Affiliation: University Hospital
Hannig C, Spitzmüller B, Knausenberger S, Hoth Hannig W, Hellwig E, Hannig M. Detection and activity of peroxidase in the in situ formed enamel pellicle. Arch Oral Biol. 2008;53:849-58 pubmed publisher
..Peroxidase activity is present in the pellicle already after 3 min of formation time, but is inhibited by the substrate and polyphenolic beverages. ..
Hannig C, Spitzmüller B, Hannig M. Transaminases in the acquired pellicle. Arch Oral Biol. 2009;54:445-8 pubmed publisher
..52 mU/cm(2)). The ALT-activity of the centrifuged saliva was 26.62+/-11.09 mU/ml (AST 35.98+/-29.35 mU/ml). AST as well as ALT are present in the in situ pellicle layer and may contribute to the intrinsic maturation of pellicle proteins. ..
Hannig C, Hannig M, Rehmer O, Braun G, Hellwig E, Al Ahmad A. Fluorescence microscopic visualization and quantification of initial bacterial colonization on enamel in situ. Arch Oral Biol. 2007;52:1048-56 pubmed
..Already after 3 min, adherent bacteria are present in the initial pellicle. For the first time, DAPI-staining as well as FISH have proven success for visualization of initial intraoral colonization of enamel specimens. ..
Hannig C, Hannig M. The oral cavity--a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin Oral Investig. 2009;13:123-39 pubmed publisher
..For evaluation of biofilm formation on dental materials, in situ or in vivo studies are preferable. ..
Hannig C, Spies B, Spitzmüller B, Hannig M. Efficacy of enzymatic mouth rinses for immobilisation of protective enzymes in the in situ pellicle. Arch Oral Biol. 2010;55:1-6 pubmed publisher
..With the tested enzymatic mouth rinses targeted accumulation and immobilisation of protective enzymes in the in situ pellicle did not seem possible. ..
Hannig C, Sorg J, Spitzmüller B, Hannig M, Al Ahmad A. Polyphenolic beverages reduce initial bacterial adherence to enamel in situ. J Dent. 2009;37:560-6 pubmed publisher
..Also FISH revealed significant impact of most tested beverages. Rinses with certain polyphenolic beverages as well as consumption of these foodstuffs may contribute to prevention of biofilm induced diseases in the oral cavity. ..
Hannig C, Duong S, Becker K, Brunner E, Kahler E, Attin T. Effect of bleaching on subsurface micro-hardness of composite and a polyacid modified composite. Dent Mater. 2007;23:198-203 pubmed
..To investigate the influence of different bleaching techniques on subsurface physical properties of composite and polyacid modified composite tested via determination of micro-hardness...
Hannig C, Huber K, Lambrichts I, Gräser J, D Haen J, Hannig M. Detection of salivary alpha-amylase and lysozyme exposed on the pellicle formed in situ on different materials. J Biomed Mater Res A. 2007;83:98-103 pubmed
..Both enzymes are distributed randomly on the surface of the pellicle, irrespective of the underlying substratum...
Hannig C, Spitzmüller B, Miller M, Hellwig E, Hannig M. Intrinsic enzymatic crosslinking and maturation of the in situ pellicle. Arch Oral Biol. 2008;53:416-22 pubmed publisher
..It has been supposed that the pellicle undergoes maturation after protein adsorption. The aim of the present study was to investigate enzyme activities with an impact on intrinsic maturation processes in in situ formed pellicles...