Joachim Griesenbeck

Summary

Affiliation: University of Regensburg
Country: Germany

Publications

  1. pmc Local tertiary structure probing of ribonucleoprotein particles by nuclease fusion proteins
    Uli Ohmayer
    Lehrstuhl für Biochemie III, Universitat Regensburg, Regensburg, Germany
    PLoS ONE 7:e42449. 2012
  2. pmc Interrelationships between yeast ribosomal protein assembly events and transient ribosome biogenesis factors interactions in early pre-ribosomes
    Steffen Jakob
    Lehrstuhl für Biochemie III, Universitat Regensburg, Regensburg, Germany
    PLoS ONE 7:e32552. 2012
  3. pmc Alternative chromatin structures of the 35S rRNA genes in Saccharomyces cerevisiae provide a molecular basis for the selective recruitment of RNA polymerases I and II
    Hannah Goetze
    Universitätsstr 31, D 93053 Regensburg, Germany
    Mol Cell Biol 30:2028-45. 2010
  4. pmc Reduction in ribosomal protein synthesis is sufficient to explain major effects on ribosome production after short-term TOR inactivation in Saccharomyces cerevisiae
    Alarich Reiter
    Universitaet Regensburg, Institut fur Biochemie, Genetik und Mikrobiologie, Lehrstuhl Biochemie III, Regensburg, Germany
    Mol Cell Biol 31:803-17. 2011
  5. pmc Compositional and structural analysis of selected chromosomal domains from Saccharomyces cerevisiae
    Stephan Hamperl
    Universitat Regensburg, Biochemie Zentrum Regensburg BZR, Lehrstuhl für Biochemie III, 93053 Regensburg, Germany and Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    Nucleic Acids Res 42:e2. 2014
  6. doi request reprint RNA polymerase I termination: Where is the end?
    Attila Nemeth
    Universitat Regensburg, Biochemie Zentrum Regensburg BZR, Lehrstuhl Biochemie III, 93053 Regensburg, Germany
    Biochim Biophys Acta 1829:306-17. 2013
  7. pmc Studies on the assembly characteristics of large subunit ribosomal proteins in S. cerevisae
    Uli Ohmayer
    Lehrstuhl für Biochemie III, Universitat Regensburg, Regensburg, Germany
    PLoS ONE 8:e68412. 2013
  8. ncbi request reprint Purification of specific chromatin domains from single-copy gene loci in Saccharomyces cerevisiae
    Stephan Hamperl
    Biochemie Zentrum Regensburg BZR, Lehrstuhl Biochemie III, Universitat Regensburg, Regensburg, Germany
    Methods Mol Biol 1094:329-41. 2014
  9. pmc TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production
    Anja Philippi
    Institut fur Biochemie, Genetik und Mikrobiologie, Universitat Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Nucleic Acids Res 38:5315-26. 2010
  10. doi request reprint Establishment and maintenance of alternative chromatin states at a multicopy gene locus
    Manuel Wittner
    Lehrstuhl Biochemie III, Universitat Regensburg, Institut fur Biochemie, Genetik und Mikrobiologie, 93053 Regensburg, Germany
    Cell 145:543-54. 2011

Collaborators

  • Herbert Tschochner
  • Antonio Conconi
  • Attila Nemeth
  • Ed Hurt
  • Philipp Milkereit
  • Stephan Hamperl
  • Jorge Pérez-Fernández
  • Manuel Wittner
  • Uli Ohmayer
  • Alarich Reiter
  • Thomas Hierlmeier
  • Jochen Gerber
  • Virginia Babl
  • Rainer Deutzmann
  • Ulrike Stoeckl
  • Steffen Jakob
  • Anja Philippi
  • Robert Steinbauer
  • Hannah Goetze
  • Katharina Merz
  • Katharina Huber
  • Christopher R Brown
  • Hinrich Boeger
  • Maxime Tremblay
  • Astrid Bruckmann
  • Martina Sauert
  • Gisela Pöll
  • Ulrike Stöckl
  • Lydia Williams
  • Isabelle Léger-Silvestre
  • Maria Hondele
  • Ana Villar Garea
  • Romain Charton
  • Genevieve Levasseur
  • Patrick Schultz
  • Kristin Hergert
  • Jochen Baßler
  • Anja Jacob
  • Michael Gamalinda
  • Julius Ossowski
  • Juliane Merl
  • Beril Kumcuoglu
  • John Woolford
  • Marlène Faubladier
  • Reinhard Rachel
  • Jan Linnemann
  • Hannah Seitz
  • Isabelle Léger
  • Andreas Neueder
  • Olivier Gadal
  • Eduard Hochmuth
  • Philipp Merkl
  • Wolfgang Seufert
  • Stephan Fath
  • Katharina Gmelch
  • Claus Dieter Kuhn
  • Patrick Cramer

Detail Information

Publications17

  1. pmc Local tertiary structure probing of ribonucleoprotein particles by nuclease fusion proteins
    Uli Ohmayer
    Lehrstuhl für Biochemie III, Universitat Regensburg, Regensburg, Germany
    PLoS ONE 7:e42449. 2012
    ..Advantages and disadvantages of the use of MNase fusion proteins for local tertiary structure probing of RNPs as well as alternative applications for this type of approach in RNP research are discussed...
  2. pmc Interrelationships between yeast ribosomal protein assembly events and transient ribosome biogenesis factors interactions in early pre-ribosomes
    Steffen Jakob
    Lehrstuhl für Biochemie III, Universitat Regensburg, Regensburg, Germany
    PLoS ONE 7:e32552. 2012
    ..They point towards some of these components being transient primary pre-rRNA in vivo binders and towards a role for others in coordinating the assembly of major SSU domains...
  3. pmc Alternative chromatin structures of the 35S rRNA genes in Saccharomyces cerevisiae provide a molecular basis for the selective recruitment of RNA polymerases I and II
    Hannah Goetze
    Universitätsstr 31, D 93053 Regensburg, Germany
    Mol Cell Biol 30:2028-45. 2010
    ..Furthermore, lack of UAF leads to the loss of Sir2 from rDNA, correlating with impaired Pol II silencing. This analysis of rDNA chromatin provides a molecular basis, explaining many phenotypes observed in previous genetic analyses...
  4. pmc Reduction in ribosomal protein synthesis is sufficient to explain major effects on ribosome production after short-term TOR inactivation in Saccharomyces cerevisiae
    Alarich Reiter
    Universitaet Regensburg, Institut fur Biochemie, Genetik und Mikrobiologie, Lehrstuhl Biochemie III, Regensburg, Germany
    Mol Cell Biol 31:803-17. 2011
    ..We suggest that shortage of newly synthesized r-proteins after short-term TOR inactivation is sufficient to explain most of the observed effects on ribosome production...
  5. pmc Compositional and structural analysis of selected chromosomal domains from Saccharomyces cerevisiae
    Stephan Hamperl
    Universitat Regensburg, Biochemie Zentrum Regensburg BZR, Lehrstuhl für Biochemie III, 93053 Regensburg, Germany and Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    Nucleic Acids Res 42:e2. 2014
    ..We also provide evidence that chromatin from virtually every single copy genomic locus of interest can be purified and analyzed by this technique. ..
  6. doi request reprint RNA polymerase I termination: Where is the end?
    Attila Nemeth
    Universitat Regensburg, Biochemie Zentrum Regensburg BZR, Lehrstuhl Biochemie III, 93053 Regensburg, Germany
    Biochim Biophys Acta 1829:306-17. 2013
    ..This article is part of a Special Issue entitled: Transcription by Odd Pols...
  7. pmc Studies on the assembly characteristics of large subunit ribosomal proteins in S. cerevisae
    Uli Ohmayer
    Lehrstuhl für Biochemie III, Universitat Regensburg, Regensburg, Germany
    PLoS ONE 8:e68412. 2013
    ..Effects of these proteins on the assembly states of other r-proteins and on the transient LSU precursor association of several ribosome biogenesis factors, including Nog2, Rsa4 and Nop53, are discussed. ..
  8. ncbi request reprint Purification of specific chromatin domains from single-copy gene loci in Saccharomyces cerevisiae
    Stephan Hamperl
    Biochemie Zentrum Regensburg BZR, Lehrstuhl Biochemie III, Universitat Regensburg, Regensburg, Germany
    Methods Mol Biol 1094:329-41. 2014
    ..This technique appears to be suitable to obtain a detailed description of chromatin composition and specific posttranslational histone modification state at virtually any genomic locus in yeast. ..
  9. pmc TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production
    Anja Philippi
    Institut fur Biochemie, Genetik und Mikrobiologie, Universitat Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Nucleic Acids Res 38:5315-26. 2010
    ..However, our analysis reveals that the dramatic reduction of rRNA synthesis in the immediate cellular response to impaired TOR signalling cannot be explained by the simple down-regulation of Rrn3p and Pol I-Rrn3p levels...
  10. doi request reprint Establishment and maintenance of alternative chromatin states at a multicopy gene locus
    Manuel Wittner
    Lehrstuhl Biochemie III, Universitat Regensburg, Institut fur Biochemie, Genetik und Mikrobiologie, 93053 Regensburg, Germany
    Cell 145:543-54. 2011
    ..The findings indicate that the opposing effects of replication and transcription lead to a de novo establishment of chromatin states for rRNA genes during each cell cycle...
  11. pmc UV light-induced DNA lesions cause dissociation of yeast RNA polymerases-I and establishment of a specialized chromatin structure at rRNA genes
    Maxime Tremblay
    Departement De Microbiologie et Infectiologie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, QC J1E 4K8, Canada and Institut für Biochemie, Genetik und Mikrobiologie, Universitat Regensburg, 93053 Regensburg, Germany
    Nucleic Acids Res 42:380-95. 2014
    ..After repair of DNA lesions, Hmo1 containing chromatin might help to restore transcription elongation and reopening of rRNA genes chromatin. ..
  12. pmc The Reb1-homologue Ydr026c/Nsi1 is required for efficient RNA polymerase I termination in yeast
    Alarich Reiter
    Universitat Regensburg, Biochemie Zentrum Regensburg BZR, Lehrstuhl Biochemie III, Regensburg, Germany
    EMBO J 31:3480-93. 2012
    ..Possible Nsi1 functions in regard to the mechanism of transcription termination are discussed...
  13. doi request reprint Chromatin states at ribosomal DNA loci
    Stephan Hamperl
    Lehrstuhl Biochemie III, Universitat Regensburg, 93053 Regensburg, Germany
    Biochim Biophys Acta 1829:405-17. 2013
    ..The establishment and maintenance of rRNA gene chromatin states and their impact on rRNA synthesis are discussed. This article is part of a Special Issue entitled: Transcription by Odd Pols...
  14. pmc Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae
    Thomas Hierlmeier
    Universitat Regensburg, Biochemie Zentrum Regensburg BZR, Lehrstuhl Biochemie III, 93053 Regensburg, Germany
    Nucleic Acids Res 41:1191-210. 2013
    ..Taken together, our results suggest a co- and post-transcriptional role of the yeast Rrp5p-Noc1p-Noc2p module in the structural organization of early LSU precursors protecting them from non-productive RNase activity...
  15. doi request reprint Chromatin endogenous cleavage and psoralen crosslinking assays to analyze rRNA gene chromatin in vivo
    Joachim Griesenbeck
    Naturwissenschaftliche Fakultät III, Institut für Biochemie III, Universitat Regensburg, Regensburg, Germany
    Methods Mol Biol 809:291-301. 2012
    ..Here, we provide a protocol to investigate the specific association of proteins with the two rRNA gene chromatin populations in vivo, using Saccharomyces cerevisiae as a model eukaryote...
  16. pmc Site specific phosphorylation of yeast RNA polymerase I
    Jochen Gerber
    Institut fur Biochemie, Mikrobiologie und Genetik, Universitat Regensburg, Munich, Germany
    Nucleic Acids Res 36:793-802. 2008
    ..2, suggesting a role in RNA cleavage/elongation or termination. Our results suggest that individual major or constitutively phosphorylated residues contribute to non-essential Pol I-functions...
  17. pmc Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules
    Katharina Merz
    Universitaet Regensburg, Institut fur Biochemie, Genetik und Mikrobiologie, 93053 Regensburg, Germany
    Genes Dev 22:1190-204. 2008
    ..We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1...