Niels H Gehring

Summary

Affiliation: University of Heidelberg
Country: Germany

Publications

  1. pmc The uORF-containing thrombopoietin mRNA escapes nonsense-mediated decay (NMD)
    Clemens Stockklausner
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, 69120 Heidelberg, Germany
    Nucleic Acids Res 34:2355-63. 2006
  2. pmc The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decay
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 7:e1000120. 2009
  3. pmc Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA decay and translation
    Joachim B Kunz
    Department for Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    RNA 12:1015-22. 2006
  4. ncbi request reprint Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg 69120, Germany
    Mol Cell 20:65-75. 2005
  5. pmc Interactions between UPF1, eRFs, PABP and the exon junction complex suggest an integrated model for mammalian NMD pathways
    Pavel V Ivanov
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    EMBO J 27:736-47. 2008
  6. doi request reprint Disassembly of exon junction complexes by PYM
    Niels H Gehring
    University of Heidelberg and European Molecular Biology Laboratory, Germany
    Cell 137:536-48. 2009
  7. pmc The abundance of RNPS1, a protein component of the exon junction complex, can determine the variability in efficiency of the Nonsense Mediated Decay pathway
    Marcelo H Viegas
    Department of Pediatric Oncology, Hematology and Immunology, Children s Hospital, University of Heidelberg, Im Neuenheimer Feld 150, 69120 Heidelberg, Germany
    Nucleic Acids Res 35:4542-51. 2007
  8. ncbi request reprint A chemiluminescence-based reporter system to monitor nonsense-mediated mRNA decay
    Stephanie Boelz
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Im Neuenheimer Feld 156, 69120 Heidelberg, Germany
    Biochem Biophys Res Commun 349:186-91. 2006
  9. pmc Internal ribosome entry sequence-mediated translation initiation triggers nonsense-mediated decay
    Jill A Holbrook
    European Molecular Biology Laboratory, University Hospital Heidelberg, Molecular Medicine Partnership Unit, University of Heidelberg, Im Neuenheimer Feld 150, Heidelberg 69120, Germany
    EMBO Rep 7:722-6. 2006
  10. pmc Splicing factors stimulate polyadenylation via USEs at non-canonical 3' end formation signals
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    EMBO J 26:2658-69. 2007

Collaborators

Detail Information

Publications18

  1. pmc The uORF-containing thrombopoietin mRNA escapes nonsense-mediated decay (NMD)
    Clemens Stockklausner
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, 69120 Heidelberg, Germany
    Nucleic Acids Res 34:2355-63. 2006
    ..Thus, regulation of TPO expression is independent of NMD, implying that mRNAs bearing uORFs cannot generally be considered to represent NMD targets...
  2. pmc The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decay
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 7:e1000120. 2009
    ..Based on this systematic analysis of EJC assembly by the spliceosome, we propose a model of how a functional EJC is assembled in a strictly sequential and hierarchical fashion, including nuclear splicing-dependent and cytoplasmic steps...
  3. pmc Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA decay and translation
    Joachim B Kunz
    Department for Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    RNA 12:1015-22. 2006
    ..Stimulation of translation is independent of this interaction and is determined by other regions of the hUpf3 protein, indicating the presence of different downstream pathways of hUpf3 proteins either in NMD or in translation...
  4. ncbi request reprint Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg 69120, Germany
    Mol Cell 20:65-75. 2005
    ..These results are integrated into a nonlinear model for mammalian NMD involving alternative routes of entry that converge at a common requirement of UPF1...
  5. pmc Interactions between UPF1, eRFs, PABP and the exon junction complex suggest an integrated model for mammalian NMD pathways
    Pavel V Ivanov
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    EMBO J 27:736-47. 2008
    ..The EJC, with UPF2 or UPF3b as a cofactor, interferes with physiological termination through UPF1. This model integrates previously competing models of NMD and suggests a mechanistic basis for alternative NMD pathways...
  6. doi request reprint Disassembly of exon junction complexes by PYM
    Niels H Gehring
    University of Heidelberg and European Molecular Biology Laboratory, Germany
    Cell 137:536-48. 2009
    ..In cells depleted of PYM, EJCs accumulate on spliced mRNAs and EJC protein recycling is impaired. Hence, PYM is an EJC disassembly factor that acts both in vitro and in living cells, and that antagonizes important EJC functions...
  7. pmc The abundance of RNPS1, a protein component of the exon junction complex, can determine the variability in efficiency of the Nonsense Mediated Decay pathway
    Marcelo H Viegas
    Department of Pediatric Oncology, Hematology and Immunology, Children s Hospital, University of Heidelberg, Im Neuenheimer Feld 150, 69120 Heidelberg, Germany
    Nucleic Acids Res 35:4542-51. 2007
    ..We conclude that cellular concentrations of RNPS1 can modify NMD efficiency and propose that cell type specific co-factor availability represents a novel principle that controls NMD...
  8. ncbi request reprint A chemiluminescence-based reporter system to monitor nonsense-mediated mRNA decay
    Stephanie Boelz
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Im Neuenheimer Feld 156, 69120 Heidelberg, Germany
    Biochem Biophys Res Commun 349:186-91. 2006
    ..Wortmannin treatment enhanced NMD reporter expression in our system in a dose-dependent way, illustrating its utility for small molecule screening...
  9. pmc Internal ribosome entry sequence-mediated translation initiation triggers nonsense-mediated decay
    Jill A Holbrook
    European Molecular Biology Laboratory, University Hospital Heidelberg, Molecular Medicine Partnership Unit, University of Heidelberg, Im Neuenheimer Feld 150, Heidelberg 69120, Germany
    EMBO Rep 7:722-6. 2006
    ..These data generalize the previous model and suggest that translation per se, irrespective of how it is initiated, can mediate NMD...
  10. pmc Splicing factors stimulate polyadenylation via USEs at non-canonical 3' end formation signals
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    EMBO J 26:2658-69. 2007
    ..These data uncover a novel mechanism that functionally links the splicing and 3' end formation machineries of multiple cellular mRNAs in an USE-dependent manner...
  11. pmc Mechanism of escape from nonsense-mediated mRNA decay of human beta-globin transcripts with nonsense mutations in the first exon
    Gabriele Neu-Yilik
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    RNA 17:843-54. 2011
    ..Furthermore, our data uncover a reason why the position of a nonsense mutation alone does not suffice to predict the fate of the affected mRNA and its effect on protein expression...
  12. ncbi request reprint The prothrombin 3'end formation signal reveals a unique architecture that is sensitive to thrombophilic gain-of-function mutations
    Sven Danckwardt
    Molecular Medicine Partnership Unit, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
    Blood 104:428-35. 2004
    ..This balance appears to be highly susceptible to being disturbed by clinically relevant gain-of-function mutations...
  13. pmc Nonsense-mediated mRNA decay: from vacuum cleaner to Swiss army knife
    Gabriele Neu-Yilik
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Im Neuenheimer Feld 150, 69120 Heidelberg, Germany
    Genome Biol 5:218. 2004
    ..Two new approaches have identified physiological NMD substrates, and suggest that NMD functions as a multipurpose tool in the modulation of gene expression...
  14. ncbi request reprint Y14 and hUpf3b form an NMD-activating complex
    Niels H Gehring
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    Mol Cell 11:939-49. 2003
    ..These results uncover a direct role of Y14 in NMD and suggest an unexpected hierarchy in the assembly of NMD complexes...
  15. ncbi request reprint 3' end processing of the prothrombin mRNA in thrombophilia
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    Acta Haematol 115:192-7. 2006
    ....
  16. ncbi request reprint The human intronless melanocortin 4-receptor gene is NMD insensitive
    Katja S Brocke
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Im Neuenheimer Feld 150, D 69120 Heidelberg, Germany
    Hum Mol Genet 11:331-5. 2002
    ..Thus, the naturally intronless MC4-R gene and probably many other intronless genes fail to be monitored by the NMD pathway...
  17. doi request reprint Tethering assays to investigate nonsense-mediated mRNA decay activating proteins
    Niels H Gehring
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    Methods Enzymol 448:467-82. 2008
    ..In this chapter we explicate the cloning of appropriate reporter plasmids and the setup of a tethering experiment with the necessary control experiments. Advantages of the different systems and tags are discussed...
  18. pmc NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins
    Bhagyashri D Burgute
    Institute of Biochemistry I, Medical Faculty, Center for Molecular Medicine Cologne CMMC, 50931 Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases CECAD, University of Cologne, Cologne, Germany, Institute of Genetics, University of Cologne, 50931 Cologne, Germany and Leibniz Institute of Freshwater Ecology and Inland Fisheries, IGB, Muggelseedamm 301, 12587 Berlin, Germany
    Nucleic Acids Res 42:3177-93. 2014
    ..Our results reveal NKAP as nuclear speckle protein with roles in RNA splicing and processing. ..