Jurgen Heesemann

Summary

Affiliation: University of Munich
Country: Germany

Publications

  1. pmc Two distinct coagulase-dependent barriers protect Staphylococcus aureus from neutrophils in a three dimensional in vitro infection model
    Christoph Guggenberger
    Max von Pettenkofer Institut, Ludwig Maximilians University of Munich LMU, Munich, Germany
    PLoS Pathog 8:e1002434. 2012
  2. pmc Tracing genomic variations in two highly virulent Yersinia enterocolitica strains with unequal ability to compete for host colonization
    Debora Garzetti
    Max von Pettenkofer Institute, LMU, Munich, Germany
    BMC Genomics 13:467. 2012
  3. pmc Contribution of trimeric autotransporter C-terminal domains of oligomeric coiled-coil adhesin (Oca) family members YadA, UspA1, EibA, and Hia to translocation of the YadA passenger domain and virulence of Yersinia enterocolitica
    Nikolaus Ackermann
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, Pettenkoferstrasse 9a, 80336 Munich, Germany
    J Bacteriol 190:5031-43. 2008
  4. pmc Approaching the secrets of N-glycosylation in Aspergillus fumigatus: characterization of the AfOch1 protein
    Andrea Kotz
    Max von Pettenkofer Institute, Ludwig Maximilians University, Munich, Germany
    PLoS ONE 5:e15729. 2010
  5. ncbi request reprint Role of strain differences on host resistance and the transcriptional response of macrophages to infection with Yersinia enterocolitica
    Katrin van Erp
    Department of Bacteriology, Max von Pettenkofer Institut, Munich, Germany
    Physiol Genomics 25:75-84. 2006
  6. ncbi request reprint Mechanisms of Yersinia enterocolitica evasion of the host innate immune response by V antigen
    Andreas Sing
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat Munchen, Pettenkoferstrasse 9a, 80336 Munchen, Germany
    Adv Exp Med Biol 529:165-7. 2003
  7. doi request reprint The mitA gene of Aspergillus fumigatus is required for mannosylation of inositol-phosphorylceramide, but is dispensable for pathogenicity
    Andrea Kotz
    Max von Pettenkofer Institute, Ludwig Maximilians University, Munich, Germany
    Fungal Genet Biol 47:169-78. 2010
  8. ncbi request reprint A dominant role of Toll-like receptor 4 in the signaling of apoptosis in bacteria-faced macrophages
    Rudolf Haase
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Munich, Germany
    J Immunol 171:4294-303. 2003
  9. doi request reprint AfMkk2 is required for cell wall integrity signaling, adhesion, and full virulence of the human pathogen Aspergillus fumigatus
    Franziska Dirr
    Max von Pettenkofer Institut, Ludwig Maximilians Universitat, Pettenkoferstraße 9a, 80336 Munich, Germany
    Int J Med Microbiol 300:496-502. 2010
  10. pmc The Yersinia enterocolitica type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1
    Svea Dittmann
    Max von Pettenkofer Institut, Lehrstuhl für Bakteriologie, Ludwig Maximilians Universitat Munchen, Munchen, Germany
    BMC Microbiol 7:67. 2007

Detail Information

Publications78

  1. pmc Two distinct coagulase-dependent barriers protect Staphylococcus aureus from neutrophils in a three dimensional in vitro infection model
    Christoph Guggenberger
    Max von Pettenkofer Institut, Ludwig Maximilians University of Munich LMU, Munich, Germany
    PLoS Pathog 8:e1002434. 2012
    ..aureus abscess formation by temporal dissection of bacterial growth and recruitment of immune cells. It can complement established animal infection models in the development of new treatment options...
  2. pmc Tracing genomic variations in two highly virulent Yersinia enterocolitica strains with unequal ability to compete for host colonization
    Debora Garzetti
    Max von Pettenkofer Institute, LMU, Munich, Germany
    BMC Genomics 13:467. 2012
    ..To trace the reasons of this incongruity, we present here the first high-quality sequence of the whole genome of strain WA-314 and compare it to the published genome of strain 8081...
  3. pmc Contribution of trimeric autotransporter C-terminal domains of oligomeric coiled-coil adhesin (Oca) family members YadA, UspA1, EibA, and Hia to translocation of the YadA passenger domain and virulence of Yersinia enterocolitica
    Nikolaus Ackermann
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, Pettenkoferstrasse 9a, 80336 Munich, Germany
    J Bacteriol 190:5031-43. 2008
    ....
  4. pmc Approaching the secrets of N-glycosylation in Aspergillus fumigatus: characterization of the AfOch1 protein
    Andrea Kotz
    Max von Pettenkofer Institute, Ludwig Maximilians University, Munich, Germany
    PLoS ONE 5:e15729. 2010
    ..Infection experiments provided no evidence for attenuation in virulence; in fact, according to our data the Δafoch1 mutant may even be slightly more virulent than the control strains...
  5. ncbi request reprint Role of strain differences on host resistance and the transcriptional response of macrophages to infection with Yersinia enterocolitica
    Katrin van Erp
    Department of Bacteriology, Max von Pettenkofer Institut, Munich, Germany
    Physiol Genomics 25:75-84. 2006
    ..These data show that host resistance factors modulate a surprisingly small, but identifiable and functionally significant, portion of the macrophage transcriptome in response to Yersinia infection...
  6. ncbi request reprint Mechanisms of Yersinia enterocolitica evasion of the host innate immune response by V antigen
    Andreas Sing
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat Munchen, Pettenkoferstrasse 9a, 80336 Munchen, Germany
    Adv Exp Med Biol 529:165-7. 2003
  7. doi request reprint The mitA gene of Aspergillus fumigatus is required for mannosylation of inositol-phosphorylceramide, but is dispensable for pathogenicity
    Andrea Kotz
    Max von Pettenkofer Institute, Ludwig Maximilians University, Munich, Germany
    Fungal Genet Biol 47:169-78. 2010
    ..fumigatus PAMPs...
  8. ncbi request reprint A dominant role of Toll-like receptor 4 in the signaling of apoptosis in bacteria-faced macrophages
    Rudolf Haase
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Munich, Germany
    J Immunol 171:4294-303. 2003
    ..Together, these data show that TLR4 could act as a potent inducer of apoptosis in macrophages that encounter a bacterial pathogen...
  9. doi request reprint AfMkk2 is required for cell wall integrity signaling, adhesion, and full virulence of the human pathogen Aspergillus fumigatus
    Franziska Dirr
    Max von Pettenkofer Institut, Ludwig Maximilians Universitat, Pettenkoferstraße 9a, 80336 Munich, Germany
    Int J Med Microbiol 300:496-502. 2010
    ..And in agreement with its sensitivity to moderate temperatures, it is less virulent in a murine infection model. Our data underline the importance of mkk2 for the fitness, but also for the pathogenicity of A. fumigatus...
  10. pmc The Yersinia enterocolitica type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1
    Svea Dittmann
    Max von Pettenkofer Institut, Lehrstuhl für Bakteriologie, Ludwig Maximilians Universitat Munchen, Munchen, Germany
    BMC Microbiol 7:67. 2007
    ..The effectors YopO and YopP are encoded on an operon together with SycO, the chaperone of YopO. The characterization of SycO is the focus of this study...
  11. ncbi request reprint Analysis of the regulation, expression, and localisation of the isocitrate lyase from Aspergillus fumigatus, a potential target for antifungal drug development
    Frank Ebel
    Max von Pettenkofer Institute, Munich, Germany
    Fungal Genet Biol 43:476-89. 2006
    ..Lipids, as potential substrates, were detected in conidia and macrophages. Additionally, germinating conidia within macrophages contain ICL, suggesting that the glyoxylate shunt might be a relevant target for development of antifungals...
  12. ncbi request reprint Analysis of chaperone-dependent Yop secretion/translocation and effector function using a mini-virulence plasmid of Yersinia enterocolitica
    Konrad Trulzsch
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, Germany
    Int J Med Microbiol 293:167-77. 2003
    ..In addition this system can secrete heterologous proteins fused to the N-terminal secretion/translocation domain of YopE...
  13. ncbi request reprint Horizontal transfer of Yersinia high-pathogenicity island by the conjugative RP4 attB target-presenting shuttle plasmid
    Uladzimir Antonenka
    Max von Pettenkofer Institut, Pettenkoferstr 9a, 80336 Munich, Germany
    Mol Microbiol 57:727-34. 2005
    ..Such 'trapping' of pathogenicity islands and subsequent shuffling to new hosts by a conjugative replicon carrying a suitable attB site could be applied to other functional integrative elements and explain wide dissemination of PAIs...
  14. ncbi request reprint Expression of Pseudomonas aeruginosa exoS is controlled by quorum sensing and RpoS
    Michael Hogardt
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians University Munich, Pettenkoferstrasse 9a, D 80336 Munich, Germany
    Microbiology 150:843-51. 2004
    ..Moreover, these data suggest that the exoenzyme S regulon may be downregulated in P. aeruginosa biofilms...
  15. pmc Unusual, virulence plasmid-dependent growth behavior of Yersinia enterocolitica in three-dimensional collagen gels
    Sandra Freund
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, D 80336 Munich, Germany
    J Bacteriol 190:4111-20. 2008
    ..Thus, this 3D-CoG model may be a first step to a more complex level of in vitro infection models that mimic living tissue, enabling us to study the dynamics of pathogen-host cell interactions...
  16. pmc Superior protective immunity against murine listeriosis by combined vaccination with CpG DNA and recombinant Salmonella enterica serovar typhimurium
    Christina Berchtold
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, 80336 Munich, Germany
    Infect Immun 77:5501-8. 2009
    ....
  17. ncbi request reprint Yersinia's stratagem: targeting innate and adaptive immune defense
    Jurgen Heesemann
    Max von Pettenkofer Institut, Pettenkoferstrasse 9 a, D 80336 Muenchen, Germany
    Curr Opin Microbiol 9:55-61. 2006
    ....
  18. ncbi request reprint Signaling of apoptosis through TLRs critically involves toll/IL-1 receptor domain-containing adapter inducing IFN-beta, but not MyD88, in bacteria-infected murine macrophages
    Klaus Ruckdeschel
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Munich, Germany
    J Immunol 173:3320-8. 2004
    ..Together, these results argue for a specific proapoptotic activity of TRIF as part of the host innate immune response to bacterial or viral infection...
  19. pmc A hypervariable N-terminal region of Yersinia LcrV determines Toll-like receptor 2-mediated IL-10 induction and mouse virulence
    Andreas Sing
    Lehrstuhl Bakteriologie, Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Pettenkoferstrasse 9a, 80336 Munich, Germany
    Proc Natl Acad Sci U S A 102:16049-54. 2005
    ..LcrV is a defined bacterial virulence factor shown to target the TLR system for evasion of the host's immune response...
  20. ncbi request reprint Molecular mechanism of YbtA-mediated transcriptional regulation of divergent overlapping promoters ybtA and irp6 of Yersinia enterocolitica
    Roman Anisimov
    Max von Pettenkofer Institute of Hygiene and Medical Microbiology Pettenkofer Strasse 9a, 80336 Munich, Germany
    FEMS Microbiol Lett 250:27-32. 2005
    ..Based on these data, we propose a model of irp6 and ybtA promoter regulation...
  21. pmc Bacterial toxins induce sustained mRNA expression of the silencing transcription factor klf2 via inactivation of RhoA and Rhophilin 1
    Kristina Dach
    Department of Bacteriology, Max von Pettenkofer Institut, Pettenkoferstr 9a, 80336 Munich, Germany
    Infect Immun 77:5583-92. 2009
    ..Thus, bacteria exploit the RhoA-rhophilin signaling cascade to mediate sustained expression of the immunosuppressive transcription factor klf2...
  22. doi request reprint Farnesol misplaces tip-localized Rho proteins and inhibits cell wall integrity signalling in Aspergillus fumigatus
    Karl Dichtl
    Max von Pettenkofer Institut, Ludwig Maximilians Universitat, Munich, Germany
    Mol Microbiol 76:1191-204. 2010
    ..After farnesol treatment this localization is rapidly lost, which is accompanied by swelling of the hyphal tips. Parallel displacement of tropomyosin from the tips suggests a concomitant disorganization of the apical actin cytoskeleton...
  23. ncbi request reprint A fundamental role of mAbp1 in neutrophils: impact on beta(2) integrin-mediated phagocytosis and adhesion in vivo
    Jurgen Schymeinsky
    Walter Brendel Centre for Experimental Medicine, Ludwig Maximilians University Munich, D 80336 Munich, Germany
    Blood 114:4209-20. 2009
    ..These unanticipated results define mAbp1 as a novel molecular player in integrin biology that is critical for phagocytosis and firm neutrophil adhesion under flow conditions...
  24. pmc The putative alpha-1,2-mannosyltransferase AfMnt1 of the opportunistic fungal pathogen Aspergillus fumigatus is required for cell wall stability and full virulence
    Johannes Wagener
    Max von Pettenkofer Institute, Ludwig Maximilians Universitat, Munich, Germany
    Eukaryot Cell 7:1661-73. 2008
    ....
  25. ncbi request reprint Staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens
    Konrad Trulzsch
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Munich, Germany
    Int J Syst Evol Microbiol 57:1543-8. 2007
    ..Due to differences in the results of physiological and chemotaxonomic investigations and DNA-DNA hybridization data, strain A6664 was not included in the description of the novel species...
  26. pmc Highly resistant metabolically deficient dwarf mutant of Escherichia coli is the cause of a chronic urinary tract infection
    Konrad Trulzsch
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Munich, Germany
    J Clin Microbiol 41:5689-94. 2003
    ..This case highlights the importance of recognizing MDDs of Enterobacteriaceae in patients with chronic infections. To our knowledge this is the first report of an MDD of E. coli causing a chronic urinary tract infection...
  27. pmc Yersinia enterocolitica palearctica serobiotype O:3/4--a successful group of emerging zoonotic pathogens
    Julia Batzilla
    Max von Pettenkofer Institute, LMU, Munich, Germany
    BMC Genomics 12:348. 2011
    ..enterocolitica ssp. enterocolitica 8081 O:8/1B to address the peculiarities of the O:3/4 group...
  28. doi request reprint Yersinia enterocolitica and Photorhabdus asymbiotica β-lactamases BlaA are exported by the twin-arginine translocation pathway
    Eva Maria Schriefer
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University of Munich, D 80336 Munich, Germany
    Int J Med Microbiol 303:16-24. 2013
    ..These results suggest that Yersinia BlaA-related β-lactamases may be the prototype of a large Tat-dependent β-lactamase family, which originated from environmental bacteria...
  29. ncbi request reprint Transcriptional responses of murine macrophages to infection with Yersinia enterocolitica
    Reinhard Hoffmann
    Max von Pettenkofer Institut, Bacteriology Department, Pettenkoferstrasse 9a, 80336 Munich, Germany
    Cell Microbiol 6:377-90. 2004
    ..We suggest that Yersinia enterocolitica might use two independent mechanisms to inhibit macrophage inflammatory responses at the transcriptional level...
  30. ncbi request reprint The proteasome pathway destabilizes Yersinia outer protein E and represses its antihost cell activities
    Klaus Ruckdeschel
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Munich, Germany
    J Immunol 176:6093-102. 2006
    ..This implies the proteasome as integral part of the cellular host immune response against the immunomodulatory activities of a translocated bacterial virulence protein...
  31. pmc Contribution of the major secreted yops of Yersinia enterocolitica O:8 to pathogenicity in the mouse infection model
    Konrad Trulzsch
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Munich, Germany
    Infect Immun 72:5227-34. 2004
    ..The DeltayopE mutant was eliminated from the small intestine 3 weeks after infection, whereas the DeltayopH, DeltayopP, DeltayopM, and DeltayopQ mutants continued to colonize the small intestine at this time...
  32. doi request reprint Characterisation of the phagocytic uptake of Aspergillus fumigatus conidia by macrophages
    Kathrin Luther
    Max von Pettenkofer Institute, Ludwig Maximilians University, Munich, Germany
    Microbes Infect 10:175-84. 2008
    ..Taken together, our data provide new insights into the internalisation of A. fumigatus spores by macrophages, a key process in the early immune defence against an Aspergillus infection...
  33. ncbi request reprint Yersinia enterocolitica inactivates NK cells
    Isabel Koch
    Ludwig Maximilians University, Max von Pettenkofer Institut, Department of Bacteriology, 80336 Munich, Germany
    Int J Med Microbiol 303:433-42. 2013
    ..Thus, we present the first report of a bacterial pathogen inactivating NK cells, and report interaction with Tyk2-STAT4 signaling as a novel function of YopP. ..
  34. pmc The two-component sensor kinase TcsC and its role in stress resistance of the human-pathogenic mold Aspergillus fumigatus
    Allison McCormick
    Max von Pettenkofer Institut, Ludwig Maximilians University, Munich, Germany
    PLoS ONE 7:e38262. 2012
    ..Although TcsC plays a role in the adaptation of A. fumigatus to hypoxia, it seems to be dispensable for virulence...
  35. ncbi request reprint Yersinia enterocolitica type III secretion chaperone SycD: recombinant expression, purification and characterization of a homodimer
    Annika Schmid
    Max von Pettenkofer Institut, Lehrstuhl für Bakteriologie, Ludwig Maximilians Universitat Munchen, Pettenkoferstr 9a, D 80336 MUnchen, Germany
    Protein Expr Purif 49:176-82. 2006
    ..To prove functionality of purified SycD previously suggested interactions of SycD with Yop secretion protein M2 (YscM2), and low calcium response protein V (LcrV), respectively, were reinvestigated...
  36. ncbi request reprint Yersinia enterocolitica YopQ: strain-dependent cytosolic accumulation and post-translational secretion
    Janja Trcek
    Max von Pettenkofer Institut, Pettenkoferstrasse 9a, D 80336 Munich, Germany
    Microbiology 148:1457-65. 2002
    ..Moreover, post-translational secretion of YopQ could be demonstrated. These findings do not support the mRNA signal hypothesis for co-translational secretion...
  37. ncbi request reprint Characterization of YopT effects on Rho GTPases in Yersinia enterocolitica-infected cells
    Martin Aepfelbacher
    Max von Pettenkofer Institut für Medizinische Mikrobiologie, Ludwig Maximilians Universitat, Pettenkoferstrasse 9a, 80336 Munich, Germany
    J Biol Chem 278:33217-23. 2003
    ..These findings indicate that bacterially translocated YopT acts on membrane-bound and GDI-complexed RhoA but not Rac or CDC42, and this is sufficient for disruption of macrophage immune functions...
  38. ncbi request reprint A novel insertion sequence element, IS Yen2, as an epidemiological marker for weakly pathogenic bioserotypes of Yersinia enterocolitica
    Andrey Golubov
    Yersinia Group I, Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Pettenkofer Str 9a, D 80336 Munich, Germany
    Int J Med Microbiol 295:213-26. 2005
    ..enterocolitica differentiation and epidemiological studies. Distribution of the different groups of IS elements in two Y. enterocolitica pathotypes is in favor of the parallel evolution of American and European Y. enterocolitica strains...
  39. pmc Transfer of the core region genes of the Yersinia enterocolitica WA-C serotype O:8 high-pathogenicity island to Y. enterocolitica MRS40, a strain with low levels of pathogenicity, confers a yersiniabactin biosynthesis phenotype and enhanced mouse virulenc
    Cosima Pelludat
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, 80336 Munich, Germany
    Infect Immun 70:1832-41. 2002
    ..Thus, the core genes of Yen-HPI are sufficient to confer a positive CAS phenotype and mouse virulence to Y. enterocolitica MRS40, BG 2, but are insufficient to confer this phenotype to Y. enterocolitica NF-O, BG 1A...
  40. ncbi request reprint Subtractive hybridization uncovers novel pathogenicity-associated loci in Yersinia enterocolitica
    Azuka N Iwobi
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Pettenkofer Street 9a, 80336 Munich, Germany
    Adv Exp Med Biol 529:25-9. 2003
  41. pmc Molecular analysis of transport and oligomerization of the Yersinia enterocolitica adhesin YadA
    Andreas Roggenkamp
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilian University, and Medical Centre Grosshadern, 81377 Munich, Germany
    J Bacteriol 185:3735-44. 2003
    ..Mutants lacking the head and neck domain were resistant to complement-mediated lysis. In-frame truncation of the stalk domain did not result in significant attenuation of the mutant in an orogastric mouse infection model...
  42. pmc Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression
    Andreas Sing
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat Munchen, Pettenkoferstrasse 9a, Germany
    J Exp Med 196:1017-24. 2002
    ..We conclude that yersiniae might exploit host innate pattern recognition molecules and defense mechanisms to evade the host immune response...
  43. pmc Yersinia enterocolitica infection of mice reveals clonal invasion and abscess formation
    Mark F Oellerich
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillians University, Pettenkoferstrasse 9a, 80336 Munich, Germany
    Infect Immun 75:3802-11. 2007
    ..Furthermore, we show that the host severely restricts sequential microcolony formation in previously infected Peyer's patches...
  44. pmc Yersinia enterocolitica type III secretion: evidence for the ability to transport proteins that are folded prior to secretion
    Gottfried Wilharm
    Max von Pettenkofer Institut, Pettenkoferstr, 9a, D 80336 MUnchen, Germany
    BMC Microbiol 4:27. 2004
    ..It was suggested that folding is prevented either by co-translational secretion or by the assistance of specific Yop chaperones (called Sycs)...
  45. ncbi request reprint Characterisation of the CipC-like protein AFUA_5G09330 of the opportunistic human pathogenic mould Aspergillus fumigatus
    Bettina Bauer
    Max von Pettenkofer Institut, Ludwig Maximilians Universitat, Munich, Germany
    Mycoses 53:296-304. 2010
    ..fumigatus during infection. Further studies are required to define the biological function of this hyphae-specific protein and its potential relevance for the pathogenicity of A. fumigatus...
  46. ncbi request reprint Uncovering genomic differences in human pathogenic Yersinia enterocolitica
    Andrey Golubov
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Pettenkofer Str 9a, 80336 Munich, Germany
    FEMS Immunol Med Microbiol 38:107-11. 2003
    ..Mapped out genomic differences may be applied in identification and development of novel therapeutic strategies for the treatment of enteropathogenic Yersinia...
  47. ncbi request reprint The Yersinia enterocolitica type 3 secretion system (T3SS) as toolbox for studying the cell biological effects of bacterial Rho GTPase modulating T3SS effector proteins
    Stefan Wolke
    Max von Pettenkofer Institut, LMU Munich, Pettenkofer Straße 9A, 80336 Munich, Germany
    Cell Microbiol 13:1339-57. 2011
    ..In summary, the Yersinia toolbox is suitable to study cellular effects of effector proteins of diverse bacterial species separately or in combination in the context of bacterial T3SS-mediated injection...
  48. ncbi request reprint Sequence variability and functional analysis of MutS of hypermutable Pseudomonas aeruginosa cystic fibrosis isolates
    Michael Hogardt
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat, Pettenkoferstrasse 9a, D 80336 MUnchen, Germany
    Int J Med Microbiol 296:313-20. 2006
    ..This work supports the hypothesis that the respiratory tract of CF patients represents an environment that favors the selection of highly adaptive mutator phenotypes...
  49. ncbi request reprint Tracing acquisitions and losses in Yersinia genomes
    Alexander Rakin
    Max von Pettenkofer Institute of Hygiene and Medical Microbiology, University of Munich, Pettenkofer Street 9a, 80336, Munich, Germany
    Adv Exp Med Biol 529:19-23. 2003
  50. ncbi request reprint Yersinia enterocolitica type III secretion chaperone SycH. Recombinant expression, purification, characterisation, and crystallisation
    Wibke Neumayer
    Max von Pettenkofer Institut, Lehrstuhl für Bakteriologie, Ludwig Maximilians Universitat Munchen, Pettenkoferstr 9a, D 80336 Munich, Germany
    Protein Expr Purif 35:237-47. 2004
    ..In addition, we found that YopH was enzymatically active in the presence of SycH. This implies that the function of the secretion chaperone SycH is not to keep YopH in a globally unfolded state prior to secretion...
  51. ncbi request reprint Yersinia as oral live carrier vaccine: influence of Yersinia outer proteins (Yops) on the T-cell response
    Konrad Trulzsch
    Max von Pettenkofer Institut, LMU Munchen, Pettenkoferstr 9a, D 80336 MUnchen, Germany
    Int J Med Microbiol 298:59-67. 2008
    ....
  52. pmc Cross-talk between type three secretion system and metabolism in Yersinia
    Annika Schmid
    Department of Bacteriology, Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Pettenkoferstrasse 9a, D 80336 Munich, Germany
    J Biol Chem 284:12165-77. 2009
    ..enterocolitica. We propose a "load-and-shoot cycle" model to account for the cross-talk between T3SS and metabolism in pathogenic yersiniae...
  53. pmc Structural organization of the pFra virulence-associated plasmid of rhamnose-positive Yersinia pestis
    Andrey Golubov
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Munich, Germany
    Infect Immun 72:5613-21. 2004
    ..pG8786 seems to be an ancient form of the pFra group of plasmids that were conserved due to the strict geographical isolation of rhamnose-positive Y. pestis strains in the high mountainous Caucasian plague locus...
  54. pmc Yersinia enterocolitica type III secretion depends on the proton motive force but not on the flagellar motor components MotA and MotB
    Gottfried Wilharm
    Bakteriologie, Max von Pettenkofer Institut, Pettenkoferstrasse 9a, D 80336 Munich, Germany
    Infect Immun 72:4004-9. 2004
    ..Thus, the hypothesis that the Ysc TTSS might adopt flagellar motor components was not confirmed. The finding that, in addition to consumption of ATP, Ysc TTSS requires the proton motive force is discussed...
  55. ncbi request reprint A novel integrative and conjugative element (ICE) of Escherichia coli: the putative progenitor of the Yersinia high-pathogenicity island
    Soren Schubert
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Lehrstuhl für Bakteriologie, Pettenkoferstr 9a, 80336 Munich, Germany
    Mol Microbiol 51:837-48. 2004
    ..It may represent the progenitor of the HPI found in Y. pestis and E. coli, revealing a missing link in the horizontal transfer of an element that contributes to microbial pathogenicity upon acquisition...
  56. ncbi request reprint Stage-specific adaptation of hypermutable Pseudomonas aeruginosa isolates during chronic pulmonary infection in patients with cystic fibrosis
    Michael Hogardt
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat, Munich, Germany
    J Infect Dis 195:70-80. 2007
    ..In cystic fibrosis (CF), such mutator strains may lead to the rapid selection of survivors that are specifically adapted to the hostile environment of the inflamed CF lung...
  57. ncbi request reprint On the role of specific chaperones, the specific ATPase, and the proton motive force in type III secretion
    Gottfried Wilharm
    Max von Pettenkofer Institut, Lehrstuhl für Bakteriologie, Ludwig Maximilians Universitat Munchen, Pettenkoferstrasse 9a, D 80336 Munich, Germany
    Int J Med Microbiol 297:27-36. 2007
    ..Here, we focus on the role of specific T3SS chaperones, the specific ATPase, and the proton motive force in type III secretion...
  58. ncbi request reprint Invasion and dissemination of Yersinia enterocolitica in the mouse infection model
    Konrad Trulzsch
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillians University, Munich, Germany
    Adv Exp Med Biol 603:279-85. 2007
    ..Only very few yersiniae invade Peyer's patches and establish just a very few monoclonal microabscesses. This is due to both Yersinia and host specific factors...
  59. pmc Molecular evidence of nosocomial Pneumocystis jirovecii transmission among 16 patients after kidney transplantation
    Sabine Schmoldt
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Marchioninstrasse 17, 81377 Munich, Germany
    J Clin Microbiol 46:966-71. 2008
    ..jirovecii as the predominant transmission route. Therefore, strict segregation and isolation of P. jirovecii-positive/suspected patients in clinical settings seems warranted...
  60. ncbi request reprint Quantification of phagocytosis of Aspergillus conidia by macrophages using a novel antibody-independent assay
    Kathrin Luther
    Max von Pettenkofer Institut, Ludwig Maximilians Universitat, Pettenkofer Strasse 9a, 80336 Munich, Germany
    J Microbiol Methods 66:170-3. 2006
    ..fumigatus infections. Here we describe a novel antibody-independent assay for quantification of phagocytosis, that in this study has been applied to different Aspergillus species, but that is in principle suitable for many fungi...
  61. ncbi request reprint Recombinant Yersinia enterocolitica YscM1 and YscM2: homodimer formation and susceptibility to thrombin cleavage
    Gottfried Wilharm
    Department of Bacteriology, Max von Pettenkofer Institut, Pettenkoferstr 9a, Munich D 80336, Germany
    Protein Expr Purif 31:167-72. 2003
    ..The YscM1 and YscM2 homodimers could be dissociated at high ionic strength, indicating that salt bridges essentially contribute to the dimerization. We further demonstrated that YscM1 and YscM2 are susceptible to thrombin cleavage...
  62. ncbi request reprint Analysis of the major proteins secreted by the human opportunistic pathogen Aspergillus fumigatus under in vitro conditions
    Monika Schwienbacher
    Max von Pettenkofer Institute, Ludwig Maximilans University, Munich, Germany
    Med Mycol 43:623-30. 2005
    ..This suggests that chitosanase, like mitogillin, is expressed during infection and might therefore be of diagnostic relevance...
  63. doi request reprint Probing the cellular effects of bacterial effector proteins with the Yersinia toolbox
    Stefan Wolke
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, LMU Munich, Pettenkofer Strasse 9a, 80336 Munich, Germany
    Future Microbiol 7:449-56. 2012
    ....
  64. pmc Attenuated Yersinia pseudotuberculosis carrier vaccine for simultaneous antigen-specific CD4 and CD8 T-cell induction
    Holger Rüssmann
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat Munchen, 80336 Munich, Germany
    Infect Immun 71:3463-72. 2003
    ..For the first time, it is shown that cytosolic antigen display mediated by an extracellular bacterial carrier vaccine results in simultaneous CD4 and CD8 T-cell priming, conferring protection against an intracellular pathogen...
  65. pmc Characterization of an iroBCDEN gene cluster on a transmissible plasmid of uropathogenic Escherichia coli: evidence for horizontal transfer of a chromosomal virulence factor
    Liisa Johanna Sorsa
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, 80336 Munich, Germany
    Infect Immun 71:3285-93. 2003
    ....
  66. ncbi request reprint Yersinia enterocolitica evasion of the host innate immune response by V antigen-induced IL-10 production of macrophages is abrogated in IL-10-deficient mice
    Andreas Sing
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Pettenkoferstrasse 9a, 80336 Munich, Germany
    J Immunol 168:1315-21. 2002
    ....
  67. pmc Detection of N-(3-oxohexanoyl)-L-homoserine lactone in mice infected with Yersinia enterocolitica serotype O8
    Christoph A Jacobi
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillian University Munich, D 80336 Munich, Germany
    Infect Immun 71:6624-6. 2003
    ..enterocolitica O8 in culture supernatant or infected mouse tissue. This is the first report demonstrating AHL production by yersiniae during infection...
  68. ncbi request reprint Yersinia outer protein P inhibits CD8 T cell priming in the mouse infection model
    Konrad Trulzsch
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat, Munchen, Germany
    J Immunol 174:4244-51. 2005
    ..Using this approach we could further show that the listeriolysin O-specific CD8 T cells generated in vivo by the yopP mutant are functional and are able to protect mice against a lethal challenge with wild type Listeria...
  69. pmc Complete genome sequence of Yersinia enterocolitica subsp. palearctica serogroup O:3
    Julia Batzilla
    Max von Pettenkofer Institute, LMU, Munich, Germany
    J Bacteriol 193:2067. 2011
    ..13030; Yersinia enterocolitica subsp. palearctica) that was isolated from the stool of a human patient (H. Neubauer, S. Aleksic, A. Hensel, E. J. Finke, and H. Meyer. Int. J. Med. Microbiol. 290:61-64, 2000)...
  70. ncbi request reprint "Staphylococcus pettenkoferi," a novel staphylococcal species isolated from clinical specimens
    Konrad Trulzsch
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Munich, Germany
    Diagn Microbiol Infect Dis 43:175-82. 2002
    ..Therefore we propose the designation of "Staphylococcus pettenkoferi" a novel species of coagulase-negative staphylococci...
  71. pmc Improvement of reporter activity by IRES-mediated polycistronic reporter system
    Hicham Bouabe
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat, 80336 Munich, Germany
    Nucleic Acids Res 36:e28. 2008
    ..Thus, endogenous gene expression and regulation, especially those controlled by weak promoters, can be analyzed with our IRES-dependent polycistronic reporter gene expression system...
  72. pmc Neisseria meningitidis adhesin NadA targets beta1 integrins: functional similarity to Yersinia invasin
    Virginie Nägele
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, 80336 Munich, Germany
    J Biol Chem 286:20536-46. 2011
    ..Thus, this study demonstrated for the first time a direct interaction between the Oca-family member NadA and human β1 integrins...
  73. pmc Yersinia high-pathogenicity island contributes to virulence in Escherichia coli causing extraintestinal infections
    Soren Schubert
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, 80336 Munich, Germany
    Infect Immun 70:5335-7. 2002
    ..coli. Moreover, using a mouse infection model, we show for the first time that the HPI contributes to the virulence of ExPEC...
  74. pmc Detection of differences in the nucleotide and amino acid sequences of diphtheria toxin from Corynebacterium diphtheriae and Corynebacterium ulcerans causing extrapharyngeal infections
    Andreas Sing
    National Consiliary Laboratory on Diphtheria, 80336 Munich, Germany
    J Clin Microbiol 41:4848-51. 2003
    ..diphtheriae DT sequences, mainly in the translocation and receptor-binding domains. C. ulcerans supernatants were much less potent than supernatant from C. diphtheriae. A C. ulcerans DT-specific PCR is described below...
  75. ncbi request reprint Pitfalls of polymyxin antimicrobial susceptibility testing of Pseudomonas aeruginosa isolated from cystic fibrosis patients
    Michael Hogardt
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, National Consulting Laboratory Southern Germany on Cystic Fibrosis Bacteriology, Ludwig Maximilians University Munich, Germany
    J Antimicrob Chemother 54:1057-61. 2004
    ....
  76. pmc The RNA chaperone Hfq impacts growth, metabolism and production of virulence factors in Yersinia enterocolitica
    Tamara Kakoschke
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Munich, Germany
    PLoS ONE 9:e86113. 2014
    ..Overall, Hfq has a profound effect on metabolism, resistance to stress and modulates the production of two virulence factors in Y. enterocolitica, namely urease and yersiniabactin. ..
  77. ncbi request reprint Reactive arthritis associated with prolonged cryptosporidial infection
    Andreas Sing
    Max von Pettenkofer Institut für Hygiene und Medizinische Mikrobiologie der LMU, Pettenkoferstrasse 9a, Munich 80336, Germany
    J Infect 47:181-4. 2003
    ..Symptoms resolved spontaneously after 20 months in parallel with stool samples negative for cryptosporidia...
  78. ncbi request reprint Prospective study on nontuberculous mycobacteria in patients with and without cystic fibrosis
    Lorenz Leitritz
    Max von Pettenkofer Institut, Ludwig Maximilians Universitat Munchen, Pettenkoferstrasse 9a, 80336 Munchen, Germany
    Med Microbiol Immunol 193:209-17. 2004
    ..Treated CF patients showed favorable clinical outcomes, as opposed to untreated patients. We propose a modified definition for diagnosis and hence treatment of NTM pulmonary infection in CF patients...