R Hakenbeck

Summary

Affiliation: University of Kaiserslautern
Country: Germany

Publications

  1. pmc The genome of Streptococcus mitis B6--what is a commensal?
    Dalia Denapaite
    Department of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany
    PLoS ONE 5:e9426. 2010
  2. pmc A new variant of the capsule 3 cluster occurs in Streptococcus pneumoniae from deceased wild chimpanzees
    Dalia Denapaite
    Department of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany
    PLoS ONE 6:e25119. 2011
  3. pmc Cell surface proteins in S. pneumoniae, S. mitis and S. oralis
    A Madhour
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Str 23, D 67663 Kaiserslautern
    Iran J Microbiol 3:58-67. 2011
  4. pmc Altered lipid composition in Streptococcus pneumoniae cpoA mutants
    Marina Meiers
    Department of Microbiology, University of Kaiserslautern, Gottlieb Daimler Strasse, Gebäude 23, D 67663 Kaiserslautern, Germany
    BMC Microbiol 14:12. 2014
  5. pmc Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
    Kelly L Wyres
    Department of Zoology, University of Oxford, Oxford, United Kingdom
    BMC Genomics 14:500. 2013
  6. doi request reprint The capsule of Streptococcus pneumoniae contributes to virulence in the insect model Manduca sexta
    Angelika Roth
    Department of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany
    J Mol Microbiol Biotechnol 22:326-34. 2012
  7. doi request reprint Molecular mechanisms of β-lactam resistance in Streptococcus pneumoniae
    Regine Hakenbeck
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    Future Microbiol 7:395-410. 2012
  8. pmc Identification of genes for small non-coding RNAs that belong to the regulon of the two-component regulatory system CiaRH in Streptococcus
    Patrick Marx
    Department of Microbiology, University of Kaiserslautern, D 67663 Kaiserslautern, Germany
    BMC Genomics 11:661. 2010
  9. pmc The CiaRH system of Streptococcus pneumoniae prevents lysis during stress induced by treatment with cell wall inhibitors and by mutations in pbp2x involved in beta-lactam resistance
    Thorsten Mascher
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    J Bacteriol 188:1959-68. 2006
  10. pmc Mosaic genes and mosaic chromosomes: intra- and interspecies genomic variation of Streptococcus pneumoniae
    R Hakenbeck
    Universitat Kaiserslautern, D 67663 Kaiserslautern, Germany
    Infect Immun 69:2477-86. 2001

Collaborators

Detail Information

Publications47

  1. pmc The genome of Streptococcus mitis B6--what is a commensal?
    Dalia Denapaite
    Department of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany
    PLoS ONE 5:e9426. 2010
    ..mitis as well and confirm their importance for the pathogenetic potential of S. pneumoniae. Despite the close relatedness between the two species, the S. mitis B6 genome reveals a striking X-alignment when compared with S. pneumoniae...
  2. pmc A new variant of the capsule 3 cluster occurs in Streptococcus pneumoniae from deceased wild chimpanzees
    Dalia Denapaite
    Department of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany
    PLoS ONE 6:e25119. 2011
    ..In summary, the data indicate that cps3(Taï) evolved independent from other cps3 loci, suggesting the presence of specialized serotype 3 S. pneumoniae clones endemic to the Taï National Park area...
  3. pmc Cell surface proteins in S. pneumoniae, S. mitis and S. oralis
    A Madhour
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Str 23, D 67663 Kaiserslautern
    Iran J Microbiol 3:58-67. 2011
    ..mitis B6 in silico. We tested for the presence of such genes usinga set of eight S. mitis and eleven S. oralis strains from different geographic locations...
  4. pmc Altered lipid composition in Streptococcus pneumoniae cpoA mutants
    Marina Meiers
    Department of Microbiology, University of Kaiserslautern, Gottlieb Daimler Strasse, Gebäude 23, D 67663 Kaiserslautern, Germany
    BMC Microbiol 14:12. 2014
    ..The CpoA gene is part of an operon including another putative glycosyltransferase gene spr0982, both of which being homologous to glycolipid synthases present in other Gram-positive bacteria...
  5. pmc Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
    Kelly L Wyres
    Department of Zoology, University of Oxford, Oxford, United Kingdom
    BMC Genomics 14:500. 2013
    ..In this study the genomes of 38 pneumococci isolated prior to 1974 were probed for the presence of tet(M), cat, erm(B), mef(A/E) and int (integrase) to indicate the presence of Tn916/Tn5252-like elements...
  6. doi request reprint The capsule of Streptococcus pneumoniae contributes to virulence in the insect model Manduca sexta
    Angelika Roth
    Department of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany
    J Mol Microbiol Biotechnol 22:326-34. 2012
    ..S. pneumoniae R6 transformants producing the type 6B capsule showed a similar elevated disease potential, confirming the contribution of the pneumococcal polysaccharide capsule to virulence in M. sexta...
  7. doi request reprint Molecular mechanisms of β-lactam resistance in Streptococcus pneumoniae
    Regine Hakenbeck
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    Future Microbiol 7:395-410. 2012
    ..Depending on the selective β-lactam, different combinations of PBP genes and mutations within are involved in conferring resistance, and astoundingly in non-PBP genes as well...
  8. pmc Identification of genes for small non-coding RNAs that belong to the regulon of the two-component regulatory system CiaRH in Streptococcus
    Patrick Marx
    Department of Microbiology, University of Kaiserslautern, D 67663 Kaiserslautern, Germany
    BMC Genomics 11:661. 2010
    ..Knowledge on the participation of csRNAs in CiaRH-dependent regulatory events will be the key to define the physiological role of this important control system...
  9. pmc The CiaRH system of Streptococcus pneumoniae prevents lysis during stress induced by treatment with cell wall inhibitors and by mutations in pbp2x involved in beta-lactam resistance
    Thorsten Mascher
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    J Bacteriol 188:1959-68. 2006
    ....
  10. pmc Mosaic genes and mosaic chromosomes: intra- and interspecies genomic variation of Streptococcus pneumoniae
    R Hakenbeck
    Universitat Kaiserslautern, D 67663 Kaiserslautern, Germany
    Infect Immun 69:2477-86. 2001
    ....
  11. ncbi request reprint Transformation in Streptococcus pneumoniae: mosaic genes and the regulation of competence
    R Hakenbeck
    University of Kaiserslautern, Department of Microbiology, Germany
    Res Microbiol 151:453-6. 2000
    ..Expression of comC is part of a complex regulatory network involving at least two receptor kinase/transcriptional regulator pairs: ComD/E, which is responsible for induction, and CiaH/R, which inhibits expression of the comCDE operon...
  12. pmc Acquisition of five high-Mr penicillin-binding protein variants during transfer of high-level beta-lactam resistance from Streptococcus mitis to Streptococcus pneumoniae
    R Hakenbeck
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    J Bacteriol 180:1831-40. 1998
    ..The growth rates of all transformants were identical to that of the parental S. pneumoniae strain. The results stress the potential for the acquisition by S. pneumoniae of high-level beta-lactam resistance by interspecies gene transfer...
  13. ncbi request reprint Mosaic genes and their role in penicillin-resistant Streptococcus pneumoniae
    R Hakenbeck
    Universitat Kaiserslautern, Germany
    Electrophoresis 19:597-601. 1998
    ....
  14. ncbi request reprint Resistant penicillin-binding proteins
    R Hakenbeck
    Institut fur Mikrobiologie, Universitat Kaiserslautern, Germany
    Cell Mol Life Sci 54:332-40. 1998
    ..Enterococci have a natural low susceptibility to beta-lactams related to the presence of an intrinsic low-affinity PBP. Highly resistant enterococcal strains overexpress this PBP and/or reduce its affinity...
  15. ncbi request reprint beta-lactam resistance in Streptococcus pneumoniae: penicillin-binding proteins and non-penicillin-binding proteins
    R Hakenbeck
    University of Kaiserslautern, Department of Microbiology, Kaiserslautern, Germany
    Mol Microbiol 33:673-8. 1999
    ....
  16. ncbi request reprint Versatility of choline metabolism and choline-binding proteins in Streptococcus pneumoniae and commensal streptococci
    Regine Hakenbeck
    Department of Microbiology, University of Kaiserslautern, Germany
    FEMS Microbiol Rev 33:572-86. 2009
    ..mitis. In contrast, the virulence-associated CBPs, CbpA, PspA and PcpA, are S. pneumoniae specific and are thus relevant for the characteristic properties of this species...
  17. ncbi request reprint Penicillin-binding proteins as resistance determinants in clinical isolates of Streptococcus pneumoniae
    P Reichmann
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Microb Drug Resist 2:177-81. 1996
    ..In contrast, high-level cefotaxime resistance appeared to be due to a low affinity PBP2a, indicating that this PBP can also function as a resistance determinant...
  18. pmc A novel resistance mechanism against beta-lactams in Streptococcus pneumoniae involves CpoA, a putative glycosyltransferase
    T Grebe
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    J Bacteriol 179:3342-9. 1997
    ..This suggests that the cpo operon has a similar function related to cell surface components...
  19. ncbi request reprint Mosaic pbpX genes of major clones of penicillin-resistant Streptococcus pneumoniae have evolved from pbpX genes of a penicillin-sensitive Streptococcus oralis
    C Sibold
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Mol Microbiol 12:1013-23. 1994
    ..These data are in agreement with the assumption that PBPs as penicillin-resistance determinants have evolved by the accumulation of point mutations in genes of sensitive commensal species...
  20. pmc Nucleotide sequences of genes encoding penicillin-binding proteins from Streptococcus pneumoniae and Streptococcus oralis with high homology to Escherichia coli penicillin-binding proteins 1a and 1b
    C Martin
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    J Bacteriol 174:4517-23. 1992
    ..pneumoniae PBP 1a. Both PBPs are highly homologous to bifunctional high-M(r) Escherichia coli PBPs 1a and 1b...
  21. ncbi request reprint Nucleotide sequences of the pbpX genes encoding the penicillin-binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime-resistant mutant, C506
    G Laible
    Max Planck Institut fur Molekulare Genetik, Berlin, FRG
    Mol Microbiol 3:1337-48. 1989
    ..coli PBP2 and methicillin-resistant Staphylococcus aureus PBP2'. No significant homology was detected with PBP1a or PBP1b of Escherichia coli, or with the low-Mr PBPs...
  22. ncbi request reprint The fib locus in Streptococcus pneumoniae is required for peptidoglycan crosslinking and PBP-mediated beta-lactam resistance
    B Weber
    Univerität Kaiserslautern, Abteilung Mikrobiologie, Paul Erlich Strasse, Kaiserslautern, Germany
    FEMS Microbiol Lett 188:81-5. 2000
    ..FibA and FibB belong to a family of proteins of Gram-positive bacteria involved in the formation of interpeptide bridges, thus representing interesting new targets for antimicrobial compounds for this group of pathogens...
  23. ncbi request reprint Penicillin-binding proteins in Streptococcus pneumoniae: alterations during development of intrinsic penicillin resistance
    R Hakenbeck
    , Berlin, Germany
    J Chemother 3:86-90. 1991
    ..The variability of the three PBPs analyzed implies a very heterogeneous gene pool accessible to the pneumococcus that is used for recruitment of resistant PBP genes in wild type strains...
  24. ncbi request reprint Interspecies recombinational events during the evolution of altered PBP 2x genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae
    G Laible
    Max Planck Institut für Molekelare Genetik, Berlin, Germany
    Mol Microbiol 5:1993-2002. 1991
    ....
  25. ncbi request reprint A two-component signal-transducing system is involved in competence and penicillin susceptibility in laboratory mutants of Streptococcus pneumoniae
    E Guenzi
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Mol Microbiol 12:505-15. 1994
    ....
  26. pmc Relatedness of penicillin-binding protein 1a genes from different clones of penicillin-resistant Streptococcus pneumoniae isolated in South Africa and Spain
    C Martin
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    EMBO J 11:3831-6. 1992
    ..We suggest that the type 23F clone originated in Spain prior to distribution into other continents...
  27. pmc A mutation in the D,D-carboxypeptidase penicillin-binding protein 3 of Streptococcus pneumoniae contributes to cefotaxime resistance of the laboratory mutant C604
    J Krauss
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Antimicrob Agents Chemother 41:936-42. 1997
    ..None of the pbp3 genes had a mosaic structure, but in three cases there was evidence for a site-specific recombination event within a BOX element immediately downstream of pbp3...
  28. ncbi request reprint Allelic variation in a peptide-inducible two-component system of Streptococcus pneumoniae
    P Reichmann
    Department of Microbiology, University of Kaiserslautern, Germany
    FEMS Microbiol Lett 190:231-6. 2000
    ..A direct repeat element upstream of the spiR1 promoter and another three potential transcription start sites within the bacteriocin cluster indicate that SpiP functions as an inducing peptide for bacteriocin synthesis in S. pneumoniae...
  29. ncbi request reprint Novel plasmids in clinical strains of Streptococcus pneumoniae
    C Sibold
    Max Planck Institut fur Molekulare Genetik, Berlin, F R G
    FEMS Microbiol Lett 61:91-5. 1991
    ..6 kb and 2.7 kb, respectively. These two plasmids hybridized neither with each other, nor with pDP1, demonstrating that they represent new types of plasmids not having been found in pneumococci before...
  30. ncbi request reprint Identification of the genes directly controlled by the response regulator CiaR in Streptococcus pneumoniae: five out of 15 promoters drive expression of small non-coding RNAs
    Alexander Halfmann
    Department of Microbiology, University of Kaiserslautern, D 67663 Kaiserslautern, Germany
    Mol Microbiol 66:110-26. 2007
    ..The identification of five small non-coding regulatory RNAs opens new perspectives to approach the physiological role of the CiaRH two-component regulatory system...
  31. ncbi request reprint Crossing the barrier: evolution and spread of a major class of mosaic pbp2x in Streptococcus pneumoniae, S. mitis and S. oralis
    Fang Chi
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Str 23, D 67663 Kaiserslautern, Germany
    Int J Med Microbiol 297:503-12. 2007
    ..mitis strains from South Africa and Spain, facilitating the identification of pbp2x mutations relevant for resistance development...
  32. pmc The Streptococcus pneumoniae beta-galactosidase is a surface protein
    D Zahner
    Department of Microbiology, University of Kaiserslautern, D 67663 Kaiserslautern, Germany
    J Bacteriol 182:5919-21. 2000
    ..The results are consistent with beta-galactosidase being a surface protein that seems not to be involved in lactose metabolism but that may play a role during pathogenesis...
  33. ncbi request reprint The ciaR/ciaH regulatory network of Streptococcus pneumoniae
    Dorothea Zahner
    University of Kaiserslautern, Department of Microbiology, Germany
    J Mol Microbiol Biotechnol 4:211-6. 2002
    ..The DNA binding property of CiaR and ComE were exploited to isolate specifically interacting DNA fragments as a first step to identify genes targeted by individual response regulators...
  34. doi request reprint Penicillin-binding protein 2x of Streptococcus pneumoniae: three new mutational pathways for remodelling an essential enzyme into a resistance determinant
    Patrick Maurer
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    J Mol Biol 376:1403-16. 2008
    ..In each group, at least one mutation is located in close vicinity to the active site and mediates a resistance phenotype in vivo alone, whereas other mutations might exhibit secondary effects only in context with other alterations...
  35. pmc An important site in PBP2x of penicillin-resistant clinical isolates of Streptococcus pneumoniae: mutational analysis of Thr338
    Ilka Zerfass
    Department of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany
    Antimicrob Agents Chemother 53:1107-15. 2009
    ..The data support the view that PBP2x and PBP1a interact with each other on some level and that alterations of both PBPs in resistant clinical isolates have evolved to ensure cooperation between both proteins...
  36. pmc The Streptococcus pneumoniae cia regulon: CiaR target sites and transcription profile analysis
    Thorsten Mascher
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    J Bacteriol 185:60-70. 2003
    ..Furthermore, the competence regulon, including the comCDE operon required for induction of competence, was completely repressed by the cia system...
  37. pmc A functional dlt operon, encoding proteins required for incorporation of d-alanine in teichoic acids in gram-positive bacteria, confers resistance to cationic antimicrobial peptides in Streptococcus pneumoniae
    Martá Kovács
    University of Kaiserslautern, Department of Microbiology, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    J Bacteriol 188:5797-805. 2006
    ..pneumoniae contain d-alanine residues in order to protect this human pathogen against the actions of cationic antimicrobial peptides...
  38. ncbi request reprint A new integrative reporter plasmid for Streptococcus pneumoniae
    Alexander Halfmann
    Department of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany
    FEMS Microbiol Lett 268:217-24. 2007
    ..The new integrative plasmid offers several advantages compared with existing systems and is especially suited for stable integration of small promoter fragments to conduct mutagenesis or deletion studies...
  39. pmc The two-component regulatory system TCS08 is involved in cellobiose metabolism of Streptococcus pneumoniae R6
    Stuart J McKessar
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    J Bacteriol 189:1342-50. 2007
    ..pneumoniae and homologues were found in Streptococcus gordonii only, suggesting that this system might contribute to the pathogenicity potential of the pneumococcus...
  40. pmc Genome of Streptococcus oralis strain Uo5
    Peter Reichmann
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Str 23, D 67663 Kaiserslautern, Germany
    J Bacteriol 193:2888-9. 2011
    ..Comparative and functional genomics of Uo5 will be important in understanding the evolution of pathogenesis among Mitis streptococci and their potential to engage in interspecies gene transfer...
  41. pmc New Streptococcus pneumoniae clones in deceased wild chimpanzees
    Fang Chi
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, D 67663 Kaiserslautern, Germany
    J Bacteriol 189:6085-8. 2007
    ..The findings suggest that virulent S. pneumoniae occurs in populations of wild chimpanzees with the potential to cause infections similar to those observed in humans...
  42. pmc Diversity of bacteriocins and activity spectrum in Streptococcus pneumoniae
    Thomas Lux
    Department of Microbiology, Nano Bio Center, University of Kaiserslautern, Erwin Schrödinger Str 13, Postfach 3049, Kaiserslautern, Germany
    J Bacteriol 189:7741-51. 2007
    ..At least one other bacteriocin gene, pncE, is located elsewhere on the chromosome and might contribute to the bacteriocin activity of this strain...
  43. ncbi request reprint Mosaic genes and mosaic chromosomes-genomic variation in Streptococcus pneumoniae
    Reinhold Brückner
    Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Strasse 23, 67663 Kaiserslautern, Germany
    Int J Med Microbiol 294:157-68. 2004
    ....
  44. ncbi request reprint A XerD recombinase with unusual active site motifs in Streptococcus pneumoniae
    Peter Reichmann
    Department of Microbiology, University of Kaiserslautern, Germany
    J Mol Microbiol Biotechnol 4:101-10. 2002
    ..The xerD and xerC genes were present in a sample of 20 S. pneumoniae strains whereas the other xer genes appear to be absent in some of the strains and are more closely related to integrases of phage and transposon origin...
  45. pmc Highly variable penicillin resistance determinants PBP 2x, PBP 2b, and PBP 1a in isolates of two Streptococcus pneumoniae clonal groups, Poland 23F-16 and Poland 6B-20
    Radoslaw Izdebski
    National Medicines Institute, Division of Clinical Microbiology and Infection Prevention, Chełmska 30 34, Warsaw 00 725, Poland
    Antimicrob Agents Chemother 52:1021-7. 2008
    ..The data document a highly variable pool of PBP genes as a result of multiple gene transfer and recombination events within and between different clonal groups...
  46. ncbi request reprint Characterization of German penicillin non-susceptible serotype 23F pneumococci using multilocus sequence typing
    Ralf Rene Reinert
    Institute of Medical Microbiology, National Reference Center for Streptococci, University Hospital, D 52057 Aachen, Germany
    J Med Microbiol 52:981-7. 2003
    ..4 %) have sequence types that have not been reported to date from other countries (STs 353-362). The major penicillin-resistant clones are present in Germany, a country with relatively low levels of beta-lactam resistance...
  47. pmc Different pathways of choline metabolism in two choline-independent strains of Streptococcus pneumoniae and their impact on virulence
    Arun S Kharat
    Laboratory of Microbiology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    J Bacteriol 190:5907-14. 2008
    ....