Gunter Mayer

Summary

Affiliation: University of Bonn
Country: Germany

Publications

  1. ncbi Light regulation of aptamer activity: an anti-thrombin aptamer with caged thymidine nucleobases
    Alexander Heckel
    Kekulé Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard Domagk Str 1, 53121 Bonn, Germany
    J Am Chem Soc 127:822-3. 2005
  2. ncbi [Nucleic acids in the drug discovery process: aptamers as therapeutic and as active ingredients for drugs]
    Gunter Mayer
    Rheinische Friedrich Wilhelms Universitat Bonn, LIMES Institut, Programmeinheit Chemische Biologie und Medizinische Chemie, c o Kekulé Institut für Org Chemie und Biochemie, Gerhard Domagk Str 1, 53121 Bonn
    Pharm Unserer Zeit 36:432-6. 2007
  3. ncbi Differential regulation of protein subdomain activity with caged bivalent ligands
    Gunter Mayer
    Life and Medical Sciences, Program Unit Chemical Biology and Medicinal Chemistry, University of Bonn c o Kekulé Institute for Organic Chemistry and Biochemistry, Gerhard Domagk Strasse 1, Bonn, Germany
    Chembiochem 10:654-7. 2009
  4. ncbi Light-stimulated patterning of cells
    Gunter Mayer
    University of Bonn, LIMES Life and Medical Sciences, c o Kekulé Institute, Gerhard Domagk Strasse 1, Bonn, Germany
    Chembiochem 8:857-8. 2007
  5. doi The chemical biology of aptamers
    Gunter Mayer
    Life and Medical Sciences, Prog Unit Chemical Biology and Medicinal Chemistry, University of Bonn c o Kekulé Institute for Organic Chemistry and Biochemistry, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 48:2672-89. 2009
  6. pmc An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro
    Gunter Mayer
    Life and Medical Sciences Bonn, Program Unit Chemical Biology, c o Kekulé Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    RNA 14:524-34. 2008
  7. ncbi Biologically active molecules with a "light switch"
    Gunter Mayer
    Kekule Institut fur Organische Chemie und Biochemie, Rheinische Friedrich Wilhelms Universitat Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 45:4900-21. 2006
  8. doi RNA diagnostics: real-time RT-PCR strategies and promising novel target RNAs
    Gunter Mayer
    Institute, Rheinische Friedrich Wilhelms University of Bonn, Gerhard Domagk Strasse 1, Bonn, Germany
    Wiley Interdiscip Rev RNA 2:32-41. 2011
  9. ncbi Light-induced formation of G-quadruplex DNA secondary structures
    Gunter Mayer
    Universitat Bonn, Kekule Institut fur Organische Chemie und Biochemie, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Chembiochem 6:1966-70. 2005
  10. doi Aptamer-based modulation of blood coagulation
    G Mayer
    University of Bonn, Life and Medical Sciences Institute, Bonn, Germany
    Hamostaseologie 31:258-63. 2011

Collaborators

Detail Information

Publications42

  1. ncbi Light regulation of aptamer activity: an anti-thrombin aptamer with caged thymidine nucleobases
    Alexander Heckel
    Kekulé Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard Domagk Str 1, 53121 Bonn, Germany
    J Am Chem Soc 127:822-3. 2005
    ..With light (366 nm) the caging group can be removed, yielding the unmodified active aptamer...
  2. ncbi [Nucleic acids in the drug discovery process: aptamers as therapeutic and as active ingredients for drugs]
    Gunter Mayer
    Rheinische Friedrich Wilhelms Universitat Bonn, LIMES Institut, Programmeinheit Chemische Biologie und Medizinische Chemie, c o Kekulé Institut für Org Chemie und Biochemie, Gerhard Domagk Str 1, 53121 Bonn
    Pharm Unserer Zeit 36:432-6. 2007
    ..Die Aptamertechnologie eröffnet vielfältige Applikationsmöglichkeiten und neuere Arbeiten zeigen, dass sie auch bei der Identifikation niedermolekularer Wirkstoffe einen wertvollen Beitrag leisten kann...
  3. ncbi Differential regulation of protein subdomain activity with caged bivalent ligands
    Gunter Mayer
    Life and Medical Sciences, Program Unit Chemical Biology and Medicinal Chemistry, University of Bonn c o Kekulé Institute for Organic Chemistry and Biochemistry, Gerhard Domagk Strasse 1, Bonn, Germany
    Chembiochem 10:654-7. 2009
    ..To the best of our knowledge, this is the first study to show that it is possible to modulate individual domain activity in aptamers, and thus also domain activity in proteins, with light...
  4. ncbi Light-stimulated patterning of cells
    Gunter Mayer
    University of Bonn, LIMES Life and Medical Sciences, c o Kekulé Institute, Gerhard Domagk Strasse 1, Bonn, Germany
    Chembiochem 8:857-8. 2007
  5. doi The chemical biology of aptamers
    Gunter Mayer
    Life and Medical Sciences, Prog Unit Chemical Biology and Medicinal Chemistry, University of Bonn c o Kekulé Institute for Organic Chemistry and Biochemistry, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 48:2672-89. 2009
    ..This Review summarizes the most convenient approaches to their preparation and new developments in the field of aptamers. The application of aptamers in chemical biology is also discussed...
  6. pmc An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro
    Gunter Mayer
    Life and Medical Sciences Bonn, Program Unit Chemical Biology, c o Kekulé Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    RNA 14:524-34. 2008
    ..The aptamer is two orders of magnitude more potent than the best GRK2 inhibitors described previously and shows high selectivity for the GRK family of protein kinases...
  7. ncbi Biologically active molecules with a "light switch"
    Gunter Mayer
    Kekule Institut fur Organische Chemie und Biochemie, Rheinische Friedrich Wilhelms Universitat Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 45:4900-21. 2006
    ....
  8. doi RNA diagnostics: real-time RT-PCR strategies and promising novel target RNAs
    Gunter Mayer
    Institute, Rheinische Friedrich Wilhelms University of Bonn, Gerhard Domagk Strasse 1, Bonn, Germany
    Wiley Interdiscip Rev RNA 2:32-41. 2011
    ..Furthermore, the emerging use of microRNA and circulating RNA profiles complementing the broad spectrum of RNA diagnosis is discussed...
  9. ncbi Light-induced formation of G-quadruplex DNA secondary structures
    Gunter Mayer
    Universitat Bonn, Kekule Institut fur Organische Chemie und Biochemie, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Chembiochem 6:1966-70. 2005
  10. doi Aptamer-based modulation of blood coagulation
    G Mayer
    University of Bonn, Life and Medical Sciences Institute, Bonn, Germany
    Hamostaseologie 31:258-63. 2011
    ..In this review, we summarize recent developments in the design and evaluation of aptamers for anticoagulant therapy and research...
  11. doi Plug and play with RNA
    Gunter Mayer
    Life and Medical Sciences Institute, University of Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 50:12400-1. 2011
    ....
  12. ncbi Aptamers in research and drug development
    Gunter Mayer
    NascaCell IP GmbH, Munich, Germany
    BioDrugs 18:351-9. 2004
    ..Recent publications impressively confirm that aptamers can be used either as surrogate inhibitors for the identification of small molecule lead compounds or as biopharmaceuticals...
  13. ncbi An anticoagulant with light-triggered antidote activity
    Alexander Heckel
    Life and Medical Sciences, LIMES, Program Unit Chemical Biology and Medicinal Chemistry, Kekulé Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 45:6748-50. 2006
  14. doi Thrombin inhibition profiles in healthy individuals and thrombophilic patients
    Heiko Rühl
    Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Bonn, Germany
    Thromb Haemost 107:848-53. 2012
    ..In contrast, plasma levels of antithrombin falling below 80% of normal markedly prolong the thrombin half-life...
  15. doi Enrichment of cell-targeting and population-specific aptamers by fluorescence-activated cell sorting
    Marie Sophie L Raddatz
    Life and Medical Science, Universitat Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 47:5190-3. 2008
  16. doi Construction of DNA architectures with RNA hairpins
    Gunter Mayer
    Universitat Bonn, LIMES Life and Medical Science Institut, Programmeinheit Chemische Biologie und Medizinische Chemie, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 47:971-3. 2008
  17. ncbi Multidomain targeting generates a high-affinity thrombin-inhibiting bivalent aptamer
    Jens Muller
    Institute of Experimental Haematology and Transfusion Medicine, Universitat Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany
    Chembiochem 8:2223-6. 2007
  18. ncbi RNA ligands that distinguish metabolite-induced conformations in the TPP riboswitch
    Gunter Mayer
    Life and Medical Sciences LIMES, Program Unit Chemical Biology and Medicinal Chemistry c o Kekulé Institut für Organische Chemie und Biochemie, Universitat Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Angew Chem Int Ed Engl 46:557-60. 2007
  19. doi Aptamer modules as sensors and detectors
    Michael Famulok
    LIMES Institute, Chemical Biology and Medicinal Chemistry Unit, University of Bonn, 53121 Bonn, Germany
    Acc Chem Res 44:1349-58. 2011
    ..Progress in these areas will greatly expand the range of aptasensor applications, leading to enhanced diagnosis of diseases in clinical practice and, ultimately, improved patient care...
  20. doi In vitro selection of ssDNA aptamers using biotinylated target proteins
    Gunter Mayer
    University of Bonn, Bonn, Germany
    Methods Mol Biol 535:19-32. 2009
    ..This procedure allows the application of streptavidin-biotin chemistry to separate bound from unbound DNA species during the selection process...
  21. ncbi Screening assays to identify artificial glmS ribozyme activators
    Christina E Lünse
    Life and Medical Sciences Institute, University of Bonn, Bonn, Germany
    Methods Mol Biol 1103:199-209. 2014
    ....
  22. pmc Sensing complex regulatory networks by conformationally controlled hairpin ribozymes
    S Hani Najafi-Shoushtari
    Kekule Institut fur Organische Chemie und Biochemie, University of Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Nucleic Acids Res 32:3212-9. 2004
    ..This approach allows to precisely sense the activity status of a protein controlled by its metabolite molecule...
  23. doi Secondary structures and functional requirements for thiM riboswitches from Desulfovibrio vulgaris, Erwinia carotovora and Rhodobacter spheroides
    Andrea Rentmeister
    LIMES Institute, Program Unit Chemical Biology and Medicinal Chemistry, c o Kekulé Institute for Organic Chemistry and Biochemistry of the University of Bonn, Gerhard Domagk Strasse 1, D 53121 Bonn, Germany
    Biol Chem 389:127-34. 2008
    ..Our study shows that aptamer domains in riboswitches with high similarity in their secondary structures can communicate with a broad variety of non-related expression domains by similar mechanisms...
  24. doi Aptamer-guided caging for selective masking of protein domains
    Falk Rohrbach
    Life and Medical Sciences Institute, Gerhard Domagk Strasse 1, 53121 Bonn Germany http www mayerlab de
    Angew Chem Int Ed Engl 52:11912-5. 2013
    ..No structural knowledge of the protein site is needed. Thrombin Exosite I activity can be efficiently regulated with this approach, allowing light-controlled activation of blood clotting. ..
  25. doi Caged glucosamine-6-phosphate for the light-control of riboswitch activity
    Bernhard Wulffen
    University of Bonn, Life and Medical Sciences, c o Kekulé Institute for Organic Chemistry and Biochemistry, Bonn, Germany
    Photochem Photobiol Sci 11:489-92. 2012
    ..This glmS riboswitch maintains its activity when embedded in the 3'-untranslated region of eukaryotic mRNA molecules and caged GlcN6P reduces the amount of translated EGFP upon irradiation with light in vitro...
  26. doi ADLOC: an aptamer-displacement assay based on luminescent oxygen channeling
    Björn Niebel
    Life and Medical Sciences LIMES Institute, Chemical Biology and Medicinal Chemistry Unit, University of Bonn, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Chemistry 16:11100-7. 2010
    ..With full automation and the use of commercially available plate readers, we estimate that the ADLOC-based assay described here could be used to screen at least 100,000 compounds per day...
  27. ncbi Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography
    Jens Muller
    Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Sigmund Freud Str 25, 53127, Bonn, Germany
    Anal Bioanal Chem 390:1033-7. 2008
    ..We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences...
  28. ncbi Functional aptamers and aptazymes in biotechnology, diagnostics, and therapy
    Michael Famulok
    LIMES Institute, Program Unit Chemical Biology and Medicinal Chemistry, c o Kekulé Institut für Organische Chemie und Biochemie, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Chem Rev 107:3715-43. 2007
  29. ncbi Protamine-adsorbed magnetic nanoparticles for efficient isolation and concentration of hepatitis-C virus from human plasma samples
    Abdallah A Yassin
    Institute for Exp Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany
    Chem Commun (Camb) 50:590-2. 2014
    ....
  30. ncbi Intramers and aptamers: applications in protein-function analyses and potential for drug screening
    Michael Famulok
    Rheinische Friedrich Wilhelms Universitat Bonn, Kekule Institut fur Organische Chemie und Biochemie, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Chembiochem 6:19-26. 2005
  31. pmc Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation
    Marc Beyer
    Life and Medical Sciences Institute, Laboratory for Genomics and Immunoregulation, University of Bonn, Bonn, Germany
    Nat Immunol 12:898-907. 2011
    ..Our data support the proposal that inhibition of SATB1-mediated modulation of global chromatin remodeling is pivotal for maintaining T(reg) cell functionality...
  32. ncbi Chemical maturation of a bivalent aptamer by single domain variation
    Falk Rohrbach
    Life and Medical Sciences Institute, University of Bonn, Gerhard Domagk Strasse 1, 53121 Bonn Germany
    Chembiochem 13:631-4. 2012
    ....
  33. doi In vitro selection of conformational probes for riboswitches
    Gunter Mayer
    Rheinische Friedrich Wilhelms Universität Bonn Life and Medical Sciences Institute, Gerhard Domagk Str 1, 53121, Bonn, Germany
    Methods Mol Biol 540:291-300. 2009
    ..By this means, and after determination of the binding region, domains which are necessary for proper function of a full-length riboswitch can be identified...
  34. ncbi High-throughput-compatible assay for glmS riboswitch metabolite dependence
    Gunter Mayer
    Universitat Bonn, Kekule Institut fur Organische Chemie und Biochemie, Gerhard Domagk Strasse 1, 53121 Bonn, Germany
    Chembiochem 7:602-4. 2006
  35. ncbi Aptamer-based affinity labeling of proteins
    Jan L Vinkenborg
    University of Bonn, LIMES Institute, Bonn, Germany
    Angew Chem Int Ed Engl 51:9176-80. 2012
    ..The aptamer-protein complexes can be enriched in vitro, from a cellular lysate and from the surface of living cells, opening new ways to study aptamer interactions in biological contexts...
  36. doi Label-free impedimetric aptasensor for lysozyme detection based on carbon nanotube-modified screen-printed electrodes
    Falk Rohrbach
    LIMES Institute, Program Unit Chemical Biology and Medicinal Chemistry, University of Bonn, 53121 Bonn, Germany
    Anal Biochem 421:454-9. 2012
    ..5 mM [Fe(CN)₆]³⁻/⁴⁻. This assay system provides a means for the label-free, concentration-dependent, and selective detection of lysozyme with an observed detection limit of 12.09 μg/ml (equal to 862 nM)...
  37. pmc Conformational changes in the expression domain of the Escherichia coli thiM riboswitch
    Andrea Rentmeister
    LIMES Program Unit Chemical Biology and Medicinal Chemistry, Kekulé Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard Domagk Strasse 1, D 53121 Bonn, Germany
    Nucleic Acids Res 35:3713-22. 2007
    ..Furthermore, structural probing provides an explanation for the lack of genetic control exerted by a riboswitch variant with mutations in the expression domain that still binds TPP...
  38. pmc Multifunctional nucleic acids for tumor cell treatment
    Monika Pofahl
    1 Life and Medical Sciences Institute, University of Bonn, Bonn, Germany
    Nucleic Acid Ther 24:171-7. 2014
    ..These findings will have a strong impact on future developments regarding aptamer- and antimiR-related applications for tumor targeting and treatment. ..
  39. doi The promise of riboswitches as potential antibacterial drug targets
    Christina E Lünse
    Life and Medical Sciences Institute, University of Bonn, Gerhard Domagk Str 1, 53121 Bonn, Germany
    Int J Med Microbiol 304:79-92. 2014
    ..Here, we summarize the current inventory of riboswitch-targeting compounds, their characteristics and antibacterial potential. ..
  40. doi Cyplecksins are covalent inhibitors of the pleckstrin homology domain of cytohesin
    Mohamed Hussein
    Life and Medical Sciences LIMES Institute, Universitat Bonn, Gerhard Domagk Strasse 1, 53121 Bonn Germany http www famuloklab de
    Angew Chem Int Ed Engl 52:9529-33. 2013
    ..In cells, Cyplecksins interfere with the phosphoinositol-dependent membrane recruitment of cytohesins. Cyplecksins may be useful in validating cytohesins as potential drug targets. ..
  41. pmc Casein kinase 2 dependent phosphorylation of neprilysin regulates receptor tyrosine kinase signaling to Akt
    Martin Siepmann
    Department of Neurology, Molecular Cell Biology, University of Bonn, Bonn, Germany
    PLoS ONE 5:. 2010
    ..Thus, our data demonstrate a regulatory role of CK2 in the interaction of NEP with PTEN and insulin/IGF-1 signaling...
  42. doi Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures
    Gunter Mayer
    Department of Life and Medical Sciences, University of Bonn, Bonn, Germany
    Nat Protoc 5:1993-2004. 2010
    ....