Stephan Gunther

Summary

Affiliation: University of Hamburg
Country: Germany

Publications

  1. pmc Hospital-based surveillance for viral hemorrhagic fevers and hepatitides in Ghana
    Joseph Humphrey Kofi Bonney
    Virology Department, Noguchi Memorial Institute of Medical Research, University of Ghana, Legon, Ghana Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS Negl Trop Dis 7:e2435. 2013
  2. pmc Lassa fever, Nigeria, 2003 and 2004
    Sunday Aremu Omilabu
    Emerg Infect Dis 11:1642-4. 2005
  3. pmc Novel arenavirus sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Côte d'Ivoire: implications for evolution of arenaviruses in Africa
    David Coulibaly-N'golo
    Laboratoire des arbovirus entérovirus, Institut Pasteur de Côte d Ivoire, Abidjan, Cote d Ivoire
    PLoS ONE 6:e20893. 2011
  4. pmc Lassa Fever, Nigeria, 2005-2008
    Deborah U Ehichioya
    University of Lagos, Lagos, Nigeria D U Ehichioya, S A Omilabu Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany M Hass, S Olschlager, B Becker Ziaja, S Gunther Ebonyi State University Teaching Hospital, Abakaliki, Nigeria C O Onyebuchi Chukwu Federal Ministry of Health, Abuja, Nigeria J Coker, A Nasidi and National Hospital, Abuja O O Ogogbu
    Emerg Infect Dis 16:1040-1. 2010
  5. pmc Functional dissection of the alphavirus capsid protease: sequence requirements for activity
    Saijo Thomas
    Department of Vectorology and Experimental Gene Therapy, Biomedical Research Center, University of Rostock, D 18057 Rostock, Germany
    Virol J 7:327. 2010
  6. ncbi Lassa fever encephalopathy: Lassa virus in cerebrospinal fluid but not in serum
    S Gunther
    Bernhard Nocht Institut fur Tropenmedizin, D 20359 Hamburg, Germany
    J Infect Dis 184:345-9. 2001
  7. pmc Mopeia virus-related arenavirus in natal multimammate mice, Morogoro, Tanzania
    Stephan Gunther
    Bernhard Nocht Institute for Tropical Medicine Department of Virology, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    Emerg Infect Dis 15:2008-12. 2009
  8. ncbi Genetic variation in HBV infection: genotypes and mutants
    Stephan Gunther
    Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany
    J Clin Virol 36:S3-S11. 2006
  9. ncbi Lassa virus
    Stephan Gunther
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany
    Crit Rev Clin Lab Sci 41:339-90. 2004
  10. ncbi Antibodies to Lassa virus Z protein and nucleoprotein co-occur in human sera from Lassa fever endemic regions
    S Gunther
    Bernhard Nocht Institut fur Tropenmedizin, Hamburg, Germany
    Med Microbiol Immunol 189:225-9. 2001

Collaborators

Detail Information

Publications64

  1. pmc Hospital-based surveillance for viral hemorrhagic fevers and hepatitides in Ghana
    Joseph Humphrey Kofi Bonney
    Virology Department, Noguchi Memorial Institute of Medical Research, University of Ghana, Legon, Ghana Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS Negl Trop Dis 7:e2435. 2013
    ..This study was conducted to determine if VHF and viral hepatitis contribute to hospital morbidity in the Central and Northern parts of Ghana...
  2. pmc Lassa fever, Nigeria, 2003 and 2004
    Sunday Aremu Omilabu
    Emerg Infect Dis 11:1642-4. 2005
  3. pmc Novel arenavirus sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Côte d'Ivoire: implications for evolution of arenaviruses in Africa
    David Coulibaly-N'golo
    Laboratoire des arbovirus entérovirus, Institut Pasteur de Côte d Ivoire, Abidjan, Cote d Ivoire
    PLoS ONE 6:e20893. 2011
    ..Reconstruction of possible virus-host co-phylogeny scenarios suggests that, within the African continent, signatures of co-evolution might have been obliterated by multiple host-switching events...
  4. pmc Lassa Fever, Nigeria, 2005-2008
    Deborah U Ehichioya
    University of Lagos, Lagos, Nigeria D U Ehichioya, S A Omilabu Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany M Hass, S Olschlager, B Becker Ziaja, S Gunther Ebonyi State University Teaching Hospital, Abakaliki, Nigeria C O Onyebuchi Chukwu Federal Ministry of Health, Abuja, Nigeria J Coker, A Nasidi and National Hospital, Abuja O O Ogogbu
    Emerg Infect Dis 16:1040-1. 2010
    ..The current epidemiologic situation is less clear because no surveillance system is in place...
  5. pmc Functional dissection of the alphavirus capsid protease: sequence requirements for activity
    Saijo Thomas
    Department of Vectorology and Experimental Gene Therapy, Biomedical Research Center, University of Rostock, D 18057 Rostock, Germany
    Virol J 7:327. 2010
    ..All alphaviruses analyzed to date show this autocatalytic cleavage. Here we have analyzed the sequence requirements for the cleavage activity of Chikungunya virus capsid protease of genus alphavirus...
  6. ncbi Lassa fever encephalopathy: Lassa virus in cerebrospinal fluid but not in serum
    S Gunther
    Bernhard Nocht Institut fur Tropenmedizin, D 20359 Hamburg, Germany
    J Infect Dis 184:345-9. 2001
    ..Lassa virus infection should be considered in West African patients or in travelers returning from this area who present only with fever and neurologic signs...
  7. pmc Mopeia virus-related arenavirus in natal multimammate mice, Morogoro, Tanzania
    Stephan Gunther
    Bernhard Nocht Institute for Tropical Medicine Department of Virology, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    Emerg Infect Dis 15:2008-12. 2009
    ..Only a small percentage of mice carry Morogoro virus, although a large proportion shows specific antibodies...
  8. ncbi Genetic variation in HBV infection: genotypes and mutants
    Stephan Gunther
    Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany
    J Clin Virol 36:S3-S11. 2006
  9. ncbi Lassa virus
    Stephan Gunther
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany
    Crit Rev Clin Lab Sci 41:339-90. 2004
    ..Several aspects ranging from basic research up to clinical practice and laboratory diagnosis are discussed and linked together...
  10. ncbi Antibodies to Lassa virus Z protein and nucleoprotein co-occur in human sera from Lassa fever endemic regions
    S Gunther
    Bernhard Nocht Institut fur Tropenmedizin, Hamburg, Germany
    Med Microbiol Immunol 189:225-9. 2001
    ..001). These data provide evidence for appearance of antibodies to Z protein and NP following Lassa virus infection. A recombinant blot for detection of both antibody specificities seems to be specific but less sensitive than IIF...
  11. doi Management of accidental exposure to Ebola virus in the biosafety level 4 laboratory, Hamburg, Germany
    Stephan Gunther
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Infect Dis 204:S785-90. 2011
    ..Otherwise, the person remained healthy, and ZEBOV RNA, except for the glycoprotein gene expressed in the vaccine, was never detected in serum and peripheral blood mononuclear cells during the 3-week observation period...
  12. ncbi Enhanced replication contributes to enrichment of hepatitis B virus with a deletion in the core gene
    S Gunther
    Bernhard Nocht Institut fur Tropenmedizin, Hamburg, 20359, Federal Republic of Germany
    Virology 273:286-99. 2000
    ..If the variants constitute a small part of the ccc DNA, they can be fully trans-complemented by wild-type virus which may increase the overall virus production...
  13. pmc An N-terminal region of Lassa virus L protein plays a critical role in transcription but not replication of the virus genome
    Michaela Lelke
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
    J Virol 84:1934-44. 2010
    ..Bioinformatics and cell biological experiments lend support to the hypothesis that this region could be part of a cap-snatching enzyme...
  14. doi Novel approach to differentiate subclades of varicella-zoster virus genotypes E1 and E2 in Germany
    Jonas Schmidt-Chanasit
    Bernhard Nocht Institute for Tropical Medicine, Department of Virology, D 20359 Hamburg, Germany
    Virus Res 145:347-9. 2009
    ..The classification of E1 and E2 subclades may facilitate a more exact and in-depth monitoring of the molecular evolution of VZV in Germany in the future...
  15. pmc Improved detection of Lassa virus by reverse transcription-PCR targeting the 5' region of S RNA
    Stephan Olschlager
    Bernhard Nocht Institute for Tropical Medicine, Department of Virology, Bernhard Nocht Strasse 74, Hamburg 20359, Germany
    J Clin Microbiol 48:2009-13. 2010
    ..in 1994, for the detection of Lassa virus is described. Compared to the assay developed in 1994, the GPC RT-PCR/2007 assay offers improved sensitivity for the detection of Liberian and Nigerian Lassa virus strains...
  16. pmc Cross-species analysis of the replication complex of Old World arenaviruses reveals two nucleoprotein sites involved in L protein function
    Romy Kerber
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    J Virol 85:12518-28. 2011
    ..Residue 53 and the C-terminal domain of NP are important for function of L protein during genome replication and transcription...
  17. ncbi A 12-year molecular survey of clinical herpes simplex virus type 2 isolates demonstrates the circulation of clade A and B strains in Germany
    Jonas Schmidt-Chanasit
    Bernhard Nocht Institute for Tropical Medicine, Department of Virology, D 20359 Hamburg, Germany
    J Clin Virol 48:208-11. 2010
    ..Recently two different herpes simplex virus type 2 (HSV-2) clades (A and B) were described on DNA sequence data of the glycoprotein E (gE), G (gG) and I (gI) genes...
  18. pmc Replicon system for Lassa virus
    Meike Hass
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Virol 78:13793-803. 2004
    ..It is a tool for investigating the mechanisms of replication and transcription of Lassa virus and may facilitate the testing of antivirals outside a biosafety level 4 laboratory...
  19. ncbi RT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene
    Simon Vieth
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    Trans R Soc Trop Med Hyg 101:1253-64. 2007
    ....
  20. ncbi Reverse ELISA for IgG and IgM antibodies to detect Lassa virus infections in Africa
    Petra Emmerich
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, D 20356 Hamburg, Germany
    J Clin Virol 37:277-81. 2006
    ..Anti-Lassa antibodies are detected by indirect immunofluorescence assay (IFA) or by enzyme-immunoassay (ELISA). Both methods have problems to detect low amounts of specific antibodies...
  21. pmc Current molecular epidemiology of Lassa virus in Nigeria
    Deborah U Ehichioya
    Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria
    J Clin Microbiol 49:1157-61. 2011
    ..The Bayesian analysis also provided estimates for divergence times within the Lassa virus clade...
  22. pmc Hantavirus disease outbreak in Germany: limitations of routine serological diagnostics and clustering of virus sequences of human and rodent origin
    Stefan Schilling
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 45:3008-14. 2007
    ..These findings link the outbreak with a novel PUUV lineage, "Bavaria," circulating in the local rodent population. The Bavaria lineage associated with the outbreak is only distantly related to other PUUV lineages from Germany...
  23. ncbi Prevalence of hepatitis B virus DNA in anti-HBc-positive/HBsAg-negative sera correlates with HCV but not HIV serostatus
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Clin Virol 29:59-68. 2004
    ..Hepatitis B virus (HBV) DNA often remains detectable in serum despite clinical recovery and loss of HBsAg...
  24. pmc Inhibition of different Lassa virus strains by alpha and gamma interferons and comparison with a less pathogenic arenavirus
    Marcel Asper
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, 20359 Hamburg, Germany
    J Virol 78:3162-9. 2004
    ..Both cytokines inhibit replication which is relevant for the design of antiviral strategies against Lassa fever with the aim of enhancing the IFN response...
  25. ncbi Sequence analysis of L RNA of Lassa virus
    Simon Vieth
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
    Virology 318:153-68. 2004
    ..In conclusion, the data provide a basis for structural and functional characterization of the Lassa virus L protein and reveal new insights into the phylogeny of negative-strand RNA viruses...
  26. ncbi Establishment of conventional and fluorescence resonance energy transfer-based real-time PCR assays for detection of pathogenic New World arenaviruses
    Simon Vieth
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Clin Virol 32:229-35. 2005
    ..Four of the viruses, namely Junin, Guanarito, Machupo, and Sabia, belong to clade B of New World arenaviruses that also comprises the nonpathogenic viruses Tacaribe, Cupixi, and Amapari...
  27. pmc Mutational analysis of the lassa virus promoter
    Meike Hass
    Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Virol 80:12414-9. 2006
    ..The first region appears to interact with the replication complex mainly via base-specific interactions, while in the second region solely base pairing between 3' and 5' promoter ends is important for promoter function...
  28. ncbi Molecular diagnostics of viral hemorrhagic fevers
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    Antiviral Res 57:61-87. 2003
    ..The third part is focussed on important differential diagnoses of VHF...
  29. pmc Domain structure of Lassa virus L protein
    Linda Brunotte
    Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Virol 85:324-33. 2011
    ..The first domain seems to mediate accumulation of L protein into cytoplasmic dot-like structures...
  30. pmc Broad-spectrum antiviral activity of small interfering RNA targeting the conserved RNA termini of Lassa virus
    Stefanie Müller
    Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    Antimicrob Agents Chemother 51:2215-8. 2007
    ....
  31. pmc Mutational evidence for a structural model of the Lassa virus RNA polymerase domain and identification of two residues, Gly1394 and Asp1395, that are critical for transcription but not replication of the genome
    Meike Hass
    Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Virol 82:10207-17. 2008
    ..A functional element within the RdRp was identified, which is important for transcription but not replication of the genome...
  32. ncbi Hospital-based surveillance for Lassa fever in Edo State, Nigeria, 2005-2008
    Deborah U Ehichioya
    Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria
    Trop Med Int Health 17:1001-4. 2012
    ..To estimate the burden of Lassa fever in northern and central Edo, a state in south Nigeria where Lassa fever has been reported...
  33. ncbi Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories
    Marcus Panning
    Bernhard Nocht Institute for Tropical Medicine, Hamburg 20359, Germany
    J Infect Dis 196:S199-204. 2007
    ..The assay facilitates reliable detection or exclusion screening of filovirus infections...
  34. ncbi Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro
    Stephan Gunther
    Department of Virology, Bernhard Nocht Institute of Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    Antiviral Res 63:209-15. 2004
    ..The IC(50) values of the most active compounds ranged from 5 to 21 micrograms/ml. The compounds also inhibited replication of SARS coronavirus and Ebola virus in analogous assays, although to a lesser extent than Lassa virus...
  35. pmc Inhibition of filovirus replication by the zinc finger antiviral protein
    Stefanie Müller
    Department of Virology, Bernhard Nocht Strasse 74, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
    J Virol 81:2391-400. 2007
    ..This inhibitory effect could be partially compensated for by overexpression of L protein. In conclusion, the data demonstrate that ZAP exhibits antiviral activity against filoviruses, presumably by decreasing the level of viral mRNA...
  36. ncbi A 10-year molecular survey of herpes simplex virus type 1 in Germany demonstrates a stable and high prevalence of genotypes A and B
    Jonas Schmidt-Chanasit
    Bernhard Nocht Institute for Tropical Medicine, Department of Virology, Hamburg, Germany
    J Clin Virol 44:235-7. 2009
    ..Recently three different herpes simplex virus type 1 (HSV-1) genotypes (A, B and C) were described on DNA sequence data of the glycoprotein E (gE), G (gG) and I (gI) genes...
  37. pmc Evaluation of advanced reverse transcription-PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome-associated coronavirus
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 42:2043-7. 2004
    ..Methods for the routine sampling of sputum without infection risk are needed to improve SARS RT-PCR...
  38. ncbi Sequence and phylogenetic analysis of hepatitis B virus genotype G isolated in Germany
    Simon Vieth
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Virus Genes 24:153-6. 2002
    ..7% identity) to both genotype G genomes (B1-89 and FR1 from France) described so far, suggesting either close epidemiological link among European genotype G isolates or high genetic stability of genotype G...
  39. ncbi Monitoring of clinical and laboratory data in two cases of imported Lassa fever
    Herbert Schmitz
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    Microbes Infect 4:43-50. 2002
    ..These data suggest that a high virus load and high levels of pro-inflammatory cytokines in the late stage of Lassa fever play an important role in the pathogenesis of hemorrhage, multi-organ failure, and shock in Lassa fever...
  40. pmc The N terminus of Andes virus L protein suppresses mRNA and protein expression in mammalian cells
    Patrick Heinemann
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Virol 87:6975-85. 2013
    ..Overall, these data suggest that ANDV L protein possesses a highly active endonuclease at the N terminus suppressing the level of its own as well as heterologous mRNAs upon recombinant expression in mammalian cells...
  41. pmc Structure of the Lassa virus nucleoprotein revealed by X-ray crystallography, small-angle X-ray scattering, and electron microscopy
    Linda Brunotte
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
    J Biol Chem 286:38748-56. 2011
    ..In conclusion, we describe the full-length crystal structure and the quaternary structure in solution of LASV NP. The nucleotide-binding pocket of NP could not be assigned a specific role in viral mRNA synthesis...
  42. doi Depletion of GTP pool is not the predominant mechanism by which ribavirin exerts its antiviral effect on Lassa virus
    Stephan Olschlager
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, 20359 Hamburg, Germany
    Antiviral Res 91:89-93. 2011
    ..However, this is not the predominant mechanism by which ribavirin exerts its in-vitro antiviral effect on Lassa virus...
  43. ncbi Strain-specific antibody response to Lassa virus in the local population of west Africa
    Petra Emmerich
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Street 74, D 20359 Hamburg, Germany
    J Clin Virol 42:40-4. 2008
    ..J Infect Dis 2001;184:345-9]. Therefore, three LAV strains from Sierra Leone, Ivory Coast and Nigeria were used as antigens to study the anti-LAV antibody response in 960 serum samples obtained from different regions of west Africa...
  44. ncbi Severe acute respiratory syndrome: identification of the etiological agent
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    Trends Mol Med 9:325-7. 2003
  45. ncbi Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases, Hamburg, Germany
    N Engl J Med 348:1967-76. 2003
    ..The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent...
  46. pmc Molecular analysis of varicella-zoster virus strains circulating in Tanzania demonstrating the presence of genotype M1
    Jonas Schmidt-Chanasit
    Bernhard Nocht Institute for Tropical Medicine, Diagnostic Virology Laboratory, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Clin Microbiol 46:3530-3. 2008
    ..Moreover, sequence analysis suggests recombination events between different VZV genotypes within ORFs 1, 31, 60, and 67...
  47. pmc Rapid and specific detection of Lassa virus by reverse transcription-PCR coupled with oligonucleotide array hybridization
    Stephan Olschlager
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 50:2496-9. 2012
    ..The array procedure may be performed with low-tech equipment and does not take longer than agarose gel detection...
  48. ncbi First outbreak of callitrichid hepatitis in Germany: genetic characterization of the causative lymphocytic choriomeningitis virus strains
    M Asper
    , Hamburg, Germany
    Virology 284:203-13. 2001
    ..These data show that CH is widespread and can be caused by distantly related LCMV strains...
  49. ncbi Functional analysis of hepatitis B virus reactivating in hepatitis B surface antigen-negative individuals
    Meike Hass
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Hepatology 42:93-103. 2005
    ..Supplementary material for this article can be found on the HEPATOLOGY website (http://www.interscience.wiley.com/jpages/0270-9139/suppmat/index.html)...
  50. pmc Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR
    Christian Drosten
    Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 40:2323-30. 2002
    ..6 to 16 RNA copies per assay). The suitability of the assays was exemplified by detection and quantification of viral RNA in serum samples of VHF patients...
  51. ncbi Analysis of gene expression in Lassa virus-infected HuH-7 cells
    Stefanie Müller
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany
    J Gen Virol 88:1568-75. 2007
    ..In conclusion, Lassa virus interferes only minimally with gene expression in HuH-7 cells and poorly induces IFN-beta gene transcription...
  52. ncbi Role of the C terminus of lassa virus L protein in viral mRNA synthesis
    Maria Lehmann
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Virol 88:8713-7. 2014
    ..Thus, the C terminus of L protein is involved in the mRNA synthesis process, potentially by mediating cap binding. ..
  53. ncbi Increase in West Nile virus infections imported to Germany in 2012
    Martin Gabriel
    Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Hamburg, Germany
    J Clin Virol 58:587-9. 2013
    ..In this report we summarize the clinical and laboratory findings in order to sensitize physicians in Germany for this imported viral disease. ..
  54. pmc Evaluation of antiviral efficacy of ribavirin, arbidol, and T-705 (favipiravir) in a mouse model for crimean-congo hemorrhagic Fever
    Lisa Oestereich
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany German Centre for Infection Research DZIF, Hamburg, Germany
    PLoS Negl Trop Dis 8:e2804. 2014
    ..We aimed at characterizing the liver pathology in CCHF virus-infected IFNAR-/- mice by immunohistochemistry and employed the model to evaluate the antiviral efficacy of ribavirin, arbidol, and T-705 against CCHF virus...
  55. pmc Laboratory diagnosis of lassa Fever, liberia
    Marcus Panning
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany M Panning, P Emmerich, S Olschlager, S Gunther, C Drosten United Nations Mission in Sierra Leone, Freetown, Sierra Leone S Bojenko Program on Hemorrhagic Fevers, Conakry, Guinea L Koivogui World Health Organization, Geneva, Switzerland A Marx World Health Organization, Monrovia, Liberia P C Lugala and Tulane University Health Sciences Center, New Orleans, Louisiana, USA D G Bausch
    Emerg Infect Dis 16:1041-3. 2010
    ..Reverse transcription-PCR (RT-PCR) is routinely used for confirmation of cases, but few proven assay formulations are available, and these have not been evaluated on larger cohorts of patients (5)...
  56. ncbi Functional analysis of HBV genomes from patients with fulminant hepatitis
    M Sterneck
    Department of Medicine, University Hospital Hamburg Eppendorf, Germany
    Hepatology 28:1390-7. 1998
    ..However, neither phenotype is an essential prerequisite; thus, an additional role of other viral or host factors in the pathogenesis of FHB is suggested...
  57. pmc Amplification of full-length hepatitis B virus genomes from samples from patients with low levels of viremia: frequency and functional consequences of PCR-introduced mutations
    S Gunther
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Federal Republic of Germany
    J Clin Microbiol 36:531-8. 1998
    ..Because replication competence is highly sensitive to random mutations, it is the best marker for the identification of HBV genomes with few or no PCR-introduced mutations...
  58. ncbi Neonatal fulminant hepatitis B: structural and functional analysis of complete hepatitis B virus genomes from mother and infant
    M Sterneck
    Department of Medicine, Surgery, and Pediatrics, University Hospital Eppendorf, Hamburg, Germany
    J Infect Dis 177:1378-81. 1998
    ..In this case, a de novo infection with variants showing a defect in HBe antigen and pre-S2 protein synthesis but not a high replication competence probably contributed to the fulminant disease course...
  59. pmc Imported lassa fever in Germany: molecular characterization of a new lassa virus strain
    S Gunther
    Bernhard Nocht Institut fur Tropenmedizin, Hamburg, Germany
    Emerg Infect Dis 6:466-76. 2000
    ..The new method for full-length S RNA amplification may facilitate identification and molecular analysis of new arenaviruses or arenavirus strains...
  60. ncbi Reactivation of hepatitis B virus replication accompanied by acute hepatitis in patients receiving highly active antiretroviral therapy
    C Manegold
    Klinische Abteilung, Bernhard Nocht Institut fur Tropenmedizin, Hamburg, Germany
    Clin Infect Dis 32:144-8. 2001
    ..We found that reactivation of HBV replication during HAART can occur independently of lamivudine resistance or withdrawal of lamivudine, and in spite of increasing CD4(+) cell counts...
  61. ncbi Wild-type levels of pregenomic RNA and replication but reduced pre-C RNA and e-antigen synthesis of hepatitis B virus with C(1653) --> T, A(1762) --> T and G(1764) --> A mutations in the core promoter
    S Gunther
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Federal Republic of Germany
    J Gen Virol 79:375-80. 1998
    ..The amount of progeny virus DNA in the cells and in the culture medium was increased marginally, if at all...
  62. pmc Infection of type I interferon receptor-deficient mice with various old world arenaviruses: a model for studying virulence and host species barriers
    Toni Rieger
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS ONE 8:e72290. 2013
    ..The observation that Lassa virus overcomes the species barrier without artificial depletion of T cells suggests it is able to impair T cell functionality in a way that corresponds to depletion. ..
  63. ncbi Successful treatment of advanced Ebola virus infection with T-705 (favipiravir) in a small animal model
    Lisa Oestereich
    Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany German Centre for Infection Research DZIF, partner site Hamburg, Germany
    Antiviral Res 105:17-21. 2014
    ..The findings suggest that T-705 is a candidate for treatment of Ebola hemorrhagic fever. ..
  64. pmc Lambda interferon renders epithelial cells of the respiratory and gastrointestinal tracts resistant to viral infections
    Markus Mordstein
    Department of Virology, University of Freiburg, Hermann Herder Strasse 11, D 79104 Freiburg, Germany
    J Virol 84:5670-7. 2010
    ....