Christof M Niemeyer

Summary

Affiliation: University of Dortmund
Country: Germany

Publications

  1. ncbi request reprint A single-molecule Förster resonance energy transfer analysis of fluorescent DNA-protein conjugates for nanobiotechnology
    Florian Kukolka
    Fachbereich Chemie Biologisch Chemische Mikrostrukturtechnik, Universitat Dortmund, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Small 2:1083-9. 2006
  2. doi request reprint Synthesis of covalent oligonucleotide-streptavidin conjugates and their application in DNA-directed immobilization (DDI) of proteins
    Ron Wacker
    Chimera Biotec GmbH, Dortmund, Germany
    Curr Protoc Nucleic Acid Chem . 2005
  3. ncbi request reprint Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplification
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Lehrstuhl für Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Trends Biotechnol 23:208-16. 2005
  4. ncbi request reprint Functional hybrid devices of proteins and inorganic nanoparticles
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 42:5796-800. 2003
  5. pmc Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA-protein conjugates
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, D 44227 Dortmund, Germany
    Nucleic Acids Res 31:e90. 2003
  6. ncbi request reprint Semi-synthetic DNA-protein conjugates: novel tools in analytics and nanobiotechnology
    C M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Biochem Soc Trans 32:51-3. 2004
  7. ncbi request reprint Oligofunctional DNA-gold nanoparticle conjugates
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 42:5766-70. 2003
  8. ncbi request reprint Nanomechanical devices based on DNA
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 41:3779-83. 2002
  9. ncbi request reprint Supramolecular DNA-streptavidin nanocircles with a covalently attached oligonucleotide moiety
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund
    J Biomol Struct Dyn 20:223-30. 2002
  10. ncbi request reprint The developments of semisynthetic DNA-protein conjugates
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Trends Biotechnol 20:395-401. 2002

Collaborators

Detail Information

Publications83

  1. ncbi request reprint A single-molecule Förster resonance energy transfer analysis of fluorescent DNA-protein conjugates for nanobiotechnology
    Florian Kukolka
    Fachbereich Chemie Biologisch Chemische Mikrostrukturtechnik, Universitat Dortmund, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Small 2:1083-9. 2006
    ..Hence, DNA can be used as a scaffold for positioning fluorescent proteins, as well as traditional fluorophores, with nanometer accuracy and shows great potential for use in the future of nanobiotechnology...
  2. doi request reprint Synthesis of covalent oligonucleotide-streptavidin conjugates and their application in DNA-directed immobilization (DDI) of proteins
    Ron Wacker
    Chimera Biotec GmbH, Dortmund, Germany
    Curr Protoc Nucleic Acid Chem . 2005
    ..The results are protein microarrays that can be used for the multiplexed detection of various antigens...
  3. ncbi request reprint Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplification
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Lehrstuhl für Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Trends Biotechnol 23:208-16. 2005
    ..Eventually, IPCR crossed the border from being a research method to a routine laboratory technique, enabling a broad range of applications in immunological research and clinical diagnostics...
  4. ncbi request reprint Functional hybrid devices of proteins and inorganic nanoparticles
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 42:5796-800. 2003
  5. pmc Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA-protein conjugates
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, D 44227 Dortmund, Germany
    Nucleic Acids Res 31:e90. 2003
    ..Moreover, as the spatial selectivity of target immobilization is determined by the specificity of DNA base pairing, the assay is particularly suited for miniaturized microfluidics and lab-on-a-chip devices...
  6. ncbi request reprint Semi-synthetic DNA-protein conjugates: novel tools in analytics and nanobiotechnology
    C M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Biochem Soc Trans 32:51-3. 2004
    ..Examples include the fabrication of functional biometallic nanostructures from gold nanoparticles and antibodies, applicable as diagnostic tools in bioanalytics...
  7. ncbi request reprint Oligofunctional DNA-gold nanoparticle conjugates
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 42:5766-70. 2003
  8. ncbi request reprint Nanomechanical devices based on DNA
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 41:3779-83. 2002
  9. ncbi request reprint Supramolecular DNA-streptavidin nanocircles with a covalently attached oligonucleotide moiety
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund
    J Biomol Struct Dyn 20:223-30. 2002
    ..Moreover, we demonstrate that a pair of nanocircles, containing complementary oligonucleotide moieties, can be hybridized to form specific dimers, thereby generating a novel type of supramolecular DNA-protein nanostructures...
  10. ncbi request reprint The developments of semisynthetic DNA-protein conjugates
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Trends Biotechnol 20:395-401. 2002
    ....
  11. ncbi request reprint Nanotechnology. Tools for the biomolecular engineer
    Christof M Niemeyer
    Chemistry Department, University of Dortmund, 44227 Dortmund, Germany
    Science 297:62-3. 2002
  12. doi request reprint Preparation of biomolecule microstructures and microarrays by thiol-ene photoimmobilization
    Dirk Weinrich
    Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Chembiochem 11:235-47. 2010
    ..Use of a confocal laser microscope with a UV laser as UV-light source enabled further reduction of biotin feature size opening access to nanostructured biochips...
  13. doi request reprint Engineering and assaying of cytochrome P450 biocatalysts
    Kersten S Rabe
    Fakultat fur Chemie, Biologisch Chemische Mikrostrukturtechnik, Technische Universitat Dortmund, Otto Hahn Strabetae 6, 44227, Dortmund, Germany
    Anal Bioanal Chem 392:1059-73. 2008
    ..To this end, recent advances in cytochrome P450 development are reported and commonly used screening methods are surveyed...
  14. ncbi request reprint Semisynthetic biogenic magnetosome nanoparticles for the detection of proteins and nucleic acids
    Buelent Ceyhan
    Universitat Dortmund, Fachbereich Chemie Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Small 2:1251-5. 2006
  15. doi request reprint Photocatalytic activity of protein-conjugated CdS nanoparticles
    Vidyalakshmi Rajendran
    Biologisch Chemische Mikrostrukturtechnik, Technische Universitat Dortmund, Dortmund, Germany
    Small 6:2035-40. 2010
    ..The empirical results are capitalized on for the assembly of a CdS-TEV nanohybrid, which shows significantly higher performance as a photocatalytic mediator for fatty acid hydroxylation by CYP152A1 than bare CdS nanoparticles...
  16. ncbi request reprint Screening for cytochrome p450 reactivity by harnessing catalase as reporter enzyme
    Kersten S Rabe
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, Dortmund, Germany
    Chembiochem 10:751-7. 2009
    ....
  17. ncbi request reprint On the generation of free radical species from quantum dots
    Binil Itty Ipe
    Biologisch Chemische Mikrostrukturtechnik, Fachbereich Chemie, Universitat Dortmund, Otto Hahn Str 6, 44227, Dortmund, Germany
    Small 1:706-9. 2005
  18. ncbi request reprint DNA-directed immobilization of horseradish peroxidase-DNA conjugates on microelectrode arrays: towards electrochemical screening of enzyme libraries
    Ljiljana Fruk
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Chemistry 13:5223-31. 2007
    ..Therefore, the self-assembly and regeneration capabilities of this approach should open the door to the generation of arrays of redox-enzyme devices for the screening of enzymes and their effectors...
  19. ncbi request reprint Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: a comparative study
    Ron Wacker
    Chimera Biotec GmbH, Emil Figge Strasse 76A, D 44227 Dortmund, Germany
    Anal Biochem 330:281-7. 2004
    ..Taking into account the greater versatility and convenience of handling of the self-assembly approach, this study demonstrates that DDI is an advantageous alternative for generating versatile and robust protein arrays...
  20. doi request reprint Conjugation of fluorescent proteins with DNA oligonucleotides
    Vidmantas Lapiene
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Bioconjug Chem 21:921-7. 2010
    ..Since this study demonstrates that biological fluorophores can be selectively incorporated into and optically coupled with nanoparticle-based devices, applications in DNA-based nanofabrication can be foreseen...
  21. ncbi request reprint Sensitive detection of proteins using difunctional DNA-gold nanoparticles
    Pompi Hazarika
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, 44227 Dortmund, Germany
    Small 1:844-8. 2005
  22. ncbi request reprint A real-time immuno-PCR assay for routine ultrasensitive quantification of proteins
    Michael Adler
    Chimera Biotec GmbH, Emil Figge Str 76 A, D 44227 Dortmund, Germany
    Biochem Biophys Res Commun 308:240-50. 2003
    ..In both cases, a comparable ELISA was 1000-fold less sensitive...
  23. ncbi request reprint A microarray strategy for mapping the substrate specificity of protein tyrosine phosphatase
    Maja Köhn
    Department of Chemical Biology, Max Planck Institute für Molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 46:7700-3. 2007
  24. ncbi request reprint Direct readout of protein-protein interactions by mass spectrometry from protein-DNA microarrays
    Christian F W Becker
    Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 44:7635-9. 2005
  25. ncbi request reprint Selective covalent conjugation of phosphorothioate DNA oligonucleotides with streptavidin
    Kersten S Rabe
    TU Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Molecules 16:6916-26. 2011
    ..The psDNA-STV therefore hold particular promise for applications e.g. in proteome research and novel biosensing devices, where interfering endogenous nucleic acids need to be removed from analytes by nuclease digestion...
  26. ncbi request reprint A generic building block for C- and N-terminal protein-labeling and protein-immobilization
    Anja Watzke
    Max Planck Institut fur molekulare Physiologie, Abteilung Chemische Biologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Bioorg Med Chem 14:6288-306. 2006
    ..Azide-functionalized Ras protein was immobilized on a phosphane-modified surface by means of Staudinger ligation providing a highly chemoselective ligation method for the immobilization of proteins...
  27. ncbi request reprint Functional immobilization of the small GTPase Rab6A on DNA-Gold nanoparticles by using a site-specifically attached poly(ethylene glycol) linker and thiol place-exchange reaction
    Christian F W Becker
    Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Chembiochem 8:32-6. 2007
  28. doi request reprint Self-assembled donor comprising quantum dots and fluorescent proteins for long-range fluorescence resonance energy transfer
    Huachang Lu
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    J Am Chem Soc 130:4815-27. 2008
    ....
  29. ncbi request reprint Microtiter plate-based screening for the optimization of DNA-protein conjugate synthesis by means of expressed protein ligation
    Marina Lovrinovic
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Chembiochem 8:61-7. 2007
    ..As the consequence of the assay-based optimization, the ligation of MBP with the oligonucleotide was improved to near quantitative yields...
  30. doi request reprint Photochemical surface patterning by the thiol-ene reaction
    Pascal Jonkheijm
    Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 47:4421-4. 2008
  31. doi request reprint Tuning of peroxidase activity by covalently tethered DNA oligonucleotides
    Maximilian Glettenberg
    Technische Universitat Dortmund, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, D 44227 Dortmund, Germany
    Bioconjug Chem 20:969-75. 2009
    ....
  32. doi request reprint DNA-directed assembly of artificial multienzyme complexes
    Joachim Muller
    Fakultat Chemie, Technische Universitat Dortmund, Biologisch Chemische Mikrostrukturtechnik, Dortmund, Germany
    Biochem Biophys Res Commun 377:62-7. 2008
    ..The kinetic measurements revealed a significant increase in the reactivity of the complexes, in which GOX and HRP were immobilized in direct proximity on a complementary DNA carrier...
  33. doi request reprint Apoenzyme reconstitution as a chemical tool for structural enzymology and biotechnology
    Ljiljana Fruk
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 48:1550-74. 2009
    ..This Review gives an overview on historical developments and the current state-of-the-art on apoenzyme reconstitution...
  34. doi request reprint Immuno-PCR assays for immunogenicity testing
    Mark Spengler
    Chimera Biotec GmbH, D 44227 Dortmund, Germany
    Biochem Biophys Res Commun 387:278-82. 2009
    ..These results suggest that IPCR has the potential to become a standard methodology in immunogenicity testing...
  35. ncbi request reprint Magneto immuno-PCR: a novel immunoassay based on biogenic magnetosome nanoparticles
    Ron Wacker
    Chimera Biotec GmbH, Emil Figge Str 76A, D 44227 Dortmund, Germany
    Biochem Biophys Res Commun 357:391-6. 2007
    ..We observed a detection limit of 320pg/ml of HBsAg using the M-IPCR, which was about 100-fold more sensitive than the analogous Magneto-ELISA, established in parallel for comparison purposes...
  36. ncbi request reprint Synthesis and characterization of deoxyribonucleic acid-conjugated gold nanoparticles
    Pompi Hazarika
    Biologisch Chemische Mikrostrukturtechnik, Fachbereich Chemie der Universität Dortmund, Germany
    Methods Mol Biol 283:295-304. 2004
    ..The hybridization capabilities are quantified in a microplate assay...
  37. ncbi request reprint Reversible binding of fluorescent proteins at DNA-gold nanoparticles
    Pompi Hazarika
    Biologisch Chemische Mikrostrukturtechnik, Fachbereich Chemie, Universitat Dortmund, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 45:6827-30. 2006
  38. doi request reprint Temperature-dependent FRET spectroscopy for the high-throughput analysis of self-assembled DNA nanostructures in real time
    Barbara Saccà
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, D 44227 Dortmund, Germany
    Nat Protoc 4:271-85. 2009
    ..Owing to slow temperature ramping, the entire assay requires about 17 h. The protocol allows to simultaneously analyze up to 384 samples with only 30 microl sample volume each...
  39. ncbi request reprint Generation of live-cell microarrays by means of DNA-Directed immobilization of specific cell-surface ligands
    Hendrik Schroeder
    Biologisch Chemische Mikrostrukturtechnik, Fachbereich Chemie, Universitat Dortmund, Dortmund, Germany
    Angew Chem Int Ed Engl 46:4180-3. 2007
  40. doi request reprint Rational engineering of dynamic DNA systems
    Udo Feldkamp
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 47:3871-3. 2008
  41. ncbi request reprint Addressable DNA-myoglobin photocatalysis
    Chi Hsien Kuo
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, 44227 Dortmund, Germany
    Chem Asian J 4:1064-9. 2009
    ..Enzyme activity measurements not only indicated undisturbed biofunctionality of the tethered DNA but also enabled magnetic separation-based enrichment and recycling of the photoactivatable biocatalyst...
  42. doi request reprint Peroxidase activity of bacterial cytochrome P450 enzymes: modulation by fatty acids and organic solvents
    Kersten S Rabe
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Dortmund, Germany
    Biotechnol J 5:891-9. 2010
    ..In general, our detailed description of fatty acid- and organic solvent-effects is of practical interest because it illustrates that optimization of modulators and cosolvents can lead to significantly increased yields in biocatalysis...
  43. ncbi request reprint Analysis of non-covalent bioconjugation of colloidal nanoparticles by means of atomic force microscopy and data clustering
    Jessica Irrgang
    Biologisch Chemische Mikrostrukturtechnik, Fakultat Chemie, Technische Universitat Dortmund, Otto Hahn Str 6, 44227 Dortmund, Germany
    Chemphyschem 10:1483-91. 2009
    ..Therefore, this analytical approach is useful to elucidate bioconjugate species, formed in the course of non-covalent synthesis of nanoparticle-biomolecule hybrid systems...
  44. ncbi request reprint Addressable microfluidic polymer chip for DNA-directed immobilization of oligonucleotide-tagged compounds
    Hendrik Schröder
    Technische Universitat Dortmund, Fakultät Chemie Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, 44227 Dortmund, Germany
    Small 5:1547-52. 2009
    ..The results demonstrate that this addressable chip is well suited for the generation of fluidically coupled multi-enzyme microreactors...
  45. ncbi request reprint Adaptation and performance of an immuno-PCR assay for the quantification of Aviscumine in patient plasma samples
    Michael Adler
    Chimera Biotec GmbH, Emil Figge Str 76 A, D 44227 Dortmund, Germany
    J Pharm Biomed Anal 39:972-82. 2005
    ..The collected data provided the basis for the valid routine quantification of patient samples for the calculation of the pharmacokinetic behaviour of Aviscumine in patient plasma...
  46. doi request reprint Analysis of the self-assembly of 4x4 DNA tiles by temperature-dependent FRET spectroscopy
    Barbara Saccà
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, 44227 Dortmund, Germany
    Chemphyschem 10:3239-48. 2009
    ..Therefore, the microplate FRET method readily provides insights into the assembly process of complex DNA superstructures to verify and complement theoretical design approaches...
  47. ncbi request reprint Chemical strategies for generating protein biochips
    Pascal Jonkheijm
    Department of Chemical Biology, Max Planck Institute of Molecular Physiology and Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 47:9618-47. 2008
    ..Strategies for creating protein patterns (as opposed to protein arrays) are also outlined. An outlook on promising and challenging future directions for protein biochip research and applications is also offered...
  48. doi request reprint Nanolattices of switchable DNA-based motors
    Barbara Saccà
    TU Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Small 8:3000-8. 2012
    ..Altogether, our study confirms that DNA superstructures are well-suited scaffolds for accommodation of mechanically switchable units and thus opens the door to the development of more sophisticated nanomechanical devices...
  49. doi request reprint Direct immobilization of oxyamine-modified proteins from cell lysates
    Long Yi
    Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, D 44227 Dortmund, Germany
    Chem Commun (Camb) 48:10829-31. 2012
    ..Oxyamine-modified proteins can be efficiently and selectively immobilized on ketone-coated glass slides at neutral pH in short reaction times by direct treatment and spotting of protein expression lysates without prior purification...
  50. doi request reprint Orthogonal protein decoration of DNA nanostructures
    Rebecca Meyer
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund
    Small 7:3211-8. 2011
    ....
  51. doi request reprint DNA-mediated assembly of cytochrome P450 BM3 subdomains
    Michael Erkelenz
    TU Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, D 44227 Dortmund, Germany
    J Am Chem Soc 133:16111-8. 2011
    ....
  52. doi request reprint Applications of protein biochips in biomedical and biotechnological research
    Dirk Weinrich
    Max Planck Institut für molekulare Physiologie and Technische Universität Dortmund, Fachbereich Chemie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 48:7744-51. 2009
    ..This Minireview highlights selected developments in the application of protein biochips in these fields...
  53. ncbi request reprint Bifunctional DNA-gold nanoparticle conjugates as building blocks for the self-assembly of cross-linked particle layers
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227, Dortmund, Germany
    Biochem Biophys Res Commun 311:995-9. 2003
    ..We anticipate that further refinement of this approach will enable applications, for instance, the assembly of ordered layers useful as transducers in biosensing...
  54. doi request reprint User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly
    Hendrik Schroeder
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, 44227 Dortmund, Germany
    Anal Chem 81:1275-9. 2009
    ..We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and optical read-out...
  55. doi request reprint High-throughput, real-time monitoring of the self-assembly of DNA nanostructures by FRET spectroscopy
    Barbara Saccà
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 47:2135-7. 2008
  56. ncbi request reprint DDI-microFIA--A readily configurable microarray-fluorescence immunoassay based on DNA-directed immobilization of proteins
    Ron Wacker
    Chimera Biotec GmbH, Emil Figge Strasse 76 A, 44227 Dortmund, Germany
    Chembiochem 5:453-9. 2004
    ..This protocol significantly reduces handling time and costs of analysis...
  57. ncbi request reprint Synthesis of fluorescent oligonucleotide--EYFP conjugate: towards supramolecular construction of semisynthetic biomolecular antennae
    Florian Kukolka
    Universitat Dortmund, Fachbereich Chemie Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Org Biomol Chem 2:2203-6. 2004
    ..These studies are paving the way for future applications in the self-assembly of photoactive supramolecular complexes, such as artificial light-harvesting systems...
  58. ncbi request reprint Design and evaluation of single-stranded DNA carrier molecules for DNA-directed assembly
    Udo Feldkamp
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    J Biomol Struct Dyn 23:657-66. 2006
    ..Hybridization efficiency of DNA-STV-conjugates depends on the position of the hybridization site in the carrier sequence, where the positions nearest to and farthest from the microarray surface proved to be most favorable...
  59. ncbi request reprint Synthesis of covalent DNA-protein conjugates by expressed protein ligation
    Marina Lovrinovic
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund
    Mol Biosyst 1:64-9. 2005
    ..We anticipate that our concept will enable many applications, such as the generation of large arrays of surface-bound, recombinant proteins assembled by means of DNA-directed immobilization...
  60. ncbi request reprint Rational design of DNA nanoarchitectures
    Udo Feldkamp
    Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Universitat Dortmund, Germany
    Angew Chem Int Ed Engl 45:1856-76. 2006
    ..This Review is intended to provide an overview of this fascinating and rapidly growing field of research from the structural design point of view...
  61. ncbi request reprint DNA-directed assembly of supramolecular fluorescent protein energy transfer systems
    Florian Kukolka
    Fachbereich Chemie Biologisch Chemische Mikrostrukturtechnik, Universitat Dortmund, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Bioconjug Chem 18:621-7. 2007
    ....
  62. ncbi request reprint Detecting antigens by quantitative immuno-PCR
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Lehrstuhl für Biologisch Chemische Mikrostrukturtechnik, Dortmund, Germany
    Nat Protoc 2:1918-30. 2007
    ..Therefore, qIPCR offers novel opportunities for the biomedical analysis of, for instance, neurodegenerative diseases and viral infections as well as new tools for the development of novel pharmaceuticals...
  63. doi request reprint Functionalization of DNA nanostructures with proteins
    Barbara Saccà
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Dortmund, Germany
    Chem Soc Rev 40:5910-21. 2011
    ..This critical review provides an overview on chemical approaches to the synthesis of DNA-protein conjugates and their applications in biomolecular nanosciences (96 references)...
  64. doi request reprint Semisynthetic DNA-protein conjugates for biosensing and nanofabrication
    Christof M Niemeyer
    Technische Universitat Dortmund, Fakultat Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 49:1200-16. 2010
    ..Such hybrid conjugates are also used for the biofunctionalization of planar surfaces for micro- and nanoarrays, and for decorating inorganic nanoparticles to enable applications in sensing, materials science, and catalysis...
  65. ncbi request reprint Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
    Ljiljana Fruk
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Chemistry 12:7448-57. 2006
    ....
  66. ncbi request reprint Dynamic light-scattering analysis of the electrostatic interaction of hexahistidine-tagged cytochrome P450 enzyme with semiconductor quantum dots
    Binil Itty Ipe
    Biologisch Chemische Mikrostrukturtechnik, Fachbereich Chemie, Universitat Dortmund, Otto Hahn Str 6, 44227 Dortmund, Germany
    Chemphyschem 7:1112-8. 2006
    ....
  67. ncbi request reprint Microarray-based in vitro evaluation of DNA oligomer libraries designed in silico
    Udo Feldkamp
    Universitat Dortmund, Fachbereich Informatik, LS 11 44221 Dortmund, Germany
    Chemphyschem 5:367-72. 2004
    ....
  68. ncbi request reprint Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius
    Kersten S Rabe
    Technische Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Dortmund, Germany
    Chembiochem 9:420-5. 2008
    ..The values obtained for k(cat) (78.2+/-20.6 min(-1)) and K(M) (9.2+/-4.3 mM) indicated an approximately 100-fold increased catalytic activity over previously reported results...
  69. ncbi request reprint Covalent coupling of DNA oligonucleotides and streptavidin
    Florian Kukolka
    Biologisch Chemische Mikrostrukturtechnik, Fachbereich Chemie der Universität Dortmund, Germany
    Methods Mol Biol 283:181-96. 2004
    ..Subsequently, the conjugates are applied as molecular linkers in the DNA-directed immobilization of a biotinylated enzyme on a microplate, containing complementary capture oligonucleotides...
  70. ncbi request reprint Staudinger ligation: a new immobilization strategy for the preparation of small-molecule arrays
    Maja Köhn
    Department of Chemical Biology, Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 42:5830-4. 2003
  71. ncbi request reprint Reversible switching of DNA-gold nanoparticle aggregation
    Pompi Hazarika
    Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Universitat Dortmund, Dortmund, Germany
    Angew Chem Int Ed Engl 43:6469-71. 2004
  72. ncbi request reprint Detection of rViscumin in plasma samples by immuno-PCR
    Michael Adler
    Chimera Biotec GmbH, Emil Figge Str 76 A, D 44227 Dortmund, Germany
    Biochem Biophys Res Commun 300:757-63. 2003
    ..The linear sensitivity range of the assay covered more than five orders of magnitude. Repeated measurements of rViscumin resulted in a mean standard deviation value of 14.2%...
  73. ncbi request reprint Evaluation of cytochrome P450(BSbeta) reactivity against polycyclic aromatic hydrocarbons and drugs
    Eduardo Torres
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Biochem Biophys Res Commun 355:286-93. 2007
    ..Overall, the results of this study suggested that the P450(BSbeta) enzyme represents a powerful catalyst in terms of the catalytic activity and operational stability...
  74. ncbi request reprint Rapid synthesis of DNA-cysteine conjugates for expressed protein ligation
    Marina Lovrinovic
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund, Germany
    Biochem Biophys Res Commun 335:943-8. 2005
    ..We anticipate many applications of our approach, ranging from protein microarrays to the arising field of nanobiotechnology...
  75. pmc DNA microarrays with PAMAM dendritic linker systems
    Rüdiger Benters
    Chimera Biotec GmbH, Schwachhauser Heerstrasse 30A, 28209 Bremen, Germany
    Nucleic Acids Res 30:E10. 2002
    ..The performance of dendrimer-based DNA microarrays in the discrimination of SNPs is demonstrated...
  76. ncbi request reprint DNA-directed assembly of bienzymic complexes from in vivo biotinylated NAD(P)H:FMN oxidoreductase and luciferase
    Christof M Niemeyer
    Universitat Bremen, FB2 UFT Biotechnologie und Molekulare Genetik, Leobener Strasse, 28359 Bremen, Germany
    Chembiochem 3:242-5. 2002
  77. doi request reprint Sensitivity by combination: immuno-PCR and related technologies
    Michael Adler
    Chimera Biotec GmbH, Emil Figge Str 76 A, D 44227, Dortmund, Germany
    Analyst 133:702-18. 2008
    ..The examples include the detection of viral and bacterial antigens, tumor markers, toxins, pathogens, cytokines and other targets in different biological sample materials...
  78. ncbi request reprint Monte Carlo simulation of the assembly of bis-biotinylated DNA and streptavidin
    Jan Richter
    Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver V6T 1Z1, Canada
    Chemphyschem 4:79-83. 2003
    ..This behaviour coincides with the occurrence of an immobile band previously observed in electrophoretic experiments, indicating the formation of extremely large DNA-STV aggregate networks...
  79. ncbi request reprint Site-selective protein immobilization by Staudinger ligation
    Anja Watzke
    Department of Chemical Biology, Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44 227 Dortmund, Germany
    Angew Chem Int Ed Engl 45:1408-12. 2006
  80. ncbi request reprint Nanohybrids composed of quantum dots and cytochrome P450 as photocatalysts
    Binil Itty Ipe
    Biologisch Chemische Mikrostrukturtechnik, Fachbereich Chemie, Universitat Dortmund, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 45:504-7. 2006
  81. ncbi request reprint DNA microarrays as decoding tools in combinatorial chemistry and chemical biology
    Marina Lovrinovic
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 44:3179-83. 2005
  82. ncbi request reprint Covalent hemin-DNA adducts for generating a novel class of artificial heme enzymes
    Ljiljana Fruk
    Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Universitat Dortmund, Otto Hahn Strasse 6, 44227 Dortmund, Germany
    Angew Chem Int Ed Engl 44:2603-6. 2005
  83. ncbi request reprint Detection of Rotavirus from stool samples using a standardized immuno-PCR ("Imperacer") method with end-point and real-time detection
    Michael Adler
    Chimera Biotec GmbH, Emil Figge Str 76 A, D 44227 Dortmund, Germany
    Biochem Biophys Res Commun 333:1289-94. 2005
    ..Rotavirus positive and negative stool samples were correctly identified by IPCR with a clear separation even of a 10,000-fold dilution of the positive stool samples from the negative control...