G Lipps

Summary

Affiliation: University of Bayreuth
Country: Germany

Publications

  1. pmc Adenylosuccinate synthase from Saccharomyces cerevisiae: homologous overexpression, purification and characterization of the recombinant protein
    G Lipps
    University Bayreuth, Biochemistry II, Universitatsstrasse 30, D 95447 Bayreuth, Germany
    Biochem J 341:537-43. 1999
  2. doi request reprint Genetic tools for Sulfolobus spp.: vectors and first applications
    Silvia Berkner
    Department of Biochemistry, University of Bayreuth, Universitätsstr 30, 95447 Bayreuth, Germany
    Arch Microbiol 190:217-30. 2008
  3. pmc Properties of an unusual DNA primase from an archaeal plasmid
    Kirsten Beck
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Nucleic Acids Res 35:5635-45. 2007
  4. pmc Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea
    Silvia Berkner
    Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
    Nucleic Acids Res 35:e88. 2007
  5. ncbi request reprint Thermodynamic analysis of the single-stranded DNA binding activity of the archaeal replication protein A (RPA) from Sulfolobus solfataricus
    Uwe Kernchen
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Biochemistry 45:594-603. 2006
  6. ncbi request reprint Plasmids and viruses of the thermoacidophilic crenarchaeote Sulfolobus
    Georg Lipps
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95440, Bayreuth, Germany
    Extremophiles 10:17-28. 2006
  7. ncbi request reprint The replication protein of the Sulfolobus islandicus plasmid pRN1
    G Lipps
    Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Biochem Soc Trans 32:240-4. 2004
  8. ncbi request reprint Structure of a bifunctional DNA primase-polymerase
    Georg Lipps
    Institute of Biochemistry, University of Bayreuth, Universitatsstrasse 30, D 95447 Bayreuth, Germany
    Nat Struct Mol Biol 11:157-62. 2004
  9. pmc A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activity
    Georg Lipps
    Department of Biochemistry, University of Bayreuth, Universitatsstrasse 30, Germany
    EMBO J 22:2516-25. 2003
  10. pmc Thermostable and site-specific DNA binding of the gene product ORF56 from the Sulfolobus islandicus plasmid pRN1, a putative archael plasmid copy control protein
    G Lipps
    University of Bayreuth, Biochemistry II, Universitatsstrasse 30, D 95447 Bayreuth, Germany
    Nucleic Acids Res 29:904-13. 2001

Collaborators

  • P Cramer
  • K Sugasawa
  • Holger Stark
  • H Lilie
  • Silvia Berkner
  • Kirsten Beck
  • Gerhard Krauss
  • Martin Sanchez
  • Santina Prato
  • Stefan Söllner
  • Uwe Kernchen
  • Yiwei Huang
  • Rasa Beinoraviciute-Kellner
  • Markus Zeeb
  • Thomas Hey
  • T Hey
  • Alessandro Vannini
  • Markus Drechsler
  • Mose Rossi
  • Rosa Maria Vitale
  • Patrizia Contursi
  • Simonetta Bartolucci
  • Michele Saviano
  • Sonja Verena Albers
  • Dennis Grogan
  • Sylvain Cottaz
  • Hugues Driguez
  • Jochen Balbach
  • Fumio Hanaoka
  • Shigenori Iwai
  • G Krauss

Detail Information

Publications23

  1. pmc Adenylosuccinate synthase from Saccharomyces cerevisiae: homologous overexpression, purification and characterization of the recombinant protein
    G Lipps
    University Bayreuth, Biochemistry II, Universitatsstrasse 30, D 95447 Bayreuth, Germany
    Biochem J 341:537-43. 1999
    ..Another unusual feature of the enzyme from S. cerevisiae is its negligible inhibition by adenine nucleotides and its pronounced inhibition by Cl(-) ions...
  2. doi request reprint Genetic tools for Sulfolobus spp.: vectors and first applications
    Silvia Berkner
    Department of Biochemistry, University of Bayreuth, Universitätsstr 30, 95447 Bayreuth, Germany
    Arch Microbiol 190:217-30. 2008
    ..The advantages and disadvantages of the different systems are discussed and aims for further improvement of genetic systems are identified...
  3. pmc Properties of an unusual DNA primase from an archaeal plasmid
    Kirsten Beck
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Nucleic Acids Res 35:5635-45. 2007
    ..Primer synthesis starts outside of the recognition motif, immediately 5' to the recognition motif. The fidelity of the primase synthesis is high, as non-complementary bases are not incorporated into the primer...
  4. pmc Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea
    Silvia Berkner
    Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
    Nucleic Acids Res 35:e88. 2007
    ..The new vector system promises to facilitate genetic studies of Sulfolobus and to have biotechnological uses, such as the overexpression or optimization of thermophilic enzymes that are not readily performed in mesophilic hosts...
  5. ncbi request reprint Thermodynamic analysis of the single-stranded DNA binding activity of the archaeal replication protein A (RPA) from Sulfolobus solfataricus
    Uwe Kernchen
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Biochemistry 45:594-603. 2006
    ..The binding reaction is strongly exothermic with enthalpies of about -55 to -70 kJ/mol, depending upon the salt. Van't Hoff analysis suggests that the binding enthalpy is temperature independent...
  6. ncbi request reprint Plasmids and viruses of the thermoacidophilic crenarchaeote Sulfolobus
    Georg Lipps
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95440, Bayreuth, Germany
    Extremophiles 10:17-28. 2006
    ..Sulfolobus is one of the best-studied archaeons and could develop into an important model organism of the crenarchaea and the archaea...
  7. ncbi request reprint The replication protein of the Sulfolobus islandicus plasmid pRN1
    G Lipps
    Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Biochem Soc Trans 32:240-4. 2004
    ..This domain constitutes the first member of the DNA polymerase family E. Based on the biochemical characterization of the gene products a model of how pRN1 is replicated in vivo is proposed...
  8. ncbi request reprint Structure of a bifunctional DNA primase-polymerase
    Georg Lipps
    Institute of Biochemistry, University of Bayreuth, Universitatsstrasse 30, D 95447 Bayreuth, Germany
    Nat Struct Mol Biol 11:157-62. 2004
    ..We propose that archaeal and eukaryotic primases and the prim-pol domain have a common evolutionary ancestor, a bifunctional replicase for small DNA genomes...
  9. pmc A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activity
    Georg Lipps
    Department of Biochemistry, University of Bayreuth, Universitatsstrasse 30, Germany
    EMBO J 22:2516-25. 2003
    ..ORF904 constitutes a new type of replication enzyme, which could have evolved independently from the eubacterial and archaeal/eukaryal proteins of DNA replication...
  10. pmc Thermostable and site-specific DNA binding of the gene product ORF56 from the Sulfolobus islandicus plasmid pRN1, a putative archael plasmid copy control protein
    G Lipps
    University of Bayreuth, Biochemistry II, Universitatsstrasse 30, D 95447 Bayreuth, Germany
    Nucleic Acids Res 29:904-13. 2001
    ..2 kJ/mol. Furthermore, recombinant ORF56 proved to be highly thermostable and is able to bind DNA up to 85 degrees C...
  11. pmc The protein ORF80 from the acidophilic and thermophilic archaeon Sulfolobus islandicus binds highly site-specifically to double-stranded DNA and represents a novel type of basic leucine zipper protein
    G Lipps
    University Bayreuth, Biochemistry II, Universitatsstrasse 30, 95447 Bayreuth, Germany
    Nucleic Acids Res 29:4973-82. 2001
    ..Compared to all known basic leucine zipper proteins the order of the domains is reversed in ORF80. ORF80 may therefore constitute a new subclass of basic leucine zipper DNA-binding proteins...
  12. ncbi request reprint Binding of XPA and RPA to damaged DNA investigated by fluorescence anisotropy
    T Hey
    , , , 95447 Bayreuth, Germany
    Biochemistry 40:2901-10. 2001
    ..In contrast, cooperative binding of XPA and RPA is observed in EMSA. Our results point to a damage-sensing function of the XPA-RPA complex with RPA mediating the important DNA contacts...
  13. pmc The archaeo-eukaryotic primase of plasmid pRN1 requires a helix bundle domain for faithful primer synthesis
    Kirsten Beck
    Institute of Biochemistry, University of Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Germany
    Nucleic Acids Res 38:6707-18. 2010
    ..Furthermore, we identified mutants of the C-terminal domain, which are defective in template binding, dinucleotide formation and conformation change prior to DNA extension...
  14. pmc A highly acid-stable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus
    Yiwei Huang
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Biochem J 385:581-8. 2005
    ..A cellulolytic enzyme with such a combination of stability and activity at high temperatures and low pH has not been described so far and could be a valuable tool for the large-scale hydrolysis of cellulose under acidic conditions...
  15. ncbi request reprint Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus
    Stefan Söllner
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Extremophiles 10:629-34. 2006
    ..The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C...
  16. pmc DNA translocation activity of the multifunctional replication protein ORF904 from the archaeal plasmid pRN1
    Martin Sanchez
    Department of Biochemistry, University of Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Switzerland
    Nucleic Acids Res 37:6831-48. 2009
    ..In addition we propose that a winged helix DNA-binding domain at the C-terminus of the helicase domain could assist the binding of the replication protein specifically to the replication origin...
  17. pmc Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operon
    Silvia Berkner
    Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
    J Bacteriol 189:1711-21. 2007
    ..Reporter gene experiments demonstrated that the copy number control protein Orf56 represses transcription of the orf56-orf904 cotranscript in vivo...
  18. pmc An active nonautonomous mobile element in Sulfolobus islandicus REN1H1
    Silvia Berkner
    Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
    J Bacteriol 189:2145-9. 2007
    ..It does not contain terminal inverted repeats and transposes by a replicative mechanism. This newly discovered element has been named SMN1 (for Sulfolobus miniature noninverted repeat transposable element)...
  19. ncbi request reprint The XPC-HR23B complex displays high affinity and specificity for damaged DNA in a true-equilibrium fluorescence assay
    Thomas Hey
    Lehrstuhl für Biochemie, Universitat Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Germany
    Biochemistry 41:6583-7. 2002
    ..Upon addition of XPA to the XPC binding reaction mixtures, it was not possible to detect cooperative ternary complex formation on the platinated 36 bp probe...
  20. doi request reprint Molecular modeling and functional characterization of the monomeric primase-polymerase domain from the Sulfolobus solfataricus plasmid pIT3
    Santina Prato
    Dipartimento di Biologia Strutturale e Funzionale, Universita degli Studi di Napoli Federico II, Naples, Italy
    FEBS J 275:4389-402. 2008
    ..To the best of our knowledge, Rep245 typifies the shortest functional domain from a crenarchaeal plasmid endowed with DNA and RNA synthesis and terminal transferase activity...
  21. ncbi request reprint Folding and association of an extremely stable dimeric protein from Sulfolobus islandicus
    Markus Zeeb
    Laboratorium für Biochemie III, Universitat Bayreuth, D 95440 Bayreuth, Germany
    J Mol Biol 336:227-40. 2004
    ..0 x 10(7)M(-1)s(-1)) and unfolds extremely slowly (5.7 year(-1)). Therefore, high stability is coupled to a slow unfolding rate, which is often observed for proteins of extremophilic organisms...
  22. doi request reprint Mutation and reversion frequencies of different Sulfolobus species and strains
    Silvia Berkner
    Department of Biochemistry, University of Bayreuth, Universitätsstr 30, 95447 Bayreuth, Germany
    Extremophiles 12:263-70. 2008
    ..6-1.5 mutations per cell division and 10(8) cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed...
  23. ncbi request reprint In vitro selection of DNA binding sites for ABF1 protein from Saccharomyces cerevisiae
    Rasa Beinoraviciute-Kellner
    Universitat Bayreuth, Biochemie II, 95440 Bayreuth, Germany
    FEBS Lett 579:4535-40. 2005
    ..We found DNA sequences that are bound by ABF1 with nearly two-magnitude higher affinity as compared to the hitherto accepted ABF1 consensus sequence. This led us to postulate a strong recognition motive: 5'-TnnCGTnnnnnnTGAT-3'...