Jonathan C Howard

Summary

Affiliation: University of Cologne
Country: Germany

Publications

  1. ncbi request reprint IIGP1, an interferon-gamma-inducible 47-kDa GTPase of the mouse, showing cooperative enzymatic activity and GTP-dependent multimerization
    Revathy C Uthaiah
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, 50674 Cologne, Germany
    J Biol Chem 278:29336-43. 2003
  2. ncbi request reprint A giant GTPase, very large inducible GTPase-1, is inducible by IFNs
    Thorsten Klamp
    Institute for Genetics, University of Cologne, Cologne, Germany
    J Immunol 171:1255-65. 2003
  3. pmc Disruption of Toxoplasma gondii parasitophorous vacuoles by the mouse p47-resistance GTPases
    Sascha Martens
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS Pathog 1:e24. 2005
  4. ncbi request reprint Evolution of immunity and pathogens
    Jonathan C Howard
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, Cologne, Germany
    Eur J Immunol 37:1721-3. 2007
  5. ncbi request reprint Introduction: cell-autonomous immunity
    Jonathan C Howard
    Institute for Genetics, University of Cologne, Zuelpicher Strasse 47, 50674 Cologne, Germany
    Microbes Infect 9:1633-5. 2007
  6. pmc Coordinated loading of IRG resistance GTPases on to the Toxoplasma gondii parasitophorous vacuole
    Aliaksandr Khaminets
    Institute for Genetics, University of Cologne, Zulpicher Strasse, Cologne 50674, Germany
    Cell Microbiol 12:939-61. 2010
  7. pmc Inactive and active states of the interferon-inducible resistance GTPase, Irga6, in vivo
    Natasa Papic
    Department of Cell Genetics, Institute for Genetics, University of Cologne, 50674 Cologne, Germany
    J Biol Chem 283:32143-51. 2008
  8. pmc Disruption of the Toxoplasma gondii parasitophorous vacuole by IFNgamma-inducible immunity-related GTPases (IRG proteins) triggers necrotic cell death
    Yang O Zhao
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS Pathog 5:e1000288. 2009
  9. pmc Localisation and mislocalisation of the interferon-inducible immunity-related GTPase, Irgm1 (LRG-47) in mouse cells
    Yang O Zhao
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS ONE 5:e8648. 2010
  10. pmc Phosphorylation of mouse immunity-related GTPase (IRG) resistance proteins is an evasion strategy for virulent Toxoplasma gondii
    Tobias Steinfeldt
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS Biol 8:e1000576. 2010

Collaborators

Detail Information

Publications28

  1. ncbi request reprint IIGP1, an interferon-gamma-inducible 47-kDa GTPase of the mouse, showing cooperative enzymatic activity and GTP-dependent multimerization
    Revathy C Uthaiah
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, 50674 Cologne, Germany
    J Biol Chem 278:29336-43. 2003
    ..The present analysis provides essential parameters to understand the molecular mechanism by which IIGP1 participates in this complex resistance program...
  2. ncbi request reprint A giant GTPase, very large inducible GTPase-1, is inducible by IFNs
    Thorsten Klamp
    Institute for Genetics, University of Cologne, Cologne, Germany
    J Immunol 171:1255-65. 2003
    ..In addition, we provide evidence that VLIG-1 is polymorphic in mice of different genetic backgrounds and is a member of a small gene family on mouse chromosome 7 with a conserved homologue located on human chromosome 11...
  3. pmc Disruption of Toxoplasma gondii parasitophorous vacuoles by the mouse p47-resistance GTPases
    Sascha Martens
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS Pathog 1:e24. 2005
    ..Targeted deletion of the IIGP1 gene results in partial loss of the IFN-gamma-mediated T. gondii growth restriction in mouse astrocytes...
  4. ncbi request reprint Evolution of immunity and pathogens
    Jonathan C Howard
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, Cologne, Germany
    Eur J Immunol 37:1721-3. 2007
    ..The Cologne Spring Meeting of the Institute of Genetics focuses on a different topic each year. In Spring 2007, the meeting focused on the complex evolutionary relationships between hosts and pathogens...
  5. ncbi request reprint Introduction: cell-autonomous immunity
    Jonathan C Howard
    Institute for Genetics, University of Cologne, Zuelpicher Strasse 47, 50674 Cologne, Germany
    Microbes Infect 9:1633-5. 2007
    ..Only a few cell-autonomous resistance mechanisms have been analysed in any depth and there is much more work to be done...
  6. pmc Coordinated loading of IRG resistance GTPases on to the Toxoplasma gondii parasitophorous vacuole
    Aliaksandr Khaminets
    Institute for Genetics, University of Cologne, Zulpicher Strasse, Cologne 50674, Germany
    Cell Microbiol 12:939-61. 2010
    ..The complex cooperative behaviour of IRG proteins in resisting Toxoplasma may hint at undiscovered complexity also in virulence mechanisms...
  7. pmc Inactive and active states of the interferon-inducible resistance GTPase, Irga6, in vivo
    Natasa Papic
    Department of Cell Genetics, Institute for Genetics, University of Cologne, 50674 Cologne, Germany
    J Biol Chem 283:32143-51. 2008
    ....
  8. pmc Disruption of the Toxoplasma gondii parasitophorous vacuole by IFNgamma-inducible immunity-related GTPases (IRG proteins) triggers necrotic cell death
    Yang O Zhao
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS Pathog 5:e1000288. 2009
    ..We conclude that IRG proteins resist infection by avirulent T. gondii by a novel mechanism involving disruption of the vacuolar membrane, which in turn ultimately leads to the necrotic death of the infected cell...
  9. pmc Localisation and mislocalisation of the interferon-inducible immunity-related GTPase, Irgm1 (LRG-47) in mouse cells
    Yang O Zhao
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS ONE 5:e8648. 2010
    ..In future analyses it should be borne in mind that tagging of Irgm1 leads to loss of Golgi localisation and enhanced localisation on endolysosomal membranes, probably as a result of constitutive activation...
  10. pmc Phosphorylation of mouse immunity-related GTPase (IRG) resistance proteins is an evasion strategy for virulent Toxoplasma gondii
    Tobias Steinfeldt
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS Biol 8:e1000576. 2010
    ..gondii and the mouse are widespread and abundant in the wild and are well-established model species with excellent analytical tools available...
  11. pmc A Toxoplasma gondii pseudokinase inhibits host IRG resistance proteins
    Martin C Fleckenstein
    Institute for Genetics, University of Cologne, Cologne, Germany
    PLoS Biol 10:e1001358. 2012
    ..This represents a novel mechanism in which a pseudokinase facilitates the phosphorylation of a target by a partner kinase by preparing the substrate for phosphorylation, rather than by upregulation of the activity of the kinase itself...
  12. pmc Regulatory interactions between IRG resistance GTPases in the cellular response to Toxoplasma gondii
    Julia P Hunn
    Department of Cell Genetics, Institute for Genetics, University of Cologne, Cologne, Germany
    EMBO J 27:2495-509. 2008
    ..Accumulation of IRG proteins at the PVM has previously been shown to be associated with a block in pathogen replication: our results relate for the first time the enzymatic properties of IRG proteins to their role in pathogen resistance...
  13. pmc A dedicated promoter drives constitutive expression of the cell-autonomous immune resistance GTPase, Irga6 (IIGP1) in mouse liver
    Jia Zeng
    Institute for Genetics of the University of Cologne, Cologne, Germany
    PLoS ONE 4:e6787. 2009
    ..In general, immune effector molecules are induced by infection...
  14. pmc The IFN-γ-inducible GTPase, Irga6, protects mice against Toxoplasma gondii but not against Plasmodium berghei and some other intracellular pathogens
    Oliver Liesenfeld
    Institute of Microbiology and Hygiene, Charite Universitatsmedizin Berlin, Berlin, Germany
    PLoS ONE 6:e20568. 2011
    ..Taken together, our results implicate Irga6 in the control of infection with T. gondii and further highlight the importance of the IRG system for resistance to this pathogen...
  15. pmc Why didn't Darwin discover Mendel's laws?
    Jonathan C Howard
    Institute for Genetics, University of Cologne, Germany
    J Biol 8:15. 2009
    ..Darwin's focus on small quantitative variations as the raw material of evolution may have prevented him from discovering the laws of inheritance...
  16. doi request reprint The IRG protein-based resistance mechanism in mice and its relation to virulence in Toxoplasma gondii
    Jonathan C Howard
    Institute for Genetics, University of Cologne, Zülpicher Strasse 47a, 50674 Cologne, Germany
    Curr Opin Microbiol 14:414-21. 2011
    ..Thus these two mechanisms form the two poles of a dynamic host-pathogen virulence-resistance relationship with interesting and accessible properties...
  17. doi request reprint The IRG proteins: a function in search of a mechanism
    Jonathan Howard
    Institute for Genetics, University of Cologne, Zuelpicher Strasse 47, 50674 Cologne, Germany
    Immunobiology 213:367-75. 2008
    ..Failure of hemopoietic stem cells during infection is associated with targeted deletion of one IRG protein, Irgm1. The significance of this non-cell-autonomous phenotype is discussed...
  18. pmc The activation mechanism of Irga6, an interferon-inducible GTPase contributing to mouse resistance against Toxoplasma gondii
    Nikolaus Pawlowski
    Institute for Genetics, Department of Cell Genetics, University of Cologne, Zülpicher Strasse 47a, 50674 Cologne, Germany
    BMC Biol 9:7. 2011
    ....
  19. ncbi request reprint Mechanisms regulating the positioning of mouse p47 resistance GTPases LRG-47 and IIGP1 on cellular membranes: retargeting to plasma membrane induced by phagocytosis
    Sascha Martens
    Institute for Genetics, University of Cologne, Cologne, Germany
    J Immunol 173:2594-606. 2004
    ..Thus, the p47 GTPases are a diverse battery of intracellular defense factors dynamically associated with different membrane systems...
  20. ncbi request reprint Cell-autonomous immunity to Toxoplasma gondii in mouse and man
    Stephanie Könen-Waisman
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, 50674 Cologne, Germany
    Microbes Infect 9:1652-61. 2007
    ..There are many poorly understood host and pathogen variables that affect the outcome of infection...
  21. pmc The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage
    Cemalettin Bekpen
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, 50674 Cologne, Germany
    Genome Biol 6:R92. 2005
    ..Surprisingly, there are no reports of p47 function in humans...
  22. pmc Irgm1 (LRG-47), a regulator of cell-autonomous immunity, does not localize to mycobacterial or listerial phagosomes in IFN-γ-induced mouse cells
    Helen M Springer
    Institute for Genetics, University of Cologne, 50674 Cologne, Germany
    J Immunol 191:1765-74. 2013
    ..We discuss these findings in the context of evidence that Irgm1 functions as a negative regulator of other members of the immunity-related GTPase protein family. ..
  23. pmc The immunity-related GTPases in mammals: a fast-evolving cell-autonomous resistance system against intracellular pathogens
    Julia P Hunn
    Institute for Genetics, University of Cologne, Zuelpicher Strasse 47a, Cologne, Germany
    Mamm Genome 22:43-54. 2011
    ..IRG proteins are targets for polymorphic T. gondii virulence factors, and genetic variation in the IRG system between different mouse strains correlates with resistance and susceptibility to virulent T. gondii strains...
  24. pmc Spontaneous focal activation of invariant natural killer T (iNKT) cells in mouse liver and kidney
    Jia Zeng
    Institute for Genetics, University of Cologne, Zuelpicher Strasse 47a, 50674 Cologne, Germany
    BMC Biol 8:142. 2010
    ..It is generally agreed that the immune phenotype of iNKT cells is maintained by repeated activation in peripheral tissues although no explicit evidence for such iNKT cell activity in vivo has so far been reported...
  25. ncbi request reprint Chlamydia muridarum evades growth restriction by the IFN-gamma-inducible host resistance factor Irgb10
    Jorn Coers
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    J Immunol 180:6237-45. 2008
    ..muridarum. These data suggest that C. muridarum has evolved a mechanism to escape the murine IFN-gamma response by restricting access of Irgb10 and possibly other IRG proteins to the inclusion...
  26. ncbi request reprint MHC class II region encoding proteins related to the multidrug resistance family of transmembrane transporters. 1990
    Edward V Deverson
    Department of Immunology, Institute of Animal Physiology and Genetics, Cambridge Research Station, Cambridge, UK
    J Immunol 180:2729-32. 2008
  27. ncbi request reprint Differential inductions of TNF-alpha and IGTP, IIGP by structurally diverse classic and non-classic lipopolysaccharides
    Nicolas Lapaque
    Centre d Immunologie INSERM CNRS Université Méditerranée, Case 906, 13288 Marseille, Cedex 9, France
    Cell Microbiol 8:401-13. 2006
    ..They also reinforce the hypothesis that non-classical lipid As act as virulence factors by favouring the escape from the innate immune system...