Andreus Greinacher

Summary

Affiliation: University of Greifswald
Country: Germany

Publications

  1. ncbi Heparin-induced thrombocytopenia: frequency and pathogenesis
    Andreas Greinacher
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Pathophysiol Haemost Thromb 35:37-45. 2006
  2. ncbi Hirudin in heparin-induced thrombocytopenia
    Norbert Lubenow
    Ernst Moritz Arndt University, Greifswald, Germany
    Semin Thromb Hemost 28:431-8. 2002
  3. ncbi Gender imbalance and risk factor interactions in heparin-induced thrombocytopenia
    Theodore E Warkentin
    Department of Pathology, Hamilton Regional Laboratory Medicine Program, Rm 1 180A, Hamilton Health Sciences, Hamilton General Site, McMaster University, 237 Barton St E, Hamilton, ON L8L2X2 Canada
    Blood 108:2937-41. 2006
  4. ncbi The use of direct thrombin inhibitors in cardiovascular surgery in patients with heparin-induced thrombocytopenia
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Diagnostikzentrum, Greifswald, Germany
    Semin Thromb Hemost 30:315-27. 2004
  5. doi [Autoimmune thrombocytopenia, neutropenia and hemolysis]
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Universitatsklinikum, Ernst Moritz Arndt Universitat Greifswald, Sauerbruchstrasse, 17487, Greifswald, Deutschland
    Internist (Berl) 50:276-90. 2009
  6. ncbi Lepirudin: a bivalent direct thrombin inhibitor for anticoagulation therapy
    Andreas Greinacher
    Ernst Moritz Arndt Universitat, Institut für Immunologie und Transfusionmedizin, Klinikum Sauerbruchstrasse, 17489 Greifswald, Germany
    Expert Rev Cardiovasc Ther 2:339-57. 2004
  7. doi The temporal profile of the anti-PF4/heparin immune response
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Germany
    Blood 113:4970-6. 2009
  8. doi The direct thrombin inhibitor hirudin
    Andreas Greinacher
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Sauerbruchstrasse, 17489 Greifswald, Germany
    Thromb Haemost 99:819-29. 2008
  9. doi Heparin-induced thrombocytopenia: a stoichiometry-based model to explain the differing immunogenicities of unfractionated heparin, low-molecular-weight heparin, and fondaparinux in different clinical settings
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Greifswald Germany
    Thromb Res 122:211-20. 2008
  10. ncbi Heparin-induced thrombocytopenia: a prospective study on the incidence, platelet-activating capacity and clinical significance of antiplatelet factor 4/heparin antibodies of the IgG, IgM, and IgA classes
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin Ernst Moritz Arndt Universität Greifswald, Greifswald, Germany
    J Thromb Haemost 5:1666-73. 2007

Collaborators

Detail Information

Publications86

  1. ncbi Heparin-induced thrombocytopenia: frequency and pathogenesis
    Andreas Greinacher
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Pathophysiol Haemost Thromb 35:37-45. 2006
    ..This review summarizes data on pathogenesis and frequencies of HIT in different patient population...
  2. ncbi Hirudin in heparin-induced thrombocytopenia
    Norbert Lubenow
    Ernst Moritz Arndt University, Greifswald, Germany
    Semin Thromb Hemost 28:431-8. 2002
    ..The choice between the available drugs for HIT, namely lepirudin, danaparoid, and argatroban, has to be made according to the clinical presentation of the patient...
  3. ncbi Gender imbalance and risk factor interactions in heparin-induced thrombocytopenia
    Theodore E Warkentin
    Department of Pathology, Hamilton Regional Laboratory Medicine Program, Rm 1 180A, Hamilton Health Sciences, Hamilton General Site, McMaster University, 237 Barton St E, Hamilton, ON L8L2X2 Canada
    Blood 108:2937-41. 2006
    ..93 vs 1.75; P = .005). We conclude that females are at greater risk for HIT and that using LMWH to prevent HIT may have greatest absolute benefit in females undergoing surgical thromboprophylaxis...
  4. ncbi The use of direct thrombin inhibitors in cardiovascular surgery in patients with heparin-induced thrombocytopenia
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Diagnostikzentrum, Greifswald, Germany
    Semin Thromb Hemost 30:315-27. 2004
    ..Before and after surgery, however, alternative anticoagulants should be used. The risk of clinical HIT after heart surgery could potentially be reduced by using low-molecular-weight heparins for postsurgery anticoagulation...
  5. doi [Autoimmune thrombocytopenia, neutropenia and hemolysis]
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Universitatsklinikum, Ernst Moritz Arndt Universitat Greifswald, Sauerbruchstrasse, 17487, Greifswald, Deutschland
    Internist (Berl) 50:276-90. 2009
    ..Growth factors like the new thrombopoietin receptor agnonists in autoimmune thrombocytopenia or G-CSF in autoimmune neutropenia, and anti-CD20 antibodies are new options for treatment...
  6. ncbi Lepirudin: a bivalent direct thrombin inhibitor for anticoagulation therapy
    Andreas Greinacher
    Ernst Moritz Arndt Universitat, Institut für Immunologie und Transfusionmedizin, Klinikum Sauerbruchstrasse, 17489 Greifswald, Germany
    Expert Rev Cardiovasc Ther 2:339-57. 2004
    ..The review concludes with a discussion of pharmacokinetic and clinical data supporting the potential for subcutaneous administration of lepirudin...
  7. doi The temporal profile of the anti-PF4/heparin immune response
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Germany
    Blood 113:4970-6. 2009
    ....
  8. doi The direct thrombin inhibitor hirudin
    Andreas Greinacher
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Sauerbruchstrasse, 17489 Greifswald, Germany
    Thromb Haemost 99:819-29. 2008
    ..Drawbacks of hirudin and important precautions for avoiding potential adverse effects are discussed in detail in the third part of this review...
  9. doi Heparin-induced thrombocytopenia: a stoichiometry-based model to explain the differing immunogenicities of unfractionated heparin, low-molecular-weight heparin, and fondaparinux in different clinical settings
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Greifswald Germany
    Thromb Res 122:211-20. 2008
    ..We aimed to provide a model that included anticoagulant concentrations and PF4 availability as risk factors influencing the anti-PF4/heparin immune response...
  10. ncbi Heparin-induced thrombocytopenia: a prospective study on the incidence, platelet-activating capacity and clinical significance of antiplatelet factor 4/heparin antibodies of the IgG, IgM, and IgA classes
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin Ernst Moritz Arndt Universität Greifswald, Greifswald, Germany
    J Thromb Haemost 5:1666-73. 2007
    ..However, the relative utility of functional (platelet activation) vs. antigen [enzyme-immunoassay (EIA)] assays, and the significance of assay discrepancies remain unresolved...
  11. ncbi [Thrombosis prophylaxis with heparins in intensive care patients]
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin der Ernst Moritz Arndt Universität Greifswald
    Anasthesiol Intensivmed Notfallmed Schmerzther 40:156-63. 2005
    ..Precautions such as monitoring of anticoagulation in patients with renal insufficiency are fundamental if LMWH is given. Further investigations into prevention of venous thromboembolism in ICU patients are urgently needed...
  12. ncbi [Recommendations for platelet transfusion by the Joint Thrombocyte Working Party of the German Societies of Transfusion Medicine and Immunohaematology (DGTI), Thrombosis and Haemostasis Research (GTH), and Haematology and Oncology (DGHO)]
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald
    Dtsch Med Wochenschr 131:2675-9. 2006
  13. ncbi Close approximation of two platelet factor 4 tetramers by charge neutralization forms the antigens recognized by HIT antibodies
    Andreas Greinacher
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Sauerbruchstrasse, 17489 Greifswald, Germany
    Arterioscler Thromb Vasc Biol 26:2386-93. 2006
    ..To better understand these findings, we analyzed the molecular structure of the complexes formed between PF4 and UFH, LMWH, or fondaparinux...
  14. ncbi Clinical features of heparin-induced thrombocytopenia including risk factors for thrombosis. A retrospective analysis of 408 patients
    Andreas Greinacher
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Sauerbruchstr Diagnostikzentrum, 17487 Greifswald, Germany
    Thromb Haemost 94:132-5. 2005
    ..HIT-associated thrombosis occurs in a considerable proportion of patients before platelet counts decrease by more than 50%...
  15. ncbi Recognition, treatment, and prevention of heparin-induced thrombocytopenia: review and update
    Andreas Greinacher
    Ernst Moritz Arndt Univerität Greifswald, Institut für Immunologie und Transfusionsmedizin, Germany
    Thromb Res 118:165-76. 2006
    ....
  16. doi Megakaryocyte impairment by eptifibatide-induced antibodies causes prolonged thrombocytopenia
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Blood 114:1250-3. 2009
    ..These findings can be explained by impaired megakaryocytopoiesis complicating anti-GPIIbIIIa antibody-mediated immune thrombocytopenia. This mechanism may also apply to some patients with autoimmune thrombocytopenia...
  17. doi Heparin-induced thrombocytopenia
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Sauerbruchstr, Greifswald, Germany
    J Thromb Haemost 7:9-12. 2009
    ..Recent studies of HIT immunobiology suggest that HIT mimics immunity against repetitive antigens, as they are relevant in microbial defense. Thus, understanding HIT may help unravel why host defenses can trigger autoimmunity...
  18. ncbi Treatment options for heparin-induced thrombocytopenia
    Andreas Greinacher
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Diagnostikzentrum Saverbruchstrasse, 17487 Greifswald, Germany
    Am J Health Syst Pharm 60:S12-8. 2003
    ....
  19. doi Association of natural anti-platelet factor 4/heparin antibodies with periodontal disease
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Blood 118:1395-401. 2011
    ..001). Thus, preimmunization to PF4/bacteria complexes by prevalent infections, for example, periodontitis, likely explains the presence of natural anti-PF4/heparin Abs and the early occurrence of anti-PF4/H-IgG in HIT...
  20. doi Thrombocytopenia in the intensive care unit patient
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Universitatsklinikum, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    Hematology Am Soc Hematol Educ Program 2010:135-43. 2010
    ....
  21. doi Risk of heparin-induced thrombocytopenia in patients receiving thromboprophylaxis
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Sauerbruchstraße, 17487 Greifswald, Germany
    Expert Rev Hematol 1:75-85. 2008
    ..Treatment of HIT involves heparin cessation together with anticoagulation with direct thrombin inhibitors or indirect factor Xa inhibitors...
  22. doi Heparin-induced thrombocytopenia: towards standardization of platelet factor 4/heparin antigen tests
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    J Thromb Haemost 8:2025-31. 2010
    ..Platelet factor 4 (PF4)/heparin enzyme-immunoassays (EIA) are a widely available surrogate for platelet-activating antibodies...
  23. ncbi Antihirudin antibodies following low-dose subcutaneous treatment with desirudin for thrombosis prophylaxis after hip-replacement surgery: incidence and clinical relevance
    Andreas Greinacher
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Blood 101:2617-9. 2003
    ..However, the total number of immunized patients observed was low and so infrequent (but severe) effects of antihirudin antibodies cannot be excluded...
  24. ncbi Impact of demographic changes on the blood supply: Mecklenburg-West Pomerania as a model region for Europe
    Andreus Greinacher
    Institute for Immunology and Transfusion Medicine, Department of Epidemiology of Health Care and Community Health, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
    Transfusion 47:395-401. 2007
    ..The population structure in most European countries is currently changing with a shift from younger to older age groups. This study analyzed how demography will determine future blood demand and supply in a well-characterized region...
  25. ncbi Heparin-induced thrombocytopenia
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald
    Hamostaseologie 30:17-8, 20-8. 2010
    ..Recent studies of HIT immunobiology suggest that HIT mimics immunity against repetitive antigens, as are relevant in microbial defense. Thus, understanding HIT may help unravel why host defenses can trigger autoimmunity...
  26. doi Characterization of the human neutrophil alloantigen-3a
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    Nat Med 16:45-8. 2010
    ..The molecular identification of this antigen should facilitate the development of assays for blood donor screening to lower the risk of TRALI...
  27. ncbi Anaphylactic and anaphylactoid reactions associated with lepirudin in patients with heparin-induced thrombocytopenia
    A Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Klinikum Sauerbruchstrasse, 17489 Greifswald, Germany
    Circulation 108:2062-5. 2003
    ..Because 3 cases of fatal anaphylaxis possibly associated with use of lepirudin have been reported, we initiated an investigation of putative lepirudin-associated anaphylaxis...
  28. doi Treatment of severe neurological deficits with IgG depletion through immunoadsorption in patients with Escherichia coli O104:H4-associated haemolytic uraemic syndrome: a prospective trial
    Andreas Greinacher
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Lancet 378:1166-73. 2011
    ..Some patients needed ventilatory support due to severe neurological complications, which arose 1 week after onset of enteritis, suggesting an antibody-mediated mechanism. Therefore, we aimed to assess immunoadsorption as rescue therapy...
  29. ncbi Incidence and clinical significance of anti-PF4/heparin antibodies of the IgG, IgM, and IgA class in 755 consecutive patient samples referred for diagnostic testing for heparin-induced thrombocytopenia
    David Juhl
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Eur J Haematol 76:420-6. 2006
    ..While antigen assays are very sensitive and recognize immunoglobulin (Ig)G, IgA, and IgM antibodies, the role of IgM and IgA HIT-antibodies is debated. Platelet activation assays recognize IgG and are more specific for clinical HIT...
  30. ncbi Heparin-induced thrombocytopenia in intensive care patients
    Kathleen Selleng
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Crit Care Med 35:1165-76. 2007
    ....
  31. doi Management of anticoagulation in patients with subacute heparin-induced thrombocytopenia scheduled for heart transplantation
    Sixten Selleng
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Blood 112:4024-7. 2008
    ..This can be lifesaving in patients with end-stage heart failure...
  32. doi The severity of trauma determines the immune response to PF4/heparin and the frequency of heparin-induced thrombocytopenia
    Norbert Lubenow
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald
    Blood 115:1797-803. 2010
    ..In major trauma certoparin may be safer than UFH because it induces HIT-antibody seroconversion, and the corresponding risk of HIT, less frequently...
  33. doi Studies of the anti-platelet factor 4/heparin immune response: adapting the enzyme-linked immunosorbent spot assay for detection of memory B cells against complex antigens
    Kathleen Selleng
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Transfusion 50:32-9. 2010
    ....
  34. ncbi The new ID-heparin/PF4 antibody test for rapid detection of heparin-induced antibodies in comparison with functional and antigenic assays
    Petra Eichler
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Br J Haematol 116:887-91. 2002
    ..Evaluation of the test in a prospective setting as well as interlaboratory variation should be assessed as a next step...
  35. doi Heparin-induced thrombocytopenia in intensive care patients
    Kathleen Selleng
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Semin Thromb Hemost 34:425-38. 2008
    ..Therefore, accurate diagnosis of HIT and individual choice of alternative anticoagulant are important for the adequate management of critically ill HIT patients...
  36. ncbi Benefit and risk of heparin for maintaining peripheral venous catheters in neonates: a placebo-controlled trial
    Anne Friederike Klenner
    Department of Pediatrics, the Institute of Immunology and Transfusion Medicine, and the University Pharmacy, Ernst Moritz Arndt University, Greifswald, Germany
    J Pediatr 143:741-5. 2003
    ..One objective was peripheral venous catheter patency with heparinization of continuous infusions (0.5 IU/mL). Secondary objectives were incidences of bleeding, clinically manifest HIT, HIT antibodies, and catheter-related complications...
  37. pmc Role of MRP4 (ABCC4) in platelet adenine nucleotide-storage: evidence from patients with delta-storage pool deficiencies
    Gabriele Jedlitschky
    Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Institut fur Pharmakologie, Ernst Moritz Arndt University, Friedrich Loeffler Str 23d, D 17487, Greifswald, Germany
    Am J Pathol 176:1097-103. 2010
    ..Thus, defective expression of platelet MRP4 is associated with selective defect in AN storage. The genetic basis of the new delta-SPD phenotype remains to be elucidated...
  38. ncbi Heparin-induced thrombocytopenia: temporal pattern of thrombocytopenia in relation to initial use or reexposure to heparin
    Norbert Lubenow
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Chest 122:37-42. 2002
    ..We therefore analyzed the temporal pattern of thrombocytopenia in patients with laboratory-confirmed HIT to assess whether patients with previous heparin exposure have an increased risk of early onset of HIT...
  39. doi Incidence and clinical relevance of anti-platelet factor 4/heparin antibodies before cardiac surgery
    Sixten Selleng
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Am Heart J 160:362-9. 2010
    ..Whether preoperative presence of antibodies confers adverse prognosis and which particular antibody classes (IgG, IgA, IgM) might be implicated are unknown...
  40. ncbi Should patients be informed about the risk of heparin-induced thrombocytopenia before prolonged low-molecular-weight heparin thromboprophylaxis post-trauma/orthopedic surgery?
    Norbert Lubenow
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Eur J Haematol 79:187-90. 2007
    ..Paradoxically, refusal of thromboprophylaxis as a result of seeking informed consent could increase risk for thrombosis...
  41. ncbi Heparin-induced thrombocytopenia and cardiopulmonary bypass: perioperative argatroban use
    Norbert Lubenow
    Institute of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Ann Thorac Surg 75:577-9. 2003
    ..Because of interference with the international normalized ratio (INR), switching from argatroban to oral anticoagulants is not straightforward...
  42. doi Immune mechanisms in heparin-induced thrombocytopenia: no evidence for immunoglobulin M anti-idiotype antibodies
    Kathleen Selleng
    Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
    Transfusion 49:1812-8. 2009
    ..We hypothesized that these unusual features could be caused by loss of regulatory anti-idiotype IgM antibodies due to disturbance (e.g., by surgery) of an idiotype-anti-idiotype network...
  43. doi The diagnostic value of the anti-PF4/heparin immunoassay high-dose heparin confirmatory test in cardiac surgery patients
    Sixten Selleng
    Klinik und Poliklinik für Anästhesiologie und Intensivmedizin, Ernst Moritz Arndt Universitat Greifswald, Friedrich Loeffler Strasse 23b, 17475 Greifswald, Germany
    Anesth Analg 112:774-6. 2011
    ..Therefore, the high-dose heparin confirmatory test should be used with caution to exclude platelet-activating antiplatelet factor 4/heparin antibodies or clinical HIT...
  44. ncbi Heparin-induced thrombocytopenia in children: 12 new cases and review of the literature
    Anne F Klenner
    Ernst Moritz Arndt Universitat Greifswald, Institut für Immunologie und Transfusionsmedizin, Sauerbruchstr Diagnostikzentrum, 17487 Greifswald, Germany
    Thromb Haemost 91:719-24. 2004
    ..Most data are available for lepirudin and danaparoid. Substitution of unfractionated heparin by low-molecular-weight heparins for regular anticoagulation may reduce the incidence of HIT...
  45. ncbi Immune heparin-induced thrombocytopenia can occur in patients receiving clopidogrel and aspirin
    Kathleen Selleng
    Institute of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Am J Hematol 78:188-92. 2005
    ..We conclude that combined therapy with aspirin and clopidogrel does not necessarily protect against clinical HIT, at least in patients with HIT antibodies that have strong platelet-activating characteristics...
  46. ncbi Antibodies against lepirudin are polyspecific and recognize epitopes on bivalirudin
    Petra Eichler
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Sauerbruchstr Diagnostikzentrum, 17487 Greifswald, Germany
    Blood 103:613-6. 2004
    ..Yet, as lepirudin and antilepirudin antibodies have recently been implicated in severe anaphylactic reactions, caution is warranted when using bivalirudin in patients previously treated with lepirudin...
  47. ncbi Ceftriaxone causes drug-induced immune thrombocytopenia and hemolytic anemia: characterization of targets on platelets and red blood cells
    Beatrice Grossjohann
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Germany
    Transfusion 44:1033-40. 2004
    ..A clinical and serologic analysis of the first two patients with severe drug-induced thrombocytopenia (DITP) due to ceftriaxone and one patient with fatal DIHA is reported...
  48. ncbi Very severe thrombocytopenia and fragmentation hemolysis mimicking thrombotic thrombocytopenic purpura associated with a giant intracardiac vegetation infected with Staphylococcus epidermidis: role of monocyte procoagulant activity induced by bacterial su
    Kathleen Selleng
    Department of Transfusion Medicine and Immunology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Am J Hematol 82:766-71. 2007
    ....
  49. ncbi Platelet receptor and clotting factor polymorphisms as genetic risk factors for thromboembolic complications in heparin-induced thrombocytopenia
    Lena E Carlsson
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Pharmacogenetics 13:253-8. 2003
    ..However, this would need to be assessed in further larger studies. Most likely, the high levels of thrombin generation during acute HIT are so procoagulant that less pronounced risk factors such as polymorphisms are overshadowed...
  50. ncbi Results of a systematic evaluation of treatment outcomes for heparin-induced thrombocytopenia in patients receiving danaparoid, ancrod, and/or coumarin explain the rapid shift in clinical practice during the 1990s
    Norbert Lubenow
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Thromb Res 117:507-15. 2006
    ..During the 1990s, danaparoid+/-coumarin began to replace ancrod (+/-coumarin), or coumarin alone, for treating HIT, despite danaparoid not being approved for treatment of HIT...
  51. doi Human platelets express organic anion-transporting peptide 2B1, an uptake transporter for atorvastatin
    Juliane Niessen
    Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Friedrich Loeffler Str 23 D, D 17487 Greifswald, Germany
    Drug Metab Dispos 37:1129-37. 2009
    ..In conclusion, OATP2B1 is an uptake transporter expressed in platelets and is involved in statin-mediated alteration of platelet aggregation...
  52. doi Hematopoietic stem cell differentiation affects expression and function of MRP4 (ABCC4), a transport protein for signaling molecules and drugs
    Lena Oevermann
    Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Greifswald, Germany
    Int J Cancer 124:2303-11. 2009
    ....
  53. ncbi The nucleotide transporter MRP4 (ABCC4) is highly expressed in human platelets and present in dense granules, indicating a role in mediator storage
    Gabriele Jedlitschky
    Department of Pharmacology, Peter Holtz Research Center of Pharmacology and Experimental Therapeutics, Greifswald, Germany
    Blood 104:3603-10. 2004
    ..The results indicate a function of MRP4 in platelet mediator storage and inhibition of MRP4 may represent a novel mechanism for inhibition of platelet function by some anti-inflammatory drugs...
  54. ncbi Subfractionation and purification of intracellular granule-structures of human platelets: an improved method based on magnetic sorting
    Juliane Niessen
    Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Greifswald, Germany
    J Immunol Methods 328:89-96. 2007
    ..Therefore, studies requiring highly purified material (e.g. identification of specific transporters and receptors) will benefit from these results...
  55. ncbi Perioperative management of MYH9 hereditary macrothrombocytopenia (Fechtner syndrome)
    Kathleen Selleng
    Department of Transfusion Medicine and Immunology, Ernst Moritz Arndt University, Greifswald, Germany
    Eur J Haematol 79:263-8. 2007
    ..A major risk for these patients can be inappropriate treatment with long-term corticosteroids or splenectomy for misdiagnosed chronic autoimmune thrombocytopenia, as well as inadequate peri- and postoperative management...
  56. doi The adhesion and spreading of thrombocyte vesicles on electrode surfaces
    Víctor Agmo Hernández
    Institut fur Biochemie, Universitat Greifswald, Felix Hausdorff Str 4, D 17487, Greifswald, Germany
    Bioelectrochemistry 74:210-6. 2008
    ..The chronoamperometric measurements indicate the possibility of future applications to probe membrane properties of thrombocytes...
  57. ncbi Fc(gamma) receptors IIa on cardiomyocytes and their potential functional relevance in dilated cardiomyopathy
    Alexander Staudt
    Klinik für Innere Medizin B, Ernst Moritz Arndt Universitat, Greifswald, Germany
    J Am Coll Cardiol 49:1684-92. 2007
    ..The purpose of this study was to investigate how cardiac autoantibodies might contribute to cardiac dysfunction in patients suffering from dilated cardiomyopathy (DCM)...
  58. ncbi Proteomics of blood-based therapeutics: a promising tool for quality assurance in transfusion medicine
    Thomas Thiele
    Institute of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    BioDrugs 21:179-93. 2007
    ..Optimized preparation procedures and storage conditions will reduce the risk of protein alterations, which in turn may contribute to better recovery, reduced exposure to allogeneic proteins, and increased transfusion safety...
  59. ncbi Transfusion medicine in the era of genomics and proteomics
    Andreas Greinacher
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Transfus Med Rev 19:288-94. 2005
    ..By combining proteomics and immunohematology, those manufacturing processes least likely to generate posttranslational protein modifications will need to be identified...
  60. ncbi Profiling of alterations in platelet proteins during storage of platelet concentrates
    Thomas Thiele
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Transfusion 47:1221-33. 2007
    ..In contrast, proteomic technologies provide a comprehensive overview of the PLT proteome. High interassay variability, however, limits meaningful assessment of samples taken from the same product over time or before and after processing...
  61. doi Isolation of platelet granules
    Juliane Niessen
    Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Greifswald, Germany
    Curr Protoc Cell Biol . 2010
    ..The immunomagnetic sorting method is especially good for studies requiring highly purified material (e.g., for the identification of specific transporters and receptors)...
  62. doi Expression of ABC-type transport proteins in human platelets
    Juliane Niessen
    Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Greifswald, Germany
    Pharmacogenet Genomics 20:396-400. 2010
    ..Their functions may be related to glutathione and lipid homeostasis, secretion of lipid mediators, cell protection as well as drug transport...
  63. ncbi So, does low-molecular-weight heparin cause less heparin-induced thrombocytopenia than unfractionated heparin or not?
    Theodore E Warkentin
    Chest 132:1108-10. 2007
  64. ncbi Thrombosis of the cerebral veins and sinuses
    Theodore E Warkentin
    N Engl J Med 353:314-5. 2005
  65. ncbi Heparin-induced thrombocytopenia and cardiac surgery
    Theodore E Warkentin
    Department of Pathology, McMaster University, Hamilton, Ontario, Canada
    Ann Thorac Surg 76:2121-31. 2003
    ..As heparin-induced thrombocytopenia antibodies are transient, unfractionated heparin alone is appropriate in a patient with previous heparin-induced thrombocytopenia whose antibodies have disappeared...
  66. ncbi Lepirudin for prophylaxis of thrombosis in patients with acute isolated heparin-induced thrombocytopenia: an analysis of 3 prospective studies
    Norbert Lubenow
    Dept of Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Germany
    Blood 104:3072-7. 2004
    ..Dose reductions in renal impairment are important. Keeping the aPTT in the range corresponding to 600 to 700 microg/L lepirudin during treatment may minimize bleeding complications...
  67. doi Contaminated heparin
    Andreas Greinacher
    N Engl J Med 359:1291-2; author reply 1293. 2008
  68. ncbi Differences in the clinically effective molar concentrations of four direct thrombin inhibitors explain their variable prothrombin time prolongation
    Theodore E Warkentin
    Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
    Thromb Haemost 94:958-64. 2005
    ..DTIs with a relatively low affinity for thrombin require high plasma concentrations to double the APTT; these higher plasma concentrations, in turn, quench more of the thrombin generated in the PT, thereby more greatly prolonging the PT...
  69. ncbi Antigen-positive platelet transfusion in neonatal alloimmune thrombocytopenia (NAIT)
    Volker Kiefel
    Department of Transfusion Medicine, University of Rostock, Ernst Heydemann Strasse 6, D 18057 Rostock, Germany
    Blood 107:3761-3. 2006
    ..We conclude that transfusion of platelet concentrates from random donors is an appropriate strategy in the management of unexpected, severe NAIT predominantly in first pregnancies, pending the availability of compatible platelets...
  70. ncbi Replacement of unfractionated heparin by low-molecular-weight heparin for postorthopedic surgery antithrombotic prophylaxis lowers the overall risk of symptomatic thrombosis because of a lower frequency of heparin-induced thrombocytopenia
    Andreas Greinacher
    Blood 106:2921-2. 2005
  71. ncbi Heparin-induced thrombocytopenia in patients receiving mechanical circulatory support
    Soren Schenk
    Thoracic and Cardiovascular Surgery, Heart Center North Rhine Westphalia, Ruhr Universitat Bochum, Bad Oeynhausen, Germany
    J Thorac Cardiovasc Surg 131:1373-81.e4. 2006
    ..Patients receiving mechanical circulatory support are at risk for the development of heparin-induced thrombocytopenia due to the prolonged postoperative use of heparin. We evaluated their antibody status and outcome...
  72. pmc Anti-platelet factor 4/heparin antibodies in orthopedic surgery patients receiving antithrombotic prophylaxis with fondaparinux or enoxaparin
    Theodore E Warkentin
    Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada
    Blood 106:3791-6. 2005
    ..We conclude that despite similar immunogenicity of fondaparinux and LMWH, PF4/fondaparinux, but not PF4/LMWH, is recognized poorly by the antibodies generated, suggesting that the risk of HIT with fondaparinux likely is very low...
  73. ncbi Management of liver transplantation in a patient with a history of heparin-induced thrombocytopenia
    Reinhold Fretschner
    Department of Anesthesiology and Critical Care, University Hospital Tubingen, Tubingen, Germany
    Transpl Int 18:664-7. 2005
    ..Twenty months after an episode of HIT, a patient may receive an organ from a heparin-treated donor without risk of thrombocytopenia or thromboembolic complications. Avoidance of heparin for postoperative anticoagulation is recommended...
  74. ncbi Effect of fondaparinux on platelet activation in the presence of heparin-dependent antibodies: a blinded comparative multicenter study with unfractionated heparin
    Pierre Savi
    Cardiovascular Thrombosis Research Department, Sanofi Synthelabo Recherche, Toulouse, France
    Blood 105:139-44. 2005
    ..Together, these results suggest that fondaparinux is nonreactive to HIT sera and raise the possibility that the drug may be used for prophylaxis and treatment of thrombosis in patients with a history of HIT...
  75. doi HIT happens: diagnosing and evaluating the patient with heparin-induced thrombocytopenia
    Andreas Greinacher
    Anesth Analg 107:356-8. 2008
  76. ncbi Heparin-induced thrombocytopenia and cardiac surgery
    Theodore E Warkentin
    Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
    Ann Thorac Surg 76:638-48. 2003
    ..As heparin-induced thrombocytopenia antibodies are transient, unfractionated heparin alone is appropriate in a patient with previous heparin-induced thrombocytopenia whose antibodies have disappeared...
  77. ncbi Heparin-induced thrombocytopenia: recognition, treatment, and prevention: the Seventh ACCP Conference on Antithrombotic and Thrombolytic Therapy
    Theodore E Warkentin
    Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, General Site, 237 Barton St E, Hamilton, Ontario L8L 2X2, Canada
    Chest 126:311S-337S. 2004
    ....
  78. doi Bivalirudin
    Theodore E Warkentin
    Department of Pathology and Molecular Medicine, Michael G DeGroote School of Medicine, McMaster Unniversity, Hamilton, Ontario, L8L 2X2 Canada
    Thromb Haemost 99:830-9. 2008
    ....
  79. doi Treatment and prevention of heparin-induced thrombocytopenia: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines (8th Edition)
    Theodore E Warkentin
    Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, 237 Barton Street E, Hamilton, Ontario, Canada
    Chest 133:340S-380S. 2008
    ..For patients receiving VKAs at the time of diagnosis of HIT, we recommend use of vitamin K (10 mg po or 5 to 10 mg IV) [Grade 1C]...
  80. doi Postoperative complications after cardiac surgery and HIT: a word of caution
    Federico Pappalardo
    Ann Thorac Surg 86:1054-5; author reply 1055. 2008
  81. ncbi A variant of the Sebastian platelet syndrome with unique neutrophil inclusions
    James G White
    University of Minnesota School of Medicine, Menneapolis, MN 55455, USA
    Platelets 13:121-7. 2002
    ....
  82. ncbi Heparin-induced thrombocytopenia: a complication in extracorporeal photochemotherapy (photopheresis)
    Thomas Dittberner
    J Am Acad Dermatol 47:452-3. 2002
  83. ncbi Too many hits in HIT?
    Andreas Greinacher
    Am J Hematol 82:1035-6. 2007
  84. ncbi The risk of transfusion to the wrong patient in Germany
    Gregor Caspari
    Transfusion 42:1238-9. 2002
  85. ncbi A systematic review and survey of the management of unexpected neonatal alloimmune thrombocytopenia
    Dirk Bassler
    Department of Pediatrics, Neonatal Division, McMaster University, Hamilton, Ontario, Canada
    Transfusion 48:92-8. 2008
    ..Unexpected neonatal alloimmune thrombocytopenia (NAIT) may have devastating consequences and its management is challenging. To design future trials, evidence from the literature and existing best practice need review...
  86. ncbi Iron deficiency anaemia in young women
    Lena E Carlsson
    Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
    Eur J Haematol 68:341-4. 2002
    ..The aim of the study was to assess whether iron deficiency anaemia (e.g. increased blood loss) in young women could be associated with a specific genotype, indicating a role as potential evolutionary selection factor...