Georg Fuchs

Summary

Affiliation: University of Freiburg
Country: Germany

Publications

  1. doi request reprint Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota
    Ivan A Berg
    Mikrobiologie, Fakultat Biologie, Albert Ludwigs Universitat Freiburg, Germany
    Microbiology 156:256-69. 2010
  2. ncbi request reprint New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii
    Annette Zaar
    Institut fur Biologie II, Mikrobiologie, Universitat Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    Mol Microbiol 54:223-38. 2004
  3. doi request reprint Anaerobic metabolism of aromatic compounds
    Georg Fuchs
    Microbiology, Faculty of Biology, University of Freiburg, Schaenzelstr 1, D 79104 Freiburg, Germany
    Ann N Y Acad Sci 1125:82-99. 2008
  4. doi request reprint Microbial degradation of aromatic compounds - from one strategy to four
    Georg Fuchs
    Mikrobiologie, Universitat Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    Nat Rev Microbiol 9:803-16. 2011
  5. doi request reprint Alternative pathways of carbon dioxide fixation: insights into the early evolution of life?
    Georg Fuchs
    Lehrstuhl Mikrobiologie, Universitat Freiburg, D 79104 Freiburg, Germany
    Annu Rev Microbiol 65:631-58. 2011
  6. pmc Labeling and enzyme studies of the central carbon metabolism in Metallosphaera sedula
    Sebastian Estelmann
    Mikrobiologie, Fakultat fur Biologie, Freiburg, Germany
    J Bacteriol 193:1191-200. 2011
  7. pmc Properties of succinyl-coenzyme A:D-citramalate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus
    Silke Friedmann
    Mikrobiologie, Institut Biologie II, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 188:6460-8. 2006
  8. pmc Aerobic benzoyl-coenzyme A (CoA) catabolic pathway in Azoarcus evansii: conversion of ring cleavage product by 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
    Johannes Gescher
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    J Bacteriol 188:2919-27. 2006
  9. pmc Cholest-4-en-3-one-delta 1-dehydrogenase, a flavoprotein catalyzing the second step in anoxic cholesterol metabolism
    Yin Ru Chiang
    Mikrobiologie, Institut Biologie II, Albert Ludwigs Universitat Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    Appl Environ Microbiol 74:107-13. 2008
  10. pmc Anaerobic metabolism of catechol by the denitrifying bacterium Thauera aromatica--a result of promiscuous enzymes and regulators?
    Bin Ding
    Mikrobiologie, Fakultat fur Biologie, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 190:1620-30. 2008

Collaborators

Detail Information

Publications69

  1. doi request reprint Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota
    Ivan A Berg
    Mikrobiologie, Fakultat Biologie, Albert Ludwigs Universitat Freiburg, Germany
    Microbiology 156:256-69. 2010
    ..We conclude that autotrophic Crenarchaeota use one of the two cycles, and that their distribution correlates with the 16S rRNA-based phylogeny of this group, rather than with the aerobic or anaerobic lifestyle...
  2. ncbi request reprint New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii
    Annette Zaar
    Institut fur Biologie II, Mikrobiologie, Universitat Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    Mol Microbiol 54:223-38. 2004
    ..The benzoyl-CoA oxygenase system has very low similarity to known (di)oxygenase systems and is the first member of a new enzyme family...
  3. doi request reprint Anaerobic metabolism of aromatic compounds
    Georg Fuchs
    Microbiology, Faculty of Biology, University of Freiburg, Schaenzelstr 1, D 79104 Freiburg, Germany
    Ann N Y Acad Sci 1125:82-99. 2008
    ..4) A completely different, still little characterized benzoyl-coenzyme A reductase operates in strict anaerobes, which cannot afford the costly ATP-dependent ring reduction...
  4. doi request reprint Microbial degradation of aromatic compounds - from one strategy to four
    Georg Fuchs
    Mikrobiologie, Universitat Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    Nat Rev Microbiol 9:803-16. 2011
    ....
  5. doi request reprint Alternative pathways of carbon dioxide fixation: insights into the early evolution of life?
    Georg Fuchs
    Lehrstuhl Mikrobiologie, Universitat Freiburg, D 79104 Freiburg, Germany
    Annu Rev Microbiol 65:631-58. 2011
    ..It appears that the formation of an activated acetic acid from inorganic carbon represents the initial step toward metabolism. Consequently, biosyntheses likely started from activated acetic acid and gluconeogenesis preceded glycolysis...
  6. pmc Labeling and enzyme studies of the central carbon metabolism in Metallosphaera sedula
    Sebastian Estelmann
    Mikrobiologie, Fakultat fur Biologie, Freiburg, Germany
    J Bacteriol 193:1191-200. 2011
    ..This study completes the picture of the central carbon metabolism in autotrophic Sulfolobales by connecting the autotrophic carbon fixation cycle to the formation of central carbon precursor metabolites...
  7. pmc Properties of succinyl-coenzyme A:D-citramalate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus
    Silke Friedmann
    Mikrobiologie, Institut Biologie II, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 188:6460-8. 2006
    ..It is proposed that the CoA transferase and the lyase catalyze the last two steps in the glyoxylate assimilation route...
  8. pmc Aerobic benzoyl-coenzyme A (CoA) catabolic pathway in Azoarcus evansii: conversion of ring cleavage product by 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
    Johannes Gescher
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    J Bacteriol 188:2919-27. 2006
    ..The further metabolism is thought to lead to 3-oxoadipyl-CoA, the intermediate at which the conventional and the unorthodox pathways merge...
  9. pmc Cholest-4-en-3-one-delta 1-dehydrogenase, a flavoprotein catalyzing the second step in anoxic cholesterol metabolism
    Yin Ru Chiang
    Mikrobiologie, Institut Biologie II, Albert Ludwigs Universitat Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    Appl Environ Microbiol 74:107-13. 2008
    ..Due to its catalytic properties, the enzyme may be useful in steroid transformations...
  10. pmc Anaerobic metabolism of catechol by the denitrifying bacterium Thauera aromatica--a result of promiscuous enzymes and regulators?
    Bin Ding
    Mikrobiologie, Fakultat fur Biologie, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 190:1620-30. 2008
    ..Hence, the promiscuity of several enzymes and regulatory proteins may be sufficient to create the catechol pathway that is made up of elements of phenol, 3-hydroxybenzoate, 4-hydroxybenzoate, and benzoate metabolism...
  11. pmc Mesaconyl-coenzyme A hydratase, a new enzyme of two central carbon metabolic pathways in bacteria
    Jan Zarzycki
    Mikrobiologie, Fakultat fur Biologie, Universitat Freiburg, Germany
    J Bacteriol 190:1366-74. 2008
    ..A similar yet distinct class of enzymes containing only one hydratase domain was found in various other bacteria, such as Streptomyces species. The role of this widely distributed new enzyme is discussed...
  12. pmc L-malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium Rhodobacter capsulatus
    Michael Meister
    Mikrobiologie, Institut Biologie II, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 187:1415-25. 2005
    ..We therefore propose that L-malyl-CoA/beta-methylmalyl-CoA lyase encoded by mcl1 is involved in acetate assimilation by R. capsulatus and possibly other glyoxylate cycle-negative bacteria...
  13. ncbi request reprint A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea
    Ivan A Berg
    Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Science 318:1782-6. 2007
    ..This indicates the importance of this enzyme in global carbon cycling...
  14. doi request reprint Ethylmalonyl-CoA mutase from Rhodobacter sphaeroides defines a new subclade of coenzyme B12-dependent acyl-CoA mutases
    Tobias J Erb
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg im Breisgau, Germany
    J Biol Chem 283:32283-93. 2008
    ..In combination with molecular modeling, two signature sequences were identified that presumably contribute to the substrate specificity of these enzymes...
  15. doi request reprint Autotrophic carbon fixation in archaea
    Ivan A Berg
    Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Nat Rev Microbiol 8:447-60. 2010
    ..These reactions differ fundamentally from those of the well-known Calvin cycle, and their distribution mirrors the phylogenetic positions of the archaeal lineages and the needs of the ecological niches that they occupy...
  16. ncbi request reprint Aerobic benzoyl-CoA catabolic pathway in Azoarcus evansii: studies on the non-oxygenolytic ring cleavage enzyme
    Johannes Gescher
    Institut fur Biologie II, Mikrobiologie, Fakultat fur Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Mol Microbiol 56:1586-600. 2005
    ..The complex reaction is part of a widely distributed new principle of aerobic aromatic metabolism in which all intermediates are coenzyme A thioesters and the actual ring-cleavage reaction does not require molecular oxygen...
  17. pmc Coenzyme A-dependent aerobic metabolism of benzoate via epoxide formation
    Liv J Rather
    Lehrstuhl Mikrobiologie, Fakultat Biologie, Schanzlestrasse 1, Universitat Freiburg, D 79104 Freiburg, Germany
    J Biol Chem 285:20615-24. 2010
    ..This overlooked pathway occurs in 4-5% of all bacteria whose genomes have been sequenced and represents an elegant strategy to cope with the high resonance energy of aromatic substrates by forming a nonaromatic epoxide...
  18. ncbi request reprint Functional genomics by NMR spectroscopy. Phenylacetate catabolism in Escherichia coli
    Wael Ismail
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Germany
    Eur J Biochem 270:3047-54. 2003
    ..The subsequent beta-oxidation-type degradation of the resulting CoA dicarboxylate via beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA appears to be catalyzed by the PaaJ, PaaF and PaaH proteins...
  19. pmc Phenylphosphate carboxylase: a new C-C lyase involved in anaerobic phenol metabolism in Thauera aromatica
    Karola Schühle
    Mikrobiologie, Institut Biologie II, Universitat Freiburg, Germany
    J Bacteriol 186:4556-67. 2004
    ....
  20. pmc Study of anoxic and oxic cholesterol metabolism by Sterolibacterium denitrificans
    Yin Ru Chiang
    Mikrobiologie, Fakultat Biologie, Albert Ludwigs Universitat Freiburg, Freiburg, Germany
    J Bacteriol 190:905-14. 2008
    ..Hence, this facultative anaerobic bacterium may use the anoxic pathway lacking any oxygenase-dependent reaction also under oxic conditions. This confers metabolic flexibility to such facultative anaerobic bacteria...
  21. pmc Benzoate-coenzyme A ligase from Thauera aromatica: an enzyme acting in anaerobic and aerobic pathways
    Karola Schühle
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Germany
    J Bacteriol 185:4920-9. 2003
    ..The product of benzoate activation, benzoyl-CoA, then acts as inducer of separate anaerobic or aerobic pathways of benzoyl-CoA, depending on whether oxygen is lacking or present...
  22. pmc Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus
    Silke Friedmann
    Mikrobiologie, Institut Biologie II, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 189:2906-14. 2007
    ..Incomplete sets of genes were found in aerobic phototrophic bacteria and in the gamma-proteobacterium Congregibacter litoralis. This may indicate that part of the reactions may be involved in a different metabolic process...
  23. pmc Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus
    Jan Zarzycki
    Mikrobiologie, Fakultat fur Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Proc Natl Acad Sci U S A 106:21317-22. 2009
    ..Elements of the 3-hydroxypropionate cycle may be used for the assimilation of small organic molecules. The 3-hydroxypropionate cycle is compared with the Calvin-Benson-Bassham cycle and other autotrophic pathways...
  24. pmc Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales
    Daniel Kockelkorn
    Mikrobiologie, Fakultat Biologie, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 191:6352-62. 2009
    ..Only distantly related genes were found in the genomes of autotrophic marine Crenarchaeota that may use a similar cycle in autotrophic carbon fixation...
  25. pmc Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway
    Tobias J Erb
    Mikrobiologie, Institut fur Biologie II, Fakultat fur Chemie, Pharmazie und Geowissenschaften, Albert Ludwigs Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg, Germany
    Proc Natl Acad Sci U S A 104:10631-6. 2007
    ..For methylotrophic bacteria such as Methylobacterium extorquens, extension of the serine cycle with reactions of the ethylmalonyl-CoA pathway leads to a simplified scheme for isocitrate lyase-independent C1 assimilation...
  26. pmc 3-Hydroxypropionyl-coenzyme A synthetase from Metallosphaera sedula, an enzyme involved in autotrophic CO2 fixation
    Birgit E Alber
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Germany
    J Bacteriol 190:1383-9. 2008
    ..sedula, supporting its proposed role during CO2 fixation in this archaeon and possibly other members of the Sulfolobaceae family...
  27. pmc 3-hydroxypropionyl-coenzyme A dehydratase and acryloyl-coenzyme A reductase, enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in the Sulfolobales
    Robin Teufel
    Mikrobiologie, Fakultat Biologie, Freiburg, Germany
    J Bacteriol 191:4572-81. 2009
    ..This indicates that the autotrophic carbon fixation cycles in Chloroflexus and in the Sulfolobales evolved independently and that different genes/enzymes have been recruited in the two lineages that catalyze the same kinds of reactions...
  28. pmc Phosphorylation of phenol by phenylphosphate synthase: role of histidine phosphate in catalysis
    Ariun Narmandakh
    Department Mikrobiologie, Institut Biologie II, Albert Ludwigs Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    J Bacteriol 188:7815-22. 2006
    ..First, a diphosphoryl group is transferred to His-569 in protein 1, from which phosphate is cleaved to render the reaction unidirectional. Histidine phosphate subsequently serves as the actual phosphorylation agent...
  29. pmc Phenylphosphate synthase: a new phosphotransferase catalyzing the first step in anaerobic phenol metabolism in Thauera aromatica
    Sirko Schmeling
    Institut Biologie II, Mikrobiologie, Albert Ludwigs Universitat Freiburg, D 79104 Freiburg, Germany
    J Bacteriol 186:8044-57. 2004
    ..The molecular and catalytic features of phenylphosphate synthase resemble those of phosphoenolpyruvate synthase, albeit with interesting modifications...
  30. ncbi request reprint Assaying for the 3-hydroxypropionate cycle of carbon fixation
    Michael Hügler
    Faculty of Biology, University of Freiburg, Germany
    Methods Enzymol 397:212-21. 2005
    ..The fate of succinyl-CoA in Sulfolobales is at issue. Assays used to study the characteristic enzymes of this novel pathway in C. aurantiacus are reported...
  31. doi request reprint The reducing component BoxA of benzoyl-coenzyme A epoxidase from Azoarcus evansii is a [4Fe-4S] protein
    Liv J Rather
    Mikrobiologie, Fakultat Biologie, Schanzlestrasse 1, Universitat Freiburg, Freiburg, Germany
    Biochim Biophys Acta 1814:1609-15. 2011
    ..In other cases within the catalytically versatile family of diiron enzymes, the related reductases have plant-type ferredoxin or Rieske-type [2Fe-2S] centers only...
  32. ncbi request reprint Characterization of acetyl-CoA/propionyl-CoA carboxylase in Metallosphaera sedula. Carboxylating enzyme in the 3-hydroxypropionate cycle for autotrophic carbon fixation
    Michael Hügler
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Germany
    Eur J Biochem 270:736-44. 2003
    ..Due to its stability the archaeal carboxylase may prove an ideal subject for further structural studies...
  33. ncbi request reprint Study of an alternate glyoxylate cycle for acetate assimilation by Rhodobacter sphaeroides
    Birgit E Alber
    Mikrobiologie, Fakultat fur Biologie, Albert Ludwigs Universitat Freiburg, Germany
    Mol Microbiol 61:297-309. 2006
    ..In a second part glyoxylate and propionyl-CoA are converted with another molecule of acetyl-CoA and CO(2) to l-malyl-CoA and succinyl-CoA...
  34. doi request reprint Phenylacetate metabolism in thermophiles: characterization of phenylacetate-CoA ligase, the initial enzyme of the hybrid pathway in Thermus thermophilus
    Tobias J Erb
    Institut für Biologie II Mikrobiologie, Albert Ludwigs Universitat Freiburg, Freiburg, Germany
    Curr Microbiol 57:27-32. 2008
    ..Enzymes of this thermophile are expected to be robust and might be useful for further studies of this yet unresolved pathway...
  35. pmc Malonyl-coenzyme A reductase in the modified 3-hydroxypropionate cycle for autotrophic carbon fixation in archaeal Metallosphaera and Sulfolobus spp
    Birgit Alber
    Mikrobiologie, Institut Biologie II, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 188:8551-9. 2006
    ..This shows that the autotrophic pathway in Chloroflexus and Sulfolobaceae has evolved convergently and that these taxonomic groups have recruited different genes to bring about similar metabolic processes...
  36. doi request reprint (2S)-Methylsuccinyl-CoA dehydrogenase closes the ethylmalonyl-CoA pathway for acetyl-CoA assimilation
    Tobias J Erb
    Mikrobiologie, Institut fur Biologie II, Schänzlestr 1, Albert Ludwigs Universitat Freiburg, Freiburg i Br, Germany
    Mol Microbiol 73:992-1008. 2009
    ..This allowed defining the minimum set of enzymes necessary for its operation and to screen for further organisms following this acetyl-CoA assimilation strategy...
  37. ncbi request reprint Alicycliphilus denitrificans gen. nov., sp. nov., a cyclohexanol-degrading, nitrate-reducing beta-proteobacterium
    Tahar Mechichi
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    Int J Syst Evol Microbiol 53:147-52. 2003
    ..Based on the high 16S rDNA sequence divergence and phenotypic characteristics, the name Alicycliphilus denitrificans gen. nov., sp. nov. is proposed for this strain. The type strain is K60IT (=DSM 14773T =CIP 107495T)...
  38. pmc Molybdoenzyme that catalyzes the anaerobic hydroxylation of a tertiary carbon atom in the side chain of cholesterol
    Juri Dermer
    Lehrstuhl Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    J Biol Chem 287:36905-16. 2012
    ..The draft genome of the organism contains several genes coding for related enzymes that probably replace oxygenases in steroid metabolism...
  39. pmc Anaerobic metabolism of indoleacetate
    Christa Ebenau-Jehle
    Mikrobiologie, Fakultat fur Biologie, Albert Ludwigs Universitat Freiburg, Freiburg, Germany
    J Bacteriol 194:2894-903. 2012
    ..We propose a scheme of an anaerobic IAA metabolic pathway that ultimately leads to 2-aminobenzoyl-CoA or benzoyl-CoA...
  40. pmc Autotrophic carbon dioxide assimilation in Thermoproteales revisited
    W Hugo Ramos-Vera
    Mikrobiologie, Fakultat Biologie, Albert Ludwigs Universitat Freiburg, Freiburg, Germany
    J Bacteriol 191:4286-97. 2009
    ..We conclude that the dicarboxylate/4-hydroxybutyrate cycle is operating in CO(2) fixation in the strict anaerobic Thermoproteales as well as in Desulfurococcales...
  41. pmc Structure and mechanism of the diiron benzoyl-coenzyme A epoxidase BoxB
    Liv J Rather
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    J Biol Chem 286:29241-8. 2011
    ..We postulate a reaction cycle that allows a charge delocalization because of the phenyl ring and the electron-withdrawing CoA thioester...
  42. pmc Studies on the mechanism of ring hydrolysis in phenylacetate degradation: a metabolic branching point
    Robin Teufel
    Lehrstuhl Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Freiburg, Germany
    J Biol Chem 286:11021-34. 2011
    ..Apparently, bacteria made a virtue out of the necessity of disposing the dead-end product with ring hydrolysis as a metabolic branching point...
  43. pmc Properties of succinyl-coenzyme A:L-malate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus
    Silke Friedmann
    Mikrobiologie, Institut Biologie II, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 188:2646-55. 2006
    ..The enzyme is a member of the class III of the CoA transferase family. The demonstration of the missing CoA transferase closes the last gap in the proposed 3-hydroxypropionate cycle...
  44. doi request reprint Carbon dioxide fixation in 'Archaeoglobus lithotrophicus': are there multiple autotrophic pathways?
    Sebastian Estelmann
    Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Freiburg, Germany
    FEMS Microbiol Lett 319:65-72. 2011
    ..Our data indicate that the reductive acetyl-CoA pathway is the only functioning CO(2) fixation pathway in 'A. lithotrophicus'...
  45. pmc Genes coding for a new pathway of aerobic benzoate metabolism in Azoarcus evansii
    Johannes Gescher
    Mikrobiologie, Institut Biologie II, Universitat Freiburg, Germany
    J Bacteriol 184:6301-15. 2002
    ..A beta-oxidation-like metabolism of the ring cleavage product is thought to lead to 3-ketoadipyl-CoA, which finally is cleaved into succinyl-CoA and acetyl-CoA...
  46. pmc 2-Oxoglutarate:NADP(+) oxidoreductase in Azoarcus evansii: properties and function in electron transfer reactions in aromatic ring reduction
    Christa Ebenau-Jehle
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Freiburg, Germany
    J Bacteriol 185:6119-29. 2003
    ..A similar NADP(+)-specific pyruvate oxidoreductase, which contains all domains in one large subunit, has been reported for the mitochondrion of the protist Euglena gracilis and the apicomplexan Cryptosporidium parvum...
  47. pmc Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase
    Tobias J Erb
    Mikrobiologie, Institut fur Biologie II, Schanzlestrasse 1, Albertstrasse 25, Albert Ludwigs Universitat Freiburg, 79104 Freiburg im Breisgau, Germany
    Proc Natl Acad Sci U S A 106:8871-6. 2009
    ..This may be useful for protein engineering of enoyl reductases and their application in biocatalysis...
  48. ncbi request reprint Anaerobic oxidation of aromatic compounds and hydrocarbons
    Matthias Boll
    Institut fur Biologie II, Mikrobiologie, Schänzlestr 1, D 79104 Freiburg, Germany
    Curr Opin Chem Biol 6:604-11. 2002
    ..These reactions transform aromatic rings and hydrocarbons into products that can be oxidized via more conventional beta-oxidation pathways...
  49. doi request reprint An oxygenase that forms and deoxygenates toxic epoxide
    Robin Teufel
    Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Nature 483:359-62. 2012
    ..Hence, PaaABCE may harbour an intrinsic escape mechanism from its own toxic product and represents the archetype of a bifunctional oxygenase/deoxygenase. Analogous reactions may possibly be catalysed by other di-iron epoxidases...
  50. doi request reprint A spectrophotometric assay for measuring acetyl-coenzyme A carboxylase
    Jasmin K Kroeger
    Mikrobiologie, Fakultat fur Biologie, Universitat Freiburg, D 79104 Freiburg, Germany
    Anal Biochem 411:100-5. 2011
    ..This assay can be adapted for high-throughput screening...
  51. doi request reprint Anaerobic metabolism of phenol in proteobacteria and further studies of phenylphosphate carboxylase
    Sirko Schmeling
    Mikrobiologie, Fakultat fur Biologie, Universitat Freiburg, Schänzlestr 1, 79104, Freiburg, Germany
    Arch Microbiol 191:869-78. 2009
    ....
  52. ncbi request reprint Aerobic metabolism of phenylacetic acids in Azoarcus evansii
    Magdy el Said Mohamed
    Mikrobiologie, Institut fur Biologie II, Schanzlestrasse 1, Universitat Freiburg, 79104 Freiburg, Germany
    Arch Microbiol 178:180-92. 2002
    ..evansiigrowing aerobically with PA showed a probable organization of the paa genes in three operons. The similarity of the PA metabolic products pattern and of gene sequences suggests a common aerobic bacterial PA pathway...
  53. pmc Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota
    W Hugo Ramos-Vera
    Mikrobiologie, Fakultat Biologie, Freiburg, Germany
    J Bacteriol 193:1201-11. 2011
    ..In contrast, several of the other enzymes evolved from quite different sources, making functional predictions based solely on genome interpretation difficult, if not questionable...
  54. doi request reprint Fructose 1,6-bisphosphate aldolase/phosphatase may be an ancestral gluconeogenic enzyme
    Rafael F Say
    Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Nature 464:1077-81. 2010
    ..We propose that this highly conserved, heat-stabile and bifunctional FBP aldolase/phosphatase represents the pace-making ancestral gluconeogenic enzyme, and that in evolution gluconeogenesis preceded glycolysis...
  55. pmc Regulation of autotrophic CO2 fixation in the archaeon Thermoproteus neutrophilus
    W Hugo Ramos-Vera
    Mikrobiologie, Fakultat Biologie, Albert Ludwigs Universitat Freiburg, Freiburg, Germany
    J Bacteriol 192:5329-40. 2010
    ..This system may be suitable for molecular analysis of the transcriptional regulation of autotrophy-related genes...
  56. ncbi request reprint Initial steps in the anoxic metabolism of cholesterol by the denitrifying Sterolibacterium denitrificans
    Yin Ru Chiang
    Mikrobiologie, Fakultat fur Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    J Biol Chem 282:13240-9. 2007
    ..The anaerobic hydroxylation of a tertiary carbon using water as oxygen donor is unprecedented and may be catalyzed by a novel molybdenum containing enzyme...
  57. pmc L-Malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus, a bifunctional enzyme involved in autotrophic CO(2) fixation
    Sylvia Herter
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Germany
    J Bacteriol 184:5999-6006. 2002
    ..In summary, L-malyl-CoA lyase/beta-methylmalyl-CoA lyase catalyzes the interconversion of L-malyl-CoA plus propionyl-CoA to beta-methylmalyl-CoA plus acetyl-CoA...
  58. pmc Coassimilation of organic substrates via the autotrophic 3-hydroxypropionate bi-cycle in Chloroflexus aurantiacus
    Jan Zarzycki
    Mikrobiologie, Fakultat fur Biologie, Schänzlestraße 1, D 79104 Freiburg, Germany
    Appl Environ Microbiol 77:6181-8. 2011
    ..Examples are Chloroherpeton thalassium, Erythrobacter sp. strain NAP-1, Nitrococcus mobilis, and marine gammaproteobacteria of the OM60/NOR5 clade such as Congregibacter litoralis...
  59. ncbi request reprint Propionyl-coenzyme A synthase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO2 fixation
    Birgit E Alber
    Institut Biologie II, Mikrobiologie, Universitat Freiburg, Schanzlestrasse 1, Freiburg D 79104, Germany
    J Biol Chem 277:12137-43. 2002
    ..g. polyketide synthases) but rare in primary metabolism (e.g. eukaryotic type I fatty acid synthase). These results lend strong support to the operation of the proposed pathway in autotrophic CO(2) fixation...
  60. pmc Malonyl-coenzyme A reductase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO(2) fixation
    Michael Hügler
    Mikrobiologie, Institut fur Biologie II, Albert Ludwigs Universitat Freiburg, Freiburg, Germany
    J Bacteriol 184:2404-10. 2002
    ..Furthermore, the enzyme may be useful for production of 3-hydroxypropionate and for a coupled spectrophotometric assay for activity screening of acetyl-CoA carboxylase, a target enzyme of potent herbicides...
  61. ncbi request reprint Autotrophic CO2 fixation pathways in archaea (Crenarchaeota)
    Michael Hügler
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Schanzlestrasse 1, Germany
    Arch Microbiol 179:160-73. 2003
    ..We conclude that Crenarchaeota exhibit a mosaic of three or possibly four autotrophic pathways. The distribution of the pathways so far correlates with the 16S-rRNA-based taxa of the Crenarchaeota...
  62. ncbi request reprint A new purple sulfur bacterium isolated from a littoral microbial mat, Thiorhodococcus drewsii sp. nov
    Annette Zaar
    Mikrobiologie, Institut fur Biologie II, Universtität Freiburg, Schanzlestrasse 1, Germany
    Arch Microbiol 179:174-83. 2003
    ..minor, the new isolate is described as a new species of the genus Thiorhodococcus, Thiorhodococcus drewsii sp. nov...
  63. ncbi request reprint Unusual reactions involved in anaerobic metabolism of phenolic compounds
    Matthias Boll
    Department of Microbiology, Institute for Biology II, University of Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    Biol Chem 386:989-97. 2005
    ..This review summarizes the results of recent mechanistic studies of the enzymes involved in these three key reactions...
  64. ncbi request reprint Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp. nov., Thauera aminoaromaticasp. nov., and Azoarcus buckelii sp. nov
    Tahar Mechichi
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg, Germany
    Arch Microbiol 178:26-35. 2002
    ....
  65. pmc The bzd gene cluster, coding for anaerobic benzoate catabolism, in Azoarcus sp. strain CIB
    María J López Barragán
    Departamento de Microbiologia Molecular, Centro de Investigaciones Biologicas CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain
    J Bacteriol 186:5762-74. 2004
    ..strain CIB is subject to carbon catabolite repression by some organic acids, indicating the existence of a physiological control that connects the expression of the bzd genes to the metabolic status of the cell...
  66. pmc Evidence for autotrophic CO2 fixation via the reductive tricarboxylic acid cycle by members of the epsilon subdivision of proteobacteria
    Michael Hügler
    Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA
    J Bacteriol 187:3020-7. 2005
    ....
  67. pmc Insights into the autotrophic CO2 fixation pathway of the archaeon Ignicoccus hospitalis: comprehensive analysis of the central carbon metabolism
    Ulrike Jahn
    Lehrstuhl Mikrobiologie und Archaeenzentrum, Universitat Regensburg, Universitatsstrasse 31, D 93053 Regensburg, Germany
    J Bacteriol 189:4108-19. 2007
    ..This comprehensive analysis of the central carbon metabolism of I. hospitalis revealed further evidence for the unexpected and unexplored diversity of metabolic pathways within the (hyperthermophilic) archaea...
  68. ncbi request reprint A bicyclic autotrophic CO2 fixation pathway in Chloroflexus aurantiacus
    Sylvia Herter
    Lehrstuhl fur Mikrobiologie, Institut Biologie II, Universitat Freiburg, Schänzlestr 1, D 79104 Freiburg, Germany
    J Biol Chem 277:20277-83. 2002
    ..The net product of that bicyclic autotrophic CO(2) fixation pathway is pyruvate serving as an universal building block for anabolic reactions...
  69. pmc A dicarboxylate/4-hydroxybutyrate autotrophic carbon assimilation cycle in the hyperthermophilic Archaeum Ignicoccus hospitalis
    Harald Huber
    Lehrstuhl fur Mikrobiologie und Archaeenzentrum, Universitat Regensburg, Universitaetsstrasse 31, D 93053 Regensburg, Germany
    Proc Natl Acad Sci U S A 105:7851-6. 2008
    ..The pathway is termed the dicarboxylate/4-hydroxybutyrate cycle. It combines anaerobic metabolic modules to a straightforward and efficient CO(2) fixation mechanism...