Ed Hurt

Summary

Affiliation: University of Heidelberg
Country: Germany

Publications

  1. pmc Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing
    Olivier Gadal
    BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 157:941-51. 2002
  2. pmc Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p
    S M Bailer
    University of Heidelberg, Biochemie Zentrum Heidelberg BZH, Germany
    EMBO J 17:1107-19. 1998
  3. ncbi request reprint Mex67p mediates nuclear export of a variety of RNA polymerase II transcripts
    E Hurt
    University of Heidelberg, BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Biol Chem 275:8361-8. 2000
  4. pmc Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export
    K Strasser
    BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 150:695-706. 2000
  5. pmc Structure and assembly of the Nup84p complex
    S Siniossoglou
    Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 149:41-54. 2000
  6. pmc Mutational uncoupling of the role of Sus1 in nuclear pore complex targeting of an mRNA export complex and histone H2B deubiquitination
    Christoph Klöckner
    Biochemistry Center BZH, Heidelberg University, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Biol Chem 284:12049-56. 2009
  7. pmc Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Cell 141:606-17. 2010
  8. ncbi request reprint Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit
    Thorsten Schäfer
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nature 441:651-5. 2006
  9. pmc The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores
    Tamas Fischer
    Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, Germany
    EMBO J 21:5843-52. 2002
  10. ncbi request reprint Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits
    Kyriaki Galani
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Biol Chem 279:55411-8. 2004

Collaborators

Detail Information

Publications56

  1. pmc Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing
    Olivier Gadal
    BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 157:941-51. 2002
    ..All together, these data suggest that pre-rRNA cleavage at site C2 specifically requires Rlp7p and occurs within pre-60S particles located in the GC region of the nucleolus...
  2. pmc Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p
    S M Bailer
    University of Heidelberg, Biochemie Zentrum Heidelberg BZH, Germany
    EMBO J 17:1107-19. 1998
    ..Thus, the in vivo function of Gle2p is strictly coupled to the short GLEBS within Nup116p which links this putative mRNA transport factor to the nuclear pores...
  3. ncbi request reprint Mex67p mediates nuclear export of a variety of RNA polymerase II transcripts
    E Hurt
    University of Heidelberg, BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Biol Chem 275:8361-8. 2000
    ..Thus, Mex67p is an mRNA export factor for a broad range of polymerase II transcripts...
  4. pmc Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export
    K Strasser
    BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 150:695-706. 2000
    ....
  5. pmc Structure and assembly of the Nup84p complex
    S Siniossoglou
    Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 149:41-54. 2000
    ..Thus, the Nup84p complex constitutes a paradigm of an NPC structural module with distinct composition, structure, and a role in nuclear mRNA export and NPC bio- genesis...
  6. pmc Mutational uncoupling of the role of Sus1 in nuclear pore complex targeting of an mRNA export complex and histone H2B deubiquitination
    Christoph Klöckner
    Biochemistry Center BZH, Heidelberg University, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Biol Chem 284:12049-56. 2009
    ..e. Sac3) to the nuclear pore complexes and exhibited nuclear mRNA export defects. This study has implications for how Sus1, in combination with distinct interaction partners, can regulate diverse aspects of gene expression...
  7. pmc Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Cell 141:606-17. 2010
    ..Our structural and functional analyses reveal a central role of Sgf11 and Sgf73 in activating Ubp8 for deubiquitinating histone H2B and demonstrate how a DUB can be allosterically regulated by its nonsubstrate partners...
  8. ncbi request reprint Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit
    Thorsten Schäfer
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nature 441:651-5. 2006
    ..In vivo depletion of Hrr25 inhibits growth and leads to the accumulation of immature 40S subunits that contain unstably bound Rps3. We conclude that the kinase activity of Hrr25 regulates the maturation of 40S ribosomal subunits...
  9. pmc The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores
    Tamas Fischer
    Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, Germany
    EMBO J 21:5843-52. 2002
    ..Significantly, mutations in Sac3p or Thp1p lead to strong mRNA export defects. Taken together, our data suggest that the novel Sac3p-Thp1p complex functions by docking the mRNP to specific nucleoporins at the nuclear entrance of the NPC...
  10. ncbi request reprint Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits
    Kyriaki Galani
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Biol Chem 279:55411-8. 2004
    ..Using an in vivo 60 S subunit export assay, a strong accumulation of the large subunit reporter Rpl25-GFP (green fluorescent protein) in the nucleus and at the nuclear periphery was seen in rea1 mutants at restrictive conditions...
  11. pmc The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
    Stefanie E Grund
    Biochemie Zentrum der Universitat Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 182:897-910. 2008
    ..Our data show that the inner nuclear membrane protein Src1 functions at the interface between subtelomeric gene expression and TREX-dependent messenger RNA export through the nuclear pore complexes...
  12. ncbi request reprint Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery
    Susana Rodriguez-Navarro
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Cell 116:75-86. 2004
    ..Finally, mRNA export is impaired in sus1 mutants. These data provide an unexpected connection between the SAGA histone acetylase complex and the mRNA export machinery...
  13. pmc The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes
    Thorsten Schäfer
    BZH, Universitat Heidelberg, Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO J 22:1370-80. 2003
    ..Our data provide an initial biochemical map of the pre-40S ribosomal subunit on its path from the nucleolus to the cytoplasm. This pathway involves fewer changes in composition than seen during 60S biogenesis...
  14. ncbi request reprint Formation and nuclear export of preribosomes are functionally linked to the small-ubiquitin-related modifier pathway
    Vikram Govind Panse
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Traffic 7:1311-21. 2006
    ..Together our data suggest that sumoylation of preribosomal particles in the nucleus and subsequent desumoylation at the NPC is necessary for efficient ribosome biogenesis and export in eukaryotes...
  15. ncbi request reprint TREX is a conserved complex coupling transcription with messenger RNA export
    Katja Strasser
    BZH, University of Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Nature 417:304-8. 2002
    ..Our data indicate that the TREX complex has a conserved role in coupling transcription to mRNA export...
  16. ncbi request reprint Arx1 functions as an unorthodox nuclear export receptor for the 60S preribosomal subunit
    Bettina Bradatsch
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Mol Cell 27:767-79. 2007
    ..In contrast, the predicted central cavity of Arx1 is involved in the interaction with FG repeat nucleoporins and 60S subunit export. Thus, an ancient enzyme fold has been adopted by Arx1 to function as a nuclear export receptor...
  17. ncbi request reprint Molecular basis for the functional interaction of dynein light chain with the nuclear-pore complex
    Philipp Stelter
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Nat Cell Biol 9:788-96. 2007
    ..Thus, it is possible that dynein light chain plays a role in organizing natively unfolded Phe-Gly repeats within the NPC scaffold to facilitate nucleocytoplasmic transport...
  18. pmc Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export
    Yoshitaka Matsuo
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, Heidelberg D 69120, Germany
    Nature 505:112-6. 2014
    ..We conclude that Nug2 is a regulatory GTPase that monitors pre-60S maturation, with release from its placeholder site linked to recruitment of the nuclear export machinery. ..
  19. pmc Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex
    Ed Hurt
    Biochemie Zentrum Heidelberg BZH, University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 101:1858-62. 2004
    ..We conclude that recruitment of the SR-like proteins Gbp2 and Hrb1 to mRNA occurs cotranscriptionally by means of association with the TREX complex and/or Ctk1...
  20. ncbi request reprint Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery
    Tamas Fischer
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Nat Cell Biol 6:840-8. 2004
    ..A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery...
  21. doi request reprint Synchronizing nuclear import of ribosomal proteins with ribosome assembly
    Dieter Kressler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, Heidelberg D 69120, Germany
    Science 338:666-71. 2012
    ..Symportin-mediated coimport of Rpl5-Rpl11 could ensure coordinated and stoichiometric incorporation of these proteins into pre-60S ribosomes...
  22. ncbi request reprint Nuclear export of ribosomal 60S subunits by the general mRNA export receptor Mex67-Mtr2
    Wei Yao
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Mol Cell 26:51-62. 2007
    ..Thus, the general mRNA exporter Mex67-Mtr2 contains a distinct electrostatic interaction surface for transporting 60S preribosomal cargo...
  23. pmc 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm
    Tracy A Nissan
    Biochemie Zentrum Heidelberg, Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    EMBO J 21:5539-47. 2002
    ..As the 60S pre-ribosome reaches the cytoplasm, almost all of the factors are dissociated. These data provide an initial biochemical map of 60S ribosomal subunit formation on its path from the nucleolus to the cytoplasm...
  24. pmc The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly
    Jochen Bassler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Mol Cell 38:712-21. 2010
    ....
  25. pmc Two structurally distinct domains of the nucleoporin Nup170 cooperate to tether a subset of nucleoporins to nuclear pores
    Dirk Flemming
    Centre for Biochemistry, University of Heidelberg, Heidelberg, Germany
    J Cell Biol 185:387-95. 2009
    ..We conclude that Nup170 performs its role as a structural adapter between cytoplasmically oriented Nups and the nuclear pore membrane...
  26. ncbi request reprint A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits
    Tracy A Nissan
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120, Germany
    Mol Cell 15:295-301. 2004
    ..Rea1 and the Rix1 complex could mediate ATP-dependent remodeling of 60S subunits and subsequent export from the nucleoplasm to the cytoplasm...
  27. ncbi request reprint The tRNA aminoacylation co-factor Arc1p is excluded from the nucleus by an Xpo1p-dependent mechanism
    Kyriaki Galani
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    FEBS Lett 579:969-75. 2005
    ..Thus, the cytoplasmic location of Arc1p is maintained by Xpo1p-dependent nuclear export and Arc1p could act as an adapter in the nucleocytoplasmic trafficking of tRNA and/or the tRNA-aminoacylation machinery...
  28. ncbi request reprint A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunits
    Philipp Milkereit
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, Germany
    J Biol Chem 278:4072-81. 2003
    ..Thus, Noc4p and Nop14p form a novel Noc complex with a specific role in nucleolar 40 S subunit formation and subsequent export to the cytoplasm...
  29. pmc Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p
    Silvia Neumann
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO Rep 4:1156-62. 2003
    ..These data implicate ubiquitination as a mechanism coordinating the major nuclear RNA biogenesis pathways...
  30. pmc RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1
    Brigitte Pertschy
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Biol Chem 284:35079-91. 2009
    ..In ltv1/pfa1 mutants the 20 S pre-rRNA was susceptible to 3' to 5' degradation by the cytoplasmic exosome. This degraded into the 3' region of the 18 S rRNA, strongly indicating that the preribosomes are structurally defective...
  31. ncbi request reprint Driving ribosome assembly
    Dieter Kressler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Biochim Biophys Acta 1803:673-83. 2010
    ..This review summarizes our current knowledge on eukaryotic ribosome assembly, with particular focus on the molecular role of the involved energy-consuming enzymes...
  32. pmc The AAA ATPase Rix7 powers progression of ribosome biogenesis by stripping Nsa1 from pre-60S particles
    Dieter Kressler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Cell Biol 181:935-44. 2008
    ..Altogether, our data suggest that Rix7 is required for the release of Nsa1 from a discrete preribosomal particle, thereby triggering the progression of 60S ribosome biogenesis...
  33. doi request reprint Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits
    Cornelia Ulbrich
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Cell 138:911-22. 2009
    ..Thus, Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit...
  34. pmc Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure
    Julien Batisse
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 307, D 69120 Heidelberg, Germany
    J Biol Chem 284:34911-7. 2009
    ..Electron microscopy revealed that many of the mRNPs have a characteristic elongated structure. Our data suggest that mRNPs, although associated with different mRNAs, have a unifying core structure...
  35. ncbi request reprint Pre-ribosomes on the road from the nucleolus to the cytoplasm
    Herbert Tschochner
    Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Trends Cell Biol 13:255-63. 2003
    ..The future challenge will be to assign functions to the more than 150 non-ribosomal factors that transiently associate with the developing pre-ribosomes...
  36. doi request reprint Analysis of the yeast nucleoporin Nup188 reveals a conserved S-like structure with similarity to karyopherins
    Dirk Flemming
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Struct Biol 177:99-105. 2012
    ..Using bioinformatic methods, we have generated a pseudo-atomic structural model of Nup188 and its related Nup192, which further strengthens the view that the large α-solenoid structural Nups are related to karyopherins...
  37. ncbi request reprint Identification and characterization of a novel RanGTP-binding protein in the yeast Saccharomyces cerevisiae
    Andreas Braunwarth
    Biochemie Zentrum Heidelberg, Ruprecht Karls Universitat, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Biol Chem 278:15397-405. 2003
    ..Based on these results and the exclusive conservation of the protein in the fungal kingdom, we hypothesize that Yrb30p represents a novel modulator of the Ran GTPase switch related to fungal lifestyle...
  38. ncbi request reprint Coordinated nuclear import of RNA polymerase III subunits
    Ulrike Hardeland
    Biochemie Zentrum der Universitat Heidelberg, INF328, D 69120 Heidelberg, Germany
    Traffic 7:465-73. 2006
    ..We expect these studies to be a starting point to dissect the complex biogenesis pathway of eukaryotic RNA polymerases...
  39. doi request reprint Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Cell Biol 10:707-15. 2008
    ..Loss of Sgf73 from SAGA abrogates gene gating of GAL1 and causes a GAL1 mRNA export defect. Thus, Sgf73 provides a molecular scaffold to integrate the regulation of H2B ubiquitin levels, tethering of a gene to the NPC and export of mRNA...
  40. doi request reprint Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile
    Stefan Amlacher
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, Heidelberg D 69120, Germany
    Cell 146:277-89. 2011
    ..This assembly illustrates how Nup interactions can generate structural plasticity within the NPC scaffold. Our findings therefore demonstrate the utility of the genome of a thermophilic eukaryote for studying complex molecular machines...
  41. ncbi request reprint A proteome-wide approach identifies sumoylated substrate proteins in yeast
    Vikram Govind Panse
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany and Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 279:41346-51. 2004
    ..Thus, the identification of a broad range of SUMO-1 substrate proteins is expected to lead to further insight into the regulatory aspects of sumoylation...
  42. doi request reprint Protein interfaces of the conserved Nup84 complex from Chaetomium thermophilum shown by crosslinking mass spectrometry and electron microscopy
    Karsten Thierbach
    Biochemie Zentrum der Universitat Heidelberg, INF328, D 69120 Heidelberg, Germany
    Structure 21:1672-82. 2013
    ..Thus, the thermophilic Nup84 complex can serve as a structural model for higher eukaryotic Nup107-Nup160 assemblies to gain insight into its possible configuration within the NPC scaffold...
  43. pmc Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins
    Malik Lutzmann
    BZH Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO J 21:387-97. 2002
    ..Taken together, our data document that the Nup84p-Nup133p complex self-assembles in a modular concept from distinct smaller nucleoporin construction sets...
  44. doi request reprint Monitoring spatiotemporal biogenesis of macromolecular assemblies by pulse-chase epitope labeling
    Philipp Stelter
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Mol Cell 47:788-96. 2012
    ..Thus, we provide an innovative strategy to study dynamic protein assembly within cellular networks...
  45. pmc Membrane curvature induced by Arf1-GTP is essential for vesicle formation
    Rainer Beck
    Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 105:11731-6. 2008
    ..Strikingly, this mutant was not able to deform membranes, suggesting that GTP-induced dimerization of Arf1 is a critical step inducing membrane curvature during the formation of coated vesicles...
  46. ncbi request reprint Reconstitution of Nup157 and Nup145N into the Nup84 complex
    Malik Lutzmann
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Biol Chem 280:18442-51. 2005
    ..Nup157 is a hollow sphere that resembles a clamp or a gripping hand. Thus, we could reconstitute an interaction between a large structural Nup, an FG repeat Nup, and a major structural module of the nuclear pore complex...
  47. ncbi request reprint The crystal structure of Ebp1 reveals a methionine aminopeptidase fold as binding platform for multiple interactions
    Eva Kowalinski
    Heidelberg University Biochemistry Center BZH, INF 328, D 69120 Heidelberg, Germany
    FEBS Lett 581:4450-4. 2007
    ..The structure provides insights in how Ebp1 discriminates between its different interaction partners...
  48. ncbi request reprint Unconventional tethering of Ulp1 to the transport channel of the nuclear pore complex by karyopherins
    Vikram Govind Panse
    BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Nat Cell Biol 5:21-7. 2003
    ..This location could allow Ulp1 to remove SUMO-1 from sumoylated cargo proteins during their passage through the nuclear pore channel...
  49. pmc Structural insights into tail-anchored protein binding and membrane insertion by Get3
    Gunes Bozkurt
    Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 106:21131-6. 2009
    ..We postulate that ATP hydrolysis is needed to release Get3 from its receptor. Taken together, our results provide mechanistic insights into posttranslational targeting of TA membrane proteins by Get3...
  50. ncbi request reprint Eukaryotic ribosome biogenesis at a glance
    Emma Thomson
    Biochemistry Center BZH, University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Cell Sci 126:4815-21. 2013
    ..In this Cell Science at a Glance article and the accompanying poster, we summarise the current knowledge on eukaryotic ribosome biogenesis, with an emphasis on the yeast model system. ..
  51. pmc An intron in the YRA1 gene is required to control Yra1 protein expression and mRNA export in yeast
    Susana Rodriguez-Navarro
    BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    EMBO Rep 3:438-42. 2002
    ..Thus, an intron in YRA1 controls Yra1p expression and mRNA export...
  52. ncbi request reprint Exporting RNA from the nucleus to the cytoplasm
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:761-73. 2007
    ..Export of mRNAs is unique as it is extensively coupled to transcription (in yeast) and splicing (in metazoa). Understanding the mechanisms that connect RNP formation with export is a major challenge in the field...
  53. pmc Probing the nucleoporin FG repeat network defines structural and functional features of the nuclear pore complex
    Philipp Stelter
    Biochemie Zentrum der Universitat Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 195:183-92. 2011
    ..Our method allows us to specifically probe FG Nups in vivo, which provides insight into the organization and function of the NPC transport channel...
  54. ncbi request reprint Nuclear export of tRNA
    George Simos
    Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Results Probl Cell Differ 35:115-31. 2002
  55. doi request reprint Gene regulation by nucleoporins and links to cancer
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Mol Cell 38:6-15. 2010
    ..This review outlines new mechanistic links between nucleoporin function and cancer pathogenesis...
  56. pmc ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit
    Sébastien Ferreira-Cerca
    Biochemistry Center, University of Heidelberg, Heidelberg, Germany
    Nat Struct Mol Biol 19:1316-23. 2012
    ..We propose that phosphoryl transfer from ATP to Asp257 in Rio2's active site and subsequent hydrolysis of the aspartylphosphate could be a trigger to power late cytoplasmic 40S subunit biogenesis...