algorithm for the treatment of the haemophilic arthropathia of the upper ankle joint]
    P Berdel
    Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, Sigmund Freud Strase 25, 53127 Bonn
    Hamostaseologie 30:S93-6. 2010
    ..In order to minimize the complication rate, their treatment should be restricted to specially equipped interdisciplinary centers with adequately trained and experienced surgeons as well as haemostaseologists...
  69. ncbi request reprint [Flexion contracture in haemophilic knee arthropathy--10-year follow-up after hamstring release and dorsal capsulotomy]
    P H Pennekamp
    Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, and St Bernhard Hospital Kamp Lintfort
    Z Orthop Unfall 145:317-21. 2007
    ..The aim of this study was to evaluate the long term results of joint preserving surgery with hamstring release and dorsal capsulotomy for the treatment of therapy resistant knee flexion contracture in patients with severe haemophilia...
  70. ncbi request reprint International registry on factor XIII deficiency: a basis formed mostly on European data
    Vytautas Ivaskevicius
    Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, 53127 Bonn, German
    Thromb Haemost 97:914-21. 2007
    ..f13-database.de) will provide clinicians and scientists working on FXIII deficiency with a helpful tool to improve patient care and direct future studies towards better understanding and treatment of the disease...
  71. ncbi request reprint Subclinical hyperthyroidism seems not to have a significant impact on systemic anticoagulation in patients with coumarin therapy
    Jan Bucerius
    Department of Nuclear Medicine, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Thromb Haemost 100:803-9. 2008
    ..In conclusion, subclinical hyperthyroidism seems to have no significant impact on coagulation metabolism in patients receiving anticoagulation therapy...
  72. doi request reprint Effects of HCV co-infection on apoptosis of CD4+ T-cells in HIV-positive patients
    Christian Korner
    Department of Internal Medicine I, University of Bonn, Germany, Sigmund Freud Str 25, Bonn 53127, Germany
    Clin Sci (Lond) 116:861-70. 2009
    ..The results of the present study suggest that HCV co-infection sensitizes CD4+ T-cells towards apoptosis in untreated HIV-positive patients. However, this effect is rapidly lost under effective antiretroviral therapy...
  73. doi request reprint Core-specific adaptive regulatory T-cells in different outcomes of hepatitis C
    Bettina Langhans
    Department of Internal Medicine I, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Clin Sci (Lond) 119:97-109. 2010
    ..Thus HCV core-specific Treg-cells may contribute to the immunoregulatory balance in chronic hepatitis C...
  74. doi request reprint Association between anxiety and factors of coagulation and fibrinolysis
    Franziska Geiser
    Clinic for Psychosomatic Medicine and Psychotherapy, University of Bonn, Bonn, Germany
    Psychother Psychosom 77:377-83. 2008
    ..However, hemostasis function has not yet been studied in patients with clinically relevant anxiety disorders...
  75. ncbi request reprint The first case of combined coagulation factor V and coagulation factor VIII deficiency in Poland due to a novel p.Tyr135Asn missense mutation in the MCFD2 gene
    Vytautas Ivaskevicius
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Blood Coagul Fibrinolysis 19:531-534. 2008
    ..Tyr135Asn). This variant represents the third missense mutation found in the MCFD2 gene and most likely disrupts the MCFD2-LMAN1 interaction, thus leading to the disease phenotype...
  76. doi request reprint A systematic search for DNA methyltransferase polymorphisms reveals a rare DNMT3L variant associated with subtelomeric hypomethylation
    Osman El-Maarri
    Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
    Hum Mol Genet 18:1755-68. 2009
    ....
  77. ncbi request reprint Lack of F8 mRNA: a novel mechanism leading to hemophilia A
    Osman El-Maarri
    Institute of Experimental Hematology and Transfusion Medicine, Sigmund Freud Str 25, 53127 Bonn, Germany
    Blood 107:2759-65. 2006
    ..These findings strongly suggest that the cause of HA in this patient is either absence or rapid degradation of the F8 mRNA, which points to a novel mechanism leading to HA...
  78. pmc Identification of eight novel coagulation factor XIII subunit A mutations: implied consequences for structure and function
    Vytautas Ivaskevicius
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, 53127 Bonn, Germany
    Haematologica 95:956-62. 2010
    ..In most cases, the disease is caused by F13A gene mutations. Causative mutations associated with the F13B gene are rarer...
  79. ncbi request reprint Association of a polymorphism of the ACVRL1 gene with sporadic arteriovenous malformations of the central nervous system
    Matthias Simon
    Neurochirurgische Klinik and Institut für Klinische Biochemie, Universitatskliniken Bonn, Germany
    J Neurosurg 104:945-9. 2006
    ..The authors studied whether sequence variations in ACVRL1 or ENG are associated with the development of clinically sporadic arteriovenous dysplasias and aneurysms of the CNS...
  80. ncbi request reprint Gender specific differences in levels of DNA methylation at selected loci from human total blood: a tendency toward higher methylation levels in males
    Osman El-Maarri
    Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Sigmund Freud Str 25, 53127, Bonn, Germany
    Hum Genet 122:505-14. 2007
    ..This observed difference could be due to the process of X chromosome inactivation or merely to the presence of an additional X chromosome in female cells or could be a result of downstream effects of sex determination...
  81. ncbi request reprint Fifteen years of env C2V3C3 evolution in six individuals infected clonally with human immunodeficiency virus type 1
    Bernd Kupfer
    Institute for Medical Microbiology, Immunology, and Parasitology, University of Bonn, Bonn, Germany
    J Med Virol 79:1629-39. 2007
    ..These common changes within the V3 loop are likely to be enforced by HIV-1 specific immune response...
  82. pmc A rapid, quantitative, non-radioactive bisulfite-SNuPE- IP RP HPLC assay for methylation analysis at specific CpG sites
    Osman El-Maarri
    Institute of Experimental Haematology and Transfusion Medicine, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Nucleic Acids Res 30:e25. 2002
    ..The assay is linear, highly reproducible and several sites can be measured simultaneously in one reaction. It can be semi-automated and eliminates the need for cloning and sequencing of individual bisulfite PCR products...
  83. doi request reprint Immunoadsorption in the treatment of acquired haemophilia
    Heike Zeitler
    Internal Medical Clinic I of the University of Bonn, Centre of Extracorporeal Therapy and Autoimmunity, Germany
    Atheroscler Suppl 10:122-5. 2009
    ..However, the severity of bleeding, and therefore the cost-effectiveness of the approach, needs to be considered when initiating this treatment protocol...
  84. pmc Methylation at global LINE-1 repeats in human blood are affected by gender but not by age or natural hormone cycles
    Osman El-Maarri
    Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
    PLoS ONE 6:e16252. 2011
    ..In conclusion, neither natural cycle of hormones nor age has a detectable effect on the LINE-1 methylation in peripheral blood cells, while gender remains an important factor...
  85. ncbi request reprint F8 genetic analysis strategies when standard approaches fail
    B Pezeshkpoor
    Osman El Maarri Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany, Tel 49 0 228 28 71 67 37, Fax 49 0 228 28 71 43 20, E mail
    Hamostaseologie 34:167-73. 2014
    ..In this review, we present the experiences with molecular diagnosis of such cases and approaches to be applied for mutation negative patients. ..
  86. doi request reprint Significance of F8 missense mutations with respect to inhibitor formation
    Rainer Schwaab
    Institute of Experimental Haematology and Transfusion Medicine, University Clinics Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Thromb Haemost 109:464-70. 2013
    ..These differences have to be identified in assigning risk profiles to aid in choice of preventative treatments designed to prevent inhibitor formation...
  87. ncbi request reprint [Comprehensive Care Center Bonn from 1980 to 2009. Changes in the epidemiology and regional composition of the haemophilia population]
    I Besmens
    Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Germany
    Hamostaseologie 31:S4-S10. 2011
    ..Due to the existence of well established primary haemophilia care in CCCs in Germany, the trend for the recent years is that the proportion of young patients that choose haemophilia care providers closer to their homes is increasing...
  88. ncbi request reprint Modified expression of coagulation factor VIII by addition of a glycosylation site at the N terminus of the protein
    M A Srour
    Institute for Experimental Hematology and Transfusion Medicine, University Clinics Bonn, University of Bonn, Sigmund Freud Str 25, 53105, Bonn, Germany
    Ann Hematol 87:107-12. 2008
    ..Thus, glycosylation alone does not automatically lead to higher secretion rates, but must be in context to the normal structure of the FVIII protein...
  89. ncbi request reprint Quality management and quality assurance in haemophilia care: a model at the Bonn haemophilia centre
    H H Brackmann
    Institut für Experimentelle Hämatologie und Transfusionsmedizin der Universität Bonn, Bonn, Germany
    Haemophilia 8:211-6. 2002
    ..All these and more are double-checked and interactive, controlling data and activities with the help of EDP. Exceptional staff motivation and patient compliance are important for this quality system...
  90. pmc Cancer risk in HIV-infected individuals on HAART is largely attributed to oncogenic infections and state of immunocompetence
    M Vogel
    Department of Internal Medicine I, Bonn University, Sigmund Freud Str 25, 53105 Bonn, Germany
    Eur J Med Res 16:101-7. 2011
    ..To estimate the cancer risk of HIV-infected patients in the HAART era with respect to a general reference population and to determine risk factors for malignancy...
  91. ncbi request reprint Effects of the CCR5-Delta32 mutation on hepatitis C virus-specific immune responses in patients with haemophilia
    Golo Ahlenstiel
    Department of Internal Medicine I, University of Bonn, Bonn, Germany
    Immunol Invest 38:284-96. 2009
    ..Interruption of the CCR5 signalling pathway due to CCR5-Delta32 may potentially result in subtle reduction of HCV specific IFN-gamma responses in anti-HCV-positive haemophiliac patients...
  92. doi request reprint The vitamin K cycle
    Johannes Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, D 53105 Bonn, Germany
    Vitam Horm 78:35-62. 2008
    ....
  93. ncbi request reprint Modelling and expression studies of two novel mutations causing factor V deficiency
    Daniel Delev
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Thromb Haemost 100:766-72. 2008
    ..Thus, both the data from the short duration molecular dynamic simulation and from expression analysis indicate alterations of the FV protein variants that explain the clinical phenotype...
  94. ncbi request reprint Coagulation factor XIII deficiency. Diagnosis, prevalence and management of inherited and acquired forms
    A Biswas
    Arijit Biswas Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25 53127 Bonn, Germany, Tel 49 0 228 28 71 94 28, Fax 49 0 228 28 71 43 20, E mail
    Hamostaseologie 34:160-6. 2014
    ..Here, we update the knowledge about the pathophysiology of factor XIII deficiency and its therapeutic options. ..
  95. doi request reprint The coagulation system of extremely preterm infants: influence of perinatal risk factors on coagulation
    C Poralla
    Department of Neonatology, University of Bonn, Bonn, Germany
    J Perinatol 32:869-73. 2012
    ..The objective of this study was to determine reference values for the components of the coagulation system at the first day of life in extremely preterm infants...
  96. ncbi request reprint Perioperative management and outcome of general and abdominal surgery in hemophiliacs
    Georg Goldmann
    Institute for Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
    Am J Surg 199:702-7. 2010
    ..The aim of the current study was to investigate perioperative management and outcome of surgery in hemophiliacs...
  97. ncbi request reprint Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography
    Jens Muller
    Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Sigmund Freud Str 25, 53127, Bonn, Germany
    Anal Bioanal Chem 390:1033-7. 2008
    ..We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences...
  98. ncbi request reprint Novel products for haemostasis - current status
    J Oldenburg
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Haemophilia 20:23-8. 2014
    ..Despite these challenges, the new products will significantly improve treatment and quality of life for our patients with haemophilia...
  99. ncbi request reprint 4beta-hydroxycholesterol as a marker of CYP3A4 inhibition in vivo - effects of itraconazole in man
    D Lutjohann
    Institute of Clinical Chemistry and Pharmacology, University Clinic, Bonn, Germany
    Int J Clin Pharmacol Ther 47:709-15. 2009
    ..Here, we study the potential endogenous serum marker of CYP3A4 activity, 4beta-hydroxycholesterol, during therapy with itraconazole...
  100. ncbi request reprint Rehabilitation of synovitis in patients with haemophilia
    A Seuser
    Orthopaedic Department, Kaiser Karl Klinik, Special Hospital for Rehabilitation Medicine, Bonn, Germany
    Haemophilia 13:26-31. 2007
    ..The physical therapy following operations of this sort should be regarded as the same as for acute synovitis. The rehabilitation of synovitis is independent of co-infections...
  101. pmc Inhibitory oligodeoxynucleotides downregulate herpes simplex virus-induced plasmacytoid dendritic cell type I interferon production and modulate cell function
    Wen Ming Peng
    Department of Dermatology and Allergy, University of Bonn, Bonn, Germany
    Hum Immunol 68:879-87. 2007
    ....