Marcus Groettrup

Summary

Affiliation: University of Konstanz
Country: Germany

Publications

  1. ncbi request reprint An altered T cell repertoire in MECL-1-deficient mice
    Michael Basler
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    J Immunol 176:6665-72. 2006
  2. pmc Immuno- and constitutive proteasomes do not differ in their abilities to degrade ubiquitinated proteins
    James A Nathan
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 152:1184-94. 2013
  3. pmc Inhibition of the immunoproteasome ameliorates experimental autoimmune encephalomyelitis
    Michael Basler
    Biotechnology Institute Thurgau BITg at the University of Konstanz, Kreuzlingen, Switzerland
    EMBO Mol Med 6:226-38. 2014
  4. doi request reprint Proteasomes in immune cells: more than peptide producers?
    Marcus Groettrup
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    Nat Rev Immunol 10:73-8. 2010
  5. doi request reprint Reduced immunoproteasome formation and accumulation of immunoproteasomal precursors in the brains of lymphocytic choriomeningitis virus-infected mice
    Marcel Kremer
    Division of Immunology, Department of Biology, Constance University, Konstanz, Germany
    J Immunol 185:5549-60. 2010
  6. doi request reprint Immunoproteasomes are essential for survival and expansion of T cells in virus-infected mice
    Jacqueline Moebius
    Division of Immunology, Department of Biology, Constance University, Konstanz, Germany
    Eur J Immunol 40:3439-49. 2010
  7. doi request reprint Attenuation of the cytotoxic T lymphocyte response to lymphocytic choriomeningitis virus in mice subjected to chronic social stress
    Annette Sommershof
    Division of Immunology, Department of Biology, Constance University, D 78457 Konstanz, Germany
    Brain Behav Immun 25:340-8. 2011
  8. doi request reprint CD8- dendritic cells and macrophages cross-present poly(D,L-lactate-co-glycolate) acid microsphere-encapsulated antigen in vivo
    Christopher Schliehe
    Division of Immunology, Department of Biology, Constance University, 78457 Konstanz, Germany
    J Immunol 187:2112-21. 2011
  9. ncbi request reprint No essential role for tripeptidyl peptidase II for the processing of LCMV-derived T cell epitopes
    Michael Basler
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    Eur J Immunol 37:896-904. 2007
  10. pmc Stable antigen is most effective for eliciting CD8+ T-cell responses after DNA vaccination and infection with recombinant vaccinia virus in vivo
    Christopher Schliehe
    Division of Immunology, Department of Biology, Constance University, Konstanz, Germany
    J Virol 86:9782-93. 2012

Collaborators

Detail Information

Publications41

  1. ncbi request reprint An altered T cell repertoire in MECL-1-deficient mice
    Michael Basler
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    J Immunol 176:6665-72. 2006
    ..Taken together, our data reveal an in vivo function of MECL-1 in codetermining the T cell repertoire for an antiviral CTL response...
  2. pmc Immuno- and constitutive proteasomes do not differ in their abilities to degrade ubiquitinated proteins
    James A Nathan
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 152:1184-94. 2013
    ..We conclude that, although immunoproteasomes can increase the generation of peptides appropriate for MHC class I presentation, they do not degrade ubiquitinated proteins more efficiently than constitutive particles...
  3. pmc Inhibition of the immunoproteasome ameliorates experimental autoimmune encephalomyelitis
    Michael Basler
    Biotechnology Institute Thurgau BITg at the University of Konstanz, Kreuzlingen, Switzerland
    EMBO Mol Med 6:226-38. 2014
    ..These results implicate the immunoproteasome in the development of EAE and suggest that immunoproteasome inhibitors are promising drugs for the treatment of MS. ..
  4. doi request reprint Proteasomes in immune cells: more than peptide producers?
    Marcus Groettrup
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    Nat Rev Immunol 10:73-8. 2010
    ..We also discuss how inhibitors of immunoproteasomes can suppress undesired T cell responses in autoimmune diseases...
  5. doi request reprint Reduced immunoproteasome formation and accumulation of immunoproteasomal precursors in the brains of lymphocytic choriomeningitis virus-infected mice
    Marcel Kremer
    Division of Immunology, Department of Biology, Constance University, Konstanz, Germany
    J Immunol 185:5549-60. 2010
    ....
  6. doi request reprint Immunoproteasomes are essential for survival and expansion of T cells in virus-infected mice
    Jacqueline Moebius
    Division of Immunology, Department of Biology, Constance University, Konstanz, Germany
    Eur J Immunol 40:3439-49. 2010
    ..Therefore, the immunoproteasome may qualify as a potential new target for the suppression of undesired proinflammatory T-cell responses...
  7. doi request reprint Attenuation of the cytotoxic T lymphocyte response to lymphocytic choriomeningitis virus in mice subjected to chronic social stress
    Annette Sommershof
    Division of Immunology, Department of Biology, Constance University, D 78457 Konstanz, Germany
    Brain Behav Immun 25:340-8. 2011
    ..Taken together, this study shows that chronic social stress fundamentally suppresses the functional capacities of T cells during a viral infection...
  8. doi request reprint CD8- dendritic cells and macrophages cross-present poly(D,L-lactate-co-glycolate) acid microsphere-encapsulated antigen in vivo
    Christopher Schliehe
    Division of Immunology, Department of Biology, Constance University, 78457 Konstanz, Germany
    J Immunol 187:2112-21. 2011
    ..Moreover, immune responses in BATF3 knockout mice lacking CD8(+) DCs were normal. The results presented in this work strongly suggest that in vivo cross-priming of PLGA MS-encapsulated Ag is performed by CD8(-) DCs and MΦs...
  9. ncbi request reprint No essential role for tripeptidyl peptidase II for the processing of LCMV-derived T cell epitopes
    Michael Basler
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    Eur J Immunol 37:896-904. 2007
    ..We propose that TPPII is not generally required for the production of MHC class I peptides, but the presentation of some peptides can be negatively affected by TPPII...
  10. pmc Stable antigen is most effective for eliciting CD8+ T-cell responses after DNA vaccination and infection with recombinant vaccinia virus in vivo
    Christopher Schliehe
    Division of Immunology, Department of Biology, Constance University, Konstanz, Germany
    J Virol 86:9782-93. 2012
    ..Hence, our results suggest a dominant role for antigen cross-priming in DNA vaccination and recombinant VV infection...
  11. doi request reprint TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses
    Eva Schlosser
    Division of Immunology, Department of Biology, Konstanz, Germany
    Vaccine 26:1626-37. 2008
    ..Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses...
  12. ncbi request reprint Prevention of experimental colitis by a selective inhibitor of the immunoproteasome
    Michael Basler
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    J Immunol 185:634-41. 2010
    ..Taken together, these results demonstrate that an immunoproteasome-specific inhibitor can be used to attenuate autoimmune diseases like colitis...
  13. pmc An artificial PAP gene breaks self-tolerance and promotes tumor regression in the TRAMP model for prostate carcinoma
    Elmar Spies
    Division of Immunology, Department of Biology, University of Constance, Constance, Germany
    Mol Ther 20:555-64. 2012
    ..Therefore, this study highlights a novel cancer immunotherapy approach with the potential to control PCa in clinical settings...
  14. doi request reprint FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis
    Neha Rani
    Division of Immunology, Department of Biology, University of Constance, 78457 Konstanz, Germany
    Nat Commun 3:749. 2012
    ..In conclusion, we identify the VWA domain of hRpn10 as a receptor for ubiquitin-like proteins within the 26S proteasome and elucidate how FAT10 mediates efficient proteolysis by the proteasome...
  15. ncbi request reprint Identification of homozygous transgenic mice by genomic real-time PCR
    Gunter Schmidtke
    Department of Biology, University of Konstanz, Konstanz, Germany
    Methods Mol Biol 429:45-58. 2008
    ..Here, we describe the analysis of these mice by quantitative PCR using sequence specific fluorescence resonance energy transfer-primers to reliably detect a difference in DNA content as small as a factor of 2 or only one PCR cycle...
  16. doi request reprint Degradation of FAT10 by the 26S proteasome is independent of ubiquitylation but relies on NUB1L
    Gunter Schmidtke
    Department of Biology, Division of Immunology, University of Constance, Konstanz, Germany
    FEBS Lett 583:591-4. 2009
    ..In conclusion, our data establish FAT10 as a ubiquitin-independent but NUB1L-dependent targeting signal for proteasomal degradation...
  17. doi request reprint Immunoproteasome subunit LMP7 deficiency and inhibition suppresses Th1 and Th17 but enhances regulatory T cell differentiation
    Khalid W Kalim
    Division of Immunology, Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    J Immunol 189:4182-93. 2012
    ..LMP7 governs Th cell lineage determination by affecting the balance of receptor proximal signals during differentiation. These data render LMP7 a promising drug target for the treatment of autoimmune diseases...
  18. doi request reprint Coencapsulation of tumor lysate and CpG-ODN in PLGA-microspheres enables successful immunotherapy of prostate carcinoma in TRAMP mice
    Marc Mueller
    Division of Immunology, Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    J Control Release 162:159-66. 2012
    ..Since PLGA is approved for clinical application, sterilized PLGA-MS containing tumor lysates and TLR ligands hold promise as anti-tumor vaccines against prostate carcinoma in humans...
  19. doi request reprint Tumor eradication by immunotherapy with biodegradable PLGA microspheres--an alternative to incomplete Freund's adjuvant
    Marc Mueller
    Division of Immunology, Department of Biology, Universitat Konstanz, Konstanz, Germany
    Int J Cancer 129:407-16. 2011
    ....
  20. ncbi request reprint Cross-presentation of the long-lived lymphocytic choriomeningitis virus nucleoprotein does not require neosynthesis and is enhanced via heat shock proteins
    Sameh Basta
    Department of Biology, Division of Immunology, University of Constance, Konstanz, Germany
    J Immunol 175:796-805. 2005
    ..We propose that cross-presentation has evolved to sustain the presentation of stable viral proteins when their neosynthesis has ceased in infected donor cells...
  21. pmc Posttraumatic stress disorder is associated with an enhanced spontaneous production of pro-inflammatory cytokines by peripheral blood mononuclear cells
    Hannah Gola
    Clinical Psychology and Neuropsychology, University of Konstanz, Konstanz, Germany
    BMC Psychiatry 13:40. 2013
    ..Posttraumatic stress disorder (PTSD) is associated with an enhanced risk for cardiovascular and other inflammatory diseases. Chronic low-level inflammation has been suggested as a potential mechanism linking these conditions...
  22. pmc A novel cytosolic class I antigen-processing pathway for endoplasmic-reticulum-targeted proteins
    Eva Schlosser
    Division of Immunology, Department of Biology, Postbox M661, Universitatstrasse 10, University of Constance, Konstanz D 78457, Germany
    EMBO Rep 8:945-51. 2007
    ....
  23. ncbi request reprint The ubiquitin-like modifier FAT10 interacts with HDAC6 and localizes to aggresomes under proteasome inhibition
    Birte Kalveram
    Department of Biology, Division of Immunology, University of Constance, Universitatsstrasse 10, 78457 Konstanz, Germany
    J Cell Sci 121:4079-88. 2008
    ....
  24. doi request reprint The immunoproteasome in antigen processing and other immunological functions
    Michael Basler
    Division of Immunology, Department of Biology, University of Konstanz, Universitatsstrasse 10, D 78457 Konstanz, Germany
    Curr Opin Immunol 25:74-80. 2013
    ..The recent elucidation of the high-resolution structure of the immunoproteasome will facilitate the design of immunoproteasome selective inhibitors for pharmacological intervention...
  25. doi request reprint Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit
    Khalid W Kalim
    Division of Immunology, Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    Mol Immunol 53:274-82. 2013
    ..Our data may help to reconcile controversial point of views on the pro- and anti-inflammatory nature of PGE2 by making a strong case for a cell type-dependent function...
  26. doi request reprint Immunoproteasome-specific inhibitors and their application
    Michael Basler
    Department of Biology, Division of Immunology, University of Konstanz, Konstanz, Germany
    Methods Mol Biol 832:391-401. 2012
    ..Thereby, we provide the methodology to analyse the specificity and cell permeability of immunoproteasome inhibitors, as well as to functionally investigate immunoproteasome inhibitors in antigen presentation...
  27. doi request reprint Identification of the molecular composition of the 20S proteasome of mouse intestine by high-resolution mass spectrometric proteome analysis
    Reinhold Weber
    Laboratory of Analytical Chemistry and Biopolymer Structure Analysis, Department of Chemistry, University of Konstanz, Konstanz, Germany
    Methods Mol Biol 564:173-86. 2009
    ..In addition, the comparison of UV- and IR-MALDI FTICR-MS may provide details of fragmentation and rearrangement reactions that may occur under UV-MALDI ionisation conditions...
  28. doi request reprint Activating the ubiquitin family: UBA6 challenges the field
    Marcus Groettrup
    Division of Immunology, Department of Biology, University of Constance, D 78457 Konstanz, Germany
    Trends Biochem Sci 33:230-7. 2008
    ..This new layer of regulation in ubiquitin activation markedly increases the versatility of the ubiquitin conjugation system...
  29. doi request reprint Microencapsulation of inorganic nanocrystals into PLGA microsphere vaccines enables their intracellular localization in dendritic cells by electron and fluorescence microscopy
    Christopher Schliehe
    Division of Immunology, Department of Biology, Constance University, 78457 Konstanz, Germany
    J Control Release 151:278-85. 2011
    ..Our data argue against an escape of PLGA-MS from the endosome as has previously been suggested as a mechanism to facilitate cross-presentation of PLGA-MS encapsulated antigen...
  30. ncbi request reprint The unique functions of tissue-specific proteasomes
    Andrea Kniepert
    Division of Immunology, Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    Trends Biochem Sci 39:17-24. 2014
    ..Here, we outline how tissue-specific proteasomes adapt to functional needs in their respective tissues and how their selective inhibition may be used to interfere with autoimmune diseases and cancer. ..
  31. doi request reprint FAT10ylation as a signal for proteasomal degradation
    Gunter Schmidtke
    Division of Immunology, Department of Biology, University of Konstanz, D 78457 Konstanz, Germany
    Biochim Biophys Acta 1843:97-102. 2014
    ..This article is part of a Special Issue entitled: Ubiquitin-Proteasome System. Guest Editors: Thomas Sommer and Dieter H. Wolf. ..
  32. ncbi request reprint Immunoproteasomes down-regulate presentation of a subdominant T cell epitope from lymphocytic choriomeningitis virus
    Michael Basler
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    J Immunol 173:3925-34. 2004
    ..Taken together, we show that LMP2 and LMP7 can at least in part determine subdominance and shape the epitope hierarchy of CTL responses in vivo...
  33. doi request reprint Substantial reduction of naïve and regulatory T cells following traumatic stress
    Annette Sommershof
    Division of Immunology, University of Konstanz, Konstanz, Germany
    Brain Behav Immun 23:1117-24. 2009
    ....
  34. doi request reprint The proteasome inhibitor bortezomib enhances the susceptibility to viral infection
    Michael Basler
    Biotechnology Institute Thurgau BITg at Constance University, CH 8280 Kreuzlingen, Switzerland
    J Immunol 183:6145-50. 2009
    ..In summary, we show that proteasome inhibition with bortezomib led to an increased susceptibility to viral infection, and demonstrate for the first time, that proteasome inhibitors can alter Ag processing in vivo...
  35. doi request reprint Distinct motifs in the chemokine receptor CCR7 regulate signal transduction, receptor trafficking and chemotaxis
    Carolina Otero
    Department of Biology, Division of Immunology, University of Konstanz, Konstanz, Germany
    J Cell Sci 121:2759-67. 2008
    ..Finally, G-protein coupling was critical for [Ca2+](i) mobilization, Erk1/2 phosphorylation and chemotaxis, but not for CCR7 trafficking...
  36. ncbi request reprint Quantitative analysis of gene expression relative to 18S rRNA in carcinoma samples using the LightCycler instrument and a SYBR GreenI-based assay: determining FAT10 mRNA levels in hepatocellular carcinoma
    Sebastian Lukasiak
    Department of Biology, University of Konstanz Universitätsstr, Konstanz, Germany
    Methods Mol Biol 429:59-72. 2008
    ..This method can be easily adapted to any tissue (human or mouse, rat, etc.) and any gene...
  37. ncbi request reprint UBE1L2, a novel E1 enzyme specific for ubiquitin
    Christiane Pelzer
    Division of Immunology, Department of Biology, University of Constance, 78457 Konstanz, Germany
    J Biol Chem 282:23010-4. 2007
    ..The UBE1L2 mRNA is most abundantly expressed in the testis, suggesting an organ-specific regulation of ubiquitin activation...
  38. ncbi request reprint The UBA domains of NUB1L are required for binding but not for accelerated degradation of the ubiquitin-like modifier FAT10
    Gunter Schmidtke
    Division of Immunology, Department of Biology, University of Konstanz, Universitaetsstr 10, D 78457 Konstanz, Germany
    J Biol Chem 281:20045-54. 2006
    ..We propose that NUB1L not only acts as a linker between the 26 S proteasome and ubiquitin-like proteins, but also as a facilitator of proteasomal degradation...
  39. doi request reprint The combination of TLR-9 adjuvantation and electroporation-mediated delivery enhances in vivo antitumor responses after vaccination with HPV-16 E7 encoding DNA
    Peter Öhlschläger
    Department of Immunology, University of Constance, 78457 Constance, Germany
    Int J Cancer 128:473-81. 2011
    ..Collectively, these results provide a basis for the transfer of preclinical therapeutic DNA-based immunization studies into successful clinical cancer trials...
  40. ncbi request reprint Advances in prostate cancer immunotherapies
    Michael Basler
    Division of Immunology, Department of Biology, University of Constance, Konstanz, Germany
    Drugs Aging 24:197-221. 2007
    ..Although there is a minor increase in overall survival of metastatic prostate cancer patients with some approaches, more effective therapeutic strategies need to be developed...
  41. ncbi request reprint NEDD8 ultimate buster-1L interacts with the ubiquitin-like protein FAT10 and accelerates its degradation
    Mark Steffen Hipp
    Department of Biology, Division of Immunology, University of Constance, Universitatsstrasse 10, D 78457 Konstanz, Germany
    J Biol Chem 279:16503-10. 2004
    ..Because NUB1 was shown to bind to the proteasome subunit RPN10 in vitro and to be contained in 26 S proteasome preparations, it may function as a linker that targets FAT10 for degradation by the proteasome...