Oliver T Fackler

Summary

Affiliation: University of Heidelberg
Country: Germany

Publications

  1. ncbi request reprint Functional characterization of HIV-1 Nef mutants in the context of viral infection
    Oliver T Fackler
    Department of Virology, University of Heidelberg, INF 324, D 69120 Heidelberg, Germany
    Virology 351:322-39. 2006
  2. ncbi request reprint Modulation of the immunological synapse: a key to HIV-1 pathogenesis?
    Oliver T Fackler
    Oliver T Fackler is at the Department of Virology, University of Heidelberg, D 69120 Heidelberg, Germany
    Nat Rev Immunol 7:310-7. 2007
  3. ncbi request reprint Interactions of human retroviruses with the host cell cytoskeleton
    Oliver T Fackler
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Curr Opin Microbiol 9:409-15. 2006
  4. ncbi request reprint Modulation of specific surface receptors and activation sensitization in primary resting CD4+ T lymphocytes by the Nef protein of HIV-1
    Oliver T Keppler
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Leukoc Biol 79:616-27. 2006
  5. pmc Expression of Nef downregulates CXCR4, the major coreceptor of human immunodeficiency virus, from the surfaces of target cells and thereby enhances resistance to superinfection
    Stephanie Venzke
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Virol 80:11141-52. 2006
  6. ncbi request reprint Specific and distinct determinants mediate membrane binding and lipid raft incorporation of HIV-1(SF2) Nef
    Simone I Giese
    Department of Virology, University of Heidelberg, 69120 Heidelberg, Germany
    Virology 355:175-91. 2006
  7. ncbi request reprint The HIV-1 pathogenicity factor Nef interferes with maturation of stimulatory T-lymphocyte contacts by modulation of N-Wasp activity
    Claudia Haller
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Biol Chem 281:19618-30. 2006
  8. pmc The Nef protein of human immunodeficiency virus is a broad-spectrum modulator of chemokine receptor cell surface levels that acts independently of classical motifs for receptor endocytosis and Galphai signaling
    Nico Michel
    Department of Virology, University of Heidelberg, D 69120 Heidelberg, Germany
    Mol Biol Cell 17:3578-90. 2006
  9. pmc Lentiviral Nef proteins utilize PAK2-mediated deregulation of cofilin as a general strategy to interfere with actin remodeling
    Bettina Stolp
    Department of Infectious Diseases, Virology, University of Heidelberg, INF 324, 69120 Heidelberg, Germany
    J Virol 84:3935-48. 2010
  10. doi request reprint The Diaphanous-related Formin FHOD1 associates with ROCK1 and promotes Src-dependent plasma membrane blebbing
    Sebastian Hannemann
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Biol Chem 283:27891-903. 2008

Collaborators

Detail Information

Publications48

  1. ncbi request reprint Functional characterization of HIV-1 Nef mutants in the context of viral infection
    Oliver T Fackler
    Department of Virology, University of Heidelberg, INF 324, D 69120 Heidelberg, Germany
    Virology 351:322-39. 2006
    ..The results emphasize that multiple activities govern Nef's effects on HIV replication and argue against a role of virion incorporation for Nef's activity as pathogenicity factor...
  2. ncbi request reprint Modulation of the immunological synapse: a key to HIV-1 pathogenesis?
    Oliver T Fackler
    Oliver T Fackler is at the Department of Virology, University of Heidelberg, D 69120 Heidelberg, Germany
    Nat Rev Immunol 7:310-7. 2007
    ..We propose that modulating lymphocyte signalling, apoptosis and intracellular trafficking ensures efficient spread of the virus in the hostile environment of the immune system...
  3. ncbi request reprint Interactions of human retroviruses with the host cell cytoskeleton
    Oliver T Fackler
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Curr Opin Microbiol 9:409-15. 2006
    ..Exciting progress has been made in understanding how HIV and HTLV polarize actin and also control microtubule organization to spread from donor to target cells in close cell-contacts termed virological synapses...
  4. ncbi request reprint Modulation of specific surface receptors and activation sensitization in primary resting CD4+ T lymphocytes by the Nef protein of HIV-1
    Oliver T Keppler
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Leukoc Biol 79:616-27. 2006
    ..Our data support the involvement of modulation of a defined set of cell surface receptors and sensitization to activation rather than an autonomous activation function in the role of Nef in HIV-1 pathogenesis...
  5. pmc Expression of Nef downregulates CXCR4, the major coreceptor of human immunodeficiency virus, from the surfaces of target cells and thereby enhances resistance to superinfection
    Stephanie Venzke
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Virol 80:11141-52. 2006
    ..The downregulation of chemokine coreceptors is a conserved activity of Nef to modulate infected cells, an important functional consequence of which is an enhanced resistance to HIV superinfection...
  6. ncbi request reprint Specific and distinct determinants mediate membrane binding and lipid raft incorporation of HIV-1(SF2) Nef
    Simone I Giese
    Department of Virology, University of Heidelberg, 69120 Heidelberg, Germany
    Virology 355:175-91. 2006
    ....
  7. ncbi request reprint The HIV-1 pathogenicity factor Nef interferes with maturation of stimulatory T-lymphocyte contacts by modulation of N-Wasp activity
    Claudia Haller
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Biol Chem 281:19618-30. 2006
    ..Together, our results demonstrate that Nef alters both the amount and composition of signaling microclusters. We propose modulation of actin dynamics as an important mechanism for Nef-induced alterations of TCR signaling...
  8. pmc The Nef protein of human immunodeficiency virus is a broad-spectrum modulator of chemokine receptor cell surface levels that acts independently of classical motifs for receptor endocytosis and Galphai signaling
    Nico Michel
    Department of Virology, University of Heidelberg, D 69120 Heidelberg, Germany
    Mol Biol Cell 17:3578-90. 2006
    ..Nef uses a mechanism that is distinct from well-established pathways orchestrating CKR metabolism and offers an interesting tool to study the multifaceted biology of CKRs...
  9. pmc Lentiviral Nef proteins utilize PAK2-mediated deregulation of cofilin as a general strategy to interfere with actin remodeling
    Bettina Stolp
    Department of Infectious Diseases, Virology, University of Heidelberg, INF 324, 69120 Heidelberg, Germany
    J Virol 84:3935-48. 2010
    ....
  10. doi request reprint The Diaphanous-related Formin FHOD1 associates with ROCK1 and promotes Src-dependent plasma membrane blebbing
    Sebastian Hannemann
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Biol Chem 283:27891-903. 2008
    ..These results define a role for endogenous FHOD1 in SH4 domain-induced blebbing and suggest that its activity is regulated by ROCK1 in a Src-dependent manner...
  11. pmc Antagonism of CD317 restriction of human immunodeficiency virus type 1 (HIV-1) particle release and depletion of CD317 are separable activities of HIV-1 Vpu
    Christine Goffinet
    Department of Infectious Diseases, Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Virol 84:4089-94. 2010
    ..Thus, Vpu can efficiently antagonize virion tethering in the absence of CD317 degradation...
  12. ncbi request reprint The Nef protein of human immunodeficiency virus establishes superinfection immunity by a dual strategy to downregulate cell-surface CCR5 and CD4
    Nico Michel
    Department of Virology, University of Heidelberg, Germany
    Curr Biol 15:714-23. 2005
    ..This state of superinfection immunity counteracts potentially detrimental consequences for the infected cell and facilitates high-level replication and viral spread in the host...
  13. pmc Human immunodeficiency virus type 1 Nef activates p21-activated kinase via recruitment into lipid rafts
    Ellen Krautkrämer
    Abteilung Virologie, Universitatsklinikum Heidelberg, D 69120 Heidelberg, Germany
    J Virol 78:4085-97. 2004
    ..Together, these data suggest that Nef induces signal transduction via the recruitment of a signaling machinery including Pak into lipid rafts, thereby mimicking a physiological cellular mechanism to initiate the TCR cascade...
  14. pmc β-TrCP is dispensable for Vpu's ability to overcome the CD317/Tetherin-imposed restriction to HIV-1 release
    Hanna Mari Tervo
    Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany
    Retrovirology 8:9. 2011
    ..Recently, it has been suggested that β-TrCP is also critically involved in Vpu's ability to overcome the CD317-mediated virion release block...
  15. doi request reprint HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin
    Joëlle V Fritz
    Department of Infectious Diseases, Virology, University of Heidelberg, INF 324, 69120 Heidelberg, Germany
    Virology 424:33-44. 2012
    ..We conclude that (i) lipid raft incorporation is dispensable for Vpu-mediated CD317 antagonism and (ii) Vpu does not antagonize CD317 by extraction from lipid rafts...
  16. pmc Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo
    Stefanie Homann
    Department of Virology, University of Heidelberg, Germany
    Retrovirology 6:6. 2009
    ....
  17. pmc Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation
    Jana Stastna
    Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany
    Cell Res 22:728-45. 2012
    ..We propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility...
  18. doi request reprint Nef does not inhibit F-actin remodelling and HIV-1 cell-cell transmission at the T lymphocyte virological synapse
    Claudia Haller
    Department of Infectious Diseases, Virology, University Hospital Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Eur J Cell Biol 90:913-21. 2011
    ..Nef therefore selectively interferes with actin remodelling processes involved in antiviral host cell defense while actin driven processes that promote virus propagation remain unaltered...
  19. doi request reprint HIV-1 Nef interferes with host cell motility by deregulation of Cofilin
    Bettina Stolp
    Department of Virology, Im Neuenheimer Feld 324, University of Heidelberg, Heidelberg 69120, Germany
    Cell Host Microbe 6:174-86. 2009
    ..We conclude that HIV-1 Nef alters Pak2 function, which directly or indirectly inactivates cofilin, thereby restricting migration of infected T lymphocytes as part of a strategy to optimize immune evasion and HIV-1 replication...
  20. pmc HIV-1 Vpu blocks recycling and biosynthetic transport of the intrinsic immunity factor CD317/tetherin to overcome the virion release restriction
    Sarah Schmidt
    Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany
    MBio 2:e00036-11. 2011
    ..Treatment of cells with the malaria drug primaquine resulted in a CD317 trafficking defect that mirrored that induced by Vpu. Importantly, primaquine could functionally replace Vpu as a CD317 antagonist and rescue HIV-1 particle release...
  21. ncbi request reprint FHOD1 coordinates actin filament and microtubule alignment to mediate cell elongation
    Judith E Gasteier
    Abteilung Virologie, Universitatsklinikum Heidelberg, INF 324, D 69120 Heidelberg, Germany
    Exp Cell Res 306:192-202. 2005
    ..Thus, sustained cell elongation was a consequence of FHOD1-mediated actin-microtubule coordination. These results suggest filament coordination as a conserved function of mammalian DRFs...
  22. pmc Inhibition of T-cell receptor-induced actin remodeling and relocalization of Lck are evolutionarily conserved activities of lentiviral Nef proteins
    Jochen M Rudolph
    Department of Virology, University of Heidelberg, INF 324, 69120 Heidelberg, Germany
    J Virol 83:11528-39. 2009
    ..These results establish alterations of early TCR signaling events at the IS, including F-actin remodeling and relocalization of Lck, as evolutionary conserved activities of highly divergent lentiviral Nef proteins...
  23. pmc Human immunodeficiency virus type 1 Nef recruits the guanine exchange factor Vav1 via an unexpected interface into plasma membrane microdomains for association with p21-activated kinase 2 activity
    Susanne Rauch
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Virol 82:2918-29. 2008
    ..These results identify Vav1 as a relevant component of the Nef-PAK2 signalosome and provide a molecular basis for the role of F195 in formation of a catalytically active Nef-PAK2 complex...
  24. doi request reprint HIV-1 at the immunological and T-lymphocytic virological synapse
    Claudia Haller
    Department of Virology, University of Heidelberg, INF 324, D 69120 Heidelberg, Germany
    Biol Chem 389:1253-60. 2008
    ..The careful dissection of these opposing effects will contribute to our understanding of retroviral spread and cellular signal transduction machineries...
  25. doi request reprint SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells
    Hanna Mari Baldauf
    Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany
    Nat Med 18:1682-7. 2012
    ..Together, these findings may unveil new ways to interfere with the immune evasion and T cell immunopathology of pandemic HIV-1...
  26. pmc Involvement of clathrin-mediated endocytosis in human immunodeficiency virus type 1 entry
    Jessica Daecke
    Abteilung Virologie, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Virol 79:1581-94. 2005
    ..We conclude that dynamin-dependent, clathrin-mediated endocytosis can lead to productive entry of HIV in HeLa cells, suggesting this pathway as an alternative route of virus entry...
  27. doi request reprint HIV-1 antagonism of CD317 is species specific and involves Vpu-mediated proteasomal degradation of the restriction factor
    Christine Goffinet
    Department of Virology, University of Heidelberg, Heidelberg, Germany
    Cell Host Microbe 5:285-97. 2009
    ..Thus, HIV-1 Vpu suppresses the expression of the CD317 antiviral factor in human cells, and the species-specific resistance to this suppression may guide the development of small animal models of HIV infection...
  28. pmc Rodent cells support key functions of the human immunodeficiency virus type 1 pathogenicity factor Nef
    Oliver T Keppler
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Virol 79:1655-65. 2005
    ....
  29. pmc Human immunodeficiency virus type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains
    Britta Brugger
    Heidelberg University Biochemistry Center BZH, Heidelberg, Germany
    Retrovirology 4:70. 2007
    ..Since Nef modulates the protein composition and function of membrane microdomains we tested here if Nef also has the propensity to alter microdomain lipid composition...
  30. pmc Human endogenous retrovirus HERV-K(HML-2) encodes a stable signal peptide with biological properties distinct from Rec
    Alessia Ruggieri
    Department of Human Genetics, Medical Faculty, University of Saarland, Homburg, Germany
    Retrovirology 6:17. 2009
    ..The human endogenous retrovirus HERV-K(HML-2) family is associated with testicular germ cell tumors (GCT). Various HML-2 proviruses encode viral proteins such as Env and Rec...
  31. pmc Restrictions to HIV-1 replication in resting CD4+ T lymphocytes
    Xiaoyu Pan
    Department of Infectious Diseases, Virology, University Hospital Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Cell Res 23:876-85. 2013
    ..This review summarizes our current molecular and pathophysiological understanding of the multi-faceted interactions of HIV-1 with resting CD4(+) T lymphocytes. ..
  32. pmc Cell motility through plasma membrane blebbing
    Oliver T Fackler
    Department of Virology, University of Heidelberg, 69120 Heidelberg, Germany
    J Cell Biol 181:879-84. 2008
    ..The importance of blebbing as a functional protrusion is underscored by the existence of multiple molecular mechanisms that govern actin-mediated bleb retraction...
  33. ncbi request reprint Live and let die: Nef functions beyond HIV replication
    Oliver T Fackler
    Department of Virology, University of Heidelberg, D 69120 Heidelberg, Germany
    Immunity 16:493-7. 2002
    ..By targeting the T cell receptor, Nef may not only prime viral replication but, more importantly, ensure viral survival through distinct mechanisms of immune evasion and antiapoptosis...
  34. pmc HIV-1 Nef: a multifaceted modulator of T cell receptor signaling
    Libin Abraham
    Department of Infectious Diseases, Virology, University Hospital Heidelberg, INF 324, Heidelberg, 69120, Germany
    Cell Commun Signal 10:39. 2012
    ..We also discuss the implications of these alterations in the context of HIV-1 infection and in light of current concepts of TCR signal transduction...
  35. ncbi request reprint Activation of the Rac-binding partner FHOD1 induces actin stress fibers via a ROCK-dependent mechanism
    Judith E Gasteier
    Institute for Hygiene, Department of Virology, University of Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 278:38902-12. 2003
    ..These results indicate that FHOD1 acts as an effector of Rac in actin rearrangements and transcriptional regulation and may provide a link for the Rac-dependent activation of the Rho cascade...
  36. pmc Positive feedback between Dia1, LARG, and RhoA regulates cell morphology and invasion
    Thomas M Kitzing
    Institute of Pharmacology, University of Heidelberg, 69120 Heidelberg, Germany
    Genes Dev 21:1478-83. 2007
    ..Our results reveal that Dia1 is necessary for LPA-stimulated Rho/ROCK signaling and bleb-associated cancer cell invasion. Thus, Dia1-dependent RhoA activation constitutes a positive feedback mechanism to modulate cell behavior...
  37. ncbi request reprint Novel (n)PKC kinases phosphorylate Nef for increased HIV transcription, replication and perinuclear targeting
    Dietlinde Wolf
    University of Miami, Miller School of Medicine, Department of Microbiology and Immunology, BCRI 739, Miami, FL 33136, USA
    Virology 370:45-54. 2008
    ..We conclude that the activation of nPKCs by Nef is required to increase viral replication/infectivity and direct the subcellular localization of Nef...
  38. pmc Nef binds p6* in GagPol during replication of human immunodeficiency virus type 1
    Luciana J Costa
    Department of Medicine, University of California San Francisco, UCSF Mt Zion Cancer Center, 2340 Sutter Street, San Francisco, CA 94115, USA
    J Virol 78:5311-23. 2004
    ..Our data demonstrate that the retention of Nef in the intermediate compartment interferes with viral replication and suggest a new role for Nef in the production of HIV-1...
  39. pmc Construction and characterization of a fluorescently labeled infectious human immunodeficiency virus type 1 derivative
    Barbara Muller
    Abteilung Virologie, Universitätklinikum Heidelberg, Germany
    J Virol 78:10803-13. 2004
    ..The fluorescent virus derivative is a useful tool for investigating the interaction of HIV with live cells...
  40. ncbi request reprint Replication of M-tropic HIV-1 in activated human intestinal lamina propria lymphocytes is the main reason for increased virus load in the intestinal mucosa
    Sheriff Aziz
    Internal Medicine II, University Saarland, Homburg Saar, Germany
    J Acquir Immune Defic Syndr 38:23-30. 2005
    ..Thus, the elevated activation state of target cells in the intestine and not the expression of the homing marker CD103 is directly linked to massive HIV production...
  41. ncbi request reprint Construction and characterization of an HIV-1 group O infectious molecular clone and analysis of vpr- and nef-negative derivatives
    Denis M Tebit
    Abteilung Virologie, Universitatsklinikum Heidelberg, D 69ab0, Germany
    Virology 326:329-39. 2004
    ..5. Virus obtained from pCMO2.5 replicated with similar kinetics as the parental O-type isolate in both PBMC and MDM, making this proviral clone a valuable tool for further studies on functional characteristics of HIV-1 group O viruses...
  42. ncbi request reprint The Pro78 residue regulates the capacity of the human immunodeficiency virus type 1 Nef protein to inhibit recycling of major histocompatibility complex class I molecules in an SH3-independent manner
    Nicoletta Casartelli
    Division of Immunology and Infectious Disease, Children s Hospital Bambino Gesu, Piazza S Onofrio 4, 00165 Rome, Italy
    J Gen Virol 87:2291-6. 2006
    ..In addition, it is demonstrated that P72 and P75, and thus the SH3-binding capacity, are fully dispensable for Nef activity on MHC-I...
  43. ncbi request reprint SH4-domain-induced plasma membrane dynamization promotes bleb-associated cell motility
    Stella Tournaviti
    Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    J Cell Sci 120:3820-9. 2007
    ....
  44. pmc HIV-1 Nef employs two distinct mechanisms to modulate Lck subcellular localization and TCR induced actin remodeling
    Claudia Haller
    Abteilung Virologie, Universitatsklinikum Heidelberg, Heidelberg, Germany
    PLoS ONE 2:e1212. 2007
    ..Thus, Nef affects IS function via multiple independent mechanisms to optimize virus replication in the infected host...
  45. ncbi request reprint CTL-mediated selective pressure influences dynamic evolution and pathogenic functions of HIV-1 Nef
    Takamasa Ueno
    Division of Viral Immunology, Center for AIDS Research, Kumamoto University, Kumamoto, Japan
    J Immunol 180:1107-16. 2008
    ..These results highlight CTL immunosurveillance as important modulators of Nef's biological activity in the infected host...
  46. ncbi request reprint Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery
    Matthias Geyer
    Department of Medicine, University of California, San Francisco, California 94143 0703, USA
    J Biol Chem 277:28521-9. 2002
    ..Finally, blocking the expression of V1H decreased the enhancement of virion infectivity by Nef. Thus, V1H can function as an adaptor for interactions between Nef and AP-2...
  47. ncbi request reprint Biochemical characterization of the diaphanous autoregulatory interaction in the formin homology protein FHOD1
    André Schönichen
    Max Planck Institute for Molecular Physiology, Department of Physical Biochemistry, D 44227 Dortmund, Germany
    J Biol Chem 281:5084-93. 2006
    ....
  48. pmc Subunit H of the V-ATPase involved in endocytosis shows homology to beta-adaptins
    Matthias Geyer
    Howard Hughes Medical Institute, Department of Medicine, University of California at San Francisco, California 94143 0703, USA
    Mol Biol Cell 13:2045-56. 2002
    ..Based on recent structural analysis, our results suggest that the di-leucine-binding domain consists of a HEAT or ARM repeat protein fold...