Research Topics
Genomes and Genes
Species | J OldenburgSummaryAffiliation: University of Bonn Country: Germany Publications
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Detail Information
Publications
Tr1 and naturally occurring regulatory T cells induce IgG4 in B cells through GITR/GITR-L interaction, IL-10 and TGF-betaJudith S Satoguina
Institute of Medical Microbiology, Immunology and Parasitology, University Clinic Bonn, Bonn, Germany
Eur J Immunol 38:3101-13. 2008..Thus, the mechanism of IgG4 induction by regulatory cells involves GITR-GITR-L interactions, IL-10 and TGF-beta...
Mutation distribution in the von Willebrand factor gene related to the different von Willebrand disease (VWD) types in a cohort of VWD patientsHamideh Yadegari
Institute of Experimental Haematology and Transfusion Medicine, University Clinics Bonn, Germany
Thromb Haemost 108:662-71. 2012..Genotyping in VWD is a helpful tool to further elucidate the pathogenesis of VWD and to establish the relationship between genotype and phenotype...
Deficiencies of antithrombin, protein C and protein S - practical experience in genetic analysis of a large patient cohortMichael Caspers
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Thromb Haemost 108:247-57. 2012..Based on our data, genetic analysis for patients with AT deficiency is indicated for all subnormal activities. In contrast, genotyping is not advisable for PC activities >70% and for PS activities >55%...
Prediction of phenprocoumon maintenance dose and phenprocoumon plasma concentration by genetic and non-genetic parametersChristof Geisen
German Red Cross, Institute of Transfusion Medicine and Immunohaematology, University Hospital Frankfurt, Frankfurt, Germany
Eur J Clin Pharmacol 67:371-81. 2011..The impact of single nucleotide polymorphisms (SNPs) in several genes of enzymes involved in the vitamin K cycle on phenprocoumon dose variability and phenprocoumon plasma concentrations is still under investigation...
Association of COMT genotypes with S-COMT promoter methylation in growth-discordant monozygotic twins and healthy adultsFelix Schreiner
Pediatric Endocrinology Division, Children s Hospital, University of Bonn, Adenauerallee 119, Bonn 53113, Germany
BMC Med Genet 12:115. 2011..So far, studies investigating the epigenetic variability of the S-COMT (soluble COMT) promoter region mainly focused on phenotypical aspects, and results have been controversial...
Novel mutations in the VKORC1 gene of wild rats and mice--a response to 50 years of selection pressure by warfarin?Simone Rost
Department of Human Genetics, University of Wuerzburg, Wuerzburg, Germany
BMC Genet 10:4. 2009..Resistance to these compounds has been reported for rodent populations from many countries around the world and poses a considerable problem for efficacy of pest control...
Haemophilia care then, now and in the futureJ Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 15:2-7. 2009..To set the scene for the following papers dealing with haemophilia care from paediatrics to geriatrics, developments behind these improvements and some aspects of future research will be presented in this paper...
[New insight in therapeutic anticoagulation by Coumarin derivatives]J Oldenburg
Institute of Experimental Haematology, and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
Hamostaseologie 28:44-50. 2008..The well established and oeconomic coumarin drugs will benefit from a pharmacogenetic and nutritive adjusted optimization of therapy...
[Congenital coagulopathies and coagulation factor inhibitors]J Oldenburg
Institut für Experimentelle Hämatologie und Transfusionsmedizin des Universitätsklinikums Bonn
Hamostaseologie 28:335-47. 2008..The Guidelines of the Federal Chamber of Physicians, the German recommendations for ITT and the consensus recommendations of an international working group provide an important lead for the conduction of immune tolerance therapy...
Genetic markers in acquired haemophiliaJ Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 16:41-5. 2010..17). This observation was mainly because of a higher frequency of the CTLA-4 + 49 G allele in female patients. These findings suggest that immune response genes may contribute to the development of anti-factor VIII autoantibodies in AH...
Thirteen novel VKORC1 mutations associated with oral anticoagulant resistance: insights into improved patient diagnosis and treatmentM Watzka
Institute of Experimental Hematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
J Thromb Haemost 9:109-18. 2011..Vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1) is the molecular target of oral anticoagulants. Mutations in VKORC1 cause partial or total coumarin resistance...
AHEAD. Advate in HaEmophilia A outcome DatabaseJ Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University Clinic, Sigmund Freud Str 25, 53127 Bonn, Germany
Hamostaseologie 30:S23-5. 2010..The first patient was enrolled in the study in early June 2010; recruitment is planned to continue until the end of 2011. The Ethics Committee of the University of Bonn has given its favorable opinion...
Discrepancy between one-stage and chromogenic factor VIII activity assay results can lead to misdiagnosis of haemophilia A phenotypeJ Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
Hamostaseologie 30:207-11. 2010..Conclusion: We propose that both methods should be used in initial patient diagnosis along with follow-up genetic analysis to avoid potential misdiagnosis and to optimize treatment monitoring of patients with HA phenotypes...
Postauthorization safety surveillance of ADVATE [antihaemophilic factor (recombinant), plasma/albumin-free method] demonstrates efficacy, safety and low-risk for immunogenicity in routine clinical practiceJ Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 16:866-77. 2010..The favourable benefit/risk profile of rAHF-PFM previously documented in prospective clinical trials has been extended to include a broader range of haemophilia patients, many of whom would have been ineligible for registration studies...
Genetic risk factors for inhibitors to factors VIII and IXJ Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 12:15-22. 2006..Improved understanding of these complex interactions may lead to the development of preventive measures to minimize inhibitor formation...
Prophylaxis in bleeding disordersJohannes Oldenburg
Institute for Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
Thromb Res 127:S14-7. 2011..Ongoing studies will help further define the role of coagulation factor concentrate prophylaxis in patients with bleeding disorders...
[Current and future prospects in haemostasis]J Oldenburg
Institut für Experimentelle Hämatologie und Transfusionsmedizin, Universitätsklinik Bonn, Sigmund Freud Str 25, 53105 Bonn
Hamostaseologie 26:13-21. 2006..Patients will profit from these developments by individualised prevention and therapy regimens, including future approaches of regenerative medicine as stem cell and gene therapy...
[Blood coagulation and hemorrhagic diathesis]Johannes Oldenburg
Pharm Unserer Zeit 35:20-8. 2006
Current pharmacogenetic developments in oral anticoagulation therapy: the influence of variant VKORC1 and CYP2C9 allelesJohannes Oldenburg
Institute for Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, D 53105 Bonn, Germany
Thromb Haemost 98:570-8. 2007..The use of new pharmacogenetics-based dosing schemes and the concomitant application of low-dose vitamin K with coumarins will decidedly influence the current practice of oral anticoagulation and greatly improve coumarin drug safety...
New insight into the molecular basis of hemophilia AJohannes Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, Bonn, Germany
Int J Hematol 83:96-102. 2006..Moreover, the proof of an absence of F8 messenger RNA (mRNA) in one patient points to either a defect in the expression of F8 mRNA or its rapid degradation, which may represent a novel mechanism leading to HA...
Molecular biology of blood coagulationJ Oldenburg
Institute for Experimental Hematology and Transfusion Services, University Clinics, Sigmund Freud Strasse 25, 53105 Bonn, Germany
Semin Thromb Hemost 27:313-24. 2001..The aim of this article is to illustrate this progress by reporting on the recent results in representative hereditary hemorrhagic and such thromboembolic conditions as hemophilia, von Willebrand disease, and thrombotic disorders...
Vitamin K epoxide reductase complex subunit 1 (VKORC1): the key protein of the vitamin K cycleJohannes Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Antioxid Redox Signal 8:347-53. 2006....
VKORC1: molecular target of coumarinsJ Oldenburg
Institute for Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
J Thromb Haemost 5:1-6. 2007..Thus, co-expression of VKORC1 leads to a more efficient production of recombinant vitamin K-dependent coagulation factors such as FIX and FVII. This could improve production of recombinant factor concentrates in the future...
[Immune tolerance therapy for inhibitors in haemophilia A]J Oldenburg
Institute of Experimental Haematology, and Transfusion Medicine University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
Hamostaseologie 28:S23-5. 2008....
HLA genotype in patients with acquired haemophilia AA Pavlova
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 16:107-12. 2010..It might be speculated that the presence or absence of the FVIII antigen and the various ability of HLA molecules to present the FVIII antigen to the T-cell receptor contribute to these findings...
Progression of liver fibrosis in HIV/HCV genotype 1 co-infected patients is related to the T allele of the rs12979860 polymorphism of the IL28B geneP Lutz
Department of Internal Medicine I, University Hospital Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany
Eur J Med Res 16:335-41. 2011..Our aim was to clarify by non-invasive techniques if this polymorphism affects fibrosis progression in HIV/HCV co-infection...
Mutations affecting disulphide bonds contribute to a fairly common prevalence of F13B gene defects: results of a genetic study in 14 families with factor XIII B deficiencyV Ivaskevicius
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 16:675-82. 2010..The present study reports on a fairly common prevalence of F13B gene defects in the German population. The regions in and around the cysteine disulphide bonds in the FXIII-B protein may be regions prone to frequent mutations...
Impact of polymorphisms of the major histocompatibility complex class II, interleukin-10, tumor necrosis factor-alpha and cytotoxic T-lymphocyte antigen-4 genes on inhibitor development in severe hemophilia AA Pavlova
Experimental Heamatology and Transfusion Medicine, University of Clinic Bonn, Sigmund Freud Str 25, Germany
J Thromb Haemost 7:2006-2015. 2009..The higher frequencies of the 308G>A polymorphism in TNF-alpha and 1082A>G in IL-10 in inhibitor patients confirmed the earlier published data. The CT60 single-nucleotide polymorphism in CTLA-4 is of apparently less importance...
[Pseudo tumours in haemophilia patients]P Berdel
Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, Sigmund Freud Strasse 25, 53127 Bonn
Hamostaseologie 29:S74-6. 2009..Conclusion: In order to reduce the complication rate when a pseudo tumor is suspected, patients should be treated in a specially equipped interdisciplinary center with adequately trained and experienced surgeons and haemostaseologists...
Identification of a third rearrangement at Xq28 that causes severe hemophilia A as a result of homologous recombination between inverted repeatsB Pezeshkpoor
Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
J Thromb Haemost 10:1600-8. 2012....
The relevance of the bleeding severity in the treatment of acquired haemophilia - an update of a single-centre experience with 67 patientsH Zeitler
Internal Medical Clinic I of the University of Bonn, Centre of Extracorporeal Therapy and Autoimmunity CETA, Bonn, Germany
Haemophilia 16:95-101. 2010..Treatment procedures should be adapted to bleeding severity and inhibitor titres. Under these conditions, AH is a potentially curable autoimmune disorder with an excellent prognosis...
Heterozygous large deletions of Factor 8 gene in females identified by multiplex PCR-LCA Pavlova
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 14:599-606. 2008..In conclusion, the multiplex PCR-LC technique represents a rapid, simple and reliable method for detection of heterozygous large deletions in female carriers...
Transient elastography discloses identical distribution of liver fibrosis in chronic hepatitis C between HIV-negative and HIV-positive patients on HAARTF Grunhage
Department of Internal Medicine I, University Hospital Bonn, Bonn, Germany
Eur J Med Res 15:139-44. 2010..Highly active antiretroviral therapy (HAART) efficiently restores and preserves immune functions and has recently been demonstrated to also result in reduced liver-related mortality in HIV/HCV-co-infected patients...
Increased frequency of the CTLA-4 49 A/G polymorphism in patients with acquired haemophilia A compared to healthy controlsA Pavlova
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 14:355-60. 2008..In conclusion, on the background of the CTLA-4 gene polymorphism, further genetic and/or environmental factors might contribute to and finally trigger the clinical manifestation of AH...
[Upper ankle joint prostheses in haemophilia patients]P Berdel
Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, Sigmund Freud Strasse 25, 53127 Bonn
Hamostaseologie 29:S65-8. 2009....
Somatic mosaicism in hemophilia A: a fairly common eventM Leuer
Department of Clinical Biochemistry, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
Am J Hum Genet 69:75-87. 2001..As a consequence, risk assessment in genetic counseling should include consideration of the possibility of somatic mosaicism in families with apparently de novo mutations, especially families with the subtype of point mutations...
Analysis of mRNA in hemophilia A patients with undetectable mutations reveals normal splicing in the factor VIII geneO El-Maarri
Institute of Experimental Haematology and Transfusion Medicine, Bonn, Germany
J Thromb Haemost 3:332-9. 2005....
Association between phenotype and genotype in carriers of haemophilia AW Miesbach
Medical Clinic III, Institute of Transfusion Medicine, Goethe University, Frankfurt, Germany
Haemophilia 17:246-51. 2011..Incidence and intensity of bleeding symptoms of haemophilia A carriers are high and correlated with the phenotype of the male haemophilic relative and the underlying F8 gene mutation...
Factor 5 mutation profile in German patients with homozygous and heterozygous factor V deficiencyD Delev
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Haemophilia 15:1143-53. 2009..Identifying the molecular basis of mutations underlying this rare coagulation disorder will allow more insight into the mechanisms involved in the variable clinical phenotypes of patients with FV deficiency...
Induction of immune tolerance in haemophilia A inhibitor patients by the 'Bonn Protocol': predictive parameter for therapy duration and outcomeJ Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Bonn, Germany
Vox Sang 77:49-54. 1999..Furthermore our data suggest, that ITT should be started at low inhibitor titres preferably with a high factor VIII dosage protocol...
Genetic predisposition to bleeding during oral anticoagulant therapy: evidence for common founder mutations (FIXVal-10 and FIXThr-10) and an independent CpG hotspot mutation (FIXThr-10)J Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Germany
Thromb Haemost 85:454-7. 2001..This strategy will provide a cost-effective and safe procedure to identify patients that carry the FIX variants. Moreover, such a strategy accumulates data about the prevalence of these FIX mutations in a given population...
Functional promoter polymorphism in the VKORC1 gene is no major genetic determinant for coronary heart disease in Northern GermansMatthias Watzka
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn Sigmund Freud Str 25, 53127 Bonn, Germany
Thromb Haemost 97:998-1002. 2007....
Maternal alleles acquiring paternal methylation patterns in biparental complete hydatidiform molesOsman El-Maarri
Institute of Experimental Haematology and Transfusion Medicine, Bonn 53105, Germany
Hum Mol Genet 12:1405-13. 2003..The defective 19q13.4 locus may have led to the development of variable degrees of 'faulty' paternal marks on the maternal chromosomes...
Inhibitor development in correlation to factor VIII genotypesJ Oldenburg
Institute of Transfusion Medicine and Immune Haematology of the DRK Blood Donor Service Hessen, Frankfurt, Germany
Haemophilia 8:23-9. 2002..However, in severely affected haemophilia A patients, the influence of patient genetics on inhibitor titre and epitope specificity has yet to be elucidated...
Frequency of contamination of coagulation factor concentrates with novel human parvovirus PARV4B Schneider
Institute of Virology, University of Bonn Medical Centre, Bonn, Germany
Haemophilia 14:978-86. 2008..Further studies are needed to determine whether or not PARV4 contamination of coagulation factors causes harm to the product recipients...
Assay discrepancy in mild haemophilia A due to a factor VIII missense mutation (Asn694Ile) in a large Danish familyR Schwaab
Institute for Experimental Haematology and Transfusion Medicine, Bonn, Germany
Br J Haematol 109:523-8. 2000..However, the current study rules out the co-existence of either von Willebrand's disease or the presence of the Normandy variant of von Willebrand factor (type 2N)...
Molecular mechanisms underlying hemophilia A phenotype in seven femalesA Pavlova
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
J Thromb Haemost 7:976-82. 2009..Hemophilia A (HA) in females is a rare observation. Here we describe various genetic mechanisms that result in phenotypic expression of HA in seven females...
Patients with familial biparental hydatidiform moles have normal methylation at imprinted genesOsman El-Maarri
Institute of Experimental Haematology and Transfusion Medicine, Bonn, Germany
Eur J Hum Genet 13:486-90. 2005....
Molecular basis of haemophilia AJ Oldenburg
Institute of Transfusion Medicine and Immunohaematology, DRK Blood Donation Service, Baden, Württemberg Hessen, D 60528 Frankfurt, Germany
Haemophilia 10:133-9. 2004..These findings raise hope for novel recombinant FVIII molecules with prolonged half-life that may improve therapies for haemophlia A...
Thrombin inhibition profiles in healthy individuals and thrombophilic patientsHeiko Rühl
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Thromb Haemost 107:848-53. 2012..In contrast, plasma levels of antithrombin falling below 80% of normal markedly prolong the thrombin half-life...
Kinetic parameters of monoclonal antibodies ESH2, ESH4, ESH5, and ESH8 on coagulation factor VIII and their influence on factor VIII activityC Egler
Center of Advanced European Studies and Research, Bonn, Germany
J Mol Recognit 22:301-6. 2009..The systematic assessment of these results should support FVIII interaction studies, and can provide data to rationally test peptides/mimotopes to remove or neutralize inhibitors of FVIII activity...
Phenotype-genotype correlation in eight Polish patients with inherited Factor XIII deficiency: identification of three novel mutationsV Ivaskevicius
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, 53127 Bonn, Germany
Haemophilia 13:649-57. 2007..In conclusion, the severe phenotype with high incidence of intracranial bleeding and haemarthrosis was in accordance with laboratory findings on FXIII and with severe molecular defects of the F13A gene...
[Muscular compartment syndrome of the forearm in a haemophilia inhibitor patient]P Berdel
Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
Hamostaseologie 28:S45-9. 2008..CONCLUSION: In order to keep the complication rate as low as possible in the presence of hemophilia with inhibitors, the patients should only be treated in a specially equipped interdisciplinary treatment center...
Correlation of transient elastography with APRI and FIB-4 in a cohort of patients with congenital bleeding disorders and HCV or HIV/HCV coinfectionN Vidovic
Institute for Experimental Haemostasiology and Transfusion Medicine, University Hospital Bonn, Bonn, Germany
Haemophilia 16:778-85. 2010..Furthermore, our data support previous studies that observed a favourable outcome in patients with HIV/HCV and a preserved immune function in times of highly active antiretroviral therapy...
Risk factors for inhibitor development in hemophilia AJ Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Germany
Haematologica 85:7-13; discussion 13-4. 2000..Fixing the genetic predisposition by gene analysis will be one important tool in the future to assess further parameters that might influence inhibitor formation...
Gene therapy of hemophiliaR Schwaab
Institute for Experimental Hematology and Transfusion Medicine, University Clinics, Sigmund Freud Str 25, 53105 Bonn, Germany
Semin Thromb Hemost 27:417-24. 2001..Encouraged by the results of preliminary experiments using preponderant mouse and canine models, three clinical phase I studies on hemophilia A and B patients have been initiated, one of which has been preliminarily reported successful...
Evaluation of DHPLC in the analysis of hemophilia AJ Oldenburg
Department of Human Genetics, University of Wurzburg, Biozentrum, Am Hubland, D 97074, Wurzburg, Germany
J Biochem Biophys Methods 47:39-51. 2001..4%), dHPLC facilitated the detection of the disease-associated nucleotide alterations. From these findings we conclude that the dHPLC technology is a highly sensitive method well suited to the molecular analysis of hemophilia A...
Lithuanian haemophilia A and B registry comprising phenotypic and genotypic dataV Ivaskevicius
Klaipeda Seamen s Hospital, Haemophilia Centre, Klaipeda, Lithuania
Br J Haematol 112:1062-70. 2001..The information provided by this registry will be helpful for monitoring the treatment of Lithuanian haemophilia patients and also for reliable genetic counselling of the affected families in the future...
[Flexion contracture in haemophilic knee arthropathy--10-year follow-up after hamstring release and dorsal capsulotomy]P H Pennekamp
Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, and St Bernhard Hospital Kamp Lintfort
Z Orthop Unfall 145:317-21. 2007..The aim of this study was to evaluate the long term results of joint preserving surgery with hamstring release and dorsal capsulotomy for the treatment of therapy resistant knee flexion contracture in patients with severe haemophilia...
[Algorithm for the treatment of the haemophilic arthropathia of the upper ankle joint]P Berdel
Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Rheinische Friedrich Wilhelms Universitat Bonn, Sigmund Freud Strase 25, 53127 Bonn
Hamostaseologie 30:S93-6. 2010..In order to minimize the complication rate, their treatment should be restricted to specially equipped interdisciplinary centers with adequately trained and experienced surgeons as well as haemostaseologists...
The first case of combined coagulation factor V and coagulation factor VIII deficiency in Poland due to a novel p.Tyr135Asn missense mutation in the MCFD2 geneVytautas Ivaskevicius
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
Blood Coagul Fibrinolysis 19:531-534. 2008..Tyr135Asn). This variant represents the third missense mutation found in the MCFD2 gene and most likely disrupts the MCFD2-LMAN1 interaction, thus leading to the disease phenotype...
Association between anxiety and factors of coagulation and fibrinolysisFranziska Geiser
Clinic for Psychosomatic Medicine and Psychotherapy, University of Bonn, Bonn, Germany
Psychother Psychosom 77:377-83. 2008..However, hemostasis function has not yet been studied in patients with clinically relevant anxiety disorders...
Effects of HCV co-infection on apoptosis of CD4+ T-cells in HIV-positive patientsChristian Korner
Department of Internal Medicine I, University of Bonn, Germany, Sigmund Freud Str 25, Bonn 53127, Germany
Clin Sci (Lond) 116:861-70. 2009..The results of the present study suggest that HCV co-infection sensitizes CD4+ T-cells towards apoptosis in untreated HIV-positive patients. However, this effect is rapidly lost under effective antiretroviral therapy...
A rapid, quantitative, non-radioactive bisulfite-SNuPE- IP RP HPLC assay for methylation analysis at specific CpG sitesOsman El-Maarri
Institute of Experimental Haematology and Transfusion Medicine, Sigmund Freud Strasse 25, 53105 Bonn, Germany
Nucleic Acids Res 30:e25. 2002..The assay is linear, highly reproducible and several sites can be measured simultaneously in one reaction. It can be semi-automated and eliminates the need for cloning and sequencing of individual bisulfite PCR products...
Subclinical hyperthyroidism seems not to have a significant impact on systemic anticoagulation in patients with coumarin therapyJan Bucerius
Department of Nuclear Medicine, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
Thromb Haemost 100:803-9. 2008..In conclusion, subclinical hyperthyroidism seems to have no significant impact on coagulation metabolism in patients receiving anticoagulation therapy...
A systematic search for DNA methyltransferase polymorphisms reveals a rare DNMT3L variant associated with subtelomeric hypomethylationOsman El-Maarri
Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
Hum Mol Genet 18:1755-68. 2009....
Lack of F8 mRNA: a novel mechanism leading to hemophilia AOsman El-Maarri
Institute of Experimental Hematology and Transfusion Medicine, Sigmund Freud Str 25, 53127 Bonn, Germany
Blood 107:2759-65. 2006..These findings strongly suggest that the cause of HA in this patient is either absence or rapid degradation of the F8 mRNA, which points to a novel mechanism leading to HA...
International registry on factor XIII deficiency: a basis formed mostly on European dataVytautas Ivaskevicius
Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, 53127 Bonn, German
Thromb Haemost 97:914-21. 2007..f13-database.de) will provide clinicians and scientists working on FXIII deficiency with a helpful tool to improve patient care and direct future studies towards better understanding and treatment of the disease...
Fifteen years of env C2V3C3 evolution in six individuals infected clonally with human immunodeficiency virus type 1Bernd Kupfer
Institute for Medical Microbiology, Immunology, and Parasitology, University of Bonn, Bonn, Germany
J Med Virol 79:1629-39. 2007..These common changes within the V3 loop are likely to be enforced by HIV-1 specific immune response...
Association of a polymorphism of the ACVRL1 gene with sporadic arteriovenous malformations of the central nervous systemMatthias Simon
Neurochirurgische Klinik and Institut für Klinische Biochemie, Universitatskliniken Bonn, Germany
J Neurosurg 104:945-9. 2006..The authors studied whether sequence variations in ACVRL1 or ENG are associated with the development of clinically sporadic arteriovenous dysplasias and aneurysms of the CNS...
Gender specific differences in levels of DNA methylation at selected loci from human total blood: a tendency toward higher methylation levels in malesOsman El-Maarri
Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Sigmund Freud Str 25, 53127, Bonn, Germany
Hum Genet 122:505-14. 2007..This observed difference could be due to the process of X chromosome inactivation or merely to the presence of an additional X chromosome in female cells or could be a result of downstream effects of sex determination...
Identification of eight novel coagulation factor XIII subunit A mutations: implied consequences for structure and functionVytautas Ivaskevicius
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, 53127 Bonn, Germany
Haematologica 95:956-62. 2010..In most cases, the disease is caused by F13A gene mutations. Causative mutations associated with the F13B gene are rarer...
Core-specific adaptive regulatory T-cells in different outcomes of hepatitis CBettina Langhans
Department of Internal Medicine I, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
Clin Sci (Lond) 119:97-109. 2010..Thus HCV core-specific Treg-cells may contribute to the immunoregulatory balance in chronic hepatitis C...
Immunoadsorption in the treatment of acquired haemophiliaHeike Zeitler
Internal Medical Clinic I of the University of Bonn, Centre of Extracorporeal Therapy and Autoimmunity, Germany
Atheroscler Suppl 10:122-5. 2009..However, the severity of bleeding, and therefore the cost-effectiveness of the approach, needs to be considered when initiating this treatment protocol...
Methylation at global LINE-1 repeats in human blood are affected by gender but not by age or natural hormone cyclesOsman El-Maarri
Institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
PLoS ONE 6:e16252. 2011..In conclusion, neither natural cycle of hormones nor age has a detectable effect on the LINE-1 methylation in peripheral blood cells, while gender remains an important factor...
Significance of F8 missense mutations with respect to inhibitor formationRainer Schwaab
Institute of Experimental Haematology and Transfusion Medicine, University Clinics Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
Thromb Haemost 109:464-70. 2013..These differences have to be identified in assigning risk profiles to aid in choice of preventative treatments designed to prevent inhibitor formation...
[Comprehensive Care Center Bonn from 1980 to 2009. Changes in the epidemiology and regional composition of the haemophilia population]I Besmens
Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Germany
Hamostaseologie 31:S4-S10. 2011..Due to the existence of well established primary haemophilia care in CCCs in Germany, the trend for the recent years is that the proportion of young patients that choose haemophilia care providers closer to their homes is increasing...
Monitoring of plasma levels of activated protein C using a clinically applicable oligonucleotide-based enzyme capture assayJ Muller
Institute for Experimental Haematology and Transfusion Medicine Clinic for Orthopedic Surgery, University Clinic Bonn Life and Medical Sciences Institute, University of Bonn, Bonn, Germany
J Thromb Haemost 10:390-8. 2012..Human-activated protein C (APC) is a serine protease with anticoagulant, anti-inflammatory and cytoprotective functions. This feature renders APC to be a promising vascular-inflammatory biomarker...
Cancer risk in HIV-infected individuals on HAART is largely attributed to oncogenic infections and state of immunocompetenceM Vogel
Department of Internal Medicine I, Bonn University, Sigmund Freud Str 25, 53105 Bonn, Germany
Eur J Med Res 16:101-7. 2011..To estimate the cancer risk of HIV-infected patients in the HAART era with respect to a general reference population and to determine risk factors for malignancy...
Quality management and quality assurance in haemophilia care: a model at the Bonn haemophilia centreH-H Brackmann
, Bonn, Germany
Haemophilia 8:211-6. 2002..All these and more are double-checked and interactive, controlling data and activities with the help of EDP. Exceptional staff motivation and patient compliance are important for this quality system...
Modified expression of coagulation factor VIII by addition of a glycosylation site at the N terminus of the proteinM A Srour
Institute for Experimental Hematology and Transfusion Medicine, University Clinics Bonn, University of Bonn, Sigmund Freud Str 25, 53105, Bonn, Germany
Ann Hematol 87:107-12. 2008..Thus, glycosylation alone does not automatically lead to higher secretion rates, but must be in context to the normal structure of the FVIII protein...
Modelling and expression studies of two novel mutations causing factor V deficiencyDaniel Delev
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
Thromb Haemost 100:766-72. 2008..Thus, both the data from the short duration molecular dynamic simulation and from expression analysis indicate alterations of the FV protein variants that explain the clinical phenotype...
The vitamin K cycleJohannes Oldenburg
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, D 53105 Bonn, Germany
Vitam Horm 78:35-62. 2008....
Effects of the CCR5-Delta32 mutation on hepatitis C virus-specific immune responses in patients with haemophiliaGolo Ahlenstiel
Department of Internal Medicine I, University of Bonn, Bonn, Germany
Immunol Invest 38:284-96. 2009..Interruption of the CCR5 signalling pathway due to CCR5-Delta32 may potentially result in subtle reduction of HCV specific IFN-gamma responses in anti-HCV-positive haemophiliac patients...
Perioperative management and outcome of general and abdominal surgery in hemophiliacsGeorg Goldmann
Institute for Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
Am J Surg 199:702-7. 2010..The aim of the current study was to investigate perioperative management and outcome of surgery in hemophiliacs...
Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatographyJens Muller
Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Sigmund Freud Str 25, 53127, Bonn, Germany
Anal Bioanal Chem 390:1033-7. 2008..We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences...
The coagulation system of extremely preterm infants: influence of perinatal risk factors on coagulationC Poralla
Department of Neonatology, University of Bonn, Bonn, Germany
J Perinatol 32:869-73. 2012..The objective of this study was to determine reference values for the components of the coagulation system at the first day of life in extremely preterm infants...
Rehabilitation of synovitis in patients with haemophiliaA Seuser
Orthopaedic Department, Kaiser Karl Klinik, Special Hospital for Rehabilitation Medicine, Bonn, Germany
Haemophilia 13:26-31. 2007..The physical therapy following operations of this sort should be regarded as the same as for acute synovitis. The rehabilitation of synovitis is independent of co-infections...
4beta-hydroxycholesterol as a marker of CYP3A4 inhibition in vivo - effects of itraconazole in manD Lutjohann
Institute of Clinical Chemistry and Pharmacology, University Clinic, Bonn, Germany
Int J Clin Pharmacol Ther 47:709-15. 2009..Here, we study the potential endogenous serum marker of CYP3A4 activity, 4beta-hydroxycholesterol, during therapy with itraconazole...
Inhibitory oligodeoxynucleotides downregulate herpes simplex virus-induced plasmacytoid dendritic cell type I interferon production and modulate cell functionWen Ming Peng
Department of Dermatology and Allergy, University of Bonn, Bonn, Germany
Hum Immunol 68:879-87. 2007....
Relative hyperhomocysteinemia in patients with panic disorder: a case-control studyChristian Meier
Clinic for Psychosomatic Medicine and Psychotherapy, University of Bonn, Bonn, Germany
Neuropsychobiology 62:164-70. 2010..The aim of this work was to examine a possible association between a clinically relevant panic disorder and plasma total homocysteine concentration...
VKORC1 deficiency in mice causes early postnatal lethality due to severe bleedingGabriele Spohn
Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
Thromb Haemost 101:1044-50. 2009..Thus, our study underscores the essential role of VKORC1 in vitamin K-dependent gamma-glutamyl carboxylation...
An exosite-specific ssDNA aptamer inhibits the anticoagulant functions of activated protein C and enhances inhibition by protein C inhibitorJens Muller
Institute for Experimental Haematology and Transfusion Medicine, University Hospital Bonn, D 53105 Bonn, Germany
Chem Biol 16:442-51. 2009..These characteristics render the exosite-specific aptamers a promising tool for the development of APC assays and a potential therapeutic agent applicable for the selective control of APC's anticoagulant activity...
Characterization of duplication breakpoints in the factor VIII geneM A Zimmermann
Department of Human Genetics, University of Wuerzburg, Wuerzburg, Germany
J Thromb Haemost 8:2696-704. 2010..Large duplications comprising whole exons of F8 have been published for only a few cases so far...
Regulation of human factor IX expression using doxycycline-inducible gene expression systemMahmoud A Srour
Institute for Experimental Hematology and Transfusion Medicine, University Clinics Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
Thromb Haemost 90:398-405. 2003..Although hFIX expression showed a slow and gradual decrease over time in vivo with the Ad5.CMV.rtTA vector, it remained within the therapeutic range. To date, regulation of hFIX has not been described in this way...
Human plasmacytoid dendritic cells support Th17 cell effector function in response to TLR7 ligationChun Feng Yu
Department of Dermatology and Allergy, University Hospital, University of Bonn, Bonn, Germany
J Immunol 184:1159-67. 2010....
Identification of 32 novel mutations in the factor VIII gene in Indian patients with hemophilia ARafeeq P H Ahmed
Haematologica 90:283-4. 2005..These included 14 missense, 7 nonsense, 9 splice site, 8 deletional, 3 insertional mutations and one indel mutation. Of these, 32 were novel gene alterations. The hotspots included intron 22 inversion, CpG and adenine runs...
Polymorphisms in the TNFA gene and the risk of inhibitor development in patients with hemophilia AJan Astermark
Department for Coagulation Disorders, Malmo University Hospital, SE 205 02 Malmo, Sweden
Blood 108:3739-45. 2006..Our data imply that the TNFA -308G>A polymorphism within Hap 2 is a useful marker and potential modulator of the immune response to replacement therapy in patients with hemophilia...
The genetic basis of resistance to anticoagulants in rodentsHans Joachim Pelz
Biologische Bundesanstalt für Land und Forstwirtschaft, Institut für Nematologie und Wirbeltierkunde, 48161 Münster, Germany
Genetics 170:1839-47. 2005..Our findings provide the basis for a DNA-based field monitoring of anticoagulant resistance in rodents...
Vitamin K intake and stability of oral anticoagulant treatmentJohannes Oldenburg
Thromb Haemost 93:799-800. 2005
Haemophilia A: from mutation analysis to new therapiesJochen Graw
GSF National Research Centre for Environment and Health, Institute of Developmental Genetics, D 85764 Neuherberg, Germany
Nat Rev Genet 6:488-501. 2005..Haemophilia is also the most attractive model for developing gene-therapy protocols, as the normal life expectancy of haemophiliacs allows the side effects of gene therapy, as well as its efficiency, to be monitored over long periods...
