Michael Hecker

Summary

Affiliation: University of Greifswald
Country: Germany

Publications

  1. pmc A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome
    Dörte Becher
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    PLoS ONE 4:e8176. 2009
  2. pmc Bacillus subtilis: from soil bacterium to super-secreting cell factory
    Jan Maarten van Dijl
    Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, P, O, Box 30001, Groningen, 9700 RB, The Netherlands
    Microb Cell Fact 12:3. 2013
  3. pmc Integration of σB activity into the decision-making process of sporulation initiation in Bacillus subtilis
    Alexander Reder
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    J Bacteriol 194:1065-74. 2012
  4. pmc Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure
    Volkan Yildirim
    Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
    Proteome Sci 9:12. 2011
  5. pmc Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes
    Jessica C Zweers
    Department of Medical Microbiology, University Medical Center Groningen and University of Groningen, Hanzeplein 1, P, O, Box 30001, 9700 RB Groningen, The Netherlands
    Microb Cell Fact 7:10. 2008
  6. doi request reprint Gel-based proteomics of Gram-positive bacteria: a powerful tool to address physiological questions
    Michael Hecker
    Institute of Microbiology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
    Proteomics 8:4958-75. 2008
  7. ncbi request reprint Towards a comprehensive understanding of Bacillus subtilis cell physiology by physiological proteomics
    Michael Hecker
    Institute for Microbiology, Erst Moritz Arndt University, Greifswald, Germany
    Proteomics 4:3727-50. 2004
  8. ncbi request reprint Physiological proteomics and stress/starvation responses in Bacillus subtilis and Staphylococcus aureus
    Michael Hecker
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Jahnstrasse 15a, 17487 Greifswald, Germany
    Res Microbiol 160:245-58. 2009
  9. doi request reprint A proteomic view of cell physiology and virulence of Staphylococcus aureus
    Michael Hecker
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Strasse 15, D 17487 Greifswald, Germany
    Int J Med Microbiol 300:76-87. 2010
  10. ncbi request reprint SigB-dependent general stress response in Bacillus subtilis and related gram-positive bacteria
    Michael Hecker
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald 17489, Germany
    Annu Rev Microbiol 61:215-36. 2007

Detail Information

Publications126 found, 100 shown here

  1. pmc A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome
    Dörte Becher
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    PLoS ONE 4:e8176. 2009
    ..It thus paves the way towards a new level in understanding of cell physiology and pathophysiology of S. aureus and related pathogenic bacteria, opening new avenues for infection-related research on this crucial pathogen...
  2. pmc Bacillus subtilis: from soil bacterium to super-secreting cell factory
    Jan Maarten van Dijl
    Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, P, O, Box 30001, Groningen, 9700 RB, The Netherlands
    Microb Cell Fact 12:3. 2013
    ..Although highly ambitious, this is an achievable objective due to recent advances in functional genomics and Systems- and Synthetic Biological analyses of B. subtilis cells...
  3. pmc Integration of σB activity into the decision-making process of sporulation initiation in Bacillus subtilis
    Alexander Reder
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    J Bacteriol 194:1065-74. 2012
    ..subtilis...
  4. pmc Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure
    Volkan Yildirim
    Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
    Proteome Sci 9:12. 2011
    ..abstract:..
  5. pmc Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes
    Jessica C Zweers
    Department of Medical Microbiology, University Medical Center Groningen and University of Groningen, Hanzeplein 1, P, O, Box 30001, 9700 RB Groningen, The Netherlands
    Microb Cell Fact 7:10. 2008
    ..subtilis that limit the production of high quality protein complexes and membrane proteins, and to enhance those systems that facilitate assembly of these proteins...
  6. doi request reprint Gel-based proteomics of Gram-positive bacteria: a powerful tool to address physiological questions
    Michael Hecker
    Institute of Microbiology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
    Proteomics 8:4958-75. 2008
    ..However, many proteins escape detection by gel-based procedures, such as membrane or low abundance proteins. The smart combination of gel-free and gel-based approaches is the "state of the art" for physiological proteomics of bacteria...
  7. ncbi request reprint Towards a comprehensive understanding of Bacillus subtilis cell physiology by physiological proteomics
    Michael Hecker
    Institute for Microbiology, Erst Moritz Arndt University, Greifswald, Germany
    Proteomics 4:3727-50. 2004
    ....
  8. ncbi request reprint Physiological proteomics and stress/starvation responses in Bacillus subtilis and Staphylococcus aureus
    Michael Hecker
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Jahnstrasse 15a, 17487 Greifswald, Germany
    Res Microbiol 160:245-58. 2009
    ..This "road map of physiological proteomics" will be demonstrated for the general stress regulon controlled by sigma(B) in Bacillus subtilis and the oxygen starvation response with Rex as a master regulator in Staphylococcus aureus...
  9. doi request reprint A proteomic view of cell physiology and virulence of Staphylococcus aureus
    Michael Hecker
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Strasse 15, D 17487 Greifswald, Germany
    Int J Med Microbiol 300:76-87. 2010
    ..aureus can be identified and even quantified. In addition, posttranslational processes such as protein secretion, modification, processing, damages, and degradation can be adequately studied...
  10. ncbi request reprint SigB-dependent general stress response in Bacillus subtilis and related gram-positive bacteria
    Michael Hecker
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald 17489, Germany
    Annu Rev Microbiol 61:215-36. 2007
    ..A comprehensive understanding of this crucial stress response is essential not only for bacterial physiology but also for applied microbiology, including pathogenicity and pathogen control...
  11. doi request reprint Proteomic signatures uncover thiol-specific electrophile resistance mechanisms in Bacillus subtilis
    Haike Antelmann
    Institute of Microbiology, Ernst Moritz Arndt University of Greifswald, F L Jahn Str 15, D 17487 Greifswald, Germany
    Expert Rev Proteomics 5:77-90. 2008
    ..subtilis. These novel thiol-stress-responsive mechanisms are highly conserved among Gram-positive bacteria and are thought to have evolved to detoxify quinone-like electrophiles...
  12. doi request reprint Depletion of thiol-containing proteins in response to quinones in Bacillus subtilis
    Manuel Liebeke
    Institutes of Pharmaceutical Biology, Ernst Moritz Arndt University of Greifswald, F L Jahn Str 15, D 17487 Greifswald, Germany
    Mol Microbiol 69:1513-29. 2008
    ..Together our data show that the thiol-(S)-alkylation reaction with protein and non-protein thiols is the in vivo mechanism for thiol depletion and quinone toxicity in B. subtilis and most likely also in other bacteria...
  13. doi request reprint CtsR inactivation during thiol-specific stress in low GC, Gram+ bacteria
    Alexander K W Elsholz
    Institute of Microbiology, Ernst Moritz Arndt University Greifswald, F L Jahnstr 15, D 17487 Greifswald, Germany
    Mol Microbiol 79:772-85. 2011
    ..Moreover, we show that in those low GC, Gram+ bacteria, which lack the McsA/McsB complex, the Zn finger protein ClpE is able to sense and respond to oxidative stress, also resulting in CtsR inactivation...
  14. ncbi request reprint The phosphate-starvation response of Bacillus licheniformis
    Le Thi Hoi
    Institut fur Pharmazie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Proteomics 6:3582-601. 2006
    ..Data of this study indicate that, unlike in B. subtilis, phosphate starvation in B. licheniformis does not induce the SigmaB-dependent general stress response...
  15. ncbi request reprint The sigmaB regulon in Staphylococcus aureus and its regulation
    Jan Pane-Farre
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Str 15, D 17487 Greifswald, Germany
    Int J Med Microbiol 296:237-58. 2006
    ..These analyses confirmed our DNA microarray data and furthermore revealed different regulatory groups of sigmaB-dependent genes...
  16. pmc Life and death of proteins: a case study of glucose-starved Staphylococcus aureus
    Stephan Michalik
    Institute of Microbiology, Ernst Moritz Arndt University, Greifswald, Germany
    Mol Cell Proteomics 11:558-70. 2012
    ....
  17. doi request reprint The influence of SaeRS and σ(B) on the expression of superantigens in different Staphylococcus aureus isolates
    Kathrin Kusch
    Institut fur Mikrobiologie, Universitat Greifswald, Greifswald, Germany
    Int J Med Microbiol 301:488-99. 2011
    ..For sigB mutants in different strains, different effects on the T cell-mitogenic potential were observed depending on the SAg gene repertoire of the isolates...
  18. ncbi request reprint Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR)
    Thi Thu Huyen Nguyen
    Institute for Microbiology, Ernst Moritz Arndt University of Greifswald, D 17487 Greifswald, Germany
    Mol Microbiol 71:876-94. 2009
    ..We speculate that AdhR is redox-regulated via thiol-(S)-alkylation by aldehydes and that AdhA and YraA are specifically involved in reduction of aldehydes and degradation or repair of damaged thiol-containing proteins respectively...
  19. ncbi request reprint A comprehensive proteome map of growing Bacillus subtilis cells
    Christine Eymann
    Institute for Microbiology, Ernst Moritz Arndt University, Greifswald, Germany
    Proteomics 4:2849-76. 2004
    ..The vegetative proteome containing 876 proteins in total is now ready for physiological applications. Two main proteome fractions (pI 4-7 and zoom gel pI 4.5-5.5) should be sufficient for such high-throughput physiological proteomics...
  20. ncbi request reprint Proteome analyses of Staphylococcus aureus in growing and non-growing cells: a physiological approach
    Christian Kohler
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Str 15, 17487 Greifswald, Germany
    Int J Med Microbiol 295:547-65. 2005
    ..In contrast, enzymes of the TCC and gluconeogenesis are increased at the stationary phase. With this comprehensive proteome map we have an essential tool for a better understanding of cell physiology of the human pathogen, S. aureus...
  21. ncbi request reprint The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis
    Stefanie Töwe
    Institute for Microbiology, Ernst Moritz Arndt University of Greifswald, F L Jahn Str 15, D 17487 Greifswald, Germany
    Mol Microbiol 66:40-54. 2007
    ..Moreover, the DeltaykvE mutant displayed a 2-MHQ and catechol resistant phenotype. YkvE was renamed as MhqR controlling a 2-MHQ and catechol-resistance regulon of B. subtilis...
  22. ncbi request reprint Proteome signatures for stress and starvation in Bacillus subtilis as revealed by a 2-D gel image color coding approach
    Le Thi Tam
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    Proteomics 6:4565-85. 2006
    ....
  23. pmc The paralogous MarR/DUF24-family repressors YodB and CatR control expression of the catechol dioxygenase CatE in Bacillus subtilis
    Bui Khanh Chi
    Institute for Microbiology, Ernst Moritz Arndt University of Greifswald, F L Jahn Str 15, D 17487 Greifswald, Germany
    J Bacteriol 192:4571-81. 2010
    ..Redox regulation of CatR occurs independently of YodB, and no protein interaction was detected between CatR and YodB in vivo using protein cross-linking and mass spectrometry...
  24. pmc CtsR, the Gram-positive master regulator of protein quality control, feels the heat
    Alexander K W Elsholz
    Institute of Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    EMBO J 29:3621-9. 2010
    ....
  25. doi request reprint The peroxide stress response of Bacillus licheniformis
    Rebecca Schroeter
    Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, Friedrich Ludwig Jahn Strasse 17, Greifswald, Germany
    Proteomics 11:2851-66. 2011
    ..Metabolomic analyses support the induction of the glyoxylate cycle during oxidative stress in B. licheniformis...
  26. pmc Anaerobic gene expression in Staphylococcus aureus
    Stephan Fuchs
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Str 15, D 17487 Greifswald, Germany
    J Bacteriol 189:4275-89. 2007
    ..g., SACOL0201, SACOL2360, and SACOL2658) were found to be upregulated during anaerobic growth, indicating a role in the regulation of anaerobic gene expression...
  27. doi request reprint The redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis
    Bui Khanh Chi
    Institute for Microbiology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
    Proteomics 10:3155-64. 2010
    ..This study reveals a thiol-disulfide switch model of redox-regulation for the YodB repressor to sense electrophilic compounds in vivo...
  28. pmc A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus
    Christian Kohler
    Institut fur Mikrobiologie, Universitat Greifswald, Greifswald, Germany
    J Bacteriol 190:6351-64. 2008
    ..g., PflB, Ald1, IlvA1) or in the menD mutant (arc operon). Only genes involved in nitrate respiration and the ald1 operon seem to be additionally regulated by a depletion of oxygen in the hemB and/or menD mutant...
  29. pmc A comprehensive proteomics and transcriptomics analysis of Bacillus subtilis salt stress adaptation
    Hannes Hahne
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, Friedrich Ludwig Jahn Str 15, D 17489 Greifswald, Germany
    J Bacteriol 192:870-82. 2010
    ..In total, osmotic upregulation of about 500 B. subtilis genes was detected. Our data provide an unprecedented rich basis for further in-depth investigation of the physiological and genetic responses of B. subtilis to hyperosmotic stress...
  30. doi request reprint The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon
    Birgit Voigt
    Institute for Microbiology, University of Greifswald, Greifswald, Germany
    Proteomics 13:2140-61. 2013
    ..subtilis, such as BLi03885, encoding a hypothetical protein, the Na/solute symporter gene BLi02212, the arginase homolog-encoding gene BLi00198 and mcrA, encoding a protein with endonuclease activity. ..
  31. doi request reprint Distribution and infection-related functions of bacillithiol in Staphylococcus aureus
    Dierk Christoph Pöther
    Institute of Microbiology, Ernst Moritz Arndt University of Greifswald, 17487 Greifswald, Germany
    Int J Med Microbiol 303:114-23. 2013
    ..In addition, a higher bacterial load of the BSH-producing strain was detected in human upper-airway epithelial cells and murine macrophages. This indicates a potential role of BSH in protection of S. aureus during infection...
  32. ncbi request reprint The extracellular proteome of Bacillus licheniformis grown in different media and under different nutrient starvation conditions
    Birgit Voigt
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Friedrich Ludwig Jahn Strasse 15, 17487 Greifswald, Germany
    Proteomics 6:268-81. 2006
    ..Limitation of phosphate, carbon and nitrogen sources results in the secretion of specific proteins that may be involved in counteracting the starvation...
  33. pmc Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis
    Alexander K W Elsholz
    Institute of Microbiology, Ernst Moritz Arndt University Greifswald, D 17487 Greifswald, Germany
    Proc Natl Acad Sci U S A 109:7451-6. 2012
    ....
  34. pmc Diamide triggers mainly S Thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus aureus
    Dierk Christoph Pöther
    Institute of Microbiology1 and Institute of Pharmaceutical Biology, 2 Ernst Moritz Arndt University of Greifswald, F L Jahnstr 15, D 17487 Greifswald, Germany
    J Bacteriol 191:7520-30. 2009
    ..Finally, we found that a glutathione-producing B. subtilis strain which expresses the Listeria monocytogenes gshF gene did not show enhanced oxidative stress resistance compared to the wild type...
  35. doi request reprint Proteomic analysis of antioxidant strategies of Staphylococcus aureus: diverse responses to different oxidants
    Carmen Wolf
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Proteomics 8:3139-53. 2008
    ....
  36. doi request reprint Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis
    Birgit Voigt
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    J Mol Microbiol Biotechnol 16:53-68. 2009
    ..licheniformis have been analyzed under different growth conditions allowing comparisons of the extracellular proteomes and conclusions regarding similarities and differences of the protein secretion mechanisms between the two species...
  37. pmc Aureolib - a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology
    Stephan Fuchs
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    PLoS ONE 8:e70669. 2013
    ..In summary, Aureolib is by far the most comprehensive protein expression database for S. aureus and provides an essential tool to decipher more complex adaptation processes in S. aureus during host pathogen interaction. ..
  38. doi request reprint Monitoring of changes in the membrane proteome during stationary phase adaptation of Bacillus subtilis using in vivo labeling techniques
    Annette Dreisbach
    Interfakultäres Institut für Genetik und Funktionelle Genomforschung, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Proteomics 8:2062-76. 2008
    ..Both, SILAC as well as (14)N/(15)N-labeling are compatible with 2-DE, 2-D LC-MS/MS, and GeLC-MS/MS and thus will allow comprehensive simultaneous interrogation of cytoplasmic and enriched membrane proteomes...
  39. pmc Global analysis of the Staphylococcus aureus response to mupirocin
    Swantje Reiss
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Antimicrob Agents Chemother 56:787-804. 2012
    ..e., arlRS, saeRS, sarA, sarR, sarS, and sigB), as well as genes directly involved in the virulence of S. aureus (i.e., fnbA, epiE, epiG, and seb)...
  40. doi request reprint Distinctive patterns in the human antibody response to Staphylococcus aureus bacteremia in carriers and non-carriers
    Julia Kolata
    Institute of Immunology and Transfusion Medicine, University of Greifswald, Fleischmannstrasse 42 44, Greifswald, Germany
    Proteomics 11:3914-27. 2011
    ..Nevertheless, a common signature of 11 conserved S. aureus proteins, recognized in at least half of the bacteremic patients, was identified. All patients mounted a dynamic antibody response to a subset of these proteins...
  41. ncbi request reprint Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches
    Susanne Wolff
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, Friedrich Ludwig Jahn Str 15, D 17487 Greifswald, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 849:129-40. 2007
    ..subtilis theoretical proteome...
  42. pmc Nitric oxide stress induces different responses but mediates comparable protein thiol protection in Bacillus subtilis and Staphylococcus aureus
    Falko Hochgräfe
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    J Bacteriol 190:4997-5008. 2008
    ..Obviously, NO itself does not cause general protein thiol oxidations. In contrast, exposure of cells to NO prior to peroxide stress diminished the irreversible thiol oxidation caused by hydrogen peroxide...
  43. doi request reprint The Spx paralogue MgsR (YqgZ) controls a subregulon within the general stress response of Bacillus subtilis
    Alexander Reder
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, D 17487 Greifswald, Germany
    Mol Microbiol 69:1104-20. 2008
    ..Due to the strict sigma(B)-dependent expression of YqgZ it was renamed to MgsR (modulator of the general stress response)...
  44. ncbi request reprint Fluorescence thiol modification assay: oxidatively modified proteins in Bacillus subtilis
    Falko Hochgräfe
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, F L Jahn Strasse 15, D 17487 Greifswald, Germany
    Mol Microbiol 58:409-25. 2005
    ..Finally, evidence is presented that the antibiotic nitrofurantoin mediates the formation of oxidized thiols in B. subtilis...
  45. ncbi request reprint Regulation of acetoin and 2,3-butanediol utilization in Bacillus licheniformis
    Trung Nguyen Thanh
    Institut fur Pharmazie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Appl Microbiol Biotechnol 87:2227-35. 2010
    ..It is demonstrated that the acoABCL operon is mainly responsible for acetoin and 2,3-butanediol degradation in B. licheniformis...
  46. doi request reprint Proteolysis during long-term glucose starvation in Staphylococcus aureus COL
    Stephan Michalik
    Institute of Microbiology, Ernst Moritz Arndt University, D 17487 Greifswald, Germany
    Proteomics 9:4468-77. 2009
    ..Proteolysis of putative non-substrate-bound or "unemployed" proteins appears to be a characteristic feature of S. aureus in order to access nutrients as an important survival strategy under starvation conditions...
  47. doi request reprint Protecs, a comprehensive and powerful storage and analysis system for OMICS data, applied for profiling the anaerobiosis response of Staphylococcus aureus COL
    Stephan Fuchs
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Proteomics 10:2982-3000. 2010
    ..Interestingly, especially genes with a high codon adaptation index highly overlap with anaerobically induced genes...
  48. ncbi request reprint Immune cell activation by enterotoxin gene cluster (egc)-encoded and non-egc superantigens from Staphylococcus aureus
    Dorothee Grumann
    Institute for Immunology and Transfusion Medicine, University of Greifswald, Greifswald, Germany
    J Immunol 181:5054-61. 2008
    ..We conclude that the distinct biological behavior of egc and non-egc superantigens is not due to their intrinsic properties, which are very similar, but caused by their differential release by S. aureus...
  49. pmc Role of RsbU in controlling SigB activity in Staphylococcus aureus following alkaline stress
    Jan Pane-Farre
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Str 15, D 17487 Greifswald, Germany
    J Bacteriol 191:2561-73. 2009
    ..aureus RsbU. Collectively, the data suggest that the activity of the S. aureus RsbU and hence SigB may be subjected to different regulation in comparison to that in B. subtilis...
  50. doi request reprint A metabolomics and proteomics study of the adaptation of Staphylococcus aureus to glucose starvation
    Manuel Liebeke
    Institute of Pharmacy, Ernst Moritz Arndt University Greifswald, F L Jahn Str 17, D 17487 Greifswald, Germany
    Mol Biosyst 7:1241-53. 2011
    ..These alterations in levels of metabolic enzymes were paralleled by more pronounced changes in the concentrations of associated metabolites, in particular, intermediates of the glycolysis and several amino acids...
  51. ncbi request reprint The glucose and nitrogen starvation response of Bacillus licheniformis
    Birgit Voigt
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Proteomics 7:413-23. 2007
    ..Among them were yvyD, citA and the putative methylcitrate shunt genes mmgD, mmgE and yqiQ. However, both starvation conditions did not induce a general SigmaB-dependent stress response...
  52. doi request reprint Common versus noble Bacillus subtilis differentially responds to air and argon gas plasma
    Theresa Winter
    Institute for Microbiology, Ernst Moritz Arndt University, Greifswald, Germany
    Proteomics 13:2608-21. 2013
    ..Biological findings were supported by the detection of reactive plasma species by optical emission spectroscopy and Fourier transformed infrared spectroscopy measurements. ..
  53. doi request reprint Improved sandwich-hybridization assay for an electrical DNA-chip-based monitoring of bioprocess-relevant marker genes
    Daniel Pioch
    Pharmazeutische Biotechnologie, Institut fur Pharmazie, Ernst Moritz Arndt Universitat, Friedrich Ludwig Jahn Strasse 17, 17487 Greifswald, Germany
    Appl Microbiol Biotechnol 78:719-28. 2008
    ..The expression profiles determined with the electrical chip and the optimized protocol were, in most cases, comparable with the profiles determined by real-time RT-PCR measurements...
  54. pmc Global characterization of disulfide stress in Bacillus subtilis
    Lars Ingo Ole Leichert
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, 17487 Greifswald, Germany
    J Bacteriol 185:1967-75. 2003
    ....
  55. ncbi request reprint Oxidative stress triggers thiol oxidation in the glyceraldehyde-3-phosphate dehydrogenase of Staphylococcus aureus
    Harald Weber
    Institut fur Mikrobiologie, Universitat Greifswald, Jahnstrasse 15, 17487 Greifswald, Germany
    Mol Microbiol 52:133-40. 2004
    ..Our data indicate that the damaged Gap protein was not repaired...
  56. doi request reprint Cell surface proteome of the marine planctomycete Rhodopirellula baltica
    Birgit Voigt
    Institute for Microbiology, Ernst Moritz Arndt University, Greifswald, Germany
    Proteomics 12:1781-91. 2012
    ..baltica, were detected in the surface proteome. Additionally, 8 proteins with a novel protein structure combining a conserved type IV pilin/N-methylation domain and a planctomycete-typical DUF1559 domain were identified...
  57. pmc Bacillus subtilis during feast and famine: visualization of the overall regulation of protein synthesis during glucose starvation by proteome analysis
    Jörg Bernhardt
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, 17487 Greifswald, Germany
    Genome Res 13:224-37. 2003
    ....
  58. pmc Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis
    Alexander Reder
    Ernst Moritz Arndt Universität Institut für Mikrobiologie, Greifswald, Germany
    J Bacteriol 194:3601-10. 2012
    ....
  59. ncbi request reprint Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis
    Le Thi Tam
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    J Mol Microbiol Biotechnol 12:121-30. 2007
    ..Many genes encoding proteins of so far unknown functions could be assigned to specifically or commonly induced genes...
  60. doi request reprint Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach
    Kristina Hempel
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    J Proteome Res 9:1579-90. 2010
    ..Our approach revealed 28 surface-associated proteins not previously reported as SigB-dependent or -influenced, expanding the group of surface-associated proteins and virulence factors modulated by SigB...
  61. ncbi request reprint Proteomic survey through secretome of Bacillus subtilis
    Haike Antelmann
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    Methods Biochem Anal 49:179-208. 2006
  62. pmc Transcriptional organization and posttranscriptional regulation of the Bacillus subtilis branched-chain amino acid biosynthesis genes
    Ulrike Mäder
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universitat Greifswald, D 17487 Greifswald, Germany
    J Bacteriol 186:2240-52. 2004
    ..Therefore, differential segmental stability resulting from mRNA processing ensures the fine-tuning of the expression of the individual genes of the operon...
  63. ncbi request reprint Salt stress adaptation of Bacillus subtilis: a physiological proteomics approach
    Dirk Höper
    Institute for Microbiology, Ernst Moritz Arndt University, Greifswald, Germany
    Proteomics 6:1550-62. 2006
    ..This proteomic approach provides an overview of cell adaptation to an osmotic upshift in B. subtilis visualizing the most dramatic changes in the protein synthesis pattern...
  64. doi request reprint Electrical protein array chips for the detection of staphylococcal virulence factors
    Annett Quiel
    Pharmazeutische Biotechnologie, Institut fur Pharmazie, Ernst Moritz Arndt Universitat, 17487 Greifswald, Germany
    Appl Microbiol Biotechnol 85:1619-27. 2010
    ..Furthermore, a simultaneous detection of SEB of S. aureus and IsaA homologue of S. epidermidis in a single assay could be demonstrated...
  65. ncbi request reprint Transcriptome and proteome analysis of Bacillus subtilis gene expression in response to superoxide and peroxide stress
    Jörg Mostertz
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universitat Greifswald, D 17487 Greifswald, Germany
    Microbiology 150:497-512. 2004
    ..Several genes encoding proteins of unknown function could be assigned to one of these groups...
  66. ncbi request reprint The influence of agr and sigmaB in growth phase dependent regulation of virulence factors in Staphylococcus aureus
    Anne Kathrin Ziebandt
    Institut fur Mikrobiologie, Universitat Greifswald, Greifswald, Germany
    Proteomics 4:3034-47. 2004
    ..The protein pattern of the RsbU+ strain (RN6390 RsbU+) was very similar to that of the Deltaagr/DeltarsbU mutant strain (RN6911) indicating an opposing effect of agr and rsbU on the expression of the same genes...
  67. pmc Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis
    Dirk Höper
    Institute for Microbiology, Ernst Moritz Arndt University of Greifswald, Friedrich Ludwig Jahn Str 15, D 17487 Greifswald, Germany
    J Bacteriol 187:2810-26. 2005
    ..Moreover, the data imply that a secondary oxidative stress seems to be a common component of the severe stresses tested...
  68. ncbi request reprint An acetoin-regulated expression system of Bacillus subtilis
    Jörg Silbersack
    Department of Pharmaceutical Biotechnology, Institute of Pharmacy, Ernst Moritz Arndt University, 17487 Greifswald, Germany
    Appl Microbiol Biotechnol 73:895-903. 2006
    ..During glucose limitation, the utilization of the alternative energy source acetoin keeps the protein synthesis machinery of B. subtilis cells active and thus allows for a long lasting acoA-controlled expression of recombinant genes...
  69. ncbi request reprint Application of an electric DNA-chip for the expression analysis of bioprocess-relevant marker genes of Bacillus subtilis
    Britta Jürgen
    Institute of Microbiology, Ernst Moritz Arndt University Greifswald, F L Jahnstrasse 15, D 17487 Greifswald, Germany
    Biotechnol Bioeng 92:299-307. 2005
    ..We demonstrate that the mRNA analysis by means of the electric DNA-chip gives similar results compared to the micro array analysis and the real time RT-PCR technique...
  70. pmc Human immune proteome in experimental colonization with Staphylococcus aureus
    Silva Holtfreter
    Institute of Immunology and Transfusion Medicine, University of Greifswald, Greifswald, Germany
    Clin Vaccine Immunol 16:1607-14. 2009
    ..Thus, the high antibody prevalence in humans is likely not induced by short-term nasal colonization, and presumably minor infections are required to trigger anti-S. aureus antibody responses...
  71. doi request reprint Proteomics uncovers extreme heterogeneity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation
    Anne Kathrin Ziebandt
    Institute for Microbiology, University of Greifswald, Greifswald, Germany
    Proteomics 10:1634-44. 2010
    ..Importantly, the extensive variability of secreted virulence factors in S. aureus also suggests that development of protective vaccines against this pathogen requires a carefully selected combination of invariably produced antigens...
  72. doi request reprint From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches
    Hannes Hahne
    Institute for Microbiology, Ernst Moritz Arndt University, Greifswald, Germany
    Proteomics 8:4123-36. 2008
    ..subtilis cells, thus representing approximately 69% of all transcribed IMPs...
  73. pmc Involvement of Bacillus subtilis ClpE in CtsR degradation and protein quality control
    Marcus Miethke
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Str 15, D 17487 Greifswald, Germany
    J Bacteriol 188:4610-9. 2006
    ..These observations indicate the involvement of ClpE in global protein disaggregation. As a characteristic structural element of ClpE, the N-terminal zinc finger domain was proven to be essential for basal in vitro ATPase activity...
  74. pmc Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
    Martin Pagels
    Institute for Microbiology, University of Greifswald, Greifswald, Germany
    Mol Microbiol 76:1142-61. 2010
    ..aureus. Since the activity of lactate dehydrogenase enables S. aureus to resist NO stress and thus the innate immune response, our data suggest that deactivation of Rex is a prerequisite for this phenomenon...
  75. pmc Systems-wide temporal proteomic profiling in glucose-starved Bacillus subtilis
    Andreas Otto
    Ernst Moritz Arndt Universitat Greifswald, Institute for Microbiology, Greifswald 17487, Germany
    Nat Commun 1:137. 2010
    ..The obtained data comprise the first comprehensive profiling of changes in the membrane subfraction and allow in-depth analysis of major physiological processes, including monitoring of protein degradation...
  76. ncbi request reprint Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways
    Hans Matti Blencke
    Lehrstuhl fur Mikrobiologie, Institut fur Mikrobiologie, Biochemie und Genetik der Friedrich Alexander Universität Erlangen Nürnberg, Staudtstr 5, D 91058 Erlangen, Germany
    Metab Eng 5:133-49. 2003
    ..The genes encoding enzymes of the pentose phosphate pathway are expressed both in the presence and the absence of glucose, as suggested by the central role of this pathway in generating anabolic precursors...
  77. ncbi request reprint Global expression profiling of Bacillus subtilis cells during industrial-close fed-batch fermentations with different nitrogen sources
    Britta Jürgen
    Institute of Microbiology, Ernst Moritz Arndt University Greifswald, F L Jahnstrasse 15, D 17487 Greifswald, Germany
    Biotechnol Bioeng 92:277-98. 2005
    ..subtilis fermentation processes. The data suggest for example acoA as a marker gene for glucose limitation or glnA as an indicator for nitrogen limitation...
  78. doi request reprint The role of thioredoxin TrxA in Bacillus subtilis: a proteomics and transcriptomics approach
    Jörg Mostertz
    Institute of Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Proteomics 8:2676-90. 2008
    ..Thus, its function in sulfate assimilation renders TrxA an essential protein in growing B. subtilis cells...
  79. pmc Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis
    Ulf Gerth
    Ernst Moritz Arndt University, Institute of Microbiology, F L Jahn Str 15, D 17487 Greifswald, Germany
    J Bacteriol 190:321-31. 2008
    ..Proteins that are obviously nonfunctional, unprotected, or even "unemployed" seem to be recognized and proteolyzed by Clp proteases when the resources for growth become limited...
  80. ncbi request reprint S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress
    Falko Hochgräfe
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, D 17487 Greifswald, Germany
    J Biol Chem 282:25981-5. 2007
    ..By means of multidimensional shotgun proteomics, the sites of S-cysteinylation for six proteins could be identified, three of which are known to be S-glutathionylated in other organisms...
  81. ncbi request reprint Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis
    Christine Eymann
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Proteomics 7:3509-26. 2007
    ..For RsbRA, B, and D, phosphorylation of one of the conserved threonine residues in their C-termini were verified by MS (T171, T186, T181, respectively)...
  82. ncbi request reprint Monitoring of stress responses
    Thomas Schweder
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Jahnstrasse 15, 17487 Greifswald, Germany
    Adv Biochem Eng Biotechnol 89:47-71. 2004
    ..This chapter gives an overview of current RNA analysis techniques. The bacterial stress and starvation responses, which are of potential importance for industrial microbial bioprocesses are summarised...
  83. doi request reprint Status quo in physiological proteomics of the uncultured Riftia pachyptila endosymbiont
    Stephanie Markert
    Institute of Marine Biotechnology, Greifswald, Germany
    Proteomics 11:3106-17. 2011
    ..Integrating this information into complex pathway models a comprehensive survey of the symbiotic physiology was established...
  84. doi request reprint Changing the phospholipid composition of Staphylococcus aureus causes distinct changes in membrane proteome and membrane-sensory regulators
    Susanne Sievers
    Institute for Microbiology, Department of Microbial Physiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Proteomics 10:1685-93. 2010
    ..These data indicate very critical interactions of membrane-sensory proteins with phospholipids and they demonstrate the power of membrane proteomics for the characterization of bacterial physiology and pathogenicity...
  85. pmc Binding of sigma(A) and sigma(B) to core RNA polymerase after environmental stress in Bacillus subtilis
    Claudia Rollenhagen
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, 17487 Greifswald, Germany
    J Bacteriol 185:35-40. 2003
    ..Thus, the concentration of sigma(B) after stress is not high enough to compensate for its relatively low affinity for E, and it seems that additional mechanisms must be invoked to account for the binding of sigma(B) to E after stress...
  86. pmc Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis
    Christine Eymann
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, D 17487 Greifswald, Germany
    J Bacteriol 184:2500-20. 2002
    ..A passive model is preferred to explain this positive control relying on the redistribution of the RNA polymerase under the influence of (p)ppGpp...
  87. ncbi request reprint Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis
    Van Duy Nguyen
    Institute for Microbiology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
    Proteomics 7:1391-408. 2007
    ..The ycnDE operon was most strongly induced by chromanon. Mutational analyses revealed that the putative hydroquinone-specific dioxygenases MhqO and MhqA confer resistance to 2-MHQ in B. subtilis...
  88. pmc The ClpP peptidase is the major determinant of bulk protein turnover in Bacillus subtilis
    Holger Kock
    Ernst Moritz Arndt Universitat, Institut fur Mikrobiologie, F L Jahn Str 15, 17487 Greifswald, Germany
    J Bacteriol 186:5856-64. 2004
    ....
  89. ncbi request reprint A proteomic view of cell physiology of Bacillus licheniformis
    Birgit Voigt
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Proteomics 4:1465-90. 2004
    ..The data indicate that proteomics can be a valuable tool to describe the physiological state of B. licheniformis cell populations, e.g., of cells growing in a bioreactor...
  90. pmc Bacillus subtilis tolerance of moderate concentrations of rifampin involves the sigma(B)-dependent general and multiple stress response
    Julia Elisabeth Bandow
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, 17489 Greifswald, Germany
    J Bacteriol 184:459-67. 2002
    ..We conclude that the general stress response of B. subtilis is induced by rifampin depending on RsbP activity and that loss of SigB function causes increased sensitivity to the antibiotic...
  91. ncbi request reprint Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis
    Le Thi Tam
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, F L Jahn Strasse 15, D 17487 Greifswald, Germany
    Environ Microbiol 8:1408-27. 2006
    ..subtilis in the presence of catechol. Thus, our studies revealed that the catechol-2,3-dioxygenase YfiE is the key enzyme of a meta cleavage pathway in B. subtilis involved in the catabolism of catechol...
  92. pmc In vivo phosphorylation patterns of key stressosome proteins define a second feedback loop that limits activation of Bacillus subtilis σB
    Christine Eymann
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, F L Jahn Str 15, D 17487 Greifswald, Germany
    Mol Microbiol 80:798-810. 2011
    ..Loss of RsbX function increases the fraction of phosphorylated RsbS and doubly phosphorylated RsbRA in unstressed cells. We propose that RsbX both maintains the ready state of the stressosome prior to stress and restores it post-stress...
  93. doi request reprint From the genome sequence to the protein inventory of Bacillus subtilis
    Dörte Becher
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Proteomics 11:2971-80. 2011
    ..Currently, it is possible to identify and quantify more than 50% of the predicted proteome in different cellular subfractions. In this review, we summarize the development of B. subtilis proteomics during the past 25 years...
  94. ncbi request reprint Proteomics of Staphylococcus aureus--current state and future challenges
    Michael Hecker
    Institut für Mikrobiologie Jahnstrasse 15, D 17487 Greifswald, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 787:179-95. 2003
    ....
  95. ncbi request reprint The extracellular and cytoplasmic proteomes of the non-virulent Bacillus anthracis strain UM23C1-2
    Haike Antelmann
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    Proteomics 5:3684-95. 2005
    ..In addition, both of these B. anthracis compartment-specific proteomes can be used as reference maps to monitor changes in the production of secreted and cytosolic proteins that occur, for example, during growth in macrophages...
  96. pmc Fine-tuning in regulation of Clp protein content in Bacillus subtilis
    Ulf Gerth
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, D 17487 Greifswald, Germany
    J Bacteriol 186:179-91. 2004
    ..Analysis of the half-life of stress-inducible clp mRNAs during exponential growth and heat shock revealed precise regulation of the synthesis of each Clp protein at the posttranscriptional level as well to meet the needs of B. subtilis...
  97. ncbi request reprint A proteomic view of cell physiology of Bacillus subtilis--bringing the genome sequence to life
    Michael Hecker
    Ernst Moritz Arndt Universitat Greifswald, Institut fur Mikrobiologie, F L Jahn Strasse 15, 17487 Greifswald, Germany
    Adv Biochem Eng Biotechnol 83:57-92. 2003
    ....
  98. ncbi request reprint MurAA, catalysing the first committed step in peptidoglycan biosynthesis, is a target of Clp-dependent proteolysis in Bacillus subtilis
    Holger Kock
    Ernst Moritz Arndt Universitat Greifswald, Institut fur Mikrobiologie und Molekularbiologie, Germany
    Mol Microbiol 51:1087-102. 2004
    ..Its function as a regulatory metabolic checkpoint resembles that of homoserine trans-succinylase (MetA) in E. coli which is similarly ATP-dependently degraded...
  99. ncbi request reprint Quantitative proteome profiling during the fermentation process of pleiotropic Bacillus subtilis mutants
    Haike Antelmann
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    Proteomics 4:2408-24. 2004
    ..Instead, the overproduction of degradative enzymes in both of these mutants was reflected in the extracellular proteome...
  100. pmc Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability
    Ulrike Mäder
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, D 17487 Greifswald, Germany
    J Bacteriol 184:4288-95. 2002
    ..Determination of sporulation frequencies confirmed the physiological relevance of the expression data...
  101. ncbi request reprint Stabilization of cell wall proteins in Bacillus subtilis: a proteomic approach
    Haike Antelmann
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    Proteomics 2:591-602. 2002
    ..We conclude that WapA processing products are substrates for the extracellular proteases which are stabilized in the absence of sigD due to an impaired cell wall turnover...