Adam Grundhoff

Summary

Affiliation: University of Hamburg
Country: Germany

Publications

  1. doi request reprint Merkel cell polyomavirus, a highly prevalent virus with tumorigenic potential
    Adam Grundhoff
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Department Virus Genomics, Martinistrasse 52, 20252 Hamburg, Germany Electronic address
    Curr Opin Virol 14:129-37. 2015
  2. doi request reprint Computational prediction of viral miRNAs
    Adam Grundhoff
    Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
    Methods Mol Biol 721:143-52. 2011
  3. pmc Virus-encoded microRNAs
    Adam Grundhoff
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistr, Hamburg, Germany
    Virology 411:325-43. 2011
  4. pmc A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses
    Adam Grundhoff
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Germany
    RNA 12:733-50. 2006
  5. doi request reprint MicroRNA profiling during human keratinocyte differentiation using a quantitative real-time PCR method
    Janosch Hildebrand
    R and D, Skin Research Center, Beiersdorf AG, Hamburg, Germany
    Methods Mol Biol 961:193-200. 2013
  6. doi request reprint A Comprehensive Analysis of Replicating Merkel Cell Polyomavirus Genomes Delineates the Viral Transcription Program and Suggests a Role for mcv-miR-M1 in Episomal Persistence
    Juliane Marie Theiss
    Research Group Virus Genomics, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    PLoS Pathog 11:e1004974. 2015
  7. pmc High-affinity Rb binding, p53 inhibition, subcellular localization, and transformation by wild-type or tumor-derived shortened Merkel cell polyomavirus large T antigens
    Sophie Borchert
    Institute for Medical Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
    J Virol 88:3144-60. 2014
  8. pmc A global analysis of evolutionary conservation among known and predicted gammaherpesvirus microRNAs
    Nicole Walz
    Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse 52, D 20251 Hamburg, Germany
    J Virol 84:716-28. 2010
  9. doi request reprint Evaluation of Unbiased Next-Generation Sequencing of RNA (RNA-seq) as a Diagnostic Method in Influenza Virus-Positive Respiratory Samples
    Nicole Fischer
    Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf UKE, Hamburg, Germany German Center for Infection research DZIF, partner site Hamburg Borstel Lübeck, Germany
    J Clin Microbiol 53:2238-50. 2015
  10. doi request reprint In Vitro Replication Assay for Merkel Cell Polyomavirus (MCPyV)
    Friederike Neumann
    Institute for Medical Microbiology, Virology and Hygiene University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Curr Protoc Microbiol 38:14F.2.1-14F.2.19. 2015

Collaborators

  • Nicole Fischer
  • Frank Buchholz
  • Joachim Hauber
  • Thomas Dobner
  • Anja Knott
  • Thomas Meyer
  • Jesper Salomon
  • Martin Trepel
  • Thomas Rattei
  • Ingrid Moll
  • Philip Hartjen
  • Markus G Manz
  • Martin Aepfelbacher
  • Udo Schumacher
  • Axel Schambach
  • Jan Chemnitz
  • DON E GANEM
  • Jill Bechtel
  • Malik Alawi
  • Friederike Neumann
  • Thomas Gunther
  • Nicole Walz
  • Janosch Hildebrand
  • Sophie Borchert
  • Uwe Tessmer
  • Juliane Marie Theiss
  • Friederike Braig
  • Daniela Indenbirken
  • Mascha Binder
  • Manja Czech-Sioli
  • Nele Ilmberger
  • Benjamin Thiele
  • Miriam Leitz
  • Simon Güllert
  • Wolfgang R Streit
  • Christel Schmeisser
  • Rolf Daniel
  • Bernd Wemheuer
  • Anja Poehlein
  • Ilona Hauber
  • Birte Niebuhr
  • Ines Krohn-Molt
  • Claudia Schmidt
  • Carol Stocking
  • Helga Hofmann-Sieber
  • Horst Wenck
  • Stefan Gallinat
  • Lakshmikanth Mariyanna
  • Christine Dahlke
  • Thomas Christalla
  • Christopher S Sullivan
  • Joseph Ziegelbauer
  • Alexander Stein
  • Manuela Marz
  • Alexander Schulte
  • Sonja Loges
  • Malte Kriegs
  • Carsten Bokemeyer
  • Mareike Voigt
  • Aneta Schieferdecker
  • Tobias Grob
  • Udo Vanhoefer
  • Kristoffer Riecken
  • Boris Fehse
  • Erik Engel
  • Joana Dannenberg
  • Martin S Borchert
  • Johanna M Brandner
  • Ulrich Rabausch
  • Kristin Stieler
  • Peter B Olsen
  • Jeremy Torres
  • Sabrina Schreiner
  • Marie Kloster
  • Jennifer Chow
  • Dimitrij Turaev
  • Peter Wimmer
  • Janet Chusainow
  • Neele Kriebitzsch
  • Kira Behrens
  • Patrick Ziegler
  • Andreas Pommerening-Röser
  • Dieter Hanelt
  • Christoph Lindner
  • Karl Hackmann
  • Evelin Schrock
  • Ursula Muller
  • Ulla Bergholz
  • Marion Ziegler
  • Danilo Dubrau

Detail Information

Publications28

  1. doi request reprint Merkel cell polyomavirus, a highly prevalent virus with tumorigenic potential
    Adam Grundhoff
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Department Virus Genomics, Martinistrasse 52, 20252 Hamburg, Germany Electronic address
    Curr Opin Virol 14:129-37. 2015
    ..This review will carve out the specifics of MCPyV biology and discuss unresolved issues to help the reader gain a better understanding of what may differentiate MCPyV from other polyomaviruses. ..
  2. doi request reprint Computational prediction of viral miRNAs
    Adam Grundhoff
    Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
    Methods Mol Biol 721:143-52. 2011
    ..Here, we provide a detailed protocol for the prediction of putative miRNA genes using VMir, an ab initio prediction program which we have recently designed specifically to identify pre-miRNAs in viral genomes...
  3. pmc Virus-encoded microRNAs
    Adam Grundhoff
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistr, Hamburg, Germany
    Virology 411:325-43. 2011
    ..Key steps forward for the field are discussed, including the need for additional functional studies that utilize surgical viral miRNA mutants combined with relevant models of infection...
  4. pmc A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses
    Adam Grundhoff
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Germany
    RNA 12:733-50. 2006
    ..The advantages and limitations of the approach are discussed...
  5. doi request reprint MicroRNA profiling during human keratinocyte differentiation using a quantitative real-time PCR method
    Janosch Hildebrand
    R and D, Skin Research Center, Beiersdorf AG, Hamburg, Germany
    Methods Mol Biol 961:193-200. 2013
    ..Here, we describe a general real-time PCR method for miRNA profiling using isolated epidermal stem cells, transiently amplifying cells and terminally differentiated keratinocytes of human skin...
  6. doi request reprint A Comprehensive Analysis of Replicating Merkel Cell Polyomavirus Genomes Delineates the Viral Transcription Program and Suggests a Role for mcv-miR-M1 in Episomal Persistence
    Juliane Marie Theiss
    Research Group Virus Genomics, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    PLoS Pathog 11:e1004974. 2015
    ..Our data suggest that MCPyV may have evolved strategies to enter a non- or low level vegetative stage of infection which could aid the virus in establishing and maintaining a lifelong persistence. ..
  7. pmc High-affinity Rb binding, p53 inhibition, subcellular localization, and transformation by wild-type or tumor-derived shortened Merkel cell polyomavirus large T antigens
    Sophie Borchert
    Institute for Medical Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
    J Virol 88:3144-60. 2014
    ....
  8. pmc A global analysis of evolutionary conservation among known and predicted gammaherpesvirus microRNAs
    Nicole Walz
    Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse 52, D 20251 Hamburg, Germany
    J Virol 84:716-28. 2010
    ..Although several other gammaherpesviruses are predicted to encode large numbers of clustered miRNAs at conserved genomic loci, no further examples of evolutionarily conserved miRNA sequences were found...
  9. doi request reprint Evaluation of Unbiased Next-Generation Sequencing of RNA (RNA-seq) as a Diagnostic Method in Influenza Virus-Positive Respiratory Samples
    Nicole Fischer
    Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf UKE, Hamburg, Germany German Center for Infection research DZIF, partner site Hamburg Borstel Lübeck, Germany
    J Clin Microbiol 53:2238-50. 2015
    ..Thus, analysis of respiratory samples from known or suspected influenza patients by UMERS provides valuable information that is relevant for clinical investigation. ..
  10. doi request reprint In Vitro Replication Assay for Merkel Cell Polyomavirus (MCPyV)
    Friederike Neumann
    Institute for Medical Microbiology, Virology and Hygiene University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Curr Protoc Microbiol 38:14F.2.1-14F.2.19. 2015
    ..However, serial transmission of infectious virus is still restricted in these cells. © 2015 by John Wiley & Sons, Inc. ..
  11. pmc A microRNA encoded by Kaposi sarcoma-associated herpesvirus promotes B-cell expansion in vivo
    Christine Dahlke
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS ONE 7:e49435. 2012
    ....
  12. pmc A comparative metagenome survey of the fecal microbiota of a breast- and a plant-fed Asian elephant reveals an unexpectedly high diversity of glycoside hydrolase family enzymes
    Nele Ilmberger
    Universitat Hamburg, Biozentrum Klein Flottbek, Abteilung für Mikrobiologie and Biotechnologie, Hamburg, Germany
    PLoS ONE 9:e106707. 2014
    ..Altogether, this study delivers insight into the biomass conversion by one of the largest plant-fed and land-living animals. ..
  13. pmc Influence of ND10 components on epigenetic determinants of early KSHV latency establishment
    Thomas Gunther
    Research Group Virus Genomics, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS Pathog 10:e1004274. 2014
    ....
  14. pmc The epigenetic landscape of latent Kaposi sarcoma-associated herpesvirus genomes
    Thomas Gunther
    Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
    PLoS Pathog 6:e1000935. 2010
    ..Our findings suggest that the modification patterns identified here induce a poised state of repression during viral latency, which can be rapidly reversed once the lytic cycle is induced...
  15. pmc Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome
    Friederike Neumann
    Institute for Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
    PLoS ONE 6:e29112. 2011
    ..However, serial transmission of infectious virus was not observed. This in vitro culturing system allows the study of viral replication and will facilitate the molecular dissection of important aspects of the MCPyV lifecycle...
  16. doi request reprint Next-generation sequencing of peripheral B-lineage cells pinpoints the circulating clonotypic cell pool in multiple myeloma
    Benjamin Thiele
    University Medical Center Hamburg Eppendorf, Hubertus Wald Tumorzentrum, University Cancer Center Hamburg, Department of Oncology, Hematology, Bone Marrow Transplantation with Section Pneumology, Hamburg, Germany
    Blood 123:3618-21. 2014
    ..Our findings consistently show an absence of pre-switch clonotypic B cells, while M-PCs circulate in the peripheral blood and may contribute to spreading of the disease. ..
  17. pmc Highly significant antiviral activity of HIV-1 LTR-specific tre-recombinase in humanized mice
    Ilona Hauber
    Heinrich Pette Institute Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS Pathog 9:e1003587. 2013
    ..Taken together, our data support the use of Tre-recombinase in novel therapy strategies aiming to provide a cure for HIV...
  18. doi request reprint Runx1 is essential at two stages of early murine B-cell development
    Birte Niebuhr
    Molecular Pathology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, Hamburg, Germany
    Blood 122:413-23. 2013
    ....
  19. doi request reprint A comprehensive analysis of microRNA expression during human keratinocyte differentiation in vitro and in vivo
    Janosch Hildebrand
    Beiersdorf AG, R and D, Skin Research Center, Hamburg, Germany
    J Invest Dermatol 131:20-9. 2011
    ..Our results thus provide the basis for further analysis of miRNA functions during epidermal differentiation...
  20. ncbi request reprint Epidermal growth factor receptor mutation mediates cross-resistance to panitumumab and cetuximab in gastrointestinal cancer
    Friederike Braig
    Department of Oncology and Hematology, BMT With Section Pneumology, Hubertus Wald Tumorzentrum UCCH, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Oncotarget 6:12035-47. 2015
    ....
  21. doi request reprint Merkel cell polyomavirus detection in Merkel cell cancer tumors in Northern Germany using PCR and protein expression
    Miriam Leitz
    Institute for Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
    J Med Virol 86:1813-9. 2014
    ..These data are in accord with published studies describing heterogeneity in MCPyV viral loads and suggest that detection of MCPyV in Merkel cell carcinoma by PCR should be undertaken using multiple primer pairs...
  22. pmc Metagenome survey of a multispecies and alga-associated biofilm revealed key elements of bacterial-algal interactions in photobioreactors
    Ines Krohn-Molt
    University of Hamburg, Biocenter Klein Flottbek, Microbiology and Biotechnology, Hamburg, Germany
    Appl Environ Microbiol 79:6196-206. 2013
    ..This is the first metagenome study of microalga- and PBR-associated biofilm bacteria, and it gives new clues for improved biofuel production in PBRs. ..
  23. pmc Excision of HIV-1 proviral DNA by recombinant cell permeable tre-recombinase
    Lakshmikanth Mariyanna
    Heinrich Pette Institute Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS ONE 7:e31576. 2012
    ..The presented data confirm Tre-recombinase activity on integrated HIV-1 and provide the basis for the non-genetic transient application of engineered recombinases, which may be a valuable component of future HIV eradication strategies...
  24. ncbi request reprint Identification of viral microRNAs
    Christopher S Sullivan
    Department of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas, USA
    Methods Enzymol 427:3-23. 2007
    ..Here, we provide a detailed protocol on how to use computational prediction methods in conjunction with high-throughput microarray analysis to detect miRNAs in viral genomes...
  25. pmc Inefficient establishment of KSHV latency suggests an additional role for continued lytic replication in Kaposi sarcoma pathogenesis
    Adam Grundhoff
    Howard Hughes Medical Institute and Department of Microbiology, University of California, San Francisco, San Francisco, California 94143 0414, USA
    J Clin Invest 113:124-36. 2004
    ..They also suggest an additional role for lytic replication in sustaining KS tumorigenesis: namely, the recruitment of new cells to latency to replace those that have segregated the viral episome...
  26. pmc RNAs in the virion of Kaposi's sarcoma-associated herpesvirus
    Jill Bechtel
    Howard Hughes Medical Institute and G W Hooper Foundation, University of California, San Francisco, CA 94143, USA
    J Virol 79:10138-46. 2005
    ..However, the intracellular levels of one virion mRNA, encoding the viral protease, were much lower than those of transcripts not packaged in the virus particle, strongly suggesting that it may be incorporated by a specific mechanism...
  27. pmc Exploring the DNA binding interactions of the Kaposi's sarcoma-associated herpesvirus lytic switch protein by selective amplification of bound sequences in vitro
    Joseph Ziegelbauer
    G W Hooper Foundation, University of California, San Francisco, Box 0552, 513 Parnassus Ave, San Francisco, California 94143 0552, USA
    J Virol 80:2958-67. 2006
    ..This raises the possibility that some late KSHV genes may also be subject to direct RTA regulation, though indirect models are not excluded...
  28. pmc The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus permits replication of terminal repeat-containing plasmids
    Adam Grundhoff
    Departments of Microbiology and Medicine, Howard Hughes Medical Institute, University of California Medical Center, San Francisco, CA 94143 0414, USA
    J Virol 77:2779-83. 2003
    ....