Research Topics
| G LippsSummaryAffiliation: University of Bayreuth Country: Germany Publications
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Detail Information
Publications
Adenylosuccinate synthase from Saccharomyces cerevisiae: homologous overexpression, purification and characterization of the recombinant proteinG Lipps
University Bayreuth, Biochemistry II, Universitatsstrasse 30, D 95447 Bayreuth, Germany
Biochem J 341:537-43. 1999..Another unusual feature of the enzyme from S. cerevisiae is its negligible inhibition by adenine nucleotides and its pronounced inhibition by Cl(-) ions...
Genetic tools for Sulfolobus spp.: vectors and first applicationsSilvia Berkner
Department of Biochemistry, University of Bayreuth, Universitätsstr 30, 95447 Bayreuth, Germany
Arch Microbiol 190:217-30. 2008..The advantages and disadvantages of the different systems are discussed and aims for further improvement of genetic systems are identified...
Properties of an unusual DNA primase from an archaeal plasmidKirsten Beck
Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
Nucleic Acids Res 35:5635-45. 2007..Primer synthesis starts outside of the recognition motif, immediately 5' to the recognition motif. The fidelity of the primase synthesis is high, as non-complementary bases are not incorporated into the primer...- Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaeaSilvia Berkner
Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
Nucleic Acids Res 35:e88. 2007..The new vector system promises to facilitate genetic studies of Sulfolobus and to have biotechnological uses, such as the overexpression or optimization of thermophilic enzymes that are not readily performed in mesophilic hosts... 
Thermodynamic analysis of the single-stranded DNA binding activity of the archaeal replication protein A (RPA) from Sulfolobus solfataricusUwe Kernchen
Institute of Biochemistry, University of Bayreuth, , 95447 Bayreuth, Germany
Biochemistry 45:594-603. 2006..The binding reaction is strongly exothermic with enthalpies of about -55 to -70 kJ/mol, depending upon the salt. Van't Hoff analysis suggests that the binding enthalpy is temperature independent...- Plasmids and viruses of the thermoacidophilic crenarchaeote SulfolobusGeorg Lipps
Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95440, Bayreuth, Germany
Extremophiles 10:17-28. 2006..Sulfolobus is one of the best-studied archaeons and could develop into an important model organism of the crenarchaea and the archaea... - The replication protein of the Sulfolobus islandicus plasmid pRN1G Lipps
Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
Biochem Soc Trans 32:240-4. 2004..This domain constitutes the first member of the DNA polymerase family E. Based on the biochemical characterization of the gene products a model of how pRN1 is replicated in vivo is proposed... - Structure of a bifunctional DNA primase-polymeraseGeorg Lipps
Institute of Biochemistry, University of Bayreuth, Universitatsstrasse 30, D 95447 Bayreuth, Germany
Nat Struct Mol Biol 11:157-62. 2004..We propose that archaeal and eukaryotic primases and the prim-pol domain have a common evolutionary ancestor, a bifunctional replicase for small DNA genomes... - A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activityGeorg Lipps
Department of Biochemistry, University of Bayreuth, Universitatsstrasse 30, Germany
EMBO J 22:2516-25. 2003..ORF904 constitutes a new type of replication enzyme, which could have evolved independently from the eubacterial and archaeal/eukaryal proteins of DNA replication... - Thermostable and site-specific DNA binding of the gene product ORF56 from the Sulfolobus islandicus plasmid pRN1, a putative archael plasmid copy control proteinG Lipps
University of Bayreuth, Biochemistry II, Universitatsstrasse 30, D 95447 Bayreuth, Germany
Nucleic Acids Res 29:904-13. 2001..2 kJ/mol. Furthermore, recombinant ORF56 proved to be highly thermostable and is able to bind DNA up to 85 degrees C... - The protein ORF80 from the acidophilic and thermophilic archaeon Sulfolobus islandicus binds highly site-specifically to double-stranded DNA and represents a novel type of basic leucine zipper proteinG Lipps
University Bayreuth, Biochemistry II, Universitatsstrasse 30, 95447 Bayreuth, Germany
Nucleic Acids Res 29:4973-82. 2001..Compared to all known basic leucine zipper proteins the order of the domains is reversed in ORF80. ORF80 may therefore constitute a new subclass of basic leucine zipper DNA-binding proteins... - Binding of XPA and RPA to damaged DNA investigated by fluorescence anisotropyT Hey
, , , 95447 Bayreuth, Germany
Biochemistry 40:2901-10. 2001..In contrast, cooperative binding of XPA and RPA is observed in EMSA. Our results point to a damage-sensing function of the XPA-RPA complex with RPA mediating the important DNA contacts... - The archaeo-eukaryotic primase of plasmid pRN1 requires a helix bundle domain for faithful primer synthesisKirsten Beck
Institute of Biochemistry, University of Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Germany
Nucleic Acids Res 38:6707-18. 2010..Furthermore, we identified mutants of the C-terminal domain, which are defective in template binding, dinucleotide formation and conformation change prior to DNA extension... - A highly acid-stable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricusYiwei Huang
Institute of Biochemistry, University of Bayreuth, , 95447 Bayreuth, Germany
Biochem J 385:581-8. 2005..A cellulolytic enzyme with such a combination of stability and activity at high temperatures and low pH has not been described so far and could be a valuable tool for the large-scale hydrolysis of cellulose under acidic conditions... - Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicusStefan Söllner
Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
Extremophiles 10:629-34. 2006..The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C... - DNA translocation activity of the multifunctional replication protein ORF904 from the archaeal plasmid pRN1Martin Sanchez
Department of Biochemistry, University of Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Switzerland
Nucleic Acids Res 37:6831-48. 2009..In addition we propose that a winged helix DNA-binding domain at the C-terminus of the helicase domain could assist the binding of the replication protein specifically to the replication origin... - Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operonSilvia Berkner
Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
J Bacteriol 189:1711-21. 2007..Reporter gene experiments demonstrated that the copy number control protein Orf56 represses transcription of the orf56-orf904 cotranscript in vivo... - An active nonautonomous mobile element in Sulfolobus islandicus REN1H1Silvia Berkner
Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
J Bacteriol 189:2145-9. 2007..It does not contain terminal inverted repeats and transposes by a replicative mechanism. This newly discovered element has been named SMN1 (for Sulfolobus miniature noninverted repeat transposable element)... - The XPC-HR23B complex displays high affinity and specificity for damaged DNA in a true-equilibrium fluorescence assayThomas Hey
, , , 95447 Bayreuth, Germany
Biochemistry 41:6583-7. 2002..Upon addition of XPA to the XPC binding reaction mixtures, it was not possible to detect cooperative ternary complex formation on the platinated 36 bp probe... - Molecular modeling and functional characterization of the monomeric primase-polymerase domain from the Sulfolobus solfataricus plasmid pIT3Santina Prato
Dipartimento di Biologia Strutturale e Funzionale, Universita degli Studi di Napoli Federico II, Naples, Italy
FEBS J 275:4389-402. 2008..To the best of our knowledge, Rep245 typifies the shortest functional domain from a crenarchaeal plasmid endowed with DNA and RNA synthesis and terminal transferase activity... - Folding and association of an extremely stable dimeric protein from Sulfolobus islandicusMarkus Zeeb
, , D-95440 Bayreuth, Germany
J Mol Biol 336:227-40. 2004..0 x 10(7)M(-1)s(-1)) and unfolds extremely slowly (5.7 year(-1)). Therefore, high stability is coupled to a slow unfolding rate, which is often observed for proteins of extremophilic organisms... - Mutation and reversion frequencies of different Sulfolobus species and strainsSilvia Berkner
Department of Biochemistry, University of Bayreuth, Universitätsstr 30, 95447 Bayreuth, Germany
Extremophiles 12:263-70. 2008..6-1.5 mutations per cell division and 10(8) cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed... - In vitro selection of DNA binding sites for ABF1 protein from Saccharomyces cerevisiaeRasa Beinoraviciute-Kellner
Universitat Bayreuth, Biochemie II, 95440 Bayreuth, Germany
FEBS Lett 579:4535-40. 2005..We found DNA sequences that are bound by ABF1 with nearly two-magnitude higher affinity as compared to the hitherto accepted ABF1 consensus sequence. This led us to postulate a strong recognition motive: 5'-TnnCGTnnnnnnTGAT-3'...