Sandro Ghisla

Summary

Affiliation: University of Konstanz
Country: Germany

Publications

  1. pmc Reduced flavin: NMR investigation of N5-H exchange mechanism, estimation of ionisation constants and assessment of properties as biological catalyst
    Peter Macheroux
    Graz University of Technology, Institute of Biochemistry, Petersgasse 12, A 8010 Graz, Austria
    BMC Biochem 6:26. 2005
  2. ncbi request reprint Acyl-CoA dehydrogenases. A mechanistic overview
    Sandro Ghisla
    Department of Biology, University of Konstanz, Germany
    Eur J Biochem 271:494-508. 2004
  3. ncbi request reprint Beta-oxidation of fatty acids. A century of discovery
    Sandro Ghisla
    Department of Biology, University of Konstanz, Germany
    Eur J Biochem 271:459-61. 2004
  4. ncbi request reprint Radical phosphate transfer mechanism for the thiamin diphosphate- and FAD-dependent pyruvate oxidase from Lactobacillus plantarum. Kinetic coupling of intercofactor electron transfer with phosphate transfer to acetyl-thiamin diphosphate via a transient FA
    Kai Tittmann
    Institut fur Biochemie, Martin Luther Universitat Halle Wittenberg, Kurt Mothes Strasse 3, D 06120 Halle Saale, Germany
    Biochemistry 44:13291-303. 2005
  5. pmc Structural and kinetic analyses of the H121A mutant of cholesterol oxidase
    Louis Lim
    Department of Chemistry and Biochemistry, Sinsheimer Laboratory, University of California at Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA
    Biochem J 400:13-22. 2006
  6. ncbi request reprint Identification of isobutyryl-CoA dehydrogenase and its deficiency in humans
    Tien V Nguyen
    Department of Medical Genetics, Mayo Clinic, Rochester, MN 55905, USA
    Mol Genet Metab 77:68-79. 2002
  7. ncbi request reprint On the mechanism of Rhodotorula gracilis D-amino acid oxidase: role of the active site serine 335
    Angelo Boselli
    Department of Structural and Functional Biology, University of Insubria, via JH Dunant 3, 21100 Varese, Italy
    Biochim Biophys Acta 1702:19-32. 2004
  8. pmc On the oxygen reactivity of flavoprotein oxidases: an oxygen access tunnel and gate in brevibacterium sterolicum cholesterol oxidase
    Luciano Piubelli
    Department of Biotechnology and Molecular Sciences, University of Insubria, 21100 Varese, Italy
    J Biol Chem 283:24738-47. 2008

Collaborators

  • Georg Wille
  • Jerry Vockley
  • Peter Macheroux
  • Kai Tittmann
  • Luciano Piubelli
  • Loredano Pollegioni
  • Gianluca Molla
  • Louis Lim
  • Mirella S Pilone
  • Angelo Boselli
  • Tien V Nguyen
  • Susanne Feindler-Boeckh
  • Mattia Pedotti
  • Nicole Guinn
  • Alice Vrielink
  • Silvia Sacchi
  • Nicolas J Lench
  • Brage S Andresen
  • Thomas J Corydon
  • Stephen D Cederbaum
  • Diane S Roe
  • Nasser Abd-El Razik
  • Al Walid A Mohsen
  • Charles R Roe

Detail Information

Publications8

  1. pmc Reduced flavin: NMR investigation of N5-H exchange mechanism, estimation of ionisation constants and assessment of properties as biological catalyst
    Peter Macheroux
    Graz University of Technology, Institute of Biochemistry, Petersgasse 12, A 8010 Graz, Austria
    BMC Biochem 6:26. 2005
    ..The present work addresses properties of reduced flavin relevant in this context...
  2. ncbi request reprint Acyl-CoA dehydrogenases. A mechanistic overview
    Sandro Ghisla
    Department of Biology, University of Konstanz, Germany
    Eur J Biochem 271:494-508. 2004
    ..These views are also discussed in the context of the accompanying paper on the three-dimensional properties of acyl-CoA dehydrogenases...
  3. ncbi request reprint Beta-oxidation of fatty acids. A century of discovery
    Sandro Ghisla
    Department of Biology, University of Konstanz, Germany
    Eur J Biochem 271:459-61. 2004
  4. ncbi request reprint Radical phosphate transfer mechanism for the thiamin diphosphate- and FAD-dependent pyruvate oxidase from Lactobacillus plantarum. Kinetic coupling of intercofactor electron transfer with phosphate transfer to acetyl-thiamin diphosphate via a transient FA
    Kai Tittmann
    Institut fur Biochemie, Martin Luther Universitat Halle Wittenberg, Kurt Mothes Strasse 3, D 06120 Halle Saale, Germany
    Biochemistry 44:13291-303. 2005
    ..Thus, LpPOX would constitute the first example of a radical-based phosphorolysis mechanism in biochemistry...
  5. pmc Structural and kinetic analyses of the H121A mutant of cholesterol oxidase
    Louis Lim
    Department of Chemistry and Biochemistry, Sinsheimer Laboratory, University of California at Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA
    Biochem J 400:13-22. 2006
    ..Since a similar distortion of the flavin has not been observed in other covalent flavoproteins, it is proposed to represent a specific mode to facilitate flavin reduction in covalent cholesterol oxidase...
  6. ncbi request reprint Identification of isobutyryl-CoA dehydrogenase and its deficiency in humans
    Tien V Nguyen
    Department of Medical Genetics, Mayo Clinic, Rochester, MN 55905, USA
    Mol Genet Metab 77:68-79. 2002
    ....
  7. ncbi request reprint On the mechanism of Rhodotorula gracilis D-amino acid oxidase: role of the active site serine 335
    Angelo Boselli
    Department of Structural and Functional Biology, University of Insubria, via JH Dunant 3, 21100 Varese, Italy
    Biochim Biophys Acta 1702:19-32. 2004
    ..9 +/- 0.4. Interestingly, the substitution suppresses the activity towards d-lactate, suggesting a role of the serine 335 in removal of the substrate alpha-OH hydrogen...
  8. pmc On the oxygen reactivity of flavoprotein oxidases: an oxygen access tunnel and gate in brevibacterium sterolicum cholesterol oxidase
    Luciano Piubelli
    Department of Biotechnology and Molecular Sciences, University of Insubria, 21100 Varese, Italy
    J Biol Chem 283:24738-47. 2008
    ..This is taken as support for the assumption that residue Glu-311 finely tunes the Glu-475-Arg-477 pair, forming a gate that functions in modulating the access/reactivity of dioxygen...