Research Topics
| Adam GrundhoffSummaryAffiliation: University of Hamburg Country: Germany Publications
| Collaborators
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Detail Information
Publications
Computational prediction of viral miRNAsAdam Grundhoff
Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
Methods Mol Biol 721:143-52. 2011..Here, we provide a detailed protocol for the prediction of putative miRNA genes using VMir, an ab initio prediction program which we have recently designed specifically to identify pre-miRNAs in viral genomes...
Virus-encoded microRNAsAdam Grundhoff
Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistr, Hamburg, Germany
Virology 411:325-43. 2011..Key steps forward for the field are discussed, including the need for additional functional studies that utilize surgical viral miRNA mutants combined with relevant models of infection...- A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesvirusesAdam Grundhoff
Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Germany
RNA 12:733-50. 2006..The advantages and limitations of the approach are discussed... - MicroRNA profiling during human keratinocyte differentiation using a quantitative real-time PCR methodJanosch Hildebrand
R and D, Skin Research Center, Beiersdorf AG, Hamburg, Germany
Methods Mol Biol 961:193-200. 2013..Here, we describe a general real-time PCR method for miRNA profiling using isolated epidermal stem cells, transiently amplifying cells and terminally differentiated keratinocytes of human skin... - A global analysis of evolutionary conservation among known and predicted gammaherpesvirus microRNAsNicole Walz
Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse 52, D 20251 Hamburg, Germany
J Virol 84:716-28. 2010..Although several other gammaherpesviruses are predicted to encode large numbers of clustered miRNAs at conserved genomic loci, no further examples of evolutionarily conserved miRNA sequences were found... - The epigenetic landscape of latent Kaposi sarcoma-associated herpesvirus genomesThomas Gunther
Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
PLoS Pathog 6:e1000935. 2010..Our findings suggest that the modification patterns identified here induce a poised state of repression during viral latency, which can be rapidly reversed once the lytic cycle is induced... - A microRNA encoded by Kaposi sarcoma-associated herpesvirus promotes B-cell expansion in vivoChristine Dahlke
Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
PLoS ONE 7:e49435. 2012.... - A comprehensive analysis of microRNA expression during human keratinocyte differentiation in vitro and in vivoJanosch Hildebrand
Beiersdorf AG, R and D, Skin Research Center, Hamburg, Germany
J Invest Dermatol 131:20-9. 2011..Our results thus provide the basis for further analysis of miRNA functions during epidermal differentiation... - Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genomeFriederike Neumann
Institute for Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
PLoS ONE 6:e29112. 2011..However, serial transmission of infectious virus was not observed. This in vitro culturing system allows the study of viral replication and will facilitate the molecular dissection of important aspects of the MCPyV lifecycle... - Excision of HIV-1 proviral DNA by recombinant cell permeable tre-recombinaseLakshmikanth Mariyanna
Heinrich Pette Institute Leibniz Institute for Experimental Virology, Hamburg, Germany
PLoS ONE 7:e31576. 2012..The presented data confirm Tre-recombinase activity on integrated HIV-1 and provide the basis for the non-genetic transient application of engineered recombinases, which may be a valuable component of future HIV eradication strategies... 
Identification of viral microRNAsChristopher S Sullivan
Department of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas, USA
Methods Enzymol 427:3-23. 2007..Here, we provide a detailed protocol on how to use computational prediction methods in conjunction with high-throughput microarray analysis to detect miRNAs in viral genomes...- Exploring the DNA binding interactions of the Kaposi's sarcoma-associated herpesvirus lytic switch protein by selective amplification of bound sequences in vitroJoseph Ziegelbauer
G.W. Hooper Foundation, University of California, San Francisco, Box 0552, 513 Parnassus Ave, San Francisco, California 94143-0552, USA
J Virol 80:2958-67. 2006..This raises the possibility that some late KSHV genes may also be subject to direct RTA regulation, though indirect models are not excluded... - RNAs in the virion of Kaposi's sarcoma-associated herpesvirusJill Bechtel
Howard Hughes Medical Institute and G W Hooper Foundation, University of California, San Francisco, CA 94143, USA
J Virol 79:10138-46. 2005..However, the intracellular levels of one virion mRNA, encoding the viral protease, were much lower than those of transcripts not packaged in the virus particle, strongly suggesting that it may be incorporated by a specific mechanism... - Inefficient establishment of KSHV latency suggests an additional role for continued lytic replication in Kaposi sarcoma pathogenesisAdam Grundhoff
Howard Hughes Medical Institute and Department of Microbiology, University of California, San Francisco, San Francisco, California 94143-0414, USA
J Clin Invest 113:124-36. 2004..They also suggest an additional role for lytic replication in sustaining KS tumorigenesis: namely, the recruitment of new cells to latency to replace those that have segregated the viral episome... - The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus permits replication of terminal repeat-containing plasmidsAdam Grundhoff
Departments of Microbiology and Medicine, Howard Hughes Medical Institute, University of California Medical Center, San Francisco, CA 94143-0414, USA
J Virol 77:2779-83. 2003....