Niels H Gehring

Summary

Affiliation: University of Heidelberg
Country: Germany

Publications

  1. ncbi The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decay
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 7:e1000120. 2009
  2. ncbi Disassembly of exon junction complexes by PYM
    Niels H Gehring
    University of Heidelberg and European Molecular Biology Laboratory, Germany
    Cell 137:536-48. 2009
  3. ncbi A chemiluminescence-based reporter system to monitor nonsense-mediated mRNA decay
    Stephanie Boelz
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Im Neuenheimer Feld 156, 69120 Heidelberg, Germany
    Biochem Biophys Res Commun 349:186-91. 2006
  4. ncbi Interactions between UPF1, eRFs, PABP and the exon junction complex suggest an integrated model for mammalian NMD pathways
    Pavel V Ivanov
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    EMBO J 27:736-47. 2008
  5. ncbi Mechanism of escape from nonsense-mediated mRNA decay of human beta-globin transcripts with nonsense mutations in the first exon
    Gabriele Neu-Yilik
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    RNA 17:843-54. 2011
  6. ncbi Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg 69120, Germany
    Mol Cell 20:65-75. 2005
  7. ncbi Internal ribosome entry sequence-mediated translation initiation triggers nonsense-mediated decay
    Jill A Holbrook
    European Molecular Biology Laboratory, University Hospital Heidelberg, Molecular Medicine Partnership Unit, University of Heidelberg, Im Neuenheimer Feld 150, Heidelberg 69120, Germany
    EMBO Rep 7:722-6. 2006
  8. ncbi 3' end processing of the prothrombin mRNA in thrombophilia
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    Acta Haematol 115:192-7. 2006
  9. ncbi Tethering assays to investigate nonsense-mediated mRNA decay activating proteins
    Niels H Gehring
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    Methods Enzymol 448:467-82. 2008
  10. ncbi The prothrombin 3'end formation signal reveals a unique architecture that is sensitive to thrombophilic gain-of-function mutations
    Sven Danckwardt
    Molecular Medicine Partnership Unit, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
    Blood 104:428-35. 2004

Collaborators

  • Matthias W Hentze
  • Gabriele Neu-Yilik
  • Andreas E Kulozik
  • Sven Danckwardt
  • Pavel V Ivanov
  • Stephanie Boelz
  • Jill A Holbrook
  • Joachim B Kunz
  • Kathrin Hartmann
  • Margit Pforsich
  • Patrick Hundsdoerfer
  • Ute Frede

Detail Information

Publications10

  1. ncbi The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decay
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 7:e1000120. 2009
    ..Based on this systematic analysis of EJC assembly by the spliceosome, we propose a model of how a functional EJC is assembled in a strictly sequential and hierarchical fashion, including nuclear splicing-dependent and cytoplasmic steps...
  2. ncbi Disassembly of exon junction complexes by PYM
    Niels H Gehring
    University of Heidelberg and European Molecular Biology Laboratory, Germany
    Cell 137:536-48. 2009
    ..In cells depleted of PYM, EJCs accumulate on spliced mRNAs and EJC protein recycling is impaired. Hence, PYM is an EJC disassembly factor that acts both in vitro and in living cells, and that antagonizes important EJC functions...
  3. ncbi A chemiluminescence-based reporter system to monitor nonsense-mediated mRNA decay
    Stephanie Boelz
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Im Neuenheimer Feld 156, 69120 Heidelberg, Germany
    Biochem Biophys Res Commun 349:186-91. 2006
    ..Wortmannin treatment enhanced NMD reporter expression in our system in a dose-dependent way, illustrating its utility for small molecule screening...
  4. ncbi Interactions between UPF1, eRFs, PABP and the exon junction complex suggest an integrated model for mammalian NMD pathways
    Pavel V Ivanov
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    EMBO J 27:736-47. 2008
    ..The EJC, with UPF2 or UPF3b as a cofactor, interferes with physiological termination through UPF1. This model integrates previously competing models of NMD and suggests a mechanistic basis for alternative NMD pathways...
  5. ncbi Mechanism of escape from nonsense-mediated mRNA decay of human beta-globin transcripts with nonsense mutations in the first exon
    Gabriele Neu-Yilik
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    RNA 17:843-54. 2011
    ..Furthermore, our data uncover a reason why the position of a nonsense mutation alone does not suffice to predict the fate of the affected mRNA and its effect on protein expression...
  6. ncbi Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements
    Niels H Gehring
    Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg 69120, Germany
    Mol Cell 20:65-75. 2005
    ..These results are integrated into a nonlinear model for mammalian NMD involving alternative routes of entry that converge at a common requirement of UPF1...
  7. ncbi Internal ribosome entry sequence-mediated translation initiation triggers nonsense-mediated decay
    Jill A Holbrook
    European Molecular Biology Laboratory, University Hospital Heidelberg, Molecular Medicine Partnership Unit, University of Heidelberg, Im Neuenheimer Feld 150, Heidelberg 69120, Germany
    EMBO Rep 7:722-6. 2006
    ..These data generalize the previous model and suggest that translation per se, irrespective of how it is initiated, can mediate NMD...
  8. ncbi 3' end processing of the prothrombin mRNA in thrombophilia
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    Acta Haematol 115:192-7. 2006
    ....
  9. ncbi Tethering assays to investigate nonsense-mediated mRNA decay activating proteins
    Niels H Gehring
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    Methods Enzymol 448:467-82. 2008
    ..In this chapter we explicate the cloning of appropriate reporter plasmids and the setup of a tethering experiment with the necessary control experiments. Advantages of the different systems and tags are discussed...
  10. ncbi The prothrombin 3'end formation signal reveals a unique architecture that is sensitive to thrombophilic gain-of-function mutations
    Sven Danckwardt
    Molecular Medicine Partnership Unit, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
    Blood 104:428-35. 2004
    ..This balance appears to be highly susceptible to being disturbed by clinically relevant gain-of-function mutations...