W A Flegel

Summary

Affiliation: University of Ulm
Country: Germany

Publications

  1. ncbi request reprint Molecular genetics of RH and its clinical application
    W A Flegel
    Institut fur klinische Transfusionsmedizin und Immungenetik Ulm, Abteilung Transfusionsmedizin, Universitatsklinikum Ulm, Ulm, Germany
    Transfus Clin Biol 13:4-12. 2006
  2. ncbi request reprint Molecular biology of partial D and weak D: implications for blood bank practice
    Willy A Flegel
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm and DRK Blutspendedienst Baden Württemberg, Institut Ulm, Germany
    Clin Lab 48:53-9. 2002
  3. ncbi request reprint In-frame triplet deletions in RHD alter the D antigen phenotype
    Willy A Flegel
    Department of Transfusion Medicine, University Hospital, Ulm, Germany
    Transfusion 46:2156-61. 2006
  4. doi request reprint Six years' experience performing RHD genotyping to confirm D- red blood cell units in Germany for preventing anti-D immunizations
    Willy A Flegel
    German Red Cross DRK Blood Donor Service Baden Württemberg Hessen, Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, Ulm, Germany
    Transfusion 49:465-71. 2009
  5. doi request reprint D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters
    Willy A Flegel
    Institute for Transfusion Medicine, University Hospital, Ulm, Germany
    Transfusion 49:1059-69. 2009
  6. ncbi request reprint How I manage donors and patients with a weak D phenotype
    Willy A Flegel
    Institute for Clinical Transfusion Medicine and Immunogenetics, Ulm, Germany
    Curr Opin Hematol 13:476-83. 2006
  7. ncbi request reprint Blood group genotyping in Germany
    Willy A Flegel
    Institute of Transfusion Medicine, University Hospital Ulm, Germany
    Transfusion 47:47S-53S. 2007
  8. ncbi request reprint DCS-1, DCS-2, and DFV share amino acid substitutions at the extracellular RhD protein vestibule
    Willy A Flegel
    Institute for Transfusion Medicine, University Hospital Ulm, Ulm Germany
    Transfusion 48:25-33. 2008
  9. ncbi request reprint Molecular basis of weak D phenotypes
    F F Wagner
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm and DRK Blutspendedienst Baden Württemberg, Institut Ulm, Ulm, Germany
    Blood 93:385-93. 1999
  10. pmc RHD positive haplotypes in D negative Europeans
    F F Wagner
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm and DRK Blutspendedienst Baden Württemberg, Institut Ulm, Ulm, Germany
    BMC Genet 2:10. 2001

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Molecular genetics of RH and its clinical application
    W A Flegel
    Institut fur klinische Transfusionsmedizin und Immungenetik Ulm, Abteilung Transfusionsmedizin, Universitatsklinikum Ulm, Ulm, Germany
    Transfus Clin Biol 13:4-12. 2006
    ..In the last five years the field has been moving from defining the underlying molecular genetics to applying the molecular genetics in clinical practice...
  2. ncbi request reprint Molecular biology of partial D and weak D: implications for blood bank practice
    Willy A Flegel
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm and DRK Blutspendedienst Baden Württemberg, Institut Ulm, Germany
    Clin Lab 48:53-9. 2002
    ..Aberrant RHD alleles encode partial D, some of which were dubbed D categories, and weak D. Since we defined the molecular basis of the RHD deletion, a specific detection of heterozygous carriers became feasible...
  3. ncbi request reprint In-frame triplet deletions in RHD alter the D antigen phenotype
    Willy A Flegel
    Department of Transfusion Medicine, University Hospital, Ulm, Germany
    Transfusion 46:2156-61. 2006
    ..Only one such in-frame deletion is known in the two RH genes, represented by the RHCE allele ceBP expressing a "very weak e antigen."..
  4. doi request reprint Six years' experience performing RHD genotyping to confirm D- red blood cell units in Germany for preventing anti-D immunizations
    Willy A Flegel
    German Red Cross DRK Blood Donor Service Baden Württemberg Hessen, Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, Ulm, Germany
    Transfusion 49:465-71. 2009
    ..Red blood cell (RBC) units of D+ donors are falsely labeled D- if regular serologic typing fails to detect low D antigen expression or chimerism. The limitations of serology can be overcome by molecular typing...
  5. doi request reprint D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters
    Willy A Flegel
    Institute for Transfusion Medicine, University Hospital, Ulm, Germany
    Transfusion 49:1059-69. 2009
    ..F223V as well as a large number of further substitutions causing D variants are located at the extracellular RhD protein vestibule, which represents the entrance to the transmembraneous channel of the RhD protein...
  6. ncbi request reprint How I manage donors and patients with a weak D phenotype
    Willy A Flegel
    Institute for Clinical Transfusion Medicine and Immunogenetics, Ulm, Germany
    Curr Opin Hematol 13:476-83. 2006
    ..I offer an approach to the management of donors and patients expressing D antigen weakly and carrying any of the various molecular types of weak D and DEL...
  7. ncbi request reprint Blood group genotyping in Germany
    Willy A Flegel
    Institute of Transfusion Medicine, University Hospital Ulm, Germany
    Transfusion 47:47S-53S. 2007
    ..Since then, the clinical applications have been expanded and refined. Their implementation varies considerably among different health-care systems, notably between North America and Europe...
  8. ncbi request reprint DCS-1, DCS-2, and DFV share amino acid substitutions at the extracellular RhD protein vestibule
    Willy A Flegel
    Institute for Transfusion Medicine, University Hospital Ulm, Ulm Germany
    Transfusion 48:25-33. 2008
    ..Three RhD variants with amino acid substitutions located at the extracellular channel aperture are described...
  9. ncbi request reprint Molecular basis of weak D phenotypes
    F F Wagner
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm and DRK Blutspendedienst Baden Württemberg, Institut Ulm, Ulm, Germany
    Blood 93:385-93. 1999
    ..Our results showed means to specifically detect and to classify weak D. The genotyping of weak D may guide Rhesus negative transfusion policy for such molecular weak D types that were prone to develop anti-D...
  10. pmc RHD positive haplotypes in D negative Europeans
    F F Wagner
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm and DRK Blutspendedienst Baden Württemberg, Institut Ulm, Ulm, Germany
    BMC Genet 2:10. 2001
    ..Excluding RHDpsi and CdeS frequent only in individuals of African descent, most of these alleles are unknown and the population frequency of any such allele has not been determined...
  11. ncbi request reprint Random survey for RHD alleles among D+ European persons
    Qing Chen
    Department of Transfusion Medicine, University Hospital Ulm, Ulm, Germany
    Transfusion 45:1183-91. 2005
    ..RHD alleles are considered more variable in African persons than in European persons. A systematic survey, however, was lacking among D+ European persons at the molecular level, precluding any definite frequency estimate...
  12. ncbi request reprint Three molecular structures cause rhesus D category VI phenotypes with distinct immunohematologic features
    F F Wagner
    Abteilung Transfusionsmedizin, Universitat Ulm and DRK Blutspendezentrale Ulm, Ulm, Germany
    Blood 91:2157-68. 1998
    ..Genotyping strategies should take account of allelic variations in partial RhD. The reconsideration of previous serologic and clinical data for partial D in view of the underlying molecular structures may be worthwhile...
  13. ncbi request reprint Rh phenotype prediction by DNA typing and its application to practice
    W A Flegel
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm, Germany
    Transfus Med 8:281-302. 1998
    ..Transfusion medicine is in the unique position of being able to utilize the most extensive phenotype databases available to check and develop genotyping strategies...
  14. ncbi request reprint RHD gene deletion occurred in the Rhesus box
    F F Wagner
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm, Ulm, Germany
    Blood 95:3662-8. 2000
    ..The molecular structure of the RH gene locus explains the mechanisms for generating RHD/RHCE hybrid alleles and the RHD deletion. Specific detection of the RHD(-) genotype is now possible. (Blood. 2000;95:3662-3668)..
  15. ncbi request reprint The DAU allele cluster of the RHD gene
    Franz F Wagner
    Abteilung Transfusionsmedizin, Universitätsklinikum Ulm and DRK Blutspendedienst Baden Württemberg Hessen, Institut Ulm, Ulm, Germany
    Blood 100:306-11. 2002
    ..The identification of the DAU alleles increased the number of known partial D alleles in Africans considerably. DAU alleles may be a major cause of antigen D variability and anti-D immunization in patients of African descent...
  16. ncbi request reprint The RHCE allele ceCF: the molecular basis of Crawford (RH43)
    Willy A Flegel
    Department of Transfusion Medicine, University Hospital, Ulm, Germany
    Transfusion 46:1334-42. 2006
    ..The Crawford antigen (RH43) was described in 1980. It occurred in African American people, as a low-prevalence Rhesus antigen, who were also VS+...
  17. ncbi request reprint The RHCE allele ceSL: the second example for D antigen expression without D-specific amino acids
    Qing Chen
    Department of Transfusion Medicine, University Hospital, Ulm, Germany
    Transfusion 46:766-72. 2006
    ..No other example exposing a similar molecular mechanism was known...
  18. ncbi request reprint The RHCE allele ceRT: D epitope 6 expression does not require D-specific amino acids
    Franz F Wagner
    Department of Transfusion Medicine, University of Ulm, Ulm, Germany
    Transfusion 43:1248-54. 2003
    ..Known causes of such errors are RhCE variants carrying RhD-specific amino acids and cold agglutinin activity of some frequently used monoclonal anti-D...
  19. ncbi request reprint Weak D alleles express distinct phenotypes
    F F Wagner
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm and DRK Blutspendedienst Baden Württemberg, Institut Ulm, Ulm, Germany
    Blood 95:2699-708. 2000
    ..Blood. 2000;95:2699-2708)..
  20. ncbi request reprint Section 1B: Rh flow cytometry. Coordinator's report. Rhesus index and antigen density: an analysis of the reproducibility of flow cytometric determination
    W A Flegel
    Abteilung Transfusionsmedizin, Universitatsklinikum Ulm, DRK Blutspendedienst Baden Württemberg, Institut Ulm, Ulm, Germany
    Transfus Clin Biol 9:33-42. 2002
    ..The antigen densities and the Rhesus indices obtained correlated well among the laboratories of this Workshop section despite different staining protocols, secondary antibodies and instrumentation...
  21. ncbi request reprint An easy RHD genotyping strategy for D- East Asian persons applied to Korean blood donors
    Timo A Luettringhaus
    Department of Transfusion Medicine, University Hospital, Ulm, Germany
    Transfusion 46:2128-37. 2006
    ..The molecular basis of the "RHD(el)" allele previously reported in Taiwan to harbor a genomic 1013-bp deletion was questioned by several authors...
  22. pmc RHD allele distribution in Africans of Mali
    Franz F Wagner
    Department of Transfusion Medicine, University Hospital, Ulm, Germany
    BMC Genet 4:14. 2003
    ..The DAU cluster of RHD alleles exemplifies that the alleles frequent in Africans have evaded recognition until recently. A comprehensive survey of RHD alleles in any African population was lacking...
  23. ncbi request reprint Genetic mechanisms of Rhesus box variation
    Franz F Wagner
    Department of Transfusion Medicine, University Hospital, Ulm, Germany
    Transfusion 45:338-44. 2005
    ..Aberrant Rhesus boxes can confound this application and appear to be frequent among African individuals...
  24. ncbi request reprint Predicting a donor's likelihood of donating within a preselected time interval
    W A Flegel
    Department of Transfusion Medicine, University of Ulm, DRK German Red Cross Blood Service Baden Württemberg, Institute Ulm, Ulm, Germany
    Transfus Med 10:181-92. 2000
    ..General implications for blood collection, like planning blood drives, were discussed. The whole demand of a health care system for single plasma units may be met by quarantine plasma and their cost-efficiency can be improved...
  25. ncbi request reprint Outliers in RhD membrane integration are explained by variant RH haplotypes
    Xinjian Yu
    Department of Transfusion Medicine, University Hospital, and the Institute for Clinical Transfusion Medicine and Immunogenetics, Ulm, Germany
    Transfusion 46:1343-51. 2006
    ..Unlike most other membrane proteins, the expression of the D antigen is often determined by a single allele, because it occurs frequently in hemizygous form...
  26. ncbi request reprint SCER and SCAN: two novel high-prevalence antigens in the Scianna blood group system
    Willy A Flegel
    Department of Transfusion Medicine, University Hospital Ulm, Germany
    Transfusion 45:1940-4. 2005
    ..Their serum samples contained alloantibodies reactive with all RBCs tested except those of the Sc:-1,-2,-3 phenotype. Cloning of the Scianna gene allowed us to determine the molecular bases of these samples...
  27. ncbi request reprint Tissue distribution of blood group membrane proteins beyond red cells: evidence from cDNA libraries
    Markus T Rojewski
    Department of Transfusion Medicine, University Hospital, Ulm, Germany
    Transfus Apher Sci 35:71-82. 2006
    ..The genes were grouped according to their overall and their relative expression in embryo and adults. We describe the distribution of EST in cells, tissues and cell lines with a focus on non-RBC tissues...
  28. ncbi request reprint On the complexity of D antigen typing: a handy decision tree in the age of molecular blood group diagnostics
    Willy A Flegel
    Institute for Transfusion Medicine, University Hospital Ulm, Germany
    J Obstet Gynaecol Can 29:746-52. 2007
    ..We demonstrate how molecular tests of the RHD gene can and should be used in resolving serological discrepancies, in particular in pregnant women...
  29. pmc Ultrasonographically detected gallbladder polyps: a reason for concern? A seven-year follow-up study
    Wolfgang Kratzer
    Zentrum fur Innere Medizin, Klinik fur Innere Medizin I, Universitatsklinikum Ulm, Robert Koch Str, 8, 89081 Ulm, Germany
    BMC Gastroenterol 8:41. 2008
    ..Objective of the present study was to investigate the prevalence and growth of gallbladder polyps in a survey of unselected subjects from the general population of a complete rural community...
  30. ncbi request reprint DNB: a partial D with anti-D frequent in Central Europe
    Franz F Wagner
    Department of Transfusion Medicine, University of Ulm, DRK German Red Cross Blood Donation Service Baden Württemberg Hessen, Institute Ulm, Heimholtzstrasse 10, D 89081 Ulm, Germany
    Blood 100:2253-6. 2002
    ..DNB was the most frequent partial D recognized so far in whites, occurring with frequencies of up to 1:292 in Switzerland. DNB was the underlying partial D phenotype in a relevant fraction of anti-D immunizations occurring in whites...
  31. ncbi request reprint IVS5-38del4 deletion in the RHD gene does not cause a DEL phenotype: relevance for RHD alleles including DFR-3
    Inge von Zabern
    Transfusion 47:1552-5. 2007
  32. ncbi request reprint The BloodGen project: toward mass-scale comprehensive genotyping of blood donors in the European Union and beyond
    Neil D Avent
    Center for Research in Biomedicine and Bristol Genomics Research Institute, Faculty of Applied Sciences, University of the West of England, and Bristol Institute for Transfusion Sciences, Bristol, UK
    Transfusion 47:40S-6S. 2007
  33. ncbi request reprint Partial D, weak D types, and novel RHD alleles among 33,864 multiethnic patients: implications for anti-D alloimmunization and prevention
    Gregory A Denomme
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada
    Transfusion 45:1554-60. 2005
    ..At present, there is little prospective information on the prevalence of D variants among obstetric patients and potential transfusion recipients...
  34. ncbi request reprint The D category VI type 4 allele is prevalent in the Spanish population
    Rosa Esteban
    Immunohematology Service, Banc de Sang i Teixits, Barcelona, Spain
    Transfusion 46:616-23. 2006
    ..The D category VI (DVI) is one of the clinically most important partial D. Three different molecular structures causing the DVI phenotype have been described...
  35. ncbi request reprint Weak D type 1.1 exemplifies another complexity in weak D genotyping
    Andrea Doescher
    DRK Blutspendedienst NSTOB, Oldenburg Institute, Oldenburg, Germany
    Transfusion 45:1568-73. 2005
    ..Possible sources of error are rare D variants that are inadvertently carrying known polymorphisms of frequent weak D types...
  36. ncbi request reprint RHCE represents the ancestral RH position, while RHD is the duplicated gene
    Franz F Wagner
    Blood 99:2272-3. 2002
  37. ncbi request reprint Homing in on D antigen immunogenicity
    Willy A Flegel
    Transfusion 45:466-8. 2005
  38. ncbi request reprint Scianna antigens including Rd are expressed by ERMAP
    Franz F Wagner
    Department of Transfusion Medicine, University of Ulm, DRK German Red Cross Blood Donation Service Baden Württemberg Hessen, Institute Ulm, Germany
    Blood 101:752-7. 2003
    ..Hence, the phenotype prediction by genotyping became possible for all human blood group systems encoded by proteins...
  39. ncbi request reprint Histo-blood group antigens as allo- and autoantigens
    Urs E Nydegger
    Clinic for Cardiovascular Surgery, Inselspital, CH 3010 Bern, Switzerland
    Ann N Y Acad Sci 1050:40-51. 2005
    ..However, other HBG systems may also play a role, thus far underestimated. This paper is an up-to-date analysis of the importance of HBG systems in the alloimmunity of transplantation and autoimmune events, such as hemolytic anemia...
  40. ncbi request reprint Antibodies to high-frequency antigens may decrease the quality of transfusion support: an observational study
    Axel Seltsam
    Department of Transfusion Medicine, Hannover Medical School, Hannover, Germany
    Transfusion 43:1563-6. 2003
    ..Maintaining a rapidly accessible stock of just four types of rare blood units would ensure adequate transfusion support for most of these patients...