Veit Hornung

Summary

Affiliation: University of Bonn
Country: Germany

Publications

  1. pmc Of inflammasomes and pathogens--sensing of microbes by the inflammasome
    Franz Bauernfeind
    Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Germany
    EMBO Mol Med 5:814-26. 2013
  2. pmc AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC
    Veit Hornung
    Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 458:514-8. 2009
  3. ncbi request reprint Intracellular DNA recognition
    Veit Hornung
    Institute for Clinical Chemistry and Pharmacology, University Hospital, University of Bonn, Bonn, Germany
    Nat Rev Immunol 10:123-30. 2010
  4. pmc Listeria monocytogenes is sensed by the NLRP3 and AIM2 inflammasome
    Sarah Kim
    Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Eur J Immunol 40:1545-51. 2010
  5. pmc The AIM2 inflammasome is essential for host defense against cytosolic bacteria and DNA viruses
    Vijay A K Rathinam
    Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Nat Immunol 11:395-402. 2010
  6. doi request reprint Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP
    Andrea Ablasser
    Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53127 Bonn, Germany
    Nature 503:530-4. 2013
  7. ncbi request reprint cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING
    Andrea Ablasser
    Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53127 Bonn, Germany
    Nature 498:380-4. 2013
  8. pmc NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals
    Peter Duewell
    Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 464:1357-61. 2010
  9. ncbi request reprint The NLRP3/ASC/Caspase-1 axis regulates IL-1β processing in neutrophils
    Arun K Mankan
    Institute for Clinical Chemistry and Clinical Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Eur J Immunol 42:710-5. 2012
  10. pmc Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization
    Veit Hornung
    Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Immunol 9:847-56. 2008

Collaborators

Detail Information

Publications35

  1. pmc Of inflammasomes and pathogens--sensing of microbes by the inflammasome
    Franz Bauernfeind
    Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Germany
    EMBO Mol Med 5:814-26. 2013
    ..Further, we review how inflammasomes contribute to the anti microbial host defense by cytokine-dependent and cell autonomous mechanisms...
  2. pmc AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC
    Veit Hornung
    Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 458:514-8. 2009
    ..Collectively, these observations identify AIM2 as a new receptor for cytoplasmic DNA, which forms an inflammasome with the ligand and ASC to activate caspase-1...
  3. ncbi request reprint Intracellular DNA recognition
    Veit Hornung
    Institute for Clinical Chemistry and Pharmacology, University Hospital, University of Bonn, Bonn, Germany
    Nat Rev Immunol 10:123-30. 2010
    ..DNA can be detected in different cellular compartments and can induce a range of cellular responses, such as an antiviral response and pyroptotic cell death together with the maturation and release of active interleukin-1beta...
  4. pmc Listeria monocytogenes is sensed by the NLRP3 and AIM2 inflammasome
    Sarah Kim
    Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Eur J Immunol 40:1545-51. 2010
    ..monocytogenes. Altogether, these data establish a role for DNA sensing through the AIM2 inflammasome in the detection of intracellularly replicating bacteria...
  5. pmc The AIM2 inflammasome is essential for host defense against cytosolic bacteria and DNA viruses
    Vijay A K Rathinam
    Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Nat Immunol 11:395-402. 2010
    ..Collectively, our observations demonstrate the importance of AIM2 in the sensing of both bacterial and viral pathogens and in triggering innate immunity...
  6. doi request reprint Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP
    Andrea Ablasser
    Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53127 Bonn, Germany
    Nature 503:530-4. 2013
    ..Collectively, these observations identify cGAS-triggered cGAMP(2'-5') transfer as a novel host strategy that serves to rapidly convey antiviral immunity in a transcription-independent, horizontal manner. ..
  7. ncbi request reprint cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING
    Andrea Ablasser
    Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53127 Bonn, Germany
    Nature 498:380-4. 2013
    ..This 13-membered ring structure defines a novel class of second messenger molecules, extending the family of 2'-5'-linked antiviral biomolecules...
  8. pmc NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals
    Peter Duewell
    Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 464:1357-61. 2010
    ..These findings provide new insights into the pathogenesis of atherosclerosis and indicate new potential molecular targets for the therapy of this disease...
  9. ncbi request reprint The NLRP3/ASC/Caspase-1 axis regulates IL-1β processing in neutrophils
    Arun K Mankan
    Institute for Clinical Chemistry and Clinical Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Eur J Immunol 42:710-5. 2012
    ..Our findings establish the NLRP3 inflammasome as a key step in the secretion of matured IL-1β by neutrophils...
  10. pmc Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization
    Veit Hornung
    Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Immunol 9:847-56. 2008
    ..Our results indicate that the NALP3 inflammasome senses lysosomal damage as an endogenous 'danger' signal...
  11. pmc Cutting edge: NF-kappaB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression
    Franz G Bauernfeind
    Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Immunol 183:787-91. 2009
    ..Signals provided by NF-kappaB activators are necessary but not sufficient for NLRP3 activation, and a second stimulus such as ATP or crystal-induced damage is required for NLRP3 activation...
  12. pmc Cutting edge: reactive oxygen species inhibitors block priming, but not activation, of the NLRP3 inflammasome
    Franz Bauernfeind
    Unit for Clinical Biochemistry, Institute for Clinical Chemistry and Pharmacology, University Hospital, University of Bonn, 53127 Bonn, Germany
    J Immunol 187:613-7. 2011
    ..Although these data do not exclude a general role for ROS production in the process of NLRP3-triggered inflammation, they would put ROS upstream of NLRP3 induction, but not activation...
  13. pmc RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate
    Andrea Ablasser
    Institute for Clinical Chemistry and Pharmacology, University of Bonn, Bonn, Germany
    Nat Immunol 10:1065-72. 2009
    ..This pathway was important in the sensing of Epstein-Barr virus-encoded small RNAs, which were transcribed by RNA polymerase III and then triggered RIG-I activation. Thus, RNA polymerase III and RIG-I are pivotal in sensing viral DNA...
  14. ncbi request reprint Self-priming determines high type I IFN production by plasmacytoid dendritic cells
    Sarah Kim
    Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, Bonn, Germany
    Eur J Immunol 44:807-18. 2014
    ..Altogether, these data demonstrate that high type I IFN production by pDCs cannot be simply ascribed to cell-autonomous mechanisms, yet critically depends on the local immune context. ..
  15. pmc RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells
    Cristina Amparo Hagmann
    Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, University of Bonn, Bonn, Germany
    PLoS ONE 8:e62872. 2013
    ..Our results show that detection of Listeria RNA by RIG-I represents a non-redundant cytosolic immunorecognition pathway in non-immune cells lacking a functional STING dependent signaling pathway...
  16. ncbi request reprint Critical role of nucleotide-binding oligomerization domain-like receptor 3 in vascular repair
    Sebastian Schlaweck
    Department of Medicine Cardiology, University of Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany
    Biochem Biophys Res Commun 411:627-31. 2011
    ..In all, NLRP3 plays a non-redundant role in vascular damage mediated neointima formation. Our data establish NLRP3 as a key player in the response to vascular damage, which could open new avenues to therapeutic intervention...
  17. doi request reprint Inflammasomes: current understanding and open questions
    Franz Bauernfeind
    Unit for Clinical Biochemistry, Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, Sigmund Freud Strasse 25, Bonn, Germany
    Cell Mol Life Sci 68:765-83. 2011
    ..In particular, we focus on the controversial molecular mechanisms that regulate NLRP3 signaling and highlight recent advancements in DNA sensing by the inflammasome receptor AIM2...
  18. doi request reprint Immunoblotting for active caspase-1
    Christopher Jakobs
    Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Methods Mol Biol 1040:103-15. 2013
    ..Here, we present protocols for the preparation and precipitation of supernatant samples containing activated caspase-1 as well as protocols for polyacrylamide gel electrophoresis (PAGE) and protein immunoblotting. ..
  19. pmc Species-specific detection of the antiviral small-molecule compound CMA by STING
    Taner Cavlar
    Unit for Clinical Biochemistry, Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, Bonn, Germany
    EMBO J 32:1440-50. 2013
    ..Altogether, these results provide novel insight into ligand-sensing properties of STING and, furthermore, unravel unexpected species-specific differences of this innate sensor...
  20. doi request reprint iGLuc: a luciferase-based inflammasome and protease activity reporter
    Eva Bartok
    Institute for Clinical Chemistry and Clinical Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Nat Methods 10:147-54. 2013
    ..The high sensitivity, signal-to-background ratio and specificity of the iGLuc system renders it a useful tool to study proteolytic events in mouse and human cells at high throughput and to monitor protease activity in mice in vivo...
  21. ncbi request reprint An unexpected role for RNA in the recognition of DNA by the innate immune system
    Franz Bauernfeind
    Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    RNA Biol 7:151-7. 2010
    ..In this review article, we will discuss the mechanisms and implications of this novel pathway...
  22. doi request reprint Nucleic acid driven sterile inflammation
    Andrea Ablasser
    Institute for Clinical Chemistry and Clinical Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Germany
    Clin Immunol 147:207-15. 2013
    ..Indeed, the capacity of NAs to accidentally activate innate sensors significantly contributes to inappropriate responses to self. In this review we will discuss recent findings based on established disease models...
  23. pmc The NALP3 inflammasome is involved in the innate immune response to amyloid-beta
    Annett Halle
    Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Immunol 9:857-65. 2008
    ..Our findings suggest that activation of the NALP3 inflammasome is important for inflammation and tissue damage in Alzheimer's disease...
  24. pmc DNA sensing unchained
    Andrea Ablasser
    Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Cell Res 23:585-7. 2013
    ..They further unravel a key role for a so far uncharacterized murine protein E330016A19 (human homolog: C6ORF150), now termed cGAMP synthetase (cGAS), to act as the DNA sensor that generates cGAMP...
  25. doi request reprint NLRP3 inflammasome activity is negatively controlled by miR-223
    Franz Bauernfeind
    Unit for Clinical Biochemistry, Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, 53127 Bonn, Germany
    J Immunol 189:4175-81. 2012
    ..Therefore, given the tight transcriptional control of NLRP3 message itself, miR-223 functions as an important rheostat controlling NLRP3 inflammasome activity...
  26. ncbi request reprint Induction of type I IFNs by intracellular DNA-sensing pathways
    Taner Cavlar
    Institute for Clinical Chemistry and Clinical Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Immunol Cell Biol 90:474-82. 2012
    ..In this review, we outline the recent advancements in the elucidation of intracellular DNA-sensing pathways and discuss the future directions of this emerging field...
  27. ncbi request reprint Enzymatic synthesis and purification of a defined RIG-I ligand
    Marion Goldeck
    Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, Sigmund Freud Strasse 25, 53127, Bonn, Germany
    Methods Mol Biol 1169:15-25. 2014
    ..We here describe a protocol to enzymatically synthesize and to purify a defined RIG-I ligand that can be used to study RIG-I activation in vitro and in vivo. ..
  28. ncbi request reprint TREX1 deficiency triggers cell-autonomous immunity in a cGAS-dependent manner
    Andrea Ablasser
    Institute of Molecular Medicine, University Hospital, University of Bonn, 53127 Bonn, Germany and
    J Immunol 192:5993-7. 2014
    ..These findings indicate a key role of cyclic GMP-AMP synthase for the initiation of self-DNA-induced autoimmune disorders, thus providing important implications for novel therapeutic approaches. ..
  29. doi request reprint 5'-Triphosphate-siRNA: turning gene silencing and Rig-I activation against melanoma
    Hendrik Poeck
    Institute of Clinical Chemistry and Pharmacology, University Hospital, University of Bonn, Bonn, Germany
    Nat Med 14:1256-63. 2008
    ....
  30. pmc Critical functions of priming and lysosomal damage for NLRP3 activation
    Veit Hornung
    Institute for Clinical Chemistry and Pharmacology, University Hospital, University of Bonn, Bonn, Germany
    Eur J Immunol 40:620-3. 2010
    ..Here, we discuss the critical roles of priming and lysosomal damage in NLRP3 inflammasome activation...
  31. ncbi request reprint OAS proteins and cGAS: unifying concepts in sensing and responding to cytosolic nucleic acids
    Veit Hornung
    Institute of Molecular Medicine, University Hospital, University of Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany
    Nat Rev Immunol 14:521-8. 2014
    ..In this Progress article, we describe the striking functional and structural similarities between OAS proteins and cGAS, and highlight their roles in antiviral immunity. ..
  32. doi request reprint Selection of molecular structure and delivery of RNA oligonucleotides to activate TLR7 versus TLR8 and to induce high amounts of IL-12p70 in primary human monocytes
    Andrea Ablasser
    Institute of Clinical Chemistry and Pharmacology, University Hospital, University of Bonn, Bonn, Germany
    J Immunol 182:6824-33. 2009
    ....
  33. ncbi request reprint A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes
    Jonathan L Schmid-Burgk
    Institute for Clinical Chemistry and Clinical Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany
    Nat Biotechnol 31:76-81. 2013
    ..Given its simplicity, throughput and fidelity, LIC assembly will permit the generation of TAL effector gene libraries for large-scale functional genomics studies...
  34. doi request reprint Retroviral Danger from Within: TLR7 Is in Control
    Arun K Mankan
    Institute for Clinical Chemistry and Clinical Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, 53127 Bonn, Germany
    Immunity 37:763-6. 2012
    ....
  35. ncbi request reprint siRNA and isRNA: two edges of one sword
    Martin Schlee
    Division of Clinical Pharmacology, Department of Medicine, University of Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany
    Mol Ther 14:463-70. 2006
    ....