Research Topics
Genomes and Genes
| N InoueSummaryAffiliation: University of Heidelberg Country: Germany Publications
| Collaborators
|
Detail Information
Publications
Clostridium perfringens enterotoxin utilizes two structurally related membrane proteins as functional receptors in vivoJ Katahira
Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565, Japan
J Biol Chem 272:26652-8. 1997..These results indicate that CPE uses structurally related cellular proteins as functional receptors in vivo and that organs that have not so far been recognized as CPE-sensitive have the potential to be targets of CPE...- Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2R Watanabe
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
EMBO J 19:4402-11. 2000..These results reveal new essential and regulatory components of GPI-GnT and imply co-regulation of GPI-GnT and the dolichol-phosphate-mannose synthase that generates a mannosyl donor for GPI... - Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesisT Kinoshita
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, 565 0871, Osaka, Japan
Curr Opin Chem Biol 4:632-8. 2000..These studies have revealed the common and also different characteristics of glycosylphosphatidyl-inositol biosynthesis enzymes in different organisms, leading to the development of species-specific inhibitors of the pathway... - Requirement of PIG-F and PIG-O for transferring phosphoethanolamine to the third mannose in glycosylphosphatidylinositolY Hong
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
J Biol Chem 275:20911-9. 2000..Therefore, mammalian cells have redundant activities in transferring phosphoethanolamine to the third mannose, both of which require PIG-F... - The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1R Watanabe
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
EMBO J 17:877-85. 1998..These results suggest that GPI-GnT recognizes the fatty acyl chains of PI. The unusually complex organization of GPI-GnT may be relevant to selective usage of PI and/or regulation... - Molecular cloning and functional characterization of the receptor for Clostridium perfringens enterotoxinJ Katahira
Department of Bacterial Toxicology, Osaka University, Japan
J Cell Biol 136:1239-47. 1997..From these results, it is proposed that the enterotoxin receptor is required for both target cell recognition and pore formation in the cell membrane... - A homologue of Saccharomyces cerevisiae Dpm1p is not sufficient for synthesis of dolichol-phosphate-mannose in mammalian cellsS Tomita
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
J Biol Chem 273:9249-54. 1998..Therefore, in contrast to yeast, mammalian cells require hDPM1/mDPM1 protein and a product of another gene that is defective in Lec15 mutant cells for synthesis of Dol-P-Man... - PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8K Ohishi
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
EMBO J 20:4088-98. 2001..Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively... - Structures and chromosomal localizations of the glycosylphosphatidylinositol synthesis gene PIGC and its pseudogene PIGCP1Y Hong
Department of Immunoregulation, Osaka University, Japan
Genomics 44:347-9. 1997..PIGC is an intronless gene. We found an intronless pseudogene of PIGC, PIGCP1, and mapped it to chromosome 11p12-p13. The presence of a processed pseudogene is a common feature of PIGA, PIGF, and PIGC... - Human and mouse GPAA1 (Glycosylphosphatidylinositol anchor attachment 1) genes: genomic structures, chromosome loci and the presence of a minor class intronN Inoue
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
Cytogenet Cell Genet 84:199-205. 1999..The mouse GPAA1 gene (Gpaa1) bearing AG at the 3' splice site prepared by site-directed mutagenesis was functional, indicating that any nucleotide is allowed at the 3' end of a minor class intron... - Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteinsK Ohishi
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
Mol Biol Cell 11:1523-33. 2000..Moreover, Gaa1p and Gpi8p are associated with each other. Therefore, Gaa1p and Gpi8p constitute a GPI transamidase and cooperate in generating a carbonyl intermediate, a prerequisite for GPI attachment... - PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchorM Takahashi
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
EMBO J 15:4254-61. 1996..This autosomal location would explain why PIG-B is not involved in the defective GPI anchor synthesis in paroxysmal nocturnal hemoglobinuria, which is always caused by a somatic mutation of the X-linked PIG-A gene... - Expression cloning of PIG-L, a candidate N-acetylglucosaminyl-phosphatidylinositol deacetylaseN Nakamura
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565, Japan
J Biol Chem 272:15834-40. 1997..This orientation of PIG-L protein is consistent with the notion that the second step of GPI anchor biosynthesis occurs on the cytoplasmic side of the endoplasmic reticulum... - Bordetella bronchiseptica dermonecrotizing toxin induces reorganization of actin stress fibers through deamidation of Gln-63 of the GTP-binding protein RhoY Horiguchi
Department of Bacterial Toxicology, Research Institute for Microbial Diseases, Osaka University, 3 1, Yamada oka, Suita, Osaka 565, Japan
Proc Natl Acad Sci U S A 94:11623-6. 1997..These results indicate that the toxin catalyzes deamidation of Gln-63 of Rho and renders it constitutively active, leading to formation of actin stress fibers... - PIG-A and PIG-H, which participate in glycosylphosphatidylinositol anchor biosynthesis, form a protein complex in the endoplasmic reticulumR Watanabe
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565, Japan
J Biol Chem 271:26868-75. 1996..Taken together with the cytoplasmic orientation of GlcNAc-PI, these results indicated that PIG-A and PIG-H are subunits of the GPI GlcNAc transferase that transfers GlcNAc to PI on the cytoplasmic side of the ER... - PIG-C, one of the three human genes involved in the first step of glycosylphosphatidylinositol biosynthesis is a homologue of Saccharomyces cerevisiae GPI2N Inoue
Department of Immunoregulation, Osaka University, Japan
Biochem Biophys Res Commun 226:193-9. 1996..Since there are several human EST sequences that have homology to GPI1, our results suggest that four genes are involved in the first step of GPI anchor synthesis in mammalian cells... - Association of functional GITR gene polymorphisms related to expression of glucocorticoid-induced tumour necrosis factor-receptor (GITR) molecules with prognosis of autoimmune thyroid diseaseR Tomizawa
Department of Biomedical Informatics, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
Clin Exp Immunol 165:141-7. 2011..In conclusion, the rs3753348 C/G SNP in the GITR is associated with HD prognosis and expression on T(reg) and T(eff) cells... - Cloning and characterization of the murine GPI anchor synthesis gene Pigf, a homologue of the human PIGF geneK Ohishi
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, 565, Japan
Genomics 34:340-6. 1996..The results also extend a region in murine distal chromosome 17 that is syntenic to human chromosome 2p16-p22... - Structure and chromosomal localization of the GPI-anchor synthesis gene PIGF and its pseudogene psi PIGFK Ohishi
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
Genomics 29:804-7. 1995..We also identified the processed pseudogene of PIGF (psi PIGF) and mapped it to 5q35... - Expression cloning of genes for GPI-anchor biosynthesisT Kinoshita
Department of Immunoregulation, Osaka University, Japan
Braz J Med Biol Res 27:127-32. 1994..PIG-B cDNA restored the surface expression of Thy-1 on SIA-b cells and also synthesis of mature type GPI-anchor precursors in these cells.(ABSTRACT TRUNCATED AT 250 WORDS).. - The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesisT Miyata
Department of Immunoregulation, Osaka University, Japan
Science 259:1318-20. 1993..PIG-A was necessary for synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis... - Age-related decreases in the response of aquaporin-5 to acetylcholine in rat parotid glandsN Inoue
Department of Pharmacology, Tokushima University School of Dentistry, Kuramoto-cho, Tokushima 770-8504, Japan
J Dent Res 82:476-80. 2003..These results suggest that SNI-2011 might have therapeutic benefit for the treatment of age-related xerostomia... - Three soluble form messages of murine CD46 are produced through alternative mRNA splicingA Tsujimura
Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka 537-8511, Japan
J Biochem 130:841-8. 2001.... - Molecular cloning and functional characterization of chicken toll-like receptors. A single chicken toll covers multiple molecular patternsA Fukui
Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Nakamichi, Higashinari ku, Osaka 537 8511, Japan
J Biol Chem 276:47143-9. 2001..In oviparous animals, the duplicated TLRs in the pattern-recognition system may function for host-pathogen discrimination in a manner that is distinct from that in mammals... - Involvement of NADH/NADPH oxidase in human platelet ROS productionT Seno
Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan
Thromb Res 103:399-409. 2001..Thus, human platelets have the enzymatic activity of p22(phox)-based NADH/NADPH oxidase, and this oxidase is likely one of the important sources of ROS in platelets... - A 14-3-3 protein homologue is expressed in feline enteroepithelial-stages of Toxoplasma gondiiT Koyama
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
Vet Parasitol 96:65-74. 2001..These findings suggest that T. gondii parasites produce a protein showing partial homology with members of the 14-3-3 protein family and this protein is expressed in feline enteroepithelial-stages parasites... - A novel chicken membrane-associated complement regulatory protein: molecular cloning and functional characterizationN Inoue
Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
J Immunol 166:424-31. 2001..These results suggested that even in nonmammals a C regulatory membrane protein with ubiquitous tissue distribution should be a prerequisite for protection of host cells from homologous C attack... - Characterization of genomic PIG-A gene: a gene for glycosylphosphatidylinositol-anchor biosynthesis and paroxysmal nocturnal hemoglobinuriaY Iida
Department of Internal Medicine, Branch Hospital, Nagoya University School of Medicine, Japan
Blood 83:3126-31. 1994..We report pairs of oligonucleotide primers for polymerase chain reaction that should be useful to amplify and analyze various regions of the PIG-A gene in patients with PNH... - Transcription and initiation of ColE1 DNA replication in Escherichia coli K-12N Inoue
Institute of Medical Science, University of Tokyo, Japan
J Bacteriol 173:1208-14. 1991....