Research Topics
Genomes and Genes | Ed HurtSummaryAffiliation: University of Heidelberg Country: Germany Publications
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Publications
Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2pS M Bailer
University of Heidelberg, Biochemie Zentrum Heidelberg BZH, Germany
EMBO J 17:1107-19. 1998..Thus, the in vivo function of Gle2p is strictly coupled to the short GLEBS within Nup116p which links this putative mRNA transport factor to the nuclear pores...
Mex67p mediates nuclear export of a variety of RNA polymerase II transcriptsE Hurt
University of Heidelberg, BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
J Biol Chem 275:8361-8. 2000..Thus, Mex67p is an mRNA export factor for a broad range of polymerase II transcripts...
Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA exportK Strasser
BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
J Cell Biol 150:695-706. 2000....- Structure and assembly of the Nup84p complexS Siniossoglou
Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
J Cell Biol 149:41-54. 2000..Thus, the Nup84p complex constitutes a paradigm of an NPC structural module with distinct composition, structure, and a role in nuclear mRNA export and NPC bio- genesis... - Mutational uncoupling of the role of Sus1 in nuclear pore complex targeting of an mRNA export complex and histone H2B deubiquitinationChristoph Klöckner
Biochemistry Center BZH, Heidelberg University, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
J Biol Chem 284:12049-56. 2009..e. Sac3) to the nuclear pore complexes and exhibited nuclear mRNA export defects. This study has implications for how Sus1, in combination with distinct interaction partners, can regulate diverse aspects of gene expression... - Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase moduleAlwin Köhler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Cell 141:606-17. 2010..Our structural and functional analyses reveal a central role of Sgf11 and Sgf73 in activating Ubp8 for deubiquitinating histone H2B and demonstrate how a DUB can be allosterically regulated by its nonsubstrate partners... - Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunitThorsten Schäfer
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Nature 441:651-5. 2006..In vivo depletion of Hrr25 inhibits growth and leads to the accumulation of immature 40S subunits that contain unstably bound Rps3. We conclude that the kinase activity of Hrr25 regulates the maturation of 40S ribosomal subunits... - Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunitsKyriaki Galani
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Biol Chem 279:55411-8. 2004..Using an in vivo 60 S subunit export assay, a strong accumulation of the large subunit reporter Rpl25-GFP (green fluorescent protein) in the nucleus and at the nuclear periphery was seen in rea1 mutants at restrictive conditions... - The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear poresTamas Fischer
Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, Germany
EMBO J 21:5843-52. 2002..Significantly, mutations in Sac3p or Thp1p lead to strong mRNA export defects. Taken together, our data suggest that the novel Sac3p-Thp1p complex functions by docking the mRNP to specific nucleoporins at the nuclear entrance of the NPC... - The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expressionStefanie E Grund
Biochemie Zentrum der Universitat Heidelberg, D 69120 Heidelberg, Germany
J Cell Biol 182:897-910. 2008..Our data show that the inner nuclear membrane protein Src1 functions at the interface between subtelomeric gene expression and TREX-dependent messenger RNA export through the nuclear pore complexes... - Formation and nuclear export of preribosomes are functionally linked to the small-ubiquitin-related modifier pathwayVikram Govind Panse
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Traffic 7:1311-21. 2006..Together our data suggest that sumoylation of preribosomal particles in the nucleus and subsequent desumoylation at the NPC is necessary for efficient ribosome biogenesis and export in eukaryotes... - The path from nucleolar 90S to cytoplasmic 40S pre-ribosomesThorsten Schäfer
BZH, Universitat Heidelberg, Neuenheimer Feld 328, D 69120 Heidelberg, Germany
EMBO J 22:1370-80. 2003..Our data provide an initial biochemical map of the pre-40S ribosomal subunit on its path from the nucleolus to the cytoplasm. This pathway involves fewer changes in composition than seen during 60S biogenesis... - Yeast centrin Cdc31 is linked to the nuclear mRNA export machineryTamas Fischer
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Nat Cell Biol 6:840-8. 2004..A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery... - Arx1 functions as an unorthodox nuclear export receptor for the 60S preribosomal subunitBettina Bradatsch
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Mol Cell 27:767-79. 2007..In contrast, the predicted central cavity of Arx1 is involved in the interaction with FG repeat nucleoporins and 60S subunit export. Thus, an ancient enzyme fold has been adopted by Arx1 to function as a nuclear export receptor... 
Synchronizing nuclear import of ribosomal proteins with ribosome assemblyDieter Kressler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, Heidelberg D 69120, Germany
Science 338:666-71. 2012..Symportin-mediated coimport of Rpl5-Rpl11 could ensure coordinated and stoichiometric incorporation of these proteins into pre-60S ribosomes...- Nuclear export of ribosomal 60S subunits by the general mRNA export receptor Mex67-Mtr2Wei Yao
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Mol Cell 26:51-62. 2007..Thus, the general mRNA exporter Mex67-Mtr2 contains a distinct electrostatic interaction surface for transporting 60S preribosomal cargo... - Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinerySusana Rodriguez-Navarro
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Cell 116:75-86. 2004..Finally, mRNA export is impaired in sus1 mutants. These data provide an unexpected connection between the SAGA histone acetylase complex and the mRNA export machinery... - Molecular basis for the functional interaction of dynein light chain with the nuclear-pore complexPhilipp Stelter
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Nat Cell Biol 9:788-96. 2007..Thus, it is possible that dynein light chain plays a role in organizing natively unfolded Phe-Gly repeats within the NPC scaffold to facilitate nucleocytoplasmic transport... - TREX is a conserved complex coupling transcription with messenger RNA exportKatja Strasser
BZH, University of Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Nature 417:304-8. 2002..Our data indicate that the TREX complex has a conserved role in coupling transcription to mRNA export... - Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complexEd Hurt
Biochemie-Zentrum Heidelberg (BZH, University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 101:1858-62. 2004..We conclude that recruitment of the SR-like proteins Gbp2 and Hrb1 to mRNA occurs cotranscriptionally by means of association with the TREX complex and/or Ctk1... - The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assemblyJochen Bassler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Mol Cell 38:712-21. 2010.... - Two structurally distinct domains of the nucleoporin Nup170 cooperate to tether a subset of nucleoporins to nuclear poresDirk Flemming
Centre for Biochemistry, University of Heidelberg, Heidelberg, Germany
J Cell Biol 185:387-95. 2009..We conclude that Nup170 performs its role as a structural adapter between cytoplasmically oriented Nups and the nuclear pore membrane... - A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunitsTracy A Nissan
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120, Germany
Mol Cell 15:295-301. 2004..Rea1 and the Rix1 complex could mediate ATP-dependent remodeling of 60S subunits and subsequent export from the nucleoplasm to the cytoplasm... - A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunitsPhilipp Milkereit
Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, Germany
J Biol Chem 278:4072-81. 2003..Thus, Noc4p and Nop14p form a novel Noc complex with a specific role in nucleolar 40 S subunit formation and subsequent export to the cytoplasm... - Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5pSilvia Neumann
Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
EMBO Rep 4:1156-62. 2003..These data implicate ubiquitination as a mechanism coordinating the major nuclear RNA biogenesis pathways... - RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1Brigitte Pertschy
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Biol Chem 284:35079-91. 2009..In ltv1/pfa1 mutants the 20 S pre-rRNA was susceptible to 3' to 5' degradation by the cytoplasmic exosome. This degraded into the 3' region of the 18 S rRNA, strongly indicating that the preribosomes are structurally defective... - 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasmTracy A Nissan
Biochemie-Zentrum Heidelberg, , Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
EMBO J 21:5539-47. 2002..As the 60S pre-ribosome reaches the cytoplasm, almost all of the factors are dissociated. These data provide an initial biochemical map of 60S ribosomal subunit formation on its path from the nucleolus to the cytoplasm... - The AAA ATPase Rix7 powers progression of ribosome biogenesis by stripping Nsa1 from pre-60S particlesDieter Kressler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
J Cell Biol 181:935-44. 2008..Altogether, our data suggest that Rix7 is required for the release of Nsa1 from a discrete preribosomal particle, thereby triggering the progression of 60S ribosome biogenesis... - Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structureJulien Batisse
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 307, D 69120 Heidelberg, Germany
J Biol Chem 284:34911-7. 2009..Electron microscopy revealed that many of the mRNPs have a characteristic elongated structure. Our data suggest that mRNPs, although associated with different mRNAs, have a unifying core structure... - Driving ribosome assemblyDieter Kressler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Biochim Biophys Acta 1803:673-83. 2010..This review summarizes our current knowledge on eukaryotic ribosome assembly, with particular focus on the molecular role of the involved energy-consuming enzymes... - Analysis of the yeast nucleoporin Nup188 reveals a conserved S-like structure with similarity to karyopherinsDirk Flemming
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
J Struct Biol 177:99-105. 2012..Using bioinformatic methods, we have generated a pseudo-atomic structural model of Nup188 and its related Nup192, which further strengthens the view that the large α-solenoid structural Nups are related to karyopherins... - Pre-ribosomes on the road from the nucleolus to the cytoplasmHerbert Tschochner
Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Trends Cell Biol 13:255-63. 2003..The future challenge will be to assign functions to the more than 150 non-ribosomal factors that transiently associate with the developing pre-ribosomes... - Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunitsCornelia Ulbrich
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Cell 138:911-22. 2009..Thus, Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit... - The tRNA aminoacylation co-factor Arc1p is excluded from the nucleus by an Xpo1p-dependent mechanismKyriaki Galani
Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
FEBS Lett 579:969-75. 2005..Thus, the cytoplasmic location of Arc1p is maintained by Xpo1p-dependent nuclear export and Arc1p could act as an adapter in the nucleocytoplasmic trafficking of tRNA and/or the tRNA-aminoacylation machinery... - Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA exportAlwin Köhler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Nat Cell Biol 10:707-15. 2008..Loss of Sgf73 from SAGA abrogates gene gating of GAL1 and causes a GAL1 mRNA export defect. Thus, Sgf73 provides a molecular scaffold to integrate the regulation of H2B ubiquitin levels, tethering of a gene to the NPC and export of mRNA... - A proteome-wide approach identifies sumoylated substrate proteins in yeastVikram Govind Panse
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany and Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
J Biol Chem 279:41346-51. 2004..Thus, the identification of a broad range of SUMO-1 substrate proteins is expected to lead to further insight into the regulatory aspects of sumoylation... - Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophileStefan Amlacher
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, Heidelberg D 69120, Germany
Cell 146:277-89. 2011..This assembly illustrates how Nup interactions can generate structural plasticity within the NPC scaffold. Our findings therefore demonstrate the utility of the genome of a thermophilic eukaryote for studying complex molecular machines... - Membrane curvature induced by Arf1-GTP is essential for vesicle formationRainer Beck
Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 105:11731-6. 2008..Strikingly, this mutant was not able to deform membranes, suggesting that GTP-induced dimerization of Arf1 is a critical step inducing membrane curvature during the formation of coated vesicles... - Identification and characterization of a novel RanGTP-binding protein in the yeast Saccharomyces cerevisiaeAndreas Braunwarth
Biochemie Zentrum Heidelberg, Ruprecht Karls Universitat, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Biol Chem 278:15397-405. 2003..Based on these results and the exclusive conservation of the protein in the fungal kingdom, we hypothesize that Yrb30p represents a novel modulator of the Ran GTPase switch related to fungal lifestyle... - Structural insights into tail-anchored protein binding and membrane insertion by Get3Gunes Bozkurt
Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 106:21131-6. 2009..We postulate that ATP hydrolysis is needed to release Get3 from its receptor. Taken together, our results provide mechanistic insights into posttranslational targeting of TA membrane proteins by Get3... - Unconventional tethering of Ulp1 to the transport channel of the nuclear pore complex by karyopherinsVikram Govind Panse
BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
Nat Cell Biol 5:21-7. 2003..This location could allow Ulp1 to remove SUMO-1 from sumoylated cargo proteins during their passage through the nuclear pore channel... - Probing the nucleoporin FG repeat network defines structural and functional features of the nuclear pore complexPhilipp Stelter
Biochemie Zentrum der Universitat Heidelberg, D 69120 Heidelberg, Germany
J Cell Biol 195:183-92. 2011..Our method allows us to specifically probe FG Nups in vivo, which provides insight into the organization and function of the NPC transport channel... - Reconstitution of Nup157 and Nup145N into the Nup84 complexMalik Lutzmann
, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Biol Chem 280:18442-51. 2005..Nup157 is a hollow sphere that resembles a clamp or a gripping hand. Thus, we could reconstitute an interaction between a large structural Nup, an FG repeat Nup, and a major structural module of the nuclear pore complex... - Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processingOlivier Gadal
BZH, Biochemie-Zentrum Heidelberg, D-69120 Heidelberg, Germany
J Cell Biol 157:941-51. 2002..All together, these data suggest that pre-rRNA cleavage at site C2 specifically requires Rlp7p and occurs within pre-60S particles located in the GC region of the nucleolus... - Exporting RNA from the nucleus to the cytoplasmAlwin Köhler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Nat Rev Mol Cell Biol 8:761-73. 2007..Export of mRNAs is unique as it is extensively coupled to transcription (in yeast) and splicing (in metazoa). Understanding the mechanisms that connect RNP formation with export is a major challenge in the field... - The crystal structure of Ebp1 reveals a methionine aminopeptidase fold as binding platform for multiple interactionsEva Kowalinski
Heidelberg University Biochemistry Center BZH, INF 328, D 69120 Heidelberg, Germany
FEBS Lett 581:4450-4. 2007..The structure provides insights in how Ebp1 discriminates between its different interaction partners... - Gene regulation by nucleoporins and links to cancerAlwin Köhler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Mol Cell 38:6-15. 2010..This review outlines new mechanistic links between nucleoporin function and cancer pathogenesis... - An intron in the YRA1 gene is required to control Yra1 protein expression and mRNA export in yeastSusana Rodriguez-Navarro
BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
EMBO Rep 3:438-42. 2002..Thus, an intron in YRA1 controls Yra1p expression and mRNA export... - Nuclear export of tRNAGeorge Simos
Biochemie-Zentrum Heidelberg (BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Results Probl Cell Differ 35:115-31. 2002 - Monitoring spatiotemporal biogenesis of macromolecular assemblies by pulse-chase epitope labelingPhilipp Stelter
Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Mol Cell 47:788-96. 2012..Thus, we provide an innovative strategy to study dynamic protein assembly within cellular networks... - Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporinsMalik Lutzmann
BZH Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
EMBO J 21:387-97. 2002..Taken together, our data document that the Nup84p-Nup133p complex self-assembles in a modular concept from distinct smaller nucleoporin construction sets...