Genomes and Genes
Niels H Gehring
Affiliation: University of Heidelberg
- Disassembly of exon junction complexes by PYMNiels H Gehring
University of Heidelberg and European Molecular Biology Laboratory, Germany
Cell 137:536-48. 2009..In cells depleted of PYM, EJCs accumulate on spliced mRNAs and EJC protein recycling is impaired. Hence, PYM is an EJC disassembly factor that acts both in vitro and in living cells, and that antagonizes important EJC functions...
- The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decayNiels H Gehring
Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany
PLoS Biol 7:e1000120. 2009..Based on this systematic analysis of EJC assembly by the spliceosome, we propose a model of how a functional EJC is assembled in a strictly sequential and hierarchical fashion, including nuclear splicing-dependent and cytoplasmic steps...
- Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirementsNiels H Gehring
Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg 69120, Germany
Mol Cell 20:65-75. 2005..These results are integrated into a nonlinear model for mammalian NMD involving alternative routes of entry that converge at a common requirement of UPF1...
- A chemiluminescence-based reporter system to monitor nonsense-mediated mRNA decayStephanie Boelz
Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Im Neuenheimer Feld 156, 69120 Heidelberg, Germany
Biochem Biophys Res Commun 349:186-91. 2006..Wortmannin treatment enhanced NMD reporter expression in our system in a dose-dependent way, illustrating its utility for small molecule screening...