Research Topics
Species | Andreus GreinacherSummaryAffiliation: University of Greifswald Country: Germany Publications
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Publications
Heparin-induced thrombocytopenia: frequency and pathogenesisAndreas Greinacher
Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
Pathophysiol Haemost Thromb 35:37-45. 2006..This review summarizes data on pathogenesis and frequencies of HIT in different patient population...- Hirudin in heparin-induced thrombocytopeniaNorbert Lubenow
Ernst-Moritz-Arndt University, Greifswald, Germany
Semin Thromb Hemost 28:431-8. 2002..The choice between the available drugs for HIT, namely lepirudin, danaparoid, and argatroban, has to be made according to the clinical presentation of the patient... - Gender imbalance and risk factor interactions in heparin-induced thrombocytopeniaTheodore E Warkentin
Department of Pathology, Hamilton Regional Laboratory Medicine Program, Rm 1 180A, Hamilton Health Sciences, Hamilton General Site, McMaster University, 237 Barton St E, Hamilton, ON L8L2X2 Canada
Blood 108:2937-41. 2006..93 vs 1.75; P = .005). We conclude that females are at greater risk for HIT and that using LMWH to prevent HIT may have greatest absolute benefit in females undergoing surgical thromboprophylaxis... - The use of direct thrombin inhibitors in cardiovascular surgery in patients with heparin-induced thrombocytopeniaAndreas Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Diagnostikzentrum, Greifswald, Germany
Semin Thromb Hemost 30:315-27. 2004..Before and after surgery, however, alternative anticoagulants should be used. The risk of clinical HIT after heart surgery could potentially be reduced by using low-molecular-weight heparins for postsurgery anticoagulation... 
[Autoimmune thrombocytopenia, neutropenia and hemolysis]A Greinacher
Institut für Immunologie und Transfusionsmedizin, Universitatsklinikum, Ernst Moritz Arndt Universitat Greifswald, Sauerbruchstrasse, 17487, Greifswald, Deutschland
Internist (Berl) 50:276-90. 2009..Growth factors like the new thrombopoietin receptor agnonists in autoimmune thrombocytopenia or G-CSF in autoimmune neutropenia, and anti-CD20 antibodies are new options for treatment...- Lepirudin: a bivalent direct thrombin inhibitor for anticoagulation therapyAndreas Greinacher
Ernst Moritz Arndt Universitat, Institut für Immunologie und Transfusionmedizin, Klinikum Sauerbruchstrasse, 17489 Greifswald, Germany
Expert Rev Cardiovasc Ther 2:339-57. 2004..The review concludes with a discussion of pharmacokinetic and clinical data supporting the potential for subcutaneous administration of lepirudin... - The temporal profile of the anti-PF4/heparin immune responseAndreas Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Germany
Blood 113:4970-6. 2009.... - The direct thrombin inhibitor hirudinAndreas Greinacher
Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Sauerbruchstrasse, 17489 Greifswald, Germany
Thromb Haemost 99:819-29. 2008..Drawbacks of hirudin and important precautions for avoiding potential adverse effects are discussed in detail in the third part of this review... - Heparin-induced thrombocytopenia: a stoichiometry-based model to explain the differing immunogenicities of unfractionated heparin, low-molecular-weight heparin, and fondaparinux in different clinical settingsA Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Greifswald Germany
Thromb Res 122:211-20. 2008..We aimed to provide a model that included anticoagulant concentrations and PF4 availability as risk factors influencing the anti-PF4/heparin immune response... - Heparin-induced thrombocytopenia: a prospective study on the incidence, platelet-activating capacity and clinical significance of antiplatelet factor 4/heparin antibodies of the IgG, IgM, and IgA classesA Greinacher
Institut für Immunologie und Transfusionsmedizin Ernst Moritz Arndt Universität Greifswald, Greifswald, Germany
J Thromb Haemost 5:1666-73. 2007..However, the relative utility of functional (platelet activation) vs. antigen [enzyme-immunoassay (EIA)] assays, and the significance of assay discrepancies remain unresolved... - [Thrombosis prophylaxis with heparins in intensive care patients]A Greinacher
Institut für Immunologie und Transfusionsmedizin der Ernst Moritz Arndt Universität Greifswald
Anasthesiol Intensivmed Notfallmed Schmerzther 40:156-63. 2005..Precautions such as monitoring of anticoagulation in patients with renal insufficiency are fundamental if LMWH is given. Further investigations into prevention of venous thromboembolism in ICU patients are urgently needed... - [Recommendations for platelet transfusion by the Joint Thrombocyte Working Party of the German Societies of Transfusion Medicine and Immunohaematology (DGTI), Thrombosis and Haemostasis Research (GTH), and Haematology and Oncology (DGHO)]A Greinacher
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Dtsch Med Wochenschr 131:2675-9. 2006 - Close approximation of two platelet factor 4 tetramers by charge neutralization forms the antigens recognized by HIT antibodiesAndreas Greinacher
Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Sauerbruchstrasse, 17489 Greifswald, Germany
Arterioscler Thromb Vasc Biol 26:2386-93. 2006..To better understand these findings, we analyzed the molecular structure of the complexes formed between PF4 and UFH, LMWH, or fondaparinux... - Clinical features of heparin-induced thrombocytopenia including risk factors for thrombosis. A retrospective analysis of 408 patientsAndreas Greinacher
Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Sauerbruchstr Diagnostikzentrum, 17487 Greifswald, Germany
Thromb Haemost 94:132-5. 2005..HIT-associated thrombosis occurs in a considerable proportion of patients before platelet counts decrease by more than 50%... - Recognition, treatment, and prevention of heparin-induced thrombocytopenia: review and updateAndreas Greinacher
Ernst Moritz Arndt Univerität Greifswald, Institut für Immunologie und Transfusionsmedizin, Germany
Thromb Res 118:165-76. 2006.... - Megakaryocyte impairment by eptifibatide-induced antibodies causes prolonged thrombocytopeniaAndreas Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Blood 114:1250-3. 2009..These findings can be explained by impaired megakaryocytopoiesis complicating anti-GPIIbIIIa antibody-mediated immune thrombocytopenia. This mechanism may also apply to some patients with autoimmune thrombocytopenia... - Heparin-induced thrombocytopeniaA Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Sauerbruchstr, Greifswald, Germany
J Thromb Haemost 7:9-12. 2009..Recent studies of HIT immunobiology suggest that HIT mimics immunity against repetitive antigens, as they are relevant in microbial defense. Thus, understanding HIT may help unravel why host defenses can trigger autoimmunity... - Treatment options for heparin-induced thrombocytopeniaAndreas Greinacher
Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Diagnostikzentrum Saverbruchstrasse, 17487 Greifswald, Germany
Am J Health Syst Pharm 60:S12-8. 2003.... - Association of natural anti-platelet factor 4/heparin antibodies with periodontal diseaseAndreas Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Blood 118:1395-401. 2011..001). Thus, preimmunization to PF4/bacteria complexes by prevalent infections, for example, periodontitis, likely explains the presence of natural anti-PF4/heparin Abs and the early occurrence of anti-PF4/H-IgG in HIT... - Thrombocytopenia in the intensive care unit patientAndreas Greinacher
Institut für Immunologie und Transfusionsmedizin, Universitatsklinikum, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
Hematology Am Soc Hematol Educ Program 2010:135-43. 2010.... - Risk of heparin-induced thrombocytopenia in patients receiving thromboprophylaxisAndreas Greinacher
Institut für Immunologie und Transfusionsmedizin, Sauerbruchstraße, 17487 Greifswald, Germany
Expert Rev Hematol 1:75-85. 2008..Treatment of HIT involves heparin cessation together with anticoagulation with direct thrombin inhibitors or indirect factor Xa inhibitors... - Heparin-induced thrombocytopenia: towards standardization of platelet factor 4/heparin antigen testsA Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
J Thromb Haemost 8:2025-31. 2010..Platelet factor 4 (PF4)/heparin enzyme-immunoassays (EIA) are a widely available surrogate for platelet-activating antibodies... - Antihirudin antibodies following low-dose subcutaneous treatment with desirudin for thrombosis prophylaxis after hip-replacement surgery: incidence and clinical relevanceAndreas Greinacher
Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
Blood 101:2617-9. 2003..However, the total number of immunized patients observed was low and so infrequent (but severe) effects of antihirudin antibodies cannot be excluded... - Impact of demographic changes on the blood supply: Mecklenburg-West Pomerania as a model region for EuropeAndreus Greinacher
Institute for Immunology and Transfusion Medicine, Department of Epidemiology of Health Care and Community Health, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
Transfusion 47:395-401. 2007..The population structure in most European countries is currently changing with a shift from younger to older age groups. This study analyzed how demography will determine future blood demand and supply in a well-characterized region... - Heparin-induced thrombocytopeniaA Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald
Hamostaseologie 30:17-8, 20-8. 2010..Recent studies of HIT immunobiology suggest that HIT mimics immunity against repetitive antigens, as are relevant in microbial defense. Thus, understanding HIT may help unravel why host defenses can trigger autoimmunity... - Characterization of the human neutrophil alloantigen-3aAndreas Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
Nat Med 16:45-8. 2010..The molecular identification of this antigen should facilitate the development of assays for blood donor screening to lower the risk of TRALI... - Anaphylactic and anaphylactoid reactions associated with lepirudin in patients with heparin-induced thrombocytopeniaA Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Klinikum Sauerbruchstrasse, 17489 Greifswald, Germany
Circulation 108:2062-5. 2003..Because 3 cases of fatal anaphylaxis possibly associated with use of lepirudin have been reported, we initiated an investigation of putative lepirudin-associated anaphylaxis... - Treatment of severe neurological deficits with IgG depletion through immunoadsorption in patients with Escherichia coli O104:H4-associated haemolytic uraemic syndrome: a prospective trialAndreas Greinacher
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Lancet 378:1166-73. 2011..Some patients needed ventilatory support due to severe neurological complications, which arose 1 week after onset of enteritis, suggesting an antibody-mediated mechanism. Therefore, we aimed to assess immunoadsorption as rescue therapy... - Incidence and clinical significance of anti-PF4/heparin antibodies of the IgG, IgM, and IgA class in 755 consecutive patient samples referred for diagnostic testing for heparin-induced thrombocytopeniaDavid Juhl
Department of Immunology and Transfusion Medicine, Ernst-Moritz-Arndt University Greifswald, Greifswald, Germany
Eur J Haematol 76:420-6. 2006..Thus, there is a potential for considerable over-diagnosis of HIT by laboratories that utilize only an ELISA for diagnostic testing... - Heparin-induced thrombocytopenia in intensive care patientsKathleen Selleng
Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt Universitat, Greifswald, Germany
Crit Care Med 35:1165-76. 2007.... - Management of anticoagulation in patients with subacute heparin-induced thrombocytopenia scheduled for heart transplantationSixten Selleng
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Blood 112:4024-7. 2008..This can be lifesaving in patients with end-stage heart failure... - The severity of trauma determines the immune response to PF4/heparin and the frequency of heparin-induced thrombocytopeniaNorbert Lubenow
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald
Blood 115:1797-803. 2010..In major trauma certoparin may be safer than UFH because it induces HIT-antibody seroconversion, and the corresponding risk of HIT, less frequently... - Studies of the anti-platelet factor 4/heparin immune response: adapting the enzyme-linked immunosorbent spot assay for detection of memory B cells against complex antigensKathleen Selleng
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Transfusion 50:32-9. 2010.... - The new ID-heparin/PF4 antibody test for rapid detection of heparin-induced antibodies in comparison with functional and antigenic assaysPetra Eichler
Institute for Immunology and Transfusion Medicine, Ernst-Moritz-Arndt-University, Greifswald, Germany
Br J Haematol 116:887-91. 2002..Evaluation of the test in a prospective setting as well as interlaboratory variation should be assessed as a next step... - Heparin-induced thrombocytopenia in intensive care patientsKathleen Selleng
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Semin Thromb Hemost 34:425-38. 2008..Therefore, accurate diagnosis of HIT and individual choice of alternative anticoagulant are important for the adequate management of critically ill HIT patients... - Benefit and risk of heparin for maintaining peripheral venous catheters in neonates: a placebo-controlled trialAnne Friederike Klenner
Department of Pediatrics, the Institute of Immunology and Transfusion Medicine, and the University Pharmacy, Ernst-Moritz-Arndt University, Greifswald, Germany
J Pediatr 143:741-5. 2003..CONCLUSIONS: Balancing the benefits against the risks of heparin addition and the rare complication of HIT, we will not use 0.5 IU/mL heparin addition to parenteral fluids... 
Role of MRP4 (ABCC4) in platelet adenine nucleotide-storage: evidence from patients with delta-storage pool deficienciesGabriele Jedlitschky
Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Institut fur Pharmakologie, Ernst Moritz Arndt University, Friedrich Loeffler Str 23d, D 17487, Greifswald, Germany
Am J Pathol 176:1097-103. 2010..Thus, defective expression of platelet MRP4 is associated with selective defect in AN storage. The genetic basis of the new delta-SPD phenotype remains to be elucidated...- Heparin-induced thrombocytopenia: temporal pattern of thrombocytopenia in relation to initial use or reexposure to heparinNorbert Lubenow
Department of Immunology and Transfusion Medicine, Ernst-Moritz-Arndt University, Greifswald, Germany
Chest 122:37-42. 2002..In contrast, for patients who did not receive heparin within the previous 3 months, HIT is an unlikely explanation for thrombocytopenia that occurs within the first 5 days... - Incidence and clinical relevance of anti-platelet factor 4/heparin antibodies before cardiac surgerySixten Selleng
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Am Heart J 160:362-9. 2010..Whether preoperative presence of antibodies confers adverse prognosis and which particular antibody classes (IgG, IgA, IgM) might be implicated are unknown... - Should patients be informed about the risk of heparin-induced thrombocytopenia before prolonged low-molecular-weight heparin thromboprophylaxis post-trauma/orthopedic surgery?Norbert Lubenow
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Eur J Haematol 79:187-90. 2007..Paradoxically, refusal of thromboprophylaxis as a result of seeking informed consent could increase risk for thrombosis... - Heparin-induced thrombocytopenia and cardiopulmonary bypass: perioperative argatroban useNorbert Lubenow
Institute of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
Ann Thorac Surg 75:577-9. 2003..Because of interference with the international normalized ratio (INR), switching from argatroban to oral anticoagulants is not straightforward... - Immune mechanisms in heparin-induced thrombocytopenia: no evidence for immunoglobulin M anti-idiotype antibodiesKathleen Selleng
Institut für Immunologie und Transfusionsmedizin, Ernst Moritz Arndt Universitat, Greifswald, Germany
Transfusion 49:1812-8. 2009..We hypothesized that these unusual features could be caused by loss of regulatory anti-idiotype IgM antibodies due to disturbance (e.g., by surgery) of an idiotype-anti-idiotype network... - The diagnostic value of the anti-PF4/heparin immunoassay high-dose heparin confirmatory test in cardiac surgery patientsSixten Selleng
Klinik und Poliklinik für Anästhesiologie und Intensivmedizin, Ernst Moritz Arndt Universitat Greifswald, Friedrich Loeffler Strasse 23b, 17475 Greifswald, Germany
Anesth Analg 112:774-6. 2011..Therefore, the high-dose heparin confirmatory test should be used with caution to exclude platelet-activating antiplatelet factor 4/heparin antibodies or clinical HIT... - Heparin-induced thrombocytopenia in children: 12 new cases and review of the literatureAnne F Klenner
, , Sauerbruchstr./Diagnostikzentrum, 17487 Greifswald, Germany
Thromb Haemost 91:719-24. 2004..Most data are available for lepirudin and danaparoid. Substitution of unfractionated heparin by low-molecular-weight heparins for regular anticoagulation may reduce the incidence of HIT... - Immune heparin-induced thrombocytopenia can occur in patients receiving clopidogrel and aspirinKathleen Selleng
Institute of Immunology and Transfusion Medicine, Ernst-Moritz-Arndt University, Greifswald, Germany
Am J Hematol 78:188-92. 2005..We conclude that combined therapy with aspirin and clopidogrel does not necessarily protect against clinical HIT, at least in patients with HIT antibodies that have strong platelet-activating characteristics... - Antibodies against lepirudin are polyspecific and recognize epitopes on bivalirudinPetra Eichler
Institute for Immunology and Transfusion Medicine, Ernst-Moritz-Arndt-University, Sauerbruchstr/Diagnostikzentrum, 17487 Greifswald, Germany
Blood 103:613-6. 2004..Yet, as lepirudin and antilepirudin antibodies have recently been implicated in severe anaphylactic reactions, caution is warranted when using bivalirudin in patients previously treated with lepirudin... - Ceftriaxone causes drug-induced immune thrombocytopenia and hemolytic anemia: characterization of targets on platelets and red blood cellsBeatrice Grossjohann
Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Germany
Transfusion 44:1033-40. 2004..In addition, the DDAbs were dependent on the substitution at position 3 of the ceftriaxone molecule. Epitopes on GPIIb/IIIa and GPIX were involved on PLTs. The Rh protein was not the only target of DDAbs on RBCs... - Very severe thrombocytopenia and fragmentation hemolysis mimicking thrombotic thrombocytopenic purpura associated with a giant intracardiac vegetation infected with Staphylococcus epidermidis: role of monocyte procoagulant activity induced by bacterial suKathleen Selleng
Department of Transfusion Medicine and Immunology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
Am J Hematol 82:766-71. 2007.... - Platelet receptor and clotting factor polymorphisms as genetic risk factors for thromboembolic complications in heparin-induced thrombocytopeniaLena E Carlsson
Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
Pharmacogenetics 13:253-8. 2003..However, this would need to be assessed in further larger studies. Most likely, the high levels of thrombin generation during acute HIT are so procoagulant that less pronounced risk factors such as polymorphisms are overshadowed... - Results of a systematic evaluation of treatment outcomes for heparin-induced thrombocytopenia in patients receiving danaparoid, ancrod, and/or coumarin explain the rapid shift in clinical practice during the 1990sNorbert Lubenow
Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
Thromb Res 117:507-15. 2006..During the 1990s, danaparoid+/-coumarin began to replace ancrod (+/-coumarin), or coumarin alone, for treating HIT, despite danaparoid not being approved for treatment of HIT... - Human platelets express organic anion-transporting peptide 2B1, an uptake transporter for atorvastatinJuliane Niessen
Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Friedrich Loeffler Str 23 D, D 17487 Greifswald, Germany
Drug Metab Dispos 37:1129-37. 2009..In conclusion, OATP2B1 is an uptake transporter expressed in platelets and is involved in statin-mediated alteration of platelet aggregation... - Hematopoietic stem cell differentiation affects expression and function of MRP4 (ABCC4), a transport protein for signaling molecules and drugsLena Oevermann
Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Greifswald, Germany
Int J Cancer 124:2303-11. 2009.... - The nucleotide transporter MRP4 (ABCC4) is highly expressed in human platelets and present in dense granules, indicating a role in mediator storageGabriele Jedlitschky
Department of Pharmacology, Peter Holtz Research Center of Pharmacology and Experimental Therapeutics, Greifswald, Germany
Blood 104:3603-10. 2004..The results indicate a function of MRP4 in platelet mediator storage and inhibition of MRP4 may represent a novel mechanism for inhibition of platelet function by some anti-inflammatory drugs... - Subfractionation and purification of intracellular granule-structures of human platelets: an improved method based on magnetic sortingJuliane Niessen
Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Greifswald, Germany
J Immunol Methods 328:89-96. 2007..Therefore, studies requiring highly purified material (e.g. identification of specific transporters and receptors) will benefit from these results... - Perioperative management of MYH9 hereditary macrothrombocytopenia (Fechtner syndrome)Kathleen Selleng
Department of Transfusion Medicine and Immunology, Ernst Moritz Arndt University, Greifswald, Germany
Eur J Haematol 79:263-8. 2007..A major risk for these patients can be inappropriate treatment with long-term corticosteroids or splenectomy for misdiagnosed chronic autoimmune thrombocytopenia, as well as inadequate peri- and postoperative management... - The adhesion and spreading of thrombocyte vesicles on electrode surfacesVíctor Agmo Hernández
Institut fur Biochemie, Universitat Greifswald, Felix Hausdorff Str 4, D 17487, Greifswald, Germany
Bioelectrochemistry 74:210-6. 2008..The chronoamperometric measurements indicate the possibility of future applications to probe membrane properties of thrombocytes... - Fc(gamma) receptors IIa on cardiomyocytes and their potential functional relevance in dilated cardiomyopathyAlexander Staudt
Klinik für Innere Medizin B, Ernst Moritz Arndt Universitat, Greifswald, Germany
J Am Coll Cardiol 49:1684-92. 2007..The purpose of this study was to investigate how cardiac autoantibodies might contribute to cardiac dysfunction in patients suffering from dilated cardiomyopathy (DCM)... - Proteomics of blood-based therapeutics: a promising tool for quality assurance in transfusion medicineThomas Thiele
Institute of Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
BioDrugs 21:179-93. 2007..Optimized preparation procedures and storage conditions will reduce the risk of protein alterations, which in turn may contribute to better recovery, reduced exposure to allogeneic proteins, and increased transfusion safety... - Transfusion medicine in the era of genomics and proteomicsAndreas Greinacher
Department of Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
Transfus Med Rev 19:288-94. 2005..By combining proteomics and immunohematology, those manufacturing processes least likely to generate posttranslational protein modifications will need to be identified... - Profiling of alterations in platelet proteins during storage of platelet concentratesThomas Thiele
Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University, Greifswald, Germany
Transfusion 47:1221-33. 2007..In contrast, proteomic technologies provide a comprehensive overview of the PLT proteome. High interassay variability, however, limits meaningful assessment of samples taken from the same product over time or before and after processing... - Isolation of platelet granulesJuliane Niessen
Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Greifswald, Germany
Curr Protoc Cell Biol . 2010..The immunomagnetic sorting method is especially good for studies requiring highly purified material (e.g., for the identification of specific transporters and receptors)... - Expression of ABC-type transport proteins in human plateletsJuliane Niessen
Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics, Ernst Moritz Arndt University, Greifswald, Germany
Pharmacogenet Genomics 20:396-400. 2010..Their functions may be related to glutathione and lipid homeostasis, secretion of lipid mediators, cell protection as well as drug transport... - So, does low-molecular-weight heparin cause less heparin-induced thrombocytopenia than unfractionated heparin or not?Theodore E Warkentin
Chest 132:1108-10. 2007 - Thrombosis of the cerebral veins and sinusesTheodore E Warkentin
N Engl J Med 353:314-5. 2005 - Heparin-induced thrombocytopenia and cardiac surgeryTheodore E Warkentin
Department of Pathology, McMaster University, Hamilton, Ontario, Canada
Ann Thorac Surg 76:2121-31. 2003..As heparin-induced thrombocytopenia antibodies are transient, unfractionated heparin alone is appropriate in a patient with previous heparin-induced thrombocytopenia whose antibodies have disappeared... - Lepirudin for prophylaxis of thrombosis in patients with acute isolated heparin-induced thrombocytopenia: an analysis of 3 prospective studiesNorbert Lubenow
Dept of Immunology and Transfusion Medicine, Ernst-Moritz-Arndt University Greifswald, Germany
Blood 104:3072-7. 2004..Dose reductions in renal impairment are important. Keeping the aPTT in the range corresponding to 600 to 700 microg/L lepirudin during treatment may minimize bleeding complications... - Contaminated heparinAndreas Greinacher
N Engl J Med 359:1291-2; author reply 1293. 2008 - Differences in the clinically effective molar concentrations of four direct thrombin inhibitors explain their variable prothrombin time prolongationTheodore E Warkentin
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
Thromb Haemost 94:958-64. 2005..DTIs with a relatively low affinity for thrombin require high plasma concentrations to double the APTT; these higher plasma concentrations, in turn, quench more of the thrombin generated in the PT, thereby more greatly prolonging the PT... - Antigen-positive platelet transfusion in neonatal alloimmune thrombocytopenia (NAIT)Volker Kiefel
Department of Transfusion Medicine, University of Rostock, Ernst Heydemann Strasse 6, D 18057 Rostock, Germany
Blood 107:3761-3. 2006..We conclude that transfusion of platelet concentrates from random donors is an appropriate strategy in the management of unexpected, severe NAIT predominantly in first pregnancies, pending the availability of compatible platelets... - Replacement of unfractionated heparin by low-molecular-weight heparin for postorthopedic surgery antithrombotic prophylaxis lowers the overall risk of symptomatic thrombosis because of a lower frequency of heparin-induced thrombocytopeniaAndreas Greinacher
Blood 106:2921-2. 2005 - Heparin-induced thrombocytopenia in patients receiving mechanical circulatory supportSoren Schenk
Thoracic and Cardiovascular Surgery, Heart Center North Rhine-Westphalia, , Bad Oeynhausen, Germany
J Thorac Cardiovasc Surg 131:1373-81.e4. 2006..Frequent antibody screening is recommended due to the increased risk of thromboembolism. Heparin alternatives should be subjected to clinical trials in these high-risk patients... - Anti-platelet factor 4/heparin antibodies in orthopedic surgery patients receiving antithrombotic prophylaxis with fondaparinux or enoxaparinTheodore E Warkentin
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada
Blood 106:3791-6. 2005..We conclude that despite similar immunogenicity of fondaparinux and LMWH, PF4/fondaparinux, but not PF4/LMWH, is recognized poorly by the antibodies generated, suggesting that the risk of HIT with fondaparinux likely is very low... - Management of liver transplantation in a patient with a history of heparin-induced thrombocytopeniaReinhold Fretschner
Department of Anesthesiology and Critical Care, University Hospital Tubingen, Tubingen, Germany
Transpl Int 18:664-7. 2005..Twenty months after an episode of HIT, a patient may receive an organ from a heparin-treated donor without risk of thrombocytopenia or thromboembolic complications. Avoidance of heparin for postoperative anticoagulation is recommended... - Effect of fondaparinux on platelet activation in the presence of heparin-dependent antibodies: a blinded comparative multicenter study with unfractionated heparinPierre Savi
Cardiovascular Thrombosis Research Department, Sanofi Synthelabo Recherche, Toulouse, France
Blood 105:139-44. 2005..Together, these results suggest that fondaparinux is nonreactive to HIT sera and raise the possibility that the drug may be used for prophylaxis and treatment of thrombosis in patients with a history of HIT... - HIT happens: diagnosing and evaluating the patient with heparin-induced thrombocytopeniaAndreas Greinacher
Anesth Analg 107:356-8. 2008 - Heparin-induced thrombocytopenia and cardiac surgeryTheodore E Warkentin
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
Ann Thorac Surg 76:638-48. 2003..As heparin-induced thrombocytopenia antibodies are transient, unfractionated heparin alone is appropriate in a patient with previous heparin-induced thrombocytopenia whose antibodies have disappeared... - Heparin-induced thrombocytopenia: recognition, treatment, and prevention: the Seventh ACCP Conference on Antithrombotic and Thrombolytic TherapyTheodore E Warkentin
Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, General Site, 237 Barton St E, Hamilton, Ontario L8L 2X2, Canada
Chest 126:311S-337S. 2004.... - BivalirudinTheodore E Warkentin
Department of Pathology and Molecular Medicine, Michael G DeGroote School of Medicine, McMaster Unniversity, Hamilton, Ontario, L8L 2X2 Canada
Thromb Haemost 99:830-9. 2008.... - Treatment and prevention of heparin-induced thrombocytopenia: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines (8th Edition)Theodore E Warkentin
Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, 237 Barton Street E, Hamilton, Ontario, Canada
Chest 133:340S-380S. 2008..For patients receiving VKAs at the time of diagnosis of HIT, we recommend use of vitamin K (10 mg po or 5 to 10 mg IV) [Grade 1C]... - Postoperative complications after cardiac surgery and HIT: a word of cautionFederico Pappalardo
Ann Thorac Surg 86:1054-5; author reply 1055. 2008 - A variant of the Sebastian platelet syndrome with unique neutrophil inclusionsJames G White
University of Minnesota School of Medicine, Menneapolis, MN 55455, USA
Platelets 13:121-7. 2002.... - Heparin-induced thrombocytopenia: a complication in extracorporeal photochemotherapy (photopheresis)Thomas Dittberner
J Am Acad Dermatol 47:452-3. 2002 - Too many hits in HIT?Andreas Greinacher
Am J Hematol 82:1035-6. 2007 - The risk of transfusion to the wrong patient in GermanyGregor Caspari
Transfusion 42:1238-9. 2002 - A systematic review and survey of the management of unexpected neonatal alloimmune thrombocytopeniaDirk Bassler
Department of Pediatrics, Neonatal Division, McMaster University, Hamilton, Ontario, Canada
Transfusion 48:92-8. 2008..Unexpected neonatal alloimmune thrombocytopenia (NAIT) may have devastating consequences and its management is challenging. To design future trials, evidence from the literature and existing best practice need review... - Iron deficiency anaemia in young womenLena E Carlsson
Department of Immunology and Transfusion Medicine, Ernst-Moritz-Arndt-University, Greifswald, Germany
Eur J Haematol 68:341-4. 2002..5% vs. 33.1%, P=0.003). CONCLUSION: Iron deficiency anaemia in young women might have been the evolutionary disadvantage causing the gene shift from GPIa-807CC to 807CT...